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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28699384/preclinical-safety-evaluation-of-allogeneic-ips-cell-based-therapy-in-a-swine-model-of-myocardial-infarction
#1
Carolina Gálvez-Montón, Carolina Soler-Botija, Oriol Iborra-Egea, Idoia Diaz-Guemes, Mercè Martí, Olalla Iglesias-Garcia, Cristina Prat-Vidal, Verónica Crisóstomo, Aida Llucià-Valldeperas, Isaac Perea-Gil, Santiago Roura, Francisco Miguel Sanchez Margallo, Ángel Raya, Antoni Bayés-Genís
The combination of biomatrices and induced pluripotent stem cell (iPSC) derivatives to aid repair and myocardial scar formation may soon become a reality for cardiac regenerative medicine. However, the tumor risk associated to residual undifferentiated cells remains an important safety concern of iPSC-based therapies. This concern is not satisfactorily addressed in xenotransplantation, which require immune suppression of the transplanted animal. Here, we assessed the safety of transplanting undifferentiated iPSC in an allogeneic setting...
July 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28683653/optimization-of-human-myocardium-decellularization-method-for-the-construction-of-implantable-patches
#2
Franca Di Meglio, Daria Nurzynska, Veronica Romano, Rita Miraglia, Immacolata Belviso, Anna Maria Sacco, Valeria Barbato, Mariagrazia Di Gennaro, Giuseppina Granato, Ciro Maiello, Stefania Montagnani, Clotilde Castaldo
Cardiac tissue engineering by the mean of synthetic or natural scaffolds combined with stem/progenitor cells is emerging as the response to the unsatisfactory outcome of approaches based solely on the injection of cells. Parenchymal and supporting cells are surrounded, in vivo, by specialized and tissue-specific microenvironment, consisting mainly of extracellular matrix (ECM) and soluble factors incorporated in the ECM. Since the naturally occurring extracellular matrix is the ideal platform for ensuring cell engraftment, survival, proliferation, and differentiation, the acellular native ECM appears by far the most promising and appealing substrate among all biomaterials tested so far...
July 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28683651/arthroscopic-airbrush-assisted-cell-spraying-for-cartilage-repair-design-development-and-characterization-of-custom-made-arthroscopic-spray-nozzles
#3
Koen Dijkstra, Jan Hendriks, Marcel Karperien, Lucienne A Vonk, Daniel B Saris
INTRODUCTION: Airbrush-assisted cell spraying would facilitate fully arthroscopic filling of cartilage defects, thereby providing a minimally invasive procedure for cartilage repair. This study provides the development and characterisation of custom-made spray nozzles that could serve as a foundation for the development of a BioAirbrush, a platform technology for the arthroscopic application of (cell-laden) hydrogels. METHODS: Custom-made spray nozzles were designed and produced with 3D printing technology...
July 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28548559/the-yucatan-minipig-temporomandibular-joint-disc-structure-function-relationships-support-its-suitability-for-human-comparative-studies
#4
Natalia Vapniarsky, Ashkan Aryaei, Boaz Arzi, David C Hatcher, Jerry C Hu, Kyriacos A Athanasiou
Frequent involvement of the disc in temporomandibular joint (TMJ) disorders warrants attempts to tissue engineer TMJ disc replacements. Physiologically, a great degree of similarity is seen between humans and farm pigs (FPs), but the pig's rapid growth confers a significant challenge for in vivo experiments. Minipigs have a slower growth rate and are smaller than FPs, but minipig TMJ discs have yet to be fully characterized. The objective of this study was to determine the suitability of the minipig for TMJ studies by extensive structural and functional characterization...
July 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28594312/warning-about-the-use-of-critical-size-defects-for-the-translational-study-of-bone-repair-analysis-of-a-sheep-tibial-model
#5
Johan Lammens, Marina Maréchal, Lisbet Geris, Joshua Van der Aa, Hadewych Van Hauwermeiren, Frank P Luyten, Hendrik Delport
The repair of large long bone defects requires complex surgical procedures as the bone loss cannot simply be replaced by autologous grafts due to an insufficient bone stock of the human body. Tissue engineering strategies and the use of Advanced Therapy Medicinal Products (ATMPs) for these reconstructions remain a considerable challenge, in particular since robust outcomes in well-defined large animal models are lacking. To be suitable as a model for treatment of human sized bone defects, we developed a large animal model in both skeletally immature and mature sheep and made close observations on the spontaneous healing of defects...
