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Open Microbiology Journal

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https://www.readbyqxmd.com/read/28932329/molecular-characterization-of-klebsiella-pneumoniae-clinical-isolates-with-elevated-resistance-to-carbapenems
#1
Rasha Barwa, Mona Shaaban
BACKGROUND: Emergence of carbapenems-resistant K. pneumoniae represents a serious challenge for antimicrobial therapy. OBJECTIVE: The aim of this research is to determine different mechanisms mediating the emergence of K. pneumoniae isolates with high-level carbapenem resistance. METHOD: A total of 80 K. pneumoniae isolates were purified from sputum and urine specimens. The minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by broth microdilution method...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28839494/sub-inhibitory-concentrations-of-rifampicin-strongly-stimulated-biofilm-production-in-s-aureus
#2
Agostinho Alves Lima-E-Silva, Renato Geraldo Silva-Filho, Henry Marcel Zalona Fernandes, Carmen Soares Meirelles Saramago, Alice Slotfeldt Viana, Maria José Souza, Eduardo Matos Nogueira
BACKGROUND AND OBJECTIVES: Staphylococcus aureus is an important pathogen and a frequent cause of infections associated with biofilm production in implantable medical devices. Biofilm production can be induced by sub-inhibitory concentrations (sub-MICs) of certain antibiotics, but few studies have researched this occurrence in S. aureus. In this study, we investigated the effect of sub-MICs of rifampicin and minocycline on biofilm production by five clinical and five non-clinical S. aureus isolates...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28839493/diversity-of-multidrug-efflux-genes-and-phenotypic-evaluation-of-the-in-vitro-resistance-dynamics-of-clinical-staphylococcus-aureus-isolates-using-methicillin-a-model-%C3%AE-lactam
#3
John F Antiabong, Marleen M Kock, Nontombi M Bellea, Marthie M Ehlers
OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) across the world often leave clinicians with little or no choice of treatment options. The multi-drug efflux (MDE) genes are bacterial survival mechanisms responsible for the pumping out of antibiotics and other biocides from the cytoplasm. Whilst effort is being made in the development of antibiotic adjuvants such as efflux pumps inhibitors, information is needed on the diversity of these MDEs in the circulating S. aureus and on the growth dynamics of the clinical isolates in response to antibiotics is not regularly examined...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28839492/misinterpretation-of-gram-stain-from-the-stationary-growth-phase-of-positive-blood-cultures-for-brucella-and-acinetobacter-species
#4
Ali M Bazzi, Jaffar A Al-Tawfiq, Ali A Rabaan
INTRODUCTION: Acinetobacter baumannii and Brucella species are Gram-negative organisms that are vulnerable to misinterpretation as Gram-positive or Gram-variable in blood cultures. OBJECTIVE: We assess the random errors in gram stain interpretation to reduce the likelihood of such errors and therefore patient harm. METHODOLOGY: Aerobic and anaerobic blood cultures from two patients in an acute care facility in Saudi Arabia were subjected to preliminary Gram-staining...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28839491/non-coding-rnas-are-differentially-expressed-by-nocardia-brasiliensis-in-vitro-and-in-experimental-actinomycetoma
#5
Josué S Cruz-Rabadán, Juan Miranda-Ríos, Guadalupe Espín-Ocampo, Luis J Méndez-Tovar, Héctor Rubén Maya-Pineda, Francisca Hernández-Hernández
INTRODUCTION: Nocardia spp. are common soil-inhabiting bacteria that frequently infect humans through traumatic injuries or inhalation routes and cause infections, such as actinomycetoma and nocardiosis, respectively. Nocardia brasiliensis is the main aetiological agent of actinomycetoma in various countries. Many bacterial non-coding RNAs are regulators of genes associated with virulence factors. OBJECTIVE: The aim of this work was to identify non-coding RNAs (ncRNAs) expressed during infection conditions and in free-living form (in vitro) in Nocardia brasiliensis...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28839490/effects-of-prenatal-consumption-of-caprine-milk-oligosaccharides-on-mice-mono-associated-with-bifidobacterium-bifidum-agr2166
#6
Caroline Thum, Kikuji Itoh, Wayne Young, Adrian Cookson, Warren McNabb, Nicole Roy
BACKGROUND: Prenatal consumption of oligosaccharides are associated with changes in the maternal gastrointestinal tract (GIT) microbiota with health consequences for the offspring. It has previously been demonstrated that caprine milk oligosaccharides (CMO) stimulate the growth and fermentation rate of Bifidobacterium bifidum AGR2166. OBJECTIVE: The objective of this study was to examine the effects of B. bifidum AGR2166 and prenatal consumption of CMO, alone or in combination, on the dam's large intestine, foetal development and ability of B...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28761562/assessment-of-physicochemical-and-microbiological-quality-of-public-swimming-pools-in-addis-ababa-ethiopia
#7
Kokebe Yedeme, Melese Hailu Legese, Almaz Gonfa, Somson Girma
