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American Journal of Translational Research

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https://www.readbyqxmd.com/read/27904713/farnesoid-x-receptor-ligand-cdca-suppresses-human-prostate-cancer-cells-growth-by-inhibiting-lipid-metabolism-via-targeting-sterol-response-element-binding-protein-1
#1
Nian Liu, Jun Zhao, Jinguo Wang, Haolin Teng, Yaowen Fu, Hang Yuan
AIM: A wealth of studies have demonstrated that abnormal cellular lipid metabolism plays an important role in prostate cancer (PCa) development. Therefore, manipulating lipid metabolism is a potential PCa therapy strategy. In this study, our goal is to investigate the role of farnesoid X receptor (FXR) in regulating the proliferation and lipid metabolism of human PCa cells following its ligand chenodexycholic acid (CDCA) treatment. METHODS: Oil Red O was used to stain lipid contents in PCa cells, and siRNA knockdown was performed to deplete FXR expression...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904712/hyperbaric-oxygen-protects-mandibular-condylar-chondrocytes-from-interleukin-1%C3%AE-induced-apoptosis-via-the-pi3k-akt-signaling-pathway
#2
Hang Chen, Gaoyi Wu, Qi Sun, Yabing Dong, Huaqiang Zhao
Objectives: Mandibular condylar chondrocyte apoptosis is mainly responsible for the development and progression of temporomandibular joint osteoarthritis (TMJ-OA). Interleukin-1β (IL-1β) generally serves an agent that induces chondrocyte apoptosis. Hyperbaric oxygen (HBO) treatment increases proteoglycan synthesis in vivo. We explore the protective effect of HBO on IL-1β-induced mandibular condylar chondrocyte apoptosis in rats and the potential molecular mechanisms. Methods: Chondrocytes were isolated from the TMJ of 3-4-week old Sprague-Dawley rats...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904711/naringin-promotes-osteogenic-differentiation-of-bone-marrow-stromal-cells-by-up-regulating-foxc2-expression-via-the-ihh-signaling-pathway
#3
Fei-Xiang Lin, Shi-Xin Du, De-Zhong Liu, Qin-Xiao Hu, Guo-Yong Yu, Chu-Cheng Wu, Gui-Zhou Zheng, Da Xie, Xue-Dong Li, Bo Chang
Naringin is an active compound extracted from Rhizoma Drynariae, and studies have revealed that naringin can promote proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs). In this study, we explored whether naringin could promote osteogenic differentiation of BMSCs by upregulating Foxc2 expression via the Indian hedgehog (IHH) signaling pathway. BMSCs were cultured in basal medium, basal medium with naringin, osteogenic induction medium, osteogenic induction medium with naringin and osteogenic induction medium with naringin in the presence of the IHH inhibitor cyclopamine (CPE)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904710/the-comt-rs165599-gene-polymorphism-contributes-to-chemotherapy-induced-cognitive-impairment-in-breast-cancer-patients
#4
Huaidong Cheng, Wen Li, Chen Gan, Bo Zhang, Qianqian Jia, Kai Wang
The present study aimed to investigate the effect of genetic polymorphisms of catechol-O-methyl transferase (COMT), apolipoprotein E (APOE), and brain derived neurotrophic factor (BDNF) on the modulation of the chemotherapy-induced cognitive impairment (CICI) in breast cancer patients. Eighty triple negative breast cancer (TNBC) and 165 non-triple negative breast cancer (NTNBC) patients were selected, and subjected to a number of neuropsychological tests, including memory questionnaires, before and after chemotherapy...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904709/braf-and-epithelial-mesenchymal-transition-in-papillary-thyroid-carcinoma-challenging-the-roles-of-snail-and-e-cadherin
#5
Brendon Mitchell, Dominick Leone, Shi Yang, Ashraf Khan, Meera Mahalingam, Jagdish Dhingra
OBJECTIVE: In papillary thyroid carcinoma (PTC), while the role of BRAF is well established, the contribution of BRAF to epithelial-mesenchymal transition is not. STUDY DESIGN/SETTING: To elucidate the relationship between BRAF, surrogates of epithelial-mesenchymal transition (Snail, E-cadherin) and established histopathologic prognosticators in papillary thyroid carcinoma. SUBJECTS/METHODS: In this IRB approved cross-sectional study, 50 cases of archived annotated PTC samples were retrieved and immunohistochemically stained for Snail and E-cadherin protein...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904708/overexpression-of-carboxypeptidase-a4-cpa4-is-associated-with-poor-prognosis-in-patients-with-gastric-cancer
