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Probiotics and Antimicrobial Proteins

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https://www.readbyqxmd.com/read/28819794/in-vitro-selection-and-identification-of-potential-probiotics-isolated-from-the-gastrointestinal-tract-of-nile-tilapia-oreochromis-niloticus
#1
Rasha M Reda, Khaled M Selim, Hassanin M El-Sayed, M A El-Hady
Fish gut bacteria can be used as probiotics for aquaculture. The aim of this study is to screen and identify beneficial probiotic bacteria from the gut of Nile tilapia, Oreochromis niloticus. Nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, Aeromonas sobria, Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas putida, and Staphylococcus aureus. Further tests showed that such isolates can survive in the presence of high bile concentration (10%) and at different acidic pH values...
August 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28786012/the-effects-of-synbiotic-supplementation-on-pregnancy-outcomes-in-gestational-diabetes
#2
Maryam Karamali, Nabiollah Nasiri, Naemeh Taghavi Shavazi, Mehri Jamilian, Fereshteh Bahmani, Maryam Tajabadi-Ebrahimi, Zatollah Asemi
Synbiotics are known to exert multiple beneficial effects, including anti-inflammatory and antioxidative actions. This study was designed to evaluate the effects of synbiotic administration on biomarkers of inflammation, oxidative stress, and pregnancy outcomes among gestational diabetic (GDM) women. This randomized, double-blind, placebo-controlled clinical trial was carried out among 60 subjects with GDM who were not on oral hypoglycemic agents. Patients were randomly assigned to consume either one synbiotic capsule containing Lactobacillus acidophilus strain T16 (IBRC-M10785), L...
August 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28780720/evaluation-of-lactic-acid-bacteria-isolated-from-fermented-plant-products-for-antagonistic-activity-against-urinary-tract-pathogen-staphylococcus-saprophyticus
#3
Cheng-Chih Tsai, Tzu-Min Lai, Pei-Pei Lin, You-Miin Hsieh
Urinary tract infections (UTIs) are the most common infectious diseases in infants and the elderly; they are also the most common among nosocomial infections. The treatment of UTIs usually involves a short-term course of antibiotics. The purpose of this study was to identify the strains of lactic acid bacteria (LAB) that can inhibit the urinary tract pathogen Staphylococcus saprophyticus, as alternatives to antibiotics. In this study, we collected 370 LAB strains from fermented plant products and reference strains from the Bioresources Collection and Research Center (BCRC)...
August 5, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28770479/effect-of-milk-fermented-with-lactic-acid-bacteria-on-diarrheal-incidence-growth-performance-and-microbiological-and-blood-profiles-of-newborn-dairy-calves
#4
N C Maldonado, J Chiaraviglio, E Bru, L De Chazal, V Santos, M E F Nader-Macías
The effect of the administration of milk fermented with lactic acid bacteria to calves was evaluated. The strains included were: Lactobacillus murinus CRL1695, Lact. mucosae CRL1696, Lact. johnsonii CRL1693, and Lact. salivarius CRL1702, which were selected for their beneficial and functional properties and isolated from healthy calves in the northwestern region of Argentina. The trial was conducted on a dairy farm located in Tucumán (Holando-Argentino calves). A randomized controlled trial was performed in which 56 new-born animals were divided into two groups: the treated group (T) received the fermented milk for 60 days and the control group (C) only milk...
August 2, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28762193/probiotic-features-of-lactic-acid-bacteria-isolated-from-a-diverse-pool-of-traditional-greek-dairy-products-regarding-specific-strain-host-interactions
#5
Georgia Zoumpopoulou, Alexandra Tzouvanou, Eleni Mavrogonatou, Voula Alexandraki, Marina Georgalaki, Rania Anastasiou, Marina Papadelli, Eugenia Manolopoulou, Maria Kazou, Dimitris Kletsas, Konstantinos Papadimitriou, Effie Tsakalidou
The increased consumers' interest on the positive role of food in wellbeing and health underscores the need to determine new probiotic microorganisms. Triggered by the fact that artisanal food products can be a valuable source of novel probiotic strains, 106 lactic acid bacteria, all isolated from traditional Greek dairy products, namely Feta, Kasseri, Xynotyri, Graviera, Formaela, Galotyri, and Kefalotyri cheeses as well as yogurt and milk, were studied for probiotic properties. Based on their survival at pH 2...
