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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28726559/supercritical-carbon-dioxide-assisted-decellularization-of-aorta-and-cornea
#1
Selcan Guler, Bahar Aslan, Pezhman Hosseinian, Halil Murat Aydin
Tissue engineering approaches utilize both natural and synthetic materials in the repair and regeneration processes. A naturally sourced material for this purpose is required to be free from any antigenic matter such as cells or cellular components. Decellularization of tissues may be achieved through chemical or physical removal agents. Supercritical carbon dioxide (sc-CO2) has been used on the purpose of removing bioburden from tissues and offers an alternative to the traditionally used treatment methods...
August 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28810820/quantitative-label-free-evaluation-of-tissue-engineered-skeletal-muscle-through-multiphoton-microscopy
#2
Brian C Syverud, Mary-Ann Mycek, Lisa Marie Larkin
The lack of tools for assessing engineered tissue viability and function in a non-invasive manner is a major regulatory and translational challenge facing tissue engineers. Label-free, nonlinear optical molecular imaging (OMI) has utilized endogenous nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) fluorescence to indicate metabolic activity. Similarly, second harmonic generation (SHG) signals from myosin and collagen can measure overall muscle structural integrity and function...
August 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#3
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
August 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28805140/a-rat-model-for-the-in-vivo-assessment-of-biological-and-tissue-engineered-valvular-and-vascular-grafts
#4
Yukiharu Sugimura, Anna Kathrin Schmidt, Artur Lichtenberg, Alexander Assmann, Payam Akhyari
The demand for an improvement of the biocompatibility and durability of vascular and valvular implants requires translational animal models to study the in vivo fate of cardiovascular grafts. In the present article, a review on the development and application of a microsurgical rat model of infrarenal implantation of aortic grafts and aortic valved conduits is provided. By refinement of surgical techniques and inclusion of hemodynamic considerations, a functional model has been created, which provides a modular platform for the in vivo assessment of biological and tissue-engineered grafts...
August 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28805136/the-ovine-model-for-meniscus-tissue-engineering-considerations-of-anatomy-function-implantation-and-evaluation
#5
Andrzej Brzezinski, Salim A Ghodbane, Jay M Patel, Barbara A Perry, Charles J Gatt, Michael G Dunn
Meniscus injuries represent one of the most-common intra-articular knee injuries. The current treatment options include meniscectomy and allograft transplantation, both with poor long-term outcomes. Therefore, there is a need for regenerative techniques to restore meniscal function. In order to pre-clinically test scaffolds for meniscus replacement, large-animal models need to be established and standardized. This review establishes the anatomical and compositional similarities between the human and sheep menisci and provides guidance for implantation and evaluation of such devices...
August 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28795641/fixation-of-hydrogel-constructs-for-cartilage-repair-in-the-equine-model-a-challenging-issue
#6
Irina A D Mancini, Rafael Angel Vindas Bolanos, Harold Brommer, Miguel Castilho, Alexandro Ribeiro, J P A M van Loon, Anneloes Mensinga, Mattie H P Van Rijen, Jos Malda, René van Weeren
<u><b>Objective</b></u> To evaluate the use of commercial and autologous fibrin glue and of an alternative method based on a 3D-printed polycaprolactone (PCL) anchor for the fixation of hydrogel-based scaffolds in an equine model for cartilage repair. <u><b>Methods</b></u> In a first study, three different hydrogel-based materials were orthotopically implanted in nine horses for 1-4 weeks in 6mm diameter full thickness cartilage defects in the medial femoral trochlear ridge and fixated with commercially available fibrin glue (CFG)...
August 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28795622/animal-models-for-periodontal-tissue-engineering-a-knowledge-generating-process
#7
Karim Mohamed Fawzy El-Sayed, Christof Doerfer
The human periodontium is a uniquely complex vital structure, supporting and anchoring the teeth in their alveolar sockets, playing thereby a decisive role in tooth homeostasis and function. Chronic periodontitis is a highly prevalent immune-inflammatory disease of the periodontium, affecting 15% of adult individuals and is characterized by progressive destruction of the periodontal tooth-investing tissues, culminating in their irreversible damage. Current periodontal evidence-based treatment strategies achieve periodontal healing via repair processes, mostly combating the inflammatory component of the disease, to halt or reduce prospective periodontal tissue loss...
August 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28622089/large-animal-models-of-meniscus-repair-and-regeneration-a-systematic-review-of-the-state-of-the-field
#8
Sonia Bansal, Niobra M Keah, Alexander L Neuwirth, Olivia O'Reilly, Feini Qu, Breanna N Seiber, Sai Mandalapu, Robert L Mauck, Miltiadis H Zgonis
Injury to the meniscus is common, but few viable strategies exist for its repair or regeneration. To address this, animal models have been developed to translate new treatment strategies toward the clinic. However, there is not yet a regulatory document guiding such studies. The purpose of this study was to carry out a systematic review of the literature on meniscus treatment methods and outcomes to define the state of the field. Public databases were queried by using search terms related to animal models and meniscus injury and/or repair over the years 1980-2015...
