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Drug Testing and Analysis

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https://www.readbyqxmd.com/read/29319234/comparative-pharmacokinetics-of-trandolapril-its-active-metabolite-and-verapamil-in-human-plasma-of-egyptian-population-using-hplc-ms-ms
#1
Ragaa Magdy, Ahmed H El-Khatib, Ahmed Hemdan, Omar Abd Elaziz, Maha Farouk, Michael W Linscheid
Trandolapril and verapamil are commonly used antihypertensive drugs. However, there is a lack of available data on the change of their pharmacokinetics in patients with liver or kidney impairment and hence the need for dose adjustment. In the presented work, a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the monitoring of trandolapril, its active metabolite trandolaprilat, and verapamil in human plasma of patients with renal impairment and/or liver insufficiency...
January 10, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29314761/keratinous-matrices-for-the-assessment-of-drugs-of-abuse-consumption-a-correlation-study-between-hair-and-nails
#2
Delphine Cappelle, Steven De Keukeleire, Hugo Neels, Frederic Been, Mireille De Doncker, Geert Dom, Cleo L Crunelle, Adrian Covaci, Alexander L N van Nuijs
Keratinous matrices - hair and nails - accumulate substances over time and allow retrospective investigation of past consumption. Analysis of these matrices can provide information complementary to blood and urine analysis or can be used as standalone. So far, research has primarily focused on the detection of substances in hair, while studies in nails are scarce. In this study, we assessed concentrations of drugs of abuse and their metabolites in hair, finger- and toenails collected from the same individuals to evaluate differences and correlations between matrices...
January 5, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29314710/different-in-vitro-and-in-vivo-tools-for-elucidating-the-human-metabolism-of-alpha-cathinone-derived-drugs-of-abuse
#3
Sascha K Manier, Lilian H J Richter, Jan Schäper, Hans H Maurer, Markus R Meyer
In vitro and in vivo experiments are widely used for studying the metabolism of new psychoactive substances (NPS). The availability of such data is required for toxicological risk assessments and development of urine screening approaches. This study investigated the in vitro metabolism of the five pyrrolidinophenone-derived NPS alpha-pyrrolidinobutyrophenone (alpha-PBP), alpha-pyrrolidinopentiothiophenone (alpha-PVT), alpha-pyrrolidinohexanophenone (alpha-PHP), alpha-pyrrolidinoenanthophenone (alpha-PEP, PV8), and alpha-pyrrolidinooctanophenone (alpha-POP, PV9)...
January 4, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29314750/phase-i-metabolism-of-the-recently-emerged-synthetic-cannabinoid-cumyl-pegaclone-and-detection-in-human-urine-samples
#4
Lukas Mogler, Maurice Wilde, Laura M Huppertz, Georg Weinfurtner, Florian Franz, Volker Auwärter
Indole, indazole or azaindole based synthetic cannabinoids (SCs), bearing a cumyl substituent are a widespread, recreationally used subgroup of new psychoactive substances (NPS). The latest cumyl-derivative, CUMYL-PEGACLONE, emerged in December 2016 on the German drug market. The substance features a novel γ-carboline core structure, which is most likely synthesized to bypass generic legislative approaches to control SCs by prohibiting distinct core structures. Using liquid chromatography tandem mass spectrometry and liquid chromatography high resolution mass spectrometry techniques, the main in vivo phase I metabolites of this new substance were detected...
January 3, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29314653/identification-of-oxymorphone-as-decomposition-product-of-the-permitted-drug-methylnaltrexone
#5
LETTER
T Sobolevsky, Y Kucherova, B Ahrens
No abstract text is available yet for this article.
