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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28537490/the-magnetic-field-of-magnetic-resonance-imaging-systems-does-not-affect-cells-labeled-with-micrometer-sized-iron-oxide-particles
#1
Martin Kluge, Annekatrin Leder, Karl H Hillebrandt, Benjamin Struecker, Dominik Geisel, Timm Denecke, Rebeka D Major, Anja Reutzel-Selke, Peter Tang, Steffen Lippert, Christian Schmidt, Johann Pratschke, Igor M Sauer, Nathanael Raschzok
INTRODUCTION: Labeling using iron oxide particles enables cell tracking through magnetic resonance imaging (MRI). However, the magnetic field can affect the particle-labeled cells. Here, we investigated the effects of a clinical MRI system on primary human hepatocytes labeled using micrometer-sized iron oxide particles (MPIOs). METHODS: HuH7 tumor cells were incubated with increasing concentrations of biocompatible, silica-based, micrometer-sized iron oxide-containing particles (sMPIOs; 40-160 particles/cell)...
June 23, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28637383/inflammation-induced-osteogenesis-in-a-rabbit-tibia-model
#2
Michiel Croes, Willemijn Boot, Moyo C Kruyt, Harrie Weinans, Behdad Pouran, Yvonne van der Helm, Debby Gawlitta, Charles Vogely, Jacqueline Alblas, Wouter Dhert, F Cumhur Oner
Pathologic conditions associated with bone formation can serve as models to identify bone-promoting mediators. The inflammatory response to bacterial infections generally leads to osteolysis and impaired bone healing, but paradoxically, it can also have pro-osteogenic effects. As a potential model to investigate pro-osteogenic stimuli, this study characterizes the bone formation in an established rabbit tibia model of periprosthetic infection. Our hypothesis was that the infection with Staphylococcus aureus (S...
June 22, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28622089/large-animal-models-of-meniscus-repair-and-regeneration-a-systematic-review-of-the-state-of-the-field
#3
Sonia Bansal, Niobra M Keah, Alexander L Neuwirth, Olivia O'Reilly, Feini Qu, Breanna Seiber, Sai Mandalapu, Robert Leon Mauck, Miltiadis Zgonis
Injury to the meniscus is common but few viable strategies exist for its repair or regeneration. To address this, animal models have been developed to translate new treatment strategies towards the clinic. However, there is not yet a regulatory document guiding such studies. The purpose of this study was to carry out a systematic review of the literature on meniscus treatment methods and outcomes to define the state of the field. Public databases were queried using search terms related to animal models and meniscus injury and/or repair over the years 1980-2015...
June 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28622076/human-ips-derived-endothelial-cells-for-3d-microphysiological-systems
#4
Yosuke K Kurokawa, Rose T Yin, Michael R Shang, Venktesh S Shirure, Monica L Moya, Steven C George
Microphysiological systems, or "organ-on-a-chip", platforms, aim to recapitulate in vivo physiology using small-scale in vitro tissue models of human physiology. While significant efforts have been made to create vascularized tissues, most reports utilize primary endothelial cells which hinder reproducibility. Here we report the use of human induced pluripotent stem cell-derived endothelial cells (iPS-EC) in developing 3D microvascular networks. We established a CDH5-mCherry reporter iPS cell line, which expresses the vascular endothelial (VE)-cadherin fused to mCherry...
June 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28605991/-i-in-vivo-i-assessment-of-engineered-skin-cell-delivery-with-multimodal-optical-microscopy
#5
Andrew J Bower, Ziad Mahmassani, Youbo Zhao, Eric J Chaney, Marina Marjanovic, Min Kyung Lee, Benedikt Graf, Michael De Lisio, Hyun Joon Kong, Marni D Boppart, Stephen A Boppart
The healing process is often significantly impaired under conditions of chronic or large area wounds, which are often treated clinically using autologous split-thickness skin grafts. However, in many cases, harvesting of donor tissue presents a serious problem such as in the case of very large area burns. In response to this, engineered biomaterials have emerged that attempt to mimic the natural skin environment or deliver a suitable therapy to assist in the healing process. In this study, a custom-built multimodal optical microscope capable of noninvasive structural and functional imaging is used to investigate both the engineered tissue microenvironment and the <i>in vivo</i> wound healing process...
