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American Journal of Translational Research

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https://www.readbyqxmd.com/read/28386378/transplantation-of-multipotent-isl1-cardiac-progenitor-cells-preserves-infarcted-heart-function-in-mice
#1
Yunpeng Li, Shuo Tian, Ienglam Lei, Liu Liu, Peter Ma, Zhong Wang
Cell-based cardiac therapy is a promising therapeutic strategy to restore heart function after myocardial infarction (MI). However, the cell type selection and ensuing effects remain controversial. Here, we intramyocardially injected Isl1+ cardiac progenitor cells (CPCs) derived from EGFP/luciferase double-tagged mouse embryonic stem (dt-mES) cells with vehicle (fibrin gel) or phosphate-buffered saline (PBS) into the infarcted area in nude mice to assess the contribution of CPCs to the recovery of cardiac function post-MI...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386377/hypoxia-inducible-factor-1%C3%AE-promotes-survival-of-mesenchymal-stem-cells-under-hypoxia
#2
Bingke Lv, Feng Li, Jie Fang, Limin Xu, Chengmei Sun, Jianbang Han, Tian Hua, Zhongfei Zhang, Zhiming Feng, Xiaodan Jiang
Mesenchymal stem cells (MSCs) are ideal materials for cell therapy. Research has indicated that hypoxia benefits MSC survival, but little is known about the underlying mechanism. This study aims to uncover potential mechanisms involving hypoxia inducible factor 1α (HIF1A) to explain the promoted MSC survival under hypoxia. MSCs were obtained from Sprague-Dawley rats and cultured under normoxia or hypoxia condition. The overexpression vector or small interfering RNA of Hif1a gene was transfected to MSCs, after which cell viability, apoptosis and expression of HIF1A were analyzed by MTT assay, flow cytometry, qRT-PCR and Western blot...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386376/long-non-coding-rna-gas5-inhibits-tumorigenesis-via-mir-137-in-melanoma
#3
Donghui Bian, Wen Shi, Yang Shao, Peilong Li, Guodong Song
Melanoma is the leading cause of death in patients with skin cancer. In the present study, we aimed to prove the functions and molecular mechanisms of lncRNA-GAS5 in melanoma. Herein, we found that the expression of GAS5 was down-regulated in melanoma tissues compared to adjacent normal tissues. GAS5 was significantly associated with distal metastasis and TNM stage in melanoma. Furthermore, we found that GAS5 suppressed melanoma cell proliferation, migration and invasion. Then, we found thatmiR-137 was decreased in melanoma tissues compared to adjacent normal tissues and was correlated with GAS5...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386375/expression-of-antisense-of-microrna-26a-5p-in-mesenchymal-stem-cells-increases-their-therapeutic-effects-against-cirrhosis
#4
Li Chen, Wenhuan Zeng, Bo Yang, Xiang Cui, Cong Feng, Lili Wang, Hao Wang, Xuan Zhou, Peng Li, Faqin Lv, Tanshi Li
Hepatocyte growth factor (HGF) is a potent mitogen for mature hepatocytes, and has been shown to prevent cirrhosis during liver regeneration. Transplantation of mesenchymal stem cells (MSCs) reduces the development of cirrhosis after liver injury. However, the production and secretion of transplanted MSCs in liver were not studied yet. Here we found that the MSCs expressed low levels of HGF protein, but surprisingly high levels of HGF mRNA. Further investigation using bioinformatics analyses and luciferase reporter assay showed that MSCs expressed high levels of microRNA-26a-5p (miR-26a-5p), which targeted 3'-UTR of HGF mRNA to inhibit its protein translation...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386374/microrna-137-inhibits-growth-of-glioblastoma-through-egfr-suppression
#5
Zhenxing Zhang, Xiaofeng Song, He Tian, Ye Miao, Xu Feng, Yang Li, Honglei Wang
Aberrant expression of certain microRNAs (miRNAs) has been shown to contribute to the development of Glioblastoma multiforme (GBM). However, the involvement of miR-137 in the carcinogenesis of GBM has not been reported. Here, we showed that miR-137 levels in GBM tissues were significantly lower than the paired normal brain tissue in patients' specimens. Moreover, low miR-137 levels in GBM tissue were associated with poor prognosis. In vitro, overexpression of miR-137 decreased GBM cell growth and increased cell apoptosis, while depletion of miR-137 enhanced cell growth and decreased cell apoptosis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386373/mechanism-of-progestin-resistance-in-endometrial-precancer-cancer-through-nrf2-survivin-pathway
