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Molecular Medicine Reports

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https://www.readbyqxmd.com/read/28393255/surfactant-protein-a-is-expressed-in-the-central-nervous-system-of-rats-with-experimental-autoimmune-encephalomyelitis-and-suppresses-inflammation-in-human-astrocytes-and-microglia
#1
Xue Yang, Jun Yan, Juan Feng
The collectin surfactant protein‑A (SP‑A), a potent host defense molecule, is well recognized for its role in the maintenance of pulmonary homeostasis and the modulation of inflammatory responses. While previous studies have detected SP‑A in numerous extrapulmonary tissues, there is still a lack of information regarding its expression in central nervous system (CNS) and potential effects in neuroinflammatory diseases, such as multiple sclerosis (MS). The present study used experimental autoimmune encephalomyelitis (EAE), the most commonly used animal model of MS, to investigate the expression of SP‑A in the CNS at different stages of disease progression...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393250/inhibition-of-varicella%C3%A2-zoster-virus-replication-by-an-ethanol-extract-of-lysimachia-mauritiana
#2
Sohee Bae, Yoon-Jae Song
Varicella‑zoster virus (VZV), a ubiquitous human α‑herpesvirus, is the causative agent of chickenpox and shingles. In the present study, we investigated the antiviral activity of a 70% ethanol extract of Lysimachia mauritiana (LME) against VZV. LME strongly interfered with the replication of both laboratory and clinical strains of VZV without affecting the viability of MRC‑5 cells. Moreover, LME treatment suppressed the expression of an essential viral transactivator, immediate‑early 62 protein (IE62), in addition to other lytic genes in the later phases of viral replication...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393249/epcam-and-cox%C3%A2-2-expression-are-positively-correlated-in-human-breast-cancer
#3
Shuhang Gao, Yan Sun, Xue Liu, Dandan Zhang, Xuesong Yang
Upregulation of the epithelial cell adhesion molecule (EpCAM) is involved in tumor progression. Cyclooxygenase (COX)‑2 is the key enzyme catalyzing prostaglandin synthesis and is involved in breast cancer progression and metastasis. However, the prognostic value of EpCAM and its putative correlation with COX‑2 in breast cancer have yet to be elucidated. The aim of the present study was to assess the clinical relevance of the relationship between EpCAM and COX‑2, via examining the putative correlation between EpCAM and COX‑2 expression in various types of human breast cancer...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393248/epigallocatechin-3-gallate-ameliorates-insulin-resistance-in-hepatocytes
#4
Shan-Bo Ma, Rui Zhang, Shan Miao, Bin Gao, Yang Lu, Sen Hui, Long Li, Xiao-Peng Shi, Ai-Dong Wen
Hyperglycemia is a typical pathogenic factor in a series of complications among patients with type II diabetes. Epigallocatechin-3-gallate (EGCG) is the major polyphenol extracted from green tea and is reported to be an antioxidant. The aim of the present study was to examine the effect of EGCG on insulin resistance in human HepG2 cells pretreated with high concentrations of glucose. The protein kinase B (AKT)/glycogen synthase kinase (GSK) pathways were analyzed using western blot analysis in HepG2 cells and primary mouse hepatocytes treated with high glucose and/or EGCG...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393247/bioinformatics-analysis-of-gene-expression-alterations-in-microrna%C3%A2-122-knockout-mice-with-hepatocellular-carcinoma
#5
Bosheng He, Ying He, Weixiang Shi, Shenchu Gong, Xiaohong Chen, Jing Xiao, Jinhua Gu, Wenbin Ding, Yilang Wang
Reduced microRNA (miR)‑122 expression levels are frequently observed in hepatocellular carcinoma (HCC). The present study was conducted to investigate potential targets of miR‑122 and determine the underlying regulatory mechanisms of miR‑122 in HCC development. The public dataset GSE31731 was utilized, consisting of 8 miR‑122 knockout (KO) mice (miR‑122 KO) and 8 age‑matched wild‑type mice (WT group). Following data preprocessing, the differentially expressed genes (DEGs) were selected, followed by enrichment analysis...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393245/knockdown-of-fstl1-inhibits-pdgf%C3%A2-bb%C3%A2-induced-human-airway-smooth-muscle-cell-proliferation-and-migration
