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Open Biochemistry Journal

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https://www.readbyqxmd.com/read/29760814/short-exposures-to-an-extremely-low-frequency-magnetic-field-elf-mf-enhance-protein-but-not-mrna-alkaline-phosphatase-expression-in-human-osteosarcoma-cells
#1
Tania Rescigno, Anna Capasso, Bruno Bisceglia, Mario Felice Tecce
Background: Among electromagnetic fields treatments used in orthopedics, extremely low-frequency magnetic fields (ELF MF) need more detailed information about the molecular mechanisms of their effects and exposure conditions. Objective: Evaluation of the effects of an ELF MF exposure system, recently introduced among current clinical treatments for fracture healing and other bone diseases, on Alkaline Phosphatase (ALP) activity and expression in a human osteosarcoma cell line (SaOS-2), as marker typically associated to osteogenesis and bone tissue regeneration...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29760813/protective-and-restorative-effects-of-nutrients-and-phytochemicals
#2
REVIEW
Tania Rescigno, Mario F Tecce, Anna Capasso
Intoroduction: Dietary intake fundamentally provides reintegration of energy and essential nutrients to human organisms. However, its qualitative and quantitative composition strongly affects individual's health, possibly being either a preventive or a risk factor. It was shown that nutritional status resulting from long-term exposition to specific diet formulations can outstandingly reduce incidences of most common and most important diseases of the developed world, such as cardiovascular and neoplastic diseases...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29760812/effects-of-glucan-and-vitamin-d-supplementation-on-obesity-and-lipid-metabolism-in-diabetic-retinopathy
#3
Martina Závorková, Vaclav Vetvicka, Josef Richter, Vlastimil Kral, Ivana Liehnova, Dobiasova L Rajnohova
Background: Diabetes mellitus is a chronic disease manifested by an increase of blood glucose. Objective: To evaluate the effects of glucan and vitamin D supplementation in patients with diabetic retinopathy. Method: We evaluated the effects of 3-month supplementation with glucan and vitamin D in 54 patients with diabetic retinopathy. We measured levels of vitamin D, cholesterol, HDL cholesterol, and triglycerides. Results: The supplementation strongly decreased the cholesterol levels and improved the levels of HDL cholesterol...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29576811/delineating-potential-transcriptomic-association-with-organochlorine-pesticides-in-the-etiology-of-epithelial-ovarian-cancer
#4
Harendra K Shah, Muzaffer A Bhat, Tusha Sharma, Basu D Banerjee, Kiran Guleria
Background: Recent studies have shown that there is an increased risk of Epithelial Ovarian Cancer (EOC) with Organochlorine Pesticides (OCPs). However, the alteration in the gene expression profile has not been explored so far. The goal of the present study is to understand the probable molecular mechanism of OCPs toxicity towards discovery of dysregulation of signaling pathway associated with differential gene expression and candidate transcriptomic set of markers in the pathophysiology of EOC in OCPs exposed population...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29541256/secretion-of-legumain-increases-in-conditioned-medium-from-dj-1-knockout-cells-and-in-serum-from-dj-1-knockout-mice
#5
Takuya Yamane, Izumi Kato-Ose, Tatsuji Sakamoto, Yoshihisa Nakano
Background: Asparaginyl endopeptidase, also known as legumain (EC 3.4.22.34) shows strong activity in the mouse kidney. Legumain is also highly expressed in tumors. DJ-1/PARK7 is a Parkinson's disease- and cancer-associated protein. DJ-1 is a coactivator of various transcription factors. Recently, we reported that transcription of the legumain gene is regulated by p53 through DJ-1. Methods: We measured the secretion levels of legumain in a conditioned medium of DJ-1 knockout cells and in serum from DJ-1 knockout mice using Western blotting and ELISA...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29456767/biochemical-characterization-of-two-clinically-relevant-human-fumarase-variants-defective-for-oligomerization
#6
REVIEW
Artemisa Bulku, Todd M Weaver, Melanie B Berkmen
Background: Fumarase, a significant enzyme of energy metabolism, catalyzes the reversible hydration of fumarate to L-malate. Mutations in the FH gene, encoding human fumarase, are associated with fumarate hydratase deficiency (FHD) and hereditary leiomyomatosis and renal cell cancer (HLRCC). Fumarase assembles into a homotetramer, with four active sites. Interestingly, residues from three of the four subunits within the homotetramer comprise each active site. Hence, any mutation affecting oligomerization is predicted to disrupt enzyme activity...
