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American Journal of Translational Research

Xiao-Fei Li, Yan Wang, Dong-Dong Zheng, Hai-Xia Xu, Teng Wang, Min Pan, Jia-Hai Shi, Jian-Hua Zhu
OBJECTIVE: The identification of the biological function of M1 macrophages and the mechanism underlying their role in valvular interstitial cell (VIC) calcification may provide therapeutic targets for the prevention of aortic valve calcification (AVC). This study investigated the mechanism by which M1 macrophages and macrophage-derived microvesicles (MVs) affected the calcification of VICs. An additional aim was to investigate the involvement of the miR-214 pathway in this process. METHODS: The M1 or M2 macrophage phenotype in human calcific aortic valve was confirmed by gene expression analysis of M1 or M2 macrophage markers...
2016: American Journal of Translational Research
Xiang-Qian Luo, Shao-Bo Yang, Shu-Qi Qiu, Rui-Di Xie, Li-Tao Yang, Yu-Xing Ke, Hong-Xia Zhao, Xiao-Rui Geng, Gui Yang, Zhi-Qiang Liu, Jiang-Qi Liu, Shuai Wang, Da-Bo Liu, Jun Liu
The dysfunction of peripheral immune tolerance plays an important role in the pathogenesis of allergic diseases. Recent reports indicate that micro RNA (miR)-98 is associated with the process of aberrant immune responses. This study aims to test a hypothesis that miR-98 is associated with the pathogenesis of airway allergy via interfering with the development of regulatory B cells (Breg). In this study, patients with airway allergy were recruited into this study. The frequency of Bregs was assessed by flow cytometry...
2016: American Journal of Translational Research
Patrycja Sosińska, Ewa Baum, Beata Maćkowiak, Ryszard Staniszewski, Tomasz Jasinski, Kazuo Umezawa, Andrzej Bręborowicz
Peritoneal mesothelial cells exposed to bioincompatible dialysis fluids contribute to damage of the peritoneum during chronic dialysis. Inflammatory response triggered in the mesothelium leading to neovascularization and fibrosis plays an important role in that process. We studied the effects of Dehydroxymethyepoxyquinmicin (DHMEQ)-an NF-κB inhibitor on function of human peritoneal mesothelial cells (HPMC) in in vitro culture. DHMEQ studied in concentrations of 1-10 µg/ml was not toxic to HPMC. Synthesis of IL-6, MCP-1 and hyaluronan in unstimulated and stimulated with interleukin-1 (100 pg/ml) HPMC was inhibited in the presence of DHMEQ and the effect was proportional to the dose of the drug...
2016: American Journal of Translational Research
Jiapeng Liu, Wei Dong, Tao Wang, Liang Liu, Long Zhan, Yifei Shi, Jiange Han
OBJECTIVE: To investigate the effects of etomidate and propofol on immune function in patients with lung adenocarcinoma. METHODS: Sixty patients who were scheduled for lung cancer surgery under general anesthesia were studied. The patients were randomly divided into an etomidate total intravenous anesthesia group (group E) and a propofol total intravenous anesthesia group (group P), with 30 cases in each group. RESULTS: Within group comparison: The percentage of CD4+ in the two groups was significantly reduced at 24 hours post-operation (T2) compared with the percentage before surgery, whereas the percentage of CD8+ was higher at T2...
2016: American Journal of Translational Research
Yong Dai, Jinbo Jiang, Yanlei Wang, Zutao Jin, Sanyuan Hu
As the most common malignant tumor, gastric cancer had persistently high occurrence and mortality rate worldwide. Unfavorable treating outcome occur due to distal metastasis, making the inhibition of angiogenesis and managing tumor metastasis being crucial factors for affecting prognosis. Vascular endothelial growth factor-C (VEGF-C) is one important angiogenesis factor and mainly facilitates proliferation and differentiation of vascular endothelial cells in angiogenesis. It has been indicated in development and occurrence in gastric cancer, while its expression and correlation with microvascular density (MVD)/lymph node metastasis are still unclear...
