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American Journal of Translational Research

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https://www.readbyqxmd.com/read/28337310/upregulation-of-tspan12-is-associated-with-the-colorectal-cancer-growth-and-metastasis
#1
Jiao Liu, Chuang Chen, Guang Li, Dechang Chen, Qingshan Zhou
Tetraspanin 12 (TSPAN12), as an earliest member of tetraspanin family, has been recently shown to be highly expressed in several malignant tumors, such as lung cancer and breast cancer, which plays an important role in regulating cell proliferation, migration and invasion. However, the functional roles of TSPAN12 in colorectal cancer (CRC) remain largely unclear. In this study, the expression of TSPAN12 was up-regulated compared to that in paracarcinoma tissues. Higher TSPAN12 expression was significantly correlated with TNM stage, tumor size and lymph node metastasis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337309/gkn2-increases-apoptosis-reduces-the-proliferation-and-invasion-ability-of-gastric-cancer-cells-through-down-regulating-the-jak-stat-signaling-pathway
#2
Jun Ouyang, Xiaohui Pan, Hui Lin, Zecheng Hu, Ping Xiao, Haobin Hu
OBJECTIVES: To investigate the effect of gastric motility protein 2 (GKN2) on the proliferation, apoptosis and invasion of gastric cancer cell and on the JAK/signal transducer and activator of transcription 3 (STAT3) signaling pathway. METHODS: Expression of GKN2 was qualified using Western blot analysis in four gastric cancer cell lines and immortalized human gastric mucosal epithelial cell line GES-1. The cells were then transfected with pcDNA3.1-GKN2 and control vector using Lipofectamine(tm)2000 and assayed for viability, apoptosis, cell cycle changes, invasion ability as well as expression of cell cycle protein D1 (Cylin D1), Bcl-2, Bax, matrix metalloproteinase 2 (MMP2), MMP9, JAK2 and p-STAT3...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337308/erratum-effect-of-granatin-b-on-the-glioma-cancer-by-inducing-apoptosis
#3
Zheng Jin, Ying Yu, Ri-Hua Jin, Yu-Bo Wang, Hai-Yang Xu
[This corrects the article on p. 3970 in vol. 8, PMID: 27725877.].
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337307/effects-of-microrna-29a-on-retinopathy-of-prematurity-by-targeting-agt-in-a-mouse-model
#4
Xin-Ke Chen, Li-Juan Ouyang, Zheng-Qin Yin, Yuan-You Xia, Xiu-Rong Chen, Hui Shi, Yan Xiong, Lian-Hong Pi
BACKGROUND: This study aimed to explore the effects of microRNA-29a (miR-29a) on retinopathy of prematurity (ROP) by targeting angiotensinogen (AGT) expression in a mouse model. METHODS: Ninety-six C57BL/6J mice were selected and divided into the normal control group (n = 12) and the oxygen-induced retinopathy (OIR) group (n = 84). All the mice in the OIR group were assigned to the following seven groups (12 mice in each group): the blank, miR-29a mimics, miR-29a inhibitors, empty plasmid, miR-29a mimics + si-AGT, miR-29a inhibitors + si-AGT and si-AGT groups...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337306/thymoquinone-potentiates-chemoprotective-effect-of-vitamin-d3-against-colon-cancer-a-pre-clinical-finding
#5
Amr M Mohamed, Bassem A Refaat, Adel G El-Shemi, Osama A Kensara, Jawwad Ahmad, Shakir Idris
Prevention of colon cancer among high-risk group has been long lasting research goal. Emerging data have evidenced the anticancer activities of Vitamin D3 (Vit.D) and Thymoquinone (TQ). The aim of the current study was to evaluate the synergistic potential of Thymoquinone and Vitamin D3 in the control of colon cancer progression using azoxymethane-induced rat model. Vit.D and TQ were given individually or in combination 4 week prior to induction and continued for a total of 20 week. At the end of the study, all animals were euthanized and their resected colons were examined macroscopically and microscopically for tumor growth...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337305/rho-kinase-regulates-neurite-outgrowth-of-hippocampal-neurons-via-calcium-dependent-cytoskeleton-regulation
#6
