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Metallomics: Integrated Biometal Science

Anna Espart, Sebastián Artime, Glòria Tort-Nasarre, Edinson Yara-Varón
Cadmium (Cd) is a non-physiological heavy metal that can be harmful at low concentrations. Increasing anthropogenic activities are incrementing the risk of accumulation of this heavy metal in different organs and tissues of the body. In the case of pregnant women, the threat is more serious due to the implications affecting not only their own health but also fetal development as well. Metallothioneins (MTs), small cysteine-rich proteins, are involved in zinc (Zn) and copper homeostasis in mammals but can, however, also bind with Cd if present...
September 17, 2018: Metallomics: Integrated Biometal Science
Tasuku Hirayama, Ayaji Miki, Hideko Nagasawa
Ferroptosis is an emerging type of cell death mode that is dependent on iron. Unfortunately, the detailed analysis of the function of organelle labile Fe(ii) in oxidative damage and lethality of the cells has not been demonstrated so far, mainly due to the lack of efficient methods to visualize labile Fe(ii) at the targeted organelles. We have recently reported a series of Fe(ii)-selective fluorescent probes, i.e., Ac-MtFluNox, Lyso-RhoNox, and ER-SiRhoNox, which can detect Fe(ii) specifically in the mitochondria, lysosomes, and endoplasmic reticulum (ER), respectively...
September 14, 2018: Metallomics: Integrated Biometal Science
Yujing Zhang, Fang Xiao, Caigao Zhong, Ming Zeng, Lianhong Zou
Previous research has shown that cadmium (Cd), which accumulates primarily in the liver and kidneys, could cause cell cycle arrest in various cell types. In the present study, the effect of Cd on the cell cycle progression of hepatocytes and the underlying mechanisms were investigated. Our results showed that Cd inhibited the growth and proliferation of L02 hepatocytes. Further study revealed that Cd significantly induced G2-phase cell cycle arrest accompanied by an overproduction of reactive oxygen species (ROS)...
September 13, 2018: Metallomics: Integrated Biometal Science
Changjun Fan, Guangliang Liu, Yanmin Long, Barry Rosen, Yong Cai
In recent years, methylated thioarsenicals have been widely detected in various biological and environmental matrices, suggesting their broad involvement and biological importance in arsenic metabolism. However, very little is known about the formation mechanism of methylated thioarsenicals and the relation between arsenic methylation and thiolation processes. It is timely and necessary to summarize and synthesize the reported information on thiolated arsenicals for an improved understanding of arsenic thiolation...
September 12, 2018: Metallomics: Integrated Biometal Science
Takaaki Aratake, Youichirou Higashi, Yusuke Ueba, Tomoya Hamada, Takahiro Shimizu, Shogo Shimizu, Toshio Yawata, Tetsuya Ueba, Motoaki Saito
Microglia, the resident immune cells of the central nervous system, can display a pro-inflammatory M1 phenotype or an anti-inflammatory M2 phenotype. Arginase (Arg)-1 expressed in interleukin-4 (IL-4)-induced M2 microglia reduces nitric oxide (NO) production by competing with inducible NO synthase for l-arginine, which contributes to the attenuation of brain inflammation. Although previous studies have indicated that brain zinc promotes M1 activation, the effect of zinc on M2 microglial activation remains to be determined...
September 12, 2018: Metallomics: Integrated Biometal Science
Jianfa Wang, Zhe Liu, Xianjing He, Shuai Lian, Jianbin Liang, Debin Yu, Dongbo Sun, Rui Wu
Selenium (Se) is an important nutritional trace element possessing antioxidant properties. Our goal was to elucidate the effect and mechanism of Se deficiency on the intestinal cell fate. One-day-old three-yellow chickens were fed a low Se diet for 1, 3, and 5 weeks. Histologic characteristics, protein expression profiles, antioxidant activities, inflammatory signaling, and the apoptosis status in duodenum mucosa were investigated. Histological results showed that Se deficiency could increase inflammatory cell infiltration, karyopyknosis of the epithelial cells, cytoplasm vacuolization and dissolution of goblet cells...
September 12, 2018: Metallomics: Integrated Biometal Science
Angelique N Besold, Edward M Culbertson, Lily Nam, Ryan P Hobbs, Alisa Boyko, C Noel Maxwell, Walter J Chazin, Adriana R Marques, Valeria C Culotta
Calprotectin is a potent antimicrobial that inhibits the growth of pathogens by tightly binding transition metals such as Mn and Zn, thereby preventing their uptake and utilization by invading microbes. At sites of infection, calprotectin is abundantly released from neutrophils, but calprotectin is also present in non-neutrophil cell types that may be relevant to infections. We show here that in patients infected with the Lyme disease pathogen Borreliella (Borrelia) burgdorferi, calprotectin is produced in neutrophil-free regions of the skin, in both epidermal keratinocytes and in immune cells infiltrating the dermis, including CD68 positive macrophages...
