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Open Microbiology Journal

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https://www.readbyqxmd.com/read/29785220/physicochemical-and-microbiological-qualities-assessment-of-popular-bangladeshi-mango-fruit-juice
#1
Ruhul Amin, Shafkat S Rahman, Mahboob Hossain, Naiyyum Choudhury
Introduction: Mango juice has always been considered as a delicious, nutritious popular drink, but processed juice may not always be safe due to chemical and microbial risks. Determination of physicochemical and microbiological qualities of some packed mango juices of Bangladesh will help consumers to know the present scenario. Material and Methods: Six commercially available different juice samples were collected from the market. Carbohydrate profiles were determined using HPLC, crude protein content was calculated using the Kjeldahl method and other parameters were determined by standard AOAC methods...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785219/assessing-methanogenic-archaeal-community-in-full-scale-anaerobic-sludge-digester-systems-in-dubai-united-arab-emirates
#2
Munawwar A Khan, Poojabahen G Patel, Arpitha G Ganesh, Naushad Rais, Sultan M Faheem, Shams T Khan
Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & Materials: Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785218/molecular-study-of-quinolone-resistance-determining-regions-of-gyra-gene-and-parc-gene-s-in-clinical-isolates-of-acintobacter-baumannii-resistant-to-fluoroquinolone
#3
Maysaa El Sayed Zaki, Nermen Abou ElKheir, Mohamed Mofreh
Introduction: Acinetobacterb aumannii ( A. baumannii ) is an important pathogen in health care associated infections. Quinolone resistance has emerged in this pathogen. Aims & Objectives: The aim of the present study was to determine the presence of mutations of gyrA gene and parC genes by Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR) among clinical isolates of A. baumanii . Materials and Methods: The study was carried out on 140 clinical isolates of A...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785217/expression-of-phi11-gp07-causes-filamentation-in-escherichia-coli
#4
Avijit Das, Sumit Biswas, Malabika Biswas
Background: The Gp07 protein of aureophage Phi11 exhibits growth inhibitory effects when overexpressed in E. coli .The protein harbors two domains- an amino terminal Bro-like domain and a carboxy terminal Ant superfamily like KilA domain, of which the KilA domain retains the growth inhibitory effect of Gp07. Methods: We studied the effects exerted by the overexpression of Gp07 and its separate domains upon the growth rate as well as the morphology of the E. coli cells...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785216/-staphylococcus-aureus-surface-protein-g-is-an-immunodominant-protein-and-a-possible-target-in-an-anti-biofilm-drug-development
#5
Yury Belyi, Ivan Rybolovlev, Nikita Polyakov, Alena Chernikova, Irina Tabakova, Alexandre Gintsburg
Background: Staphylococcus aureus is a Gram-positive bacterium that causes severe illnesses in the human population. The capacity of S. aureus strains to form biofilms on biotic and abiotic surfaces creates serious problems for treatment of hospital infections and has stimulated efforts to develop new means of specific protection or immunotherapy. Material and Methods: We found that rabbit serum raised against crude concentrated S. aureus liquid culture significantly decreased the development of staphylococcal biofilm in vitro ...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785215/mycobacteriosis-and-tuberculosis-laboratory-diagnosis
#6
REVIEW
Davood Azadi, Tahereh Motallebirad, Kazem Ghaffari, Hasan Shojaei
Background: Tuberculosis is one of the most important infectious diseases that has claimed its victims throughout much of known human history. With Koch's discovery of the tubercle bacillus as the etiologic agent of the disease, his sanitary and hygienic measures, which were based on his discovery and the development of a vaccine against tuberculosis by Albert Calmette and Camille Guérin in 1921, an attenuated Mycobacterium bovis strain, bacilli Calmette-Guérin (BCG), and the discovery of the first antibiotic against tuberculosis, streptomycin by Selman Waksman in 1943, soon led to the opinion that appropriate control measures had become available for tuberculosis and it had been assumed that the disease could ultimately be eradicated...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785214/etiological-trends-and-patterns-of-antimicrobial-resistance-in-respiratory-infections
#7
Salma M Al-Zain Ahmed, Sara S Abdelrahman, Doua M Saad, Isra S Osman, Modasir G Osman, Eltahir A G Khalil