#6
Rujia Fan, Yiying Wang, Yue Wang, Li Wei, Wenxin Zheng
Progestin is commonly used for young patients suffering from endometrial hyperplasia or cancer. However, there is approximately 30% failure rate with unclear mechanism. We investigated if Nrf2-survivin pathway contributes the progestin resistance (PR) in this setting. Current study detected Nrf2 and survivin protein expression in post progestin treated endometrial tissue samples by using immunohistochemistry. Transfection of Nrf2 and survivin into endometrial cancer cells in vitro was done to determine the roles of Nrf2 and survivin in progestin resistance...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386372/thioredoxin-2-protects-against-oxygen-glucose-deprivation-reperfusion-injury-by-inhibiting-autophagy-and-apoptosis-in-h9c2-cardiomyocytes
#7
Yan-Yan Li, Yin Xiang, Song Zhang, Yan Wang, Jie Yang, Wei Liu, Feng-Tai Xue
The aim of this study is to examine the role of thioredoxin-2 (Trx2) in autophagy and apoptosis during myocardial ischemia-reperfusion (I/R) injury in vitro. We employed the oxygen-glucose deprivation and reperfusion (OGD/R) model of H9c2 cells and used lentiviral infection to overexpress Trx2. H9c2 cell viability and injury assays were conducted using a Cell Counting Kit-8 (CCK-8) and alactate dehydrogenase (LDH) kit. The effects of Trx2 on autophagy and apoptosis were measured by transmission electron microscopy (TEM), western blot, and flow cytometry...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386371/immunomodulatory-activity-of-interleukin-27-in-human-chronic-periapical-diseases
#8
Juan Li, Rong Wang, Shi-Guang Huang
This study aims to observe expression of IL-27 on different cells in periapical tissues of different types of human chronic periapical diseases. Periapical tissue specimens of 60 donors, including healthy control (n=20), periapical granuloma group (n=20) and radicular cysts group (n=20), were fixed in 10% buffered formalin, stained with hematoxylin and eosin for histopathology. Then specimens were stained with double- immuno-fluorescence assay for identification of IL-27-tryptase (mast cells, MCs), IL-27-CD14 (mononuclear phagocyte cells, MPs) and IL-27-CD31 (endothelial cells, ECs) double-positive cells in periapical tissues...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386370/modifications-of-anxiety-like-behavior-in-prenatally-stressed-male-offspring-with-imbalance-of-androgens
#9
Julia Fedotova, Viktoria Akulova, Svetlana Pivina, Jozef Dragasek, Martin Caprnda, Peter Kruzliak
Gonadal hormones have been well-known to affect brain regions known to be involved in the modulation of mood and affective-related behavior. Prenatal stress might alter hypothalamic-pituitary-gonadal axis, it could be a target for development of affective-related disorders in male offspring. The present study was designed to examine an anxiety-like behavior in the adult male offspring with low levels of endogenous androgens delivered from pregnant dams exposed to prenatal stress from gestation day 15 to gestation day 19...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386369/prognostic-role-of-lymphatic-vessel-density-and-lymphovascular-invasion-in-chemotherapy-naive-and-chemotherapy-treated-patients-with-invasive-breast-cancer
#10
Joanna A Niemiec, Agnieszka Adamczyk, Aleksandra Ambicka, Anna Mucha-Małecka, Wojciech M Wysocki, Beata Biesaga, Marek Ziobro, Ida Cedrych, Aleksandra Grela-Wojewoda, Małgorzata Domagała-Haduch, Joanna Wysocka, Janusz Ryś, Beata Sas-Korczyńska
It is assumed that the spread of breast cancer cells via the lymphatic system might be influenced by inflammatory reactions and/or the application of chemotherapy or molecularly targeted therapy. Therefore, we analysed survival according to lymphatic vessel density (LVD), lymphovascular invasion (LVI) (both assessed using podoplanin as immunohistochemical marker of lymphatic endothelium) and well-established clinico-pathological features in a group of 358 patients with invasive ductal breast cancer: 139 chemotherapy-naïve (pT1-2/pN0/M0) and 219 treated with chemotherapy (pT1-4/pN1-3/M0)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386368/diffusional-kurtosis-imaging-in-evaluating-the-secondary-change-of-corticospinal-tract-after-unilateral-cerebral-infarction