BACKGROUND: From swimming pools, bathers may acquire many potential pathogens or may be affected by the physicochemical characteristics of water used during bathing. Hence, this study aimed at assessing the physicochemical and microbiological quality of public swimming pools located at different hotels and recreation center in Addis Ababa, Ethiopia. METHOD: A cross sectional study was carried out from February to May, 2016. Nine hotels and one recreation center which recognized to have public swimming services were included...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28659996/the-immunomodulatory-effect-of-trichophyton-rubrum-exoantigens-in-the-treatment-of-experimental-septic-arthritis
#8
Seyed A Ghiasian, Amir H Maghsood, Asadollah Abniki, Abbas Mirshafiey
BACKGROUND: Understanding the nature and function of fungal exoantigens might lead to novel approaches in the treatment and prophylaxis of some infectious diseases. Septic arthritis represents a serious problem for medicine due to the high incidence rate and severe complications. OBJECTIVE: The present study aimed at assessing the immunomodulatory effects of Trichophyton rubrum culture filtrate as a novel compound in experimental septic arthritis. METHOD: The septic arthritis was haematogenously induced in Sprague-Dawley rats by a single intravenous injection of 10(9) colony forming units of the human clinical isolate Staphylococcus aureus producing toxic shock syndrome toxin-1...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28603564/a-study-on-the-microbiological-status-of-mineral-drinking-water
#9
Faria Y Aditi, Shafkat S Rahman, Md M Hossain
INTRODUCTION: Water-borne diseases constitute a major health burden in Bangladesh. The objective of this study was to assess the overall quality of mineral water samples that obtained from different shops of Dhaka city. MATERIAL AND METHODS: To achieve the above-mentioned objective, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated colony from mineral water samples were characterized by using biochemical and antimicrobial susceptibility tests...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28567147/ozone-sensitivity-and-catalase-activity-in-pigmented-and-non-pigmented-strains-of-serratia-marcescens
#10
José de Ondarza
BACKGROUND: Ozone exposure rapidly leads to bacterial death, making ozone an effective disinfectant in food industry and health care arena. However, microbial defenses may moderate this effect and play a role in the effective use of oxidizing agents for disinfection. Serratia marcescens is an opportunistic pathogen, expressing genes differentially during infection of a human host. A better understanding of regulatory systems that control expression of Serratia's virulence genes and defenses is therefore valuable...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28567146/virulence-factors-associated-with-enterococcus-faecalis-infective-endocarditis-a-mini-review
#11
REVIEW
Kristian T Madsen, Marianne N Skov, Sabine Gill, Michael Kemp
INTRODUCTION: The enterococci are accountable for up to 20% of all cases of infective endocarditis, with Enterococcus faecalis being the primary causative isolate. Infective endocarditis is a life-threatening infection of the endocardium that results in the formation of vegetations. Based on a literature review, this paper provides an overview of the virulence factors associated with E. faecalis infective endocarditis. Furthermore, it reports the effects of active or passive immunization against some of these involved factors...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28553417/synergistic-effect-of-colistin-and-rifampin-against-multidrug-resistant-acinetobacter-baumannii-a-systematic-review-and-meta-analysis
#12
REVIEW
Maryam Mohammadi, Hatef Khayat, Koroush Sayehmiri, Setareh Soroush, Fatemeh Sayehmiri, Somayeh Delfani, Lidija Bogdanovic, Morovat Taherikalani
The existence of infections caused by multidrug resistant (MDR) Acinetobacter baumannii is a growing problem because of the difficulty to treat them. We examined the published literature and focused our analysis on the investigation of the synergism of colistin and rifampin against MDR A. baumannii isolates via systematic review and meta-analysis. A systematic literature search was performed using the following 4 databases (PubMed, Scopus, EMBASE and ISI Web of Sciences). The related articles were evaluated during the period from December 2014 to January 2015...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28553416/understanding-the-mechanism-of-bacterial-biofilms-resistance-to-antimicrobial-agents
#13
REVIEW
Shriti Singh, Santosh Kumar Singh, Indrajit Chowdhury, Rajesh Singh
A biofilm is a group of microorganisms, that causes health problems for the patients with indwelling medical devices via attachment of cells to the surface matrix. It increases the resistance of a microorganism for antimicrobial agents and developed the human infection. Current strategies are removed or prevent the microbial colonies from the medical devices, which are attached to the surfaces. This will improve the clinical outcomes in favor of the patients suffering from serious infectious diseases. Moreover, the identification and inhibition of genes, which have the major role in biofilm formation, could be the effective approach for health care systems...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28553415/use-of-proviral-dna-to-investigate-virus-resistance-mutations-in-hiv-infected-zimbabweans
