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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28938849/animal-models-in-tissue-engineering-and-regenerative-medicine
#1
Jorge Piedrahita, Koudy Williams
Animal models play a central and pivotal role in tissue engineering. While advances in areas such as 3D printing and bioreactor technologies now permit the in vitro development and testing of complex scaffold/cell composites, in vivo testing remains critical not only for refining methods being developed, but also for the critical efficacy and safety testing required for regulatory approval. Yet choosing the appropriate model for a particular application remains a challenge, as each model has its own strengths and weakness...
September 22, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28805140/a-rat-model-for-the-in-vivo-assessment-of-biological-and-tissue-engineered-valvular-and-vascular-grafts
#2
Yukiharu Sugimura, Anna Kathrin Schmidt, Artur Lichtenberg, Alexander Assmann, Payam Akhyari
The demand for an improvement of the biocompatibility and durability of vascular and valvular implants requires translational animal models to study the in vivo fate of cardiovascular grafts. In the present article, a review on the development and application of a microsurgical rat model of infrarenal implantation of aortic grafts and aortic valved conduits is provided. By refinement of surgical techniques and inclusion of hemodynamic considerations, a functional model has been created, which provides a modular platform for the in vivo assessment of biological and tissue-engineered grafts...
September 20, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28922990/streamlined-system-for-conducting-in-vitro-studies-using-decellularized-kidney-scaffolds
#3
Andrew Reyes Padalhin, Chanmi Park, Byong-Taek Lee
Kidney regeneration is a complex process that can only be studied in vitro at a limited capacity due to the inherent structural and functional complexity of its tissues. Thus, a suitable platform for conducting cellular response and development should be established from decellularized tissues with intact microarchitecture. In this study, a modular streamlined system was developed to allow manageable handling and set-up of in vitro studies using decellularized rat kidneys. The system is composed of commercially available parts that can be reused, interchanged, and re-configured based on the desired experimental stage and process...
September 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28922978/6-year-old-sheep-as-a-clinically-relevant-large-animal-model-for-aortic-valve-replacement-using-tissue-engineered-grafts-based-on-decellularized-allogenic-matrix
#4
Karolina Theodoridis, Igor Tudorache, Serghei Cebotari, Tanja Meyer, Alexandru Calistru, Samir Sarikouch, Cristoph Bara, Axel Haverich, Andres Hilfiker
Tissue-engineered (TE) grafts based on decellularized grafts have shown very promising results in preclinical and clinical studies. However, in animal models valves have either been tested in juvenile models or in the clinically less relevant pulmonary valve position. In this study, we tested the grafts in the aortic valve (AV) position of 6-year old sheep, as geriatric patients in need of an aortic valve substitute due to calcification are the largest patient group benefiting from TE grafts. Decellularized AV (n=4) and decellularized AV additionally re-endothelialized with autologous cells (n=3) were implanted in the aortic valve position of 6-year-old female sheep...
September 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28750575/creation-and-evaluation-of-new-porcine-model-for-investigation-of-treatments-of-surgical-site-infection
#5
Mahsa Mohiti-Asli, Marije Risselada, Megan Jacob, Behnam Pourdeyhimi, Elizabeth G Loboa
Surgical site infection (SSI) is the most common cause of surgical failure, increasing the risks of postoperative mortality and morbidity. Recently, it has been reported that the use of antimicrobial dressings at the incision site help with prevention of SSI. Despite the increased body of research on the development of different types of antimicrobial dressings for this application, to our knowledge, nobody has reported a reliable large animal model to evaluate the efficacy of developed materials in a preclinical SSI model...
September 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28911258/effect-of-cryopreservation-on-cell-laden-hydrogels-comparison-of-different-cryoprotectants
#6
Nicola Cagol, Walter Bonani, Devid Maniglio, Claudio Migliaresi, Antonella Motta
Cell encapsulation in hydrogels is a technique that offers a variety of applications, ranging from drug delivery to biofabrication of three-dimensional scaffolds. The assembly of cell-laden hydrogel building blocks aims to generate complex biological constructs by manipulating microscale units. An important issue for the clinical implementation of this technique is the long-term storage of a large stock of cell/hydrogel building blocks. In this work, the impact of cryopreservation on the viability and functionality of cells encapsulated in alginate matrices is presented comparing different cryoprotective agents (CPAs)...
September 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28741426/influence-of-different-irradiation-protocols-on-vascularization-and-bone-formation-parameters-in-rat-femora
#7
Ulrike Rottensteiner-Brandl, Luitpold Distel, Martin Stumpf, Tobias Fey, Katrin Köhn, Ulf Bertram, Lara F Lingens, Peter Greil, Raymund E Horch, Andreas Arkudas
Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B) and three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT), and real-time polymerase chain reaction...
