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Journal of Proteomics

Francesco Finamore, Jean-Luc Reny, Sarah Malacarne, Pierre Fontana, Jean-Charles Sanchez
Diabetes is a major risk factor for cardiovascular diseases. Although aspirin is considered a cornerstone of the prevention and treatment of atherothrombotic-related ischemic events, this antiplatelet drug appears to be less effective in patients with poorly controlled diabetes. It has been suggested that the glycation of platelet proteins plays a pivotal role in poor responsiveness to aspirin. However, a direct effect on the critical residue (serine 529, or Ser 529) of the catalytic pocket of cyclooxygenase 1 (COX-1) has never been demonstrated...
September 18, 2018: Journal of Proteomics
Wen Zhang, Tianqi Zhang, Yun Chen
Cytochrome c (Cyt c) plays an important role in cell apoptosis. However, it could be functionally compromised by interaction with anti-apoptosis proteins, known as protein-protein interactions (PPIs). Among the proteins potentially interacting with Cyt c, both HSP27 and Bcl-xL serve as pivotal anti-apoptosis proteins. Because multiple PPIs, especially those involve the same protein, could affect each other, their simultaneous and quantitative detection is highly needed. In this study, a combined approach of molecularly imprinted polymers (MIPs) and LC-MS/MS-based targeted proteomics was developed for simultaneous quantification of Cyt c-HSP27 and Cyt c-Bcl-xL interactions...
September 17, 2018: Journal of Proteomics
Alberto Valdés, Bodil S Holst, Sebastian Lindersson, Margareta Ramström
Increased knowledge on serum protein profiles during early pregnancy in dogs would be valuable for several reasons, including animal welfare. Inflammatory changes during this period have been described. Today, mass spectrometry (MS) is a well-established technique to perform unbiased qualitative and quantitative studies of proteins in body fluids regardless of species. In the present study, a shotgun proteomic analysis based on nano-liquid chromatography-MS was performed to identify proteins of altered abundance during canine pregnancy, and, thereafter, a targeted parallel reaction monitoring (PRM)-method was developed and applied to absolutely quantify the concentrations of a selection of these proteins...
September 10, 2018: Journal of Proteomics
Pey Yee Lee, Siok-Fong Chin, Teck Yew Low, Rahman Jamal
No abstract text is available yet for this article.
September 10, 2018: Journal of Proteomics
Edgar Neri-Castro, Bruno Lomonte, Mariel Valdés, Roberto Ponce-López, Melisa Bénard-Valle, Miguel Borja, Jason L Strickland, Jason M Jones, Christoph Grünwald, Fernando Zamudio, Alejandro Alagón
Venoms of the three species of Ophryacus (O. sphenophrys, O. smaragdinus, and O. undulatus), a viperid genus endemic to Mexico, were analyzed for the first time in the present work. The three venoms lacked procoagulant activity on human plasma, but induced hemorrhage and were highly lethal to mice. These venoms also displayed proteolytic and phospholipase A2 activities in vitro. The venom of O. sphenophrys was the most lethal and caused hind-limb paralysis in mice. Proteomic profiling of O. sphenophrys venom showed a predominance of metalloproteinase (34...
September 8, 2018: Journal of Proteomics
Surintorn Boonanuntanasarn, Chatsirin Nakharuthai, Denise Schrama, Rungsun Duangkaew, Pedro M Rodrigues
Dietary oils of varying fatty acid composition have been used in tilapia feeds; nevertheless, investigation of their effects on metabolism and physiological processes has been limited. Therefore, in this study, using proteomic technology, the effects of different dietary lipids (DLs) on hepatic lipid metabolism and physiological processes were investigaed in Nile tilapia (Oreochromis niloticus). Fish were fed with different DL, which included palm oil (PO), linseed oil (LO) and soybean oil (SBO) for 90 days...
September 8, 2018: Journal of Proteomics
Daiane Gonzaga Ribeiro, Raphael Ferreira de Almeida, Wagner Fontes, Mariana de Souza Castro, Marcelo Valle de Sousa, Carlos André Ornelas Ricart, Raimundo Nonato Vieira da Cunha, Ricardo Lopes, Jonny Everson Scherwinski-Pereira, Angela Mehta
Oil palm is an oleaginous plant of relevant economic importance since its fruits are rich in vegetable oil. These plants have a single apical meristem and the main method for vegetative propagation is somatic embryogenesis. The aim of this study was to identify differentially abundant proteins from oil palm genotypes contrasting in the capacity of embryogenic competence acquisition, using shotgun proteomics. Oil palm leaves were subjected to callus induction and the material was collected in biological triplicates at 14 and 90 days of callus induction...