#6
Lichao Sun, Chunguang Guo, Hebao Yuan, Joseph Burnett, Jian Pan, Zhihua Yang, Yuliang Ran, Ila Myers, Duxin Sun
CarboxypeptidaseA4 (CPA4) is a zinc-containing exopeptidases, and its aberrant expression has been implicated in cancer development and progression. However, few studies have investigated the association between CPA4 over-expression and clinical significance in gastric cancer (GC). In this study, we employed immunohistochemistry to evaluate the expression of CPA4 in gastric cancer tissues, and found that elevated CPA4 expression was detected in 64% (n=100) of primary GCs, but was weak or no staining in the normal mucosa...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904707/prognostic-significance-of-%C3%AE-2-adrenergic-receptor-expression-in-non-small-cell-lung-cancer
#7
Tomohiro Yazawa, Kyoichi Kaira, Kimihiro Shimizu, Akira Shimizu, Keita Mori, Toshiteru Nagashima, Yoichi Ohtaki, Tetsunari Oyama, Akira Mogi, Hiroyuki Kuwano
BACKGROUND: The β2-Adrenergic receptor (β2-AR) is associated with tumor growth and progression. However, the clinical significance of β2-AR expression in patients with non-small cell lung cancer (NSCLC) remains unclear. METHODS: Three hundred twenty-eight patients with surgically resected NSCLC were retrospectively investigated. Tumor sections were stained by immunohistochemistry for assessing β2-AR and Ki-67 expression and microvessel density (MVD), which was using CD34 levels...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904706/the-central-role-of-hotair-in-the-malignancy-of-cd44-human-hypopharyngeal-carcinoma-cells
#8
Hongxia Cheng, Xinhua Cui, Ying Guo, Lintao Gu, Yaning Wang, Qirong Wang, Hui Liang
Human hypopharyngeal carcinoma is one of the most common malignant tumors. CD44 could serve as a molecular marker to screen for cancer stem cells (CSCs) in hypopharyngeal cancer. The aim of this study was to identify the role of HOX transcript antisense RNA (HOTAIR) on cell proliferation and invasion in CD44+ FADU cells (human hypopharyngeal carcinoma cells). We also explored the underlying mechanism contributing to HOTAIR's observed effects. CD44+ FADU cells were sorted and purified by flow cytometry and infected with lentivirus stably expressing HOTAIR shRNA...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904705/paclitaxel-suppresses-collagen-induced-arthritis-a-reevaluation
#9
Yi Zhao, Zhi-Fang Chang, Ru Li, Zhan-Guo Li, Xiao-Xia Li, Lin Li
OBJECTIVE: To reevaluate the suppressive effect of paclitaxel (PTX) liposome on collagen-induced arthritis (CIA) in rats and explore its mechanisms. METHODS: Female Lewis rats were immunized with bovine type II collagen (CII) to induce arthritis. The rats with CIA were randomly divided into three groups: 5% GS control group, 2.5 mg/kg PTX treatment group and 1 mg/kg methotrexate (MTX) positive control group. The drugs were administered by intraperitoneal injection on the second day after arthritis onset...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904704/lncrna-sox2ot-overexpression-serves-as-a-poor-prognostic-biomarker-in-gastric-cancer
#10
Yuanyuan Zhang, Rui Yang, Jianchun Lian, Haiyan Xu
Sox2 overlapping transcript (Sox2ot) serves as an unfavorable prognostic factor in lung cancer and hepatocellular carcinoma, but the clinical significance and biological function of Sox2ot in gastric cancer is still unknown. The objective of our research is to explore the significance of Sox2ot in gastric cancer. Quantitative real-time polymerase chain reaction was performed to determine Sox2ot in gastric cancer tissues and cell lines. Furthermore, the Sox2ot status in one hundred and thirty two gastric cancer samples was detected to analyzed correlations between Sox2ot and clinicopathological features...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904703/lncrna-pvt1-overexpression-is-a-poor-prognostic-biomarker-and-regulates-migration-and-invasion-in-small-cell-lung-cancer
