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American Journal of Translational Research

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https://www.readbyqxmd.com/read/28469808/methylation-of-kruppel-like-factor-2-klf2-associates-with-its-expression-and-non-small-cell-lung-cancer-progression
#1
Wenbin Jiang, Xueqing Xu, Shaoli Deng, Jie Luo, Huan Xu, Chao Wang, Tingting Sun, Guoqin Lei, Fengling Zhang, Cheng Yang, Lin Zhou, Feng Wang, Ming Chen
Kruppel-like factor 2 (KLF2) is a putative tumor suppressor gene. This study investigated its role and epigenetic mechanisms in human non-small cell lung cancer (NSCLC) in ex vivo and in vitro. A total of 47 paired NSCLC and normal tissues and six cell lines were analyzed using qRT-PCR for KLF2 expression. KLF2 methylation was assessed using the methylation specific PCR (MSP) or bisulfite sequencing PCR (BSP). Functional KLF2 region 4 (+567 to +906) was confirmed using the dual-luciferase reporter assay, while CCK-8 cell viability and flow cytometric assays were used to assess changes in cell viability, cell cycle distribution, and apoptosis after knockdown or re-expression of KLF2...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469807/protein-bound-p-cresol-inhibits-human-umbilical-vein-endothelial-cell-proliferation-by-inducing-cell-cycle-arrest-at-g0-g1
#2
Li Li, Jing Li, Xun Li, Fa-Huan Yuan
P-cresol is a typical protein-bound uremic toxin, which is retained in patients with renal failure. It is not known whether protein-bound P-cresol exhibits the toxicity in humans. This study aims to investigate the endothelial toxicity of protein-bound P-cresol. Cultured human umbilical vein endothelial cells (HUVEC) were treated with unbound or human serum albumin-bound (HSA, 4 g/dL), P-cresol (0, 20, 40, 80 μg/mL) for 24, 48, 72 h, respectively. Cell viability was determined by using cell counting kit-8 (CCK-8) assay...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469806/radial-extracorporeal-shock-wave-therapy-improves-cerebral-blood-flow-and-neurological-function-in-a-rat-model-of-cerebral-ischemia
#3
Nan Kang, Jing Zhang, Xiaotong Yu, Yuewen Ma
We performed middle cerebral artery occlusion (MCAO) in rats to investigate the effect and some of the underlying mechanisms of radial extracorporeal shock wave therapy (rESWT) in cerebral ischemia rats. We measured neurological function and cerebral blood flow (CBF) using a full-field laser perfusion imager and brain infarct volume on days 3, 12, and 30. Immunofluorescence, western blot, and real-time polymerase chain reaction (PCR) techniques were used to detect the expression of vascular endothelial growth factor (VEGF), neuron-specific enolase (NSE), nestin, Wnt3a, and β-catenin in the ischemic hemisphere...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469805/therapeutic-ultrasound-protects-huvecs-from-ischemia-hypoxia-induced-apoptosis-via-the-pi3k-akt-pathway
#4
Jing-Juan Huang, Yi-Qin Shi, Rui-Lin Li, An Hu, Zhao-Yang Lu, Liang Weng, Yi-Peng Han, Shen-Qi Wang, Lan Zhang, Chang-Ning Hao, Jun-Li Duan
Background: Previous studies have demonstrated that therapeutic ultrasound (TUS) ameliorates angiogenesis on ischemic hind limb animals and also promotes human umbilical vein endothelial cells (HUVECs) tube formation. Apoptosis plays a key role in post-ischemic angiogenesis pathogenesis. However, the mechanisms underlying the anti-apoptotic effects of TUS are not clear. Therefore we put forward the hypothesis that TUS might promote angiogenesis during ischemia/hypoxia (I/H) by decreasing apoptosis. Methods: We investigated the cytoprotective role of TUS and the underlying mechanisms in I/H-induced HUVEC apoptosis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469804/long-noncoding-rna-crnde-activates-wnt-%C3%AE-catenin-signaling-pathway-through-acting-as-a-molecular-sponge-of-microrna-136-in-human-breast-cancer
#5
Jinliang Huan, Li Xing, Qianhuang Lin, Hu Xui, Xianju Qin
