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Open Biochemistry Journal

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https://www.readbyqxmd.com/read/29299072/comparative-studies-on-the-lipid-composition-of-blood-plum-haematostaphis-barteri-pulp-and-seed-oils
#1
Matthew Olaleke Aremu, Hashim Ibrahim, Chrysantus Andrew
Background: Exploring under-utilized plant fruits could be of high significance for food security and nutritional requirements, therefore, it can effectively add to the overall improvement of a nation's economy. Blood plum (Haematostaphis barteri) is a wild edible vegetable and its fruit contains pulp and oily seed which are edible. Methods: A study was carried out to determine fatty acid profile, phospholipid and phytosterol contents as well as some physicochemical parameters of pulp and seed oils of Haematostaphis barteri (popularly known as blood plum) using standard analytical techniques...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29290831/effects-of-triton-x-100-and-peg-on-the-catalytic-properties-and-thermal-stability-of-lipase-from-candida-rugosa-free-and-immobilized-on-glyoxyl-agarose
#2
Rafael F Perna, Poliana C Tiosso, Letícia M Sgobi, Angélica M S Vieira, Marcelo F Vieira, Paulo W Tardioli, Cleide M F Soares, Gisella M Zanin
Background: Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose prepared in bicarbonate buffer, pH 10. This change of protocol was enough for allowing the preparation of derivatives actives of CRL on glyoxyl-agarose and verifying the effect of this modified procedure on the properties of the immobilized enzyme...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/29151984/hepatic-and-aortic-arch-expression-and-serum-levels-of-syndecan-1-in-apoe-mice
#3
Elena I Leonova, Elena S Sadovnikova, Elvira R Shaykhutdinova, Oxana V Galzitskaya, Arkady N Murashev, Alexandr S Solonin
Background: Heparan sulfate proteoglycan (HSPG) syndecan-1 (Sdc1) acts as a receptor for triglyceride-rich lipoproteins (TRLs), growth factors, chemokines and enzymes. Due to the disordered structure, its function is as diverse as its ligands. In this paper, we have analyzed hepatic and aortic arch expression of Sdc1 in ApoE(-/-) mice and examined their association with biochemical changes in plasma during the atheroma formation. Methods: ApoE knockout (ApoE(-/-)) mice as a model of atherosclerosis were used...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28839472/docosahexaenoic-acid-inhibits-vascular-smooth-muscle-cell-proliferation-induced-by-glucose-variability
#4
Kaliyaperumal Rani, Nway Y Aung
BACKGROUND: Vascular Smooth Muscle cells (VSMC) enact crucial roles in early vasculogenesis and sustenance of vascular integrity. However, aberrant proliferation of VSMC followed by migration into the blood vessel wall leads to the formation of vascular lesions accounting for the degeneration and remodelling of vascular basement membrane. In diabetes, hyperglycaemia accelerates VSMC proliferation and contributes to the initiation and progression of atherosclerotic lesions. Recently, acute glucose fluctuations have been implicated in the abnormal VSMC proliferation and complications of diabetic atherosclerosis...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28567123/identification-of-protease-specificity-using-biotin-labeled-substrates
#5
Hiroyuki Yamamoto, Syota Saito, Yoshikazu Sawaguchi, Michio Kimura
BACKGROUND: Proteolysis constitutes a major post-translational modification. For example, proteases regulate the activation or inactivation of various proteins, such as enzymes, growth factors, and peptide hormones. Proteases have substrate specificity, and protease expression regulates the specific and regional activation or inactivation of several functional proteins. METHODS: We demonstrate a novel method for determining protease specificity through the use of MALDI-TOF mass spectrometry with biotin-labeled substrates...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28567122/a-functional-role-for-the-monomethylated-gln-51-and-lys-53-residues-of-the-49ggqtk53-motif-of-el42-from-human-80s-ribosomes
#6
Stéphanie Eustache, Jean-Bernard Créchet, Tahar Bouceba, Jun-Ichi Nakayama, Mayo Tanaka, Mieko Suzuki, Anne Woisard, Pierre Tuffery, Soria Baouz, Codjo Hountondji
BACKGROUND: We have previously demonstrated that the eukaryote-specific ribosomal protein eL42 of the human 80S ribosome contains seven monomethylated residues, among which are the Gln-51 and Lys-53 residues contained in the 47GFGGQTK53 sequence conserved in all eukaryotic 80S ribosomes. This sequence contains the methylated and universally conserved GGQ motif common for all class-1 translation termination factors responsible for stop codon recognition and for triggering the hydrolysis of the P site-bound peptidyl-tRNA...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28553408/identification-of-cysteine-ubiquitylation-sites-on-the-sec23a-protein-of-the-copii-complex-required-for-vesicle-formation-from-the-er
#7
Giuseppina Amodio, Luigi Margarucci, Ornella Moltedo, Agostino Casapullo, Paolo Remondelli
BACKGROUND: COPII is a multiprotein complex that surrounds carrier vesicles budding from the Endoplasmic Reticulum and allows the recruitment of secretory proteins. The Sec23a protein plays a crucial role in the regulation of the dynamics of COPII formation ensuring the proper function of the secretory pathway. OBJECTIVE: Since few evidences suggest that ubiquitylation could have a role in the COPII regulation, the present study was aimed to establish whether the Sec23a component of the vesicular envelope COPII could be ubiquitylated...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/28458728/socioeconomic-deprivation-as-measured-by-the-index-of-multiple-deprivation-and-its-association-with-low-sex-hormone-binding-globulin-in-women
#8
Adrian Heald, Ian Laing, David J McLernon, Rachelle Donn, Andrew J Hartland, Anthony A Fryer, Mark Livingston
OBJECTIVE: Sex hormone binding globulin (SHBG) is a marker of insulin resistance. Given established links between BMI and socioeconomic disadvantage, we investigated how SHBG varies by index of multiple deprivation (IMD). RESEARCH DESIGN AND METHODS: Using laboratory data from a Midlands UK population of mixed ethnicity, we examined the relation between blood concentrations of SHBG and IMD in 1160 women aged between 17 and 71 years. Women with a serum SHBG >250 nmol/L were excluded...