2018: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29387270/plasma-l-carnitine-and-l-lysine-concentrations-in-hiv-infected-patients
#7
Evgeny V Butorov
Background: Virus infections are associated with significant alterations in host cells amino acids profiles that support biosynthetic demands necessary for production of viral progeny. Amino acids play an important role in the pathogenesis of all virus-related infections both as basic substrates for protein synthesis and as regulators in many metabolic pathways. Objective: Our aim was to determine the changes in plasma L-carnitine levels and its amino acid precursor (L-lysine) in HIV-infected patients...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29387269/inflammatory-markers-and-plasma-lipids-in-hiv-patients-a-correlation-analysis-study
#8
Rudo Muswe, Olav Oktedalen, Danai T Zhou, Enita Zinyando, Sandra Shawarira-Bote, Babill Stray-Pedersen, Atipa Siziba, Zvenyika A R Gomo
Background: Recent evidence suggests that HIV infection, even with treatment, increases the risk of coronary heart disease (CHD) and that both chronic inflammation and traditional risk factors play key roles in HIV-associated CHD. Subjects and Methods: Patients (N=152), attending Harare HIV clinic, 26% of them male and 82% of them on antiretroviral therapy (ART), were studied. Inflammatory markers comprising of cytokines such as pro-inflammatory tumor necrosis factor-α, (TNF-α), anti-inflammatory interleukin 10, (IL-10) and highly sensitive C reactive protein (hsCRP) together with lipids were assayed using enzyme linked immunosorbent assay (ELISA), immuno-turbidimetric and enzymatic assays, respectively...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29299072/comparative-studies-on-the-lipid-composition-of-blood-plum-haematostaphis-barteri-pulp-and-seed-oils
#9
Matthew Olaleke Aremu, Hashim Ibrahim, Chrysantus Andrew
Background: Exploring under-utilized plant fruits could be of high significance for food security and nutritional requirements, therefore, it can effectively add to the overall improvement of a nation's economy. Blood plum ( Haematostaphis barteri ) is a wild edible vegetable and its fruit contains pulp and oily seed which are edible. Methods: A study was carried out to determine fatty acid profile, phospholipid and phytosterol contents as well as some physicochemical parameters of pulp and seed oils of Haematostaphis barteri (popularly known as blood plum) using standard analytical techniques...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29290831/effects-of-triton-x-100-and-peg-on-the-catalytic-properties-and-thermal-stability-of-lipase-from-candida-rugosa-free-and-immobilized-on-glyoxyl-agarose
#10
Rafael F Perna, Poliana C Tiosso, Letícia M Sgobi, Angélica M S Vieira, Marcelo F Vieira, Paulo W Tardioli, Cleide M F Soares, Gisella M Zanin
Background: Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose prepared in bicarbonate buffer, pH 10. This change of protocol was enough for allowing the preparation of derivatives actives of CRL on glyoxyl-agarose and verifying the effect of this modified procedure on the properties of the immobilized enzyme...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29151984/hepatic-and-aortic-arch-expression-and-serum-levels-of-syndecan-1-in-apoe-mice
#11
Elena I Leonova, Elena S Sadovnikova, Elvira R Shaykhutdinova, Oxana V Galzitskaya, Arkady N Murashev, Alexandr S Solonin
Background: Heparan sulfate proteoglycan (HSPG) syndecan-1 (Sdc1) acts as a receptor for triglyceride-rich lipoproteins (TRLs), growth factors, chemokines and enzymes. Due to the disordered structure, its function is as diverse as its ligands. In this paper, we have analyzed hepatic and aortic arch expression of Sdc1 in ApoE-/- mice and examined their association with biochemical changes in plasma during the atheroma formation. Methods: ApoE knockout (ApoE-/- ) mice as a model of atherosclerosis were used...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28839472/docosahexaenoic-acid-inhibits-vascular-smooth-muscle-cell-proliferation-induced-by-glucose-variability
#12
Kaliyaperumal Rani, Nway Y Aung
BACKGROUND: Vascular Smooth Muscle cells (VSMC) enact crucial roles in early vasculogenesis and sustenance of vascular integrity. However, aberrant proliferation of VSMC followed by migration into the blood vessel wall leads to the formation of vascular lesions accounting for the degeneration and remodelling of vascular basement membrane. In diabetes, hyperglycaemia accelerates VSMC proliferation and contributes to the initiation and progression of atherosclerotic lesions. Recently, acute glucose fluctuations have been implicated in the abnormal VSMC proliferation and complications of diabetic atherosclerosis...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28567123/identification-of-protease-specificity-using-biotin-labeled-substrates
#13
Hiroyuki Yamamoto, Syota Saito, Yoshikazu Sawaguchi, Michio Kimura
BACKGROUND: Proteolysis constitutes a major post-translational modification. For example, proteases regulate the activation or inactivation of various proteins, such as enzymes, growth factors, and peptide hormones. Proteases have substrate specificity, and protease expression regulates the specific and regional activation or inactivation of several functional proteins. METHODS: We demonstrate a novel method for determining protease specificity through the use of MALDI-TOF mass spectrometry with biotin-labeled substrates...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28567122/a-functional-role-for-the-monomethylated-gln-51-and-lys-53-residues-of-the-49ggqtk53-motif-of-el42-from-human-80s-ribosomes
