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American Journal of Translational Research

Haiyu Wang, Lu Yao, Yuda Gong, Bo Zhang
Tripartite motif 31(TRIM31) is a new member of the E3 ubiquitin ligase family, which plays a role in many biological processes. It has been indicated that TRIM31 is strongly correlated with tumorigenesis. However, the impact of TRIM31 in colorectal cancer (CRC) and its underlying mechanisms are largely unknown. In this study, we found an increase in TRIM31 expression in CRC cells and a positive correlation between TRIM31 and CRC malignancy. Applying genetic interventions and cellular function tests, our results showed that TRIM31 acted as an epithelial-mesenchymal transition (EMT) inducer to promote CRC invasion and metastasis...
2018: American Journal of Translational Research
Yaqin Lu, Qi Liu, Qin Yu
The aim of this study was to evaluate the protective effects of dietary flavonoid quercetin against Alzheimer's disease (AD) and detect the explicit administration of quercetin in early-middle or middle-late stage of AD pathology could play the effect as well as its mechanism of action. In this study, APP/PS1 mice were used to investigate cognitive impairment and related pathologies. The results showed that quercetin enrich diet could play an ameliorated pathology development of AD in APP/PS1 mice. And then we next determined administration of quercetin in early-middle and middle-late stage of AD pathology, which exerted that only quercetin enrich diet during the early-middle stage of AD pathological development period ameliorates cognitive dysfunction and the protection effect was mainly related to increased Aβ clearance and reduced astrogliosis...
2018: American Journal of Translational Research
Wenxia Xu, Qiqi Shi, Xiaoling Qian, Bingluo Zhou, Jinye Xu, Liyuan Zhu, Lifeng Feng, Hongchuan Jin, Xian Wang
Cancers are huge problems that need to be investigated thoroughly. Rab5a plays an important part in the regulation of intracellular membrane trafficking. However, its role in cancer and autophagy has not been fully determined. In this study, we analyzed the correlation between Rab5a expression and patients' prognosis and then explored the effect of Rab5a knockdown on different cell lines using western blotting and fluorescence. Our results showed that up-regulated Rab5a positively correlated with the prognosis of gastric cancer patients...
2018: American Journal of Translational Research
Shaohua Tang, Ming Xiaoming Zhang, Shengbo Yang
This study aimed to identify the location and depth of the center of intramuscular nerves dense regions (CINDRs) in anterior brachial muscles. Twelve adult cadavers were used. One side anterior brachial muscles were isolated and subjected to Sihler's staining to show intramuscular nerve dense regions. Then the data was used to localize CINDRs in situ on the same muscles of the contralateral side. The localization method involved dissection and exposure of CINDRs, barium sulfate labeling, body surface reference line design, spiral computed tomography scan, three-dimensional image reconstruction, and Syngo system measurements...
2018: American Journal of Translational Research
Qian Tang, Minji Tong, Gang Zheng, Liyan Shen, Ping Shang, Haixiao Liu
The Masquelet's induced membrane (IM) technique is widely used to treat large segmental bone defects due to its physical priority and biological function. However, the underlying molecular mechanism of the IM on bone formation remains unknown. In the present study, rat bone marrow-derived mesenchymal stem cells (BMSCs) were used as an in vitro model and bone morphogenetic protein 2 (BMP-2) was used as a positive control to evaluate the effects of the IM on the osteogenic differentiation of BMSCs. Although the IM group did not exhibit a significant increase in the expression of Runt-related transcription factor 2 (Runx2), Collagen I (Col I), osteocalcin (OCN) and alkaline phosphatase (ALP) relative to the BMP-2 administration, the IM was considerably effective compared with the untreated group...
2018: American Journal of Translational Research
Yanling Zhao, Wenwen Huang, Jiali Wang, Yan Chen, Wen Huang, Yonglin Zhu
The current study evaluates the effects of taxifolin (TA) on streptozotocin (STZ)-induced diabetic nephropathy in rats. We performed oral glucose tolerance tests (OGTTs) to determine blood glucose levels. We also measured the following biochemical parameters: uric acid, creatinine, and serum insulin. Kidney pathology was examined by staining sections with hematoxylin-eosin (H&E). The expression of Caveolin-1 and NF-κB was analyzed by qRT-PCR and western blotting. TA significantly reduced the concentrations of blood glucose, uric acid, creatinine, and serum insulin in STZ-induced diabetic rats...
2018: American Journal of Translational Research
Xiang Tang, Bin Li, Jing Ding, Lei Zhang, Lan Zhu
OBJECTIVE: To analyze gene expression profiles analysis during the differentiation of sheep muscle-derived stem cells (MDSCs) into smooth muscle cells (SMCs) in sheep. METHODS: A modified preplate technique was employed to isolation of the MDSCs from sheep. The MDSCs were subjected to flow cytometry analysis targeting CD44, CD31, CD45, CD14, and CD49f and were treated with TGF-β1 at a concentration of 10 ng/ml for ten days. The expression levels of smooth muscle α-actin (α-SMA) and calponin after treatment with TGF-β1 were determined by western blotting and immunofluorescence staining...