June 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28605989/sinus-floor-augmentation-comparing-an-in-situ-hardening-biphasic-calcium-phosphate-ha-%C3%AE-tcp-bone-graft-substitute-with-a-particulate-biphasic-calcium-phosphate-ha-%C3%AE-tcp-bone-graft-substitute-an-experimental-study-in-sheep
#6
Angelika Wildburger, Vladimir Bubalo, Marton Magyar, Heiner Nagursky, Norbert Jakse, Rainer Schmelzeisen, Sebastian Sauerbier
OBJECTIVE: The aim of the present split-mouth study in sheep was to assess the influence of in situ hardening properties of a biphasic calcium phosphate bone graft substitute (ratio HA/β-TCP = 60/40) compared to a particulate bone graft substitute with the same biphasic core-granule composition without in situ hardening properties on sinus floor augmentation. MATERIAL AND METHODS: Therefore, bilateral sinus floor augmentation was performed in eight sheep. PLGA coated, in situ hardening biphasic bone graft substitute (PLGA-NMP-BCP) was placed into the test site and a particulate biphasic bone graft substitute without PLGA coating (BCP) was used for the contralateral site as a control...
June 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602151/assessment-of-human-acellular-dermis-graft-in-porcine-models-for-ventral-hernia-repair
#7
Sambit Sahoo, Andrew Ryan Baker, Ivy N Haskins, David M Krpata, Michael J Rosen, Kathleen Derwin
OBJECTIVE: Preclinical evaluation of hernia meshes is commonly performed in porcine models. We recently developed two surgically-induced porcine hernia models - an incisional and an excisional model - that formed persistent hernias in the absence of graft repair. Herein, we investigate if these models will continue to form a hernia after graft repair. MATERIALS AND METHODS: Ten pigs were used to create two hernia models - a 10 cm, partial-thickness, incisional defect (n=5) and an 8x8 cm full-thickness, excisional defect (n=5)...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602135/development-of-an-effective-cell-seeding-technique-simulation-implementation-and-analysis-of-contributing-factors
#8
Naser Nasrollahzadeh, Lee Laurent-Applegate, Dominique P Pioletti
Cell seeding in a biomaterial is an important process for tissue engineering applications. It helps to modulate tissue formation or to control initial conditions for mechanobiological studies. The compression release-induced suction (CRIS) seeding technique leads to active infiltration of the cell suspension toward the central region of the scaffold. Its effectiveness, however, may significantly vary depending on several controlling factors such as the rate of loading and unloading or scaffold architecture...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28602130/a-novel-method-for-the-activation-of-platelet-rich-plasma-via-bead-mill-homogenizer-for-mesenchymal-stem-cell-culture
#9
Hyunwook Myung, Hyosun Jang, Jae Kyung Myung, Min-Jung Kim, Seung Bum Lee, Won-Suk Jang, Sun-Joo Lee, Hwi-Yool Kim, Seung-Sook Lee, Sehwan Shim, Sunhoo Park
Activated platelet-rich plasma (PRP) has been studied as a replacement for fetal bovine serum (FBS) in stem cell culture. However, current methods are time-consuming or require addition of exogenous substances to activate PRP, which have disadvantages in clinical applications. In this study, we developed a new method for PRP activation using a bead mill homogenizer and compared it with previous methods of PRP activation. PRP was prepared via a two-step centrifugation process and activated via calcium (Ca-PRP), freeze-thaw cycles (FT-PRP), or bead mill homogenizer processing (BM-PRP)...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28594312/warning-about-the-use-of-critical-size-defects-for-the-translational-study-of-bone-repair-analysis-of-a-sheep-tibial-model
#10
Johan Lammens, Marina Maréchal, Liesbet Geris, Joshua Vanderaa, Hadewych Van Hauwermeiren, Frank P Luyten, Hendrik Delport
The repair of large long bone defects requires complex surgical procedures as the bone loss cannot simply be replaced by autologous grafts due to an insufficient bone stock of the human body. Tissue engineering strategies and the use of Advanced Therapy Medicinal Products (ATMPs) for these reconstructions remain a considerable challenge, in particular since robust outcomes in well-defined large animal models are lacking. To be suitable as a model for treatment of human sized bone defects we developed a large animal model in both skeletally immature and mature sheep and made close observations on the spontaneous healing of defects...