#6
Yuelin Deng, Yao Zhang, Huajie Wu, Zhaoling Shi, Xin Sun
Abnormal proliferation and migration of airway smooth muscle (ASM) cells serve roles in airway remodeling, and contribute to airway hyper‑responsiveness. Follistatin‑like protein 1 (FSTL1) is a secreted glycoprotein that belongs to the follistatin family of proteins. It was reported that in the lungs of patients suffering from severe asthma, FSTL1 is highly expressed by macrophages. However, the role of FSTL1 in ASM cell proliferation and migration remains unknown. The present study aimed to investigate the role of FSTL1 in cell proliferation and migration mediated by platelet‑derived growth factor subunit B (PDGF‑BB) in human ASM cells...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393240/do-in-seung-gi%C3%A2-tang-extract-suppresses-adipocyte-differentiation-in-3t3%C3%A2-l1-cells
#7
Eun Hyoung Shim, Myeong Soo Lee, Ju-Ah Lee, Hoyoung Lee
Blood stasis syndrome (BSS), additionally called Eohyul, is a basic pathological concept in Traditional Korean Medicine. Do In Seung Gi‑Tang (DISGT) is herbal medicine used for the treatment of BSS. It primarily treats metabolic diseases (MDs) including obesity, hypertension, diabetes mellitus and gynecological diseases, by promoting blood circulation. The present study aimed to investigate the anti‑adipogenesis effect of DISGT in 3T3‑L1 adipocytes using Oil Red O staining, and assessing levels of triglycerides (TGs) and leptin in adipocytes by ELISA and western blot analysis...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393227/neutrophil-gelatinase%C3%A2-associated-lipocalin-as-a-potential-novel-biomarker-for-ventilator%C3%A2-associated-lung-injury
#8
Rui Xiao, Rongchang Chen
Neutrophil gelatinase‑associated lipocalin (NGAL) is a 25‑kDa protein of the lipocalin superfamily and its presence was initially observed in activated neutrophils. It has previously been demonstrated that the expression of NGAL is markedly increased in stimulated epithelia, and is important in the innate immunological response to various pathophysiological conditions, including infection, cancer, inflammation and kidney injury. The present study constructed a ventilator‑associated lung injury model in mice...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393226/removal-from-adherent-culture-contributes-to-apoptosis-in-human-bone-marrow-mesenchymal-stem-cells
#9
Baoping Deng, Haiming Jiang, Kuan Zeng, Yi Liang, Yinmeng Wu, Yanqi Yang
Mesenchymal stem cells (MSCs) are routinely isolated due to their adherence to tissue culture plates and their in vitro growth characteristics. Expansion of MSCs in adherent cultures is the only way to obtain sufficient cells for use in either clinical or research settings. MSCs have tremendous potential in myocardial repair treatment by cell therapy techniques, however, a large number of MSCs die from apoptosis following transplantation. Previous studies have examined the factors contributing to the survival of transplanted cells, but little is known about the effect of removal from adherent culture conditions on apoptosis of the MSCs...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393223/pyropia-yezoensis-peptide-pyp1%C3%A2-5-protects-against-dexamethasone%C3%A2-induced-muscle-atrophy-through-the-downregulation-of-atrogin1-mafbx-and-murf1-in-mouse-c2c12-myotubes
#10
Min-Kyeong Lee, Young-Min Kim, In-Hye Kim, Youn-Hee Choi, Taek-Jeong Nam
Skeletal muscle atrophy refers to the decline in muscle mass and strength that occurs under various conditions, including aging, starvation, cancer and other cachectic diseases. Muscle atrophy caused by aging, known as sarcopenia, primarily occurs after 50 years of age. Muscle atrophy‑related genes, including atrogin1/muscle atrophy F‑box (MAFbx) and muscle RING finger 1 (MuRF1), are expressed early in the muscle atrophy process, and their expression precedes the loss of muscle mass. The present study investigated the potential anti‑atrophic effects of the Pyropia yezoensis peptide PYP1‑5...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393207/rna-interference-mediated-silencing-of-ppgalnac-t1-and-ppgalnac-t2-inhibits-invasion-and-increases-chemosensitivity-potentially-by-reducing-terminal-%C3%AE-2-3-sialylation-and-mmp14-expression-in-triple%C3%A2-negative-breast-cancer-cells
