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Probiotics and Antimicrobial Proteins

Nima Montazeri-Najafabady, Younes Ghasemi, Mohammad Hossein Dabbaghmanesh, Pedram Talezadeh, Farhad Koohpeyma, Ahmad Gholami
Osteoporosis is a major health problem that occurs as a result of an imbalance between bone formation and bone resorption. Different approaches have been established for treating osteoporosis. Recently, because of their health benefits and also low adverse reaction, probiotics have been receiving considerable attention. In this study, we compared the effectiveness of five probiotic strains, Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Bifidobacterium longum, and Bacillus coagulans, in protecting rats from ovariectomized (OVX)-induced bone loss...
July 17, 2018: Probiotics and Antimicrobial Proteins
Ismail M Fareez, Siong Meng Lim, Kalavathy Ramasamy
Lactic acid bacteria (LAB) with probiotic properties are useful options for prophylactic and therapeutic applications against gastrointestinal diseases. The safety of probiotics should, however, be verified before incorporation into food or drinks. The present study had encapsulated Lactobacillus plantarum LAB12 within microcapsules that could withstand extremely high temperature (up to 100 °C) during pelletisation. The microencapsulated LAB12 were then tested for their acute (single dosing) and sub-chronic (a 90-day feeding) toxicity...
July 12, 2018: Probiotics and Antimicrobial Proteins
Ke Wang, Xiaohong Yu, Yi Li, Yun Guo, Lin Ge, Fangfang Pu, Xinying Ma, Wenjing Cui, Francesco Marrota, Fang He, Ming Li
Bifidobacterium bifidum TMC3115 strain (TMC3115) was orally administrated to 47 subjects with mild hyperglycaemia and dyslipidaemia aged 45 to 75 years for 3 weeks. Blood samples were collected before and after intervention for profiling plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol and triglyceride concentrations, as well as fasting blood glucose. Before and 3 and 4 weeks after intervention, the faecal samples were collected to analyse faecal microbiota using the sequencing of 16S rRNA genes with a next-generation sequencer...
July 5, 2018: Probiotics and Antimicrobial Proteins
Maxim S Makarenko, Vladimir A Chistyakov, Alexander V Usatov, Maria S Mazanko, Evgeniya V Prazdnova, Anzhelika B Bren, Ivan F Gorlov, Zoya B Komarova, Michael L Chikindas
In the current study, we performed in vivo investigation of probiotic intake influence on nuclear and mitochondrial DNA damage of hens, using quantitative PCR techniques. The probiotic supplementation to the diet of Hisex Brown hens had no significant effect on the rate of telomere shortening. After prolonged probiotic intake (225 and 445 days), the 18-21% decrease in the mtDNA lesions was detected. Since avian mitochondrial DNA damage investigations are rare, the current study of the probiotic-enriched diet's impact on the damage of the hen mitochondrial DNA is novel and highly important...
July 4, 2018: Probiotics and Antimicrobial Proteins
Elzaan Booysen, Martin Bezuidenhout, Anton Du Preez van Staden, Dimiter Dimitrov, Shelly M Deane, Leon M T Dicks
Vancomycin is often used to treat infections caused by β-lactam-resistant bacteria. However, methicillin-resistant strains of Staphylococcus aureus (MRSA) acquired resistance to vancomycin, rendering it less effective in the treatment of serious infections. In the search for novel antibiotics, alternative delivery mechanisms have also been explored. In this study, we report on the encapsulation of vancomycin in PLGA [poly(DL-lactide-co-glycolide)] nanoparticles by electrospraying. The nanoparticles were on average 247 nm in size with small bead formations on the surface...
June 30, 2018: Probiotics and Antimicrobial Proteins
Sergio Alonso, M Carmen Castro, Margarita Berdasco, Inés García de la Banda, Xabier Moreno-Ventas, Alma Hernández de Rojas
With the increase of antimicrobial resistances due to the widespread use of antibiotics, the search of new probiotics to control aquaculture diseases has a growing public interest. The aim of this study was to isolate bacteria with antimicrobial effect from the gut of marine healthy fishes and select lactic acid bacteria (LAB) as potential probiotics, being strains considered as generally regarded as safe (GRAS) by the European Food Safety Agency (EFSA). Of a total of 45 Gram-positive strains with antimicrobial activity found in a screening of the gut microbiota of 13 marine fishes, nine were identified as LAB by 16S rRNA gene sequencing...
June 29, 2018: Probiotics and Antimicrobial Proteins
Rohini Sabu, R Aswani, K S Nidheesh, J G Ray, A Remakanthan, E K Radhakrishnan
Plant probiotic mechanisms of endophytic microorganisms are highly remarkable as it play key role in growth and health of plants. Even though Burkholderia spp. have been studied for their role in plant growth and disease management, report on their field performance is very limited. Hence, the objective of the study was to investigate the plant probiotic performance of selected Burkholderia spp. on Capsicum frutescens. The results of the study showed bacterial influence on growth of C. frutescens with remarkable induction of early flowering and fruiting...