July 3, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28665236/formation-of-adipose-stromal-vascular-fraction-cell-laden-spheroids-using-a-3d-bioprinter-and-superhydrophobic-surfaces
#6
Brian Gettler, Joseph Zakhari, Piyani Gandhi, Stuart K Williams
The therapeutic infusion of adipose-derived stromal vascular fraction cells (SVF) for the treatment of multiple diseases, has progressed to numerous human clinical trials; however, the often poor retention of the cells following implantation remains a common drawback of direct cell injection. One solution to cellular retention at the injection site has been the use of biogels to encapsulate cells within a microenvironment prior to and upon implantation. The current study utilized 3D bioprinting technology to evaluate the ability to form SVF laden spheroids with collagen I as a gel forming biomatrix...
June 30, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-eptfe-graft-sodding
#7
Stuart K Williams, Marvin E Morris, Paul Kosnik, Kevin D Lye, Gary Gentzkow, Charles B Ross, Amit J Dwivedi, Leigh B Kleinert
Adipose-derived stromal vascular fraction cell populations are being evaluated for numerous clinical applications. The current study evaluated a point of care (POC) technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived stromal vascular fraction cells to be used in the fabrication of a tissue engineered vascular graft in the operating room. A total of 7 patients were enrolled in this FDA registered Investigational Device Exemption clinical trial and received femoral to tibial expanded polytetrafluoroethylene (ePTFE) bypass grafts to treat peripheral arterial disease...
June 28, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28653858/transplantation-of-a-human-tissue-engineered-bowel-in-an-athymic-rat-model
#8
Elie Zakhem, Riccardo Tamburrini, Giuseppe Orlando, Kenneth L Koch, Khalil N Bitar
Intestinal failure is a serious clinical condition characterized by loss of motility, absorptive function, and malnutrition. Current treatments do not provide the optimal solution for patients due to the numerous resulting complications. A bioengineered bowel that contains the necessary cellular components provides a viable option for patients. In this study, human tissue-engineered bowel (hTEB) was developed using a technique, whereby human-sourced smooth muscle cells were aligned and neoinnervated using human-sourced neural progenitor cells, resulting in the formation of intrinsically innervated smooth muscle sheets...
June 27, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28562232/optical-method-to-quantify-mechanical-contraction-and-calcium-transients-of-human-pluripotent-stem-cell-derived-cardiomyocytes
#9
Katrina J Hansen, John T Favreau, Joshua R Gershlak, Michael A Laflamme, Dirk R Albrecht, Glenn R Gaudette
Differentiation of human pluripotent stem cells into cardiomyocytes (hPS-CMs) holds promise for myocardial regeneration therapies, drug discovery, and models of cardiac disease. Potential cardiotoxicities may affect hPS-CM mechanical contraction independent of calcium signaling. Herein, a method using an image capture system is described to measure hPS-CM contractility and intracellular calcium concurrently, with high spatial and temporal resolution. The image capture system rapidly alternates between brightfield and epifluorescent illumination of contracting cells...
June 27, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28537490/the-magnetic-field-of-magnetic-resonance-imaging-systems-does-not-affect-cells-labeled-with-micrometer-sized-iron-oxide-particles
#10
Martin Kluge, Annekatrin Leder, Karl H Hillebrandt, Benjamin Struecker, Dominik Geisel, Timm Denecke, Rebeka D Major, Anja Reutzel-Selke, Peter Tang, Steffen Lippert, Christian Schmidt, Johann Pratschke, Igor M Sauer, Nathanael Raschzok
INTRODUCTION: Labeling using iron oxide particles enables cell tracking through magnetic resonance imaging (MRI). However, the magnetic field can affect the particle-labeled cells. Here, we investigated the effects of a clinical MRI system on primary human hepatocytes labeled using micrometer-sized iron oxide particles (MPIOs). METHODS: HuH7 tumor cells were incubated with increasing concentrations of biocompatible, silica-based, micrometer-sized iron oxide-containing particles (sMPIOs; 40-160 particles/cell)...
