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Drug Testing and Analysis

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https://www.readbyqxmd.com/read/29155490/elimination-profile-of-triamcinolone-in-urine-following-oral-administration
#1
Ting-Ting Chen, Yi-Chun Tseng, Tai-Yuan Huang, Guo-Ping Chang-Chien, Mei-Chich Hsue
Triamcinolone (T) is a glucocorticoid commonly used to relieve inflammation and treat arthritis, severe allergies and asthma; however, it is banned by the World Anti-Doping Agency in competition for athletes when administered orally, intravenously, intramuscularly, or rectally. The minimum required performance limit (MRPL) for urinary T is 30 ng/mL. However, the data about the urinary excretion of T after oral administration is limited. We aimed to investigate the elimination profile and determine whether single-dose administration of T would cause a positive doping result...
November 20, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29151005/synthesis-of-a-novel-polymeric-magnetic-solid-phase-extraction-adsorbent-for-selective-extraction-of-amphetamine-from-urine-samples-coupled-with-high-performance-liquid-chromatography
#2
Arezou Taghvimi, Marjan Ghorbani, Hamed Hamishehkar
A novel pH-responsive block copolymer (Poly ethylene glycol-b-poly (N,N-dimethylaminoethylmethacrylate-co-maleic acid) was designed for the decoration and stabilization of magnetic nanoparticles (MNPs) to apply as an efficient magnetic nano adsorbent for extraction of amphetamine (AM) from biological urine samples to be determined by high performance liquid chromatography ultra violet detector (HPLC-UV). Full characterization of the synthesized Polymeric magnetic nanoparticles (PMNPs) were followed by various techniques like Fourier transform infrared (FT-IR) spectroscopy, powder x-ray diffraction (XRD), scanning electron microscopy (SEM), and vibrating sample magnetometer (VSM)...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150908/10-years-of-drug-testing-and-analysis
#3
EDITORIAL
Paul Trevorrow
No abstract text is available yet for this article.
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150907/sensitivity-of-doping-biomarkers-after-administration-of-a-single-dose-testosterone-gel
#4
Jenny Mullen, Annica Börjesson, Oscar Hopcraft, Jenny J Schulze, Magnus Ericsson, Anders Rane, Mikael Lehtihet, Lena Ekström
Micro-doping with testosterone (T) is challenging to detect with the current doping tests. Today, the methods available to detect T are longitudinally monitoring of urine biomarkers in the Athlete Biological Passport (ABP) and measuring the isotopic composition of excreted biomarkers to distinguish the origin of the molecule. In this study, we investigated the detectability of a single dose of 100 mg T gel in 8 healthy male subjects. We also studied which biomarkers were most sensitive to T gel administration, including blood biomarkers...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29149502/annual-banned-substance-review-analytical-approaches-in-human-sports-drug-testing
#5
Mario Thevis, Tiia Kuuranne, Hans Geyer
A number of high profile revelations concerning anti-doping rule violations over the past 12 months has outlined the importance of tackling prevailing challenges and reducing the limitations of the current anti-doping system. At this time, the necessity to enhance, expand and improve analytical test methods in response to the substances outlined in the World Anti-Doping Agency's (WADA) Prohibited List represents an increasingly crucial task for modern sports drug testing programs. The ability to improve analytical testing methods often relies on the expedient application of novel information regarding superior target analytes for sports drug testing assays, drug elimination profiles, alternative test matrices, together with recent advances in instrumental developments...
November 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29148268/development-and-validation-of-a-uhplc-hrms-ms-quantification-method-for-hypoglycin-a-and-mcpa-carnitine-in-horse-serum-in-cases-of-atypical-myopathy
#6
Wiebke Rudolph, Daniela Remane, Dirk K Wissenbach, Carmen Klein, Dirk Barnewitz, Frank T Peters
Atypical myopathy (AM) is a fatal disease in horses presumably caused by hypoglycin A (HGA) from ingested maple seeds and its active metabolite methylenecyclopropyl-acetic-acid (MCPA). The aim of this study was the development and validation of a rapid and simple assay for HGA and MCPA-carnitine in horse serum and its application to authentic samples. Identification and quantification were carried out by high resolution LC-MS/MS with fullscan/data-dependent MS(2) . Chromatographic separation was performed by isocratic elution on a HILIC column (100 x 2...
November 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29148228/evaluation-of-markers-out-of-the-steroid-profile-for-the-screening-of-testosterone-misuse-part-i-transdermal-administration
#7
Aristotelis Kotronoulas, Àlex Gomez-Gómez, Andreu Fabregat, Jordi Segura, Sheng Yang, Yanyi Xing, Wu Moutian, Josep Marcos, Jesús Joglar, Rosa Ventura, Oscar J Pozo
Although the introduction by WADA of the steroid module of the athlete biological passport (ABP) marked an important step forward in the screening of testosterone (T) misuse, it still remains one of the most difficult challenges in doping control analysis. The urinary determination of alternative markers has been recently reported as a promising tool for improving the screening of T oral administration. However, their evaluation for other, commonly used, administration routes is still required. The main goal of this study is the evaluation of the potential of two groups of metabolites (cysteinyl conjugated and glucuronoconjugated) after transdermal and intramuscular administration of T...
