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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28314378/novel-cryopreservation-method-for-the-effective-collection-of-dental-pulp-stem-cells
#1
Yusuke Takebe, Seiko Tatehara, Tatsuhiro Fukushima, Reiko Tokuyama-Toda, Rika Yasuhara, Kenji Mishima, Kazuhito Satomura
Dental pulp stem cells (DPSCs) are an attractive cell source for use in cell-based therapy, regenerative medicine, and tissue engineering because DPSCs have high cell proliferation ability and multi-differentiation capacity. However, several problems are associated with the collection and preservation of DPSCs for future cell-based therapy. In addition, the isolation of DPSCs for cryopreservation is time-consuming and expensive. In this study, we developed a novel cryopreservation method for dental pulp tissues that can isolate suitable DPSCs after thawing cryopreserved tissue...
March 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28177263/isolation-of-adipose-derived-stromal-vascular-fraction-cells-using-a-novel-point-of-care-device-cell-characterization-and-review-of-the-literature
#2
James Christian Brown, Hulan Shang, Ying Li, Ning Yang, Nikita Patel, Adam J Katz
The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the clinical point of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived hSVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, Gram stain, and culture...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28142371/preparation-and-characterization-of-a-chitosan-gelatin-extracellular-matrix-scaffold-and-its-application-in-tissue-engineering
#3
Xiaoyan Wang, Tailong Yu, Guanghua Chen, Jilong Zou, Jianzhong Li, Jinglong Yan
Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. However, adipose-derived ECM (A-dECM) has never been applied in bone tissue engineering, and it is unknown whether it would be beneficial to the growth of bone marrow mesenchymal stem cells (BMSCs). In this study, we produced chitosan/gelatin/A-dECM (C/G/A-dECM) scaffolds via lyophilization and crosslinking; chitosan/gelatin (C/G) scaffolds were used as controls. For the C/G/A-dECM scaffolds, the average pore size was 285...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28142337/cell-gel-mechanical-interactions-as-an-approach-to-rapidly-and-quantitatively-reveal-invasive-subpopulations-of-metastatic-cancer-cells
#4
Martha B Alvarez-Elizondo, Daphne Weihs
We present a novel mechanobiology-based invasiveness assay to rapidly and quantitatively evaluate the mechanical invasiveness of metastatic cancer cells and identify invasive subpopulations, without need for chemoattractants and independent of serum content. A commonly accepted assay to determine metastatic potential in vitro is the Boyden chamber assay, where the percentage of serum-starved cells that can long-term transmigrate/invade through subcell size membrane pores is quantified; those experiments typically take 2-3 days and require serum-starvation...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28106517/traditional-invasive-and-synchrotron-based-noninvasive-assessments-of-three-dimensional-printed-hybrid-cartilage-constructs-in-situ
#5
Adeola D Olubamiji, Ning Zhu, Tuanjie Chang, Chijioke K Nwankwo, Zohreh Izadifar, Ali Honaramooz, Xiongbiao Chen, B Frank Eames
Three-dimensional (3D)-printed constructs made of polycaprolactone and chondrocyte-impregnated alginate hydrogel (hybrid cartilage constructs) can mimic the biphasic nature of articular cartilage, thus offering promise for cartilage tissue engineering applications. Notably, the regulatory pathway for medical device development requires validation of such constructs through in vitro bench tests and in vivo preclinical examinations for premarket approval. For this, noninvasive imaging techniques are required for effective evaluation of the progress of these cartilage constructs, especially when implanted in animal models or human subjects...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28093047/three-dimensional-cell-printing-of-large-volume-tissues-application-to-ear-regeneration
#6
Jung-Seob Lee, Byoung Soo Kim, Donghwan Seo, Jeong Hun Park, Dong-Woo Cho
The three-dimensional (3D) printing of large-volume cells, printed in a clinically relevant size, is one of the most important challenges in the field of tissue engineering. However, few studies have reported the fabrication of large-volume cell-printed constructs (LCCs). To create LCCs, appropriate fabrication conditions should be established: Factors involved include fabrication time, residence time, and temperature control of the cell-laden hydrogel in the syringe to ensure high cell viability and functionality...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28068869/a-regenerative-cardiac-patch-formed-by-spray-painting-of-biomaterials-onto-the-heart
#7
Junnan Tang, Adam Vandergriff, Zegen Wang, Michael Taylor Hensley, Jhon Cores, Tyler A Allen, Phuong-Uyen Dinh, Jinying Zhang, Thomas George Caranasos, Ke Cheng
Layering a regenerative polymer scaffold on the surface of the heart, termed as a cardiac patch, has been proven to be effective in preserving cardiac function after myocardial infarction (MI). However, the placement of such a patch on the heart usually needs open-chest surgery, which is traumatic, therefore prevents the translation of this strategy into the clinic. We sought to device a way to apply a cardiac patch by spray painting in situ polymerizable biomaterials onto the heart with a minimally invasive procedure...
