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Open Microbiology Journal

Karine Ricaud, Mickael Rey, Elisabeth Plagnes-Juan, Laurence Larroquet, Maxime Even, Edwige Quillet, Sandrine Skiba-Cassy, Stéphane Panserat
Background: Recently, studies suggest that gut microbiota contributes to the development of obesity in mammals. In rainbow trout, little is known about the role of intestinal microbiota in host physiology. Objective: The aim of this study was to investigate the link between intestinal microbiota and adiposity, by high-throughput 16S RNA gene based illumina Miseq sequencing in two rainbow trout lines divergently selected for muscle lipid content. Fish from these two lines of rainbow trout are known to have a differing lipid metabolism...
2018: Open Microbiology Journal
Leonila M L Acioly, Davi Cavalcanti, Marcos C Luna, José C V Júnior, Rosileide F S Andrade, Thayse A de Lima E Silva, Camilo E La Rotta, Galba M Campos-Takaki
Background: Cadmium (Cd), which is a deadly heavy metal of work-related and environmental concern, has been recognized as a substance that is teratogenic and carcinogenic for humans. Therefore, the need to develop low-cost adsorbents to remove heavy metals from aqueous solution has greatly increased. Adsorbents such as Pantoea agglomerans biomass have been used. Aims: We investigated the biotechnological potential of Pantoea agglomerans for the biosorption of cadmium from aqueous solution...
2018: Open Microbiology Journal
Sónia G Pereira, Vanessa S Domingues, João Theriága, Maria de Jesus Chasqueira, Paulo Paixão
Introduction: Multiple-drug resistant bacteria are emerging exponentially in healthcare units, threatening public health and requiring novel therapeutic approaches. In 2017, World Health Organization published a list that frames antimicrobial resistant bacteria into priority levels for research of novel drugs to fight them. Methods & Materials: Antimicrobial resistant ESKAPE ( Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , Enterobacter sp...
2018: Open Microbiology Journal
Tahar Hazzaz Abouamal, Zineb Choukairi, Fechtali Taoufiq
Introduction: T-type Ca2+ channels (TTCC) are low Voltage-gated calcium channels, expressed in various tissues such as the brain and heart, and contribute to a variety of physiological functions including neuronal excitability, hormone secretion, muscle contraction, and pacemaker activity. At high concentrations, Zinc (Zn2+ ) is naturally attached to cell membranes and is therefore considered a reversible inhibitor of calcium. Zinc is also involved in the kinetics of sodium and potassium currents...
2018: Open Microbiology Journal
Nicholas O Igiehon, Olubukola O Babalola
Introduction: Organisms seldom exist in isolation and are usually involved in interactions with several hosts and these interactions in conjunction with the physicochemical parameters of the soil affect plant growth and development. Researches into below and aboveground microbial community are unveiling a myriad of intriguing interactions within the rhizosphere, and many of the interactions are facilitated by exudates that are secreted by plants roots. These interactions can be harnessed for beneficial use in agriculture to enhance crop productivity especially in semi-arid and arid environments...
2018: Open Microbiology Journal
Reza Ranjbar, Sajjad S Tolon, Shahin Zayeri, Mehrdad Sami
Background: Bacterial resistance to antibiotics limits treatment options, increases morbidity and mortality, and raises the risk of antibiotic-associated adverse events. Antibacterial resistance emerges rapidly following an increase in the consumption of antibiotics against infectious diseases. The spread of ESBL producing strains has a limiting factor based on antibiotic function for the treatment of infections particularly caused by Acinetobacter baumannii ( A. baumannii ). Objective: This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution of blaTEM , blaCTX , and blaSHV genes among A...
2018: Open Microbiology Journal
Reza Ranjbar, Sajjad S Tolon, Mehrdad Sami, Reza Golmohammadi
Background: Escherichia coli is one of the most important bacterial agents to cause urinary tract infections. Inappropriate and unnecessary administration of antibiotics has led to an increase in the appearance of multidrug-resistant E. coli isolates, limiting treatment options. The increase in a number of resistant strains of bacteria is a major concern of health authorities worldwide. Objective: The purpose of this study was to determine the presence of the qnr genes among E...
2018: Open Microbiology Journal
Marianne Stærk, Sara A Tolouee, Jens J Christensen
Introduction: Haemophilus influenzae commonly causes upper respiratory tract infections and has only rarely been reported etiology of urinary tract infections. Since the introduction of the Haemophilus influenzae b (Hib) vaccine, non-typable haemophilus species now cause the majority of invasive disease in Europe. Case Report: We report a case of an adult man with non-typable Haemophilus influenzae septicemia, urinary tract infection and bilateral renal stone disease...
2018: Open Microbiology Journal
Ram H Dahal, Dhiraj K Chaudhary
Antimicrobial resistance is a life threatening challenges to the world. Most of the well-known antibiotics are currently ineffective to several microbial diseases. Ampicillin, metronidazole, amoxicillin, cotrimoxazole, chloramphenicol, ciprofloxacin, nalidixic acid, gentamicin, and ceftazidime are common antibiotics whose resistance pattern has been elevated in recent years. The rise and dissemination of resistant bacteria has contributed in increasing cases of antimicrobial resistance. Multi-drug Resistant (MDR) organism such as Staphylococcus aureus, Pseudomionas aeruginosa, Escherchia coli , and Mycobacterium tuberculosis are principal problems for public health and stakeholders...