November 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29111580/drug-residues-in-used-syringes-in-switzerland-a-comparative-study
#8
Elodie Lefrançois, Marc Augsburger, Pierre Esseiva
Harm reduction services, including needle exchange programs, have been implemented in Switzerland for over twenty years. Their main aim is to lessen the negative social and/or physical consequences associated with illicit drug consumption and, therefore, improve prevention messages. To this end, knowledge about illicit drug consumption practices is necessary. Periodic self-report surveys are the primary source of data for monitoring drug users' behaviour. Analysis of residual content of used syringes can bring further and objective knowledge about consumed products through analytically confirmed data...
November 7, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29098788/urine-reference-intervals-for-human-chorionic-gonadotropin-hcg-isoforms-by-immunoextraction-tandem-mass-spectrometry-to-detect-hcg-use
#9
Anthony W Butch, Brian D Ahrens, Nuraly K Avliyakulov
Human chorionic gonadotropin (hCG) stimulates testosterone production by the testicles and can normalize suppressed testosterone concentrations in males following prolonged anabolic steroid use. Because of the potential for abuse by males, hCG is on the World Anti-Doping Agency (WADA) list of prohibited substances. The majority of WADA-accredited laboratories measure urinary hCG using an automated immunoassay. Only immunoassays that recognize the intact alpha and beta heterodimer of hCG (intact hCG) should be used to measure urinary hCG for doping control purposes since intact hCG is the only biologically active molecule...
November 3, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29098786/functional-characterization-of-cyp2c19-variants-in-nebivolol-4-hydroxlation-in-vitro
#10
Xiao-Yang Zhou, Xiao-Xia Hu, Meng-Fang Li, Hao Wang, Li-Qun Zhang, Guo-Xin Hu, Jian-Ping Cai
Cytochrome P450 2C19 (CYP2C19) allelic variants are thought to play an important part in inter-individual variability in drug metabolism. We evaluated the in vitro hydroxylation of nebivolol by 31 CYP2C19 alleles identified in a Chinese Han population recently. Wild-type CYP2C19*1B and 30 isoforms were highly expressed in insect cells, and the enzymatic activities of CYP2C19 variants towards nebivolol hydroxylation were characterized. Among the 30 CYP2C19 alleles, most of the recombinant CYP2C19 variants exhibited no or significantly low activity compared with CYP2C19*1B...
November 3, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29088510/a-real-time-in-vitro-assay-to-evaluate-the-release-of-macromolecules-from-liposomes
#11
Himang Mujoo, John N Reynolds, Ian G Tucker
CONTEXT: The availability of a real-time assay to experimentally investigate the release of encapsulated proteins would be beneficial given the interest in the use of liposomes as a drug delivery vehicle. Although simple assays for small molecular weight substances exist, assays to evaluate macromolecules do not. OBJECTIVE: Here we describe a method that detects the release of model macromolecules from liposomes in real-time. METHOD: The assay employs the intermolecular distance-dependent phenomenon of fluorescence resonance energy transfer (FRET) between the fluorophore donor, fluorescein (FITC), and fluorescent quencher, QSY®9...
October 31, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29084416/analysis-for-higenamine-in-urine-by-means-of-uhplc-ms-ms-interpretation-of-results
#12
Krzysztof Grucza, Dorota Kwiatkowska, Katarzyna Kowalczyk, Mariola Wicka, Mirosław Szutowski, Piotr Chołbiński
Higenamine (Norcoclaurine) is a very popular substance in Chinese medicine and it is present in many plants. Moreover, the substance may be found in supplements or nutrients consumption of which may result in violation of anti-doping rules. Higenamine is prohibited in sport at all times and included in class S3 (β-2-agonists) of the World Anti-Doping Agency (WADA) "2017 Prohibited List". Presence of higenamine in urine samples at concentration greater than or equal to 10 ng/mL constitutes an adverse analytical finding (AAF)...
October 30, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29078252/analysis-of-new-psychoactive-substances-in-oral-fluids-by-means-of-microextraction-by-packed-sorbent-followed-by-uhplc-ms-ms
#13
Rachele Rocchi, Maria Chiara Simeoni, Camilla Montesano, Gabriele Vannutelli, Roberta Curini, Manuel Sergi, Dario Compagnone
In recent years, new drugs, commonly known as "new psychoactive substances" (NPS), appeared on the market, which include, among others, synthetic cannabinoids, cathinones and tryptamine analogs of psilocin. The aim of this work was to develop and validate a new method for simultaneous screening and quantification of 31 NPS in oral fluid by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The chosen target analytes represented different chemical and toxicological NPS classes, such as synthetic cathinones, piperazines, phenethylamines, synthetic cannabinoids and their metabolites...