Long non-coding RNAs (lncRNAs) serve critical roles in the tumorigenesis and development of multiple human malignancies. Herein, we aimed to explore the biological and clinical significance of lncRNA CRNDE in human breast cancer (BC). The expression of CRNDE in BC tissues and cell lines was detected, and the association between CRNDE expression and clinicopathologic features of BC patients was also analyzed. Novel targets of CRNDE were identified through a bioinformatics search and confirmed using a dual-luciferase reporter system...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469803/construction-and-analysis-of-the-transcription-factor-microrna-co-regulatory-network-response-to-mycobacterium-tuberculosis-a-view-from-the-blood
#6
Yan Lin, Zipeng Duan, Feng Xu, Jiayuan Zhang, Marina V Shulgina, Fan Li
Mycobacterium tuberculosis (Mtb) infection has been regional outbreak, recently. The traditional focus on the patterns of "reductionism" which was associated with single molecular changes has been unable to meet the demand of early diagnosis and clinical application when current tuberculosis infection happened. In this study, we employed a systems biology approach to collect large microarray data sets including mRNAs and microRNAs (miRNAs) to identify the differentially expressed mRNAs and miRNAs in the whole blood of TB patients...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469802/erratum-differential-expression-of-mir-21-and-mir-75-in-esophageal-carcinoma-patients-and-its-clinical-implication
#7
Guoyu Huang
[This corrects the article on p. 3288 in vol. 8, PMID: 27508050.].
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469801/erratum-mir-4295-promotes-cell-growth-in-bladder-cancer-by-targeting-btg1
#8
Yong-Hao Nan, Jun Wang, Yao Wang, Peng-Hao Sun, Yu-Ping Han, Li Fan, Kai-Chen Wang, Fu-Jun Shen, Wei-Hua Wang
[This corrects the article on p. 4892 in vol. 8, PMID: 27904689.].
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469800/erythema-elevatum-diutinum-involving-palms-and-soles-a-case-report-and-literature-review
#9
Uma Keyal, Anil Kumar Bhatta, Yeqiang Liu
Erythema elevatum diutinum (EED) is a rare chronic inflammatory dermatosis and a part of the spectrum of cutaneous leukocytoclasticvasculitis. The most common site of involvement is extensor surface of the extremities, with a predilection for the skin overlying joints, particularly hands, feet, elbows and knees, as well as buttocks and Achilles tendons. Here we report a case of EED with atypical presentation involving palms and soles. The patient showed dramatic response to the treatment with prednisolone combined with Tripterygium wilfordii glycoside (TWP)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469799/histone-h3k14-hypoacetylation-and-h3k27-hypermethylation-along-with-hdac1-up-regulation-and-kdm6b-down-regulation-are-associated-with-active-pulmonary-tuberculosis-disease
#10
Yung-Che Chen, Tung-Ying Chao, Sum-Yee Leung, Chung-Jen Chen, Chao-Chien Wu, Wen-Feng Fang, Yi-Hsi Wang, Huang-Chih Chang, Ting-Ya Wang, Yong-Yong Lin, Yi-Xin Zheng, Meng-Chih Lin, Chang-Chun Hsiao
The aim of this study is to determine the roles of global histone acetylation (Ac)/methylation (me), their modifying enzymes, and gene-specific histone enrichment in active pulmonary tuberculosis (TB) disease. Global histone H3K27me3, H3K27me2, H3K9me3, H3K9Ac, and H3K14Ac expressions, and their modifying enzyme expressions, including KDM1A, KDM6B, EZH2, HDAC1, and HDAC2, were assessed in blood leukocytes from 81 patients with active pulmonary TB disease and 44 matched healthy subjects (HS). TLR2, TNF-α, IFN-γ, and IL12B-specific histone enrichment of peripheral blood mononuclear cells was measured by chromatin immunoprecipitation method...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469798/inhibition-of-autophagy-results-in-a-reversal-of-taxol-resistance-in-nasopharyngeal-carcinoma-by-enhancing-taxol-induced-caspase-dependent-apoptosis
