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Molecular Medicine Reports

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https://www.readbyqxmd.com/read/28498449/bioinformatic-analysis-of-the-effects-and-mechanisms-of-decitabine-and-cytarabine-on-acute-myeloid-leukemia
#1
Shiyong Zhou, Pengfei Liu, Huilai Zhang
Acute myeloid leukemia (AML) is a frequently occurring malignant disease of the blood and may result from a variety of genetic disorders. The present study aimed to identify the underlying mechanisms associated with the therapeutic effects of decitabine and cytarabine on AML, using microarray analysis. The microarray datasets GSE40442 and GSE40870 were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) and differentially methylated sites were identified in AML cells treated with decitabine compared with those treated with cytarabine via the Linear Models for Microarray Data package, following data pre‑processing...
May 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498411/mitochondrial-activity-and-oxidative-stress-functions-are-influenced-by-the-activation-of-ahr-induced-cyp1a1-overexpression-in-cardiomyocytes
#2
Bing Zhou, Xi Wang, Feng Li, Yingting Wang, Lei Yang, Xiaolong Zhen, Wuhong Tan
There is an endemic cardiomyopathy currently occurring in China, termed, Keshan disease (KD). The authors previously compared mitochondrial‑associated gene expression profiles of peripheral blood mononuclear cells (PBMCs) derived from KD patients and normal controls, using mitochondria‑focused cDNA microarray technology. The results detected an upregulation of the enzyme‑associated CYP1A1 gene, (ratios ≥2.0). The aryl hydrocarbon receptor (AhR) regulates the expression of numerous cytochrome P450 (CYP) genes including members of the CYP1 family; CYP1A1 and CYP1A2...
May 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498401/luteolin-induced-apoptosis-through-activation-of-endoplasmic-reticulum-stress-sensors-in-pheochromocytoma-cells
#3
Kisang Kwon, Young-Sook Kwon, Seung-Whan Kim, Kweon Yu, Kyung-Ho Lee, O-Yu Kwon
Luteolin [2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-chromenone] is an active flavonoid compound from Lonicera japonica (Caprifoliaceae). Luteolin inhibits tumor cell proliferation, inflammatory and oxidative stress better, when compared with other flavonoids. In the present study, it was demonstrated that luteolin induces typical apoptosis in PC12 cells (derived from a pheochromocytoma of the rat adrenal medulla) accompanied by DNA fragmentation and formation of apoptotic bodies. In addition, luteolin regulates expression of the endoplasmic reticulum (ER) chaperone binding immunoglobulin protein, activating ER stress sensors (eukaryotic initiation factor 2α phosphorylation and X‑box binding protein 1 mRNA splicing) and induced autophagy...
May 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498471/sodium-tanshinone-iia-sulfonate-improves-inflammation-aortic-endothelial-cell-apoptosis-disseminated-intravascular-coagulation-and-multiple-organ-damage-in-a-rat-heat-stroke-model
#4
Fang Chen, Huimin Li, Guoguo Zhu, Xiaojuan Chen, Zhongzhi Tang
The aim of the present study was to investigate the effects of sodium tanshinone IIA sulfonate (STS) on inflammatory responses, aortic endothelial cell apoptosis, disseminated intravascular coagulation (DIC) and multiple organ damage in an animal model of classic heat stroke (CHS). The rats in the heat stroke (HS) and STS‑treated heat stroke (STS‑HS) groups were placed into a pre‑warmed animal temperature controller (ATC) at 35˚C. The moment at which the rectal temperature reached 43.5˚C was considered as the time of onset of HS...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498461/increased-production-of-human-fibroblast-growth-factor-17-in-escherichia-coli-and-proliferative-activity-in-nih3t3-cells
#5
Meiyu Wu, Na Song, Jiliang Cheng, Yang Zhao, Nazi Chen, Jisheng Ma, Xiaokun Li, Chao Jiang, Haijun Wang
Fibroblast growth factor 17 (FGF17) is a novel member of the FGFs family, which is essential for cell development, tissue repair, tumor growth and invasion. The aim of the current study was to obtain a high expression level of recombinant human FGF17 (rhFGF17), including soluble proteins and inclusion bodies. An optimized rhFGF17 cDNA sequence was cloned into a pET3a vector, then the pET3a‑hFGF17 vector was transformed into BL21(DE3)pLysS Escherichia coli cells. Expression was induced by optimizing the conditions using isopropyl β‑D‑1‑thiogalactopyranoside (IPTG) and it was confirmed that a 24‑h exposure to 0...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498448/sanguinarine-protects-against-ovariectomy%C3%A2-induced-osteoporosis-in-mice
#6
Yan Ma, Junjie Chu, Jianjun Ma, Lei Ning, Ke Zhou, Xiangqian Fang