January 3, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29287305/development-of-an-immunochromatographic-strip-incorporating-anti-mitragynine-monoclonal-antibody-conjugated-to-colloidal-gold-for-kratom-alkaloids-detection
#6
Supattra Limsuwanchote, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto, Niwat Keawpradub, Juraithip Wungsintaweekul
A lateral flow-based immunochromatographic strip was developed for the rapid detection of mitragynine (MG), a dominant alkaloid found in the leaves of kratom. Monoclonal antibody (mAb) against MG (anti-MG mAb) was conjugated to colloidal gold and used as a recognition probe. MG-ovalbumin conjugate (MG-OVA) and goat anti-mouse IgG were immobilized on the strip to produce a test zone and control zone, respectively. Based on the principle of a competitive assay, MG in a test sample competed with MG-OVA resident in the test zone to bind with colloidal gold-anti-MG mAb, resulting in an inverse relation of color intensity at the test zone and MG amount...
December 29, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29278456/growth-hormone-isoform-differential-mass-spectrometry-for-doping-control-purposes
#7
Cristian G Arsene, Dirk Schulze, Anita Röthke, Mario Thevis, André Henrion
Mass spectrometry (MS) allows for monitoring growth-hormone (GH) isoform compositions at high specificity. It is demonstrated, that this capability can be used to reliably detect alterations as elicited by (putative) doping with 22 kDa-GH. Sample treatment consists in enzymatic protein cleavage, followed by two-step liquid chromatography clean-up, prior to analysis by MS. The protocol does not depend on antibodies for analyte extraction, at any stage. Therefore, MS opens an opportunity to independent confirmation, if combined with the antibody-based isoform differential test presently used in practice...
December 26, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29278450/effect-of-glucocorticoid-administration-on-the-steroid-profile
#8
Sergi Coll, Xavier Matabosch, Lorena Garrostas, Clara Perez-Maña, Rosa Ventura
The steroid profile (SP) is a powerful tool to detect the misuse of endogenous anabolic androgenic steroids in sports, and it is included in the athlete biological passport (ABP). Glucocorticoids (GCs), which are widely prescribed in sports and are only prohibited in competition by systemic routes, inhibit the hypothalamic-pituitary-adrenal axis. Since the metabolites monitored in the SP have a partial adrenal origin, their excretion in urine might be altered by GCs consumption. The aim of the present work was to investigate if GCs administered by either systemic or local routes could influence the SP parameters...
December 26, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29232492/use-of-split-free-nlc-ms-hrms-interface-to-improve-the-detection-of-%C3%AE-cobratoxin-in-equine-plasma-for-doping-control
#9
Ludovic Bailly-Chouriberry, Patrice Garcia, Florence Cormant, Benoit Loup, Marie-Agnès Popot, Yves Bonnaire
Cobra (Naja naja kaouthia) venom contains a toxin called α-cobratoxin (α-Cbtx) containing 71 amino acids (MW 7821 Da) with a reported analgesic power greater than morphine. In 2013, the first analytical method for the detection of α-Cbtx in equine plasma was developed by Bailly-Chouriberry et al. allowing the confirmation of the presence of α-Cbtx at low concentrations (1-5 ng/mL or 130-640 fmol/mL) in plasma samples. In order to increase the method sensitivity and therefore to improve the detection of α-Cbtx in post-administration plasma samples, a nano-liquid chromatography - mass spectrometry/high resolution mass spectrometry (nLC-MS/HRMS) method was developed...
December 12, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29214743/gc-ms-ms-detects-potential-pregabalin-abuse-in-susceptible-subjects-hair
#10
Federica Ianni, Kyriaki Aroni, Alessio Gili, Roccaldo Sardella, Mauro Bacci, Massimo Lancia, Benedetto Natalini, Cristiana Gambelunghe
Pregabalin, a GABA analogue, binds to the alpha 2 delta subunit of voltage-dependent calcium channels. It is recognized as efficacious in pathologies such as epilepsy, neuropathic pain and anxiety disorders. Since pregabalin prescriptions have increased worldwide, reports of its abuse have been accumulating, mainly in patients with opioid abuse disorders. The present study investigated potential pregabalin abuse by means of hair analysis, a matrix that provides valuable retrospective information. Half of the pool of 280 susceptible patients had been occasional drug users and were being monitored for driving licence renewals...