2016: American Journal of Translational Research
Jianzhong Wu, Shuchun Li, Rong Ma, Ashok Sharma, Shan Bai, Boying Dun, Haixia Cao, Changwen Jing, Jinxiong She, Jifeng Feng
The expression of a number of genes can influence the response rates to chemotherapy while genes encoding receptor tyrosine kinases (RTKs) determine the response to most targeted cancer therapies currently used in clinics. In this study, we evaluated seven genes known to influence chemotherapeutic response (ERCC1, BRCA1, RRM1, TUBB3, STMN1, TYMS, and TOP2A) and five RTKs (EGFR, ERBB2, PDGFRB, VEGFR1 and VEGFR2) in non-small cell lung cancer (NSCLC) and esophagus cancer (EC) and the data are compared to gastric cancer (GC) data reported previously...
2016: American Journal of Translational Research
Ken Zhao, Zhen Chen, Xu-Dong Lv, Gang Dong, Huan Xia
OBJECTIVE: This study aims to explore the impact of micro RNA miR-145 on retinal pigment epithelial cell proliferation and apoptosis. METHODS: A stable culture and passage system of hPNE cells was first established, and its migration ability was determined. Then, miR-145 lentiviral vectors were constructed to transfect hPRE cells. Thereafter, hRPE cell proliferation was detected by MTT assay after they were transfected by lentivirus, cell cycle was analyzed by flow cytometry, and apoptosis was detected by Annexin V/PI double staining immunofluorescence...
2016: American Journal of Translational Research
Zhen-Shi Ye, Li-Na Fan, Lin Wang, Bin Yang, Li-Feng Wang, Yuan-Sheng Liu, Su-Na Ji, Hong-Zhi Xu, Chuan-Xing Xiao
The pathogenesis of colon cancer (Cca) is to be further investigated. Vitamin D deficiency is associated with cancer growth; the underlying mechanism is unclear. Published data indicate that Cca cells express CD23. This study tests a hypothesis that exposure to IgE induces Cca cell apoptosis. In this study, the effect of ligation of CD23 by IgE on the expression of cyp27b1 was performed with Cca cells. The induction of apoptosis of Cca cells by IgE was assessed in a cell culture model. We observed that Cca cells express CD23; ligation of CD23 with IgE on Cca cells increased the expression of cyp27b1 in Cca, which promoted the conversion of VD3 to calcitriol, the latter increased the expression of FasL by Cca cells, and induced apoptosis of Cca cells...
2016: American Journal of Translational Research
Markus Winny, Lavinia Maegel, Leonie Victoria Grethe, Danny Jonigk, Paul Borchert, Alexander Kaltenborn, Harald Schrem, Juergen Klempnauer, Daniel Poehnert
BACKGROUND: Intraperitoneal onlay meshes (IPOM) can be associated with intestine-to-mesh adhesion formation, implementing risks like pain, enterocutaneous fistula, infection, and female infertility. This study investigates, whether a treatment of impaired intestinum with the anti-adhesive and hemostyptic agent 4DryField(®) PH prevents adhesion formation. METHODS: In 20 male LEWIS rats uncoated polypropylene meshes were sewn to the inner abdominal wall and the cecum of the respective animal was de-peritonealized by peritoneal abrasion by a gauze swap, and meso-sutures ensured a constant contact of injured areas...
2016: American Journal of Translational Research
Xin-Yu Ge, Shang-Ping Fang, Miao Zhou, Jing Luo, Juan Wei, Xue-Ping Wen, Xiao-Di Yan, Zui Zou
Sepsis, the most severe manifestation of infection, poses a major challenge to health-care systems around the world. Limited ability to clean and remove the pathogen renders difficulty in septic patients to recover from the phase of immunoparalysis. The present study found the vital role of CX3CR1 internalization on sepsis-induced immunoparalysis. A mouse model with cecal ligation and puncture (CLP) and cell model with lipopolysaccharides (LPS) were employed to explore the relationship between CX3CR1 internalization and septic immunoparalysis...
