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Probiotics and Antimicrobial Proteins

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https://www.readbyqxmd.com/read/28643226/capsular-polysaccharides-of-lactobacillus-spp-theoretical-and-practical-aspects-of-simple-visualization-methods
#1
Magdalena Oleksy, Elżbieta Klewicka
Lactobacillus strains can synthesize capsular polysaccharides (CPS), which are important substances in the dairy industry-they exhibit many important technological as well as health-promoting properties. Technological advancements have made it possible to detect bacterial capsules using costly and labor-intensive methods, such as serological reactions, molecular genetic techniques, and electron microscopy. Light microscopy, which is the method of interest in this paper, is one of the most widely accessible and cheapest techniques...
June 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28643225/using-illumina-based-sequence-analysis-to-guide-probiotic-candidate-selection-and-isolation
#2
Wenfeng Wang, Yi Li, Wangsen Qin, Changyi Sun, Hongming Tan, Lixiang Cao
Selection for probiotic candidates by in vivo experimental trials is time and labor consuming; more informed strategy is needed to select successful probiotic candidates. The aim of the study was to elucidate the microbial taxa transmitted from maize seeds to seedlings during the germination process of maize and their probiotic effects. The bacterial and fungal taxa in kernel germs and sprouts were analyzed by Illumina-based sequencing. The sprouts contained more diverse fungi than those in germs. The bacterial species (OTUs) declined with the germination from germs to the sprouts...
June 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28639176/combined-or-individual-effects-of-dietary-probiotic-pedicoccus-acidilactici-and-nucleotide-on-growth-performance-intestinal-microbiota-hemato-biochemical-parameters-and-innate-immune-response-in-goldfish-carassius-auratus
#3
Nooshin Mehdinejad, Mohammad Reza Imanpour, Valiollah Jafari
This study investigated the effect of dietary supplementation of probiotic Pedicoccus acidilactici and nucleotide (separately or combined) on growth performance, intestinal microbiota, hemato-immunological parameters, and immunity response in goldfish (Carassius auratus). Fish (average weight 5-6 g) were acclimatized and divided into eight experimental diets supplemented with P. acidilactici of different concentrations (0.1, 0.2, and 0.3% diet) and nucleotides (0 and 0.5% diet) for 6 months. Fish fed with experimental diets showed significant differences in terms of final weight, weight gain, feed conversion ratio, daily growth rate, and condition factor when compared to control diet (P < 0...
June 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28634812/semi-industrial-scale-production-of-a-new-yeast-with-probiotic-traits-cryptococcus-sp-ymhs-isolated-from-the-red-sea
#4
Ashraf F El-Baz, Hesham A El-Enshasy, Yousseria M Shetaia, Hoda Mahrous, Nor Zalina Othman, Ahmed E Yousef
A new yeast strain with promising probiotic traits was isolated from the Red Sea water samples. The isolate (YMHS) was subjected to genetic characterization and identified as Cryptococcus sp. Nucleotide sequence analysis of the rRNA gene internal transcribed spacer regions showed 95% sequence similarity between the isolate and Cryptococcus albidus. Cryptococcus sp. YMHS exhibited desirable characteristics of probiotic microorganisms; it has tolerance to low pH in simulated gastric juice, resistance to bile salts, hydrophobic characteristics, broad antimicrobial activity, and in vitro ability to degrade cholesterol...
June 20, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28631250/lactobacillus-salivarius-nk02-a-potent-probiotic-for-clinical-application-in-mouthwash
#5
Neda Sajedinejad, Mojgan Paknejad, Behzad Houshmand, Hakimeh Sharafi, Reza Jelodar, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi
A specific strain of naturally occurring oral lactobacilli was isolated and identified based on morphological, biochemical, and 16S rRNA gene sequencing. The phylogenetic affiliation of the isolate confirmed that the NK02 strain had close association with the Lactobacillus salivarius. An effective mouthwash was developed for treatment of periodontitis and suppression of the indicator bacterium Aggregatibacter actinomycetemcomitans which is an obvious pathogen of periodontal disease. The mouthwash containing L...