Zhisheng Ji, Zhenbin Cai, Jifeng Zhang, Nannuan Liu, Jing Chen, Minghui Tan, Hongsheng Lin, Guoqing Guo
OBJECTIVE: To investigate whether calcium is involved in downstream signal transduction in neurite outgrowth regulated by Rho kinase. METHODS: In vitro primary hippocampal neurons were cultured and treated with Rho kinase agonist (LPA) or antagonist (Y-27632). Then, the cytoskeleton and neurite outgrowth were observed. After addition of calcium antagonist BAPTA/AM to reduce intracellular calcium, the cytoskeleton distribution and neurite outgrowth were observed...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337304/down-regulation-of-mediator-complex-subunit-19-med19-induces-apoptosis-in-human-laryngocarcinoma-hep2-cells-in-an-apaf-1-dependent-pathway
#7
Yan Zhao, Qingfeng Meng, Xu Gao, Lihua Zhang, Lixin An
Mediator 19 (Med19) is a component of the mediator complex which is a co-activator for DNA-binding factors that activate transcription via RNA polymerase II. Accumulating evidence has shown that Med19 plays important roles in cancer cell proliferation and tumorigenesis. The physiological mechanism by which Med19 exerts its promoting effects in laryngocarcinoma is not yet fully understood. Here, we found that the expression of Med19 was increased in laryngocarcinoma samples from patients compared to normal bone tissues...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337303/the-involvement-and-possible-mechanism-of-nr4a1-in-chondrocyte-apoptosis-during-osteoarthritis
#8
Xinge Shi, Hui Ye, Xuedong Yao, Yanzheng Gao
Osteoarthritis (OA) is a joint disease caused by the breakdown of joint cartilage and underlying bone, and places great burdens to daily life of patients. Nuclear orphan receptor nuclear receptor subfamily 4, group A, member 1 (NR4A1) is vital for cell apoptosis, but little is known about its role in OA. This study aims to reveal the expression and function of NR4A1 during OA chondrocyte apoptosis. NR4A1 expression by qRT-PCR and western blot, and chondrocyte apoptosis by TUNEL assay were detected in normal and OA joint cartilage...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337302/different-impairment-of-immune-and-inflammation-functions-in-short-and-long-term-after-ischemic-stroke
#9
Wen-Xing Li, Fei Qi, Jia-Qian Liu, Gong-Hua Li, Shao-Xing Dai, Tao Zhang, Fei Cheng, Dahai Liu, Song Guo Zheng
Ischemic stroke therapy and prognosis outcomes largely depend on the time periods after symptom onset. This study aims to explore the difference of global gene expression profiles and impairment of biological functions between short-term and long-term after stroke onset. We compared three short-term (3 h, 5 h and 24 h) and a long-term (6-month) gene expression levels by a multi-platform microarray data integration method. RankProd was used to calculate the differentially expressed genes between stroke patients and controls...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337301/endoplasmic-reticulum-stress-a-new-wrestler-in-the-pathogenesis-of-idiopathic-pulmonary-fibrosis
#10
Lei Zhang, Yi Wang, Nuruliarizki Shinta Pandupuspitasari, Guorao Wu, Xudong Xiang, Quan Gong, Weining Xiong, Cong-Yi Wang, Ping Yang, Boxu Ren
Idiopathic pulmonary fibrosis (IPF) has attracted extensive attention for its unexplained progressive lung scarring, short median survival and its unresponsiveness to traditional therapies. Despite extensive studies, the mechanisms underlying IPF pathoetiologies, however, remain poorly understood. Recent advances delineated a potential function of endoplasmic reticulum (ER) stress in meeting the need of fibrotic response, which pinpointed a critical role for the unfolded protein response (UPR) pathways in IPF pathogenesis...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337300/mutation-of-mir-21-targets-endogenous-lipoprotein-receptor-related-protein-6-and-nonalcoholic-fatty-liver-disease
#11
Chang-Ping Li, Hong-Jue Li, Jiao Nie, Xia Chen, Xian Zhou
