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Molecular Medicine Reports

Chang-Long Zheng, Yong-Mei Fu, Zhan-Xue Xu, Yong Zou, Kai Deng
Hepatitis B virus (HBV) core protein (HBc) serves pivotal roles in the viral life cycle, particularly serving as the basic unit for capsid assembly, and is closely associated with HBV genome replication and progeny virion production. Previous studies have demonstrated that HBc has at least two functional interfaces; two HBc monomers form a homodimer via an intradimer interface, and then 90 or 120 homodimers form an icosahedral capsid via a dimer‑dimer interface. In the present study, the role of the HBc dimer‑dimer interface in HBV replication was investigated...
November 2, 2018: Molecular Medicine Reports
Bingqian Wang, Wenna Li, Hezhi Fang, Huaibin Zhou
Chronic hepatitis B virus (HBV) infection has been reported to be associated with the prevalence of non‑alcoholic fatty liver disease (NAFLD). However, the present study demonstrated that the incidence of fatty liver disease in HBV‑infected subjects (16/152, 10.5%) was not significantly different from in non‑HBV‑infected subjects (292/1,714, 17%), following adjustment for age (odds ratio=0.656; 95% confidence interval=0.379‑1.134; P=0.131). Hepatitis B protein X (HBx) is considered a key regulator in HBV infection and several studies have confirmed that HBx serves a pivotal role in the process of fatty liver disease...
November 2, 2018: Molecular Medicine Reports
Qing Zhang, Xiao-Feng Zhang
Wear particles generated between the interface of joints and artificial joint replacements are one of the primary causes of aseptic loosening. The aim of the present study was to investigate the influence of titanium (Ti) particles on the apoptosis and autophagy of osteoblasts, and probe into the potential use of hyperoside (Hy) as a protector for osteoblasts in Ti particle‑induced injury. MC3T3‑E1 cells were divided into control, Ti, Hy‑1+Ti and Hy‑2+Ti groups. Cell viability was detected using a Cell Counting Kit‑8 assay...
November 2, 2018: Molecular Medicine Reports
Zhouhui Ren, Tong Yang, Pingping Zhang, Kaitai Liu, Weihong Liu, Ping Wang
Spindle and kinetochore‑associated protein 2 (SKA2) is essential for regulating the progression of mitosis. In recent years, SKA2 upregulation has been detected in various human malignancies and the role of SKA2 in tumorigenesis has received increasing attention. However, the expression and functional significance of SKA2 in breast cancer are not completely understood. To study the effects of SKA2 on breast cancer, the expression levels of SKA2 in breast cancer tissues and cell lines were evaluated by western blotting, reverse transcription‑quantitative polymerase chain reaction and immunohistochemical staining...
November 2, 2018: Molecular Medicine Reports
Xiuzhen Hao, Jie Yuan, Huixiao Dong
In previous years, it has been found that Rhodiola has a wide range of pharmacological effects in diseases of the cardiovascular system, as it can remove superoxide anions and hydroxyl radicals in chemical reactions. Behavioral assessment was used to measure cognitive impairment. Inflammation, oxidative stress and caspase‑3 activity were measured using commercial kits. Western blot analysis was used to measure Rho/Rho‑associated kinase (ROCK)/sirtuin 1 (SIRT1)/nuclear factor (NF)‑κB protein expression...
November 2, 2018: Molecular Medicine Reports
Caixia Li, Rong Chen, Caixia Jiang, Li Chen, Zhongping Cheng
Adenomyosis is a common benign disease of women of childbearing age. The typical clinical features are prolonged menstruation, menorrhagia and ingravescent dysmenorrhea. In the present study, the severity of dysmenorrhea was assessed using the visual analogue scale system as follows: 0, No pain; 1‑3, minimal pain; 4‑6, moderate pain; and 7‑10, severe pain. Menstrual blood loss was evaluated using the pictorial blood loss assessment chart (PBAC). Menorrhagia was defined as excessive menstrual blood loss >80 ml (PBAC >100) per period...
November 1, 2018: Molecular Medicine Reports
Longkun Lv, Qiang Wang, Yucheng Yang, Honghai Ji
MicroRNAs (miRNAs) are associated with the initiation and progression of oral squamous cell carcinoma (OSCC) by regulating a variety of cancer‑associated behaviors. Fully understanding the regulatory mechanism of miRNAs in the pathogenesis of OSCC may provide novel promising approaches for the identification of prognostic biomarkers and therapeutic targets for this particular malignancy. In the present study, reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect miRNA (miR)‑495 expression in OSCC tissues and cell lines...