June 23, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28637383/inflammation-induced-osteogenesis-in-a-rabbit-tibia-model
#11
Michiel Croes, Willemijn Boot, Moyo C Kruyt, Harrie Weinans, Behdad Pouran, Yvonne van der Helm, Debby Gawlitta, Charles Vogely, Jacqueline Alblas, Wouter Dhert, F Cumhur Oner
Pathologic conditions associated with bone formation can serve as models to identify bone-promoting mediators. The inflammatory response to bacterial infections generally leads to osteolysis and impaired bone healing, but paradoxically, it can also have pro-osteogenic effects. As a potential model to investigate pro-osteogenic stimuli, this study characterizes the bone formation in an established rabbit tibia model of periprosthetic infection. Our hypothesis was that the infection with Staphylococcus aureus (S...
June 22, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28514938/characterization-and-standardization-of-cultured-cardiac-fibroblasts-for-ex-vivo-models-of-heart-fibrosis-and-heart-ischemia
#12
Yue Zhou, Arthur Mark Richards, Peipei Wang
A full understanding of cardiac fibroblast (cFB) biology is essential to study the adverse cardiac remodeling and recovery of myocardium infarction. However, compared to cardiac myocytes, cFBs are less well characterized. Important questions, including the variability introduced by cell age (neonatal vs. adult), culture conditions (passage, plate coating, and culture medium), and responses to stimuli (e.g., hypoxia and drug treatments), have not been well addressed and standardization of techniques is lacking...
June 22, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28622089/large-animal-models-of-meniscus-repair-and-regeneration-a-systematic-review-of-the-state-of-the-field
#13
Sonia Bansal, Niobra M Keah, Alexander L Neuwirth, Olivia O'Reilly, Feini Qu, Breanna Seiber, Sai Mandalapu, Robert Leon Mauck, Miltiadis Zgonis
Injury to the meniscus is common but few viable strategies exist for its repair or regeneration. To address this, animal models have been developed to translate new treatment strategies towards the clinic. However, there is not yet a regulatory document guiding such studies. The purpose of this study was to carry out a systematic review of the literature on meniscus treatment methods and outcomes to define the state of the field. Public databases were queried using search terms related to animal models and meniscus injury and/or repair over the years 1980-2015...
June 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28622076/human-ips-derived-endothelial-cells-for-3d-microphysiological-systems
#14
Yosuke K Kurokawa, Rose T Yin, Michael R Shang, Venktesh S Shirure, Monica L Moya, Steven C George
Microphysiological systems, or "organ-on-a-chip", platforms, aim to recapitulate in vivo physiology using small-scale in vitro tissue models of human physiology. While significant efforts have been made to create vascularized tissues, most reports utilize primary endothelial cells which hinder reproducibility. Here we report the use of human induced pluripotent stem cell-derived endothelial cells (iPS-EC) in developing 3D microvascular networks. We established a CDH5-mCherry reporter iPS cell line, which expresses the vascular endothelial (VE)-cadherin fused to mCherry...
June 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28605991/-i-in-vivo-i-assessment-of-engineered-skin-cell-delivery-with-multimodal-optical-microscopy
#15
Andrew J Bower, Ziad Mahmassani, Youbo Zhao, Eric J Chaney, Marina Marjanovic, Min Kyung Lee, Benedikt Graf, Michael De Lisio, Hyun Joon Kong, Marni D Boppart, Stephen A Boppart
The healing process is often significantly impaired under conditions of chronic or large area wounds, which are often treated clinically using autologous split-thickness skin grafts. However, in many cases, harvesting of donor tissue presents a serious problem such as in the case of very large area burns. In response to this, engineered biomaterials have emerged that attempt to mimic the natural skin environment or deliver a suitable therapy to assist in the healing process. In this study, a custom-built multimodal optical microscope capable of noninvasive structural and functional imaging is used to investigate both the engineered tissue microenvironment and the <i>in vivo</i> wound healing process...
