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Open Microbiology Journal

Sonia E Fosch, Cecilia A Ficoseco, Antonella Marchesi, Silvina Cocucci, Maria E F Nader-Macias, Beatriz E Perazzi
Background: The vaginal microbiome is influenced by a wide variety of factors, including contraceptive methods. Aim: To evaluate the effect of contraceptive methods on vaginal microbiota and to compare MALDI-TOF MS and 16S rDNA sequencing for lactobacilli identification. Patients and Methods: One hundred and one (101) women consulting for birth control were included in a prospective study. Their vaginal content was sampled and analyzed once before they started using the contraceptive method of their choice, and twice after the initiation of contraception, at three months (94/101 women attended) and at six months (89/101 women attended)...
2018: Open Microbiology Journal
Abebe Sorsa
Introduction: Nowadays various biochemical markers, such as C-Reactive Protein (CRP), Procalcitonin and tumor necrosis factor alpha, have been proposed as a potential marker for screening neonatal sepsis. In the current study, we tried to see the diagnostic significance of White Blood Cell (WBC) count and CRP in diagnostic screening of neonatal sepsis. Methods: A prospective cross-sectional study was conducted from May 2016 to April 2017 in Asella Teaching and Referral Hospital...
2018: Open Microbiology Journal
Tesfaye L Bedada, Firehiwot A Dera, Redwan M Edicho, Samson G Gebre, Yosef B Asefa, Waktole G Sima, Rahel F Maheder, Tigist Y Negassi, Almaz G Biegna
Background: Safe water supply is vital and can result in significant benefits to health. However, contaminated bottled water poses a great health risk due to the poor microbiological quality of water. Methods and Materials: A retrospective study was conducted on 222 Bottled water samples collected from various regions of Ethiopia from January 2008 to December 2015, tested and recorded in Ethiopian Public Health Institute to determine heterotrophic plate count and Staphylococcus aureus by pour plate method; for coliforms using multiple tubes fermentation techniques; for mould and yeast count using spread method, and for Salmonellae and Shigella spp...
2018: Open Microbiology Journal
Mubarak Alfaresi
Introduction: Increased incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been reported worldwide. The WHO warns about the imminent risk to global health if the spread of resistant bacteria is not contained. Materials and Methods: Here, single molecule real time sequencing was used to analyse the whole genome and resistome of SKGH01, a strain of Klebsiella pneumoniae . Results and Discussions: The data showed that SKGH01 was resistant to all commercially available antibiotics...
2018: Open Microbiology Journal
Carolina Arango, Alejandro Acosta-Gonzalez, Claudia M Parra-Giraldo, Zilpa A Sánchez-Quitian, Russell Kerr, Luis E Díaz
Introduction: New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents. Material and Methods: An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach...
2018: Open Microbiology Journal
Mahmudullah Bhuiya, Mohammad K I Sarkar, Mehadi H Sohag, Hafij Ali, Chapol K Roy, Lutfa Akther, Abu F Sarker
Background: Pseudomonas aeruginosa is a ubiquitous free-living bacterium and is responsible for severe nosocomial infections, life-threatening infections in immune compromised persons. The bacterium, along with its natural resistance, can acquire resistance to many antibiotics by a variety of methods. Method: Therefore, to compare the antibiotic sensitivity pattern of Pseudomonas aeruginosa , a total of seventeen isolates of P. aeruginosa were isolated from different sources; for example environmental sources, frozen food sources, clinical sources and medical waste materials...
2018: Open Microbiology Journal
Mohammedaman Mama, Aseer Manilal, Haile Tesfa, Hawa Mohammed, Endeshaw Erbo
Background: Tuberculosis (TB) is an extremely contagious disease detrimentally affecting virtually every organ, most importantly the lungs. Pulmonary complications have been one of the commonest causes of morbidity and mortality since the advent of AIDS (Acquired Immune Deficiency Syndrome) pandemic. The AIDS virus has considerably reshape the epidemiology of TB by widening the risk of reactivating latent TB, increasing the possibility of TB infection once contracted to tubercle bacilli (re-infection) and by elevating the risk of rapid progression instantly after the infection...
2018: Open Microbiology Journal
Zaw K Latt, San S Yu, Ei P Kyaw, Tin M Lynn, May T Nwe, Wai W Mon, Kyaw N Aye
Introduction: Azomonas agilis , a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar. Methods & Materials: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS). Results & Discussion: The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased...
2018: Open Microbiology Journal
Maria Mazzitelli, Angelo G Lamberti, Angela Quirino, Nadia Marascio, Giorgio S Barreca, Chiara Costa, Vincenzo Pisani, Alessio Strazzulla, Giuseppe Greco, Maria C Liberto, Alfredo Focà, Carlo Torti
Background: Bartonella species are intracellular bacteria capable of producing several diseases in humans. The three most common and wellknown diseases are cat scratch disease (CSD), caused by B. henselae, trench fever, caused by B. quintana and Carrion's Disease, caused by B. bacilliformis . Signs and symptoms are very different and aspecific: Fatigue, fever, headache, lymphadenopathy, malaise, loss of weight. No data exist to support guidelines' recommendations to decide which drugs should be optimally used and how long they should be administered...
2018: Open Microbiology Journal
Ruhul Amin, Shafkat S Rahman, Mahboob Hossain, Naiyyum Choudhury
Introduction: Mango juice has always been considered as a delicious, nutritious popular drink, but processed juice may not always be safe due to chemical and microbial risks. Determination of physicochemical and microbiological qualities of some packed mango juices of Bangladesh will help consumers to know the present scenario. Material and Methods: Six commercially available different juice samples were collected from the market. Carbohydrate profiles were determined using HPLC, crude protein content was calculated using the Kjeldahl method and other parameters were determined by standard AOAC methods...
