Ravi Bharadwaj, Madison V Anonick, Swati Jaiswal, Siavash Mashayekh, Ashley Brown, Kimberly A Wodzanowski, Kendi Okuda, Neal Silverman, Catherine L Grimes
NOD1 and NOD2 sense small bacterial peptidoglycan fragments, often called muropeptides, that access the cytosol. These muropeptides include iE-DAP and MDP, the minimal agonists for NOD1 and NOD2, respectively. Here, we synthesized and validated alkyne-modified muropeptides, iE-DAP-Alk and MDP-Alk, for use in click-chemistry reactions. While it has long been known that many cell types respond to extracellular exposure to muropeptides, it is unclear how these innate immune activators access their cytosolic innate immune receptors, NOD1 and NOD2...
October 13, 2023: Innate Immunity