Natural compounds are alternative agents that have therapeutic potential for preventing and treating osteoporosis. Traditionally, sanguinarine has been used clinically due to its diverse biological properties, including antimicrobial, anti‑inflammatory and anticancer effects. Recently, for the first time, it was reported that sanguinarine inhibits osteoclast differentiation and bone resorption by suppressing the tumor necrosis factor ligand superfamily member 11‑induced nuclear factor‑κB and extracellular signal‑regulated kinase signaling pathways in vitro...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498447/dec2-expression-antagonizes-cisplatin%C3%A2-induced-apoptosis-in-human-esophageal-squamous-cell-carcinoma
#7
Hidenobu Sato, Yunyan Wu, Yukio Kato, Qiang Liu, Hideaki Hirai, Tadashi Yoshizawa, Satoko Morohashi, Jun Watanabe, Hiroshi Kijima
Differentiated embryonic chondrocyte expressed gene 1 (DEC1) and differentiated embryonic chondrocyte expressed gene 2 (DEC2) belong to the Hairy/Enhancer of Split subfamily of basic helix‑loop‑helix factors. Previous studies have demonstrated that DEC proteins are involved in the regulation of circadian rhythms, response to hypoxia, and tumorigenesis. However, the roles of DEC1 and DEC2 in apoptosis of esophageal carcinoma remain unclear. In the present study, alterations in expression of apoptosis‑related markers in human esophageal squamous cell carcinoma TE‑11 cells treated with cisplatin were examined by western blot, while overall cell viability and apoptosis were analyzed by MTS assay and hematoxylin and eosin staining, respectively...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498446/synergistic-protective-effect-of-fty720-and-vitamin-e-against-simulated-cerebral-ischemia-in-vitro
#8
Xin Pang, Xuening Hou
The purpose of the present study was to explore the combination effect of FTY720 and vitamin E on cerebral ischemia. Astrocytes were isolated from newborn Sprague‑Dawley rats and were subjected to FTY720, vitamin E, or combination of the two. The astrocyte cultures were then exposed to oxygen‑glucose deprivation (OGD) to simulate an ischemic model in vitro. Cell viability, lactate dehydrogenase (LDH) leakage and cell apoptosis were detected following 12 h of exposure to OGD. In addition, the levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑1β, total antioxidant capacity, intercellular adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, chemokine (C‑X‑C motif) ligand (CXCL)‑10, heme oxygenase (HO)‑1 and superoxide dismutase (SOD)‑1 were measured...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498426/identification-of-a-missense-hoxd13-mutation-in-a-chinese-family-with-syndactyly-type-i-c-using-exome-sequencing
#9
Hao Deng, Ting Tan, Quanyong He, Qiongfen Lin, Zhijian Yang, Anding Zhu, Liping Guan, Jingjing Xiao, Zhi Song, Yi Guo
Syndactyly is one of the most common hereditary limb malformations, and is characterized by the fusion of specific fingers and/or toes. Syndactyly type I‑c is associated with bilateral cutaneous or bony webbing of the third and fourth fingers and occasionally of the third to fifth fingers, with normal feet. The aim of the present study was to identify the genetic basis of syndactyly type I‑c in four generations of a Chinese Han family by exome sequencing. Exome sequencing was conducted in the proband of the family, followed by direct sequencing of other family members of the same ancestry, as well as 100 ethnically‑matched, unrelated normal controls...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498421/molecular-analysis-of-the-mouse-brain-exposed-to-chronic-mild-stress-the-influence-of-hepatocyte-nuclear-factor-4%C3%AE-on-physiological-homeostasis
#10
Kaoru Ikubo, Kyosuke Yamanishi, Nobutaka Doe, Takuya Hashimoto, Miho Sumida, Yuko Watanabe, Yosif El-Darawish, Wen Li, Haruki Okamura, Hiromichi Yamanishi, Hisato Matsunaga
Major depressive disorder (MDD) is a prevalent disorder that causes considerable disability in social functioning and is a risk factor for physical diseases. Recent clinical reports have demonstrated a marked association between MDD and physiological dyshomeostasis induced by metabolic disorders, including diabetes, hormone abnormalities and autoimmune diseases. The authors of the present study have previously analyzed comparative gene expression profiles in the prefrontal cortex (PFC) of a chronic mild stress (CMS) animal model of MDD...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498398/per2-is-downregulated-by-the-lps-induced-inflammatory-response-in-synoviocytes-in-rheumatoid-arthritis-and-is-implicated-in-disease-susceptibility
#11
Hwayoung Lee, Seong-Su Nah, Sung-Hae Chang, Hyung-Ki Kim, Jun-Tack Kwon, Sanghyun Lee, Ik-Hyun Cho, Sang Won Lee, Young Ock Kim, Seung-Jae Hong, Hak-Jae Kim
The clinical symptoms of rheumatoid arthritis (RA) present with circadian variation, with joint stiffness and pain more prominent in the early morning. The mammalian clock genes, which include circadian locomotor output cycles kaput, brain and muscle Arnt-like protein 1, period and cryptochrome, regulate circadian rhythms. In order to identify the association between genetic polymorphisms in the circadian clock gene period 2 (PER2) and RA, the present study genotyped three PER2 single nucleotide polymorphisms (SNPs), rs934945, rs6754875, and rs2304674, using genetic information from 256 RA patients and 499 control subjects...
