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Metallomics: Integrated Biometal Science

Carla M Zammit, Florian Weiland, Joël Brugger, Benjamin Wade, Lyron Juan Winderbaum, Dietrich H Nies, Gordon Southam, Peter Hoffmann, Frank Reith
The metal-resistant β-proteobacterium Cupriavidus metallidurans drives gold (Au) biomineralisation and the (trans)formation of Au nuggets largely via unknown biochemical processes, ultimately leading to the reductive precipitation of mobile, toxic Au(i/iii)-complexes. In this study proteomic responses of C. metallidurans CH34 to mobile, toxic Au(iii)-chloride are investigated. Cells were grown in the presence of 10 and 50 μM Au(iii)-chloride, 50 μM Cu(ii)-chloride and without additional metals. Differentially expressed proteins were detected by difference gel electrophoresis and identified by liquid chromatography coupled mass spectrometry...
October 19, 2016: Metallomics: Integrated Biometal Science
Stephen P Dzul, Agostinho G Rocha, Swati Rawat, Ashoka Kandegedara, April Kusowski, Jayashree Pain, Anjaneyulu Murari, Debkumar Pain, Andrew Dancis, Timothy L Stemmler
FeS-clusters are utilized by numerous proteins within several biological pathways that are essential for life. In eukaryotes, the primary FeS-cluster production pathway is the mitochondrial iron-sulfur cluster (ISC) pathway. In Saccharomyces cerevisiae, de novo FeS-cluster formation is accomplished through coordinated assembly with the substrates iron and sulfur by the scaffold assembly protein "Isu1". Sulfur for cluster assembly is provided by cysteine desulfurase "Nfs1", a protein that works in union with its accessory protein "Isd11"...
October 14, 2016: Metallomics: Integrated Biometal Science
Melani Sooriyaarachchi, Graham N George, Ingrid J Pickering, Aru Narendran, Jürgen Gailer
Numerous in vivo studies have shown that the severe toxic side-effects of intravenously administered cisplatin can be significantly reduced by the co-administration of sulfur-containing 'chemoprotective agents'. Using a metallomics approach, a likely biochemical basis for these potentially useful observations was only recently uncovered and appears to involve the reaction of chemoprotective agents with cisplatin-derived Pt-species in human plasma to form novel platinum-sulfur complexes (PSC's). We here reveal aspects of the structure of two PSC's and establish the identification of an optimal chemoprotective agent to ameliorate the toxic side-effects of cisplatin, while leaving its antineoplastic activity largely intact, as a feasible research strategy to transform cisplatin into a safer and more effective anticancer drug...
October 10, 2016: Metallomics: Integrated Biometal Science
Rafael Barros de Souza, Rayssa Karla Silva, Dayvison Soares Ferreira, Sérgio de Sá Leitão Paiva Junior, Will de Barros Pita, Marcos Antonio de Morais Junior
In a recent work we showed that magnesium (Mg(II)) plays an important role in industrial ethanol production, overcoming the negative effect of the excess of minerals, particularly copper, present in sugarcane juice, with a consequent increase in ethanol yield. This cation has been reported to be involved in several steps of yeast metabolism, acting mainly as a co-factor of several enzymes of fermentation metabolism and protecting yeast cells from stressful conditions. However, despite many physiological investigations, its effect in the molecular mechanisms that control such metabolic activities remains unclear and to date no information concerning its influence on gene expression has been provided...
October 7, 2016: Metallomics: Integrated Biometal Science
Serena Ammendola, Ylenia D'Amico, Barbara Chirullo, Rosanna Drumo, Domenico Ciavardelli, Paolo Pasquali, Andrea Battistoni
Correction for 'Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d.
October 5, 2016: Metallomics: Integrated Biometal Science
Thi-Ngoc Suong Huynh, Claude Vidaud, Agnès Hagège
During long-term exposure, uranium accumulates in bone. Since uranium in U(vi) complexes shares similar coordination properties to calcium, this toxicant is assumed to be exchanged with calcium ions at the surfaces of bone mineral crystals. Recently, two proteins involved in bone turnover, fetuin A and osteopontin, were shown to exhibit a high affinity for U(vi). A common biochemical feature of both fetuin A and osteopontin is their inhibiting role in calcium phosphate precipitation. Therefore it is conceivable that complexation of U(vi) with these proteins may alter their interaction with calcium and/or calcium phosphate...
