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American Journal of Translational Research

Wenqin Xiao, Weiliang Jiang, Kai Li, Yangyang Hu, Sisi Li, Li Zhou, Rong Wan
[This corrects the article on p. 3842 in vol. 9, PMID: 28861174.].
2018: American Journal of Translational Research
Charlotte von Horn, Thomas Minor
Normothermic machine perfusion can decrease reperfusion injury in renal transplantation. Clinical procurement logistics include retrieval and initial transport of the graft using static cold storage. Therefore, use and benefits of brief normothermic reconditioning by machine perfusion should be investigated in the initially cold preserved graft. Porcine kidneys (6 per group) were retrieved 20 min after cardiac standstill. After 20 h of static cold preservation some grafts were put on a machine perfusion circuit and normothermically perfused for 2 h at 35°C (NMP)...
2018: American Journal of Translational Research
Xiongjie Zhu, Jiale Wang, Lihua Li, Lian Deng, Jinting Wang, Lu Liu, Rong Zeng, Qien Wang, Yanfang Zheng
Although an increasing number of findings have proven that glutathione peroxidase 3 (GPX3) is methylated and down-regulated in various cancers, the underlying mechanism of its occurrence in esophageal squamous cell carcinoma (ESCC) remains unknown. In the present study, we found that the methylation rate in advanced cancers was significantly higher than that in early stage cancers by a methylation-specific polymerase chain reaction. Furthermore, the proliferation and migration capacities of KYSE-510 cells were inhibited after up-regulating GPX3 expression by GPX3 lentivirus transfection...
2018: American Journal of Translational Research
Fangfang Xu, Yong Yang, Tao Yang, Taiqiang Dai, Xiaoxi Shao, Haiyan Xu, Ran An, Yanpu Liu, Bin Liu
The current study aims to assess the efficacy of allogenic adipose-derived stem cells (ADSCs) together with platelet-rich fibrin (PRF) for the treatment of rabbit ear cartilage defects. For this study, 12 New Zealand white rabbits were randomly allocated into 4 groups. Two full-thickness cartilage defects were created in the rabbit ears. Group 1 was left untreated; Group 2 was treated with allogenic ADSCs, Group 3 was treated with PRF; Group 4 was treated with allogenic ADSCs and PRF. Macroscopic observation, hematoxylin and eosin staining, and Alcian blue staining after 3 months suggested that the allogenic ADSCs/PRF significantly accelerated cartilage regeneration compared to other groups and this was associated with increased expression of collagen II relative to the other groups...
2018: American Journal of Translational Research
Jian Zhou, Handong Wang, Ruiming Shen, Jiang Fang, Youqin Yang, Wei Dai, Yihao Zhu, Mengliang Zhou
Mitoquinone (MitoQ) is a powerful mitochondrial-targeted antioxidant whose neuroprotective effects have been shown in a variety of animal models of neurological diseases. However, its roles in traumatic brain injury (TBI) remain unexplored. The primary objective of this study was to investigate the neuroprotection afforded by MitoQ in a mouse model of TBI, and the involvement of the Nrf2-ARE signaling pathway in the putative neuroprotective mechanism. Mice were randomly divided into four groups: sham group, TBI group, TBI + vehicle group, and TBI + MitoQ group...
2018: American Journal of Translational Research
Liang Zhou, Zuwei Li, Xiang Pan, Yulin Lai, Jing Quan, Liwen Zhao, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Shangqi Yang, Yaoting Gui, Yongqing Lai
BACKGROUND: RCC is a malignant tumor that originates from renal tubular epithelial cells, accounting for nearly 90% of renal malignancies and 3% of adult malignancies. It was reported that more than 30-40% of patients with early localized RCC still have recurrence and metastasis after receiving radical surgery. miRNAs are an endogenous non-coding small RNAs that play an important role in the regulation of tumor cell proliferation, differentiation and apoptosis. METHODS: In our study, RT-qPCR, CCK-8 assay, wound scratch assay, transwell assay and flow cytometry assay were designed to identify the expression and functions of miR-18a-5p in RCC...
2018: American Journal of Translational Research
Adheesh Bhandari, Erjie Xia, Yinghao Wang, Namita Sindan, Ranjan Kc, Yaoyao Guan, Yueh-Lung Lin, Xiaoshang Wang, Xiaohua Zhang, Ouchen Wang
Comparing diagnostic accuracy study between ultrasonography (US) guided fine-needle aspiration biopsy (FNAB) and core-needle biopsy (CNB) of the Sentinel lymph nodes (SLNs) in newly diagnosed invasive breast cancer patients. We selected 289 newly diagnosed invasive breast cancer patients from June 2015 to July 2017. Ultrasound (US) guided fine-needle aspiration cytology (FNA) and core-needle biopsy (CNB) was performed to identify patients with suspicious sentinel lymph node (SLN). Patients with a cortical thickness > 2 mm or atypical morphological characteristics were recommended FNA and CNB...