#11
Yexun Song, Wei Li, Xiaowei Peng, Jun Xie, Heqing Li, Guolin Tan
Drug resistance in nasopharyngeal carcinoma remains a major obstacle of clinical therapy. We found that the taxol-resistant cells demonstrated higher basal levels of autophagy than parental cells, which could be inhibited by 3-MA and Beclin-1-siRNA. We further revealed that inhibition of autophagy enhanced taxol-induced caspase-dependent apoptosis, resulted in partial reversal of the acquired taxol resistance in taxol-resistant cells. Our results suggest that the combination of an autophagy inhibitor with taxol may be a promising approach to promote therapeutic efficacy in patients with nasopharyngeal carcinoma...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469797/downregulation-of-p38-phosphorylation-correlates-with-low-grade-differentiation-and-proliferation-of-lung-squamous-cell-carcinoma
#12
Yandong Nan, Ruijing Chang, Hua Jiang, Shuanying Yang, Faguang Jin, Yonghong Xie
BACKGROUND: P38MAPK has been investigated as a tumor-related signaling molecule because of its apparent association with tumorigenesis. This study aimed to investigate P38MAPK expression and its role in lung squamous carcinoma (LSCC). METHODS: The expression of P38MAPK and phosphorylated P38 (P-P38) in LSCC tissues and cells was examined by Western blot, real-time PCR, and immunohistochemistry. The influence of P38MAPK inhibitor SB203580 on the proliferation of LSCC cells was detected by MTT and flow cytometry...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469796/low-dose-radiation-modulates-human-mesenchymal-stem-cell-proliferation-through-regulating-cdk-and-rb
#13
Lei Yang, Ziling Liu, Chen Chen, Xiaofeng Cong, Zhi Li, Shasha Zhao, Meng Ren
Low-dose radiation (LDR) has been known to stimulate cell proliferation. The effect of LDR on human bone marrow mesenchymal stem cells (BMSCs), however, remains to be determined. The current study, therefore, aimed to investigate the effect of LDR on human BMSC proliferation and its mechanisms. To accomplish this, human BMSCs were isolated from ribs and cultured with or without exposition to LDR (75 mGy) for 24 h. Cell proliferation was assessed by MTT assay, the cytokines secreted by the BMSCs were quantified by ELISA, and the proteins associated with cell proliferation and cell cycle were evaluated by immunoblot analysis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469795/microrna-199a-3p-attenuates-hepatic-lipogenesis-by-targeting-sp1
#14
Yang Cheng, Li Huang, Jian Ping, Tianyang Chen, Jianjie Chen
Emerging studies have demonstrated that microRNAs (miRs) are profoundly involved in non-alcoholic fatty liver disease (NAFLD) and related metabolic diseases. Previously, we revealed a repertoire of miRs dysregulated in NAFLD by high-throughput sequencing. Here, we showed that microRNA-199a-3p was down-regulated in the livers of C57BL/6J mice fed a high-fat-diet (HFD) and oleic acid/palmitic acid-induced Hepa1-6 cells. Gain-of-function and loss-of-function studies demonstrated that microRNA-199a-3p exhibited a suppressive role in hepatic lipogenesis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469794/silencing-of-cxcr4-and-cxcr7-expression-by-rna-interference-suppresses-human-endometrial-carcinoma-growth-in-vivo
#15
Yu Huang, Yuanying Ye, Ping Long, Shuping Zhao, Lei Zhang, Yanni A
In this paper, the effect of silencing the expression of CXCR4 and CXCR7 by RNAi on the growth of endometrial carcinoma (EC), in vivo, was evaluated. To establish endometrial carcinoma model, thirty nude mice were subcutaneously inoculated with 1 × 10(7) Ishikawa cells. All tumor-bearing mice were randomly assigned to five groups (six mice in each group) when the tumor xenografts reached 5-7 mm in diameter, and treated with CXCR4-siRNA (5 nmol), CXCR7-siRNA (5 nmol), CXCR4-siRNA (5 nmol) plus CXCR7-siRNA (5 nmol), negative-siRNA (5 nmol) and normal saline, respectively...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469793/downregulation-of-mir-301a-3p-sensitizes-pancreatic-cancer-cells-to-gemcitabine-treatment-via-pten