September 30, 2016: Metallomics: Integrated Biometal Science
Caleb Mawuli Agbale, Marlon Henrique Cardoso, Isaac Kojo Galyuon, Octávio Luiz Franco
The accidental discovery of cisplatin some 50 years ago generated renewed interest in metallopharmaceuticals. Beyond cisplatin, many useful metallodrugs have been synthesized for the diagnosis and treatment of various diseases, but toxicity concerns, and the propensity to induce chemoresistance and secondary cancers make it imperative to search for novel metallodrugs that address these limitations. The Amino Terminal Cu(ii) and Ni(ii) (ATCUN) binding motif has emerged as a suitable template to design catalytic metallodrugs with nuclease and protease activities...
September 30, 2016: Metallomics: Integrated Biometal Science
M Golasik, M Herman, W Piekoszewski
Owing to the widespread use of titanium dioxide (TiO2) in various food, household and cosmetic products and in industry, the number of publications concerned with the potential toxic effects and health risks related to this substance in both bulk and nano form is steadily increasing. On the other hand, there is also a third form of titanium, ionic, which is produced by the biodegradation of Ti alloy implants. Thus, the growing population of implant recipients should raise concerns about the impact of soluble Ti on human health...
September 29, 2016: Metallomics: Integrated Biometal Science
Yasumitsu Ogra, Yurie Ogihara, Yasumi Anan
The metabolism of selenomethionine (SeMet) in two major selenium (Se) accumulator plants, garlic and Indian mustard, was compared to that of stable isotope labeled selenate. Indian mustard more efficiently transported Se from roots to leaves than garlic. In addition, Indian mustard accumulated larger amounts of Se than garlic. γ-Glutamyl-Se-methylselenocysteine (γ-GluMeSeCys) and Se-methylselenocysteine (MeSeCys) were the common metabolites of selenate and SeMet in garlic and Indian mustard. Indian mustard had a specific metabolic pathway to selenohomolanthionine (SeHLan) from both inorganic and organic Se species...
September 23, 2016: Metallomics: Integrated Biometal Science
Victor P Bondanese, Aline Lamboux, Melanie Simon, Jérôme E Lafont, Emmanuelle Albalat, Sylvain Pichat, Jean-Marc Vanacker, Philippe Telouk, Vincent Balter, Philippe Oger, Francis Albarède
Hepatocellular carcinoma (HCC) is the most frequent type of primary liver cancer, with increasing incidence worldwide. The unrestrained proliferation of tumour cells leads to tumour hypoxia which in turn promotes cancer aggressiveness. While changes in the concentration of copper (Cu) have long been observed upon cancerization, we have recently reported that the isotopic composition of copper is also altered in several types of cancer. In particular, we showed that in hepatocellular carcinoma, tumour tissue contains heavier copper compared to the surrounding parenchyma...
August 8, 2016: Metallomics: Integrated Biometal Science
J Alonso García, D Turiel Fernández, E Añón Álvarez, E Blanco González, M Montes-Bayón, A Sanz-Medel
Iron is an essential element for cell growth and division. Recent experiments have linked a deregulation of iron's metabolism with breast cancer progression, aggressiveness and recurrence. In fact, it is conceived that chronic failure in the redox balance due to the presence of a high intracellular concentration of this metal has the potential to modulate specific signaling networks associated with cancer malignancy. Thus, this work has been focused on the comparative evaluation of part of the Fe metallome in two breast cancer cell lines of different malignancies: MCF-7 and MDA-MB-231...
October 1, 2016: Metallomics: Integrated Biometal Science
Serena Ammendola, Ylenia D'Amico, Barbara Chirullo, Rosanna Drumo, Domenico Civardelli, Paolo Pasquali, Andrea Battistoni
Zinc is known to play a central role in bacterial physiology and pathogenesis. Here, we report that the accumulation of FliC, the structural subunit of Salmonella phase 1 flagella, is sharply reduced in a znuABC Salmonella enterica sv. Typhimurium strain grown in zinc-poor media. Consequently, this mutant strain lacks motility, unless it grows in zinc-replete environments. This phenotype is the consequence of a general downregulation of all the genes involved in the biosynthesis of flagella, suggesting that zinc is the cofactor of proteins involved in the initiation of the transcriptional regulatory cascade leading to flagella assembly...