June 25, 2018: Probiotics and Antimicrobial Proteins
Biswaranjan Pradhan, Dipanjan Guha, Aman Kumar Naik, Arka Banerjee, Subodh Tambat, Saurabh Chawla, Shantibhusan Senapati, Palok Aich
Gut microbiota play important role in maintaining health. Probiotics are believed to augment it further. We aimed at comparing effects of probiotics, Lactobacillus acidophilus (LA) and Bacillus clausii (BC) (a) on the gut microbiota abundance and diversity and (b) their contributions to control intestinal dysbiosis and inflammation in Th1- and Th2-biased mice following Salmonella infection. We report how could gut microbiota and the differential immune bias (Th1 or Th2) of the host regulate host responses when challenged with Salmonella typhimurium in the presence and absence of either of the probiotics...
June 16, 2018: Probiotics and Antimicrobial Proteins
Liqing Huang, Liping Luo, Yaru Zhang, Zhong Wang, Zhaofei Xia
The effects of Enterococcus faecium on growth, intestinal barrier function, and immune response in Escherichia coli O78-challenged broiler chickens were investigated. Three hundred eight 1-day-old Ross male chickens were randomly assigned into three treatment groups: negative control (C), E. coli O78-infected positive (EP), and E. coli O78-infected with 200 mg/kg E. faecium dietary supplementation (EF). E. faecium significantly increased the body weight on day 10 (P < 0.05) and day 15. Furthermore, these birds had a greater average daily gain compared with the other groups during days 1-10 (P < 0...
June 12, 2018: Probiotics and Antimicrobial Proteins
Ahmad Oryan, Esmat Alemzadeh, Mohammad Hadi Eskandari
Kefir is a natural probiotic compound with a long history of health benefits which can improve wound healing. This study investigated the regeneration potential of kefir in vitro scratch assay and in vivo burn wound in rat model. Cytotoxicity of different concentrations of kefir was evaluated by colorimetric methylthiazoltetrazolium assay. A scratch wound experiment was performed to investigate the ability of kefir in reducing the gap of wounds in a dose-dependent manner, in vitro. The standardized kefir was incorporated into silver sulfadiazine (SSD) and applied on burn wounds in vivo, and was compared with the SSD and negative control groups after 7, 14, and 28 days of treatment...
June 8, 2018: Probiotics and Antimicrobial Proteins
Ziyanda C Dlamini, Rashwahla L S Langa, Olayinka A Aiyegoro, Anthony I Okoh
The study evaluated the safety and colonisation properties of four lactic acid bacteria (LAB), by determining their cell hydrophobicity and aggregation abilities. In addition, the presence of virulence and resistance genes was assayed in these probiotic candidates. Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBRC13956 were tested for cell surface hydrophobicity abilities against xylene, chloroform and ethyl acetate. The isolates were also tested for auto-aggregation and co-aggregation abilities; the optical densities of cell growth were measured after 1, 2, 3 and 4 h of experimental set-up...
June 7, 2018: Probiotics and Antimicrobial Proteins
J E Aguilar-Toalá, H Astiazarán-García, M C Estrada-Montoya, H S Garcia, B Vallejo-Cordoba, A F González-Córdova, A Hernández-Mendoza
It has been recognized that lactic acid bacteria exhibit antioxidant properties, which have been mainly endorsed to the intact viable bacteria. However, recent studies have shown that intracellular content (IC) may also be good sources of antioxidative metabolites, which may potentially contribute to oxidative homeostasis in vivo. Hence, the modulatory effect of the intracellular content of Lactobacillus casei CRL 431 (IC431) on aflatoxin B1 (AFB1 )-induced oxidative stress in rats was evaluated on the basis of its influence on hepatic lipid peroxidation (LPO), antioxidant status-antioxidant capacity (TAC), catalase (CAT), and glutathione peroxidase (GPx) activities; and on the oxidative stress index (OSi)...
June 3, 2018: Probiotics and Antimicrobial Proteins
Shagun Choudhary, Manisha Singh, Deepak Sharma, Sampan Attri, Kavita Sharma, Gunjan Goel
The present study aimed to screen the indigenous probiotic cultures for their effect on total phenolic contents (TPC) and associated antioxidant activities in synbiotic fermented soymilk during storage. Among 16 cultures, subtractive screening was conducted based on different tests such as acidification rate and proliferation of lactic acid bacteria (LAB) on supplementation of inulin (0-20 mM) and fructooligosaccharide (0-0.45 mM). Lactobacillus paracasei CD4 was selected as potential strain after principal component analysis (PCA) of different strains with prebiotic substrates at different concentrations...
