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American Journal of Translational Research

Li Zhou, Jianxu Liu, Dong Dong, Chunsheng Wei, Rui Wang
Imbalance of Aβ production and Aβ removal leads to Aβ accumulation. Aβ degrading enzyme (including neprilysin-NEP, endothelin converting enzyme-ECE) as a therapeutic strategy for lowering brain Aβ deposition has attracted increasing attention. In this study, we investigated alteration of age and region-dependent in APP/PS1 double transgenic mice (3, 6, 9, 12 months) and their age-matched wild type mice including the ability of spatial memory, Aβ deposits, the protein expression, location and activity of NEP and ECE...
2017: American Journal of Translational Research
Song Li, Zhongchen Song, Jiachen Dong, Rong Shu
Tumor necrosis factor-alpha (TNF-α) has been shown to cause apoptosis of gingival epithelial cells (GECs) in periodontitis. However, the underlying molecular mechanism is still unclear. In this study, we showed that miR-142 expression was significantly elevated in human GECs after exposure to TNF-α. Such induction was in a time- and concentration-dependent manner. Serum miR-142 levels were positively correlated with serum TNF-α levels in patients with chronic periodontitis (r = 0.314, P = 0.0152). Depletion of miR-142 was found to attenuate TNF-α-induced apoptosis, as determined by TUNEL staining and caspase-3 activity assays...
2017: American Journal of Translational Research
Xi Shi, Baoqing Tian, Chi Ma, Lingxia Liu, Na Zhang, Yuan Na, Jing Li, Jun Lu, Yuehua Qiao
Cellular senescence is an irreversible form of cell cycle arrest, which is often characterized by domains of facultative heterochromatin substructures also known as senescence-associated heterochromatin foci (SAHF). SAHF assembly is likely mediated through the downregulation of the Wnt pathway, which inhibits Glycogen Synthase Kinase 3 Beta (GSK3β) in cells undergoing replicative senescence. Alternatively, High Mobility Group AT-Hook 2 (HMGA2) can also induce SAHF formation in primary cells, through a process in which the involved cell signaling pathway is unknown...
2017: American Journal of Translational Research
Xu-Ying Wan, Xiao-Feng Zhai, Yi-Ping Jiang, Ting Han, Qiao-Yan Zhang, Hai-Liang Xin
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related mortality worldwide. Norcantharidin (NCTD), a demethylated analog of cantharidin, possesses antimetastatic effects on HCC cells. The aim of this study was to identify target proteins of NCTD. In this study, we confirmed the antimetastatic effects of NCTD on SMMC-7721 and MHCC-97H cells. Through RNA sequencing, we found a non-canonical poly (A) polymerase, Family-with-sequence-similarity-46C (FAM46C) was up-regulated in response to NCTD exposure...
2017: American Journal of Translational Research
Jun Li, Juan Wei, Zhu Mei, Yongmei Yin, Yongfei Li, Mingjie Lu, Shidai Jin
Recent studies show that many microRNAs (miRNAs) are found to play important roles in breast cancer, however, most of miRNAs are not investigated completely. In the present study, significant down-regulation of miR-520a-3p was found in the breast cancer tissues and cell lines. The restoration of miR-520a-3p expression in breast cancer cells could inhibit cell proliferation, migration and invasion. In addition, miR-520a-3p was able to induce breast cancer cell apoptosis. Luciferase assay was used to confirm that CCND1 and CD44 were the direct target genes of miR-520a-3p...
2017: American Journal of Translational Research
Yanjie Zhang, Xiaohan Huang, Yanhao Yuan
BACKGROUND: Chondrogenic differentiation of mesenchymal stem cells (MSCs) is important for osteoarthritis (OA) treatment. However, the specific mechanisms involved are undefined. MicroRNAs (miRNAs) downregulate protein synthesis by binding to the 3'UTR of target mRNA. METHODS: Bone marrow aspirates were obtained from OA patients undergoing total hip arthroplasty (n=8) to isolate MSCs. MiR-410 or miR-410 inhibitor were transfected into MSCs using lentivirus and the effects were assessed...
2017: American Journal of Translational Research
Yinquan Zhang, Yulei Gao, Lijun Cai, Fengning Li, Yi Lou, Ning Xu, Yifan Kang, Huilin Yang
INTRODUCTION: MicroRNAs (miRNAs) has emerged as important factors in osteogenesis and chondrogenesis. This study aimed to determine whether miR-221 is involved in the regulation of osteoporosis and its underlying mechanism. METHODS: Total RNA was extracted from fresh femoral neck trabecular bone from women undergoing hip replacement due to either osteoporotic fracture (OP group, n = 12) or osteoarthritis in the absence of osteoporosis (Control group, n = 12). Gene expression was quantified using TaqMan quantitative RT-PCR assays and protein production was determined by western blot analysis...