July 31, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28756502/aciduric-strains-of-lactobacillus-reuteri-and-lactobacillus-rhamnosus-isolated-from-human-feces-have-strong-adhesion-and-aggregation-properties
#6
Kyle B Klopper, Shelly M Deane, Leon M T Dicks
Human feces were streaked onto MRS Agar adjusted to pH 2.5, 3.0, and 6.4, respectively, and medium supplemented with 1.0% (w/v) bile salts. Two aciduric strains, identified as Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 (based on 16S rDNA and recA sequences), were non-hemolytic and did not hydrolyze mucin. The surface of Lactobacillus reuteri HFI-LD5 cells has a weak negative charge, whereas Lactobacillus rhamnosus HFI-K2 has acidic and basic properties, and produces exopolysaccharides (EPS)...
July 29, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28752423/in-vitro-evaluation-of-the-probiotic-and-safety-properties-of-bacteriocinogenic-and-non-bacteriocinogenic-lactic-acid-bacteria-from-the-intestines-of-nile-tilapia-and-common-carp-for-their-use-as-probiotics-in-aquaculture
#7
Pierre Marie Kaktcham, Jules-Bocamdé Temgoua, François Ngoufack Zambou, Gloria Diaz-Ruiz, Carmen Wacher, María de Lourdes Pérez-Chabela
In this study, seven bacteriocinogenic and non-bacteriocinogenic LAB strains previously isolated from the intestines of Nile tilapia and common carp and that showed potent antibacterial activity against host-derived and non-host-derived fish pathogens were assayed for their probiotic and safety properties so as to select promising candidates for in vivo application as probiotic in aquaculture. All the strains were investigated for acid and bile tolerances, transit tolerance in simulated gastrointestinal conditions, for cell surface characteristics including hydrophobicity, co-aggregation and auto-aggregation, and for bile salt hydrolase activity...
July 27, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28744833/evaluation-of-in-vivo-probiotic-efficiency-of-bacillus-amyloliquefaciens-in-labeo-rohita-challenged-by-pathogenic-strain-of-aeromonas-hydrophila-mtcc-1739
#8
Ankita Nandi, Goutam Banerjee, Suhas Kumar Dan, Koushik Ghosh, Arun Kumar Ray
Diseases in aquatic organisms, including fish, are a major concern in aquaculture production. In this present investigation, we have evaluated the beneficial effects of dietary Bacillus amyloliquefaciens CCF7 (GenBank Acc. No. KP256501) supplementation in rohu (Labeo rohita) challenged by a pathogenic strain of Aeromonas hydrophila MTCC 1739. Four experimental diets were formulated: control diet (no probiotics) and three experimental diets (different concentrations of probiotic candidate B. amyloliquefaciens CCF7 at 10(5) (T1), 10(7) (T2), 10(9) (T3) CFU/g)...
July 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28744832/isolation-and-selection-of-potential-probiotic-bacteria-from-the-pig-gastrointestinal-tract
#9
Luca Lo Verso, Martin Lessard, Guylaine Talbot, Benoit Fernandez, Ismail Fliss
The present study aimed to isolate bacterial strains from the pig gastrointestinal tract that have antagonistic activity against potential pathogens and are able to produce antimicrobial compounds. That ability would be a first requirement for the strains' possible use as probiotics. Samples obtained from pig intestinal mucosa and contents were screened for the presence of antagonistic activity against pathogenic indicator strains of Escherichia coli, Salmonella, and Listeria by means of the double-layer technique...
July 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28741152/evaluation-of-probiotic-diversity-from-soybean-glycine-max-seeds-and-sprouts-using-illumina-based-sequencing-method
#10
Yali Huang, Miaomiao Zhang, Zujun Deng, Lixiang Cao
There is increasing interest in the use of plant probiotics as environmental-friendly and healthy biofertilizers. The study aimed at selecting for novel probiotic candidates of soybean (Glycine max). The bacteriome and mycobiome of soybean sprouts and seeds were analyzed by Illumina-based sequencing. Seeds contained more diverse bacteria than those in sprouts. The seeds contained similar fungal diversity with sprouts. Total 15 bacterial OTUs and 4 fungal OTUs were detected in seeds and sprouts simultaneously, suggesting that the sprouts contained bacterial and fungal taxa transmitted from seeds...