September 5, 2018: Journal of Proteomics
Bing Wang, Lin Yang, Yaqi Zhang, Shenglan Chen, Xiang Gao, Cuihong Wan
To adapt to xeric environments, microorganisms have evolved with the capability of the superior desiccation tolerance and rapid resuscitation after rehydration. Nostoc flagelliforme, a representative terrestrial cyanobacterium that is distributed in west and west-northern parts of China, serves as an ideal model for gaining insight in the physiological recovery mechanism. In this study, LC-MS/MS combined with isobaric chemical labeling technique (iTRAQ) was used to quantify dynamic changes of proteins in N...
September 4, 2018: Journal of Proteomics
Mónica R Romero, Andrés Pérez-Figueroa, Mónica Carrera, Willie J Swanson, David O F Skibinski, Angel P Diz
Speciation mechanisms in marine organisms have attracted great interest because of the apparent lack of substantial barriers to genetic exchange in marine ecosystems. Marine mussels of the Mytilus edulis species complex provide a good model to study mechanisms underlying species formation. They hybridise extensively at many localities and both pre- and postzygotic isolating mechanisms may be operating. Mussels have external fertilisation and sperm cells should show specific adaptations for survival and successful fertilisation...
September 3, 2018: Journal of Proteomics
Fernanda Munhoz Dos Anjos Leal Zimmer, Gabriela Prado Paludo, Hercules Moura, John R Barr, Henrique Bunselmeyer Ferreira
Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar. However, M. hyopneumoniae causes porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. M. hyopneumoniae and M. flocculare do not penetrate their host cells, and secreted proteins are important for bacterium-host interplay. Thus, the secretomes of a swine trachea cell line (NPTr) infected with M. hyopneumoniae 7448 (a pathogenic strain), M. hyopneumoniae J (a non-pathogenic strain) and M. flocculare were compared to shed light in bacterium-host interactions...
August 31, 2018: Journal of Proteomics
Juliana R Cunha, Fabrício E L Carvalho, Milton C Lima-Neto, Douglas Jardim-Messeder, João Victor A Cerqueira, Marcio O Martins, Adilton V Fontenele, Márcia Márgis-Pinheiro, Setsuko Komatsu, Joaquim A G Silveira
Chloroplast APX isoforms display controversial roles as H2 O2 scavengers and signaling players in response to abiotic stress and conclusive results are lacking. We tested the hypothesis that thylakoidal APX displays an important role for drought tolerance, especially by regulating abundance of essential protein species. For this, OsApx8 RNAi-silenced rice (apx8) and non-transformed plants (NT) were exposed to mild water deficit. The drought-sensitivity in apx8 plants was revealed by decreases in shoot growth, relative water content and photosynthesis, which was accompanied by increased membrane damage, all compared to NT plants...
August 29, 2018: Journal of Proteomics
Jesus V Jorrin-Novo, Setsuko Komatsu, Rosa Sanchez-Lucas, Luis E Rodríguez de Francisco
In this century we have assisted at an unimaginable expansion of proteomics, with continuous innovations and optimizations in methods, techniques, protocols, equipment, and associated bioinformatics tools. We have moved forward very fast from first (gel electrophoresis based), to second (based on isotopic or isobaric labelling), to third (shotgun or gel-free, label-free), and to fourth (targeted, mass-western, or SRM/MRM) generation techniques. This evolution is clearly observed in the literature since 1994, when the term "proteome" was first coined, with plant proteomics progressing at a much lower speed than human and other model organisms...
August 29, 2018: Journal of Proteomics
Tania Barth, Samuel Coelho Mandacaru, Sébastien Charneau, Marcelo Valle de Souza, Carlos André Ornelas Ricart, Eliane Ferreira Noronha, Amanda Araújo Souza, Sonia Maria de Freitas, Peter Roepstorff, Wagner Fontes, Mariana S Castro, Osmindo Rodrigues Pires Júnior
Spider venoms are composed of a complex mixture of bioactive molecules. The structural and functional characterization of these molecules in the venom of the Brazilian spider Acanthoscurria natalensis, has been little explored. The venom was fractionated using reversed-phase liquid chromatography. The fraction with hyaluronidase activity was named AnHyal. The partial sequencing of AnHyal revealed the presence of a CRISP-like protein, in addition to hyaluronidase, comprising 67% coverage for hyaluronidase from Brachypelma vagans and 82% for CRISP-like protein from Grammostola rosea...
