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Gut Pathogens

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https://www.readbyqxmd.com/read/29643938/a-hybrid-reference-guided-de-novo-assembly-approach-for-generating-cyclospora-mitochondrion-genomes
#1
G R Gopinath, H N Cinar, H R Murphy, M Durigan, M Almeria, B D Tall, A J DaSilva
Cyclospora cayetanensis is a coccidian parasite associated with large and complex foodborne outbreaks worldwide. Linking samples from cyclosporiasis patients during foodborne outbreaks with suspected contaminated food sources, using conventional epidemiological methods, has been a persistent challenge. To address this issue, development of new methods based on potential genomically-derived markers for strain-level identification has been a priority for the food safety research community. The absence of reference genomes to identify nucleotide and structural variants with a high degree of confidence has limited the application of using sequencing data for source tracking during outbreak investigations...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29636824/the-prevalence-of-helicobacter-pylori-infection-and-other-risk-factors-among-mongolian-dyspeptic-patients-who-have-a-high-incidence-and-mortality-rate-of-gastric-cancer
#2
Oyuntsetseg Khasag, Gantuya Boldbaatar, Tserentogtoh Tegshee, Davaadorj Duger, Azzaya Dashdorj, Tomohisa Uchida, Takeshi Matsuhisa, Yoshio Yamaoka
Background: Mongolia has not only the second highest incidence rate but also the highest mortality rate for gastric cancer globally. In addition to gastric cancer, ulcerative disease complications are also life threatening; thus, investigating Helicobacter pylori infection and other risk factors is essential. Results: H.pylori infection was high in tested dyspeptic patients from all parts of Mongolia, with an overall infection rate of 80.0%. Logistic regression analysis showed that H...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29610580/infectious-bursal-disease-virus-inoculation-infection-modifies-campylobacter-jejuni-host-interaction-in-broilers
#3
Li Li, Colin Pielsticker, Zifeng Han, Tereza Kubasová, Ivan Rychlik, Bernd Kaspers, Silke Rautenschlein
Background: Campylobacter jejuni is considered as a chicken commensal. The gut microbiota and the immune status of the host may affect its colonization. Infectious bursal disease virus (IBDV) is an immunosuppressive virus of chickens, which allows secondary pathogens to invade or exacerbates their pathogenesis. To investigate the effect of IBDV-induced immunosuppression on the pathogenesis of C. jejuni , broiler chickens were inoculated with a very virulent (vv) strain of IBDV at 14 days post hatch followed by C...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29581730/the-impact-of-rhodiola-rosea-on-the-gut-microbial-community-of-drosophila-melanogaster
#4
Khachik E Labachyan, Dara Kiani, Evgueni A Sevrioukov, Samuel E Schriner, Mahtab Jafari
Background: The root extract of Rhodiola rosea has historically been used in Europe and Asia as an adaptogen, and similar to ginseng and Shisandra , shown to display numerous health benefits in humans, such as decreasing fatigue and anxiety while improving mood, memory, and stamina. A similar extract in the Rhodiola family, Rhodiola crenulata , has previously been shown to confer positive effects on the gut homeostasis of the fruit fly, Drosophila melanogaster. Although, R. rosea has been shown to extend lifespan of many organisms such as fruit flies, worms and yeast, its anti-aging mechanism remains uncertain...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29556252/genomic-characterization-of-malonate-positive-cronobacter-sakazakii-serotype-o-2-sequence-type-64-strains-isolated-from-clinical-food-and-environment-samples
#5
Gopal R Gopinath, Hannah R Chase, Jayanthi Gangiredla, Athmanya Eshwar, Hyein Jang, Isha Patel, Flavia Negrete, Samantha Finkelstein, Eunbi Park, TaeJung Chung, YeonJoo Yoo, JungHa Woo, YouYoung Lee, Jihyeon Park, Hyerim Choi, Seungeun Jeong, Soyoung Jun, Mijeong Kim, Chaeyoon Lee, HyeJin Jeong, Séamus Fanning, Roger Stephan, Carol Iversen, Felix Reich, Günter Klein, Angelika Lehner, Ben D Tall