November 1, 2018: Molecular Medicine Reports
Masako Mizuno, Rika Miki, Yoshinori Moriyama, Takafumi Ushida, Kenji Imai, Kaoru Niimi, Tomoko Nakano, Hiroyuki Tsuda, Seiji Sumigama, Eiko Yamamoto, Takeshi Senga, Akira Iwase, Fumitaka Kikkawa, Tomomi Kotani
Recent studies have reported that E2F transcription factor (E2F) 8, an atypical E2F transcription factor, serves a critical role in promoting the growth and development of the murine placenta. However, the function of E2F8 in the human placenta remains unknown. Invasion of extravillous trophoblasts (EVTs) into the maternal decidua is known to be important for the development of the human placenta. To investigate the role of E2F8 in human placental development, E2F8 localisation was examined in the human placenta and E2F8 mRNA expression was detected in primary cultured EVTs...
November 1, 2018: Molecular Medicine Reports
Zhicheng Peng, Xiaobing Li, Dongmei Xing, Xiliang Du, Zhe Wang, Guowen Liu, Xinwei Li
The NOD‑like receptor family pyrin domain containing 3 (NLRP3) inflammasome has been reported to contribute to palmitic acid (PA)‑induced lipotoxicity. Nobiletin (Nob) is a polymethoxylated flavonoid derived from citrus fruits that has been reported to exert antioxidant and antitumor effects. However, its protective and regulatory mechanisms in PA‑induced lipotoxicity remain unclear. Therefore, the aim of the present study was to investigate the protective effects of Nob in AML‑12 cells against lipotoxicity and examine the underlying mechanism...
October 31, 2018: Molecular Medicine Reports
Guangfa Zhu, Wenmei Zhang, Yan Liu, Shenghao Wang
Pulmonary arterial hypertension (PAH) is a clinical hemodynamic syndrome. It is characterized by elevated PA pressure and pulmonary vascular resistance. In the present study, the role of microRNA (miRNA/miR)‑371b‑5p in monocrotaline‑induced PAH and the underlying mechanisms were investigated. In a monocrotaline‑induced PAH rat model, gene chip and reverse transcription‑quantitative polymerase chain reaction were employed to measure miRNA expression levels. The results revealed that miR‑371b‑5p was downregulated in PAH rats compared with the control group...
October 31, 2018: Molecular Medicine Reports
Dong Chen, Xin Yu
Glioma is among the most common primary brain tumors and one of the most aggressive and lethal forms of human cancer. Long noncoding RNAs (lncRNAs) have demonstrated great importance in the development and progression of cancer. The present study aimed to investigate the role of the novel tumor suppressive lncRNA on Chromosome 8p12 (TSLNC8), in cell proliferation, metastasis and apoptosis in human glioma. It was initially reported that the relative transcript levels of TSLNC8 were significantly decreased in human glioma tissues and cultured glioma cells, as evidenced by RT‑qPCR...
October 30, 2018: Molecular Medicine Reports
Yingjian Gao, Bo Wang, Conghuan Shen, Weiwei Xin
The concept that inflammation serves a leading role in osteoclast‑induced bone loss under pathological circumstances is now widely accepted. In the present study, it was observed that lipopolysaccharides (LPSs) demonstrated a synergic effect on receptor activator of nuclear factor κ‑B ligand (RANKL)‑induced osteoclast differentiation in Raw264.7 cells, with increasing levels of multiple pro‑inflammatory cytokines including interleukin (IL)‑6, tumor necrosis factor‑α and IL‑1β. Furthermore, microRNA (miR)‑146a was highly induced by LPS and RANKL co‑stimulation during the process of osteoclast differentiation...
October 30, 2018: Molecular Medicine Reports
Ping He, Xun Sun, Hong-Jing Cheng, Ya-Bin Zou, Quan Wang, Chang-Li Zhou, Wan-Qi Liu, Yue-Ming Hao, Xiang-Wei Meng
Small ubiquitin‑like modifier proteins are involved in tumorigenesis; however, the potential effects and functions of the family member ubiquitin‑like modifier‑activating enzyme 2 (UBA2) on colorectal cancer are not clear. The present study aimed to examine the effects of UBA2 on the proliferation of colorectal cancer cells in vitro and in vivo. The mRNA and protein expression levels of UBA2 in patients with colorectal cancer were measured by reverse transcription‑quantitative polymerase chain reaction and immunohistochemistry, respectively...
October 30, 2018: Molecular Medicine Reports
Huan Zhou, Hui-Jing Zhang, Lin Guan, Yi-Ning Zhang, Yue Li, Ming-Jun Sun
Inflammatory bowel disease (IBD) is a type of chronic inflammatory disturbance that affects a number of individuals worldwide; the precise mechanism is unclear and treatment is frequently insufficient to maintain patients in remission. Saccharomyces boulardii is a thermophilic, non‑pathogenic yeast that may be administered for prophylaxis and treatment of a variety of diarrheal diseases. Recent clinical studies have demonstrated that it may have a role in IBD; however, the mechanism of action is unclear...