2016: American Journal of Translational Research
Yuan-Li Chen, Guo Xu, Xiao Liang, Juan Wei, Jing Luo, Guan-Nan Chen, Xiao-Di Yan, Xue-Ping Wen, Ming Zhong, Xin Lv
Pyroptosis is a programmed cell death associated with caspase-1 and accompanied by the secretion of a large number of pro-inflammatory cytokines. In the acute stage of sepsis, the release of several pro-inflammatory cytokines aggravates hepatic cell death, and acute liver injury is aggravated with the progress of the disease, resulting in acute liver failure with a very high mortality rate. The present study investigated the effect of inhibiting hepatic cell pyroptosis on the septic acute liver injury. Septic acute liver injury mice model was established by cecal ligation and puncture (CLP model)...
2016: American Journal of Translational Research
Isabel Moreno-Indias, Lidia Sánchez-Alcoholado, Eduardo García-Fuentes, Fernando Cardona, Maria Isabel Queipo-Ortuño, Francisco J Tinahones
Alterations in intestinal microbiota composition could promote a proinflammatory state in adipose tissue that is associated with obesity and insulin resistance. Our aim was to identify the gut microbiota associated with insulin resistance in appendix samples from morbidly obese patients classified in 2 groups, high (IR-MO) and low insulin-resistant (NIR-MO), and to determine the possible association between these gut microbiota and variables associated with insulin resistance and the expression of genes related to inflammation and macrophage infiltration in adipose tissue...
2016: American Journal of Translational Research
Hongrong Xie, Hui Hu, Ming Chang, Dongya Huang, Xiaobo Gu, Xinli Xiong, Ran Xiong, Linsen Hu, Gang Li
Parkinson's disease (PD) is characterized by the pathological accumulation of misfolded proteins. Molecular chaperones assist in the proper folding of proteins and removal of irreversibly misfolded proteins. This study aims to identify potential chaperones associated with protein misfolding and accumulation in PD. ATRA/TPA-differentiated SH-SY5Y cells were treated with 1 mM of MPP(+) for 48 hours. Proteins were analyzed by 2D-DIGE followed by MALDI-ToF MS. The treatment of differentiated SH-SY5Y cells by MPP(+) led to the unambiguous identification of 10 protein spots, which corresponds to six proteins...
2016: American Journal of Translational Research
Nengwang Yu, Shuai Fu, Junwen Hao, Aimin Zhang, Zhihou Fu
The mouse is an optimal animal model for kidney transplantation. Recent reports suggest that application of poloxamer 407, a thermosensitive in situ gel, during the sutureless technique significantly increases animal survival, compared to traditional methods. However, further improvement of this technology is greatly needed but remains unexplored. Here, we detected significant inflammation at the region of ureter anastomosis, after kidney transplantation using poloxamer 407. Since chemokines play a pivotal role during inflammation, we implanted an Alzet osmotic pump that gradually releases AMD3100 (a specific inhibitor of the binding of stromal cell-derived factor 1 (SDF-1) to its receptor, CXCR4) at the site of ureter anastomosis in mice that had undergone kidney transplantation...
2016: American Journal of Translational Research
Ka Wu, Chao Guo, Xiuli Lu, Xinmou Wu, Hongmei Pan, Min Su
Acetaminophen (APAP), an over the counter (OTC) medication, is widely used in antipyretic treatment. Although the risk of dose-dependent cytotoxicity has been known, the potential effect of perinatal exposure to acetaminophen on metabolic function in offspring remains uninvestigated. Therefore, we established a prenatally APAP-exposed pregnancy mouse model to assess the possible adverse effect on liver metabolic function in offspring. Biochemical assays were applied in analysis of basic metabolic parameters in postnatal mice...