August 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602151/assessment-of-human-acellular-dermis-graft-in-porcine-models-for-ventral-hernia-repair
#9
Sambit Sahoo, Andrew R Baker, Ivy N Haskins, David M Krpata, Michael J Rosen, Kathleen A Derwin
OBJECTIVE: Preclinical evaluation of hernia meshes is commonly performed in porcine models. We recently developed two surgically induced porcine hernia models-an incisional and an excisional model-that formed persistent hernias in the absence of graft repair. Herein, we investigate if these models will continue to form a hernia after graft repair. MATERIALS AND METHODS: Ten pigs were used to create two hernia models-a 10-cm partial-thickness incisional defect (n = 5) and an 8 × 8-cm full-thickness excisional defect (n = 5)...
August 3, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762895/encapsulation-of-equine-ecfcs-in-highly-uniform-injectable-hydrogel-microspheres-for-local-cell-delivery
#10
Wen J Seeto, Yuan Tian, Randolph L Winter, Fred J Caldwell, Anne A Wooldridge, Elizabeth Ann Lipke
A common challenge in cell therapy is the inability to routinely maintain survival and localization of injected therapeutic cells. Delivering cells by direct injection increases the flexibility of clinical applications, but may cause low cell viability and retention rates due to the high shear force by the needle and mechanical wash out. In this study, we present a custom-built microfluidic device that is capable of rapidly encapsulating high concentrations (10 million cells per mL) of endothelial colony forming cells (ECFCs) in poly(ethylene glycol)-fibrinogen (PF) hydrogel microspheres; resulting cell-laden microspheres are highly uniform in shape and size...
August 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762883/a-paradigm-for-the-evaluation-of-tissue-engineering-biomaterials-and-templates
#11
David Franklyn Williams
Procedures for the evaluation of tissue engineering processes, including those used for the testing of the relevant biomaterials, have not been developed in a logical manner. This perspectives paper discusses the limitations of testing regimes and recommends a very different approach. The main emphasis is on the existing methods for assessing the biological safety of these biomaterials which, it is suggested, are irrelevant for evaluating materials that are intended to facilitate the generation of new tissue...
August 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762881/porcine-ischemic-wound-healing-model-for-preclinical-testing-of-degradable-biomaterials
#12
Prarthana Patil, John R Martin, Samantha M Sarett, Alonda C Pollins, Nancy L Cardwell, Jeffrey M Davidson, Scott Guelcher, Lillian Nanney, Craig Duvall
Impaired wound healing that mimics chronic human skin pathologies is difficult to achieve in current animal models, hindering testing and development of new therapeutic biomaterials that promote wound healing. Here we describe a refinement and simplification of the porcine ischemic wound model that increases the size and number of experimental sites per animal. By comparing three flap geometries, we adopted a superior configuration (15 cm x 10 cm) that enabled testing of twenty 1 cm<sup>2</sup> square wounds in each animal: 8 total ischemic wounds within 4 bipedicle flaps and 12 non-ischemic wounds...
August 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762869/endocultivation-of-scaffolds-with-rhbmp-2-and-vegf165-in-the-omentum-majus-in-a-rabbit-model
#13
Falk Birkenfeld, Andre Sengebusch, Chiara Voelschow, Hendrik Naujokat, Björn Möller, Henning Wieker, Joerg Wiltfang
The reconstruction of defects in the mandible are still challenging. Despite several adequate microvascular bone reconstruction techniques there is a need for ectopic bone endocultivation without drawbacks by donor site morbidity. The omentum majus is described as a good vascularized fleece with undifferentiated cells with potential for bone culturing. In the omentum majus of six rabbits two hydroxyapatite blocks were incorporated for 12 weeks each. The blocks were prepared with rhBMP-2 or VEGF165+rhBMP-2 and wrapped into the omentum...
August 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756760/spray-delivery-of-intestinal-organoids-to-reconstitute-epithelium-on-decellularized-native-extracellular-matrix
#14
Dana M Schwartz, Meryem O Pehlivaner Kara, Allan Goldstein, Harold C Ott, Adam Ekenseair
The native extracellular matrix (ECM) serves as a unique platform for tissue engineering because it provides an organ-specific scaffold in terms of both matrix composition and tissue architecture. However, efficacious cell-seeding techniques for recellularizing the ECM constructs with appropriate cell types to restore biologic function remain under development. In this study, the impact of spraying as a seeding technique for repopulation of decellularized small intestine was investigated. In a series of experiments, CaCo-2 cells was first used to investigate the effect of spray device type and pressure on cell viability and to optimize parameters for seeding intestinal epithelial cells...