June 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28605989/sinus-floor-augmentation-comparing-an-in-situ-hardening-biphasic-calcium-phosphate-ha-%C3%AE-tcp-bone-graft-substitute-with-a-particulate-biphasic-calcium-phosphate-ha-%C3%AE-tcp-bone-graft-substitute-an-experimental-study-in-sheep
#16
Angelika Wildburger, Vladimir Bubalo, Marton Magyar, Heiner Nagursky, Norbert Jakse, Rainer Schmelzeisen, Sebastian Sauerbier
OBJECTIVE: The aim of the present split-mouth study in sheep was to assess the influence of in situ hardening properties of a biphasic calcium phosphate bone graft substitute (ratio HA/β-TCP = 60/40) compared to a particulate bone graft substitute with the same biphasic core-granule composition without in situ hardening properties on sinus floor augmentation. MATERIAL AND METHODS: Therefore, bilateral sinus floor augmentation was performed in eight sheep. PLGA coated, in situ hardening biphasic bone graft substitute (PLGA-NMP-BCP) was placed into the test site and a particulate biphasic bone graft substitute without PLGA coating (BCP) was used for the contralateral site as a control...
June 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602151/assessment-of-human-acellular-dermis-graft-in-porcine-models-for-ventral-hernia-repair
#17
Sambit Sahoo, Andrew Ryan Baker, Ivy N Haskins, David M Krpata, Michael J Rosen, Kathleen Derwin
OBJECTIVE: Preclinical evaluation of hernia meshes is commonly performed in porcine models. We recently developed two surgically-induced porcine hernia models - an incisional and an excisional model - that formed persistent hernias in the absence of graft repair. Herein, we investigate if these models will continue to form a hernia after graft repair. MATERIALS AND METHODS: Ten pigs were used to create two hernia models - a 10 cm, partial-thickness, incisional defect (n=5) and an 8x8 cm full-thickness, excisional defect (n=5)...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602135/development-of-an-effective-cell-seeding-technique-simulation-implementation-and-analysis-of-contributing-factors
#18
Naser Nasrollahzadeh, Lee Laurent-Applegate, Dominique P Pioletti
Cell seeding in a biomaterial is an important process for tissue engineering applications. It helps to modulate tissue formation or to control initial conditions for mechanobiological studies. The compression release-induced suction (CRIS) seeding technique leads to active infiltration of the cell suspension toward the central region of the scaffold. Its effectiveness, however, may significantly vary depending on several controlling factors such as the rate of loading and unloading or scaffold architecture...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602130/a-novel-method-for-the-activation-of-platelet-rich-plasma-via-bead-mill-homogenizer-for-mesenchymal-stem-cell-culture
#19
Hyunwook Myung, Hyosun Jang, Jae Kyung Myung, Min-Jung Kim, Seung Bum Lee, Won-Suk Jang, Sun-Joo Lee, Hwi-Yool Kim, Seung-Sook Lee, Sehwan Shim, Sunhoo Park
Activated platelet-rich plasma (PRP) has been studied as a replacement for fetal bovine serum (FBS) in stem cell culture. However, current methods are time-consuming or require addition of exogenous substances to activate PRP, which have disadvantages in clinical applications. In this study, we developed a new method for PRP activation using a bead mill homogenizer and compared it with previous methods of PRP activation. PRP was prepared via a two-step centrifugation process and activated via calcium (Ca-PRP), freeze-thaw cycles (FT-PRP), or bead mill homogenizer processing (BM-PRP)...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28578597/optimization-of-methods-for-articular-cartilage-surface-tissue-engineering-cell-density-and-tgf-b-are-critical-self-assembly-and-lubricin-secretion
#20
Kenjiro Iwasa, A Hari Reddi
Objective; Lubricin/Superficial zone protein (SZP)/PRG4 plays an important role in boundary lubrication in articular cartilage. Lubricin is secreted by superficial zone chondrocytes. The specific objective of this investigation is to optimize the methods for tissue engineering of articular cartilage surface. The aim of this study is to investigate the effect of cell density on the self-assembly of superficial zone chondrocytes and attendant lubricin secretion as a functional assessment. Design; Superficial zone chondrocytes were cultivated as a monolayer at low, medium and high densities...
June 3, 2017: Tissue Engineering. Part C, Methods
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