2016: American Journal of Translational Research
An-Chen Guo, Takho Chu, Xu-Qing Liu, Huan-Xing Su, Wu-Tian Wu
Multiple sclerosis (MS) is ademyelinating disease in the central nervous system (CNS). Majority of the MS patients show relapsing-remitting disease course. Evidences show that oligodendrocyte precursor cells (OPCs), which remain relatively quiescent in normal adult CNS, play a key role in the remitting phase by proliferation and remyelination. In the present study, we found that spinal cord astrocytesco-expressed progenitor cell marker and oligodendroglial lineage markers in the remittance phase in adult rat experimental autoimmune encephalomyelitis (EAE) model...
2016: American Journal of Translational Research
Ning Zhao, Yanjuan Liu, Hongfeng Liang, Xuejun Jiang
OBJECT: To determine the potential of bone marrow-derived mesenchymal stem cells (BMSCs) for immunomodulatory mechanism in mice model of allergic rhinitis (AR). METHODS: BMSCs were isolated and the surface markers and stemness were analyzed. The effect of BMSCs was evaluated in BALB/c mice that were randomly divided into three groups (control group, ovalbumin (OVA) group, OVA+BMSCs group). BMSCs were administered intravenously to OVA sensitized mice on days 1, 7, 14 and 21, and subsequent OVA challenge was conducted daily from days 22 to 35...
2016: American Journal of Translational Research
Yongwei Li, Lu Chen, Chunxia Wang, Jianshe Chen, Xiaoqian Zhang, Yue Hu, Xiaobin Niu, Dongxu Pei, Zhiqiang He, Yongyi Bi
This study aims to explore the role of extra-cellular matrix metalloproteinase inducer (EMMPRIN) in the drug resistance of the pseudomonas aeruginosa (PA). The BALB/c mice were transfected with PA, then the mice were infected with the siRNA of EMMPRIN to silence the EMMPRIN gene. The EMMPRIN mRNA and protein were detected by using RT-PCR and western blot, respectively. In order to examine the function of EMMPRIN in drug resistance of PA, the BALB/c and C57BL/6 mice were treated with EMMPRIN siRNA. The cytokines, EMMPRIN and MMP9 were examined by the RP-PCR and ELISA, respectively, undergoing the silence of EMMPRIN siRNA...
2016: American Journal of Translational Research
Xuhong Zhou, Fei Zhuang, Hong Li, Kun Zheng, Ze Hong, Weijing Feng, Wendi Zhou, Jian Chen
Epilepsy is linked to mutations in KCNQ channels. KCNQ channels including KCNQ2 and KCNQ3 are enriched in neurons, regulating action potential generation and modulation. Here, we showed that properties of KCNQ2 channel in rat hippocampal cultured neurons are regulated by ubiquitous calcium sensor calmodulin. We analyzed calmodulin function on the KCNQ2 channel in both HEK293 cells and neurons. We used shRNAs to suppress expression of calmodulin protein. On the other hand, we used cDNA to over-express calmodulin in HEK293 and neuron cells...
2016: American Journal of Translational Research
Jinman Zhong, Weiwei Zhao, Fang Ren, Shun Qi, Xifu Wang, Tianchu Lv, Zhanliang Su, Hong Yin, Jing Ren, Yi Huan
The aim of the study was to investigate the value of computed tomography (CT), magnetic resonance imaging (MRI) and diffusion weighted imaging (DWI) for diagnosing lymph nodes metastasis before treatment in gastric cancer. Eighty-two patients with proven gastric cancer underwent CT, morphological MRI (T2WI) and DWI examinations. Two radiologists independently assessed these images for the presence of lymph nodes involvement. Pathologic findings were considered as "gold standard". Independent samples t-test was used for the comparisons of short diameters and ADC values between the positive lymph nodes and the negative lymph nodes...
2016: American Journal of Translational Research
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