#14
Stéphanie Eustache, Jean-Bernard Créchet, Tahar Bouceba, Jun-Ichi Nakayama, Mayo Tanaka, Mieko Suzuki, Anne Woisard, Pierre Tuffery, Soria Baouz, Codjo Hountondji
BACKGROUND: We have previously demonstrated that the eukaryote-specific ribosomal protein eL42 of the human 80S ribosome contains seven monomethylated residues, among which are the Gln-51 and Lys-53 residues contained in the 47GFGGQTK53 sequence conserved in all eukaryotic 80S ribosomes. This sequence contains the methylated and universally conserved GGQ motif common for all class-1 translation termination factors responsible for stop codon recognition and for triggering the hydrolysis of the P site-bound peptidyl-tRNA...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28553408/identification-of-cysteine-ubiquitylation-sites-on-the-sec23a-protein-of-the-copii-complex-required-for-vesicle-formation-from-the-er
#15
Giuseppina Amodio, Luigi Margarucci, Ornella Moltedo, Agostino Casapullo, Paolo Remondelli
BACKGROUND: COPII is a multiprotein complex that surrounds carrier vesicles budding from the Endoplasmic Reticulum and allows the recruitment of secretory proteins. The Sec23a protein plays a crucial role in the regulation of the dynamics of COPII formation ensuring the proper function of the secretory pathway. OBJECTIVE: Since few evidences suggest that ubiquitylation could have a role in the COPII regulation, the present study was aimed to establish whether the Sec23a component of the vesicular envelope COPII could be ubiquitylated...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28458728/socioeconomic-deprivation-as-measured-by-the-index-of-multiple-deprivation-and-its-association-with-low-sex-hormone-binding-globulin-in-women
#16
Adrian Heald, Ian Laing, David J McLernon, Rachelle Donn, Andrew J Hartland, Anthony A Fryer, Mark Livingston
OBJECTIVE: Sex hormone binding globulin (SHBG) is a marker of insulin resistance. Given established links between BMI and socioeconomic disadvantage, we investigated how SHBG varies by index of multiple deprivation (IMD). RESEARCH DESIGN AND METHODS: Using laboratory data from a Midlands UK population of mixed ethnicity, we examined the relation between blood concentrations of SHBG and IMD in 1160 women aged between 17 and 71 years. Women with a serum SHBG >250 nmol/L were excluded...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27999622/purification-characterization-and-comparison-between-two-new-l-asparaginases-from-bacillus-pg03-and-bacillus-pg04
#17
Mahsa Rahimzadeh, Manijeh Poodat, Sedigheh Javadpour, Fatemeh Izadpanah Qeshmi, Fereshteh Shamsipour
BACKGROUND: L-asparaginase has been used as a chemotherapeutic agent in treatment of lymphoblastic leukemia. In the present investigation, Bacillus sp. PG03 and Bacillus sp. PG04 were studied. METHODS: L- asparaginases were produced using different culture media and were purified using ion exchange chromatography. RESULTS: Maximum productivity was obtained when asparagine was used as the nitrogen source at pH 7 and 48 h after cultivation. New intracellular L-asparaginases showed an apparent molecular weight of 25 kDa and 30 kDa by SDS-PAGE respectively...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27688820/interrelation-of-glycemic-status-and-neuropsychiatric-disturbances-in-farmers-with-organophosphorus-pesticide-toxicity
#18
Farrukh Jamal, Quazi S Haque, Sangram Singh
BACKGROUND: Diverse group of agro-chemicals are indiscriminately sprayed by the farmers for pest control to enhance crop yield. About 25 million agricultural workers in the developing world suffer from at least one episode of poisoning each year, mainly by anticholinesterase- like organophosphates (OPs). OBJECTIVE: The present study was aimed to establish the OP toxicity in 187 occupationally exposed pesticide sprayers of mango plantation in rural Malihabad, Lucknow, in terms of neuro-cognitive impairment, mental health status, clinical symptoms, diabetes, and hematological factors...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27099634/on-correlation-effect-of-the-van-der-waals-and-intramolecular-forces-for-the-nucleotide-chain-metallic-nanoparticles-carbon-nanotube-binding
#19
M A Khusenov, E B Dushanov, Kh T Kholmurodov, M M Zaki, N H Sweilam
BACKGROUND: The tertiary system of nucleotide chain (NC) - gold nanoparticles (NPs) - carbon nanotube (CNT) represents a great interest in the modern research and application of the bio-nano-technologies. The application aspects include, for example, the development of electronic mobile diagnostic facilities, nanorobotic design for a drug delivery inside living cell, and so on. The small NC chain represents an important stage in the understanding of the interaction mechanism of a full DNA or RNA molecule with NP and CNT...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27073582/are-molecular-vibration-patterns-of-cell-structural-elements-used-for-intracellular-signalling
#20
Werner Jaross
BACKGROUND: To date the manner in which information reaches the nucleus on that part within the three-dimensional structure where specific restorative processes of structural components of the cell are required is unknown. The soluble signalling molecules generated in the course of destructive and restorative processes communicate only as needed. HYPOTHESIS: All molecules show temperature-dependent molecular vibration creating a radiation in the infrared region...
2016: Open Biochemistry Journal
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