July 24, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28741151/probiotic-characteristics-of-lactobacillus-curvatus-dn317-a-strain-isolated-from-chicken-ceca
#11
Mohamed Zommiti, Nathalie Connil, Jeannette Ben Hamida, Mounir Ferchichi
The probiotic characteristics of Lactobacillus curvatus DN317, a strain isolated from chicken ceca, were evaluated. This strain was selected for study from the isolated Lactobacillus strains because it has specific anti-microbial activity against Campylobacter jejuni ATCC 33560, Camp. jejuni NCTC 11168, Listeria monocytogenes ATCC 7644, and Bacillus subtilis ATCC 8633. Lact. curvatus DN317 showed an auto-aggregation percentage of 72% and presented the highest co-aggregation with Lact. monocytogenes ATCC 7644 (68%) compared to B...
July 24, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28730403/functional-characterization-of-potential-probiotic-lactic-acid-bacteria-isolated-from-kalarei-and-development-of-probiotic-fermented-oat-flour
#12
Mahak Gupta, Bijender Kumar Bajaj
Considerable variations among probiotics with respect to their health benefitting attributes fuel the research on bioprospecting of proficient probiotic strains from various ecological niches especially the poorly unexplored ones. In the current study, kalarei, an indigenous cheese-like fermented milk product, and other dairy-based sources like curd and raw milk were used for isolation of lactic acid bacteria (LAB). Among 34 LAB isolates, 7 that could withstand simulated gastrointestinal (GI) conditions were characterized for functional probiotic attributes, viz...
July 20, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28712023/selection-of-potential-probiotic-lactobacillus-with-inhibitory-activity-against-salmonella-and-fecal-coliform-bacteria
#13
Mattika Abhisingha, Jureeporn Dumnil, Chetsadaporn Pitaksutheepong
Three hundred and sixty presumptive lactic acid bacteria (LAB) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-Salmonella activity. Fifty-eight isolates consisting of Lactobacillus reuteri (n = 32), Lactobacillus salivarius (n = 10), Lactobacillus mucosae (n = 8), Lactobacillus johnsonii (n = 5), and Lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substances, acid and bile tolerance, and cell adherence to Caco-2 cells...
July 15, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28712022/effects-of-dietary-lactobacillus-plantarum-on-growth-performance-digestive-enzymes-and-gut-morphology-of-litopenaeus-vannamei
#14
Xiaoting Zheng, Yafei Duan, Hongbiao Dong, Jiasong Zhang
A 15-day feeding trial was conducted to investigate the effect of dietary Lactobacillus plantarum on growth performance, digestive enzyme activities and gut morphology of juvenile Pacific white shrimp, Litopenaeus vannamei (initial body weight = 7.96 ± 0.59 g). Four microbound diets were formulated to contain fermentation supernatant (FS), live bacteria (LB), dead bacteria (DB), and cell-free extract (CE) of L. plantarum. Results indicated that final weight was significantly higher in FS, DB, and CE group in comparison to the control group (P < 0...
July 15, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28695539/elucidation-of-bacillus-subtilis-katmira-1933-potential-for-spore-production-in-submerged-fermentation-of-plant-raw-materials
#15
Tamar Khardziani, Eva Kachlishvili, Kakha Sokhadze, Vladimir Elisashvili, Richard Weeks, Michael L Chikindas, Vladimir Chistyakov
In this study, the effects of several key factors to increase spore production by Bacillus subtilis subsp. KATMIRA 1933 were evaluated in shake flask experiments. In a synthetic medium, glucose concentration played a crucial role in the expression of bacilli sporulation capacity. In particular, maximum spore yield (2.3 × 10(9) spores/mL) was achieved at low glucose concentration (2 g/L), and further gradual increase of the carbon source content in the medium caused a decrease in sporulation capacity. Substitution of glucose with several inexpensive lignocellulosic materials was found to be a reasonable way to achieve high cell density and sporulation...
