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Metallomics: Integrated Biometal Science

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https://www.readbyqxmd.com/read/28091665/trace-element-protein-interactions-in-endolymph-from-the-inner-ear-of-fish-implications-for-environmental-reconstructions-using-fish-otolith-chemistry
#1
Oliver R B Thomas, Katherine Ganio, Blaine R Roberts, Stephen E Swearer
Otoliths, the biomineralised hearing "ear stones" from the inner ear of fish, grow throughout the lifespan of an individual, with deposition of alternating calciferous and proteinaceous bands occurring daily. Trace element : calcium ratios within daily increments measured by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) are often used in fisheries science to reconstruct environmental histories. There is, however, considerable uncertainty as to which elements are interacting with either the proteinaceous or calciferous zones of the otolith, and thus their utility as indicators of environmental change...
January 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28091661/biochemical-characterization-of-the-selenoproteome-in-gallus-gallus-via-bioinformatics-analysis-structure-function-relationships-and-interactions-of-binding-molecules
#2
Shi-Yong Zhu, Xue-Nan Li, Xiao-Chen Sun, Jia Lin, Wei Li, Cong Zhang, Jin-Long Li
Knowledge about mammalian selenoproteins is increasing. However, the selenoproteome of birds remains considerably less understood, especially concerning its biochemical characterization, structure-function relationships and the interactions of binding molecules. In this work, the SECIS elements, subcellular localization, protein domains and interactions of binding molecules of the selenoproteome in Gallus gallus were analyzed using bioinformatics tools. We carried out comprehensive analyses of the structure-function relationships and interactions of the binding molecules of selenoproteins, to provide biochemical characterization of the selenoproteome in Gallus gallus...
January 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28091657/zinc-transport-and-the-inhibition-of-the-l-type-calcium-channel-are-two-separable-functions-of-znt-1
#3
Eden Shusterman, Ofer Beharier, Shiri Levy, Raz Zarivach, Yoram Etzion, Craig R Campbell, Il-Ha Lee, Anuwat Dinudom, David I Cook, Asher Peretz, Amos Katz, Daniel Gitler, Arie Moran
Traditionally, proteins are considered to perform a single role, be it as an enzyme, a channel, a transporter or as a structural scaffold. However, recent studies have described moonlighting proteins that perform distinct and independent functions; for example, TRPM7 is both an ion channel and a kinase. ZnT-1 is a member of the Carrier Diffusion Facilitator family that is expressed throughout the phylogenetic tree from bacteria to humans. Since its cloning in 1995, ZnT-1 is considered a major extruder of Zn(2+) based on its capability to protect cells against zinc toxicity...
January 16, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28067393/longitudinal-assessment-of-metal-concentrations-and-copper-isotope-ratios-in-the-g93a-sod1-mouse-model-of-amyotrophic-lateral-sclerosis
#4
T Gabriel Enge, Heath Ecroyd, Dianne F Jolley, Justin J Yerbury, Anthony Dosseto
Amyotrophic lateral sclerosis (ALS) is a motor neuron disease, which involves progressive motor neuron degeneration in the central nervous system (CNS). The G93A SOD1 mouse model simulates one of the most common causes of familial ALS through the overexpression of a mutated form of the human gene encoding copper/zinc superoxide dismutase (SOD1). Transition metals, particularly Cu and Zn, have been shown to behave abnormally in the disease context and have been hypothesized to contribute to and potentially trigger the disease...
January 9, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28067386/iron-and-inflammation-the-gut-reaction
#5
REVIEW
Smriti Verma, Bobby J Cherayil
Anemia is a frequent complication of many inflammatory disorders, including inflammatory bowel disease. Although the pathogenesis of this problem is multifactorial, a key component is the abnormal elevation of the hormone hepcidin, the central regulator of systemic iron homeostasis. Investigations over the last decade have resulted in important insights into the role of hepcidin in iron metabolism and the mechanisms that lead to hepcidin dysregulation in the context of inflammation. These insights provide the foundation for novel strategies to prevent and treat the anemia associated with inflammatory diseases...
January 9, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28054081/imaging-metals-in-caenorhabditis-elegans
#6
REVIEW
M Aschner, C Palinski, M Sperling, U Karst, T Schwerdtle, J Bornhorst
Systemic trafficking and storage of essential metal ions play fundamental roles in living organisms by serving as essential cofactors in various cellular processes. Thereby metal quantification and localization are critical steps in understanding metal homeostasis, and how their dyshomeostasis might contribute to disease etiology and the ensuing pathologies. Furthermore, the amount and distribution of metals in organisms can provide insight into their underlying mechanisms of toxicity and toxicokinetics. While in vivo studies on metal imaging in mammalian experimental animals are complex, time- and resource-consuming, the nematode Caenorhabditis elegans (C...
