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Molecular Medicine Reports

Yang Yang, Zhenghao Zhao, Ni Hou, Yulong Li, Xiaofei Wang, Fei Wu, Ruifang Sun, Jia Han, Hongfei Sun, Tusheng Song, Chen Huang, Yuan Shao
MicroRNAs (miRNAs/miRs) are crucial molecules that act as tumor suppressor genes or oncogenes in human cancer progression. The dysregulation of miRNA expression has been detected in liver cancer. The present study aimed to explore the molecular mechanisms by which miR‑214 affects liver cancer cell proliferation. Reverse transcription‑quantitative polymerase chain reaction was used to determine the expression of miR‑214 in liver cancer cell lines and hepatocellular carcinoma (HCC) tissues. A luciferase reporter assay was performed to determine whether Wnt3a is a target gene of miR‑214...
September 13, 2017: Molecular Medicine Reports
Qinghua Ye, Lei Lei, Lingyun Shao, Jing Shi, Jun Jia, Xiaowen Tong
The effects of microRNA‑141 (miR‑141) on epithelial‑mesenchymal transition (EMT), and ovarian cancer cell migration and invasion were investigated. SKOV3 cells were transfected with the miR‑141 mimic (mimic group), inhibitor (inhibitor group) and nonspecific sequences (NC group), and left untransfected group (blank group). The reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of miR‑141 in SKOV3 cell lines. Then, mRNA levels and protein expression of EMT markers were determined by RT‑qPCR and western blotting, respectively...
September 13, 2017: Molecular Medicine Reports
Yuxin Chen, Ling Zhu, Xi Yang, Cheng Wei, Chuanrong Chen, Yang He, Zhaoning Ji
Ailanthone is a major quassinoid extracted from the Chinese medicinal herb Ailanthus altissima, which has been reported to exert antiproliferative effects on various cancer cells. The present study aimed to investigate the antitumor effects of ailanthone on SGC‑7901 cells, and to analyze its underlying molecular mechanisms. Following treatment with ailanthone, Cell Counting kit‑8 was used to detect the cytotoxic effects of ailanthone on SGC‑7901 cells in vitro. The typical apoptotic morphology of SGC‑7901 cells was observed by Hoechst 33258 staining...
September 13, 2017: Molecular Medicine Reports
Wen-Dong Xiong, Jian Gong, Chao Xing
The primary aim of the current study was to investigate the antitumor effects of ferruginol in OVCAR‑3 human ovary cancer cells. The effects of ferruginol on cell apoptosis, cell migration and cell cycle phase distribution were also evaluated. Cell cytotoxicity induced by ferruginol was determined by an MTT assay, while fluorescence microscopy and transmission electron microscopy (TEM) were performed to investigate apoptotic effects. Flow cytometry was employed to determine the effects of ferruginol on the cell cycle and an in vitro wound healing assay was performed to investigate effects on cancer cell migration...
September 13, 2017: Molecular Medicine Reports
Shiqi She, Qucheng Wei, Bo Kang, Ying-Jie Wang
Embryonic stem cells (ESCs) have unlimited expansion potential and the ability to differentiate into all somatic cell types for regenerative medicine and disease model studies. Octamer‑binding transcription factor 4 (OCT4), encoded by the POU domain, class 5, transcription factor 1 gene, is a transcription factor vital for maintaining ESC pluripotency and somatic reprogramming. Many studies have established that the cell cycle of ESCs is featured with an abbreviated G1 phase and a prolonged S phase. Changes in cell cycle dynamics are intimately associated with the state of ESC pluripotency, and manipulating cell‑cycle regulators could enable a controlled differentiation of ESCs...
September 13, 2017: Molecular Medicine Reports
Qinmiao Huang, Yiming Zeng, Huihuang Lin, Huaping Zhang, Dongyong Yang
Lung cancer is reported to be a major public health issue worldwide and the overall prognosis of patients remains poor. The expression levels of Livin and Survivin, of the inhibitors of apoptosis (IAP) family, are associated with prognostic significance in the majority of solid tumors. Therefore, in the presents study, short hairpin (sh)RNA expression vectors inhibiting the Livin and Survivin genes were constructed to examine the effects of the transfection of Livin shRNA and/or Survivin shRNA on the biological functions of tumor cells...
September 13, 2017: Molecular Medicine Reports
Jian-Hua Wang, Lin-Jing Yuan, Rong-Xin Liang, Zi-Gang Liu, Bo-Hai Li, Zhe-Sheng Wen, Shu-Ting Huang, Min Zheng
The authors' previous study demonstrated that Golgi phosphoprotein 3 (GOLPH3) was significantly overexpressed in esophageal squamous cell carcinoma (ESCC), correlating with poor patient survival. In the present study, GOLPH3 stable overexpression and knockdown KYSE‑140 cell lines were constructed. Cell proliferation, colony formation, cell cycle progression and tumorigenesis assays were performed. The results revealed that GOLPH3 promoted ESCC cell growth and proliferation. The effects of GOLPH3 on the mechanistic target of rapamycin (mTOR) and Wnt/β‑catenin signaling pathways were investigated using western blot analyis and dual‑luciferase reporter assays, and were observed to be activated in cells with GOLPH3 overexpression...