2018: American Journal of Translational Research
Qingxiong Yu, Min Wu, Lingling Sheng, Qingfeng Li, Feng Xie
Most giant congenital melanocytic nevi (GCMN) exhibit an activating mutation in NRAS. Constitutive activation of the RAS-ERK signaling pathway induces proliferation in nevus cells and plays a pivotal role in melanoma development. In this study, we studied the efficacy of RAS-ERK pathway targeted therapy in GCMN. We isolated nevus cells from GCMN (GNCs) and compared the morphology of GNCs with normal melanocytes and the A375 melanoma cell line. Proliferation curves of GNCs and A375 cells were determined using Cell Counting Kit-8 assays...
2018: American Journal of Translational Research
Le Han, Wenjuan Chen, Yanmin Xia, Yangrong Song, Zheng Zhao, Hao Cheng, Tao Jiang
MicroRNAs (miRNAs) are involved in the development and progression of lung cancer. MicroRNA-101 (miR-101) displays crucial properties in non-small cell lung cancer (NSCLC) by negatively regulating cell proliferation and invasion, but the underlying molecular mechanisms remain largely unknown. In this study, we found that miR-101 was underexpressed while zinc finger E-box binding homeobox 1 (ZEB1) was highly upregulated in NSCLC tissues and cells. The downregulation of miR-101 was positively associated with lymph node metastasis and poor prognosis of NSCLC patients...
2018: American Journal of Translational Research
Xiaofeng Chen, Jiangyuan Du, Rui Jiang, Ling Li
Increasing evidence suggests that microRNAs (miRNAs) play a crucial role in the pathogenesis of tumor. In this study, miR-214 was found to be significantly down-regulated in lung tumor tissues and lung cell lines. From the gain-of-function experiment results, we found that ectopic expression of miR-214 in lung cancer cell lines significantly inhibited cell growth, as evidenced by cell viability and colony formation assays, and suppressed tumor growth in vivo . Besides, further investigations showed that miR-214 inhibited cell migration and invasion...
2018: American Journal of Translational Research
Jianjun Xu, Caijiao Lu, Zhengjun Liu, Peng Zhang, Hailei Guo, Tingting Wang
Schizandrin B ( Sch B ) is the main component isolated from Schizandra fruit ( Schisandra chinensis ). While Sch B is established as having antioxidant, anti-proliferation and anti-inflammatory properties, but its activity in sepsis remains unclear. In the present study, we investigated the anti-inflammatory effects of Sch B in sepsis. Our experimental results demonstrated that Sch B inhibited production of IL-1β, TNF-α, IL-6 and HMGB1 by LPS-activated RAW264.7 cells. Moreover, Sch B suppressed expression of iNOS, reduced production of PGE2, blocked expression of MyD88 and TLR4, suppressed the activity of NF-κB and decreased phosphorylation of MAPKs in LPS-activated RAW264...
2018: American Journal of Translational Research
Xiaofeng Wu, Lei Cao, Fan Li, Chao Ma, Guangwang Liu, Qiugen Wang
As a main cause of morbidity in the aged population, osteoarthritis (OA) is characterized by cartilage destruction, synovium inflammation, osteophytes, and subchondral bone sclerosis. To date its etiology remains elusive. Recent data highlight an important role of subchondral bone in the onset and progression of OA. Therefore, elucidating the mechanisms underlying abnormal subchondral bone could be of importance in the treatment of OA. Interleukin-6 is a proinflammatory cytokine involved in many physiological and pathological processes...
2018: American Journal of Translational Research
Hao Chang, Yue-Hong Liu, Li-Li Wang, Ju Wang, Zhi-Hong Zhao, Jun-Feng Qu, Sheng-Fa Wang
OBJECTIVE: MicroRNAs have been found to be deregulated in lung cancers, which play crucial roles in tumorigenesis and progression. FBXW7 and FBXW11, two important F-box proteins of the ubiquitin-proteasome system (UPS), can target multiple substrates for degradation, in order to regulate cell proliferation and survival in cancers. In the present study, we aimed to explore the potential role and regulating mechanism of miR-182 in non-small cell lung cancer (NSCLC). METHODS: MiRNA expression was evaluated by quantitative reverse-transcription polymerase chain reaction (qRT-PCR)...
2018: American Journal of Translational Research
Wei-Feng Yao, Jun-Wei Liu, Dong-Sheng Huang
The primary objective of this study was to investigate the role of miR-200a in cell proliferation and epithelial-mesenchymal transition (EMT) through regulating targeting aspartate-β-hydroxylase (ASPH), which may further affect the activation of ERK/PI3K/Akt pathway. Liver cancer and adjacent tissues were collected from 72 cases of liver cancer patients with surgery in our hospital. In this study, the mRNA expression level of miR-200a was significantly decreased by real-time PCR (RT-PCR) detection. ASPH expressions, however, had an opposite tendency compared to that of miR-200a...