January 5, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28009916/selenium-in-the-prevention-of-atherosclerosis-and-its-underlying-mechanisms
#7
REVIEW
Hongmei Liu, Huibi Xu, Kaixun Huang
Atherosclerosis and related cardiovascular diseases (CVDs) represent the greatest threats to human health worldwide. Selenium, an essential trace element, is incorporated into selenoproteins that play a crucial role in human health and disease. Although findings from a limited number of randomized trials have been inconsistent and cannot support a protective role of Se supplementation in CVDs, prospective observational studies have generally shown a significant inverse association between selenium or selenoprotein status and CVD risk...
December 23, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28001159/effect-of-cu-ii-on-in-vitro-glycation-of-human-serum-albumin-by-methylglyoxal-a-lc-ms-based-proteomic-approach
#8
Alejandra Sarahi Ramirez Segovia, Kazimierz Wrobel, Francisco Javier Acevedo Aguilar, Alma Rosa Corrales Escobosa, Katarzyna Wrobel
It has been reported that glycation of human serum albumin (HSA) changes its capability for copper binding whereas the increase of free copper might have an impact on protein glycation - a key process in diabetes progression. In this work, proteomic analysis of non-glycated HSA and HSA glycated with methylglyoxal (MGo) in the absence or in the presence of Cu(ii) (0.1; 1.0; 5.0 mg Cu L(-1)) has been undertaken. Trypsin hydrolysates were subjected to capillary HPLC-ESI-QTOF-MS and MS/MS. Raw data were analyzed using two proteomic platforms: MaxQuant () and ProteinScape (Bruker)...
December 21, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27942658/defining-the-human-copper-proteome-and-analysis-of-its-expression-variation-in-cancers
#9
S Blockhuys, E Celauro, C Hildesjö, A Feizi, O Stål, J C Fierro-González, P Wittung-Stafshede
Copper (Cu) is essential for living organisms, and acts as a cofactor in many metabolic enzymes. To avoid the toxicity of free Cu, organisms have specific transport systems that 'chaperone' the metal to targets. Cancer progression is associated with increased cellular Cu concentrations, whereby proliferative immortality, angiogenesis and metastasis are cancer hallmarks with defined requirements for Cu. The aim of this study is to gather all known Cu-binding proteins and reveal their putative involvement in cancers using the available database resources of RNA transcript levels...
December 12, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27934997/lead-pb-exposure-reduces-global-dna-methylation-level-by-non-competitive-inhibition-and-alteration-of-dnmt-expression
#10
Oscar F Sanchez, Jinyoung Lee, Nathaphon Yu King Hing, Seong-Eun Kim, Jennifer L Freeman, Chongli Yuan
Low-dose exposure to lead (Pb) is connected to developmental neurological alterations by inducing molecular changes, such as aberrant gene expression patterns. The attributing molecular mechanism, however, is not well-elucidated. In this study, we revealed epigenetic features and mechanisms that can alter gene expression patterns by identifying changes in DNA methyltransferase (DNMT) activity, expression pattern and DNA methylation level using moelcular studies and a zebrafish animal model. We characterized the effects of Pb on the activities of various DNMTs in vitro and determined the molecular role of Pb in modulating DNMT activity via kinetic experiments...
December 9, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27918051/detection-of-zn-2-release-in-nitric-oxide-treated-cells-and-proteome-dependence-on-fluorescent-sensor-and-proteomic-sulfhydryl-groups
#11
Mohammad R Karim, David H Petering
Nitric oxide (NO) is both an important regulatory molecule in biological systems and a toxic xenobiotic. Its oxidation products react with sulfhydryl groups and either nitrosylate or oxidize them. The aerobic reaction of NO supplied by diethylamine NONOate (DEA-NO) with pig kidney LLC-PK1 cells and Zn-proteins within the isolated proteome was examined with three fluorescent zinc sensors, zinquin (ZQ), TSQ, and FluoZin-3 (FZ-3). Observations of Zn(2+) labilization from Zn-proteins depended on the specific sensor used...
December 5, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27909710/intracellular-distribution-and-stability-of-a-luminescent-rhenium-i-tricarbonyl-tetrazolato-complex-using-epifluorescence-microscopy-in-conjunction-with-x-ray-fluorescence-imaging
#12
J L Wedding, H H Harris, C A Bader, S E Plush, R Mak, M Massi, D A Brooks, B Lai, S Vogt, M V Werrett, P V Simpson, B W Skelton, S Stagni
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques...