Background: Respiratory infections are one of the commonest causes of morbidity and mortality related to infectious diseases worldwide. The emergence of antimicrobial resistance is a major global health problem which is well established in developing countries. Good clinical suspicion and correct laboratory identification of respiratory infection causing organisms followed by the appropriate management are needed to compact both community-acquired and nosocomial infection respiratory infections...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785213/a-case-series-and-review-of-bacillus-cereus-endocarditis-from-india
#8
Anusha Gopinathan, Anil Kumar, Amitabh C Sen, Srisruthy Sudha, Praveen Varma, Sunil Gs, Malini Eapen, Kavitha R Dinesh
Introduction: Bacillus cereus is a gram positive bacilli found commonly in the soil and environment. It is a bacteria rarely associated with endocarditis. Case History: Intravenous drug abuse, presence of valvular defects, pacemakers, immunodeficiency are some of the known risk factors for B.cereus endocarditis. We present here a case series of two patients with B.cereus endocarditis along with a review of the literature. Conclusion: This is the first report of B...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29755604/probiotics-strains-modulate-gut-microbiota-and-lipid-metabolism-in-mule-ducks
#9
Maxime Even, Stéphane Davail, Mikael Rey, Annabelle Tavernier, Marianne Houssier, Marie Dominique Bernadet, Karine Gontier, Géraldine Pascal, Karine Ricaud
Background: Livestock production should respond to societal, environmental and economic changes. Since 2006 and the ban on antibiotics as growth factors in European Union, the use of probiotics has become widespread and has demonstrated the effect of intestinal microbiota on the performance of farm animals. Objective: The aim of this study was to investigate the effect of supplementation with Lactobacillus salivarius (as a probiotics strain or combined with other strains) on zootechnical performance, metabolic and immune gene expression and intestinal microbiota diversity in mule ducks using high-throughput sequencing and real-time PCR...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29755603/bacterial-crispr-regions-general-features-and-their-potential-for-epidemiological-molecular-typing-studies
#10
REVIEW
Zahra Karimi, Ali Ahmadi, Ali Najafi, Reza Ranjbar
Introduction: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci as novel and applicable regions in prokaryotic genomes have gained great attraction in the post genomics era. Methods: These unique regions are diverse in number and sequence composition in different pathogenic bacteria and thereby can be a suitable candidate for molecular epidemiology and genotyping studies. Results:Furthermore, the arrayed structure of CRISPR loci (several unique repeats spaced with the variable sequence) and associated cas genes act as an active prokaryotic immune system against viral replication and conjugative elements...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29541268/evaluation-of-microbiological-and-physicochemical-parameters-of-alternative-source-of-drinking-water-a-case-study-of-nzhelele-river-south-africa
#11
Joshua N Edokpayi, John O Odiyo, Elizabeth O Popoola, Titus A M Msagati
Background: Access to clean and safe drinking water is still a problem in developing countries and more pronounced in rural areas. Due to erratic supply of potable, rural dwellers often seek for an alternative source of water to meet their basic water needs. The objective of this study is to monitor the microbiological and physicochemical water quality parameters of Nzhelele River which is a major alternative source of drinking water to villages along its course in Limpopo province of South Africa...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29541267/programmatic-impact-of-implementing-genexpert-mtb-rif-assay-for-the-detection-of-mycobacterium-tuberculosis-in-respiratory-specimens-from-pulmonary-tuberculosis-suspected-patients-in-resource-limited-laboratory-settings-of-eastern-nepal
#12
Prakash Shrestha, Hemanta Khanal, Prasanna Dahal, Pranita Dongol
Background: In Nepal, introduction of GeneXpert MTB/RIF assay (Xpert assay) as an initial confirmation test for tuberculosis (TB) has been considered to have impact as a significant decrease in number of clinically diagnosed pulmonary tuberculosis (PTB) cases than previous years. This study aims to find out the distribution profile of suspected tuberculosis cases according to patients age, gender, treatment history and HIV status as well as to evaluate the utility of the Xpert assay over conventional acid-fast bacilli (AFB) staining method for the proper diagnosis of M...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29541266/laser-optofluidics-in-fighting-multiple-drug-resistance
#13
REVIEW
Thodoris D Karapantsios
No abstract text is available yet for this article.