Background: Malonate utilization, an important differential trait, well recognized as being possessed by six of the seven Cronobacter species is thought to be largely absent in Cronobacter sakazakii (Csak). The current study provides experimental evidence that confirms the presence of a malonate utilization operon in 24 strains of sequence type (ST) 64, obtained from Europe, Middle East, China, and USA; it offers explanations regarding the genomic diversity and phylogenetic relatedness among these strains, and that of other C...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29515658/chlortetracycline-and-florfenicol-induce-expression-of-genes-associated-with-pathogenicity-in-multidrug-resistant-salmonella-enterica-serovar-typhimurium
#6
Devin B Holman, Shawn M D Bearson, Bradley L Bearson, Brian W Brunelle
Background: Multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium ( S . Typhimurium) is a serious public health threat as infections caused by these strains are more difficult and expensive to treat. Livestock serve as a reservoir for MDR Salmonella , and the antibiotics chlortetracycline and florfenicol are frequently administrated to food-producing animals to treat and prevent various diseases. Therefore, we evaluated the response of MDR S . Typhimurium after exposure to these two antibiotics...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29515657/towards-ending-viral-hepatitis-as-a-public-health-threat-translating-new-momentum-into-concrete-results-in-south-east-asia
#7
Poonam Khetrapal Singh
No abstract text is available yet for this article.
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29483945/molecular-epidemiology-of-genogroup-ii-norovirus-infections-in-acute-gastroenteritis-patients-during-2014-2016-in-pudong-new-area-shanghai-china
#8
Caoyi Xue, Lifeng Pan, Weiping Zhu, Yuanping Wang, Huiqin Fu, Chang Cui, Lan Lu, Sun Qiao, Biao Xu
Background: Norovirus (NoV), a member of the Caliciviridae, is now recognized as the leading cause of acute gastroenteritis (AGE) worldwide. Globally, the GII.4 Sydney_2012 variant has predominated in NoV-related AGE since 2012, although the novel variant GII.17 has also been reported as responsible for gastroenteritis outbreaks in East Asia since 2014. This study aimed to disclose the recent genotype patterns of NoV genogroup II (GII) presenting in AGE patients in Pudong New Area of Shanghai through a laboratory-based syndromic surveillance system...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29483944/molecular-detection-of-human-enteric-viruses-circulating-among-children-with-acute-gastroenteritis-in-valencia-venezuela-before-rotavirus-vaccine-implementation
#9
Ana C Alcalá, Kriss Pérez, Ruth Blanco, Rosabel González, Juan E Ludert, Ferdinando Liprandi, Esmeralda Vizzi
Background: The role of rotavirus as main etiologic agent of diarrhea has been well documented worldwide, including in Venezuela. However, information about the prevalence of gastrointestinal viruses such as calicivirus, adenovirus and astrovirus is limited and the contribution of other agents as Aichi virus and klassevirus is largely unknown. To explore the etiological spectrum of diarrhea associated with agents other than rotaviruses, 227 stool samples from children under 5 years old with acute gastroenteritis, collected in Valencia (Venezuela) from 2001 to 2005, and previously tested as rotavirus-negative, were analyzed for caliciviruses, adenoviruses, astroviruses, Aichi viruses, klasseviruses, picobirnaviruses and enteroviruses by specific RT-PCRs...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29483943/complete-genome-sequence-of-lactobacillus-rhamnosus-pen-a-probiotic-component-of-a-medicine-used-in-prevention-of-antibiotic-associated-diarrhoea-in-children
#10
Piotr Jarocki, Marcin Podleśny, Mariusz Krawczyk, Agnieszka Glibowska, Jarosław Pawelec, Elwira Komoń-Janczara, Oleksandr Kholiavskyi, Michał Dworniczak, Zdzisław Targoński
Background: Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. Results: Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29449892/possible-correlation-between-gut-microbiota-and-immunity-among-healthy-middle-aged-and-elderly-people-in-southwest-china
#11
Xi Shen, Junjie Miao, Qun Wan, Shuyue Wang, Ming Li, Fangfang Pu, Guoqing Wang, Wei Qian, Qian Yu, Francesco Marotta, Fang He
