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Probiotics and Antimicrobial Proteins

Haydee Eliza Romero-Luna, Humberto Hernández-Sánchez, Rosa María Ribas-Aparicio, Patricia Isidra Cauich-Sánchez, Gloria Dávila-Ortiz
Artisanal fermented beverages have been associated with beneficial effects for a long time. In Mexico, there are a wide variety of artisanal fermented beverages such as Tepache, where the fermentation is initiated by the addition of grains of a polysaccharide-containing biofilm matrix formed by a symbiotic association of bacteria and yeasts known as "Tibicos." These microorganisms can be responsible for the beneficial effect associated with fermented beverages inoculated with Tibicos. The probiotic potential of microorganisms has been widely studied, mainly in lactic acid bacteria, while despite the wide distribution of yeasts, these have not been so studied...
September 20, 2018: Probiotics and Antimicrobial Proteins
Awa Fanny Massounga Bora, Xiaodong Li, Yongming Zhu, Lingling Du
Freeze-dried banana powder represents an ideal source of nutrients and has not yet been used for probiotic incorporation. In this study, microencapsulation by freeze drying of probiotics Lactobacillus acidophilus and Lactobacillus casei was made using whey protein isolate (WPI), fructooligosaccharides (FOS), and their combination (WPI + FOS) at ratio (1:1). Higher encapsulation yield was found for (WPI + FOS) microspheres (98%). Further, microcapsules of (WPI + FOS) were used to produce a freeze-dried banana powder which was analyzed for bacterial viability under simulated gastrointestinal fluid (SGIF), stability during storage at 4 °C and 25 °C, and chemical and sensory properties...
September 19, 2018: Probiotics and Antimicrobial Proteins
Manisha Singh, Deepak Sharma, Rajni Chauhan, Gunjan Goel
The present study investigated skimmed milk and alginate-based encapsulation for protection of a probiotic strain, Lactobacillus gastricus BTM7 during storage and exposure to simulated gastrointestinal conditions. The investigations have revealed that coating with skimmed milk and alginate in a ratio of 1:1 resulted in highest encapsulation efficiency of 94% (p < 0.05) with approximately 1 log reduction in viable cell count and 90% release of encapsulated cells in 90 min. This formulation resulted in 5-fold higher survival of bacteria during storage at refrigeration for 21 days (p < 0...
September 19, 2018: Probiotics and Antimicrobial Proteins
Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti, Farzad Shidfar
Data on the effects of probiotics on adipokines such as omentin-1, nesfatin-1, and adropin are limited. The aim of this study was to evaluate the effects of probiotic yogurt along with a low-calorie diet (LCD) on serum omentin-1, adropin, and nesfatin-1 concentrations in obese and overweight individuals. Sixty obese or overweight individuals aged 20-50 years old were involved in this randomized double-blind placebo-controlled clinical trial. Participants were randomly allocated into two groups to consume either probiotic yogurt containing Lactobacillus acidophilus La5, Bifidobacterium BB12, and Lactobacillus casei DN001 (108  CFU/g each) (n = 30) or regular yogurt (n = 30) along with a LCD in both groups for 8 weeks...
September 19, 2018: Probiotics and Antimicrobial Proteins
Bao Le, Seung-Hwan Yang
Cholesterol-lowering activity is an important health benefit of lactic acid bacteria (LAB). This study aimed to screen LAB strains with cholesterol-lowering activities from salted fermented shrimp and evaluate probiotic characteristics and cholesterol-lowering potentials of these LAB isolates. Among 191 lactic acid strains isolated from traditional salted-fermented shrimp food, FB003 isolate showed the highest cholesterol-lowering activity and investigated as probiotics with cholesterol-lowering ability. Biochemical analysis and 16S rRNA sequencing revealed that this LAB isolate was Lactobacillus plantarum FB003...
September 18, 2018: Probiotics and Antimicrobial Proteins
Angélique Lewies, Lissinda H Du Plessis, Johannes F Wentzel
Antibiotic resistance is an imminent threat to the effective treatment of bacterial infections, and alternative antibiotic strategies are urgently required. The golden epoch of antibiotics is coming to an end, and the development of new therapeutic agents to combat bacterial infections should be prioritized. This article will review the potential of antimicrobial peptides (AMPs) to combat the threat of antimicrobial resistance. The modern-day antimicrobial resistance dilemma is briefly discussed followed by a review of the potential of AMPs to be used alone or in combination with current antibiotics in order to enhance antibacterial properties of antibiotics while also potentially combatting resistance...