October 1, 2016: Metallomics: Integrated Biometal Science
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October 1, 2016: Metallomics: Integrated Biometal Science
(no author information available yet)
No abstract text is available yet for this article.
October 1, 2016: Metallomics: Integrated Biometal Science
(no author information available yet)
No abstract text is available yet for this article.
October 1, 2016: Metallomics: Integrated Biometal Science
Jiaojiao Li, Shashank S Pawitwar, Barry P Rosen
Arsenic is the most pervasive environmental toxic substance. As a consequence of its ubiquity, nearly every organism has genes for resistance to inorganic arsenic. In bacteria these genes are found largely in bacterial arsenic resistance (ars) operons. Recently a parallel pathway for synthesis and degradation of methylated arsenicals has been identified. The arsM gene product encodes the ArsM (AS3MT in animals) As(iii) S-adenosylmethionine methyltransferase that methylates inorganic trivalent arsenite in three sequential steps to methylarsenite MAs(iii), dimethylarsenite (DMAs(iii) and trimethylarsenite (TMAs(iii))...
October 1, 2016: Metallomics: Integrated Biometal Science
Clémence Cheignon, Peter Faller, Denis Testemale, Christelle Hureau, Fabrice Collin
In the context of Alzheimer's disease (AD), the production of HO˙ by copper-amyloid beta (Aβ) in the presence of ascorbate is known to be deleterious for the Aβ peptide itself and also for the surrounding molecules, thus establishing a direct link between AD and oxidative stress. The metal-catalyzed oxidation (MCO) of Aβ primarily targets the residues involved in copper coordination during HO˙ production. In the present work, we demonstrate that the oxidative damage undergone by Aβ during MCO lead to a change in copper coordination, with enhanced catalytic properties that increases the rates of ascorbate consumption and HO˙ production, and the amount of HO˙ released by the system...
October 1, 2016: Metallomics: Integrated Biometal Science
N Maio, T A Rouault
Iron-sulfur (Fe-S) clusters are inorganic cofactors that are fundamental to several biological processes in all three kingdoms of life. In most organisms, Fe-S clusters are initially assembled on a scaffold protein, ISCU, and subsequently transferred to target proteins or to intermediate carriers by a dedicated chaperone/co-chaperone system. The delivery of assembled Fe-S clusters to recipient proteins is a crucial step in the biogenesis of Fe-S proteins, and, in mammals, it relies on the activity of a multiprotein transfer complex that contains the chaperone HSPA9, the co-chaperone HSC20 and the scaffold ISCU...
October 1, 2016: Metallomics: Integrated Biometal Science
Raúl Mera-Adasme, Hannes Erdmann, Tomasz Bereźniak, Christian Ochsenfeld
Amyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease, with no effective pharmacological treatment. Its pathogenesis is unknown, although a subset of the cases is linked to genetic mutations. A significant fraction of the mutations occur in one protein, copper, zinc superoxide dismutase (SOD1). The toxic function of mutant SOD1 has not been elucidated, but damage to the metal site of the protein is believed to play a major role. In this work, we study the electrostatic loop of SOD1, which we had previously proposed to work as a "solvent seal" isolating the metal site from water molecules...
October 1, 2016: Metallomics: Integrated Biometal Science
Daisy Bourassa, Sophie-Charlotte Gleber, Stefan Vogt, Chong Hyun Shin, Christoph J Fahrni
Transition metals such as zinc, copper, and iron play key roles in cellular proliferation, cell differentiation, growth, and development. Over the past decade, advances in synchrotron X-ray fluorescence instrumentation presented new opportunities for the three-dimensional mapping of trace metal distributions within intact specimens. Taking advantage of microXRF tomography, we visualized the 3D distribution of zinc and iron in a zebrafish embryo at the onset of the hatching period. The reconstructed volumetric data revealed distinct differences in the elemental distributions, with zinc predominantly localized to the yolk and yolk extension, and iron to various regions of the brain as well as the myotome extending along the dorsal side of the embryo...
October 1, 2016: Metallomics: Integrated Biometal Science
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