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Open Microbiology Journal

Joshua N Edokpayi, John O Odiyo, Elizabeth O Popoola, Titus A M Msagati
Background: Access to clean and safe drinking water is still a problem in developing countries and more pronounced in rural areas. Due to erratic supply of potable, rural dwellers often seek for an alternative source of water to meet their basic water needs. The objective of this study is to monitor the microbiological and physicochemical water quality parameters of Nzhelele River which is a major alternative source of drinking water to villages along its course in Limpopo province of South Africa...
2018: Open Microbiology Journal
Prakash Shrestha, Hemanta Khanal, Prasanna Dahal, Pranita Dongol
Background: In Nepal, introduction of GeneXpert MTB/RIF assay (Xpert assay) as an initial confirmation test for tuberculosis (TB) has been considered to have impact as a significant decrease in number of clinically diagnosed pulmonary tuberculosis (PTB) cases than previous years. This study aims to find out the distribution profile of suspected tuberculosis cases according to patients age, gender, treatment history and HIV status as well as to evaluate the utility of the Xpert assay over conventional acid-fast bacilli (AFB) staining method for the proper diagnosis of M...
2018: Open Microbiology Journal
Thodoris D Karapantsios
No abstract text is available yet for this article.
2018: Open Microbiology Journal
Yacoub R Nairoukh, Azmi M Mahafzah, Amal Irshaid, Asem A Shehabi
Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method...
2018: Open Microbiology Journal
Mubarak Alfaresi, Bassam Mahboub
Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient...
2017: Open Microbiology Journal
Nehaya Al-Karablieh
Intorduction: Dead Sea is a hypersaline lake with 34% salinity, gains its name due to the absence of any living macroscopic creatures. Despite the extreme hypersaline environment, it is a unique ecosystem for various halophilic microorganisms adapted to this environment. Aims & Objectives: Halophilic microorganisms are known for various potential biotechnological applications, the purpose of the current research is isolation and screening of halophilic bacteria from Dead Sea mud for potential antimicrobial applications...
2017: Open Microbiology Journal
Gabriella Piatti, Marco Bruzzone, Vincenzo Fontana, Alessandro Mannini, Marcello Ceppi
Background: Clostridium Difficile infection (CDI) is considered a ward-based nosocomial infection, due to contagion among patients. Molecular studies recently questioned ward-based contact for disease spread. Objective: To investigate whether it is plausible that CDI spread in San Martino Hospital of Genoa was due to a ward-based contact and patient-to-patient diffusion. Methods: We conducted a retrospective cohort study of CDI cases from April 2010 to March 2015...
2017: Open Microbiology Journal
Gemechu Ameya, Aseer Manilal, Behailu Merdekios
Background: Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria...
2017: Open Microbiology Journal
John F Antiabong, Marleen M Kock, Tsidiso G Maphanga, Adeola M Salawu, Nontombi M Mbelle, Marthie M Ehlers
Background: This study sought to understand the epidemio-ecological dynamics of MRSA isolates associated with a South African hospital over a period spanning year 2007-8 (a previous study reported in 2009) and year 2010-11 (this study). Methods: One hundred and ninety three isolates were characterised by molecular fingerprinting methods including pulsed field gel electrophoresis (PFGE), spa typing, agr -typing, SCC mec -typing, and multilocus sequence typing (MLST)...
2017: Open Microbiology Journal
Reza Ranjbar, Payam Behzadi, Ali Najafi, Raheleh Roudi
Background: A rapid, accurate, flexible and reliable diagnostic method may significantly decrease the costs of diagnosis and treatment. Designing an appropriate microarray chip reduces noises and probable biases in the final result. Objective: The aim of this study was to design and construct a DNA Microarray Chip for a rapid detection and identification of 10 important bacterial agents. Method: In the present survey, 10 unique genomic regions relating to 10 pathogenic bacterial agents including Escherichia coli (E...
2017: Open Microbiology Journal
Irina Kiseleva, Natalie Larionova, Larisa Rudenko
Background: The H5N1 avian influenza was first recognized in humans in Hong Kong 20 years ago. Current enzootic spread of highly pathogenic H5N1 virus among wild and domestic poultry and a number of severe human respiratory diseases caused by this pathogen have stimulated necessity of development of potentially pandemic influenza vaccines. Discussion: In the past few years, significant research was conducted on how to prevent H5N1 influenza. Live, attenuated cold-adapted reassortant influenza vaccine (LAIV) is considered as one of the most promising candidates for pandemic and prepandemic vaccines...
2017: Open Microbiology Journal
John M Thomas, Philip E Thorpe
Objective: Host derived markers on virally infected cells or virions may provide targets for the generation of antiviral agents. Recently, we identified phosphatidylserine (PS) as a host marker of virions and virally-infected cells. Methods and Materials: Under normal physiological conditions, PS is maintained on the inner leaflet of the plasma membrane facing the cytosol. Following viral infection, activation or pre-apoptotic changes cause PS to become externalized...