September 13, 2017: Molecular Medicine Reports
Qing Zhang, Shu Ding, Huilin Zhang
Hematopoietic progenitor kinase 1 (HPK1), also known as mitogen‑activated protein kinase kinase kinase kinase 1 is a serine/threonine protein kinase. It is involved in various cellular events, including mitogen‑activated protein kinase signaling, nuclear factor‑κB signaling, cytokine signaling, cellular proliferation and apoptosis, T cell receptor/B cell receptor signaling and T/B/dendritic cell‑mediated immune responses. Therefore, HPK1 has variety of roles in immunity, and is associated with the pathogenesis of autoimmune diseases, cancer, and the inflammatory response...
September 13, 2017: Molecular Medicine Reports
Zhonglin Cai, Haidi Lv, Wenjuan Cao, Chuan Zhou, Qiangzhao Liu, Hui Li, Fenghai Zhou
Prostate cancer (PCa) poses a high risk to older men and it is the second most common type of male malignant tumor in western developed countries. Additionally, there is a lack of effective therapies for PCa at advanced stages. Novel treatment strategies such as adenovirus‑mediated gene therapy and virotherapy involve the expression of a specific therapeutic gene to induce death in cancer cells, however, wild‑type adenoviruses are also able to infect normal human cells, which leads to undesirable toxicity...
September 13, 2017: Molecular Medicine Reports
Jie Liu, Gang Zhan, Dewei Chen, Jian Chen, Zhi-Bin Yuan, Er-Long Zhang, Yi-Xing Gao, Gang Xu, Bing-Da Sun, Wenting Liao, Yu-Qi Gao
Hypoxic preconditioning (HPC) is well‑known to exert a protective effect against hypoxic injury; however, the underlying molecular mechanism remains unclear. The present study utilized a serum metabolomics approach to detect the alterations associated with HPC. In the present study, an animal model of HPC was established by exposing adult BALB/c mice to acute repetitive hypoxia four times. The serum samples were collected by orbital blood sampling. Metabolite profiling was performed using ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOFMS), in conjunction with univariate and multivariate statistical analyses...
September 13, 2017: Molecular Medicine Reports
Xiao-Wei Cai, Rong Zhu, Lei Ran, Yi-Qian Li, Ke Huang, Jing Peng, Wei He, Chun-Li Zhou, Ru-Peng Wang
It is widely accepted that keratinocytes act as non‑professional antigen‑presenting cells and support superantigen‑induced proliferation of resting T cells; however, it remains unknown whether keratinocytes function in situ with T cells via a non‑contact mechanism. The current study used a transwell co‑culture system and demonstrated, for the first time to the best of the authors' knowledge, that HaCaT cells (the human keratinocyte cell line) did induce T cell proliferation via indirect contact. The data further indicated that exosomes, small membrane vesicles that transfer antigens to recipient cells, are also involved in the superantigen‑associated immunity of keratinocytes...
September 13, 2017: Molecular Medicine Reports
Bohan Dong, Guangli Dai, Lei Xu, Damin Shi
Tumor cell lysates (TCLs) have been reported to induce antitumor immunity; however, it remains unclear which elements serve a role in this process. The present study identified 768 proteins that were upregulated in TCL prepared from Lewis lung cancer cells compared with the lysate from type II alveolar epithelial cells. Among the proteins that were upregulated in TCL, receptor for activated C kinase 1 (RACK1) and catenin β‑like 1 (CTNNBL1) are closely associated with cell proliferation and the inhibition of apoptosis...
September 13, 2017: Molecular Medicine Reports
Fei Dong, Guili Zhang, Xia Zhang, Xuena Liu, Na Wang, Chengming Sun
The aim of the present study was to examine the expression and significance of CCAAT/enhancer binding protein α (C/EBPα) and SRY‑related high mobility group box containing transcription factor 4 (SOX4) in chronic myeloid leukemia (CML). Bone marrow samples were collected from patients with CML, and peripheral blood mononuclear cells were collected from healthy controls. Protein and mRNA were extracted from the collected samples, and analyzed using western blotting and reverse transcription‑quantitative polymerase chain reaction analyses, respectively...
