Matthew R Aronson, Amrita Mehta, Ryan M Friedman, Daniel D Ghaderi, Ryan C Borek, Hoang C B Nguyen, Kendra S McDaid, Ian N Jacobs, Natasha Mirza, Riccardo Gottardi
INTRODUCTION: Pediatric subglottic stenosis (SGS) results from prolonged intubation where scar tissue leads to airway narrowing that requires invasive surgery. We have recently discovered that modulating the laryngotracheal microbiome can prevent SGS. Herein, we show how our patent-pending antimicrobial peptide-eluting endotracheal tube (AMP-ET) effectively modulates the local airway microbiota resulting in reduced inflammation and stenosis resolution. MATERIALS AND METHODS: We fabricated mouse-sized ETs coated with a polymeric AMP-eluting layer, quantified AMP release over 10 days, and validated bactericidal activity for both planktonic and biofilm-resident bacteria against Staphylococcus aureus and Pseudomonas aeruginosa ...
August 2023: Cellular and Molecular Bioengineering