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American Journal of Translational Research

Weixing Wang, Hong Tan, Hua Liu, Huabao Peng, Xiaoyan Li, Xiqiang Dang, Xiaojie He
Preglomerular arteriopathy (PA) induced by hyperuricemia contributes to the progression of chronic kidney disease (CKD). Green tea polyphenols (GTPs) are antioxidant ingredients thought to assist in preventing hyperuricemia. However, the underlying mechanism by which GTPs affect renal function remains unclear. Both normal and remnant kidney (RK) rats were administrated oxonic acid (OX) to induce hyperuricemia. The hyperuricemia RK rats were concomitantly treated with GTPs. Hematoxlyin-eosin (H&E) and periodic acid-Schiff (PAS) staining methods were used to examine renal function and arterial morphology...
2018: American Journal of Translational Research
Martina Ramella, Priscilla Bernardi, Luca Fusaro, Marcello Manfredi, Francesco Casella, Carla M Porta, Laura Nicolai, Edoardo Galeazzi, Renzo Boldorini, Alberto M Settembrini, Piergiorgio Settembrini, Emilio Marengo, Mario Cannas, Francesca Boccafoschi
Aneurysm is a multifactorial degenerative disease characterized by focal dilatation of blood vessels. Although abdominal aortic (AAA) and popliteal aneurysms (PAA) are the most common dilatative vascular diseases and share some features, a comparison between the different anatomical sites and the relative pathophysiological differences has not been established. In order to gain deeper insights to AAA and PAA, we have characterized the role of matrix remodelling, vascular cells phenotype depletion and the inflammatory process in both diseases...
2018: American Journal of Translational Research
Richard S Garner, Rakib U Rayhan, James N Baraniuk
One third of Gulf War Illness (GWI) subjects in a recent study were found to develop transient postural tachycardia after submaximal exercise stress tests. Post-exercise postural tachycardia is a previously undescribed physiological finding. A new GWI cohort was studied to verify this novel finding and characterize this cardiovascular phenomenon. Subjects followed the same protocol as before. The change in heart rate between recumbent and standing postures (ΔHR) was measured before exercise, and after submaximal bicycle exercise...
2018: American Journal of Translational Research
Yoichi Ohtaki, Kyoichi Kaira, Jun Atsumi, Toshiteru Nagashima, Osamu Kawashima, Takashi Ibe, Mitsuhiro Kamiyoshihara, Ryoichi Onozato, Atsushi Fujita, Tomohiro Yazawa, Masayuki Sugano, Misaki Iijima, Seshiru Nakazawa, Kai Obayashi, Takayuki Kosaka, Toshiki Yajima, Hiroyuki Kuwano, Ken Shirabe, Akira Mogi, Kimihiro Shimizu
OBJECTIVES: Since large cell neuroendocrine carcinoma (LCNEC) is a relatively rare histologic type of primary lung cancer, little is known about the immunological status of patients with LCNEC. We aimed to clarify the expression and prognostic impact of programmed cell death ligand 1 (PD-L1), CD8, CD4, and Forkhead box protein P3 (Foxp3) in LCNEC. METHODS: We retrospectively analyzed PD-L1, CD8, CD4, and Foxp3 expressions in 95 surgically resected LCNEC. PD-L1 positive staining was determined in tumors with more than 1% of tumor cells stained to any intensity, and CD8, CD4, and Foxp3 positivity was determined in tumors with more than 5% of lymphocytes stained...
2018: American Journal of Translational Research
Yuxing Jiang, Yongliang Zhao, Feng Qian, Yan Shi, Yingxue Hao, Jun Chen, Pingang Li, Peiwu Yu
BACKGROUNDS AND PURPOSE: Robotic surgery has been applied in gastric carcinoma over a decade. Although a series of studies were performed to investigate the short-term outcomes of robot-assisted gastrectomy, few papers were in view of long-term outcomes. The current study was aimed to explore the oncological outcomes of robotic gastrectomy for gastric cancer patients. METHODS: A total of 606 gastric cancer patients who underwent robot-assisted gastrectomy during March 2010 through March 2017, were enrolled in this research...