December 7, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29205946/surveillance-of-drug-abuse-in-hong-kong-by-hair-analysis-using-lc-ms-ms
#11
K Wing Leung, Zack C F Wong, Janet Y M Ho, Ada W S Yip, Jerry K H Cheung, Karen K L Ho, Ran Duan, Karl W K Tsim
The aim of this study is to reveal the habits of drug abusers in hair samples from drug rehabilitation units in Hong Kong. With the application of LC-MS/MS technology, a total of 1,771 hair samples were analyzed during the period (Jan 2012 to Mar 2016) of hair testing service provided to fourteen drug rehabilitation units including non-governmental organizations (NGOs), rehabilitation centers and medical clinics. Hair samples were analyzed for abused drugs and their metabolites simultaneously, including ketamine, norketamine, cocaine, benzoylecgonine, cocaethylene, norcocaine, codeine, MDMA, MDA, MDEA, amphetamine, methamphetamine, morphine, 6-acetylmorphine, phencyclidine and methadone...
December 4, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29193906/detection-of-sotatercept-ace-011-in-human-serum-by-sar-page-and-western-single-blotting
#12
Christian Reichel, Letizia Farmer, Günter Gmeiner, Katja Walpurgis, Mario Thevis
A method for the detection of Sotatercept (ACE-011, ACVR2A-Fc) in human serum is presented. The method is a modification of a recently published protocol for Luspatercept (ACE-536, ACVR2B-Fc), another erythropoiesis stimulating fusion protein. Out of 27 tested antibodies against either the extracellular domain of ACVR2A or the full length protein, only 4 antibodies bound strongly enough to Sotatercept for usage with immunoprecipitation followed by SAR-PAGE and Western single blotting. The adapted protocol allows the detection of 0...
November 28, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29172026/effect-of-non-prohibited-drugs-on-the-phase-ii-metabolic-profile-of-morphine-an-in-vitro-investigation-for-doping-control-purposes
#13
Gabriella Ambrosio, Xavier de la Torre, Monica Mazzarino, Maria K Parr, Francesco Botrè
The potential consequences of drug-drug interaction on the strategies adopted by the anti-doping laboratories to report an adverse analytical finding for morphine were investigated. We have evaluated in vitro the effects of fourteen drugs on the principal metabolic pathways of morphine. The selected drugs are among those most commonly used by the athletes, none of them presently included in the World Anti-Doping Agency Prohibited List. The non-prohibited drugs included four antifungals (fluconazole, itraconazole, ketoconazole and miconazole), six benzodiazepines (alprazolam, bromazepam, clonazepam, lorazepam, lormetazepam and triazolam), and four non-steroidal anti-inflammatory drugs (diclofenac, ibuprofen, ketoprofen and nimesulide)...
November 24, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29166551/evaluation-of-markers-out-of-the-steroid-profile-for-the-screening-of-testosterone-misuse-part-ii-intramuscular-administration
#14
Aristotelis Kotronoulas, Àlex Gomez-Gómez, Andreu Fabregat, Jordi Segura, Sheng Yang, Yanyi Xing, Wu Moutian, Josep Marcos, Jesús Joglar, Rosa Ventura, Oscar J Pozo
In the fight against doping, the introduction of alternative markers to the steroid profile can be considered as an effective approach to improve the screening capabilities for the detection of testosterone (T) misuse. The aim of this study was to evaluate the potential of several T metabolites (cysteinyl conjugated and glucuronoconjugated resistant to enzymatic hydrolysis) to detect both the transdermal and the intramuscular administration of T. In a first part of the study, we studied the potential of these metabolites for the detection of T transdermal administration...