2017: American Journal of Translational Research
Sen Zhang, Dongjie Wang, Nina Xue, Fangfang Lai, Ming Ji, Jing Jin, Xiaoguang Chen
Nicousamide, a clinical phase II renal protective new drug, has been demonstrated to have renal protective effect on diabetic nephropathy (DN) by experimental animal model. Its known molecular mechanisms include AGE formation blocking and moderately decreasing the blood pressure. Nicousamide shows potential on attenuating albuminuria, thereby suggests it might have protective effect on podocytes. The aim of present study was to investigate whether nicousamide could protect integrity of podocytes, and further its protection mechanisms...
2017: American Journal of Translational Research
Ting-Yi Sun, Hong-Jian Xie, Zhen Li, Ling-Fei Kong, Xiang-Nan Gou, Du-Juan Li, Yu-Jie Shi, Yan-Zhi Ding
miR-34a is an important molecule that can inhibit the tumor growth. This study aimed to investigate the functional role of miR-34a in hepatocellular carcinoma (HCC) and explore the interaction between miR-34a and histone deacetylase 1 (HDAC1). RT-qPCR was employed to detect the mRNA expression of miR-34a and HDAC1 in 60 HCC tissues. Results showed miR-34a expression in HCC tissues was significantly lower than in normal tissues (P<0.05), but HDAC1 expression in HCC tissues was markedly higher than in normal tissues (P<0...
2017: American Journal of Translational Research
Xiangjun Chen, Hao Dong, Sha Liu, Li Yu, Dexiong Yan, Xingwei Yao, Weijing Sun, Dezhi Han, Guozhen Gao
Increasing evidences demonstrated that long noncoding RNAs (lncRNAs) are frequently dysregulated and have critical roles in many tumors. However, the roles and functional mechanisms of lncRNAs in melanoma remain largely unknown. In this study, we identified a novel lncRNA MHENCR which was upregulated in melanoma tissues and further upregulated in metastatic melanoma. Increased expression of MHENCR indicted poor survival of melanoma patients. Functional experiments revealed that MHENCR knockdown significantly inhibited melanoma cells proliferation, induced cell cycle arrest and apoptosis, and also attenuated melanoma cells migration in vitro...
2017: American Journal of Translational Research
Jinquan Hu, Qiang Yan, Changgui Shi, Ye Tian, Peng Cao, Wen Yuan
We previously showed that bone mesenchymal stem cells (BMSCs) inhibit interleukin-1 beta (IL-1β) induced degenerative effects in NP cells by their paracrine activity, but the anti-inflammatory and anti-apoptotic effect of BMSC paracrine activity and the relative signaling pathway were not further investigated in annulus fibrosus (AF) cells. In this study, AF cells were exposed to IL-1β, which was applied to mimic intervertebral disc degeneration (IDD) in vitro. Indirect co-culture with BMSCs in a transwell co-culture system reduced the activity of nuclear factor-κB-p65 (NF-κB-p65) through the restoration of its inhibitor IκBa...
2017: American Journal of Translational Research
Xin Long, Qi Cheng, Huifang Liang, Jianping Zhao, Jian Wang, Wei Wang, Stephen Tomlinson, Lin Chen, Carl Atkinson, Bixiang Zhang, Xiaoping Chen, Peng Zhu
BACKGROUND: Acute graft rejection mediated by alloreactive memory CD4(+) T cells is a major obstacle to transplantation tolerance. It has been reported that CD8(+) T regulatory cells (Tregs) have the ability to induce graft tolerance by restraining the function of activated CD4(+) T cells, but not including memory T cells. The aim of this study is to elucidate the effect of CD8(+) Tregs on alloreactive memory CD4(+) T cells. METHODS: We detected Qa-1 expression and performed proliferative assay on memory CD4(+) T cells...
2017: American Journal of Translational Research
Xiaohuan Zhang, Sainan Zhao, Luping Sun, Wenqing Li, Qiao Wei, Robert B Ashman, Yan Hu
Candida albicans is an important opportunistic fungus causing both disseminated and local infections. The discovery of neutrophil extracellular traps (NETs) has presented a new strategy to kill microorganisms in host's innate immune response. Although it has been reported that NETs can trap and kill both yeast and hyphal forms of C. albicans, the mechanism by which C. albicans escape from NETs has not been fully understood. In this study, the ability of two strains of C. albicans SC5314 and 3683 to escape NETs-mediated killing was compared...
2017: American Journal of Translational Research
Hoa Thi Thai Pham, Wansu Huang, Chuangye Han, Juman Li, Qiuqiao Xie, Jinbin Wei, Xiaohui Xu, Zefeng Lai, Xiang Huang, Renbin Huang, Qingwei Wen
Recently, many reports have shown that Averrhoa carambola L. (Oxalidaceae) juice (EACJ) could reduce blood glucose in humans. However, its mechanisms have not been well explored; therefore, our study aimed to investigate the beneficial effects of EACJ on hyperglycemia, hyperlipidemia and renal injury in streptozotocin (STZ)-induced diabetic mice. Those mice were injected with STZ via the tail vein (120 mg/kg body weight) and were identified as diabetic mice when the level of blood glucose was ≥ 11.1 mmol/L...