September 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28895470/generation-of-a-close-to-native-in-vitro-system-to-study-lung-cells-ecm-crosstalk
#8
Zuzana Garlikova, Ana Catarina Silva, Ana Rabata, David Potesil, Ivana Ihnatova, Jana Dumkova, Zuzana Koledova, Zbynek Zdrahal, Vladimir Vinarsky, Ales Hample, Perpetua Pinto-do-O, Diana S Nascimento
Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior. In vitro culture systems are often poor in ECM constituents thus not allowing for naturally occurring cell-ECM interactions. This study reports on a straightforward and efficient method for generation of ECM scaffolds from lung tissue and its subsequent in vitro application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions and DNase...
September 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28877649/the-effect-of-hypothermic-and-cryogenic-preservation-on-engineered-neural-tissue
#9
Adam George Edward Day, Kulraj Singh Bhangra, Celia Murray-Dunning, Lara Stevanato, James Phillips
This study explored different approaches to preserve Engineered Neural Tissue (EngNT), a stabilised cellular collagen hydrogel containing columns of aligned Schwann cells for nervous system repair. The ability to preserve EngNT without disrupting cellular and extracellular components and structures is important for clinical translation and commercialisation. Stabilised cellular gels and EngNT constructs were preserved under various conditions and cell survival assessed using live/dead microscopy and metabolic assay...
September 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28877648/the-chorioallantoic-membrane-cam-assay-for-biomaterial-testing-in-tissue-engineering-a-short-term-in-vivo-preclinical-model
#10
Ines Moreno-Jimenez, Janos M Kanczler, Gry S Hulsart-Billstrom, Stefanie Inglis, Richard O C Oreffo
The fields of regenerative medicine and tissue engineering offer significant promise to address the urgent unmet need for therapeutic strategies in a number of debilitating conditions, diseases and tissue needs of an aging population. Critically, the safety and efficacy of these pioneering strategies needs to be assessed prior to clinical application, often necessitating animal research as a prerequisite. The growing number of newly developed potential treatments, together with the ethical concerns involved in the application of in vivo studies, requires the implementation of alternative models to facilitate such screening of new treatments...
September 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28877641/collagen-and-hydroxyapatite-scaffolds-activate-distinct-osteogenesis-signaling-pathways-in-adult-adipose-derived-multipotent-stromal-cells
#11
Wei Duan, Masudul Haque, Michael T Kearney, Mandi Lopez
Osteogenic cell signaling pathway disruption varies among bone diseases. This investigation was designed to identify adipose derived multipotent stromal cell (ASC) and bone graft scaffold combinations for local, targeted restoration of gene expression and extracellular matrix (ECM) deposition. Human ASC osteogenesis on bone graft materials was quantified following culture in stromal (S), osteogenic (O) or osteogenic for 48 hours followed by stromal medium (OS) to test the two-part hypothesis: 1) Identical ASC isolates on distinct bone graft scaffolds demonstrate unique viability, differentiation, ECM production and gene expression in the same culture conditions; 2) Identical ASC-bone graft scaffold combinations have different cell viability, differentiation, ECM production, and gene expression when cultured in S, O or OS medium...
September 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756735/ethical-issues-in-the-use-of-animal-models-for-tissue-engineering-reflections-on-legal-aspects-moral-theory-three-rs-strategies-and-harm-benefit-analysis
#12
Gabriel R Liguori, Bertus F Jeronimus, Tácia T de Aquinas Liguori, Luiz Felipe P Moreira, Martin C Harmsen
Animal experimentation requires a solid and rational moral foundation. Objective and emphatic decision-making and protocol evaluation by researchers and ethics committees remain a difficult and sensitive matter. This article presents three perspectives that facilitate a consideration of the minimally acceptable standard for animal experiments, in particular, in tissue engineering (TE) and regenerative medicine. First, we review the boundaries provided by law and public opinion in America and Europe. Second, we review contemporary moral theory to introduce the Neo-Rawlsian contractarian theory to objectively evaluate the ethics of animal experiments...
September 5, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28747099/in-vivo-models-for-the-evaluation-of-the-osteogenic-potency-of-bone-substitutes-seeded-with-mesenchymal-stem-cells-of-human-origin-a-concise-review
#13
Fabian Westhauser, Anne-Sophie Senger, Bruno Reible, Arash Moghaddam
Research concerning bone substitutes is one of the most challenging fields in orthopedic research and has a high clinical relevance, especially since the currently available bone substitutes are limited in their osteostimulative capabilities. In vitro models for the evaluation of the properties of bone substitutes allow the use of human mesenchymal stem cells (hMSCs) seeded onto scaffolds, but suffer from the lack of a physiological environment for those cells. Most in vivo models include the use of non-hMSC and are therefore lacking in clinical relevance...
September 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28726574/rise-of-the-pigs-utilization-of-the-porcine-model-to-study-musculoskeletal-biomechanics-and-tissue-engineering-during-skeletal-growth
#14
Stephanie G Cone, Paul B Warren, Matthew B Fisher
Large animal models play an essential role in the study of tissue engineering and regenerative medicine (TERM), as well as biomechanics. The porcine model has been increasingly used to study the musculoskeletal system, including specific joints, such as the knee and temporomandibular joints, and tissues, such as bone, cartilage, and ligaments. In particular, pigs have been utilized to evaluate the role of skeletal growth on the biomechanics and engineered replacements of these joints and tissues. In this review, we explore the publication history of the use of pig models in biomechanics and TERM discuss interspecies comparative studies, highlight studies on the effect of skeletal growth and other biological considerations in the porcine model, and present challenges and emerging opportunities for using this model to study functional TERM...