June 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28631249/molecular-characterisation-of-a-novel-isoform-of-hepatic-antimicrobial-peptide-hepcidin-le-hepc-from-leiognathus-equulus-and-analysis-of-its-functional-properties-in-silico
#6
Aishwarya Nair, K S Sruthy, E R Chaithanya, T P Sajeevan, I S Bright Singh, Rosamma Philip
Hepcidin represents a family of cysteine-rich antimicrobial peptides that are mainly expressed in the liver of living organisms. In this study, we have identified and characterised a novel isoform of hepcidin from the common pony fish, Leiognathus equulus (Le-Hepc). A 261-bp fragment cDNA coding for 86 amino acids was obtained. Homologous analysis showed that Le-Hepc belongs to the hepcidin super family and shares sequence identity with other known fish pre-propeptide hepcidin sequences. The ORF encodes for a 24-amino acid (aa) signal peptide coupled to a 36-aa prodomain followed by a 26-aa mature peptide...
June 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28585158/properties-of-probiotics-kocuria-sm1-and-rhodococcus-sm2-isolated-from-fish-guts
#7
S M Sharifuzzaman, Hafizur Rahman, Dawn A Austin, B Austin
This study characterized probiotics Kocuria SM1 and Rhodococcus SM2, which were recovered from the intestinal microbiota of rainbow trout (Oncorhynchus mykiss, Walbaum). The cultures were Gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. Cell multiplication of SM1 and SM2 was observed at 4-37 °C (45 °C for SM1), in 0-20% (w/v) NaCl and at pH 2-11. The viability was not affected when exposed to pepsin at pH 2.0 and 3.0, and pancreatin at pH 8.0. Neither isolates were chrome azurol S-positive for siderophore production...
June 5, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28578494/colonization-and-gut-flora-modulation-of-lactobacillus-kefiranofaciens-zw3-in-the-intestinal-tract-of-mice
#8
Zhuqing Xing, Wei Tang, Ying Yang, Weitao Geng, Rizwan Ur Rehman, Yanping Wang
This study evaluated the distribution and colonization of Lactobacillus kefiranofaciens ZW3 and determined its capacity to modulate the gut microbiota in an animal model. Based on (1) fluorescence imaging, (2) flow cytometry, and (3) qPCR, we found that ZW3 successfully adhered to mouse mucous tissue and colonized the mouse ileum. Gut microbiota profiling was performed using high-throughput sequencing. After continuous intubation with ZW3 for 1 week, the proportion of Lachnospiraceae, a family of butyric acid-producing bacteria, increased at day 7 (11...
June 3, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28573492/bactericidal-activity-and-synergy-studies-of-peptide-ap-cect7121-against-multi-resistant-bacteria-isolated-from-human-and-animal-soft-tissue-infections
#9
Gastón Delpech, Mariana Bistoletti, Mónica Ceci, Sabina Lissarrague, Sergio Sánchez Bruni, Mónica Sparo
AP-CECT7121 is an antimicrobial peptide, produced by Enterococcus faecalis CECT7121, with bactericidal activity against Gram-positive bacteria. The aim of this study was to evaluate the bactericidal activity of AP-CECT7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft tissue infections. During the period 2014-2015, bacterial strains producing human and animal soft tissue infections were studied. Samples from patients attended at a general hospital and cattle from four dairies in the Province of Buenos Aires (Argentina) were included...
June 1, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28560515/stimulating-effects-of-sucrose-and-inulin-on-growth-lactate-and-bacteriocin-productions-by-pediococcus-pentosaceus
#10
Pamela Oliveira de Souza de Azevedo, Attilio Converti, José Manuel Domínguez, Ricardo Pinheiro de Souza Oliveira
Sucrose and inulin, when combined with glucose, behaved as stimulating agents of bacteriocin production by Pediococcus pentosaceus ATCC 43200. When such microbial strain was grown in glucose-based Man, Rogosa, and Sharpe (MRS) medium, without any additional supplement, it showed higher maximum cell concentration (2.68 ± 1.10 g/L) and longer generation time (2.17 ± 0.02 h), but lower specific growth rate (0.32 ± 0.01 h(-1)) than in the same medium supplemented with 1.0% of both ingredients (2.53 ± 1...