#11
Shun Zhang, Wenjie Zhu, Yan Zhang, Yihao Yao, Jingjing Shi, Cong-Yi Wang, Wenzhen Zhu
We investigated to evaluate whether diffusional kurtosis imaging (DKI) can early detect the microstructure change of corticospinal tract (CST) after unilateral cerebral infarction solely in middle cerebral artery (MCA) territory. Seventy-seven patients with MCA territory infarct consisting of 10 subjects of hyperacute phase, 22 subjects of acute phase, 28 subjects of subacute phase and 17 subjects of chronic phase were enrolled in this study. ROI method was performed to measure the mean value of the infarcted area and the areas which belongs to CST [including the posterior limb of internal capsule (PLIC), cerebral peduncle (CP), pons, and medulla] in both ipsilateral and contralateral mirror side in all the DKI-derived parametric maps...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386367/targeting-tgf-%C3%AE-1-suppresses-survival-of-and-invasion-by-anaplastic-thyroid-carcinoma-cells
#12
Wenhai Sun, Yanyan Xu, Cheng Zhao, Fengyun Hao, Dong Chen, Jinping Guan, Kejun Zhang
BACKGROUND AND AIMS: Overexpression of transforming growth factor (TGF)-β1 has been implicated in promoting cell survival, migration and invasion in many cancers, including anaplastic thyroid cancer (ATC). In the present study, we studied the effect of suppressing TGF-β1 by RNA silencing on the survival, invasion and metastasis of ATC cells. METHODS: Small interfering RNA (siRNA) constructs targeting TGF-β1 were validated and used to develop clonal derivatives of the ATC cell line, 8505C...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386366/micro-rna-19a-suppresses-interleukin-10-in-peripheral-b-cells-of-patients-with-diabetic-retinopathy
#13
Cong Wang, Qisheng You, Xusheng Cao, Huiling Guo, Xinxiao Gao, Xiaoyan Peng
A number of patients with diabetes suffer from retinopathy; the pathogenesis is to be further investigated. Recent reports indicate that micro RNA (miR) plays critical roles in the development of immune inflammation. This study test a hypothesis that miR-17-92 cluster is associated with the pathogenesis of diabetes retinopathy (DR). In this study, peripheral blood samples were collected from DR patients and healthy subjects. B cells were isolated from the blood samples to be analyzed the expression of interleukin (IL)-10...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386365/calpain-1-regulates-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-in-human-lung-epithelial-cells-via-pi3k-akt-signaling-pathway
#14
Wei-Jun Tan, Qiu-Yue Tan, Ting Wang, Min Lian, Li Zhang, Zhen-Shun Cheng
Cell proliferation, transformation, and epithelial-mesenchymal transition (EMT) are key processes involved in the development of idiopathic pulmonary fibrosis (IPF). This study investigated the regulatory factors and signaling pathways that mediate EMT in the human type II alveolar epithelial A549 cell line. A549 cells were cultured in RPMI-1640 medium and allocated to the following four groups: blank control group or treated with transforming growth factor-β1 (TGF-β1), TGF-β1 + PD 150606 (a calpain 1 inhibitor), or PD 150606...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386364/nestin-servers-as-a-promising-prognostic-biomarker-in-non-small-cell-lung-cancer
#15
Fang Liu, Yuan Zhang, Ming Lu, Cong Wang, Qingbao Li, Yongsheng Gao, Dianbin Mu, Yan Cao, Miaomiao Li, Xiangjiao Meng
Lung cancer is currently the leading cause of cancer-related death worldwide and it is important to identify the predictive and/or prognostic markers for the cancer. Nestin, a proliferative and multipotent biomarker has been reported to be associated with prognosis in non-small cell lung cancer (NSCLC) in a few studies. In the present study, we retrospectively recruited 153 patients with NSCLC. Nestin protein expression in tumor samples was determined by immunohistochemistry staining. Nestin expression was related with tumor differentiation (P=0...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386363/bioinformatics-and-co-expression-network-analysis-of-differentially-expressed-lncrnas-and-mrnas-in-hippocampus-of-app-ps1-transgenic-mice-with-alzheimer-disease