2017: Open Microbiology Journal
Angus N Oli, Vivian B Akabueze, Chijioke E Ezeudu, George U Eleje, Obiora S Ejiofor, Ifeanyichukwu U Ezebialu, Charlotte B Oguejiofor, Ifeoma M Ekejindu, George O Emechebe, Kenneth N Okeke
Background: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. Objective: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria...
2017: Open Microbiology Journal
Emanuela Lima Dos Santos, Francineyde Alves da Silva, Fábio Sérgio Barbosa da Silva
Background: Libidibia ferrea is a species particular to the caatinga presenting medicinal properties for containing bioactive compounds. The use of Arbuscular Mycorrhizal Fungi (AMF) can increase the production of biomolecules in the legume leaves; however, no light has been shed on the role of symbiosis in maximizing metabolites production in the bark of L. ferrea stem. Objective: The aim was to select AMF that are efficient at increasing the production of phenolic compounds with medicinal properties in the bark of the L...
2017: Open Microbiology Journal
Rinaldo Poncio Mendes, Ricardo de Souza Cavalcante, Sílvio Alencar Marques, Mariângela Esther Alencar Marques, James Venturini, Tatiane Fernanda Sylvestre, Anamaria Mello Miranda Paniago, Ana Carla Pereira, Julhiany de Fátima da Silva, Alexandre Todorovic Fabro, Sandra de Moraes Gimenes Bosco, Eduardo Bagagli, Rosane Christine Hahn, Adriele Dandara Levorato
Background: This review article summarizes and updates the knowledge on paracoccidioidomycosis. P lutzii and the cryptic species of P. brasiliensis and their geographical distribution in Latin America, explaining the difficulties observed in the serological diagnosis. Objectives: Emphasis has been placed on some genetic factors as predisposing condition for paracoccidioidomycosis. Veterinary aspects were focused, showing the wide distribution of infection among animals...
2017: Open Microbiology Journal
Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani
Aims & Objectives: The aim of this study is to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of S. aureus isolated from air, surfaces, staff, and patients in a Public hospital in Ilam. Methods & Materials: A total of 88 of 140 staphylococci identified as S. aureus by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air...
2017: Open Microbiology Journal
Reza Ranjbar, Mehrdad Sami
Background: Antimicrobial resistance is an important factor threatening human health. It is widely accepted that antibiotic resistant bacteria such as Escherichia coli ( E. coli) released from humans and animals into the water sources, can introduce their resistance genes into the natural bacterial community. Objective: The aim of this study was to investigate the prevalence of blaTEM , blaCTX , blaSHV , blaOXA and blaVEB associated-antibiotic resistance among E...
2017: Open Microbiology Journal
Abdulaziz Zorgani, Hiyam Daw, Najib Sufya, Abdullah Bashein, Omar Elahmer, Chedly Chouchani
Introduction: Extended-spectrum β-lactamases (ESBLs), including the AmpC type, are important mechanisms of resistance among Klebsiella pneumoniae and Escherichia coli isolates. Objective: The aim of the study was to investigate the occurrence of AmpC-type β-lactamase producers isolated from two hospitals in Tripoli, Libya. Methods: All clinical isolates (76 K. pneumoniae and 75 E. coli ) collected over two years (2013-2014) were evaluated for susceptibility to a panel of antimicrobials and were analyzed phenotypically for the ESBL and AmpC phenotype using E-test and ESBL and AmpC screen disc test...
2017: Open Microbiology Journal
Shirin Malehmir, Reza Ranjbar, Naser Harzandi
Introduction: Salmonella is known as one of the most important causes of gastrointestinal disease in the world. Quinolones and fluoroquinolones are used successfully in the treatment of salmonellosis particularly for infections that have become resistant to several antibiotics. But non-susceptible isolates to quinolones have been reported in several countries. The data are limited about the prevalence of quinolone-resistant isolates in our country. Therefore, this study investigated the plasmid-mediated quinolone resistance genes in Salmonella enterica isolated in Children's Medical Center in Tehran during 2014-2015...
2017: Open Microbiology Journal
Mardonny Bruno de Oliveira Chagas, Irailton Prazeres Dos Santos, Luis Claudio Nascimento da Silva, Maria Tereza Dos Santos Correia, Janete Magali de Araújo, Marilene da Silva Cavalcanti, Vera Lucia de Menezes Lima
Introduction: In this study, we evaluated the antimicrobial potential of cultivable endophytic fungi associated with Hancornia speciosa Gomes stem bark. Methods and Materials: Plant samples were collected in rainy (July 2010) and dry (January 2011) seasons. In total, 116 endophytic fungi strains were isolated from 90 fragments (64.4% frequency of colonization). Higher fungi frequency was observed in the rainy season (84.4%). The strains were grouped into 14 species; the most frequent were Phoma cava (13...
2017: Open Microbiology Journal
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