2018: American Journal of Translational Research
Zixuan Sun, Jingyan Chen, Jiao Zhang, Runbi Ji, Wenrong Xu, Xu Zhang, Hui Qian
MicroRNAs (miRNAs) play important roles in cell transformation and carcinogenesis. We have previously established a tumor cell line K3 transformed from rat bone marrow-derived mesenchymal stem cells (rBM-MSCs). However, the underlying mechanism involved in MSC transformation remains unclear. Herein, we identified the key miRNAs that regulate the transformation of rBM-MSCs, and clarified their biological roles. Microarray and qRT-PCR results showed an increased expression of miR-374 but decreased expressions of miR-199a, miR-145, miR-34a, and miR-214 in K3 cells compared to rBM-MSCs...
2018: American Journal of Translational Research
Zhaocai He, Xin Wang, Changhao Huang, Yu Gao, Chen Yang, Pengwei Zeng, Zihua Chen
To explore the effect of fetal-lethal non-coding developmental regulatory RNA (FENDRR) in the initiation and progression of gastric cancer (GC). We detected the levels of FENDRR, microRNA-214-3p (miR-214-3p), and ten-eleven-translocation (TET2) in GC tissues and GC cell lines. In addition, we evaluated the location of FENDRR in GC cell lines by fluorescence in situ hybridization (FISH). Cell proliferation and apoptosis were measured by CCK-8 and Hoechst staining assays. Methylation-specific PCR assay (MSP) was used to evaluate the methylation status of ras-association domain family 1A (RASSF1A)...
2018: American Journal of Translational Research
Zhiling Qiu, Yuanhui Hu, Yanting Geng, Huaqin Wu, Rongqiang Bo, Jingjing Shi, Jiuchong Wang, Huan Wang
Heart failure caused by myocardial infarction is a common cardiovascular disease with high mortality rate. Myocardial mitophagy is involved in the process of occurrence and development of heart failure. In this study, we aimed to investigate the effects of Xin Fu Kang (XFK) oral liquid on myocardial mitophagy in a rat model of advanced heart failure. The rat model of advanced heart failure was established by ligating the left anterior descending (LAD) artery for eight weeks. Captopril and XFK were given by gavage separately...
2018: American Journal of Translational Research
Zhihua Li, Liang Xu, Yong Liu, Shaokun Fu, Jianhong Tu, Yangyang Hu, Qiuyun Xiong
Postoperative fever is prevalent in many breast cancer patients. Some retrospective studies proposed that postoperative fever might also be considered as a rapid rough indicator for the poor prognosis of breast cancer patients. This study aims to explore the possible molecular mechanisms underlying the relapse of breast cancer patients with early postoperative fever. Our results indicated plasma levels of lncRNA MALAT1 were elevated in breast cancer patients with early postoperative fever and were associated with RFS...
2018: American Journal of Translational Research
Wei-Quan Zhang, Shu-Kang Zhao, Jun-Wen Luo, Xiao-Peng Dong, Ying-Tao Hao, Hui Li, Lei Shan, Yong Zhou, Hu-Bo Shi, Zai-Yun Zhang, Chuan-Liang Peng, Xiao-Gang Zhao
Emerging evidence suggests the microbiome may affect a number of diseases, including lung cancer. However, the direct relationship between gut bacteria and lung cancer remains uncharacterized. In this study, we directly sequenced the hypervariable V1-V2 regions of the 16S rRNA gene in fecal samples from patients with lung cancer and healthy volunteers. Unweighted principal coordinate analysis (PCoA) revealed a clear difference in the bacterial community membership between the lung cancer group and the healthy control group...
