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Probiotics and Antimicrobial Proteins

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https://www.readbyqxmd.com/read/28434154/protective-effects-of-cell-free-supernatant-and-live-lactic-acid-bacteria-isolated-from-thai-pigs-against-a-pandemic-strain-of-porcine-epidemic-diarrhea-virus
#1
Wandee Sirichokchatchawan, Gun Temeeyasen, Dachrit Nilubol, Nuvee Prapasarakul
Porcine epidemic diarrhea virus (PEDV) is a coronavirus which causes severe diarrhea and fatal dehydration in piglets. In general, probiotic supplements could enhance recovery and protect piglets against enteric pathogens. Seven local lactic acid bacteria (LAB), (Ent. faecium 79N and 40N, Lact. plantarum 22F, 25F and 31F, Ped. acidilactici 72N and Ped. pentosaceus 77F) from pig feces were well-characterized as high potential probiotics. Cell-free supernatants (CFS) and live LAB were evaluated for antiviral activities by co-incubation on Vero cells and challenged with a pandemic strain of PEDV isolated from pigs in Thailand...
April 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28432677/development-of-yoghurt-with-ju%C3%A3-ara-pulp-euterpe-edulis-m-and-the-probiotic-lactobacillus-acidophilus-la5
#2
Marina V Geraldi, Fabricio L Tulini, Vanessa M Souza, Elaine C P De Martinis
Yoghurts are dairy products consumed worldwide and can be supplemented with substances that provide extra health benefits as well as probiotic strains. In this context, the present study aimed to prepare a yoghurt added of juçara (Euterpe edulis M.) pulp and the commercial probiotic strain Lactobacillus acidophilus La5. Moreover, the probiotic survival during storage and after in vitro exposure to simulated gastric and enteric conditions was evaluated. Four formulations of yoghurt were prepared: (a) natural yoghurt, (b) yoghurt added of probiotic, (c) yoghurt added of juçara pulp, and (d) yoghurt added of probiotic culture and juçara pulp...
April 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28432676/bifidobacterium-adolescentis-dsm-20083-and-lactobacillus-casei-lafti-l26-dsl-probiotics-able-to-block-the-in-vitro-adherence-of-rotavirus-in-ma104-cells
#3
Karem Prunella Fernandez-Duarte, Nury Nathalia Olaya-Galán, Sandra Patricia Salas-Cárdenas, Jazmin Lopez-Rozo, Maria Fernanda Gutierrez-Fernandez
Rotavirus is the leading worldwide cause of gastroenteritis in children under five years of age. Even though there are some available vaccines to prevent the disease, there are limited strategies for challenging diarrhea induced by rotavirus infection. For this reason, researchers are constantly searching for other approaches to control diarrhea by means of probiotics. In order to demonstrate the ability of some probiotic bacteria to interfere with the in vitro rotavirus infection in MA104 cells, strains of Lactobacillus sp...
April 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28421423/the-effects-of-direct-fed-microbial-supplementation-as-an-alternative-to-antibiotics-on-growth-performance-intestinal-immune-status-and-epithelial-barrier-gene-expression-in-broiler-chickens
#4
U Gadde, S T Oh, Y S Lee, E Davis, N Zimmerman, T Rehberger, Hyun S Lillehoj
The objective of this study was to investigate the effects of Bacillus subtilis-based probiotic supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine, and tight junction (TJ) protein mRNA expression. Zero-day-old broiler chicks (n = 140) were randomly assigned to one of five dietary treatments: basal diet (CON); basal diet supplemented with either antibiotic bacitracin methylene disalicylate (BMD); or probiotics, namely, B. subtilis strain 1781 (PB1), a combination of B...
April 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28417292/probiotic-strawberry-yogurts-microbiological-chemical-and-sensory-properties
#5
Tamer Turgut, Songül Cakmakci
This study was performed to determine the viability of Lactobacillus acidophilus and Bifidobacterium bifidum in yogurt made with strawberry marmalade (SM) and to examine the quality properties of probiotic yogurt. Acidity, pH, bacterial counts and sensory analysis of the yogurt samples were investigated on days 1, 3, 5, 7, 10 and 14 during storage at 4 °C. The survival rate of L. acidophilus was greater than that of B. bifidum. The viability of L. acidophilus decreased during the storage period, but B. bifidum numbers remained stable during the storage period...
April 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28401477/a-recombinant-probiotic-lactobacillus-casei-expressing-the-clostridium-perfringens-%C3%AE-toxoid-as-an-orally-vaccine-candidate-against-gas-gangrene-and-necrotic-enteritis
#6
Mojtaba Alimolaei, Mehdi Golchin, Jalil Abshenas, Majid Ezatkhah, Mehrdad Shamsaddini Bafti
The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-α strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy...