#14
Tutsirai V Musingwini, Danai T Zhou, Doreen Mhandire, Kerina Duri, Exnevia Gomo, Olav Oktedalen, Benjamin Chimukangara, Tinei Shamu, Sandra Shawarira-Bote, Collet Dandara, Babill Stray-Pedersen
BACKGROUND: Antiretroviral therapy (ART) to suppress HIV replication has reduced morbidity and mortality yet effectiveness of current HIV drugs is threatened by HIV drug resistance (HIVDR) mutations. OBJECTIVE: To determine HIVDR mutations using proviral DNA from specimens of patients presenting to an HIV treatment clinic. METHODS: DNA from 103 patients, 86 treatment-experienced, 17 treatment-naïve, were genotyped for the HIV-1C reverse transcriptase gene (RT; codons 21-304) using Sanger sequencing and sequences analyzed using Sequencher software...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28553414/esbl-production-among-e-coli-and-klebsiella-spp-causing-urinary-tract-infection-a-hospital-based-study
#15
Pooja Shakya, Dhiraj Shrestha, Elina Maharjan, Vijay K Sharma, Rabin Paudyal
INTRODUCTION: Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing E. coli and Klebsiella spp. at a tertiary hospital in Nepal. METHODS: A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28217194/prevalence-antibiogram-and-molecular-characterization-of-comunity-acquired-methicillin-resistant-staphylococcus-aureus-in-awka-anambra-nigeria
#16
Blessing Ike, Malachy C Ugwu, Moses N Ikegbunam, David Nwobodo, Chika Ejikeugwu, Thaddeus Gugu, Charles O Esimone
OBJECTIVES: This study evaluated the prevalence, antibiogram and molecular features of CA-MRSA in Awka, Nigeria. METHODS: Confirmation of MRSA was done by testing resistance to oxacillin (1µg), cloxacillin (5µg) and cefoxitin (30µg) on sterile Mueller Hinton agar supplemented with 4% sodium chloride. The MRSA strains were subjected to antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method. Minimum inhibitory concentration was determined using agar dilution method...
2016: Open Microbiology Journal
https://www.readbyqxmd.com/read/28217193/letter-to-the-editor-coinfection-of-adenovirus-with-the-members-of-herpesviridae-in-ophthalmic-pterygium
#17
Mishar Kelishadi, Mandana Kelishadi, Alijan Tabarraei
No abstract text is available yet for this article.
2016: Open Microbiology Journal
https://www.readbyqxmd.com/read/28217192/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-as-a-reliable-tool-to-identify-species-of-catalase-negative-gram-positive-cocci-not-belonging-to-the-streptococcus-genus
#18
Marisa Almuzara, Claudia Barberis, Viviana Rojas Velázquez, Maria Soledad Ramirez, Angela Famiglietti, Carlos Vay
OBJECTIVE: To evaluate the performance of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) by using 190 Catalase-negative Gram-Positive Cocci (GPC) clinical isolates. METHODS: All isolates were identified by conventional phenotypic tests following the proposed scheme by Ruoff and Christensen and MALDI-TOF MS (Bruker Daltonics, BD, Bremen, Germany). Two different extraction methods (direct transfer formic acid method on spot and ethanol formic acid extraction method) and different cut-offs for genus/specie level identification were used...
2016: Open Microbiology Journal
https://www.readbyqxmd.com/read/28077976/antifungal-activity-and-aflatoxin-degradation-of-bifidobacterium-bifidum-and-lactobacillus-fermentum-against-toxigenic-aspergillus-parasiticus
#19
Roshanak Daie Ghazvini, Ebrahim Kouhsari, Ensieh Zibafar, Seyed Jamal Hashemi, Abolfazl Amini, Farhad Niknejad
Food and feedstuff contamination with aflatoxins (AFTs) is a serious health problem for humans and animals, especially in developing countries. The present study evaluated antifungal activities of two lactic acid bacteria (LAB) against growth and aflatoxin production of toxigenic Aspergillus parasiticus. The mycelial growth inhibition rate of A. parasiticus PTCC 5286 was investigated in the presence of Bifidobacterium bifidum PTCC 1644 and Lactobacillus fermentum PTCC 1744 by the pour plate method. After seven days incubation in yeast extract sucrose broth at 30°C, the mycelial mass was weighed after drying...
2016: Open Microbiology Journal
https://www.readbyqxmd.com/read/28077975/evaluate-the-relationship-between-class-1-integrons-and-drug-resistance-genes-in-clinical-isolates-of-pseudomonas-aeruginosa
#20
Seyed Mohammad Javad Hosseini, Niloofar Shoaee Naeini, Azad Khaledi, Seyede Fatemeh Daymad, Davoud Esmaeili
BACKGROUND: The prevalence of resistant Pseudomonas aeruginosa isolates is increasing and it is considered as one of the major public health concerns in the world. The association between integrons and drug resistance has been proven and evidences suggest that integrons are coding and responsible for dissemination of antibiotic resistance among P. aeruginosa isolates. OBJECTIVE: This study is aimed to evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates of P...
2016: Open Microbiology Journal
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