2017: American Journal of Translational Research
Mohd Abul Kalam, Abdul Arif Khan, Aws Alshamsan
Polymeric nanoparticulate carriers play an important role and holding a significant potential for the development of novel immunomodulatory agents as easily they are taken up by antigen presenting cells. They allow an enhanced antigen stability, better immunogenicity and immunostimulatory effect with sustained and controlled release of the antigen to the target sites. Better information and vital understanding of mechanism of action, interaction of such vectors with the APCs and dendritic cells and antigen release kinetics in immunomodulatory effects, and improved knowledge of their in vivo fate and distribution are now needed, those collectively would speed up the rational strategies of nanoparticles as carriers for vaccines and other protein antigens...
2017: American Journal of Translational Research
Lei Huang, A-Man Xu
Lysine methylation plays a vital role in histone modification. Deregulations of lysine methyltransferases and demethylases have been frequently observed in human cancers. The SET and MYND domain containing protein 3 (SMYD3) is a novel histone lysine methyltransferase and it functions by regulating chromatin during the development of myocardial and skeletal muscle. It has been recently unveiled to play significant roles in human cancer genesis and progression via regulating various key cancer-associated genes and pathways and promoting cell proliferation and migration...
2017: American Journal of Translational Research
Xiao-Fei Li, Yan Wang, Dong-Dong Zheng, Hai-Xia Xu, Teng Wang, Min Pan, Jia-Hai Shi, Jian-Hua Zhu
OBJECTIVE: The identification of the biological function of M1 macrophages and the mechanism underlying their role in valvular interstitial cell (VIC) calcification may provide therapeutic targets for the prevention of aortic valve calcification (AVC). This study investigated the mechanism by which M1 macrophages and macrophage-derived microvesicles (MVs) affected the calcification of VICs. An additional aim was to investigate the involvement of the miR-214 pathway in this process. METHODS: The M1 or M2 macrophage phenotype in human calcific aortic valve was confirmed by gene expression analysis of M1 or M2 macrophage markers...
2016: American Journal of Translational Research
Xiang-Qian Luo, Shao-Bo Yang, Shu-Qi Qiu, Rui-Di Xie, Li-Tao Yang, Yu-Xing Ke, Hong-Xia Zhao, Xiao-Rui Geng, Gui Yang, Zhi-Qiang Liu, Jiang-Qi Liu, Shuai Wang, Da-Bo Liu, Jun Liu
The dysfunction of peripheral immune tolerance plays an important role in the pathogenesis of allergic diseases. Recent reports indicate that micro RNA (miR)-98 is associated with the process of aberrant immune responses. This study aims to test a hypothesis that miR-98 is associated with the pathogenesis of airway allergy via interfering with the development of regulatory B cells (Breg). In this study, patients with airway allergy were recruited into this study. The frequency of Bregs was assessed by flow cytometry...
2016: American Journal of Translational Research
Patrycja Sosińska, Ewa Baum, Beata Maćkowiak, Ryszard Staniszewski, Tomasz Jasinski, Kazuo Umezawa, Andrzej Bręborowicz
Peritoneal mesothelial cells exposed to bioincompatible dialysis fluids contribute to damage of the peritoneum during chronic dialysis. Inflammatory response triggered in the mesothelium leading to neovascularization and fibrosis plays an important role in that process. We studied the effects of Dehydroxymethyepoxyquinmicin (DHMEQ)-an NF-κB inhibitor on function of human peritoneal mesothelial cells (HPMC) in in vitro culture. DHMEQ studied in concentrations of 1-10 µg/ml was not toxic to HPMC. Synthesis of IL-6, MCP-1 and hyaluronan in unstimulated and stimulated with interleukin-1 (100 pg/ml) HPMC was inhibited in the presence of DHMEQ and the effect was proportional to the dose of the drug...
2016: American Journal of Translational Research
Jiapeng Liu, Wei Dong, Tao Wang, Liang Liu, Long Zhan, Yifei Shi, Jiange Han
OBJECTIVE: To investigate the effects of etomidate and propofol on immune function in patients with lung adenocarcinoma. METHODS: Sixty patients who were scheduled for lung cancer surgery under general anesthesia were studied. The patients were randomly divided into an etomidate total intravenous anesthesia group (group E) and a propofol total intravenous anesthesia group (group P), with 30 cases in each group. RESULTS: Within group comparison: The percentage of CD4+ in the two groups was significantly reduced at 24 hours post-operation (T2) compared with the percentage before surgery, whereas the percentage of CD8+ was higher at T2...
2016: American Journal of Translational Research
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