June 8, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28578597/optimization-of-methods-for-articular-cartilage-surface-tissue-engineering-cell-density-and-tgf-b-are-critical-self-assembly-and-lubricin-secretion
#11
Kenjiro Iwasa, A Hari Reddi
Objective; Lubricin/Superficial zone protein (SZP)/PRG4 plays an important role in boundary lubrication in articular cartilage. Lubricin is secreted by superficial zone chondrocytes. The specific objective of this investigation is to optimize the methods for tissue engineering of articular cartilage surface. The aim of this study is to investigate the effect of cell density on the self-assembly of superficial zone chondrocytes and attendant lubricin secretion as a functional assessment. Design; Superficial zone chondrocytes were cultivated as a monolayer at low, medium and high densities...
June 3, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28562232/optical-method-to-quantify-mechanical-contraction-and-calcium-transients-of-human-pluripotent-stem-cell-derived-cardiomyocytes
#12
Katrina J Hansen, John T Favreau, Joshua R Gershlak, Michael A Laflamme, Dirk R Albrecht, Glenn R Gaudette
Differentiation of human pluripotent stem cells into cardiomyocytes (hPS-CM) holds promise for myocardial regeneration therapies, drug discovery, and models of cardiac disease. Potential cardiotoxicities may affect hPS-CM mechanical contraction independent of calcium signaling. Herein, a method using an image capture system is described to measure hPS-CM contractility and intracellular calcium concurrently, with high spatial and temporal resolution. The image capture system rapidly alternates between brightfield and epifluorescent illumination of contracting cells...
May 31, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28548559/the-yucatan-minipig-tmj-disc-structure-function-relationships-support-its-suitability-for-human-comparative-studies
#13
Natalia Vapniarsky, Ashkan Aryaei, Boaz Arzi, Jerry C Hu, David C Hatcher, Kyriacos A Athanasiou
Frequent involvement of the disc in temporomandibular joint (TMJ) disorders warrants attempts to tissue engineer TMJ disc replacements. Physiologically, a great degree of similarity is seen between humans and farm pigs (FPs), but the pig's rapid growth confers a significant challenge for in vivo experiments. Minipigs have a slower growth rate and are smaller than FPs, but minipig TMJ discs have yet to be fully characterized. The objective of this study was to determine the suitability of the minipig for TMJ studies by extensive structural and functional characterization...
May 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28537529/a-versatile-protocol-for-studying-calvarial-bone-defect-healing-in-a-mouse-model
#14
Rebekah Margaret Samsonraj, Amel Dudakovic, Pengfei Zan, Oksana Pichurin, Simon Cool, Andre J van Wijnen
Animal models are vital tools for the preclinical development and testing of therapies aimed at providing solutions for several musculoskeletal disorders. For bone tissue engineering strategies addressing non-union conditions, rodent models are particularly useful for studying bone healing in a controlled environment. The mouse calvarial defect model permits evaluation of drug, growth factor or cell transplantation efficacy, together with offering the benefit of utilizing genetic models to study intramembranous bone formation within defect sites...
May 24, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28514938/characterization-and-standardization-of-cultured-cardiac-fibroblasts-for-ex-vivo-models-of-heart-fibrosis-and-heart-ischemia
#15
Yue Zhou, Arthur Mark Richards, Peipei Wang
A full understanding of cardiac fibroblast (cFB) biology is essential to study the adverse cardiac remodeling and recovery of myocardium infarction. However, compared to cardiac myocytes, cFB are less well-characterized. Important questions including the variability introduced by cell age (neonatal vs. adult), culture conditions (passage, plate coating and culture medium), and responses to stimuli (e.g. hypoxia and drug treatments) have not been well addressed and standardization of techniques is lacking. This variability invites inconsistency and the confounding of study conclusions...