#16
Xiang Xia, Kundong Zhang, Guangtao Luo, Gang Cen, Jun Cao, Kejian Huang, Zhengjun Qiu
BACKGROUND: We previously showed that miR-301a-3p affects the invasion and migration abilities of pancreatic cancer cells. Here, we explore the role of miR-301a-3p in chemoresistance, which represents a major obstacle in cancer treatment. METHODS: We tested the effects of miR-301a-3p ongemcitabine resistance in cytotoxicity assays in vitro and in vivo. We used quantitative real-time PCR (qRT-PCR) to measure miR-301a-3p expression in wild-type and gemcitabine-resistant pancreatic cancer cells...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469792/large-conductance-voltage-and-ca-2-activated-k-channels-affect-the-physiological-characteristics-of-human-urine-derived-stem-cells
#17
Qingqing Wang, Jiang Zhao, Chao Wu, Zhenxing Yang, Xingyou Dong, Qian Liu, Bishao Sun, Chen Wei, Xiaoyan Hu, Longkun Li
We investigated the current characteristics of large conductance voltage and Ca(2+)-activated K(+) (BK) channels in human urine-derived stem cells (hUSCs) and the effect of BK channels on proliferation and differentiation of hUSCs. Fresh human urine (n=6) was collected from healthy donors to isolate hUSCs. Human KCNMA1 gene silencing U6 shRNA was used to down regulate the expression of BK in hUSCs. IBTX (BK channel antagonist) and NS1619 (BK channel agonist) were used to examine the effect of BK channels on hUSCs...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469791/curcumin-up-regulates-t-helper-1-cells-in-patients-with-colon-cancer
#18
Bin Xu, Lin Yu, Li-Zhong Zhao
The therapy for the advanced colon cancer (Cca) is unsatisfactory currently. To regulate the immune effector cell function has shown a positive effect on the treatment of advanced cancers. This study tests a hypothesis that administration with curcumin converts the Cca patient-derived regulatory T cells (Treg) to T helper (Th) 1 cells. In this study, a group of patients with advanced Cca was recruited into this study. The patients were treated with curcumin. The peripheral Tregs and Th1 cells were assessed by flow cytometry...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469790/mir-130-exerts-tumor-suppressive-function-on-the-tumorigenesis-of-human-non-small-cell-lung-cancer-by-targeting-pten
#19
Ling Ye, Yiming Wang, Lin Nie, Shen Qian, Meng Xu
MicroRNAs (miRNAs) have been involved in some human malignancies and correlated with tumor progression. The dysregulation of miR-130 is found in various cancers and correlated with tumor proliferation and apoptosis. However, its expression and function in non-small cell lung cancer (NSCLC) have not been investigated yet. In this study, we demonstrated that miR-130 is significantly down-regulated in NSCLC tissue samples and cell lines. Low miR-130 expression was closely associated with lymph node metastasis, late stages of disease progression and diminished survival in NSCLC patients...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28469789/long-non-coding-rna-xist-exerts-oncogenic-functions-in-human-glioma-by-targeting-mir-137
#20
Zong Wang, Jiangwei Yuan, Li Li, Yang Yang, Xuchang Xu, Ying Wang
Long non-coding RNA (lncRNA) X inactivate-specific transcript (XIST) acts as an important regulator in tumor progression. However, its expression and the underlying mechanism in glioma remain unclear. The aim of this study was to explore the potential function of XIST in glioma progression. In the present study, our data showed that the expression of XIST was significantly up-regulated in glioma tissues and enhanced the proliferation of glioma cells. The expression of miR-137 was significantly decreased in glioma tissues...
2017: American Journal of Translational Research
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