September 13, 2017: Molecular Medicine Reports
Jun Yin, Chengyin Weng, Jieke Ma, Fanfan Chen, Yecai Huang, Mei Feng
Previous studies have demonstrated that microRNAs (miRs) are important regulators involved in various cancers, including human glioblastoma (GBM). However, the underlying mechanism of miR‑1288 remains poorly understood, and its role in GBM has not been reported. The present study confirmed that miR‑1288 expression was markedly upregulated in GBM. Ectopic expression of miR‑1288 promoted the prolife-ration, colony formation and anchorage‑independent growth of GBM cells. Bioinformatics analysis coupled with western blotting and luciferase report assays also indicated that miR‑1288 promoted cell proliferation of GBM by targeting ubiquitin carboxyl‑terminal hydrolase (CYLD)...
September 13, 2017: Molecular Medicine Reports
Chui Guo Huang, Feng Xi Li, Song Pan, Chang Bao Xu, Jun Qiang Dai, Xing Hua Zhao
Prostate cancer (CaP) is a serious and common genital tumor. Generally, men with metastatic CaP can easily develop castration‑resistant prostate cancer (CRPC). However, the pathogenesis and tumorigenic pathways of CRPC remain to be elucidated. The present study performed a comprehensive analysis on the gene expression profile of CRPC in order to determine the pathogenesis and tumorigenic of CRPC. The GSE33316 microarray, which consisted of 5 non‑castrated samples and 5 castrated samples, was downloaded from the gene expression omnibus database...
September 13, 2017: Molecular Medicine Reports
Ting-Wen Chung, Yi-Rong Li, Wei Yuan Huang, Jui-Hsin Su, Hong-Lin Chan, Sheng-Hao Lin, Chin-San Liu, Shih-Chao Lin, Chi-Chien Lin, Ching-Hsiung Lin
The dendritic cell (DC) maturation process is essential for the development of T cell responses and immune tolerance. Accordingly, DCs are considered a major target in the development of immunomodulating agents. In the present study, the effect of sinulariolide, an active compound isolated from the cultured soft coral Sinularia flexibilis, on lipopolysaccharide (LPS)‑induced murine bone marrow‑derived DCs was evaluated. The different phenotypes, cytokine secretion and the mix‑lymphocyte reaction of DCs were detected using flow cytometry and ELISA...
September 13, 2017: Molecular Medicine Reports
Mingyue Zhang, Meng Chi, Huichao Zou, Songyu Tian, Zhaodi Zhang, Guonian Wang
Morphine is widely used as an analgesic to treat moderate to severe pain, but chronic morphine use is associated with development of tolerance and dependence, which limits its analgesic efficacy. Our previous research has showed that nonanalgetic dose of a cannabinoid type 2 (CB2) receptor agonist reduced morphine tolerance in cancer pain. A previous study showed the colocalization of CB2 and transient receptor potential vanilloid 1 (TRPV1) in human and rat dorsal root ganglia (DRG) sensory neurons. Whether coadministration of a CB2 receptor agonist and morphine could reduce TRPV1 expression in morphine‑induced antinociception and tolerance in cancer pain is unclear...
September 13, 2017: Molecular Medicine Reports
Jiying Shen, Qiang Yang, Daoyi Yu, Jihong Wu, Yuanfang Zhu, Wenyi Guo
In the current study, it was aimed to evaluate the changes in myelinated and unmyelinated nerve fibers in retinal ischemia‑reperfusion injuries caused by acute ocular hypertension and to determine the sequence of these changes. Adult healthy New Zealand white rabbits were randomized to the hemodynamic group [n=12; used to determine the optimal intraocular pressure (IOP) for the subsequent experiments] and the hypertension group (n=6; 70‑mmHg hypertension induced in one eye). IOP was adjusted using a cannula and saline...
September 12, 2017: Molecular Medicine Reports
Feng Tang, Shengjun Tang, Xiaolong Guo, Chao Yang, Ke Jia
The aim of the present study was to investigate the role of the cancer‑testis antigen family 45 member A1 (CT45A1) in the proliferation, apoptosis, invasion and metastasis of lung cancer cells, and the associated molecular mechanisms. Western blotting determined that the expression of CT45A1 in normal lung cells was far lower than that observed in lung cancer cells. Following the transfection of CT45A1 small (or short) interfering (si)RNA and its negative control into A549 cells using Lipofectamine 2000, the CT45A1 protein and mRNA levels were determined further by western blotting and reverse transcription‑polymerase chain reaction...
September 12, 2017: Molecular Medicine Reports
Tao Liu, Han Zhao, Jianfeng Wang, Xin Shu, Yuan Gao, Xiaoli Mu, Fei Gao, Hongbin Liu
The present study aimed to identify the molecular mechanisms underlying the effects of the fructose‑1,6‑bisphosphatase 1 (FBP1) signaling pathway within normal follicle development and in hyperandrogenism‑induced abnormal follicle growth. To achieve this, murine primary follicles, granulosa cells (GCs) and theca‑interstitial cells (TICs) were isolated, cultured in vitro and treated with a high concentration of androgens. A concentration of 1x10‑5 mol/l testosterone was considerable to induce hyperandrogenism by MTT assay...
September 12, 2017: Molecular Medicine Reports
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