September 12, 2018: Metallomics: Integrated Biometal Science
Rima Roy, Saikat Samanta, Surajit Patra, Nav Kumar Mahato, Rudra P Saha
The ArsR-SmtB family of proteins displays the greatest diversity among the bacterial metal-binding transcriptional regulators with regard to the variety of metal ions that they can sense. In the presence of increased levels of toxic heavy metals, these proteins dissociate from their cognate DNA upon the direct binding of metal ions to the appropriate sites, designated motifs on the proteins, either at the interface of the dimers or at the intra-subunit locations. In addition to the metal-mediated regulation, some proteins were also found to control transcription via redox reactions...
September 7, 2018: Metallomics: Integrated Biometal Science
Jelena Habjanič, Oliver Zerbe, Eva Freisinger
Metallothioneins (MTs) are crucial players in metal-related physiological processes. They are characterized by a high cysteine content and unique metal binding properties resulting in specific metal-thiolate clusters formation. Here we present the first NMR solution structure of a Pseudomonas MT, PflQ2 MT, using the strain P. fluorescens Q2-87. It consists of a metal binding domain and an intrinsically disordered C-terminal tail, that was not observed in other MTs so far. While not influencing the structure or function of the metal binding domain, the tail contains a potential binding motif that might be important in so far undiscovered biological interactions...
September 7, 2018: Metallomics: Integrated Biometal Science
Paula Pongrac, Tânia S Serra, Hiram Castillo-Michel, Katarina Vogel-Mikuš, Iztok Arčon, Mitja Kelemen, Boštjan Jenčič, Anja Kavčič, Mina T Villafort Carvalho, Mark G M Aarts
Cadmium (Cd) was shown to co-localise with calcium (Ca) in oxalate crystals in the stems and leaves of Cd tolerant Gomphrena claussenii, but Cd binding remained unresolved. Using synchrotron radiation X-ray absorption near edge spectroscopy we demonstrate that in oxalate crystals of hydroponically grown G. claussenii the vast majority of Cd is bound to oxygen ligands in oxalate crystals (>88%; Cd-O-C coordination) and the remaining Cd is bound to sulphur ligands (Cd-S-C coordination). Cadmium binding to oxalate does not depend on the amount of Ca supplied or from which organs the crystals originate (stems and mature leaves)...
September 5, 2018: Metallomics: Integrated Biometal Science
Karam Chand, Rajeshwari, Emanuel Candeias, Sandra M Cardoso, Sílvia Chaves, M Amélia Santos
Alzheimer's disease (AD) is a severe age-dependent neurodegenerative disorder affecting several million people worldwide. So far, there is no adequate medication to prevent or slow down the progression of the disease, only medication with palliative effects allowing temporary symptomatic reliefs. As part of our continuing efforts into the development of innovative drugs following a polypharmacological strategy, we decided to use a former anti-AD palliative drug (tacrine) and to reposition it by hybridization with a metal chelating drug (deferiprone, DFP)...
September 5, 2018: Metallomics: Integrated Biometal Science
Alaa T Al-Sandaqchi, Chris Brignell, Joanna F Collingwood, Kalotina Geraki, Evgeny M Mirkes, Kenny Kong, Marcos Castellanos, Sean T May, Carl W Stevenson, Hany M Elsheikha
In this study, we measured the levels of elements in human brain microvascular endothelial cells (ECs) infected with T. gondii. ECs were infected with tachyzoites of the RH strain, and at 6, 24, and 48 hours post infection (hpi), the intracellular concentrations of elements were determined using a synchrotron-microfocus X-ray fluorescence microscopy (μ-XRF) system. This method enabled the quantification of the concentrations of Zn and Ca in infected and uninfected (control) ECs at sub-micron spatial resolution...
September 5, 2018: Metallomics: Integrated Biometal Science
Agnieszka Mierek-Adamska, Grażyna B Dąbrowska, Claudia A Blindauer
The problem of handling zinc in the cell is of great importance because zinc is an indispensable micronutrient involved in most physiological processes in all living organisms. Moreover, our understanding of mechanisms governing the discrimination between micronutrients and toxic metals on the level of individual proteins to the whole-organism level is incomplete. Metallothioneins are able to bind heavy metal ions, and roles in zinc homeostasis have been proposed. Here, we have studied the in vitro and in vivo metal-binding abilities of Brassica napus type 4 metallothionein (BnMT4) and its expression in germinating seeds in response to metal treatment...
September 3, 2018: Metallomics: Integrated Biometal Science
Mickaël V Cherrier, Patricia Amara, Barisa Talbi, Michèle Salmain, Juan C Fontecilla-Camps
The X-ray structure of an aerated achiral Ru-papain conjugate has revealed the hydroxylation of two tyrosine residues found near the ruthenium ion. The most likely mechanism involves a ruthenium-bound superoxide as the reactive species responsible for the first hydroxylation and the resulting high valent Ru(iv)[double bond, length as m-dash]O species for the second one.