Nonalcoholic fatty liver disease (NAFLD) is a chronic disorder characterized by hepatic fat accumulation and abnormal lipid metabolism. Although miR-21 has been implicated in nonalcoholic fatty liver disease, it is unknown whether miR-21 could function as a therapeutic target. Here, we perform transfection analysis of miR-21 mimic or control mimic to evaluate the effects of miR-21 expression levels on human HepG2 nonalcoholic fatty liver cells. We used siRNA techniques to knock down miR-21 in HepG2 and control 293T cell lines, and then monitored lipid production and the expression levels of genes involved in lipid metabolism...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337299/poly-r-c-binding-protein-pcbp-1-expression-is-regulated-at-the-post-translation-level-in-thyroid-carcinoma
#12
Ming-Peng Zhang, Wei-San Zhang, Jin Tan, Ming-Hui Zhao, Lin-Juan Lian, Jie Cai
Poly r(C) binding protein (PCBP) 1 or heterogeneous ribonucleoprotein (hnRNP) E1 is a RNA binding protein that plays a vital role in a wide variety of biological processes. PCBP1 has been shown to function as a tumor suppressor by negatively regulating translation of pro-metastatic proteins in different cancers. Loss of PCBP1 expression or its Akt2-mediated phosphorylation at serine 43 residue has both been indicated to de-repress its regulation of EMT inducer proteins. Our previous work has established that PCBP1 functions as a tumor suppressor in thyroid cancer, where its translation is inhibited by microRNA-490-3p...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337298/xbp1-lox-axis-is-critical-in-er-stress-induced-growth-of-lung-adenocarcinoma-in-3d-culture
#13
Yi Yang, Bai-Jun Cheng, Hong Jian, Zhi-Wei Chen, Yi Zhao, Yong-Feng Yu, Zi-Ming Li, Mei-Lin Liao, Shun Lu
Rapid growth of tumor cells needs to consume large amounts of oxygen and glucose, due to lack of blood supply within the tumor, cells live in an environment that lack of oxygen and nutrients. This environment results in endoplasmic reticulum (ER) stress and activates the UPR (unfolded protein response). More and more evidence suggests UPR provides a growth signal pathway required for tumor growth. In the present study, we investigated the relationship between XBP1, one transcription factor in UPR, and the expression of LOX...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337297/protective-effect-of-a-novel-antibody-against-tlr2-on-zymosan-induced-acute-peritonitis-in-nf-%C3%AE%C2%BAb-transgenic-mice
#14
Qingjun Pan, Jun Cai, Yanxia Peng, Haiyan Xiao, Lifang Zhang, Jinying Chen, Huafeng Liu
In addition to antibiotic therapy for treatment of peritonitis, biologics have also been found to exhibit both anti-inflammatory and inflammation-resolving properties. Here, we first developed NF-κB transgenic mice with zymosan-induced acute peritonitis to investigate the effects of a novel anti-Toll-like receptor (TLR)2 antibody (anti-T20). In this mouse model, anti-T20 treatment significantly attenuated the increase of peritoneal NF-κB activity and serum levels of inflammatory cytokines, including monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in a dose-dependent manner compared to mice treated with isotype control antibody...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337296/tumor-suppressor-p53-induces-mir-15a-processing-to-inhibit-neuronal-apoptosis-inhibitory-protein-naip-in-the-apoptotic-response-dna-damage-in-breast-cancer-cell
#15
Li Yang, Wei Zhao, Ping Wei, Wenshu Zuo, Shouhui Zhu
This study was aimed to investigate the functional role of miR-15a in breast cancer cells in response to DNA damage and to illustrate the possible potential underlying molecular mechanism(s). Human breast cancer cell lines MCF-7 cells and/or MDA-MB-231 cells were pre-treated with or without bleomycin. Cells were transfected with corresponding vectors. qRT-PCR was used to detect the expression of mRNA or miRNA, and immunoprecipitation and immunoblot analysis were performed to explore the status of protein association...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337295/rna-helicase-dhx9-may-be-a-therapeutic-target-in-lung-cancer-and-inhibited-by-enoxacin
#16