#11
Chengsuo Huang, Shuguang Liu, Huijun Wang, Zicheng Zhang, Qing Yang, Fang Gao
PVT1 has been suggested as playing important roles in diverse biological processes including tumorigenesis. However, the clinical significance and biological function of PVT1 in small cell lung cancer (SCLC) is still unclear. The purpose of this study is to identify the role of PVT1 in SCLC. The expression of PVT1 was examined in SCLC tissues and cell lines through real-time PCR. Meanwhile, the relationship of PVT1 expression levels with clinical characteristics of 120 SCLC patients was analyzed. Univariate and multivariate analyses were performed to determine the association between PVT1 expression and prognosis of SCLC patient...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904702/ethanol-extracts-from-portulaca-oleracea-l-attenuated-ischemia-reperfusion-induced-rat-neural-injury-through-inhibition-of-hmgb1-induced-inflammation
#12
Chenggang Zheng, Chen Liu, Wanyin Wang, Gusheng Tang, Liwei Dong, Juan Zhou, Zhengrong Zhong
It is well demonstrated that the high mobility group box 1 (HMGB1) mediated inflammation has been implicated as one of the important causes for brain damage induced by cerebral ischemia/reperfusion (I/R). In the present study, we assessed the neuro-protective and anti-inflammation effects of the ethanol extracts from Portulaca oleracea L. (EEPO) against cerebral I/R injury in the rat transient middle cerebral artery occlusion (tMCAO) model. Rats were administrated with their respective treatment for 7 days before the MCA occlusion...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904701/sirt6-suppresses-mitochondrial-defects-and-cell-death-via-the-nf-%C3%AE%C2%BAb-pathway-in-myocardial-hypoxia-reoxygenation-induced-injury
#13
Ming-Yue Cheng, You-Wei Cheng, Jun Yan, Xiao-Qin Hu, Hui Zhang, Zhi-Rong Wang, Qin Yin, Wei Cheng
The present study explored changes of the SIRT6/NF-κB pathway in myocardial hypoxia/reoxygenation induced injury and the effects on mitochondrial damage and myocardial damage by regulating SIRT6. SIRT6 expression decreased and NF-κB expression increased in H9c2 cells during hypoxic injury. Cell death and mitochondrial defects paralleled mPTP opening, and a decrease in ΔΨm occurred in hypoxic myocytes compared with normoxic control cells in annexin V and propidium iodide staining and TUNEL results. These effects were suppressed in cells overexpressing SIRT6, but reemerged in cells expressing the SIRT6 mutant...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904700/detection-of-gene-copy-number-alterations-in-dcis-and-invasive-breast-cancer-by-qm-fish
#14
Aifeng Pan, Yawei Zhou, Kun Mu, Yansong Liu, Feifei Sun, Peng Li, Li Li
The exact roles of copy number alteration (CNA) in initiation, progression and immunotherapy of breast cancer and the genomic alterations behind progression from ductal carcinoma in situ (DCIS) to invasive carcinoma remain unknown. Quantitative multi-gene fluorescence in situ hybridization (QM-FISH) opens a possibility of large scale genomic analysis of specific deletions and amplifications with high-resolution at one cell level. We detected CNAs of 30 genes using QM-FISH and analyzed their association with clinicopathological parameters and patients' outcomes in 66 breast cancers with synchronous invasive carcinoma and DCIS...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904699/generation-of-induced-pluripotent-stem-cells-with-high-efficiency-from-human-embryonic-renal-cortical-cells
#15
Ling Yao, Ruifang Chen, Pu Wang, Qi Zhang, Hailiang Tang, Huaping Sun
Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) emerges as a prospective therapeutic angle in regenerative medicine and a tool for drug screening. Although increasing numbers of iPSCs from different sources have been generated, there has been limited progress in yield of iPSC. Here, we show that four Yamanaka factors Oct4, Sox2, Klf4 and c-Myc can convert human embryonic renal cortical cells (hERCCs) to pluripotent stem cells with a roughly 40-fold higher reprogramming efficiency compared with that of adult human dermal fibroblasts...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904698/microrna-665-suppressed-the-invasion-and-metastasis-of-osteosarcoma-by-directly-inhibiting-rab23