October 30, 2018: Molecular Medicine Reports
Hua Liu, Wei Wei, Xiaojian Wang, Xiaojun Guan, Qingqing Chen, Zhongjin Pu, Xudong Xu, Aichun Wei
Osteosarcoma (OS) is the most common primary malignant bone tumor and the third most common cancer that occurs during childhood and adolescence. Increasing evidence has suggested that microRNA (miR)‑23b‑3p has an important role in OS tumorigenesis; however, the underlying molecular mechanisms remain unknown. The aim of the present study was to investigate the expression levels of miR‑23b‑3p and sine oculis homeobox homolog 1 (SIX1) in OS tissues and cell lines (MG‑63, SaOS‑2 and U2OS), as well as to observe the effects of miR‑23b‑3p on U2OS cell viability, cell cycle, apoptosis and invasive ability...
October 30, 2018: Molecular Medicine Reports
Xiao-Jie Ning, Xiong Yan, Yan-Feng Wang, Ren Wang, Xiao-Li Fan, Zi-Biao Zhong, Qi-Fa Ye
Hepatic ischemia/reperfusion injury (HIRI) serves a causative role in postoperative hepatocyte death; however, the mechanisms underlying HIRI remain unclear. Mitochondrial autophagy, with apoptosis, cell cycle distribution and DNA damage repair, may be regarded as a regulatory factor post‑HIRI. Parkin, a novel ubiquitin ligase, has been reported to increase mitochondrial autophagy and decrease apoptosis. However, the association between Parkin, mitochondrial autophagy and other regulatory factors in HIRI is unclear...
October 29, 2018: Molecular Medicine Reports
Meng Li, Huijuan Fang, Jian Hu
Apelin has been reported to be associated with multiple physiological processes in the cardiovascular system. The aim of the present study was to investigate the effects of Apelin‑13 administration on cardiac function, hyperglycemia, insulin resistance (IR), dyslipidemia, endothelial function, inflammation and glucose metabolism in type 2 diabetic Goto‑Kakizaki (GK) rats, and compare the protective effects of Apelin‑13 with metformin or atorvastatin. In the present study, type 2 diabetes was induced in male Goto‑Kakizaki (GK) rats fed with high‑fat diet (HFD)...
October 29, 2018: Molecular Medicine Reports
Yang Yang, De-Ming Sun, Jun-Feng Yu, Man Zhang, Cheng Yi, Rui Yang, Bao-Hua Dan, Ai-Jun Li
Long noncoding RNA taurine upregulated gene 1 (lncRNA TUG1) and microRNA‑196a (miR‑196a) have been reported to serve important roles in the development of renal cell carcinoma (RCC). However, their potential mechanisms have not been completely elucidated. The aim of the present study was to clarify the biological functions of lncRNA‑TUG1 and miR‑196a, in addition to investigating the interaction between lncRNA‑TUG1 and microRNA‑196a, providing a novel insight into RCC tumorigenesis. The present study comprised two parts...
October 29, 2018: Molecular Medicine Reports
Lan Liu, Yanxin Huang, Kaili Zhang, Shupeng Song, Shuangxing Li, Yongguo Li, Yinghua Lan
Hepatitis B core antigen (HBcAg) possesses unusual immunologic features. However, the biological roles and mechanisms of HBcAg in dendritic cell proliferation and apoptosis remain to be elucidated. In the present study, DC2.4 cells were treated with different concentrations of HBcAg (10, 20 and 30 µg/ml). MTT assay and flow cytometry (Annexin V/propidium iodide analysis) were performed to investigate changes in cell proliferation and apoptosis. Western blot analysis was conducted to examine the changes in nuclear factor (NF)‑κB and protein kinase C (PKC) signaling pathways...
October 26, 2018: Molecular Medicine Reports
Chang-Hui Wang, Tong-Cui Jiang, Wei-Min Qiang, Li Zhang, Li-Jie Feng, Yu-Jun Shen, Yu-Xian Shen
Mesencephalic astrocyte‑derived neurotrophic factor (MANF) is an endoplasmic reticulum stress‑inducible protein, which has been suggested to be upregulated in inflammatory diseases; however, how inflammation regulates its transcription remains unclear. Activator protein‑1 (AP‑1), which is a transcription factor complex composed of c‑Fos and c‑Jun, is activated during the inflammatory process. The present study aimed to investigate whether the AP‑1 complex regulates MANF transcription. The results of a luciferase reporter assay revealed that one of three putative AP‑1 binding sites in the MANF promoter region is essential for enhancement of MANF transcription...
October 26, 2018: Molecular Medicine Reports
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