July 29, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756748/the-new-zealand-white-rabbit-as-a-model-for-preclinical-studies-addressing-tissue-repair-at-the-level-of-the-abdominal-wall
#15
Juan M Bellón, Marta Rodríguez, Barbara Perez-Kohler, Paloma Pérez-López, Gemma Pascual
In this report, we review the use of the New Zealand White rabbit as the experimental animal for several models of abdominal wall repair. For the repair of an abdominal wall defect such as a hernia in clinical practice, multiple types of prosthetic material exist. Before their marketing, each of these biomaterials needs to be tested in a preclinical setting to confirm its biocompatibility and appropriate behavior at the different tissue interfaces. For preclinical trials, we have always used the New Zealand White rabbit as the model owing to its ease of handling and suitable size...
July 29, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756735/ethical-issues-in-the-use-of-animal-models-for-tissue-engineering-reflections-on-legal-aspects-moral-theory-3rs-strategies-and-harm-benefit-analysis
#16
Gabriel Romero Liguori, Bertus Filippus Jeronimus, Tácia Tavares de Aquinas Liguori, Luiz Felipe Pinho Moreira, Martin C Harmsen
Animal experimentation requires a solid and rational moral foundation. Objective and emphatic decision making and protocol evaluation by researchers and ethics committees remains a difficult and sensitive matter. The present paper presents three perspectives that facilitate a consideration of the minimally acceptable standard for animal experiments, in particular in Tissue Engineering and Regenerative Medicine. Firstly, we review the boundaries provided by law and public opinion in America and Europe. Secondly, we review contemporary moral theory to introduce the Neo-Rawlsian contractarian theory to objectively evaluate the ethics of animal experiments...
July 29, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28750575/creation-and-evaluation-of-new-porcine-model-for-investigation-of-treatments-of-surgical-site-infection
#17
Mahsa Mohiti-Asli, Marije Risselada, Megan Jacob, Behnam Pourdeyhimi, Elizabeth Loboa
Surgical site infection (SSI) is the most common cause of surgical failure, increasing the risks of postoperative mortality and morbidity. Recently, it has been reported that the use of antimicrobial dressings at the incision site help with prevention of SSI. Despite the increased body of research on the development of different types of antimicrobial dressings for this application, to our knowledge nobody has reported a reliable large animal model to evaluate the efficacy of developed materials in a preclinical SSI model...
July 27, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28747146/age-dependent-subchondral-bone-remodeling-and-cartilage-repair-in-a-minipig-defect-model
#18
Christian G Pfeifer, Matthew B Fisher, Vishal Saxena, Minwook Kim, Elizabeth A Henning, David R Steinberg, George R Dodge, Robert Leon Mauck
Following cartilage injury and repair, the subchondral bone plate remodels. Skeletal maturity likely impacts both bone remodeling and inherent cartilage repair capacity. The objective of this study was to evaluate subchondral bone remodeling as a function of injury type, repair scenario and skeletal maturity in a Yucatan minipig model. Cartilage defects (4mm) were created bilaterally in the trochlear groove. Treatment conditions included a full thickness chondral defect (FCD, n=3adult/3juvenile), a partial thickness (~50%) chondral defect (PCD, n=3/3), and FCD treated with microfracture (MFX, n=3/3)...
July 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28747099/in-vivo-models-for-the-evaluation-of-the-osteogenic-potency-of-bone-substitutes-seeded-with-mesenchymal-stem-cells-of-human-origin-a-concise-review-special-issue-animal-models-in-tissue-engineering
#19
Fabian Westhauser, Anne-Sophie Senger, Bruno Reible, Arash Moghaddam
Research concerning bone substitutes is one of the most challenging fields in orthopedic research and has a high clinical relevance, especially since the currently available bone substitutes are limited in their osteostimulative capabilities. In-vitro models for the evaluation of the properties of bone substitutes allow the use of human mesenchymal stem cells (hMSC) seeded onto scaffolds, but suffer from the lack of a physiological environment for those cells. Most in-vivo models include the use of non-human MSC and are therefore lacking in clinical relevance...
July 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28747097/tissue-engineering-strategies-to-improve-osteogenesis-in-the-juvenile-swine-alveolar-cleft-model
#20
Montserrat Caballero, Donna C Jones, Zhengyuan Shan, Sajjad Soleimani, John A van Aalst
Alveolar <b>(gumline)</b> clefts are the most common congenital bone defect in humans, affecting 1 in 700 live births. Treatment <b>to repair these bony</b> defects relies on autologous, <b>cancellous</b> bone transfer from the iliac crest. This harvest requires a second surgical site <b>with increased </b>surgical time associated with potential complications, <b>while providing only limited cancellous bone</b>. Improvements in treatment protocols that avoid these limitations would be beneficial to patients with clefts and other craniofacial bone defects...
July 26, 2017: Tissue Engineering. Part C, Methods
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