October 27, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29078043/doping-control-analysis-at-the-rio-2016-olympic-and-paralympic-games
#14
Henrique Marcelo Gualberto Pereira, Vinicius Figueiredo Sardela, Monica Costa Padilha, Luciana Mirotti, Alessandro Casilli, Fabio Azamor de Oliveira, Gustavo de Albuquerque Cavalcanti, Lucas Martins Lisandro Rodrigues, Amanda Lessa Dutra de Araujo, Rachel Santos Levy, Pedro Antonio Castelo Teixeira, Felipe Alves Gomes de Oliveira, Ana Carolina Giordani Duarte, Ana Carolina Dudenhoeffer Carneiro, Joseph Albert Medeiros Evaristo, Gustavo Ramalho Cardoso Dos Santos, Giovanni Carlo Verissimo da Costa, Fernando de Lima Castro, Fabio Cesar Sousa Nogueira, Fernanda Bertão Scalco, Luciana Pizzatti, Francisco Radler de Aquino Neto
This paper summarizes the results obtained from the doping control analyses performed during the Summer XXXI Olympic Games (from 03 to 21 August 2016) and XV Paralympic Games (from 07 to 18 September 2016). The analyses of all doping control samples were performed at the Brazilian Doping Control Laboratory (LBCD), a World Anti-Doping Agency (WADA)-accredited laboratory located in Rio de Janeiro, Brazil. A new facility at Rio de Janeiro Federal University (UFRJ) was built and fully operated by over seven hundred professionals, including Brazilian and international scientists, administrative staff and volunteers...
October 27, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29065248/the-35th-manfred-donike-workshop-on-doping-analysis
#15
EDITORIAL
Mario Thevis
No abstract text is available yet for this article.
October 24, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29055108/semi-quantitative-analysis-of-tramadol-dextromethorphan-and-metabolites-in-decomposed-skeletal-tissues-by-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-uplc-qtof-ms
#16
H M Cornthwaite, L Labine, J H Watterson
The use of filtration/pass-through extraction (FPTE) and ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) to detect tramadol (TRAM), dextromethorphan (DXM) and metabolites from skeletal remains is described. Rats (n=5) received 50 mg/kg tramadol and were euthanized by CO2 asphyxiation approximately 30 min post-dose. Rats (n=4) received 75 mg/kg dextromethorphan and were euthanized by CO2 asphyxiation approximately 45 min post-dose. Remains decomposed to skeleton outdoors and vertebral bones were collected...
October 20, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29045058/loop-mediated-isothermal-amplification-lamp-as-an-alternative-to-pcr-a-rapid-on-site-detection-of-gene-doping
#17
Olivier Salamin, Tiia Kuuranne, Martial Saugy, Nicolas Leuenberger
Innovation in medical research has been diverted at multiple occasions to enhance human performance. The predicted great progress in gene therapy has raised some concerns regarding its misuse in the world of sports (gene doping) for several years now. Even though there is no evidence that gene doping has ever been used in sports, the continuous improvement of gene therapy techniques increases the likelihood of abuse. Therefore, since 2004, efforts have been invested by the anti-doping community and WADA for the development of detection methods...
October 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29045074/detection-of-autologous-blood-transfusions-using-a-novel-dried-blood-spot-method
#18
Holly D Cox, Geoffrey D Miller, Auriella Lai, Dan Cushman, Daniel Eichner
In doping control laboratories, autologous blood transfusions are currently detected using an indirect method that monitors changes in an athlete's hemoglobin concentration [Hb] and reticulocyte percent (Ret%) over time. The method is limited by the need for a phlebotomist to collect venous blood and the limited blood stability which requires temperature-controlled shipment and analysis within 72 hours. These limitations significantly reduce the number of samples collected from each athlete and thus the utility of the method...
October 16, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29045066/fatality-involving-ocfentanil-documented-by-identification-of-metabolites
#19
Nathalie Allibe, Camille Richeval, Mélodie Phanithavong, Amandine Faure, Delphine Allorge, François Paysant, Françoise Stanke-Labesque, Hélène Eysseric-Guerin, Jean-Michel Gaulier
New psychoactive substances (NPS) use has rapidly increased over the last decade, and in the last 4 years producers increasingly appear to be targeting non-controlled synthetic opioids, involving fentanyl derivatives such as ocfentanil (OcF). Identification of metabolites is of major importance in the context of NPS use as it could improve detection window in biological matrices in clinical and forensic intoxication cases. Hence, this work aims to report a fatality involving OcF documented by the identification of metabolites...
October 16, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29044958/a-simple-validated-multi-method-for-detecting-drugs-in-oral-fluid-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-uplc-ms-ms
#20
Yufang Zheng, Erik Sparve, Mats Bergström
BACKGROUND: Oral fluid sampling offers several advantages compared to other methods of detecting drugs in biological matrices due to its easy sampling procedure and simple supervision. Hence, the use of oral fluid for drug testing is increasing in workplaces and in roadside testing. As a result, more laboratories are required to perform analyses of drugs in oral fluid. METHODS: A multi-method using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) for tracing drugs in oral fluid was developed...
October 16, 2017: Drug Testing and Analysis
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