2018: American Journal of Translational Research
Ricardo Lamy, Elliot Chan, On-Tat Lee, Audrey Phone, Vasant A Salgaonkar, Chris J Diederich, Jay M Stewart
Clinical management of many chronic ophthalmological disorders requires direct delivery of drugs into the vitreous. There is an important need to investigate novel needle-less alternatives to deliver drugs to the vitreous. The purpose of this study is to assess the effects of a needle-less system using ultrasound to enhance vitreal delivery of small molecules through the sclera in an ex vivo model and to evaluate whether changes in permeability are mainly due to the heat generated by sonication. An eye cup containing 1 mL of sodium fluorescein 0...
2018: American Journal of Translational Research
Mengjiao Zhu, Hanfei Tang, Xiao Tang, Xiaoyun Ma, Daqiao Guo, Fengshan Chen
Circadian rhythm disruption is intimately linked to atherosclerosis, and endothelial-to-mesenchymal transition (EndMT) is a major feature of atherosclerosis progression and unstable plaques. However, the mechanisms underlying the roles of Brain and Muscle ARNT-Like Protein-1 (BMAL1), an essential clock transcription activator, in EndMT and plaque instability have not been characterized. In the present study, we found a positive relationship among BMAL1 expression loss, EndMT, and plaque vulnerability in human carotid plaques...
2018: American Journal of Translational Research
Yi-Wen Lin, Chun-Yao Huang, Chun-Min Shih, Yi-Ting Tsai, Chin-Sheng Lin, Chih-Yuan Lin, Chi-Yuan Li, Shih-Hurng Loh, Cheng-Yen Lin, Feng-Yen Lin, Chien-Sung Tsai
Cardiopulmonary bypass (CPB) induces cytokine production and causes postoperative monocytic inflammatory responses, which are associated with patient outcomes. In fact, monocytes regulate immunity through dynamic networks of survival and cellular apoptosis as well as thrombomodulin (TM)-associated differenciiation. Whether CPB affects the plasma level of eotaxin-2, a potent chemoattractant, or stimulates monocyte apoptosis among patients undergoing elective coronary artery bypass graft (CABG) surgery is also unknown...
2018: American Journal of Translational Research
Xiaoliang Zhu, Xuefeng Rao, Wu Yao, Xia Zou
Breast cancer is considered to be the most frequently diagnosed malignancy in women worldwide. MicroRNAs (miRNAs) play key roles in the regulation of tumor properties based on their capacity to regulate the expression of tumor-related genes. However, the involvement of miR-196b-5p in breast cancer development is largely unknown. Here, we showed that the expression levels of miR-196b-5p were significantly down-regulated in breast cancer samples and cell lines compared to the matched normal tissues and breast epithelial cell line, respectively...
2018: American Journal of Translational Research
Ailing Yu, Leilei Mao, Fangfang Zhao, Baoliang Sun
Chronic cerebral hypoperfusion (CCH) has become a crucial factor contributing to neurological disorders and cognitive deficits. Olfactory ensheathing cells (OECs) transplantation has been widely used to repair central nerve systems (CNS) injury, however, whether this intervention has therapeutic effects on CCH-induced cognitive dysfunction and brain damages is still unknown. In this study, we sought to determine the potential therapeutic effects of OECs transplantation on CCH. Two days after the establishment of 2VO rat model, OECs or its medium transplantation were performed via intrastriatal injection...
2018: American Journal of Translational Research
An Hu, Hai-Bin Liu, Robert Mlynski, Stefan Plontke, Jing-Fei Zhang, Wei-Jun Dai, Jun-Li Duan, Jing-Ping Fan, Hong-Liang Zheng, Wei-Hua Xu, Xiao-Ping Chen, Jing-Juan Huang
Background: Postoperative pain has well defined and is perceived by patients as one of the most obnoxious aspects of surgical pain. The aim of this study was to determine whether the combination of Therapeutic ultrasound (TUS) and Curcumin (CUR) resulted in an enhancement of their pain relieving activities in a rat model of postoperative pain. Methods: We explored the effect of these treatment and their interaction with signal transduction pathways involved in inflammatory. In this study, TUS and CUR alone or in combination were administered prior to or simultaneously with or after the incisional surgery...