Background: The present study was conducted to investigate the possible association between gut microbes and immunity among healthy middle-aged and elderly individuals in southwest China. A total of 148 healthy adults aged ≥ 50 years were divided into two age groups: middle-aged group (50-59 years; n = 67, 54.13 ± 3.32) and elderly group (≥ 60 years; n = 81, 64.70 ± 3.93). Blood samples were collected to measure serum immune and biochemical indices. Gut microbiota compositions of the groups were characterized on the basis of faecal DNA using 16S rRNA gene sequencing...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29416567/involvement-of-gut-microbiome-in-human-health-and-disease-brief-overview-knowledge-gaps-and-research-opportunities
#12
REVIEW
Dachao Liang, Ross Ka-Kit Leung, Wenda Guan, William W Au
The commensal, symbiotic, and pathogenic microbial community which resides inside our body and on our skin (the human microbiome) can perturb host energy metabolism and immunity, and thus significantly influence development of a variety of human diseases. Therefore, the field has attracted unprecedented attention in the last decade. Although a large amount of data has been generated, there are still many unanswered questions and no universal agreements on how microbiome affects human health have been agreed upon...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29387173/genome-sequence-and-comparative-genomic-analysis-of-a-clinically-important-strain-cd11-4-of-janibacter-melonis-isolated-from-celiac-disease-patient
#13
Atul Munish Chander, Rakesh Kochhar, Devinder Kumar Dhawan, Sanjay Kumar Bhadada, Shanmugam Mayilraj
Background: Janibacter melonis and other member of this genus are known to cause bacteremia and serious clinical comorbidities, but there is no study reporting about pathogenicity attributes of J. melonis. Janibacter terrae is known to cause lethal infection. Reporting the genome of J. melonis CD11-4 and comparative genomics with other members of genus has provided some novel insights that can enable us to understand the mechanisms responsible for its pathogenicity in humans. Results: Comparative genomic analysis by Rapid Annotation Server and Technology revealed the presence of similar virulence determinant genes in both J...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29375672/complete-genome-sequence-of-lactobacillus-pentosus-slc13-isolated-from-mustard-pickles-a-potential-probiotic-strain-with-antimicrobial-activity-against-foodborne-pathogenic-microorganisms
#14
Min-Lang Huang, Jing-Yao Huang, Cheng-Yen Kao, Tony J Fang
Background: Lactobacillus pentosus SLC13 is a high exopolysaccharide (EPS)-producing strain with broad-spectrum antimicrobial activity and the ability to grow in simulated gastrointestinal conditions. SLC13 was isolated from mustard pickles in Taiwan for potential probiotic applications. To better understand the molecular base for its antimicrobial activity and high EPS production, entire genome of SLC13 was determined by PacBio SMRT sequencing. Results: L. pentosus SLC13 contains a genome with a 3,520,510-bp chromosome and a 62,498-bp plasmid...
2018: Gut Pathogens
https://www.readbyqxmd.com/read/29299067/a-multicenter-prospective-study-on-the-diagnostic-performance-of-a-new-liquid-rapid-urease-test-for-the-diagnosis-of-helicobacter-pylori-infection
#15
Werner Dolak, Ceren Bilgilier, Alexander Stadlmann, Judith Leiner, Andreas Püspök, Wolfgang Plieschnegger, Franz Siebert, Friedrich Wewalka, Rainer Schöfl, Ursula Huber-Schönauer, Christian Datz, Susanne Biowski-Frotz, Christoph Högenauer, Christiane Schrutka-Kölbl, Athanasios Makristathis, Maximilian Schöniger-Hekele, Christoph Steininger
Background: Helicobacter pylori ( H. pylori ) causes a diversity of gastric diseases. Rapid urease tests (RUT) are well established for the point-of-care, invasive diagnosis of H. pylori infection. The study aimed to evaluate the diagnostic performance of a new liquid RUT, the preOx-HUT, within a prospective cohort of treatment-naïve patients. Methods: The multicenter prospective clinical trial was conducted at nine Austrian centers for gastrointestinal endoscopy...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29270234/colistin-resistant-escherichia-coli-carrying-mcr-1-in-urban-sludge-samples-dhaka-bangladesh
#16
Aminul Islam, Zillur Rahman, Shirajum Monira, Md Anisur Rahman, Andrew Camilli, Christine M George, Niyaz Ahmed, Munirul Alam
Of 48 bacteria belonging to the family Enterobacteriaceae tested from urban sludge samples, one Escherichia coli isolate was resistant to colistin and possessed the resistance marker gene mcr - 1 found for the first time from Bangladesh. The colistin resistant E. coli was multidrug resistant showing resistance to 11 different antibiotics tested.