September 18, 2018: Probiotics and Antimicrobial Proteins
Mahammed Zidour, Yanath Belguesmia, Benoit Cudennec, Thierry Grard, Christophe Flahaut, Sami Souissi, Djamel Drider
Here we show that Bacillus pumilus ICVB403 recently isolated from copepod eggs is able to produce, after 48-72 h of growth in Landy medium, extracellular inhibitory compounds, which are active against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, MRSA-S1, Staphylococcus epidermidis 11EMB, Staphylococcus warneri 27EMB, and Staphylococcus hominis 13EMB. Moreover, these extracellular inhibitory compound(s) were able to potentiate erythromycin against the aforementioned staphylococci...
September 18, 2018: Probiotics and Antimicrobial Proteins
Kavita Sharma, Sampan Attri, Gunjan Goel
The present study aimed to isolate and identify the potential probiotic lactic acid bacteria (LAB) from traditionally fermented wheat flour dough known as "babroo." A total of 26 lactic isolates were obtained from 50 different samples collected from different locations of the Himalayan province (Himachal Pradesh). To ascertain non-pathogenic nature, the isolates were screened by hemolytic activity leading to 20 non-hemolytic strains which were subjected to tolerance to simulated gastrointestinal conditions...
September 15, 2018: Probiotics and Antimicrobial Proteins
Navid Mazruei Arani, Zahra Emam-Djomeh, Hamid Tavakolipour, Reza Sharafati-Chaleshtori, Alireza Soleimani, Zatollah Asemi
To the best of our knowledge, this study is the first evaluating the effects of probiotic honey intake on glycemic control, lipid profiles, biomarkers of inflammation, and oxidative stress in patients with diabetic nephropathy (DN). This investigation was conducted to evaluate the effects of probiotic honey intake on metabolic status in patients with DN. This randomized, double-blind, controlled clinical trial was performed among 60 patients with DN. Patients were randomly allocated into two groups to receive either 25 g/day probiotic honey containing a viable and heat-resistant probiotic Bacillus coagulans T11 (IBRC-M10791) (108  CFU/g) or 25 g/day control honey (n = 30 each group) for 12 weeks...
September 14, 2018: Probiotics and Antimicrobial Proteins
A S Vasilchenko, V A Gritsenko, D B Kosyan, E A Rogozhin
The aim of this study was to characterize the structure and mode of action of antimicrobials derived from a commercial preparation of alfa-interferon. By combination of semi-preparative/analytical reversed-phase high-performance liquid chromatography, we isolated and purified a novel active substance based on carbohydrate with a complex of amino acids, which determines antimicrobial property of commercial preparation of interferon. A size-exclusion chromatography was performed and LC/ESI-MS revealed molecular masses of active substance were in the range of 180-249 Da...
September 13, 2018: Probiotics and Antimicrobial Proteins
Sultan Arslan-Tontul, Mustafa Erbas, Ahmet Gorgulu
To date, the probiotic product development studies have mostly focused on dairy-based foods. However, endowing bakery products with probiotic properties not only provides a variety in food selection but would also potentially improve public health when the consumption rates are taken into consideration. This study aimed to incorporate single- and double-layered microcapsules containing Saccharomyces boulardii, Lactobacillus acidophilus, and Bifidobacterium bifidum, produced by spray drying and chilling, in cake production...
September 13, 2018: Probiotics and Antimicrobial Proteins
Mehdi Soltani, Gholamreza Badzohreh, Saed Mirzargar, Mehrdad Farhangi, Pezhman Hosseini Shekarabi, Alan Lymbery
Growth behavior and production of short-chain fatty acid (SCFA) of two probiotics, Pediococcus acidilactici and Lactococcus lactis, each at 107  cfu/g (P1, L1) and 1010  cfu/g (P2, L2) at different concentrations of fructooligosaccharide (FOS) [0.5% (F1), 1% (F2), and 2% (F3)] were assessed in vitro. The time to reach the maximum growth of the probiotics in all 12 treatments was between 8 to 10 h, with the highest and the lowest growth rates obtained in F1L1P1 (0.34 ± 0.02 OD) and F3L1P1 (0.31 ± 0...
September 6, 2018: Probiotics and Antimicrobial Proteins
Ewa Laskowska, Łukasz Sebastian Jarosz, Zbigniew Grądzki
The aim of the study was to determine the effect of EM Bokashi® on the phagocytic activity of monocytes and granulocytes, oxidative burst, SWC3, and CD11b + CD18+ expression on monocytes and granulocytes, and the serum concentration of cytokine and lysozyme in pig. 60 Sixty female piglets were divided into two groups: I - control and II - experimental. For the experimental group, a probiotic in the form of the preparation EM Bokashi® was added to the basal feed. Flow cytometry was used to determine selected non-specific immune response parameters, intracellular production of hydrogen peroxide by peripheral granulocytes and monocytes, and surface particles in peripheral blood...