2017: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27999622/purification-characterization-and-comparison-between-two-new-l-asparaginases-from-bacillus-pg03-and-bacillus-pg04
#9
Mahsa Rahimzadeh, Manijeh Poodat, Sedigheh Javadpour, Fatemeh Izadpanah Qeshmi, Fereshteh Shamsipour
BACKGROUND: L-asparaginase has been used as a chemotherapeutic agent in treatment of lymphoblastic leukemia. In the present investigation, Bacillus sp. PG03 and Bacillus sp. PG04 were studied. METHODS: L- asparaginases were produced using different culture media and were purified using ion exchange chromatography. RESULTS: Maximum productivity was obtained when asparagine was used as the nitrogen source at pH 7 and 48 h after cultivation. New intracellular L-asparaginases showed an apparent molecular weight of 25 kDa and 30 kDa by SDS-PAGE respectively...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27688820/interrelation-of-glycemic-status-and-neuropsychiatric-disturbances-in-farmers-with-organophosphorus-pesticide-toxicity
#10
Farrukh Jamal, Quazi S Haque, Sangram Singh
BACKGROUND: Diverse group of agro-chemicals are indiscriminately sprayed by the farmers for pest control to enhance crop yield. About 25 million agricultural workers in the developing world suffer from at least one episode of poisoning each year, mainly by anticholinesterase- like organophosphates (OPs). OBJECTIVE: The present study was aimed to establish the OP toxicity in 187 occupationally exposed pesticide sprayers of mango plantation in rural Malihabad, Lucknow, in terms of neuro-cognitive impairment, mental health status, clinical symptoms, diabetes, and hematological factors...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27099634/on-correlation-effect-of-the-van-der-waals-and-intramolecular-forces-for-the-nucleotide-chain-metallic-nanoparticles-carbon-nanotube-binding
#11
M A Khusenov, E B Dushanov, Kh T Kholmurodov, M M Zaki, N H Sweilam
BACKGROUND: The tertiary system of nucleotide chain (NC) - gold nanoparticles (NPs) - carbon nanotube (CNT) represents a great interest in the modern research and application of the bio-nano-technologies. The application aspects include, for example, the development of electronic mobile diagnostic facilities, nanorobotic design for a drug delivery inside living cell, and so on. The small NC chain represents an important stage in the understanding of the interaction mechanism of a full DNA or RNA molecule with NP and CNT...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27073582/are-molecular-vibration-patterns-of-cell-structural-elements-used-for-intracellular-signalling
#12
Werner Jaross
BACKGROUND: To date the manner in which information reaches the nucleus on that part within the three-dimensional structure where specific restorative processes of structural components of the cell are required is unknown. The soluble signalling molecules generated in the course of destructive and restorative processes communicate only as needed. HYPOTHESIS: All molecules show temperature-dependent molecular vibration creating a radiation in the infrared region...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/27053964/available-instruments-for-analyzing-molecular-dynamics-trajectories
#13
I V Likhachev, N K Balabaev, O V Galzitskaya
Molecular dynamics trajectories are the result of molecular dynamics simulations. Trajectories are sequential snapshots of simulated molecular system which represents atomic coordinates at specific time periods. Based on the definition, in a text format trajectory files are characterized by their simplicity and uselessness. To obtain information from such files, special programs and information processing techniques are applied: from molecular dynamics animation to finding characteristics along the trajectory (versus time)...