November 22, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29155490/elimination-profile-of-triamcinolone-in-urine-following-oral-administration
#15
Ting-Ting Chen, Yi-Chun Tseng, Tai-Yuan Huang, Guo-Ping Chang-Chien, Mei-Chich Hsue
Triamcinolone (T) is a glucocorticoid commonly used to relieve inflammation and treat arthritis, severe allergies and asthma; however, it is banned by the World Anti-Doping Agency in competition for athletes when administered orally, intravenously, intramuscularly, or rectally. The minimum required performance limit (MRPL) for urinary T is 30 ng/mL. However, the data about the urinary excretion of T after oral administration is limited. We aimed to investigate the elimination profile and determine whether single-dose administration of T would cause a positive doping result...
November 20, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29151005/synthesis-of-a-novel-polymeric-magnetic-solid-phase-extraction-adsorbent-for-selective-extraction-of-amphetamine-from-urine-samples-coupled-with-high-performance-liquid-chromatography
#16
Arezou Taghvimi, Marjan Ghorbani, Hamed Hamishehkar
A novel pH-responsive block copolymer (Poly ethylene glycol-b-poly (N,N-dimethylaminoethylmethacrylate-co-maleic acid) was designed for the decoration and stabilization of magnetic nanoparticles (MNPs) to apply as an efficient magnetic nano adsorbent for extraction of amphetamine (AM) from biological urine samples to be determined by high performance liquid chromatography ultra violet detector (HPLC-UV). Full characterization of the synthesized Polymeric magnetic nanoparticles (PMNPs) were followed by various techniques like Fourier transform infrared (FT-IR) spectroscopy, powder x-ray diffraction (XRD), scanning electron microscopy (SEM), and vibrating sample magnetometer (VSM)...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150908/10-years-of-drug-testing-and-analysis
#17
EDITORIAL
Paul Trevorrow
No abstract text is available yet for this article.
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150907/sensitivity-of-doping-biomarkers-after-administration-of-a-single-dose-testosterone-gel
#18
Jenny Mullen, Annica Börjesson, Oscar Hopcraft, Jenny J Schulze, Magnus Ericsson, Anders Rane, Mikael Lehtihet, Lena Ekström
Micro-doping with testosterone (T) is challenging to detect with the current doping tests. Today, the methods available to detect T are longitudinally monitoring of urine biomarkers in the Athlete Biological Passport (ABP) and measuring the isotopic composition of excreted biomarkers to distinguish the origin of the molecule. In this study, we investigated the detectability of a single dose of 100 mg T gel in 8 healthy male subjects. We also studied which biomarkers were most sensitive to T gel administration, including blood biomarkers...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29149502/annual-banned-substance-review-analytical-approaches-in-human-sports-drug-testing
#19
Mario Thevis, Tiia Kuuranne, Hans Geyer
A number of high profile revelations concerning anti-doping rule violations over the past 12 months has outlined the importance of tackling prevailing challenges and reducing the limitations of the current anti-doping system. At this time, the necessity to enhance, expand and improve analytical test methods in response to the substances outlined in the World Anti-Doping Agency's (WADA) Prohibited List represents an increasingly crucial task for modern sports drug testing programs. The ability to improve analytical testing methods often relies on the expedient application of novel information regarding superior target analytes for sports drug testing assays, drug elimination profiles, alternative test matrices, together with recent advances in instrumental developments...
November 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29148268/development-and-validation-of-a-uhplc-hrms-ms-quantification-method-for-hypoglycin-a-and-mcpa-carnitine-in-horse-serum-in-cases-of-atypical-myopathy
#20
Wiebke Rudolph, Daniela Remane, Dirk K Wissenbach, Carmen Klein, Dirk Barnewitz, Frank T Peters
Atypical myopathy (AM) is a fatal disease in horses presumably caused by hypoglycin A (HGA) from ingested maple seeds and its active metabolite methylenecyclopropyl-acetic-acid (MCPA). The aim of this study was the development and validation of a rapid and simple assay for HGA and MCPA-carnitine in horse serum and its application to authentic samples. Identification and quantification were carried out by high resolution LC-MS/MS with fullscan/data-dependent MS(2) . Chromatographic separation was performed by isocratic elution on a HILIC column (100 x 2...
November 17, 2017: Drug Testing and Analysis
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