#16
Chenhui Dong, Quanyin Du, Zimin Wang, Yu Wang, Siyu Wu, Aimin Wang
MicroRNAs (miRNAs) are small, short and noncoding RNAs that regulate gene expression posttranscriptionally. Increasing evidences have demonstrated that deregulated expression of miRNAs is found in osteosarcoma. In this study, we demonstrated that miR-665 was downregulated in osteosarcoma tissues compared to non-tumorous tissues. The overall survival (OS) of osteosarcoma patients with low miR-665 expression was lower than that of these patients with high miR-665 expression. Ectopic expression of miR-665 suppressed the osteosarcoma cell proliferation, EMT and invasion...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904697/inhibitory-effect-of-bufalin-on-retinoblastoma-cells-hxo-rb44-via-the-independent-mitochondrial-and-death-receptor-pathway
#17
Qingfeng Meng, Yan Zhao, LiXin An, Xia Li, Ping Liu
Cinobufacini (Huachansu) is a Chinese medicine prepared from the skin of Bufo bufo gargarizans Cantor (Bufonidae), and has long been used in traditional Chinese medicine. In the present study, the anti-retinoblastoma constituent bufalin obtained from Cinobufacini was investigated. Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904696/a-study-on-the-correlation-between-mthfr-promoter-methylation-and-diabetic-nephropathy
#18
Xiao-Hui Yang, Ren-Fang Cao, Yang Yu, Miao Sui, Tao Zhang, Jing-Yi Xu, Xiao-Mei Wang
OBJECTIVE: In order to observe the relationship between MTHFR promoter and DN, the determinations on MTHFR promoter methylation level and expression of HCY from DN patients have been carried out. METHODS: According to the Diabetes diagnosis and classification standard from WHO in 1999, 85 patients with DM diagnosed by Endocrinology and 30 healthy participants from our medical examination center were chosen as control specimen to study in this paper. All this specimen were divided into A, B, C and D four groups , which are corresponding simple diabetes mellitus group (SDM), early diabetic nephropathy group (EDN), clinical diabetic nephropathy group (CDN) and normal control group...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904695/overexpression-of-akip1-predicts-poor-prognosis-of-patients-with-breast-carcinoma-and-promotes-cancer-metastasis-through-akt-gsk-3%C3%AE-snail-pathway
#19
Dan Mo, Xinning Li, Chunhong Li, Junrong Liang, Tian Zeng, Naiwei Su, Qipei Jiang, Jingjing Huang
Recent evidence has demonstrated that A kinase interacting protein 1 (AKIP1), a molecular regulator of protein kinase A, was overexpressed in breast cancer. However, the prognostic and biological role of AKIP1 in breast cancer is still elusive. The purpose of our study was to elucidate the role and molecular mechanism of AKIP1 in breast cancer development. The mRNA levels of AKIP1 in breast cancer and paired normal breast tissues were examined by quantitative real-time PCR. The relationship of AKIP1 expression with clinicopathological characteristics and clinical prognosis of breast cancer patients was investigated...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904694/soluble-flt-1-improves-the-repair-of-ankle-joint-injury-in-rats
#20
Jing Tian, Bing Xie, Liangbi Xiang, Yong Zhao, Dapeng Zhou
The ankle injuries create great pain to a great number of patients worldwide. Past studies have focused on the development of practical treatments to relieve pain and improve recovery, but the molecular mechanisms underlying the ankle injuries, especially the local inflammation in the damaged ankle joint, have been rarely studied. Moreover, although reduction of production and secretion of pro-inflammatory cytokines may reduce the pain and promote the recovery, a practical approach is currently lacking. Here, we detected significantly higher levels of placental growth factor (PLGF) and pro-inflammatory cytokines in the joint fluid from the patients of acute ankle joint injury (AAJI)...
2016: American Journal of Translational Research
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