Pengjie Li, Peng Chen, Fukang Qi, Jinyun Shi, Wenjie Zhu, Jiashuo Li, Peng Zhang, Han Xie, Lina Li, Mengcheng Lei, Xueqing Ren, Wenhui Wang, Liang Zhang, Xufu Xiang, Yiwei Zhang, Zhaolong Gao, Xiaojun Feng, Wei Du, Xin Liu, Limin Xia, Bi-Feng Liu, Yiwei Li
Phase separation inside mammalian cells regulates the formation of the biomolecular condensates that are related to gene expression, signalling, development and disease. However, a large population of endogenous condensates and their candidate phase-separating proteins have yet to be discovered in a quantitative and high-throughput manner. Here we demonstrate that endogenously expressed biomolecular condensates can be identified across a cell's proteome by sorting proteins across varying oligomeric states. We employ volumetric compression to modulate the concentrations of intracellular proteins and the degree of crowdedness, which are physical regulators of cellular biomolecular condensates...
March 18, 2024: Nature Chemistry