2018: Open Microbiology Journal
Munawwar A Khan, Poojabahen G Patel, Arpitha G Ganesh, Naushad Rais, Sultan M Faheem, Shams T Khan
Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & Materials: Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis...
2018: Open Microbiology Journal
Maysaa El Sayed Zaki, Nermen Abou ElKheir, Mohamed Mofreh
Introduction: Acinetobacterb aumannii ( A. baumannii ) is an important pathogen in health care associated infections. Quinolone resistance has emerged in this pathogen. Aims & Objectives: The aim of the present study was to determine the presence of mutations of gyrA gene and parC genes by Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR) among clinical isolates of A. baumanii . Materials and Methods: The study was carried out on 140 clinical isolates of A...
2018: Open Microbiology Journal
Avijit Das, Sumit Biswas, Malabika Biswas
Background: The Gp07 protein of aureophage Phi11 exhibits growth inhibitory effects when overexpressed in E. coli .The protein harbors two domains- an amino terminal Bro-like domain and a carboxy terminal Ant superfamily like KilA domain, of which the KilA domain retains the growth inhibitory effect of Gp07. Methods: We studied the effects exerted by the overexpression of Gp07 and its separate domains upon the growth rate as well as the morphology of the E. coli cells...
2018: Open Microbiology Journal
Yury Belyi, Ivan Rybolovlev, Nikita Polyakov, Alena Chernikova, Irina Tabakova, Alexandre Gintsburg
Background: Staphylococcus aureus is a Gram-positive bacterium that causes severe illnesses in the human population. The capacity of S. aureus strains to form biofilms on biotic and abiotic surfaces creates serious problems for treatment of hospital infections and has stimulated efforts to develop new means of specific protection or immunotherapy. Material and Methods: We found that rabbit serum raised against crude concentrated S. aureus liquid culture significantly decreased the development of staphylococcal biofilm in vitro ...
2018: Open Microbiology Journal
Davood Azadi, Tahereh Motallebirad, Kazem Ghaffari, Hasan Shojaei
Background: Tuberculosis is one of the most important infectious diseases that has claimed its victims throughout much of known human history. With Koch's discovery of the tubercle bacillus as the etiologic agent of the disease, his sanitary and hygienic measures, which were based on his discovery and the development of a vaccine against tuberculosis by Albert Calmette and Camille Guérin in 1921, an attenuated Mycobacterium bovis strain, bacilli Calmette-Guérin (BCG), and the discovery of the first antibiotic against tuberculosis, streptomycin by Selman Waksman in 1943, soon led to the opinion that appropriate control measures had become available for tuberculosis and it had been assumed that the disease could ultimately be eradicated...
2018: Open Microbiology Journal
Salma M Al-Zain Ahmed, Sara S Abdelrahman, Doua M Saad, Isra S Osman, Modasir G Osman, Eltahir A G Khalil
Background: Respiratory infections are one of the commonest causes of morbidity and mortality related to infectious diseases worldwide. The emergence of antimicrobial resistance is a major global health problem which is well established in developing countries. Good clinical suspicion and correct laboratory identification of respiratory infection causing organisms followed by the appropriate management are needed to compact both community-acquired and nosocomial infection respiratory infections...
2018: Open Microbiology Journal
Anusha Gopinathan, Anil Kumar, Amitabh C Sen, Srisruthy Sudha, Praveen Varma, Sunil Gs, Malini Eapen, Kavitha R Dinesh
Introduction: Bacillus cereus is a gram positive bacilli found commonly in the soil and environment. It is a bacteria rarely associated with endocarditis. Case History: Intravenous drug abuse, presence of valvular defects, pacemakers, immunodeficiency are some of the known risk factors for B.cereus endocarditis. We present here a case series of two patients with B.cereus endocarditis along with a review of the literature. Conclusion: This is the first report of B...
2018: Open Microbiology Journal
Maxime Even, Stéphane Davail, Mikael Rey, Annabelle Tavernier, Marianne Houssier, Marie Dominique Bernadet, Karine Gontier, Géraldine Pascal, Karine Ricaud
Background: Livestock production should respond to societal, environmental and economic changes. Since 2006 and the ban on antibiotics as growth factors in European Union, the use of probiotics has become widespread and has demonstrated the effect of intestinal microbiota on the performance of farm animals. Objective: The aim of this study was to investigate the effect of supplementation with Lactobacillus salivarius (as a probiotics strain or combined with other strains) on zootechnical performance, metabolic and immune gene expression and intestinal microbiota diversity in mule ducks using high-throughput sequencing and real-time PCR...
2018: Open Microbiology Journal
Zahra Karimi, Ali Ahmadi, Ali Najafi, Reza Ranjbar
Introduction: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci as novel and applicable regions in prokaryotic genomes have gained great attraction in the post genomics era. Methods: These unique regions are diverse in number and sequence composition in different pathogenic bacteria and thereby can be a suitable candidate for molecular epidemiology and genotyping studies. Results:Furthermore, the arrayed structure of CRISPR loci (several unique repeats spaced with the variable sequence) and associated cas genes act as an active prokaryotic immune system against viral replication and conjugative elements...
2018: Open Microbiology Journal
Joshua N Edokpayi, John O Odiyo, Elizabeth O Popoola, Titus A M Msagati
Background: Access to clean and safe drinking water is still a problem in developing countries and more pronounced in rural areas. Due to erratic supply of potable, rural dwellers often seek for an alternative source of water to meet their basic water needs. The objective of this study is to monitor the microbiological and physicochemical water quality parameters of Nzhelele River which is a major alternative source of drinking water to villages along its course in Limpopo province of South Africa...
2018: Open Microbiology Journal
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