June 2, 2018: Probiotics and Antimicrobial Proteins
Yafei Duan, Jiasong Zhang, Jianhua Huang, Shigui Jiang
The present study investigated the effects of the dietary probiotic Clostridium butyricum (CB) on the growth, intestine digestive enzyme activity, antioxidant capacity and resistance to nitrite stress, and body composition of Penaeus monodon. For 56 days, shrimps were fed diets containing different levels of C. butyricum (1 × 109  CFU g-1 ), 0% (control), 0.5% (CB1), 1.0% (CB2), and 2.0% (CB3), as treatment groups, followed by an acute nitrite stress test for 48 h. The results indicated that dietary supplementation of C...
June 1, 2018: Probiotics and Antimicrobial Proteins
Jyoti Choudhary, R C Dubey, Gyanendra Sengar, Shrivardhan Dheeman
Lactic acid bacteria isolated from indigenous milk of different animals were investigated for their efficacy, safety, and probiotic potential. The most potential isolate MMP4 was screened from mare's milk, which was further identified as Lactobacillus pentosus by using 16S rRNA gene sequencing and phylogeny. The probiotic potential of strain MMP4 was assessed by its ability to survive under acidic environment and in presence of bile salts along with the ability to inhibit food-borne as well as clinical pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Salmonella typhi...
May 30, 2018: Probiotics and Antimicrobial Proteins
Kyle B Klopper, Elanna Bester, Shelly M Deane, Gideon M Wolfaardt, Leon M T Dicks
In this study, we report on the formation and resilience of Lactobacillus reuteri HFI-LD5 and Lactobacillus rhamnosus HFI-K2 biofilms cultivated in a CO2 evolution measurement system (CEMS) and exposed to biologically relevant, fasting-state gastrointestinal fluids under continuous flow conditions. For comparative purposes, planktonic and sessile populations of L. reuteri HFI-LD5 and L. rhamnosus HFI-K2 were each exposed to fasting-state gastric fluid (FSGF, pH 2.0) for 2 h, fasting-state intestinal fluid (FSIF, pH 7...
May 21, 2018: Probiotics and Antimicrobial Proteins
Radha Yadav, Suhail Hakeem Khan, Sanusi Bello Mada, Sunita Meena, Rajeev Kapila, Suman Kapila
There is a growing and alarming prevalence that increased serum cholesterol is closely related to increased cardiovascular disease risk. Probiotic consumption could be a safe and natural strategy to combat. Therefore, we sought to examine the cholesterol-lowering potential of co-supplementation of probiotic bacteria Lactobacillus fermentum MTCC: 5898-fermented buffalo milk (2.5% fat) in rats fed cholesterol-enriched diet. Male Wistar rats were divided into three groups on the basis of feed, viz. group 1, fed standard diet (SD); group 2, fed cholesterol-enriched diet (CED); and group 3, fed cholesterol-enriched diet along with L...
May 13, 2018: Probiotics and Antimicrobial Proteins
J J Ahire, N U Mokashe, B L Chaudhari
In this study, we have investigated the effect of probiotic Lactobacillus helveticus CD6 supplemented into simple laboratory fish feed (LFF) and complex, multi-ingredient market fish feed (MFF) on growth performance, antioxidant levels, and essential trace element absorption in goldfish (Carassius auratus). Twenty-four healthy goldfish (average weight 3-4 g) were acclimatized and divided into four experimental diets supplemented with 3 × 107  CFU/g of probiotic (LFF + Pro, MFF + Pro) and without probiotic (LFF, MFF) for 14 weeks...
May 10, 2018: Probiotics and Antimicrobial Proteins
Ourdia Kareb, Mohammed Aïder
The purpose of this review is to highlight the importance of whey as a source of new-generation functional ingredients. Particular interest is given to probiotic growth in the presence of whey derivatives such as lactulose, a lactose derivative, which is a highly sought-after prebiotic in functional feeding. The role of sugar/nitrogen interactions in the formation of Maillard products is also highlighted. These compounds are known for their antioxidant power. The role of bioactive peptides from whey is also discussed in this study...
May 7, 2018: Probiotics and Antimicrobial Proteins
Jian Liu, Deping Han, Yawei Shi
Thaumatin-like proteins (TLPs) are the products of a large, highly complex gene family involved in host defense. TLPs also belong to the pathogenesis-related family 5 (PR-5) of plant defense proteins. Most TLPs exhibit potential antifungal activities, and their accumulation in the plant is related to many physiological processes. In this study, a gene encoding TLP named permatin with an open reading frame of 678 bp encoding a protein of 225 amino acids with a calculated molecular mass of 23.5 kDa was cloned from naked oat leaves...
May 2, 2018: Probiotics and Antimicrobial Proteins
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