March 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28081688/focus-ion-beam-scanning-electron-microscopy-characterization-of-osteoclastic-resorption-of-calcium-phosphate-substrates
#8
Anna Diez-Escudero, Montserrat Espanol, Edgar B Montufar, Gemma Di Pompo, Gabriela Ciapetti, Nicola Baldini, Maria-Pau Ginebra
This article presents the application of dual focused ion beam/scanning electron microscopy (FIB-SEM) imaging for preclinical testing of calcium phosphates with osteoclast precursor cells and how this high-resolution imaging technique is able to reveal microstructural changes at a level of detail previously not possible. Calcium phosphate substrates, having similar compositions but different microstructures, were produced using low- and high-temperature processes (biomimetic calcium-deficient hydroxyapatite [CDHA] and stoichiometric sintered hydroxyapatite, respectively)...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28068883/a-quantitative-three-dimensional-image-analysis-tool-for-maximal-acquisition-of-spatial-heterogeneity-data
#9
Mark C Allenby, Ruth Misener, Nicki Panoskaltsis, Athanasios Mantalaris
Three-dimensional (3D) imaging techniques provide spatial insight into environmental and cellular interactions and are implemented in various fields, including tissue engineering, but have been restricted by limited quantification tools that misrepresent or underutilize the cellular phenomena captured. This study develops image postprocessing algorithms pairing complex Euclidean metrics with Monte Carlo simulations to quantitatively assess cell and microenvironment spatial distributions while utilizing, for the first time, the entire 3D image captured...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28068876/tissue-engineered-model-of-human-osteolytic-bone-tumor
#10
Aranzazu Villasante, Alessandro Marturano-Kruik, Samuel T Robinson, Zen Liu, X Edward Guo, Gordana Vunjak-Novakovic
Ewing's sarcoma (ES) is a poorly differentiated pediatric tumor of aggressive behavior characterized by propensity to metastasize to bone. Interactions between the tumor and bone cells orchestrate a vicious cycle in which tumor cells induce osteoclast differentiation and activation to cause osteolytic lesions, broken bones, pain, and hypercalcemia. The lack of controllable models that can recapitulate osteolysis in ES impedes the development of new therapies and limits our understanding of how tumor cells invade bone...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28056722/hyaluronic-acid-coating-enhances-biocompatibility-of-nonwoven-pga-scaffold-and-cartilage-formation
#11
Xunxun Lin, Wenbo Wang, Wenjie Zhang, Zhiyong Zhang, Guangdong Zhou, Yilin Cao, Wei Liu
Synthetic polymers such as polyglycolic acid (PGA) fibers are the traditional tissue engineering scaffolds that are widely used for engineering a variety of soft tissues. However, the major disadvantage of this polymer material is its released acidic degradation products that trigger inflammatory response and fibrotic process, which affects the biocompatibility and the quality of the engineered tissues. In this study, the effect of hyaluronic acid (HA) coating on improving PGA biocompatibility was explored...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28007011/mimicking-the-acute-myeloid-leukemia-niche-for-molecular-study-and-drug-screening
#12
Mohammad Houshmand, Masoud Soleimani, Amir Atashi, Giuseppe Saglio, Mohammad Abdollahi, Mahin Nikougoftar Zarif
Bone marrow niche is a major contributing factor in leukemia development and drug resistance in acute myeloid leukemia (AML) patients. Although mimicking leukemic bone marrow niche relies on two-dimensional (2D) culture conditions, it cannot recapitulate complex bone marrow structure that causes introduction of different three-dimensional (3D) scaffolds. Simultaneously, microfluidic platform by perfusing medium culture mimic interstitial fluid flow, along with 3D scaffold would help for mimicking bone marrow microenvironment...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27981878/xenogeneic-decellularized-scaffold-a-novel-platform-for-ovary-regeneration
#13
Wen-Yue Liu, Shi-Gang Lin, Ru-Yi Zhuo, Yuan-Yuan Xie, Wei Pan, Xian-Feng Lin, Fei-Xia Shen
Women younger than 40 years may face early menopause because of premature ovarian failure (POF). The cause of POF can be idiopathic or iatrogenic, especially the cancer-induced oophorectomy and chemo- or radiation therapy. The current treatments, including hormone replacement therapy (HRT) and cryopreservation techniques, have increased risk of ovarian cancer and may reintroduce malignant cells after autografting. Decellularization technique has been regarded as a novel regenerative medicine strategy for organ replacement, wherein the living cells of an organ are removed, leaving the extracellular matrix (ECM) for cellular seeding...
