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Metallomics: Integrated Biometal Science

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https://www.readbyqxmd.com/read/27918051/detection-of-zn-2-release-in-nitric-oxide-treated-cells-and-proteome-dependence-on-fluorescent-sensor-and-proteomic-sulfhydryl-groups
#1
Mohammad R Karim, David H Petering
Nitric oxide (NO) is both an important regulatory molecule in biological systems and a toxic xenobiotic. Its oxidation products react with sulfhydryl groups and either nitrosylate or oxidize them. The aerobic reaction of NO supplied by diethylamine NONOate (DEA-NO) with pig kidney LLC-PK1 cells and Zn-proteins within the isolated proteome was examined with three fluorescent zinc sensors, zinquin (ZQ), TSQ, and FluoZin-3 (FZ-3). Observations of Zn(2+) labilization from Zn-proteins depended on the specific sensor used...
December 5, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27909710/intracellular-distribution-and-stability-of-a-luminescent-rhenium-i-tricarbonyl-tetrazolato-complex-using-epifluorescence-microscopy-in-conjunction-with-x-ray-fluorescence-imaging
#2
J L Wedding, H H Harris, C A Bader, S E Plush, R Mak, M Massi, D A Brooks, B Lai, S Vogt, M V Werrett, P V Simpson, B W Skelton, S Stagni
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques...
December 2, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27905614/methanobactins-from-genome-to-function
#3
Laura M K Dassama, Grace E Kenney, Amy C Rosenzweig
Methanobactins (Mbns) are ribosomally produced, post-translationally modified peptide (RiPP) natural products that bind copper with high affinity using nitrogen-containing heterocycles and thioamide groups. In some methanotrophic bacteria, Mbns are secreted under conditions of copper starvation and then re-internalized as a copper source for the enzyme particulate methane monooxygenase (pMMO). Genome mining studies have led to the identification and classification of operons encoding the Mbn precursor peptide (MbnA) as well as a number of putative transport, regulatory, and biosynthetic proteins...
December 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27905613/unambiguous-identification-and-discovery-of-bacterial-siderophores-by-direct-injection-21-tesla-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#4
Lawrence R Walker, Malak M Tfaily, Jared B Shaw, Nancy J Hess, Ljiljana Paša-Tolić, David W Koppenaal
Under iron-limiting conditions, bacteria produce low molecular mass Fe(iii) binding molecules known as siderophores to sequester the Fe(iii), along with other elements, increasing their bioavailability. Siderophores are thought to influence iron cycling and biogeochemistry in both marine and terrestrial ecosystems and hence the need for rapid, confident characterization of these compounds has increased. In this study, the type of siderophores produced by two marine bacterial species, Synechococcus sp. PCC 7002 and Vibrio cyclitrophicus 1F53, were characterized by use of a newly developed 21 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICR MS) with direct injection electrospray ionization...
December 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27896349/silver-nanoparticles-a-mechanism-of-action-on-moulds
#5
Katarzyna Pietrzak, Sława Glińska, Magdalena Gapińska, Tomasz Ruman, Adriana Nowak, Egemen Aydin, Beata Gutarowska
Silver nanoparticles (AgNPs) are widely used in all branches of industry. However, their mechanisms of action towards moulds have not been studied yet. Thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (LDI-ToF-MS) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. The AgNP treatment caused the downregulation of 162 (15 ppm) and 284 (62 ppm), and 19 (15 ppm) and 29 (62 ppm) metabolites of Aspergillus niger and Penicillium chrysogenum, respectively...
November 29, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27878189/mapping-cellular-fe-s-cluster-uptake-and-exchange-reactions-divergent-pathways-for-iron-sulfur-cluster-delivery-to-human-ferredoxins
#6
Insiya Fidai, Christine Wachnowsky, J A Cowan
Ferredoxins are protein mediators of biological electron-transfer reactions and typically contain either [2Fe-2S] or [4Fe-4S] clusters. Two ferredoxin homologues have been identified in the human genome, Fdx1 and Fdx2, that share 43% identity and 69% similarity in protein sequence and both bind [2Fe-2S] clusters. Despite the high similarity, the two ferredoxins play very specific roles in distinct physiological pathways and cannot replace each other in function. Both eukaryotic and prokaryotic ferredoxins and homologues have been reported to receive their Fe-S cluster from scaffold/delivery proteins such as IscU, Isa, glutaredoxins, and Nfu...
November 23, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27868128/the-role-of-stim1-in-the-cr-vi-induced-ca-2-i-increase-and-cell-injury-in-l-02-hepatocytes
#7
Xing Yi, Yujing Zhang, Caigao Zhong, Xiali Zhong, Fang Xiao
Hexavalent chromium [Cr(vi)] is a potent cytotoxin and carcinogen. In recent years, drinking water contamination with Cr(vi) has become a worldwide problem of significant public health importance, thus much attention has been paid to the investigation of Cr(vi)-induced hepatotoxicity. The concentration of intracellular calcium ions ([Ca(2+)]i) was found to be increased after Cr(vi) exposure, but the exact underlying mechanisms involved in the Ca(2+) homeostasis imbalance remain poorly characterized. In the present study, by utilizing the antagonist of store-operated calcium channels (SOCCs) 2-aminoethoxydiphenyl borate (2-APB), small interfering RNA against stromal interaction molecule 1 (si-STIM1) and antioxidant N-acetylcysteine (NAC), we found that Cr(vi) induces [Ca(2+)]i increase, cell viability loss and transaminase (AST/ALT) leakage, and that these could be suppressed by both 2-APB and si-STIM1...