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29456773/molecular-characterization-of-multidrug-resistant-uropathogenic-e-coli-isolates-from-jordanian-patients
#14
Yacoub R Nairoukh, Azmi M Mahafzah, Amal Irshaid, Asem A Shehabi
Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29399219/identification-of-bacteria-in-the-sputum-of-a-cystic-fibrosis-patient-a-comparison-of-phenotypic-and-molecular-methods
#15
Mubarak Alfaresi, Bassam Mahboub
Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29399218/antimicrobial-activity-of-bacillus-persicus-24-dsm-isolated-from-dead-sea-mud
#16
Nehaya Al-Karablieh
Intorduction: Dead Sea is a hypersaline lake with 34% salinity, gains its name due to the absence of any living macroscopic creatures. Despite the extreme hypersaline environment, it is a unique ecosystem for various halophilic microorganisms adapted to this environment. Aims & Objectives: Halophilic microorganisms are known for various potential biotechnological applications, the purpose of the current research is isolation and screening of halophilic bacteria from Dead Sea mud for potential antimicrobial applications...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29399217/epidemiology-of-clostridium-difficile-infection-in-a-large-hospital-in-northern-italy-questioning-the-ward-based-transmission
#17
Gabriella Piatti, Marco Bruzzone, Vincenzo Fontana, Alessandro Mannini, Marcello Ceppi
Background: Clostridium Difficile infection (CDI) is considered a ward-based nosocomial infection, due to contagion among patients. Molecular studies recently questioned ward-based contact for disease spread. Objective: To investigate whether it is plausible that CDI spread in San Martino Hospital of Genoa was due to a ward-based contact and patient-to-patient diffusion. Methods: We conducted a retrospective cohort study of CDI cases from April 2010 to March 2015...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29399216/-in-vitro-antibacterial-activity-and-phytochemical-analysis-of-nicotiana-tabacum-l-extracted-in-different-organic-solvents
#18
Gemechu Ameya, Aseer Manilal, Behailu Merdekios
Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29290846/trends-in-the-genetic-background-of-methicillin-resistant-staphylococcus-aureus-clinical-isolates-in-a-south-african-hospital-an-institutional-based-observational-study
#19
John F Antiabong, Marleen M Kock, Tsidiso G Maphanga, Adeola M Salawu, Nontombi M Mbelle, Marthie M Ehlers
Background: This study sought to understand the epidemio-ecological dynamics of MRSA isolates associated with a South African hospital over a period spanning year 2007-8 (a previous study reported in 2009) and year 2010-11 (this study). Methods: One hundred and ninety three isolates were characterised by molecular fingerprinting methods including pulsed field gel electrophoresis (PFGE), spa typing, agr -typing, SCC mec -typing, and multilocus sequence typing (MLST)...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29290845/dna-microarray-for-rapid-detection-and-identification-of-food-and-water-borne-bacteria-from-dry-to-wet-lab
#20
Reza Ranjbar, Payam Behzadi, Ali Najafi, Raheleh Roudi
Background: A rapid, accurate, flexible and reliable diagnostic method may significantly decrease the costs of diagnosis and treatment. Designing an appropriate microarray chip reduces noises and probable biases in the final result. Objective: The aim of this study was to design and construct a DNA Microarray Chip for a rapid detection and identification of 10 important bacterial agents. Method: In the present survey, 10 unique genomic regions relating to 10 pathogenic bacterial agents including Escherichia coli (E...
2017: Open Microbiology Journal
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