May 30, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28560514/illumina-based-sequencing-analysis-directed-selection-for-actinobacterial-probiotic-candidates-for-banana-plants
#11
Xin Du, Yanyan Zhai, Qingli Deng, Hongming Tan, Lixiang Cao
As potential probiotic candidates, plant vertically transmitted actinobacteria are beneficial to growth and health of host plants. New methods to isolate the actinobacterial taxa with low growth rates should be developed. Based on the actinobacterial population information, the probiotic actinobacterial taxa could be directly isolated from healthy banana shoot tips. However, actinobacterial DNAs with high GC contents could bias estimates of actinobacteria by PCR. In the study, two amplicon sequencing strategies were adopted to elucidate the endophytic actinobacterial community of banana plants...
May 30, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28555255/a-simple-method-for-the-purification-of-nisin
#12
Ronan Gough, Beatriz Gómez-Sala, Paula M O'Connor, Mary C Rea, Song Miao, Colin Hill, André Brodkorb
Nisin, an antimicrobial peptide showing activity against a broad range of Gram-positive bacteria, is widely used as a food preservative and has potential as a therapeutic for a range of infectious diseases. Here, we present a simple purification method, based on a salting-out approach, which can produce a powder containing ∼33% nisin, from a nisin-producing culture in a whey permeate-based medium. This process removes over 99% of the lactic acid, NaCl, lactose and non-nisin proteins from the cell-free culture supernatant...
May 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28527125/diversity-and-probiotic-potential-of-lactic-acid-bacteria-isolated-from-horreh-a-traditional-iranian-fermented-food
#13
Alireza Vasiee, Behrooz Alizadeh Behbahani, Farideh Tabatabaei Yazdi, Seyed Ali Mortazavi, Hamid Noorbakhsh
The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) strains isolated from Horreh. Some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. A total of 140 Gram-positive and catalase-negative isolates from Horreh were subjected to identification and grouping by cultural methods and the 16S rRNA sequencing. The new isolates were identified to be Lactobacillus (fermentum, plantarum, and brevis) Weissella cibaria, Enterococcus (faecium and faecalis), Leuconostoc (citreum and mesenteroides subsp...
May 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28508168/distinct-histone-modifications-modulate-defb1-expression-in-human-vaginal-keratinocytes-in-response-to-lactobacillus-spp
#14
Jaehyouk Lee, Ara Jang, Jin Wook Kim, Jun Hyun Han, Byung Hee Chun, Hye Su Jung, Che Ok Jeon, Soon Chul Myung
Vaginal commensal lactobacilli are considered to contribute significantly to the control of vaginal microbiota by competing with other microflora for adherence to the vaginal epithelium and by producing antimicrobial compounds. However, the molecular mechanisms of symbiotic prokaryotic-eukaryotic communication in the vaginal ecosystem remain poorly understood. Here, we showed that both DNA methylation and histone modifications were associated with expression of the DEFB1 gene, which encodes the antimicrobial peptide human β-defensin-1, in vaginal keratinocyte VK2/E6E7 cells...
May 15, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28497217/inhibition-of-clostridium-difficile-in-mice-using-a-mixture-of-potential-probiotic-strains-enterococcus-faecalis-nm815-e-faecalis-nm915-and-e-faecium-nm1015-novel-candidates-to-control-c-difficile-infection-cdi
#15
Nahla M Mansour, Walid F Elkhatib, Khaled M Aboshanab, May M A Bahr
This study is aimed at the isolation, identification, and characterization of potential probiotic strains capable of inhibiting Clostridium difficile in vitro and in vivo. Twenty isolates were isolated from infant fecal samples and screened against C. difficile using their cell-free supernatant. Only three isolates showed maximum inhibition from 56.05 to 60.60%, thus they were characterized for probiotic properties and safety. The results obtained approved their tolerance to the gastrointestinal tract conditions and safety profile...