2016: Open Biochemistry Journal
https://www.readbyqxmd.com/read/26464591/self-organisation-thermotropic-and-lyotropic-properties-of-glycolipids-related-to-their-biological-implications
#14
Patrick Garidel, Yani Kaconis, Lena Heinbockel, Matthias Wulf, Sven Gerber, Ariane Munk, Volkmar Vill, Klaus Brandenburg
Glycolipids are amphiphilic molecules which bear an oligo- or polysaccharide as hydrophilic head group and hydrocarbon chains in varying numbers and lengths as hydrophobic part. They play an important role in life science as well as in material science. Their biological and physiological functions are quite diverse, ranging from mediators of cell-cell recognition processes, constituents of membrane domains or as membrane-forming units. Glycolipids form an exceptional class of liquid-crystal mesophases due to the fact that their self-organisation obeys more complex rules as compared to classical monophilic liquid-crystals...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/26464590/the-optimization-of-soluble-pten-expression-in-escherichia-coli
#15
Yamei Hu, Yang An, Na Fang, Yanzhang Li, Haiying Jin, Adil Nazarali, Shaoping Ji
As a vital tumor suppressor, PTEN (Phosphatase and tension homolog deleted on chromosome 10) is involved in inherited syndromes, and is among the most frequently inactivated tumor suppressor gene in sporadic cancers. PTEN loss-of-function widely occurs in human cancers via a variety of mechanisms, including genetic alterations and posttranslational modification. These suggest PTEN has a role of functional importance in a variety of cancers. In the present study, we constructed a prokaryotic expression vector that efficiently expresses GST-PTEN (the target protein in which PTEN is fused with glutathione S-transferase tag) in E...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/26464589/genetic-polymorphism-of-cytochrome-p450-2c9-enzyme-in-iranian-baluch-ethnic-group
#16
Mojdeh Ghiyas Tabari, Fatemeh Naseri, Maryam Agh Ataby, Abdoljalal Marjani
The aim of the present study is to assess and compare the frequencies of the cytochrome P450 CYP2C9 variations in the Baluch ethnic group (n=110) with other ethnic groups. The allele frequencies of CYP2C9*1, CYP2C9*2 and CYP2C9*3 were 80.90%, 11.82% and 7.27%, respectively. 70.90%, 11.82%, 8.18%, 4.55%, 2.73% and 1.82% of subjects were with CYP2C9*1/*1, CYP2C9*1/*2, CYP2C9*1/*3, CYP2C9*2/*2, CYP2C9*2/*3 and CYP2C9*3/*3 genotypes, respectively. Different mutants may effect on prediction of drug dose requirements in different ethnic groups...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/26157519/prostate-specific-antigen-psa-and-prostate-volume-better-predictor-of-prostate-cancer-for-bosnian-and-herzegovina-men
#17
Jozo Coric, Jasminka Mujic, Elma Kucukalic, Daria Ler
BACKGROUND: The serum prostate specific antigen for the early detection and screening for prostate cancer are very common used among physicians as the best screening tool for prostate cancer. The result of prostate specific antigen levels discriminates whether or not a prostate biopsy should be performed. The lack of specificity is a limitation of PSA as tumor marker, increased PSA concentrations are found not only in patients with prostate cancer but also in patients with benign prostatic disease...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/25972993/phosphorylation-on-trpv4-serine-824-regulates-interaction-with-stim1
#18
Sung H Shin, Eun J Lee, Jaesun Chun, Sunghee Hyun, Sang S Kang
The TRPV4 cation channel, a member of the TRP vanilloid subfamily, is expressed in a broad range of tissues where it participates in the generation of a Ca2+ signal and/or depolarization of membrane potential. Here, we identified stromal interaction molecule 1 precursor (STIM1) as an auxiliary protein of this epithelial Ca2+channel using confocal microscopy analysis and GST pull-down assay. The STIM1 protein associates specifically with the C-terminal tail of TRPV4 to form a complex. In previous reports, we demonstrated that the serine824 residue of TRPV4 is one of the target phosphorylation sites of serum/glucocorticoid regulated kinase 1 (SGK1)...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/25926894/biochemical-and-in-silico-studies-on-pectin-methylesterase-from-g9-variety-of-musa-acuminata-for-delayed-ripening
#19
Charu Verma, Singh R K, Ram B Singh, Sanjay Mishra
Ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. There are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. Some important cell wall degrading enzymes are Polygalacturonase (PG), Pectin methylesterase (PME), Pectin lyase, RGase. PME is known to act as a cell wall hydrolyzing enzyme, responsible for demethyl esterification of cell wall polygalacturonan...
2015: Open Biochemistry Journal
https://www.readbyqxmd.com/read/25741391/evaluation-of-hemoglobin-concentration-in-pregnancy-and-correlation-with-different-altitude-a-study-from-balochistan-plateau-of-pakistan
#20
Zubaida Umar, Mahmood Rasool, Muhammad Asif, Sajjad Karim, Arif Malik, Gohar Mushtaq, Mohammad A Kamal, Arsala Mansoor
BACKGROUND: Anemia refers to a condition having low hemoglobin concentration. Anemia is considered a major risk factor for unfavorable pregnancy outcomes. This is the first study describing the pattern of hemoglobin concentration during pregnancy and its relationship to areas of high and low altitudes in Balochistan (the largest of Pakistan's four provinces). The main objective of this study was to observe hemoglobin levels and prevalence of anemia among pregnant women living in the high or low altitude areas of Balochistan...
2015: Open Biochemistry Journal
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