November 21, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27853789/chemical-and-functional-properties-of-metal-chelators-that-mobilize-copper-to-elicit-fungal-killing-of-cryptococcus-neoformans
#8
Marian E Helsel, Elizabeth J White, Sayyeda Zeenat A Razvi, Bruno Alies, Katherine J Franz
A panel of iron (Fe) and copper (Cu) chelators was screened for growth inhibitory activity against the fungal pathogen Cryptococcus neoformans. Select bidentate metal-binding ligands containing mixed O,S or O,N donor atoms were identified as agents that induce cell killing in a Cu-dependent manner. Conversely, structurally similar ligands with O,O donor atoms did not inhibit C. neoformans growth regardless of Cu status. Studies of Cu(ii) and Cu(i) binding affinity, lipophilicity, and growth recovery assays of Cu-import deficient cells identified lipophilicity of thermodynamically stable Cu(II)L2 complexes as the best predictor of antifungal activity...
November 17, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27844077/fe-oxy-adducts-of-heme-a%C3%AE-and-heme-hiapp-complexes-intermediates-in-ros-generation
#9
Manas Seal, Soumya Mukherjee, Somdatta Ghosh Dey
Iron (Fe) is the most abundant transition metal ion in the human body and its role, in the form of heme, has been implicated in Alzheimer's disease (AD) and type 2 diabetes mellitus (T2Dm). Heme binds both amyloid beta (Aβ) and human islet amyloid polypeptide (hIAPP) to form heme-Aβ and heme-hIAPP complexes, respectively, and form reactive oxygen species (ROS) like H2O2, O2˙(-)etc., which are known to cause oxidative damage. However the intermediates involved during ROS formation have not yet been isolated...
November 15, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27841435/autophagy-and-apoptosis-studies-on-the-effects-of-bisthiosemicarbazone-copper-ii-complexes-on-p53-and-p53-null-tumour-cell-lines
#10
Franco Bisceglie, Rossella Alinovi, Silvana Pinelli, Maricla Galetti, Marianna Pioli, Pieralberto Tarasconi, Antonio Mutti, Matteo Goldoni, Giorgio Pelosi
A comparative study between two bisthiosemicarbazones, 2,3-butanedione bis(4,4-dimethyl-3-thiosemicarbazone) and 2,3-butanedione bis(2-methyl-3-thiosemicarbazone), and their copper(ii) complexes is reported. The four compounds have been tested on a leukemia cell line U937 (p53-null) and on an adenocarcinoma cell line A549. The study includes cell viability, cell cycle, morphological changes, assessment of apoptosis, analysis of autophagy, measurement of reactive oxygen species (ROS) and of lipid peroxidation, protein determination, assessment of the expression of p53 and cellular uptake of metal complexes...
November 14, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27830853/integration-of-fluorescence-imaging-with-proteomics-enables-visualization-and-identification-of-metallo-proteomes-in-living-cells
#11
Yau-Tsz Lai, Ya Yang, Ligang Hu, Tianfan Cheng, Yuen-Yan Chang, Mohamad Koohi-Moghadam, Yuchuan Wang, Jiang Xia, Junwen Wang, Hongyan Li, Hongzhe Sun
Metalloproteins account for nearly one-third of proteins in proteomes. To date, the identification of metalloproteins relies mainly on protein purification and the subsequent characterization of bound metals, which often leads to losses of metal ions bound weakly and transiently. Herein, we developed a strategy to visualize and subsequently identify endogenous metalloproteins and metal-binding proteins in living cells via integration of fluorescence imaging with proteomics. We synthesized a "metal-tunable" fluorescent probe (denoted as M(n+)-TRACER) that rapidly enters cells to target proteins with 4-40 fold fluorescence enhancements...
November 10, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27782245/particle-size-independent-induction-of-leucism-in-drosophila-melanogaster-by-silver-nano-vs-micro
#12
Kanchan A Phatak, Pawan K Khanna, Bimalendu B Nath
With the discovery of nanoscience, silver nanoparticles (AgNPs) now appear on the ingredient lists of commercial products along with bulk silver or silver microparticles (AgMPs). In the present study, we have compared the effects of silver in both nano and micro forms to assess whether particle size plays a role in the pigmentation pathway, using Drosophila melanogaster as a model organism. AgNPs were synthesized, characterized and validated. Internalization of the AgNPs and AgMPs was confirmed by atomic absorption spectroscopy...