April 11, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28374172/evaluation-of-potential-probiotics-isolated-from-human-milk-and-colostrum
#7
Quésia S Damaceno, Jaqueline P Souza, Jacques R Nicoli, Raquel L Paula, Gabriela B Assis, Henrique C Figueiredo, Vasco Azevedo, Flaviano S Martins
Several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. The present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. Milk samples were collected from 47 healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. Bacterial isolates were counted and identified by Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight mass spectrometry and then tested for probiotic properties...
April 3, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28361445/roles-of-probiotic-lactobacilli-inclusion-in-helping-piglets-establish-healthy-intestinal-inter-environment-for-pathogen-defense
#8
REVIEW
Jiajun Yang, Kun Qian, Chonglong Wang, Yijing Wu
The gastrointestinal tract of pigs is densely populated with microorganisms that closely interact with the host and with ingested feed. Gut microbiota benefits the host by providing nutrients from dietary substrates and modulating the development and function of the digestive and immune systems. An optimized gastrointestinal microbiome is crucial for pigs' health, and establishment of the microbiome in piglets is especially important for growth and disease resistance. However, the microbiome in the gastrointestinal tract of piglets is immature and easily influenced by the environment...
March 30, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28357646/the-usefulness-of-non-toxic-plant-metabolites-in-the-control-of-bacterial-proliferation
#9
Sergio Gutiérrez, Alfredo Morán, Honorina Martínez-Blanco, Miguel A Ferrero, Leandro B Rodríguez-Aparicio
The effect of generally recognised as safe (GRAS) plant metabolites in regulating the growth of human pathogenic and probiotic bacteria and in the formation of biofilm was investigated. Thymol, carvacrol and eugenol showed the strongest antibacterial action against both pathogenic and probiotic microorganisms, at a subinhibitory concentration (SIC) of ≤50 μg ml(-1). Genistein, hydroquinone, p-hydroxybenzoic acid and resveratrol also showed antibacterial effects but at a wide concentration range (SIC = 50-1000 μg ml(-1))...
March 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28342109/sensitivity-to-enterocins-of-biogenic-amine-producing-faecal-enterococci-from-ostriches-and-pheasants
#10
Andrea Lauková, Anna Kandričáková, Leona Buňková, Pavel Pleva, Jana Ščerbová
Enterococci are widespread bacteria forming the third largest genus among lactic acid bacteria. Some possess probiotic properties or they can produce beneficial proteinaceous antimicrobial substances called enterocins. On the other hand, some enterococci produce biogenic amines (BAs), so this study is focused on the sensitivity to enterocins of biogenic amine-producing faecal enterococci from ostriches and pheasants. Altogether, 60 enterococci isolated from faeces of ostriches and pheasants were tested for production of BAs...
March 24, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28321827/effects-of-dietary-administration-of-shewanella-xiamenensis-a-1-aeromonas-veronii-a-7-and-bacillus-subtilis-single-or-combined-on-the-grass-carp-ctenopharyngodon-idella-intestinal-microbiota
#11
Kai Hao, Zhuo-Qi Wu, Dong-Liang Li, Xiao-Bo Yu, Gao-Xue Wang, Fei Ling
Gut microbiota of grass carp plays an important role in host. However, detailed information regarding the changes of microbiota after probiotics administration in relation to the gastrointestinal microbiota is absent. In the present study, dietary administration of putative probiotics Shewanella xiamenensis A-1, Aeromonas veronii A-7, and Bacillus subtilisstrains was conducted in grass carp to investigate if there is a discernible alteration in intestinal microbiota and whether the alteration is associated with previous study about the immunity regulation in grass carp...
March 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28321826/the-role-of-probiotics-in-the-treatment-of-dysentery-a-randomized-double-blind-clinical-trial
#12
Alireza Sharif, Hamed Haddad Kashani, Elahe Nasri, Zahra Soleimani, Mohammad Reza Sharif
Diarrhea is considered as an important cause of morbidity and mortality, even though one of the main reasons of death following diarrhea is initiated by dysentery. In recent years, the consumption of probiotics has been proposed for the treatment of infectious diarrhea. Despite most of the studies on probiotics have focused on acute watery diarrhea, few studies in the field of dysentery have found beneficial effects of probiotics. This study is a randomized double-blind clinical trial. The patients were randomly placed into control and case groups...
March 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28321825/effect-of-feeding-soy-milk-fermented-by-probiotic-bacteria-on-some-blood-criteria-and-weight-of-experimental-animals
#13
Alaa Kareem Niamah, Ali Ahmed Sahi, Alaa S N Al-Sharifi
The chemical composition of soy milk fermented by probiotic starter (Log.11.1 CFU/ml) which consists of Streptococcus thermophilus, Lactobacillus acidophilus LA-5, and Bifidobacterium bifidum Bb-12 was studied. The percentage of each moisture, protein, fat, ash, carbohydrate and total acidity were 88.8, 4.2, 2.3, 0.82, 4.6, and 0.83, respectively, and the phytic acid rate was 0.075% after fermentation. The experimental animals were fed with 3 mL and 2 mL of soy milk fermented for 40 days. The result showed decreasing the rate of cholesterol and triglyceride in T1 treatment (3 mL of soy milk fermented) which were (114...