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29270233/low-distribution-of-genes-encoding-virulence-factors-in-shigella-flexneri-serotypes-1b-clinical-isolates-from-eastern-chinese-populations
#17
Wenting Fan, Huimin Qian, Wenkang Shang, Chen Ying, Xuedi Zhang, Song Cheng, Bing Gu, Ping Ma
Background: The ability of Shigella to invade, colonize, and eventually kill host cells is influenced by many virulence factors. However, there is no analysis of related genes in Jiangsu Province of China so far. Shigella flexneri was collected from 13 cities of Jiangsu Province through the provincial Centers for Disease Control (CDC) for analysis of distribution of major virulence genes ( ipa H, ipa BCD, ial , vir F, vir B, sig A, set 1A, sep A, sat , pic , set 1B and sen ) detected by PCR technology...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29255490/exposure-to-environmental-microbiota-explains-persistent-abdominal-pain-and-irritable-bowel-syndrome-after-a-major-flood
#18
NurFadhilah Yusof, Nurhazwani Hamid, Zheng Feei Ma, Rona Marie Lawenko, Wan Mohd Zahiruddin Wan Mohammad, Deirdre A Collins, Min Tze Liong, Toshitaka Odamaki, Jinzhong Xiao, Yeong Yeh Lee
Background: After an environmental disaster, the affected community is at increased risk for persistent abdominal pain but mechanisms are unclear. Therefore, our study aimed to determine association between abdominal pain and poor water, sanitation and hygiene (WaSH) practices, and if small intestinal bacterial overgrowth (SIBO) and/or gut dysbiosis explain IBS, impaired quality of life (QOL), anxiety and/or depression after a major flood. Results: New onset abdominal pain, IBS based on the Rome III criteria, WaSH practices, QOL, anxiety and/or depression, SIBO (hydrogen breath testing) and stools for metagenomic sequencing were assessed in flood victims...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29255489/recovery-of-salmonella-isolated-from-eggs-and-the-commercial-layer-farms
#19
Mei Long, Hua Yu, Li Chen, Guoyan Wu, Siyue Zhao, Wenwen Deng, Shujuan Chen, Kang Zhou, Shuliang Liu, Li He, Xiaoling Ao, Yubao Yan, Menggen Ma, Hongning Wang, Margaret A Davis, Lisa Jones, Bei Li, Anyun Zhang, Likou Zou
Background: Salmonella is recognized as a common bacterial cause of foodborne diarrheal illness worldwide, and animal or its food products have been the most common vehicles of the Salmonella infections. This study aimed to investigate the distribution of Salmonella in two commercial layer farms and to determine the genetic relatedness between these strains. The Salmonella isolates were serotyped by slide agglutination using commercial antisera and analyzed for genetic relatedness using pulsed-field gel electrophoresis (PFGE)...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/29255488/modulation-of-the-main-porcine-enteric-neuropeptides-by-a-single-low-dose-of-lipopolysaccharide-lps-salmonella-enteritidis
#20
Anita Mikołajczyk, Sławomir Gonkowski, Dagmara Złotkowska
Background: The present research was conducted to investigate the influence of a low, single dose of LPS, which does not result in any clinical symptoms of intoxication on the expression of selected neuropeptides within the intestines of the domestic pig. Methods: This experiment was conducted on immature female pigs of the Pitrain × Duroc breed (n = five per group). Seven days after the intravenous injection of 10 mL saline solution for control animals and 5 μg/kg b...
2017: Gut Pathogens
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