2018: American Journal of Translational Research
Jingguo Wu, Yang Yang, Nan Xun, Lijin Zeng, Zhenyu Li, Wen Yang, Yanbing Liang, Zhongfu Ma, Hao Tang
This study was performed to evaluate the cardioprotective effects of osthole (OST) in a rat model of myocardial ischemia/reperfusion injury (MI/RI) and the underlying mechanism. We exposed rat hearts to left anterior descending coronary artery ligation for 30 min followed by 24 h of reperfusion. The results showed that pretreatment with OST ameliorated MI/RI as evidenced by histopathological examination. Moreover, the terminal deoxynucleotidyl transferase dUTP nick end-labeling assay demonstrated that OST suppressed myocardial apoptosis, which may be related to an increase in the Bcl-2/Bax ratio and inhibition of caspase-3 and caspase-9 activation...
2018: American Journal of Translational Research
Zhouhui Ren, Tong Yang, Jie Ding, Weihong Liu, Xiangyu Meng, Pingping Zhang, Kaitai Liu, Ping Wang
MicroRNAs (miRNAs) play an important role in human tumorigenesis as oncogenes or tumor suppressors by directly binding to the 3'-untranslated region of their target mRNAs. MiR-520d-3p has been reported as a tumor suppressor gene in ovarian cancer and gastric cancer, while the function of miR-520d-3p in human breast cancers is still uninvolved. In this study, we initially identified that the expression of miR-520d-3p was significantly reduced in breast cancer specimens and cell lines. The restoration of miR-520d-3p expression not only reduced breast cancer cell viability by causing the accumulation of G2 phase and cell apoptosis, but also inhibited tumorigenicity in vivo ...
2018: American Journal of Translational Research
Yong Liu, Jie Kong, Yong-Ming Xi, Tao Yu, Xiao-Lin Wu, You-Gu Hu
OBJECTIVE: This study aims to investigate the effects of co-transfection of the genes for connective tissue growth factor (CTGF) and tissue inhibitor of metalloproteinase-1 (TIMP1) mediated by adeno-associated virus 2 (AAV2) on degenerative lumbar intervertebral discs in a primate model. METHODS: Twelve 4-7 year-old rhesus monkeys weighing 4.5-7.0 kg were utilized. CTGF and TIMP1 genes carried by AAV2 were injected into the degenerative lumbar intervertebral discs...
2018: American Journal of Translational Research
Pengxun Han, Mumin Shao, Lan Guo, Wenjing Wang, Gaofeng Song, Xuewen Yu, Chunlei Zhang, Na Ge, Tiegang Yi, Shunmin Li, Heng Du, Huili Sun
Diabetes and its renal complications are major medical challenges worldwide. There are no effective drugs currently available for treating diabetes and diabetic kidney disease (DKD), especially in type 1 diabetes (T1D). Evidence has suggested that niclosamide ethanolamine salt (NEN) could improve diabetic symptoms in mice of type 2 diabetes (T2D). However, its role in T1D and DKD has not been studied to date. Here we report that NEN could protect against diabetes in streptozotocin (STZ) induced T1D mice. It increased serum insulin levels, corrected the unbalanced ratio of α-cells to β-cells, and induced islet morphologic changes under diabetic conditions...
2018: American Journal of Translational Research
Chia-Lo Chang, Pei-Hsun Sung, Kuan-Hung Chen, Pei-Lin Shao, Chih-Chao Yang, Ben-Chung Cheng, Kun-Chen Lin, Chih-Hung Chen, Han-Tan Chai, Hsueh-Wen Chang, Hon-Kan Yip, Hong-Hwa Chen
This study tested the hypothesis that healthy adipose-derived mesenchymal stem cell (ADMSC)-derived exosomes (HMSCEXO ) and apoptotic (A) (induced by 12 h hypoxia/12 h starvation)-ADMSC-derived exosomes (AMSCEXO ) were comparably effective at alleviating sepsis syndrome [SS; induced by cecal-ligation and puncture (CLP)]-induced systemic inflammation and reduced organ damage and unfavorable outcomes in rats. SD rats were divided into sham control (SC), SS only, SS + HMSCEXO (100 µg intravenous administration 3 h after CLP), and AMSCEXO ...
2018: American Journal of Translational Research
Yen-Ta Chen, Kuan-Hung Chen, Pei-Hsun Sung, Chih-Chao Yang, Ben-Chung Cheng, Chih-Hung Chen, Chia-Lo Chang, Jiunn-Jye Sheu, Fan-Yen Lee, Pei-Lin Shao, Cheuk-Kwan Sun, Hon-Kan Yip
This study tested the hypothesis that extracorporeal shock wave (ECSW) treatment can effectively inhibit radiation-induced chronic cystitis (CC). Adult male Sprague-Dawley (SD) rats (n = 24) were randomly divided into group 1 (normal control), group 2 (CC induced by radiation with 300 cGy twice with a four-hour interval to the urinary bladder), group 3 [CC with ECSW treatment (0.2 mJ/mm2 /120 impulses/at days 1, 7, and 14 after radiation)]. Bladder specimens were harvested by day 28 after radiation. By day 28 after radiation, the degree of detrusor contraction impairment was significantly higher in group 2 than that in groups 1 and 3, and significantly higher in group 3 than that in group 1 (P<0...
2018: American Journal of Translational Research
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