December 2, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27905614/methanobactins-from-genome-to-function
#13
Laura M K Dassama, Grace E Kenney, Amy C Rosenzweig
Methanobactins (Mbns) are ribosomally produced, post-translationally modified peptide (RiPP) natural products that bind copper with high affinity using nitrogen-containing heterocycles and thioamide groups. In some methanotrophic bacteria, Mbns are secreted under conditions of copper starvation and then re-internalized as a copper source for the enzyme particulate methane monooxygenase (pMMO). Genome mining studies have led to the identification and classification of operons encoding the Mbn precursor peptide (MbnA) as well as a number of putative transport, regulatory, and biosynthetic proteins...
December 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27905613/unambiguous-identification-and-discovery-of-bacterial-siderophores-by-direct-injection-21-tesla-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#14
Lawrence R Walker, Malak M Tfaily, Jared B Shaw, Nancy J Hess, Ljiljana Paša-Tolić, David W Koppenaal
Under iron-limiting conditions, bacteria produce low molecular mass Fe(iii) binding molecules known as siderophores to sequester the Fe(iii), along with other elements, increasing their bioavailability. Siderophores are thought to influence iron cycling and biogeochemistry in both marine and terrestrial ecosystems and hence the need for rapid, confident characterization of these compounds has increased. In this study, the type of siderophores produced by two marine bacterial species, Synechococcus sp. PCC 7002 and Vibrio cyclitrophicus 1F53, were characterized by use of a newly developed 21 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICR MS) with direct injection electrospray ionization...
December 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27853789/chemical-and-functional-properties-of-metal-chelators-that-mobilize-copper-to-elicit-fungal-killing-of-cryptococcus-neoformans
#15
Marian E Helsel, Elizabeth J White, Sayyeda Zeenat A Razvi, Bruno Alies, Katherine J Franz
A panel of iron (Fe) and copper (Cu) chelators was screened for growth inhibitory activity against the fungal pathogen Cryptococcus neoformans. Select bidentate metal-binding ligands containing mixed O,S or O,N donor atoms were identified as agents that induce cell killing in a Cu-dependent manner. Conversely, structurally similar ligands with O,O donor atoms did not inhibit C. neoformans growth regardless of Cu status. Studies of Cu(ii) and Cu(i) binding affinity, lipophilicity, and growth recovery assays of Cu-import deficient cells identified lipophilicity of thermodynamically stable Cu(II)L2 complexes as the best predictor of antifungal activity...
November 17, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27830853/integration-of-fluorescence-imaging-with-proteomics-enables-visualization-and-identification-of-metallo-proteomes-in-living-cells
#16
Yau-Tsz Lai, Ya Yang, Ligang Hu, Tianfan Cheng, Yuen-Yan Chang, Mohamad Koohi-Moghadam, Yuchuan Wang, Jiang Xia, Junwen Wang, Hongyan Li, Hongzhe Sun
Metalloproteins account for nearly one-third of proteins in proteomes. To date, the identification of metalloproteins relies mainly on protein purification and the subsequent characterization of bound metals, which often leads to losses of metal ions bound weakly and transiently. Herein, we developed a strategy to visualize and subsequently identify endogenous metalloproteins and metal-binding proteins in living cells via integration of fluorescence imaging with proteomics. We synthesized a "metal-tunable" fluorescent probe (denoted as M(n+)-TRACER) that rapidly enters cells to target proteins with 4-40 fold fluorescence enhancements...
November 10, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27924338/contents-list
#17
(no author information available yet)
No abstract text is available yet for this article.
December 7, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27924332/back-cover
#18
(no author information available yet)
No abstract text is available yet for this article.
December 7, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27924324/front-cover
#19
(no author information available yet)
No abstract text is available yet for this article.
December 7, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27896349/silver-nanoparticles-a-mechanism-of-action-on-moulds
#20
Katarzyna Pietrzak, Sława Glińska, Magdalena Gapińska, Tomasz Ruman, Adriana Nowak, Egemen Aydin, Beata Gutarowska
Silver nanoparticles (AgNPs) are widely used in all branches of industry. However, their mechanisms of action towards moulds have not been studied yet. Thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (LDI-ToF-MS) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. The AgNP treatment caused the downregulation of 162 (15 ppm) and 284 (62 ppm), and 19 (15 ppm) and 29 (62 ppm) metabolites of Aspergillus niger and Penicillium chrysogenum, respectively...
December 7, 2016: Metallomics: Integrated Biometal Science
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