September 5, 2018: Probiotics and Antimicrobial Proteins
Reda Tarabees, Khaled M Gafar, Mohamed S El-Sayed, Awad A Shehata, Marwa Ahmed
The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78. For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)-G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E...
September 4, 2018: Probiotics and Antimicrobial Proteins
B Houyvet, A Leduc, V Cornet, J Pontin, L Benoist, B Bernay, J Henry, E Vetois, C Zatylny-Gaudin
K4 is a de novo peptide with antibacterial activity on human pathogens. It has a short sequence (14 amino acids), with a cationic N-terminal moiety and an amphipathic ɑ-helix structure. The present paper demonstrates its activity on Vibrio bacteria in a marine environment. It was found non-toxic on marine organisms including Artemia salina, Dicentrarchus labrax, and Magallana gigas at different developmental stages, but influenced the growth of unicellular organisms like microalgae, depending on the algal strain and on K4 concentration...
August 24, 2018: Probiotics and Antimicrobial Proteins
E I Ermolenko, Y A Desheva, A A Kolobov, M P Kotyleva, I A Sychev, A N Suvorov
In spite of scientific evidence demonstrating the antiviral activity of lactic-acids bacteria, little is known about the mechanism of their action. Previously, several bacteriocins isolated from lactic acid bacteria (LAB) and some other microorganisms were reported as having antiviral activity in vitro. In the present study, chemically synthetized enterocin B (EntB) and the strain E. faecium L3, known as the producer of this peptide, were tested for activity against influenza viruses. The inhibition of cytopathic effect of А/Perth/16/2009(H3N2) and A/South Africa/3626/2013(H1N1) pdm influenza viruses in MDCK cells by chemically synthetized EntB was revealed...
August 24, 2018: Probiotics and Antimicrobial Proteins
Dorota Zielińska, Ewa Długosz, Anna Zawistowska-Deniziak
In the current study, the probiotic activity of ten Lactobacillus (Lb.) strains, previously isolated from the traditional Polish fermented vegetable, was characterized. Strains were assessed for adhesion to human intestinal epithelial Caco-2 cells and regulation of selected cytokine production (IL-1β, IL-6, IL-10, IL-23, and TNF-α) by THP-1 macrophages. The effect of tested strains on Caco-2 cell apoptosis was also investigated using a caspase-3 assay. Adhesion capacity was strain-dependent (1.29-8.24% of initial population)...
August 23, 2018: Probiotics and Antimicrobial Proteins
Farhana Hoque, T Jawahar Abraham, T S Nagesh, Dibyendu Kamilya
Use of probiotics as the biocontrol agent for disease prevention in aquaculture is gaining importance as an alternative to the indiscriminate use of antibiotics and other chemotherapeutics. In view of this trend, the probiotic properties of a potent antagonistic bacterium, Pseudomonas aeruginosa FARP72 , was characterized in terms of safety, antagonistic activities, in vitro immunomodulation, and in vivo disease resistance. Immunomodulatory activity was ascertained by measuring the production of intracellular superoxide anion, nitric oxide, total leukocyte peroxidase content, and the leukocyte proliferation in head kidney leukocytes...
August 15, 2018: Probiotics and Antimicrobial Proteins
Javad Sahandi, Hojatollah Jafaryan, Mehdi Soltani, Pouneh Ebrahimi
This study aims to evaluate the effects of Bifidobacterium animalis PTCC-1631 and Bifidobacterium lactis PTCC-1736 on growth, nutrient digestibility, carcass composition, and intestinal microbiota of Oncorhynchus mykiss fry, which had initial body weights of 0.58 ± 0.19 g. Four iso-nitrogenous diets with probiotics, namely T1 (1 × 107  CFU g-1 ), T2 (2 × 107  CFU g-1 ), T3 (3 × 107  CFU g-1 ), and control without probiotics were obtained. Total 480 fish were randomly divided in four treatments (three experimental treatments and a control), each with 4 replicates including 16 tanks each filled with 15 L-1 of water (water exchange rate of 2 L min-1 and aerated with air stone) with a density of 2 fry L-1 ...
August 14, 2018: Probiotics and Antimicrobial Proteins
Nasrollah Ahmadifard, Vahid Rezaei Aminlooi, Amir Tukmechi, Naser Agh
The present study investigated the effect of enriched Artemia with Bacillus subtilis on growth performance, reproductive factors, proximate composition, intestinal microflora, and resistance to Aeromonas hydrophila of ornamental fish, Poecilia latipinna. Using a completely randomized design, the experiment included three groups. The first group was fed with commercial food without any probiotic. The second group was fed with unenriched Artemia, and the last group consumed long-time enriched Artemia with Bacillus subtilis...
August 10, 2018: Probiotics and Antimicrobial Proteins
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