2018: Open Microbiology Journal
Sonia E Fosch, Cecilia A Ficoseco, Antonella Marchesi, Silvina Cocucci, Maria E F Nader-Macias, Beatriz E Perazzi
Background: The vaginal microbiome is influenced by a wide variety of factors, including contraceptive methods. Aim: To evaluate the effect of contraceptive methods on vaginal microbiota and to compare MALDI-TOF MS and 16S rDNA sequencing for lactobacilli identification. Patients and Methods: One hundred and one (101) women consulting for birth control were included in a prospective study. Their vaginal content was sampled and analyzed once before they started using the contraceptive method of their choice, and twice after the initiation of contraception, at three months (94/101 women attended) and at six months (89/101 women attended)...
2018: Open Microbiology Journal
Abebe Sorsa
Introduction: Nowadays various biochemical markers, such as C-Reactive Protein (CRP), Procalcitonin and tumor necrosis factor alpha, have been proposed as a potential marker for screening neonatal sepsis. In the current study, we tried to see the diagnostic significance of White Blood Cell (WBC) count and CRP in diagnostic screening of neonatal sepsis. Methods: A prospective cross-sectional study was conducted from May 2016 to April 2017 in Asella Teaching and Referral Hospital...
2018: Open Microbiology Journal
Tesfaye L Bedada, Firehiwot A Dera, Redwan M Edicho, Samson G Gebre, Yosef B Asefa, Waktole G Sima, Rahel F Maheder, Tigist Y Negassi, Almaz G Biegna
Background: Safe water supply is vital and can result in significant benefits to health. However, contaminated bottled water poses a great health risk due to the poor microbiological quality of water. Methods and Materials: A retrospective study was conducted on 222 Bottled water samples collected from various regions of Ethiopia from January 2008 to December 2015, tested and recorded in Ethiopian Public Health Institute to determine heterotrophic plate count and Staphylococcus aureus by pour plate method; for coliforms using multiple tubes fermentation techniques; for mould and yeast count using spread method, and for Salmonellae and Shigella spp...
2018: Open Microbiology Journal
Mubarak Alfaresi
Introduction: Increased incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been reported worldwide. The WHO warns about the imminent risk to global health if the spread of resistant bacteria is not contained. Materials and Methods: Here, single molecule real time sequencing was used to analyse the whole genome and resistome of SKGH01, a strain of Klebsiella pneumoniae . Results and Discussions: The data showed that SKGH01 was resistant to all commercially available antibiotics...
2018: Open Microbiology Journal
Carolina Arango, Alejandro Acosta-Gonzalez, Claudia M Parra-Giraldo, Zilpa A Sánchez-Quitian, Russell Kerr, Luis E Díaz
Introduction: New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents. Material and Methods: An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach...
2018: Open Microbiology Journal
Mahmudullah Bhuiya, Mohammad K I Sarkar, Mehadi H Sohag, Hafij Ali, Chapol K Roy, Lutfa Akther, Abu F Sarker
Background: Pseudomonas aeruginosa is a ubiquitous free-living bacterium and is responsible for severe nosocomial infections, life-threatening infections in immune compromised persons. The bacterium, along with its natural resistance, can acquire resistance to many antibiotics by a variety of methods. Method: Therefore, to compare the antibiotic sensitivity pattern of Pseudomonas aeruginosa , a total of seventeen isolates of P. aeruginosa were isolated from different sources; for example environmental sources, frozen food sources, clinical sources and medical waste materials...
2018: Open Microbiology Journal
Mohammedaman Mama, Aseer Manilal, Haile Tesfa, Hawa Mohammed, Endeshaw Erbo
Background: Tuberculosis (TB) is an extremely contagious disease detrimentally affecting virtually every organ, most importantly the lungs. Pulmonary complications have been one of the commonest causes of morbidity and mortality since the advent of AIDS (Acquired Immune Deficiency Syndrome) pandemic. The AIDS virus has considerably reshape the epidemiology of TB by widening the risk of reactivating latent TB, increasing the possibility of TB infection once contracted to tubercle bacilli (re-infection) and by elevating the risk of rapid progression instantly after the infection...
2018: Open Microbiology Journal
Zaw K Latt, San S Yu, Ei P Kyaw, Tin M Lynn, May T Nwe, Wai W Mon, Kyaw N Aye
Introduction: Azomonas agilis , a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar. Methods & Materials: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS). Results & Discussion: The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased...
2018: Open Microbiology Journal
Maria Mazzitelli, Angelo G Lamberti, Angela Quirino, Nadia Marascio, Giorgio S Barreca, Chiara Costa, Vincenzo Pisani, Alessio Strazzulla, Giuseppe Greco, Maria C Liberto, Alfredo Focà, Carlo Torti
Background: Bartonella species are intracellular bacteria capable of producing several diseases in humans. The three most common and wellknown diseases are cat scratch disease (CSD), caused by B. henselae, trench fever, caused by B. quintana and Carrion's Disease, caused by B. bacilliformis . Signs and symptoms are very different and aspecific: Fatigue, fever, headache, lymphadenopathy, malaise, loss of weight. No data exist to support guidelines' recommendations to decide which drugs should be optimally used and how long they should be administered...
2018: Open Microbiology Journal
Ruhul Amin, Shafkat S Rahman, Mahboob Hossain, Naiyyum Choudhury
Introduction: Mango juice has always been considered as a delicious, nutritious popular drink, but processed juice may not always be safe due to chemical and microbial risks. Determination of physicochemical and microbiological qualities of some packed mango juices of Bangladesh will help consumers to know the present scenario. Material and Methods: Six commercially available different juice samples were collected from the market. Carbohydrate profiles were determined using HPLC, crude protein content was calculated using the Kjeldahl method and other parameters were determined by standard AOAC methods...
2018: Open Microbiology Journal
Munawwar A Khan, Poojabahen G Patel, Arpitha G Ganesh, Naushad Rais, Sultan M Faheem, Shams T Khan
Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & Materials: Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis...
2018: Open Microbiology Journal
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