2018: American Journal of Translational Research
Chong Sun, Bin Wang, Jianmin Li, Junjie Shangguan, Matteo Figini, Kang Zhou, Liang Pan, Quanhong Ma, Zhuoli Zhang
BACKGROUND: Previous studies have shown the poor prognosis of metastatic breast cancer including bone metastasis. The early prediction and intervention of invasive breast carcinoma with bone metastasis are crucial to the outcomes of patients. The purpose of our study is to test the hypothesis that the collagen deposition of primary breast cancer can be used as a quantitative biomarker for the early prediction of bone metastasis. METHODS: A total of sixty breast cancer patients were included in our study, and the surgical specimens of these patients were divided into three groups: patients with no metastasis (group 1), lymph node metastasis (group 2), and bone metastasis (group 3)...
2018: American Journal of Translational Research
Aryadi Arsyad, Elke Sokoya, Geoffrey P Dobson
New pharmacotherapies are required to improve vessel graft protection and prevent vasoconstriction and spasm in CABG surgery. Previously we have studied adenosine (A) and lidocaine (L) relaxation in rat aortic rings, and reported a possible crosstalk between L relaxation and adenosine A2a receptor inhibition. The aim of the present study was to examine the effect of AL combination compared to A and L alone on relaxation in intact and denuded rat aortic rings and in guinea-pig pressurized mesenteric arterial segments...
2018: American Journal of Translational Research
Li Deng, Weixin Meng, Dandan Li, Dongyun Qiu, Siqing Wang, Hongyu Liu
This study aimed to investigate the effect of ozone on hypoxia, hemolysis and morphological change of blood from aortic dissection (AD) patients for providing preliminary evidence of application of ozonated autohemotherapy (Ozone-AHT) in AD patients. 20 AD patients and 20 healthy volunteers were consecutively included, and blood samples were collected from all participants and ozonized in vitro . PO2 , SO2 , malondialdehyde (MDA), superoxide dismutase (SOD), malformation percentage, morphology change and spatial distribution of filamentous actin (F-actin) in erythrocytes at different ozone concentrations were evaluated...
2018: American Journal of Translational Research
Zhuanglin Zeng, Bingxin Cao, Xiaopeng Guo, Weijuan Li, Songhai Li, Juan Chen, Wenping Zhou, Chuansheng Zheng, Yumiao Wei
Immunization with peptides derived from apolipoprotein B-100 (ApoB-100) has been shown to ameliorate atherosclerosis in apolipoprotein E knockout (ApoE-/- ) mice. However, the exact mechanism underlying the therapeutic effects remains elusive. To shed light on this mechanism, we immunized ApoE-/- mice that were fed a Western diet with either malondialdehyde-modified ApoB-100 peptide 210 (P210) emulsified in Freund's adjuvant or anti-malondialdehyde-modified P210 antibody (P210-Ab). Mice immunized with Freund's adjuvant or bovine serum albumin served as controls...
2018: American Journal of Translational Research
Kun Ling Ma, Yu Wu, Yang Zhang, Gui Hua Wang, Ze Bo Hu, Xiong Zhong Ruan
Non-alcoholic fatty liver disease (NAFLD), characterised by early lipid accumulation and subsequent inflammation in the liver, is becoming a worldwide challenge due to its increasing prevalence in developing and developed countries. This study aimed to investigate the role of CXC chemokine ligand 16 (CXCL16) and its receptor CXC chemokine receptor 6 (CXCR6) in NAFLD under inflammation. We used IL-1β stimulation in human hepatoblastoma cell line (HepG2) for in vitro studies and casein injection in apolipoprotein E knockout mice in vivo to induce inflammatory stress...
2018: American Journal of Translational Research
Gang Yu, Weili Sun, Yong Shen, Yongquan Hu, Henchao Liu, Weipeng Li, Yong Wang
In this study, we investigated the biological function of pyruvate kinase M2 (PKM2) and its regulation by deregulated microRNAs (miRNAs) in thyroid cancer (TC). The mRNA and protein expression of PKM2 was examined by quantitative reverse transcription PCR and western blot. The biological role of PKM2 was demonstrated through small interfering RNA-mediated knockdown experiments. The regulation of PKM2 by miR-148a and miR-326 was confirmed by western blot, dual luciferase activity assays, and rescue experiments...