#11
Hao Qiu, Xu Xu, Min Liu, Zerong Wang, Yaqin Yuan, Chunliang Liu, Lan Xu, Shiliang Wu
Glycopeptide-preferring polypeptide N-acetylgalactosamine transferase (ppGalNAc‑T) is a key enzyme that initiates the formation of the first GalNAc monosaccharide to polypeptides at Thr/Ser residues by O‑linked glycosylation. In order to investigate the effects of ppGalNAc‑T1 and ppGalNAc‑T2 on the initiation of O‑glycosylation, siRNA‑ppGalNAc‑T1 (si‑T1) and siRNA‑ppGalNAc‑T2 (si‑T2) were transfected into highly‑invasive estrogen receptor‑negative MDA‑MB‑231 cells to inhibit O‑glycosylation...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393189/upregulation-of-heme-oxygenase-1-in-kupffer-cells-blocks-mast-cell-degranulation-and-inhibits-dendritic-cell-migration-in%C3%A2-vitro
#12
Yuan-Yuan Ma, Mu-Qing Yang, Zhi-Gang He, Mao-Hong Fan, Man Huang, Fei Teng, Qing Wei, Ji-Yu Li
Kupffer cells (KCs) influence liver allografts by interacting with other non‑parenchymal cells. However, the exact mechanism remains unclear. Upregulation of heme oxygenase-1 (HO-1) in KCs upon interaction with mast cells (MCs), and the effects on dendritic cell (DC) function, were investigated in the present study. KCs, MCs and DCs were prepared from 8‑10‑week‑old C57BL/6 mice. KCs were pretreated with PBS, dimethyl sulfoxide, hemin (50 µM; HO‑1 inducer), and zinc protoporphyrin (50 µM; HO‑1 inhibitor) for 8 h...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393228/microarray%C3%A2-based-screening-of-differentially-expressed-genes-in-glucocorticoid%C3%A2-induced-avascular-necrosis
#13
Gangyong Huang, Yibing Wei, Guanglei Zhao, Jun Xia, Siqun Wang, Jianguo Wu, Feiyan Chen, Jie Chen, Jingshen Shi
The underlying mechanisms of glucocorticoid (GC)‑induced avascular necrosis of the femoral head (ANFH) have yet to be fully understood, in particular the mechanisms associated with the change of gene expression pattern. The present study aimed to identify key genes with a differential expression pattern in GC‑induced ANFH. E‑MEXP‑2751 microarray data were downloaded from the ArrayExpress database. Differentially expressed genes (DEGs) were identified in 5 femoral head samples of steroid‑induced ANFH rats compared with 5 placebo‑treated rat samples...
April 5, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393182/expression-of-tgf-%C3%AE-1-mtor-signaling-pathway-in-pathological-scar-fibroblasts
#14
Xiao-Xiang Zhai, Zhi-Ming Tang, Ji-Cun Ding, Xiao-Lan Lu
The aim of the present study was to detect the expression of the key molecules, including transforming growth factor‑β1 (TGF-β1), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) of TGF‑β1/mammalian target of rapamycin (mTOR) pathway in pathological scar fibroblasts. Immunofluorescence, reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis were used to detect the expression of the key molecules TGF‑β1, PI3K, Akt, mTOR in fibroblasts of normal skin tissue and pathological scar tissue...
April 5, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393244/constitutive-androstane-receptor-activation-promotes-bilirubin-clearance-in-a-murine-model-of-alcoholic-liver-disease
#15
Xiuyan Wang, Liyu Zheng, Jinming Wu, Binbin Tang, Mengqin Zhang, Debin Zhu, Xianfan Lin
Increased plasma levels of bilirubin have been reported in rat models and patients with alcoholic liver disease (ALD). The constitutive androstane receptor (CAR) is a known xenobiotic receptor, which induces the detoxification and transport of bilirubin. In the present study, the bilirubin transport regulatory mechanisms, and the role of CAR activation in hepatic and extrahepatic bilirubin clearance were investigated in a murine model of ALD. The mice were fed a Lieber-DeCarli ethanol diet or an isocaloric control diet for 4 weeks, followed by the administration of CAR agonists, 1,4-bis-[2‑(3,5-dichlorpyridyloxy)]benzene (TCPOBOP) and phenobarbital (PB), and their vehicles to examine the effect of the pharmacological activation of CAR on serum levels of bilirubin and on the bilirubin clearance pathway in ALD by serological survey, western blotting and reverse transcription‑quantitative polymerase chain reaction...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393225/effect-of-p53%C3%AE-on-human-gastric-cancer-cells-treated-with-recombinant-mutated-human-tnf-and-cisplatin