May 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28493788/development-of-in-vitro-embryo-production-system-using-collagen-matrix-gel-attached-vascular-endothelial-growth-factor-derived-from-interleukin-1-beta-treated-porcine-endometrial-tissue
#16
Hye-In Han, Sang-Hee Lee, Choon-Keun Park
The aim of this study was to establish an embryo culture system using collagen gel attached with vascular endothelial growth factor (VEGF) derived from interleukin-1 beta (IL-1β)-treated endometrial tissues from pigs. Endometria were separated from the porcine uterus at the follicular phase of the estrous cycle and were cultured with IL-1β. The collagen gels co-incubated with IL-1β-treated endometria (C, without endometrial tissue; CE, with endometrial tissue; and CEI, IL-1β-treated endometrial tissue) were used for embryo culture...
May 11, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471303/bone-marrow-derived-mesenchymal-stem-cells-as-autologous-therapy-in-dogs-with-naturally-occurring-intervertebral-disc-disease-feasibility-safety-and-preliminary-results
#17
Frank Steffen, Lucas Smolders, Anne Roentgen, Alessandro Bertolo, Jivko Stoyanov
The intradiscal application of mesenchymal stem cells (MSC) is considered a promising strategy for intervertebral disc (IVD) regeneration. Although many studies have been published, the feasibility and regenerative effects of intradiscal MSC application has not been evaluated in an animal model suffering from naturally occurring degenerative disc disease. Six German Shepherd Dogs suffering from naturally occurring degenerative IVD disease were included. Autologous MSC were isolated from bone marrow (iliac crest) and cultured for 3 weeks...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28478703/a-bioreactor-to-identify-the-driving-mechanical-stimuli-of-tissue-growth-and-remodeling
#18
Mathieu A J van Kelle, Pim J A Oomen, Jurgen A Bulsink, Marloes W J T Janssen-van den Broek, Richard G P Lopata, Marcel C M Rutten, Sandra Loerakker, Carlijn V C Bouten
Tissue growth and remodeling are essential processes that should ensure long-term functionality of tissue-engineered (TE) constructs. Even though it is widely recognized that these processes strongly depend on mechanical stimuli, the underlying mechanisms of mechanically induced growth and remodeling are only partially understood. It is generally accepted that cells sense mechanical changes and respond by altering their surroundings, by means of extracellular matrix growth and remodeling, in an attempt to return to a certain preferred mechanical homeostatic state...
June 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471326/chondrocyte-cell-sheet-transplantation-for-treating-monoiodoacetate-induced-arthritis-in-rats
#19
Seiju Hayashi, Naosuke Kamei, Yasunari Ikuta, Ryo Shimizu, Masakazu Ishikawa, Nobuo Adachi, Mitsuo Ochi
Engineered cell sheets that are fabricated by using temperature-responsive culture dishes are now available in the clinical setting. Engineered chondrocyte cell sheets are used in clinical trials for treating focal articular cartilage defects. However, the efficacy of engineered chondrocyte cell sheets for treating diffusely degenerated articular cartilage remains unknown. In the present study, we assessed the utility of engineered chondrocyte cell sheets for treating diffusely degenerated articular cartilage in a monoiodoacetate (MIA)-induced arthritis animal model...
June 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471308/fabrication-of-orientation-controlled-3d-tissues-using-a-layer-by-layer-technique-and-3d-printed-a-thermoresponsive-gel-frame
#20
Yoshinari Tsukamoto, Takami Akagi, Fumiaki Shima, Mitsuru Akashi
Herein, we report the fabrication of orientation-controlled tissues similar to heart and nerve tissues using a cell accumulation and three-dimensional (3D) printing technique. We first evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermoresponsive polymer, by using a robotic dispensing 3D printer. HBC polymer could be laminated to a height of 1124 ± 14 μm. Based on this result, we fabricated 3D gel frames of various shapes, such as square, triangular, rectangular, and circular, for shape control of 3D tissue and then normal human cardiac fibroblasts (NHCFs) coated with extracellular matrix nanofilms were seeded in the frames...
June 2017: Tissue Engineering. Part C, Methods
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