Shiguang Cao, Ruiying Sun, Wei Wang, Xia Meng, Yuping Zhang, Na Zhang, Shuanying Yang
RNA helicase DHX9 is a member of human RNA enzymes. Previous studies have reported that DHX9 is highly expressed in various types of malignant tumor. However, its role in the progression of lung cancer remains to be fully clarified. The present study aims to investigate the oncogenic role of DHX9 in serum, tissues and lung cancer cell lines in vitro. We used RNA interference to downregulate DHX9 expression in A549 cells using a small interfering RNA lentiviral vector. Subsequently, enoxacin was used to inhibit cell proliferation, and this effect was detected using MTT...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337294/hypoxic-preconditioning-of-human-cardiosphere-derived-cell-sheets-enhances-cellular-functions-via-activation-of-the-pi3k-akt-mtor-hif-1%C3%AE-pathway
#17
Yuya Tanaka, Tohru Hosoyama, Akihito Mikamo, Hiroshi Kurazumi, Arata Nishimoto, Koji Ueno, Bungo Shirasawa, Kimikazu Hamano
Cell sheet technology is a promising therapeutic strategy for the treatment of ischemic diseases such as myocardial infarction. We recently developed a novel protocol, termed "hypoxic preconditioning," capable of augmenting the therapeutic efficacy of cell sheets. Following this protocol, the pro-angiogenic and anti-fibrotic activity of cell sheets were enhanced by brief incubation of cell sheets under hypoxic culture conditions. However, the precise molecular mechanism underlying the hypoxic preconditioning of cell sheets is unclear...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337293/mir-146b-protects-cardiomyocytes-injury-in-myocardial-ischemia-reperfusion-by-targeting-smad4
#18
Yun-Feng Di, De-Cai Li, Yan-Qing Shen, Chun-Lei Wang, Da-Yong Zhang, An-Quan Shang, Teng Hu
MicroRNAs, a class of small and non-encoding RNAs that transcriptionally or post-transcriptionally modulate the expression of their target genes, have been implicated as critical regulatory molecules in many cardiovascular diseases, including ischemia-/reperfusion-induced cardiac injury. In the present study, we report on the role of miR-146b in myocardial I/R injury and the underlying cardio-protective mechanism. Antagomir-146b was used to explore the effects of miR-146b on cardiac ischemia/reperfusion injury (30 min ischemia followed by 180 min reperfusion)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337292/mir-217-promotes-cutaneous-squamous-cell-carcinoma-progression-by-targeting-ptrf
#19
Ming Bai, Mingzi Zhang, Fei Long, Nanze Yu, Ang Zeng, Xiaojun Wang
Increasing evidences have suggested that microRNAs (miRNAs) act a critical role in tumor initiation, progression and metastasis. Deregulated expression of miR-217 has been identified in various tumors. However, the expression and role of miR-217 in the development of cutaneous squamous cell carcinoma (cSCC) remain unclear. In our study, we showed that miR-217 expression was upregulated in the cSCC tissues compared to adjacent non-tumor samples. We also demonstrated that miR-217 expression was upregualted in the cSCCcSCC cell lines...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337291/cholecystokinin-attenuates-radiation-induced-lung-cancer-cell-apoptosis-by-modulating-p53-gene-transcription
#20
Yi Han, Chongyu Su, Daping Yu, Shijie Zhou, Xiaoyun Song, Shuku Liu, Ming Qin, Yunsong Li, Ning Xiao, Xiaoqing Cao, Kang Shi, Xu Cheng, Zhidong Liu
The deregulation of p53 in cancer cells is one of the important factors by which cancer cells escape from the immune surveillance. Cholecystokinin (CCK) has strong bioactivity in the regulation of a number of cell activities. This study tests a hypothesis that CCK interferes with p53 expression to affect the apoptotic process in lung cancer (tumor) cells. In this study, tumor-bearing mice and A549 cells (a tumor cell line) were irradiated. The expression of CCK and p53 in tumor cells was assessed with RT-qPCR and Western blotting...
2017: American Journal of Translational Research
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