May 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498479/ppar-%C3%AE-improves-the-recovery-of-lung-function-following-acute-respiratory-distress-syndrome-by-suppressing-the-level-of-tgf-%C3%AE-1
#12
Yang Liu, Liping Xie, Mingquan Yang, Xiaofei Tan, Yonghong Zeng, Gang Zheng, Youying Chen, Ping Chen
Although peroxisome proliferator-activated receptor (PPAR)-α has been reported to be involved in preventing acute lung injury (ALI), the molecular regulation of post‑ALI lung recovery remains to be fully elucidated. The aim of the present study was to characterize the mechanism by which PPAR‑α prevents ALI and examine the role of PPAR‑α in the recovery of lung function following acute respiratory distress syndrome (ARDS). Reverse transcription‑quantitative‑polymerase chain reaction and western blot analyses suggested that PPAR‑α was effective in suppressing transforming growth factor (TGF)‑β1 in HLF cells and RAW 264...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498476/effects-of-unfractionated-heparin-and-rivaroxaban-on-the-expression-of-heparanase-and-fibroblast-growth-factor-2-in-human-osteoblasts
#13
Jingjun Xia, Wei Sheng, Liubao Pei, Ning Li, Zhipeng Zhang, Jialiang Wang, Jianing Zu, Nanxiang Wang, Duanyang Wang
Long-term treatment with anticoagulants may contribute to osteoporosis. Although unfractionated heparin and rivaroxaban have adverse effects on bone microstructure and function in adult rats, the underlying mechanism remains to be elucidated. Heparanase (HPSE) and fibroblast growth factor (FGF)2 are important signals in bone formation and fracture healing. Therefore, the present study was designed to investigate the effects of unfractionated heparin and rivaroxaban on the expression of HPSE and FGF2 in human osteoblasts...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498469/expression-of-c-flip-in-a-rat-model-of-sepsis-and-its-effects-on-endothelial-apoptosis
#14
Lei Shen, Zhengda Sun, Feng Zhao, Wei Wang, Wenhong Zhang, Hechen Zhu
Sepsis is characterized by the impaired regulation of inflammatory responses. Apoptosis is important in the pathogenesis of sepsis. Cellular FLICE‑inhibitory protein (c‑FLIP) is a catalytically inactive caspase‑8 homologue, which negatively interferes with apoptotic signaling. The role of c‑FLIP in sepsis and in endothelial cell apoptosis, a critical step in the pathogenesis of sepsis, remains controversial. In the present study, to investigate the relationship between c‑FLIP and sepsis, a rat model of sepsis was induced by cecal ligation and puncture, and western blot analysis was used to detect the expression of c‑FLIPL, the long isoform of c‑FLIP...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498466/elevated-slc26a4-gene-promoter-methylation-is-associated-with-the-risk-of-presbycusis-in-men
#15
Jin Xu, Jiachen Zheng, Wanjing Shen, Lili Ma, Ming Zhao, Xubo Wang, Jiyuan Tang, Jihong Yan, Zhenhua Wu, Zuquan Zou, Shizhong Bu, Yang Xi
Presbycusis affects approximately one-third of people over the age of 65 and is a worldwide health problem. In the current study, whether the methylation level of solute carrier family 26 member 4 (SLC26A4) predicted an increased risk of presbycusis was investigated. Peripheral blood samples from 102 patients with presbycusis and 104 controls were collected, and the methylation of the CpG sites of SLC26A4 was measured by applying pyrosequencing technology combined with sodium bisulfate DNA conversion chemistry...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498459/inhibitors-of-parp-1-exert-inhibitory-effects-on-the-biological-characteristics-of-hepatocellular-carcinoma-cells-in%C3%A2-vitro
#16
Xiaorong Mao, Senrong Du, Zhongxia Yang, Liting Zhang, Xuebin Peng, Ni Jiang, Haiyu Zhou