#16
Wansheng Ji, Mingliang Yuan, Li Zhang, Hongmei Zhang, Jianxin Jiao, Zhixing Gao
The present study aimed to investigate the role of tumour protein 53 isoform b (p53β) on human gastric cancer (GC) cell lines treated with recombinant mutated human tumour necrosis factor (rmhTNF) and cisplatin. The Cell Counting Kit‑8 assay was used to assess growth in the GC cell lines MKN45 and SGC7901, following treatment with rmhTNF in the presence or absence of cisplatin. Levels of p53β and bcl‑2 apoptosis regulator (bcl‑2) mRNA were assessed using reverse transcription‑polymerase chain reaction...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393214/a-novel-mutation-c-121%C3%A2-13t-a-in-the-polypyrimidine-tract-of-the-splice-acceptor-site-of-intron-2-causes-exon-3-skipping-in-mitochondrial-acetoacetyl-coa-thiolase-gene
#17
Yuka Aoyama, Hideo Sasai, Elsayed Abdelkreem, Hiroki Otsuka, Mina Nakama, Sandeep Kumar, Shrikiran Aroor, Anju Shukla, Toshiyuki Fukao
Mitochondrial acetoacetyl-CoA thiolase (T2) (gene symbol: ACAT1) deficiency is an autosomal recessive disorder affecting isoleucine catabolism and ketone body utilization. In this study, mutational analysis of an Indian T2-deficient patient revealed a homozygous mutation (c.121‑13T>A) located at the polypyrimidine tract of the splice acceptor site of intron 2, and exon 3 skipping was identified by cDNA analysis using cycloheximide. We made three mutant constructs (c.121‑13T>A, T>C, and T>G substitutions) followed by making a wild-type minigene construct that included an ACAT1 segment from exon 2 to 4 for a splicing experiment...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393210/cardiac-resident-macrophages-are-involved-in-hypoxia%C3%A2-induced-postnatal-cardiomyocyte-proliferation
#18
Bo Liu, Hua-Gang Zhang, Yun Zhu, Yun-Han Jiang, Gui-Ping Luo, Fu-Qin Tang, Zhao Jian, Ying-Bin Xiao
Induction of cardiomyocyte proliferation, the most promising approach to reverse myocardial attrition, has been gaining importance as a therapy for cardiovascular disease. Hypoxia and macrophages were previously independently reported to promote cardiomyocyte proliferation in mice. However, whether hypoxia promotes cardiomyocyte proliferation in humans, and the association between hypoxia and macrophages in cardiomyocyte proliferation, have not to the best of our knowledge been previously investigated. The present study investigated the cardiomyocyte proliferation in 22 acyanotic and 29 cyanotic patients...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393195/induction-of-mmp%C3%A2-1-and-%C3%A2-3-by-cyclical-mechanical-stretch-is-mediated-by-il%C3%A2-6-in-cultured-fibroblasts-of-keratoconus
#19
Gen-Lai Du, Wei-Yi Chen, Xiao-Na Li, Rui He, Peng-Fei Feng
In order to understand the effect of mechanical stretch on corneal extracellular matrix remodeling, human keratoconus fibroblasts (HKCFBs) were subjected to cyclic stretch in vitro and the expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and inflammatory cytokines were evaluated. HKCFBs were seeded into a flexible membrane base and subjected to a cyclic stretch regimen of 10% equibiaxial stretch at a stretching frequency of 1 Hz for 6 h using a Flexcell tension unit...
April 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28393187/generation-and-characterization-of-induced-pluripotent-stem-cells-from-guinea-pig-fetal-fibroblasts
#20
Yuehong Wu, Ouyang Li, Chengwen He, Yong Li, Min Li, Xiaoming Liu Liu, Yujiong Wang, Yulong He
Induced pluripotent stem cells (iPS) represent an important tool to develop disease‑modeling assays, drug testing assays and cell‑based replacement therapies. The application of iPS in these fields requires the development of suitable animal models. Of the suitable species, guinea pigs are particularly important and offer significant advantages. Successful iPS generation has been accomplished in a number of species; however, it has not been reported in the guinea pig. The present study successfully generated iPS from guinea pigs (giPS) using single polycistronic virus transduction with mouse octamer‑binding transcription factor 4 (Oct4), sex determining region Y‑box 2 (Sox2), Kruppel‑like factor 4 and c‑Myc...
April 4, 2017: Molecular Medicine Reports
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