February 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27968705/efficient-nonviral-transfection-of-primary-intervertebral-disc-cells-by-electroporation-for-tissue-engineering-application
#14
Rahel D May, Adel Tekari, Daniela A Frauchiger, Anna Krismer, Lorin M Benneker, Benjamin Gantenbein
Low back pain (LBP) is an increasing global health problem associated with intervertebral disc (IVD) trauma and degeneration. Current treatment options include surgical interventions with partial unsatisfactory outcomes reported such as failure to relieve LBP, nonunions, nerve injuries, or adjacent segment disease. Cell-based therapy and tissue engineered IVD constructs supplemented with transfected disc cells that incorporate factors enhancing matrix synthesis represent an appealing approach to regenerate the IVD...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27923327/formation-of-nanofibrous-matrices-three-dimensional-scaffolds-and-microspheres-from-theory-to-practice
#15
Chi Ma, Xiaohua Liu
Nanofibrous architecture presents unique biophysical cues to facilitate cellular responses and is considered an indispensable feature of a biomimetic three-dimensional (3D) scaffold and cell carrier. While electrospinning is a widely used method to prepare natural extracellular matrix-like nanofibers, it faces significant challenges to incorporate nanofibrous architecture into well-defined macroporous 3D scaffolds or injectable microspheres. Here we report a nonelectrospinning approach that is effective at generating nanofibers from a variety of synthetic and natural biodegradable polymers and integrating these nanofibers into (1) 3D scaffolds with constructive geometry and designed internal macropore structures; and (2) injectable microspheres...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27923320/three-dimensional-coculture-model-to-analyze-the-cross-talk-between-endothelial-and-smooth-muscle-cells
#16
Minu Karthika Ganesan, Richard Finsterwalder, Heide Leb, Ulrike Resch, Karin Neumüller, Rainer de Martin, Peter Petzelbauer
The response of blood vessels to physiological and pathological stimuli partly depends on the cross talk between endothelial cells (EC) lining the luminal side and smooth muscle cells (SMC) building the inner part of the vascular wall. Thus, the in vitro analysis of the pathophysiology of blood vessels requires coculture systems of EC and SMC. We have developed and validated a modified three-dimensional sandwich coculture (3D SW-CC) of EC and SMC using open μ-Slides with a thin glass bottom allowing direct imaging...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27901409/a-noninvasive-in-vitro-monitoring-system-reporting-skeletal-muscle-differentiation
#17
Deniz Öztürk-Kaloglu, David Hercher, Philipp Heher, Katja Posa-Markaryan, Simon Sperger, Alice Zimmermann, Susanne Wolbank, Heinz Redl, Ara Hacobian
Monitoring of cell differentiation is a crucial aspect of cell-based therapeutic strategies depending on tissue maturation. In this study, we have developed a noninvasive reporter system to trace murine skeletal muscle differentiation. Either a secreted bioluminescent reporter (Metridia luciferase) or a fluorescent reporter (green fluorescent protein [GFP]) was placed under the control of the truncated muscle creatine kinase (MCK) basal promoter enhanced by variable numbers of upstream MCK E-boxes. The engineered pE3MCK vector, coding a triple tandem of E-Boxes and the truncated MCK promoter, showed twentyfold higher levels of luciferase activation compared with a Cytomegalovirus (CMV) promoter...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27875930/hybrid-aorta-constructs-via-in-situ-crosslinking-of-poly-glycerol-sebacate-elastomer-within-a-decellularized-matrix
#18
Selcan Guler, Pezhman Hosseinian, Halil Murat Aydin
Decellularization of tissues and organs has high potential to obtain unique conformation and composition as native tissue structure but may result in weakened tissue mechanical strength. In this study, poly(glycerol-sebacate) (PGS) elastomers were combined with decellularized aorta fragments to investigate the changes in mechanical properties. PGS prepolymer was synthesized via microwave irradiation and then in situ crosslinked within the decellularized aorta extracellular matrix (ECM). Tensile strength (σ) values were found comparable as 0...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27869545/noninvasive-monitoring-of-three-dimensional-chondrogenic-constructs-using-molecular-beacon-nanosensors
#19
Li Min Tay, Christian Wiraja, David C Yeo, Yingnan Wu, Zheng Yang, Yon Jin Chuah, Eng Hin Lee, Yuejun Kang, Chenjie Xu
Chondrogenic differentiation of human mesenchymal stem cells (MSCs) in three-dimensional hydrogel holds promise as a method for repairing injured articular cartilage. Given MSC plasticity (its potential to mature into alternative lineages), nondestructive monitoring is critical for the optimization of chondrogenic differentiation conditions and the evaluation of the final product. However, conventional validation/assessments of the differentiation process (i.e., quantitative reverse transcription polymerase chain reaction [qRT-PCR] and histology) are end-point assays requiring disruption of the sample...
January 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27846787/cryopreservation-of-brain-endothelial-cells-derived-from-human-induced-pluripotent-stem-cells-is-enhanced-by-rho-associated-coiled-coil-containing-kinase-inhibition
#20
Hannah K Wilson, Madeline G Faubion, Michael K Hjortness, Sean P Palecek, Eric V Shusta
The blood-brain barrier (BBB) maintains brain homeostasis but also presents a major obstacle to brain drug delivery. Brain microvascular endothelial cells (BMECs) form the principal barrier and therefore represent the major cellular component of in vitro BBB models. Such models are often used for mechanistic studies of the BBB in health and disease and for drug screening. Recently, human induced pluripotent stem cells (iPSCs) have emerged as a new source for generating BMEC-like cells for use in in vitro human BBB studies...
December 2016: Tissue Engineering. Part C, Methods
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