October 26, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27757465/proteomic-responses-to-gold-iii-toxicity-in-the-bacterium-cupriavidus-metallidurans-ch34
#13
Carla M Zammit, Florian Weiland, Joël Brugger, Benjamin Wade, Lyron Juan Winderbaum, Dietrich H Nies, Gordon Southam, Peter Hoffmann, Frank Reith
The metal-resistant β-proteobacterium Cupriavidus metallidurans drives gold (Au) biomineralisation and the (trans)formation of Au nuggets largely via unknown biochemical processes, ultimately leading to the reductive precipitation of mobile, toxic Au(i/iii)-complexes. In this study proteomic responses of C. metallidurans CH34 to mobile, toxic Au(iii)-chloride are investigated. Cells were grown in the presence of 10 and 50 μM Au(iii)-chloride, 50 μM Cu(ii)-chloride and without additional metals. Differentially expressed proteins were detected by difference gel electrophoresis and identified by liquid chromatography coupled mass spectrometry...
October 19, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27738674/in-vitro-characterization-of-a-novel-isu-homologue-from-drosophila-melanogaster-for-de-novo-fes-cluster-formation
#14
Stephen P Dzul, Agostinho G Rocha, Swati Rawat, Ashoka Kandegedara, April Kusowski, Jayashree Pain, Anjaneyulu Murari, Debkumar Pain, Andrew Dancis, Timothy L Stemmler
FeS-clusters are utilized by numerous proteins within several biological pathways that are essential for life. In eukaryotes, the primary FeS-cluster production pathway is the mitochondrial iron-sulfur cluster (ISC) pathway. In Saccharomyces cerevisiae, de novo FeS-cluster formation is accomplished through coordinated assembly with the substrates iron and sulfur by the scaffold assembly protein "Isu1". Sulfur for cluster assembly is provided by cysteine desulfurase "Nfs1", a protein that works in union with its accessory protein "Isd11"...
October 14, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27714016/correction-zinc-is-required-to-ensure-the-expression-of-flagella-and-the-ability-to-form-biofilms-in-salmonella-enterica-sv-typhimurium
#15
Serena Ammendola, Ylenia D'Amico, Barbara Chirullo, Rosanna Drumo, Domenico Ciavardelli, Paolo Pasquali, Andrea Battistoni
Correction for 'Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d.
October 5, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27714043/investigation-of-uranium-interactions-with-calcium-phosphate-binding-proteins-using-icp-ms-and-ce-icp-ms
#16
Thi-Ngoc Suong Huynh, Claude Vidaud, Agnès Hagège
During long-term exposure, uranium accumulates in bone. Since uranium in U(vi) complexes shares similar coordination properties to calcium, this toxicant is assumed to be exchanged with calcium ions at the surfaces of bone mineral crystals. Recently, two proteins involved in bone turnover, fetuin A and osteopontin, were shown to exhibit a high affinity for U(vi). A common biochemical feature of both fetuin A and osteopontin is their inhibiting role in calcium phosphate precipitation. Therefore it is conceivable that complexation of U(vi) with these proteins may alter their interaction with calcium and/or calcium phosphate...
September 30, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27714031/designing-metallodrugs-with-nuclease-and-protease-activity
#17
Caleb Mawuli Agbale, Marlon Henrique Cardoso, Isaac Kojo Galyuon, Octávio Luiz Franco
The accidental discovery of cisplatin some 50 years ago generated renewed interest in metallopharmaceuticals. Beyond cisplatin, many useful metallodrugs have been synthesized for the diagnosis and treatment of various diseases, but toxicity concerns, and the propensity to induce chemoresistance and secondary cancers make it imperative to search for novel metallodrugs that address these limitations. The Amino Terminal Cu(ii) and Ni(ii) (ATCUN) binding motif has emerged as a suitable template to design catalytic metallodrugs with nuclease and protease activities...
September 30, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27714021/toxicological-aspects-of-soluble-titanium-a-review-of-in-vitro-and-in-vivo-studies
#18
M Golasik, M Herman, W Piekoszewski
Owing to the widespread use of titanium dioxide (TiO2) in various food, household and cosmetic products and in industry, the number of publications concerned with the potential toxic effects and health risks related to this substance in both bulk and nano form is steadily increasing. On the other hand, there is also a third form of titanium, ionic, which is produced by the biodegradation of Ti alloy implants. Thus, the growing population of implant recipients should raise concerns about the impact of soluble Ti on human health...
September 29, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27722608/comparison-of-the-metabolism-of-inorganic-and-organic-selenium-species-between-two-selenium-accumulator-plants-garlic-and-indian-mustard
#19
Yasumitsu Ogra, Yurie Ogihara, Yasumi Anan
The metabolism of selenomethionine (SeMet) in two major selenium (Se) accumulator plants, garlic and Indian mustard, was compared to that of stable isotope labeled selenate. Indian mustard more efficiently transported Se from roots to leaves than garlic. In addition, Indian mustard accumulated larger amounts of Se than garlic. γ-Glutamyl-Se-methylselenocysteine (γ-GluMeSeCys) and Se-methylselenocysteine (MeSeCys) were the common metabolites of selenate and SeMet in garlic and Indian mustard. Indian mustard had a specific metabolic pathway to selenohomolanthionine (SeHLan) from both inorganic and organic Se species...
September 23, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27924338/contents-list
#20
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December 7, 2016: Metallomics: Integrated Biometal Science
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