2018: American Journal of Translational Research
Ya Zheng, Jie Zhu, Haiyan Zhang, Yanmei Liu, Hong Sun
The purpose of the current study was to investigate whether metformin can enhance the anti-cancer effect of cisplatin on epithelial ovarian cancer in vitro and in vivo. CCK-8 assays were performed to detect cell viability, and flow cytometry was performed to measure cell apoptosis rates. Transwell assays were used to detect the migration and invasion ability of ovarian cancer cells. Western blotting and qRT-PCR were performed to detect protein expression. Xenograft mouse models were constructed to clarify the treatment response in vivo...
2018: American Journal of Translational Research
Xuezong Wang, Yan Xue, Weiwei Ye, Jian Pang, Zhenfen Liu, Yuelong Cao, Yuxin Zheng, Daofang Ding
The aim of this study was to investigate the role of the mitogen-activated protein kinase kinase-extracellular signal-regulated kinases 1/2 (MEK-ERK1/2) signaling pathway in chondrocyte differentiation and cartilage tissue construction in vitro . Chondrocytes were stimulated with rat serum (RS) and fetal bovine serum (FBS), and chondrocyte phenotypes were investigated microscopically. Chondrocyte proliferation was analyzed using fluorescence activated cell sorting (FACS) and the CCK8 method. Protein and mRNA expressions were assessed by western blot and RT-qPCR...
2018: American Journal of Translational Research
Sheung-Fat Ko, Yen-Ta Chen, Christopher Glenn Wallace, Kuan-Hung Chen, Pei-Hsun Sung, Ben-Chung Cheng, Tien-Hung Huang, Yi-Ling Chen, Yi-Chen Li, Hsueh-Wen Chang, Mel S Lee, Chih-Chao Yang, Hon-Kan Yip
This study tested whether inducible pluripotent stem cell (iPSC)-derived mesenchymal stem cell (MSC) therapy could effectively protect kidney from acute ischemia (1 h) - reperfusion (5 day) injury (IRI). Male-adult SD-rats (n = 24) were equally categorized into groups 1 (sham-control), 2 [sham-control + iPSC-MSC (1.2 × 106 cells/rat)], 3 (IR only) and 4 (IR + iPSC-MSC). Blood urine nitrogen/creatinine levels and ratio of urine protein to creatinine, kidney weight and expressions of inflammation (TNF-α/NF-κB), oxidative-stress (NOX-1/NOX-2/oxidized protein) and apoptosis (mitochondrial-Bax/cleaved caspase-3/PARP) were significantly higher in group 3 than in groups 1, 2 and 4 and significantly higher in group 4 than in groups 1 and 2 (all P<0...
2018: American Journal of Translational Research
Wen-Yan Wang, Yun-Xia Cao, Xiao Zhou, Bing Wei, Lei Zhan, Liu-Tao Fu
Many cervical cancer (CC) patients suffer from cancer invasion and lymph node metastasis, resulting in poor therapeutic outcome. Evidence has indicated the involvement of misexpressed high-mobility group AT-hook 2 (HMGA2) in poor survival of cancer patients. This study hereby aims to investigate the role of HMGA2 in CC cell biological functions via the ATR/Chk1 signaling pathway. The cell line with the highest HMGA2 expression was selected to establish cell lines with wild-type and stable HMGA2 silencing. The underlying regulatory mechanisms of HMGA2 in CC cells were analyzed with the treatment of the ATR/Chk1 signaling pathway activator, inhibitor, shRNA against HMGA2 or pcDNA-HMGA2 plasmids, followed by quantification of expression levels of ATR, Chk1, Bcl-2, Bax, MMP-2, MMP-9, E-cadherin and N-cadherin...
2018: American Journal of Translational Research
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