It has been confirmed that the inhibitors of poly ADP-ribose polymerF(^9ase‑1 (PARP‑1) can inhibit the proliferation, apoptosis and invasion of tumor cells. However, the effects of inhibitors of PARP‑1 on hepatocellular carcinoma remain to be elucidated. The aim of the present study was to investigate the effect of three types of PARP‑1 inhibitor on the proliferation, apoptosis and migration of hepatocellular carcinoma in vitro. An MTT assay was performed to detect the proliferation of HepG2 cells following treatment with the PARP‑1 inhibitors, AG014699, BSI‑201 and AZD‑2281...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498451/differential-effects-of-p38-and-erk1-2-on-the-chondrogenic-and-osteogenic-differentiation-of-dental-pulp-stem-cells
#17
Pengfei Ba, Xiaoyu Duan, Guo Fu, Shuyan Lv, Pishan Yang, Qinfeng Sun
The extracellular signal-regulated protein kinase 1/2 (Erk1/2) and p38 mitogen‑activated protein‑kinase pathways serve important roles in the regulation of osteogenic and chondrogenic differentiation in mesenchymal stem cells (MSCs). However, the exact mechanism remains unclear, and the effect is controversial. In the present study, the effects of Erk1/2 and p38 on the osteogenic and chondrogenic differentiation of dental pulp stem cells (DPSCs) were compared in vitro. The results indicated that inhibition of Erk1/2 is able to enhance the osteogenic differentiation of DPSCs and inhibit chondrogenic differentiation, whereas inhibition of p38 demonstrated the opposite effect...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498444/high-expression-of-pftk1-in-cancer-cells-predicts-poor-prognosis-in-colorectal-cancer
#18
Youjun Mao, Yuqing Jia, Huijun Zhu, Wei Wang, Qin Jin, Fang Huang, Shu Zhang, Xiaoqiang Li
The serine/threonine-protein kinase PFTAIRE 1 (PFTK1) is a member of the cyclin‑dependent kinase family that is highly expressed in several malignant tumors, including hepatocellular carcinoma, esophageal, breast and gastric cancers, and glioma. It contributes to tumor progression and influences tumor prognosis. However, the expression and clinicopathological significance of PFTK1 in human colorectal cancer (CRC) remain to be elucidated. The present study aimed to examine the expression of PFTK1 and to evaluate the clinical significance of its expression in human CRC...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498438/dexamethasone-reduces-side-population-fraction-through-downregulation-of-abcg2-transporter-in-mcf-7-breast-cancer-cells
#19
Jong Bin Kim, Sung Eun Hwang, Sang-Pil Yoon
Side population (SP) cells represent a rare population among breast cancer cells. SP cells have been reported to act as cancer stem‑like cells, and to participate in the development of multidrug resistance via modulating the expression of ATP-binding cassette subfamily G member 2 (ABCG2). Dexamethasone is a corticosteroid drug that has been used as an adjuvant treatment to enhance the efficacy of chemotherapeutic agents; however, its effects in breast cancer have yet to be thoroughly investigated. In the present study, the effects of dexamethasone were investigated using the human MCF‑7 breast cancer cell line, and SPs were examined in detail...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28498419/mir-590-regulates-wt1-during-proliferation-of-g401-cells
#20
Liyi Hong, Xu Zhao, Xuejun Shao, Hong Zhu
Nephroblastoma (Wilms' tumor) is frequently associated with mortality in children. MicroRNAs (miRNAs) are important for tumor development serving as oncogenes or tumor suppressors. In the present study, miRNA‑590 (miR‑590) was identified to be upregulated in Wilms' tumor tissues compared with the normal adjacent tissues. Additionally, the levels of miR‑590 were consistent with their clinical stage. Wilms' tumor 1 (WT1) was considered to be a tumor suppressor in certain tumor types, and it has been detected at low expression levels in various types of cancer with high cell proliferation and aggressive behavior...
May 10, 2017: Molecular Medicine Reports
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