July 10, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28677046/the-effects-of-synbiotic-supplementation-on-glucose-metabolism-and-lipid-profiles-in-patients-with-diabetes-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#16
REVIEW
Reza Tabrizi, Mahmood Moosazadeh, Kamran B Lankarani, Maryam Akbari, Seyed Taghi Heydari, Fariba Kolahdooz, Zatollah Asemi
Although several studies have evaluated the effect of synbiotic intake on metabolic profiles in patients with diabetes, findings are inconsistent. This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to summarize the evidence on the effect of synbiotic intake on metabolic profiles in patients with diabetes. The PubMed, EMBASE, Web of Science, and Cochrane Library databases were systematically searched. All RCTs published up to 12 November 2016 were included. Two review authors independently assessed study eligibility, extracted data, and evaluated risk of bias of included studies...
July 4, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28643226/capsular-polysaccharides-of-lactobacillus-spp-theoretical-and-practical-aspects-of-simple-visualization-methods
#17
Magdalena Oleksy, Elżbieta Klewicka
Lactobacillus strains can synthesize capsular polysaccharides (CPS), which are important substances in the dairy industry-they exhibit many important technological as well as health-promoting properties. Technological advancements have made it possible to detect bacterial capsules using costly and labor-intensive methods, such as serological reactions, molecular genetic techniques, and electron microscopy. Light microscopy, which is the method of interest in this paper, is one of the most widely accessible and cheapest techniques...
June 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28643225/using-illumina-based-sequence-analysis-to-guide-probiotic-candidate-selection-and-isolation
#18
Wenfeng Wang, Yi Li, Wangsen Qin, Changyi Sun, Hongming Tan, Lixiang Cao
Selection for probiotic candidates by in vivo experimental trials is time and labor consuming; more informed strategy is needed to select successful probiotic candidates. The aim of the study was to elucidate the microbial taxa transmitted from maize seeds to seedlings during the germination process of maize and their probiotic effects. The bacterial and fungal taxa in kernel germs and sprouts were analyzed by Illumina-based sequencing. The sprouts contained more diverse fungi than those in germs. The bacterial species (OTUs) declined with the germination from germs to the sprouts...
June 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28639176/combined-or-individual-effects-of-dietary-probiotic-pedicoccus-acidilactici-and-nucleotide-on-growth-performance-intestinal-microbiota-hemato-biochemical-parameters-and-innate-immune-response-in-goldfish-carassius-auratus
#19
Nooshin Mehdinejad, Mohammad Reza Imanpour, Valiollah Jafari
This study investigated the effect of dietary supplementation of probiotic Pedicoccus acidilactici and nucleotide (separately or combined) on growth performance, intestinal microbiota, hemato-immunological parameters, and immunity response in goldfish (Carassius auratus). Fish (average weight 5-6 g) were acclimatized and divided into eight experimental diets supplemented with P. acidilactici of different concentrations (0.1, 0.2, and 0.3% diet) and nucleotides (0 and 0.5% diet) for 6 months. Fish fed with experimental diets showed significant differences in terms of final weight, weight gain, feed conversion ratio, daily growth rate, and condition factor when compared to control diet (P < 0...
June 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28634812/semi-industrial-scale-production-of-a-new-yeast-with-probiotic-traits-cryptococcus-sp-ymhs-isolated-from-the-red-sea
#20
Ashraf F El-Baz, Hesham A El-Enshasy, Yousseria M Shetaia, Hoda Mahrous, Nor Zalina Othman, Ahmed E Yousef
A new yeast strain with promising probiotic traits was isolated from the Red Sea water samples. The isolate (YMHS) was subjected to genetic characterization and identified as Cryptococcus sp. Nucleotide sequence analysis of the rRNA gene internal transcribed spacer regions showed 95% sequence similarity between the isolate and Cryptococcus albidus. Cryptococcus sp. YMHS exhibited desirable characteristics of probiotic microorganisms; it has tolerance to low pH in simulated gastric juice, resistance to bile salts, hydrophobic characteristics, broad antimicrobial activity, and in vitro ability to degrade cholesterol...
June 20, 2017: Probiotics and Antimicrobial Proteins
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