2018: American Journal of Translational Research
Zhen-Gang Ji, Hai-Tao Jiang, Pei-Song Zhang
Glioma is the most common primary malignant brain tumor in adults. Forkhead box k1 (FOXK1) was reported to be dysregulated and play important roles in multiple human cancers. However, the expression pattern and roles of FOXK1 in glioma has never been investigated. In this study, we firstly observed that the expression of FOXK1 was significantly increased in glioma tissue samples and cell lines. Functional assays demonstrated that overexpression of FOXK1 promoted proliferation, cell cycle transition and inhibited apoptosis in glioma cell lines...
2018: American Journal of Translational Research
Xi Chen, Lingbing Zeng
Although the standard ginkgo biloba extract EGb 761 exhibits antioxidative, anti-apoptotic, and anticancer properties, there is no research focusing on the chemopreventive effects of EGb 761 in colorectal cancer (CRC). The present study investigated whether EGb 761 could increase 5-fluorouracil (5FU) sensitivity in CRC and its potential mechanism. We found that combined EGb 761 and 5FU treatment significantly elevated the chemosensitivity of CRC cells to 5FU in 5FU-resistant (5FUR) CRC cells, whereas no obvious cytotoxicity of EGb 761 was observed in parental cells...
2018: American Journal of Translational Research
Han Zhu, Meng Wang, Chengjun Zhao, Ruosong Li, Juan Yang, Guangchang Pei, Ting Ye, Xuezhi Zuo, Liu Liu, Octavia Ls Chong Lee Shin, Fengming Zhu, Jie Sun, Huzi Xu, Zhi Zhao, Chujin Cao, Yuxi Wang, Qian Yang, Gang Xu, Rui Zeng, Ying Yao
As a component of collagen II, glycosaminoglycan (GAG) has a relatively close relationship with bone metabolism. GAG and collagen II have been proven to promote connection of the bone trabecular structure. However, the exact mechanism remains unknown. In this study, we aimed to determine the concrete effect and the mechanism of GAG and collagen II on glucocorticoid-induced osteoporosis. We implanted prednisolone pellets subcutaneously in mice to mimic glucocorticoid-induced osteoporosis. GAG was administered intragastrically every day for 60 days...
2018: American Journal of Translational Research
Pingping Jiang, Lei Xiang, Zewei Chen, Hanqi Lu, Lin Zhou, Lebin Yang, Yanzhao Ji, Yanyan Liu, Xiaomin Sun, Yingfeng Deng, Xiaoli Nie, Ren Luo, Xiaoshan Zhao
OBJECTIVE: To evaluate the protective effect of catalpol against diabetic nephropathy in db/db mouse. METHODS: 8 week old C57BLKS/J db/db mice (type 2 diabetic mouse model) were divided into three groups to feed for 16 weeks on chow diet with or without catalpol supplementation. Their food intake, water consumption, body weight, and fasting glucose levels were recorded every 4 weeks. At the end of study, urine and blood samples were examined for several metabolic variables, and kidneys were harvested for structural characterization and microarray analysis...
2018: American Journal of Translational Research
Kun Chen, Zhiyuan Wu, Mengya Zang, Ce Wang, Yanmei Wang, Dongmei Wang, Yifan Ma, Chunfeng Qu
BACKGROUND: Glypican-3 (GPC3) is one of the key tissue markers that could discriminate malignant precancerous lesions from benign hepatic lesions in cirrhotic patients. We aimed to develop a GPC3 cancer vaccine to induce specific T cells to intervene in hepatocellular carcinoma (HCC) development. METHODS: Synthesizing mannosylated liposomes (LPMan) as vaccine delivery system, incorporating one Toll-like receptor (TLR)-7/8 agonist CL097 as adjuvant, we prepared a GPC3 nanovaccine, LPMan-GPC3/CL097...
2018: American Journal of Translational Research
Jun Zhang, Qi-Lu Qiao, Xiao-Chao Guo, Jian-Xun Zhao
OBJECTIVE: This study aims to investigate the role of three-dimensional visualization technique in the diagnosis and treatment of progressive hilar cholangiocarcinoma. METHODS: From January 2014 to February 2017, a three-dimensional visualization model was set up in 23 patients with progressive hilar cholangiocarcinoma. The distributions and variations of the hepatic portal ducts were observed. The tumors were classified based on Bismuth classification. The simulation operation was performed and the operation plan was established...
2018: American Journal of Translational Research
Feng Zhou, Dahui Ma, Ming Ouyang, Guiqin Liu, Tianhui Zhu, Yun Yang
OBJECTIVE: Mesenchymal stem cells (MSCs) derived from nasal mucosa are featured by high division and differentiation capacity, with large nuclei, obvious nucleoli and weak cytoplasmic basophily. Imaging examination, typically CT scan, is the gold standard for the diagnosis of orbital fracture. METHODS: We isolated MSCs derived from goat nasal mucosa and built the calcification model so as to investigate the repair mechanism of nasal mucosa-derived MSCs in orbital fracture...
2018: American Journal of Translational Research
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