March 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28316010/protective-effect-of-lactobacillus-casei-on-dmh-induced-colon-carcinogenesis-in-mice
#14
Cesar Antonio Irecta-Nájera, María Del Rosario Huizar-López, Josefina Casas-Solís, Patricia Castro-Félix, Anne Santerre
The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation...
March 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28303478/effects-of-synbiotic2000%C3%A2-forte-on-the-intestinal-motility-and-interstitial-cells-of-cajal-in-tbi-mouse-model
#15
Limei Zhang, Jing Zeng, Yuanyuan Ma, Min Tan, Min Zhou, Huan Fang, Stig Bengmark, Jingci Zhu
The main objective of this study was to investigate the effects of Synbiotic2000™ Forte on the intestinal motility and interstitial cells of Cajal (ICC) in traumatic brain injury (TBI) mouse model. Kunming mice were randomly divided into sham operation group (S group), enteral nutrition group with TBI (E group), and Synbiotic2000™ Forte group with TBI (P group). The contractile activity of the intestinal smooth muscle, densities and ultrastructure of the ICC, kit protein concentration, weight, and defecation of mice were monitored and analyzed...
March 16, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28303477/effect-of-co-overexpression-of-nisin-key-genes-on-nisin-production-improvement-in-lactococcus-lactis-ls01
#16
Zhi-Jian Ni, Xiao-Yuan Zhang, Fei Liu, Miao Wang, Rong-Hua Hao, Pei-Xue Ling, Xi-Qiang Zhu
Nisin is a small antimicrobial peptide produced by several subset strains of Lactococcus lactis. To improve nisin yield in the producer L. lactis LS01, we proposed a successive fusion of nisA with nisRK and nisFEG into a single shuttle expression vector pMG36e under the control of the native strong constitutive promoter p32. Subsequently, the recombinant vectors were transplanted into the producer cell through electroporation. Nisin productivity was determined through sodium dodecyl sulfate-polyacrylamide gel electrophoresis and bioactivity assays...
March 16, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28293865/the-inhibition-effect-of-lactobacilli-against-growth-and-biofilm-formation-of-pseudomonas-aeruginosa
#17
Dariush Shokri, Mohammad Rabbani Khorasgani, Milad Mohkam, Seyed Masih Fatemi, Younes Ghasemi, Asghar Taheri-Kafrani
The emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of Pseudomonas aeruginosa and determination of their inhibition mechanism. Antibiotic susceptibility and classification of acquired resistance profiles of 80 P...
March 14, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28275899/spray-dried-formulation-of-5-fluorouracil-embedded-with-probiotic-biomass-in-vitro-and-in-vivo-studies
#18
Anshul Sharma, Malika Arora, Amit K Goyal, Goutam Rath
The present study is utilizing the targeted therapeutic approach and antioxidant potential of selected probiotic biomass in mitigating toxic side effects of chemotherapeutic agents. Multicomponent carrier system consisting of 5-fluorouracil (5-FU) and selected probiotic strain with higher free radical scavenging activity was prepared using spray drying technique. Prepared spray dried microparticles were characterized for various physical, pharmaceutical, and biopharmaceutical properties including particle size, moisture content, entrapment efficiency, in vitro drug release, DSC, XRD, cell uptake, histopathology, and pharmacokinetic studies...
March 8, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28271469/lactobacillus-plantarum-and-its-probiotic-and-food-potentialities
#19
Hamza Ait Seddik, Farida Bendali, Frédérique Gancel, Ismail Fliss, Giuseppe Spano, Djamel Drider
The number of studies claiming probiotic health effects of Lactobacillus plantarum is escalating. Lb. plantarum is a lactic acid bacterium found in diverse ecological niches, highlighting its particular capabilities of adaptation and genome plasticity. Another function that needs to be underlined is the capabilities of Lb. plantarum to produce diverse and potent bacteriocins, which are antimicrobial peptides with possible applications as food preservative or antibiotic complementary agents. Taken together, all these characteristics design Lb...
March 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28258546/beneficial-and-safety-properties-of-a-corynebacterium-vitaeruminis-strain-isolated-from-the-cow-rumen
#20
M Colombo, N P A Castilho, S D Todorov, L A Nero
Corynebacterium vitaeruminis MRU4 was isolated from the cow rumen and was differentiated from other isolates by rep-PCR and RAPD and identified by 16S rRNA sequencing. This strain presented higher survival rates for low pH and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. C. vitaeruminis MRU4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with Listeria monocytogenes Scott A, 41.6% co-aggregation rate with Enterococcus faecalis ATCC 19443, 10...
March 3, 2017: Probiotics and Antimicrobial Proteins
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