#16
Min Fang, Pei Zhang, Yanxin Zhao, Xueyuan Liu
APP/PS1 transgenic mice with Alzheimer disease (AD) are widely used as a reliable animal model in studies about behaviors, physiology, biochemistry and histomorphology of AD, but few studies have been conducted to investigate the role of lncRNAs in this model. In this study, lncRNA microarray was employed to detect the gene expression profile and lncRNA expression profile in the mouse brain. Then, bioinformatics was used to predict the differentially expressed genes related to AD (n=20). Among different lncRNAs (n=249), 99 were downregulated and 150 upregulated...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386362/b7-h3-regulates-migration-and-invasion-in-salivary-gland-adenoid-cystic-carcinoma-via-the-jak2-stat3-signaling-pathway
#17
Teng-Fei Fan, Wei-Wei Deng, Lin-Lin Bu, Tian-Fu Wu, Wen-Feng Zhang, Zhi-Jun Sun
B7 Homolog 3 (B7-H3), a newly identified member of the B7 family, is over-expressed in various human cancers and plays a vital role in tumor progression. To identify the expression pattern of B7-H3 in human salivary adenoid cystic carcinoma (AdCC) and its underlying mechanisms, we characterized B7-H3 expression in AdCC tissue microarrays using immunohistochemical staining, and analyzed potentially associated molecules. The results showed that B7-H3 was highly expressed in salivary AdCC, compared with normal salivary glands...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386361/downregulation-of-mir-429-contributes-to-the-development-of-drug-resistance-in-epithelial-ovarian-cancer-by-targeting-zeb1
#18
Jing Zou, Ling Liu, Qi Wang, Fuqiang Yin, Zhijun Yang, Wei Zhang, Li Li
Drug resistance is an obstacle to the treatment of epithelial ovarian cancer. Recently, research has suggested that miRNAs (microRNAs) are involved in cancer development, and speculation has been made about their possible involvement in drug resistance. Thus, we attempted to identify selected miRNAs involved in the development of chemo-resistance in epithelial ovarian cancer. Using miRNA profiles of a panel of cisplatin-resistant (SKOV3/DDP) cells, we validated data using quantitative real time-PCR (QRT-PCR), and studied the effects of miR-429 on cancer cell chemo-sensitivity, using gain- and loss-of-function studies...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386360/neuroprotective-effect-of-mir-665-against-sevoflurane-anesthesia-induced-cognitive-dysfunction-in-rats-through-pi3k-akt-signaling-pathway-by-targeting-insulin-like-growth-factor-2
#19
Xihua Lu, Shuaiguo Lv, Yan Mi, Lei Wang, Gensheng Wang
The aim of this study was to investigate the in vivo and in vitro effects of miR-665 on sevoflurane anesthesia-induced cognitive dysfunction. SH-SY5Y cells and male SD rats were treated with sevoflurane to simulate anesthesia-induced cognitive dysfunction. The cells and rats both were transfected with a miR-665 mimic, inhibitor, scramble, IGF-2 siRNA, or treated with P13K/Akt inhibitor LY294002. The cell apoptosis, autophagy, growth related proteins, and mRNA levels were measured using different methods. The motor performance was assessed using the Morris water maze (MWM) test...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386359/increased-th9-cells-and-il-9-levels-accelerate-disease-progression-in-experimental-atherosclerosis
#20
Qing Li, Tingting Ming, Yuanmin Wang, Shaowei Ding, Chaojie Hu, Cuiping Zhang, Qi Cao, Yiping Wang
Atherosclerosis (AS) is the number one killer in developed countries, and currently considered a chronic inflammatory disease. The central role of T cells in the pathogenesis of atherosclerosis is well documented. However, little is known about the newly described T cell subset-Th9 cells and their role in AS pathogenesis. Here, the amounts of Th9 cells as well as their key transcription factors and relevant cytokines during atherosclerosis were assessed in ApoE(-/-) mice and age-matched C57BL/6J mice. Significantly increased Th9 cell number, Th9 related cytokine (IL-9), and key transcription factor (PU...
2017: American Journal of Translational Research
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