August 27, 2018: Journal of Proteomics
Eelke P Béguin, Bart L van den Eshof, Arie J Hoogendijk, Benjamin Nota, Koen Mertens, Alexander B Meijer, Maartje van den Biggelaar
The vascular endothelium provides a unique interaction plane for plasma proteins and leukocytes in inflammation. The pro-inflammatory cytokines Tumor Necrosis Factor α (TNFα) and interleukin 1β (IL-1β) have a profound effect on endothelial cells, which includes increased levels of adhesion molecules and a disrupted barrier function. To assess the endothelial response to these cytokines at the protein level, we evaluated changes in the whole proteome, cell surface proteome and phosphoproteome after 24 h of cytokine treatment...
August 25, 2018: Journal of Proteomics
Dwight R Causey, Jin-Hyoung Kim, David A Stead, Samuel A M Martin, Robert H Devlin, Daniel J Macqueen
In fish used for food production and scientific research, fast growth can be achieved via selective breeding or induced instantaneously via growth hormone (GH) transgenesis (GHT). The proteomic basis for these distinct routes towards a similar higher phenotype remains uncharacterized, as are associated implications for health parameters. We addressed this knowledge gap using skeletal muscle proteomics in coho salmon (Oncorhynchus kisutch), hypothesising that i) selective breeding and GHT are underpinned by both parallel and unique changes in growth systems, and ii) rapidly-growing fish strains have lowered scope to allocate resources towards immune function...
August 25, 2018: Journal of Proteomics
Abderrahim Benmoussa, Clarisse Gotti, Sylvie Bourassa, Caroline Gilbert, Patrick Provost
Extracellular vesicles (EVs), like exosomes, are small membrane vesicles involved in cell-to-cell communications that modulate numerous biological processes. We previously discovered a new EV subset in milk (sedimenting at 35,000 g; 35 K) that protected its cargo (RNAs and proteins) during simulated digestion and was more enriched in microRNAs than exosomes (sedimenting at 100 K). Here, we used LC-MS/MS to push further the comparison between these two pellets. Commonly used EV markers were not differentially enriched between the pellets, questioning their use with cow's milk EVs...
August 25, 2018: Journal of Proteomics
Gemma Prieto-Bonete, María D Pérez-Cárceles, Antonio Maurandi-López, Cristina Pérez-Martínez, Aurelio Luna
Proteomic techniques in bones forensic samples are increasingly, being applied. The main aim of forensic sciences is the estimation of postmortem interval. Most current techniques are useful for the first post-mortem stages. However, in the case of osseous remains, these techniques may be difficult to use due to the high level of decomposition of the sample. Our objective was to attempt to know whether there is a protein profile in human bone remains that would enable a late postmortem. interval ranging from 5 to 20 years postmortem to be estimated...
August 23, 2018: Journal of Proteomics
Anita Horvatić, Nicolas Guillemin, Haider Kaab, Dorothy McKeegan, Emily O'Reilly, Maureen Bain, Josipa Kuleš, Peter David Eckersall
The inflammatory response in chickens (Gallus Gallus domesticus) is an integral part of the bird's response to infection. Detailing proteomic changes occurring during infection would be beneficial to the poultry industry, offering opportunities for comparative pathophysiological analysis. The objective of this study was to quantify the changes in the plasma proteome in chickens challenged with lipopolysaccharide (LPS), a bacterial endotoxin known to stimulate the host innate immune system. Plasma from chicken (N = 6) challenged with Escherichia coli (LPS) (2 mg/kg body weight) was collected pre (0 h) and at 12, 24, 48, and 72 h post-injection along with plasma from a control group (N = 6) challenged with sterile saline...
August 13, 2018: Journal of Proteomics
(no author information available yet)
No abstract text is available yet for this article.
August 10, 2018: Journal of Proteomics
Bao-Zhen Tang, E Meng, Hua-Jian Zhang, Xiao-Mei Zhang, Sassan Asgari, Ya-Ping Lin, Yun-Ying Lin, Zheng-Qiang Peng, Ting Qiao, Xia-Fang Zhang, You-Ming Hou
The venom apparatus is a conserved organ in parasitoids that shows adaptations correlated with life-style diversification. Combining transcriptomics and label-free quantitative proteomics, here we explored the venom apparatus components of the endoparasitoid Tetrastichus brontispae (Eulophidae), and provide a comparison of the venom apparatus proteomes between its two closely related strains, T. brontispae-Octodonta nipae (Tb-On) and T. brontispae-Brontispa longissima (Tb-Bl). Tb-Bl targets the B. longissima pupa as its habitual host...
August 8, 2018: Journal of Proteomics
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