September 3, 2018: Metallomics: Integrated Biometal Science
Belén Callejón-Leblic, José Luis Gómez-Ariza, Antonio Pereira-Vega, Tamara García-Barrera
Lung cancer (LC) is one of the most common causes of cancer-related deaths in the world and it is well known that trace elements play important roles in the carcinogenic process activating and inhibiting enzymatic reactions and metalloproteins, in which they usually participate as cofactors. A cross-sectional study was conducted on 48 lung cancer patients and 39 controls (56 men and 31 women), aged 44-76 years between March 2011 and June 2012. Eleven elements have been included in the study: V, Cr, Mn, Fe, Co, Cu, Zn, Se, Mo, Cd, and Pb, some of them considered toxic (V, Cd, Cr and Pb), while others are essential (Co, Mo, Se, Fe and Zn), and they have been analyzed by ICP-QQQ-MS in serum, urine and for the first time in bronchoalveolar lavage fluid (BALF)...
August 31, 2018: Metallomics: Integrated Biometal Science
Amelia Sedjahtera, Lydia Gunawan, Lisa Bray, Lin Wai Hung, Jack Parsons, Nobuyuki Okamura, Victor L Villemagne, Kazuhiko Yanai, Xiang M Liu, Jacky Chan, Ashley I Bush, David I Finkelstein, Kevin J Barnham, Robert A Cherny, Paul A Adlard
Tauopathies are characterized by the pathological accumulation of the microtubule associated protein tau within the brain. We demonstrate here that a copper/zinc chaperone (PBT2, Prana Biotechnology) has rapid and profound effects in the rTg(tauP301L)4510 mouse model of tauopathy. This was evidenced by significantly improved cognition, a preservation of neurons, a decrease in tau aggregates and a decrease in other forms of "pathological" tau (including phosphorylated tau and sarkosyl-insoluble tau)...
August 31, 2018: Metallomics: Integrated Biometal Science
Tian Xiao, Junchao Xue, Ming Shi, Chao Chen, Fei Luo, Hui Xu, Xiong Chen, Baofei Sun, Qian Sun, Qianlei Yang, Xiangyu Dai, Aihua Zhang, Huanwen Tang, Qizhan Liu
Arsenic is a known human carcinogen and the mechanisms underlying arsenic-induced tumorigenesis remain elusive. Circular RNAs (circRNAs) are involved in the development of cancers, generally acting as sponges for microRNAs (miRNAs). Here, we screened the circRNA expression profiles of HaCaT cells, which are immortalized human keratinocytes, and arsenite-transformed HaCaT cells (T-HaCaT). The presence of has_circRNA-008913 (circ008913) was confirmed in HaCaT cells. Among the circRNAs down-regulated in T-HaCaT cells, circ008913 showed the greatest decrease and was chosen for further research...
September 19, 2018: Metallomics: Integrated Biometal Science
Jian Wang, Song Wang, Pengcheng Sun, Fangqi Cao, Hui Li, Jing Sun, Min Peng, Wenbin Liu, Ping Shi
Lipin1 participates in numerous cellular processes, including in the dephosphorylation of phosphatidic acid to diacylglycerol and as a co-transcriptional regulator. Iron is also essential in various critical biological processes. Previous studies have shown that compared to normal tissue cells, lipin1 expression and iron metabolism are abnormal in cancer cells. However, the involvement of lipin1 in the regulation of iron metabolism is unknown. In this study, we compared the contents of eight metal ions (potassium, calcium, sodium, magnesium, manganese, zinc, iron and copper) in human hepatoma carcinoma BEL7402 control cells as well as stable cells overexpressing lipin1 by using ICP-AES...
August 24, 2018: Metallomics: Integrated Biometal Science
Bilal Ahmed, Mohammad Shahid, Mohammad Saghir Khan, Javed Musarrat
There has been rapid increase globally in the production of functionally divergent nanoparticles in recent times. The uncontrolled discharge of such nanomaterials is a serious threat to the environment. We assess the impact of various-sized metal oxide nanoparticles (MONPs) on cell cycle progression and induction of oxidative stress in onions. Of these, CuO-NPs and TiO2-NPs significantly reduced the mitotic index (MI) by 28% and 17%, respectively, whereas Al2O3-NPs augmented the MI by 13% compared to untreated onion roots...
September 19, 2018: Metallomics: Integrated Biometal Science
Megha Mittal, Sreemoyee Chatterjee, S J S Flora
INTRODUCTION: Arsenic and fluoride are recognized globally as the most serious inorganic contaminants in drinking water. As there is no safe and effective treatment for the cases of fluoride poisoning and combined arsenic-fluoride toxicity, the present study was planned to assess (i) the mechanism of combined exposure to arsenic and fluoride via biochemical and spectroscopic data; (ii) the effect of a thiol chelating agent, meso-2,3-dimercaptosuccinic acid (DMSA), either individually or in combination with the antioxidant vitamin C in reversing arsenic-fluoride toxicity; and (iii) whether combination therapy enhances arsenic and fluoride removal from blood and soft tissues...
September 19, 2018: Metallomics: Integrated Biometal Science
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