September 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28844179/noninvasive-absolute-epr-oxygen-imaging-for-the-assessment-of-tissue-graft-oxygenation
#15
Mrignayani Kotecha, Boris Epel, Sriram Ravindran, Deborah Dorcemus, Syam P Nukavarapu, Howard J Halpern
Oxygen is the single most important molecule for sustaining life and therefore an important variable in tissue engineering and regenerative medicine (TERM). It has been shown that the change in oxygen concentration in an artificial or engineered tissue graft affects cell survival, differentiation, and tissue growth in profound ways. However, at present, there are no reliable methods to map partial oxygen pressure in growing artificial tissues. Here, we adapt and test the suitability of electron paramagnetic resonance oxygen imaging (EPROI) in assessing tissue graft oxygenation in vitro...
August 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28826357/3d-printing-and-angiogenesis-tailored-agarose-type-i-collagen-blends-comprise-3d-printability-and-angiogenesis-potential-for-tissue-engineered-substitutes
#16
Franziska Kreimendahl, Marius Köpf, Anja Lena Thiebes, Daniela F Duarte Campos, Andreas Blaeser, Thomas Schmitz-Rode, Christian Apel, Stefan Jockenhoevel, Horst Fischer
3D bioprinting is a promising technology for manufacturing cell-laden tissue engineered constructs. Larger tissue substitutes, however, require a vascularized network to ensure nutrition supply. Therefore, tailored bioinks combining 3D printability and cell-induced vascularization are needed. We hypothesize that tailored hydrogel blends made of agarose-type I collagen and agarose-fibrinogen are 3D printable and will allow the formation of capillary-like structures by human umbilical vein endothelial cells and human dermal fibroblasts...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28826352/pcl-pdms-pcl-copolymer-based-microspheres-mediate-cardiovascular-differentiation-from-embryonic-stem-cells
#17
Liqing Song, Mohammad Faisal Ahmed, Yan Li, Julie Bejoy, Changchun Zeng, Yan Li
Poly-ϵ-caprolactone (PCL) based microspheres have received much attention as drug or growth factor delivery carriers and tissue engineering scaffolds due to their biocompatibility, biodegradability, and tunable biophysical properties. In addition, PCL and polydimethylsiloxane (PDMS) can be fabricated into thermo-responsive shape memory polymers for various biomedical applications (e.g., smart sutures and vascular stents). However, the influence of biophysical properties of PCL-PDMS based microspheres on stem cell lineage commitment has not been well understood...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28825366/calvarial-defects-cell-based-reconstructive-strategies-in-the-murine-model
#18
Matthew P Murphy, Natalina Quarto, Michael T Longaker, Derrick C Wan
Calvarial defects pose a continued clinical dilemma for reconstruction. Advancements within the fields of stem cell biology and tissue engineering have enabled researchers to develop reconstructive strategies using animal models. We review the utility of various animal models and focus on the mouse, which has aided investigators in understanding cranial development and calvarial bone healing. The murine model has also been used to study regenerative approaches to critical-sized calvarial defects, and we discuss the application of stem cells such as bone marrow derived mesenchymal stromal cells, adipose derived stromal cells, muscle derived stem cells, and pluripotent stem cells to address deficient bone in this animal...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28637383/inflammation-induced-osteogenesis-in-a-rabbit-tibia-model
#19
Michiel Croes, Willemijn Boot, Moyo C Kruyt, Harrie Weinans, Behdad Pouran, Yvonne J M van der Helm, Debby Gawlitta, H Charles Vogely, Jacqueline Alblas, Wouter J A Dhert, F Cumhur Öner
Pathologic conditions associated with bone formation can serve as models to identify bone-promoting mediators. The inflammatory response to bacterial infections generally leads to osteolysis and impaired bone healing, but paradoxically, it can also have pro-osteogenic effects. As a potential model to investigate pro-osteogenic stimuli, this study characterizes the bone formation in an established rabbit tibia model of periprosthetic infection. Our hypothesis was that the infection with Staphylococcus aureus (S...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28810820/quantitative-label-free-evaluation-of-tissue-engineered-skeletal-muscle-through-multiphoton-microscopy
#20
Brian C Syverud, Mary-Ann Mycek, Lisa Marie Larkin
The lack of tools for assessing engineered tissue viability and function in a non-invasive manner is a major regulatory and translational challenge facing tissue engineers. Label-free, nonlinear optical molecular imaging (OMI) has utilized endogenous nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) fluorescence to indicate metabolic activity. Similarly, second harmonic generation (SHG) signals from myosin and collagen can measure overall muscle structural integrity and function...
August 16, 2017: Tissue Engineering. Part C, Methods
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