May 11, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28493103/cellulose-derivatives-enhanced-stability-of-alginate-based-beads-loaded-with-lactobacillus-plantarum-lab12-against-low-ph-high-temperature-and-prolonged-storage
#16
Ismail M Fareez, Siong Meng Lim, Nurul Aida Ashyqin Zulkefli, Rakesh K Mishra, Kalavathy Ramasamy
The susceptibility of probiotics to low pH and high temperature has limited their use as nutraceuticals. In this study, enhanced protection of probiotics via microencapsulation was achieved. Lactobacillus plantarum LAB12 were immobilised within polymeric matrix comprised of alginate (Alg) with supplementation of cellulose derivatives (methylcellulose (MC), sodium carboxymethyl cellulose (NaCMC) or hydroxypropyl methylcellulose (HPMC)). L. plantarum LAB12 encapsulated in Alg-HPMC(1.0) and Alg-MC(1.0) elicited improved survivability (91%) in simulated gastric conditions and facilitated maximal release (∼100%) in simulated intestinal condition...
May 10, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28478573/tyrosinase-inhibitory-and-anti-oxidative-effects-of-lactic-acid-bacteria-isolated-from-dairy-cow-feces
#17
Keunho Ji, Youn Su Cho, Young Tae Kim
Overproduction and accumulation of melanin cause a number of skin diseases. The inhibitors of tyrosinase are important for the treatment of skin diseases associated with hyper-pigmentation after UV exposure and application in cosmetics for whitening and depigmentation. Reactive oxygen species (ROS) including hydrogen peroxide are generated by chemical substances and metabolic intermediates and cause various diseases including cancer and heart diseases. We have isolated four different lactic acid bacteria (LAB) strains from dairy cow feces and investigated the tyrosinase inhibition and anti-oxidative effects of culture filtrates prepared from the isolated bacteria, which are designated as EA3, EB2, PC2, and PD3...
May 6, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28455690/comparative-growth-behaviour-and-biofunctionality-of-lactic-acid-bacteria-during-fermentation-of-soy-milk-and-bovine-milk
#18
Subrota Hati, Nikita Patel, Surajit Mandal
The study reports the growth, acidification and proteolysis of eight selected lactic acid bacteria in skim and soy milk. Angiotensin-converting enzyme inhibition and antimicrobial profiles of skim and soy milk fermented by the lactic acid bacteria were also determined. Among eight lactic cultures (S. thermophilus MD2, L. helveticus V3, L. rhamnosus NS6, L. rhamnosus NS4, L. bulgaricus NCDC 09, L. acidophilus NCDC 15, L. acidophilus NCDC 298 and L. helveticus NCDC 292) studied, L. bulgaricus NCDC 09 and S. thermophilus MD2 decreased the pH of skim and soy milk in greater extent...
April 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28455689/aggregation-and-adhesion-activity-of-lactobacilli-isolated-from-fermented-products-in-vitro-and-in-vivo-a-potential-probiotic-strain
#19
S Grigoryan, I Bazukyan, A Trchounian
Approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. The strains Lactobacillus helveticus INRA-2010-H11, Lactobacillus rhamnosus INA-5.1, and Lactobacillus acidophilus JM-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). A high co-aggregation capacity (75.53%) for strains INRA-2010-H11 and JM-2012 was shown...
April 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28434154/protective-effects-of-cell-free-supernatant-and-live-lactic-acid-bacteria-isolated-from-thai-pigs-against-a-pandemic-strain-of-porcine-epidemic-diarrhea-virus
#20
Wandee Sirichokchatchawan, Gun Temeeyasen, Dachrit Nilubol, Nuvee Prapasarakul
Porcine epidemic diarrhea virus (PEDV) is a coronavirus which causes severe diarrhea and fatal dehydration in piglets. In general, probiotic supplements could enhance recovery and protect piglets against enteric pathogens. Seven local lactic acid bacteria (LAB), (Ent. faecium 79N and 40N, Lact. plantarum 22F, 25F and 31F, Ped. acidilactici 72N and Ped. pentosaceus 77F) from pig feces were well-characterized as high potential probiotics. Cell-free supernatants (CFS) and live LAB were evaluated for antiviral activities by co-incubation on Vero cells and challenged with a pandemic strain of PEDV isolated from pigs in Thailand...
April 22, 2017: Probiotics and Antimicrobial Proteins
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