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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/28514938/characterization-and-standardization-of-cultured-cardiac-fibroblasts-for-ex-vivo-models-of-heart-fibrosis-and-heart-ischemia
#1
Yue Zhou, Arthur Mark Richards, Peipei Wang
A full understanding of cardiac fibroblast (cFB) biology is essential to study the adverse cardiac remodeling and recovery of myocardium infarction. However, compared to cardiac myocytes, cFB are less well-characterized. Important questions including the variability introduced by cell age (neonatal vs. adult), culture conditions (passage, plate coating and culture medium), and responses to stimuli (e.g. hypoxia and drug treatments) have not been well addressed and standardization of techniques is lacking. This variability invites inconsistency and the confounding of study conclusions...
May 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28493788/development-of-in-vitro-embryo-production-system-using-collagen-matrix-gel-attached-vascular-endothelial-growth-factor-derived-from-interleukin-1-beta-treated-porcine-endometrial-tissue
#2
Hye-In Han, Sang-Hee Lee, Choon-Keun Park
The aim of this study was to establish an embryo culture system using collagen gel attached with vascular endothelial growth factor (VEGF) derived from interleukin-1 beta (IL-1β)-treated endometrial tissues from pigs. Endometria were separated from the porcine uterus at the follicular phase of the estrous cycle and were cultured with IL-1β. The collagen gels co-incubated with IL-1β-treated endometria (C, without endometrial tissue; CE, with endometrial tissue; and CEI, IL-1β-treated endometrial tissue) were used for embryo culture...
May 11, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28478703/a-bioreactor-to-identify-the-driving-mechanical-stimuli-of-tissue-growth-and-remodeling
#3
Mathieu A J van Kelle, Pim J A Oomen, Jurgen A Bulsink, Marloes W J T Janssen-van den Broek, Richard G P Lopata, Marcel Rutten, Sandra Loerakker, Carlijn Bouten
Tissue growth and remodeling are essential processes that should ensure long-term functionality of tissue engineered (TE) constructs. Even though it is widely recognized that these processes strongly depend on mechanical stimuli, the underlying mechanisms of mechanically-induced growth and remodeling are only partially understood. It is generally accepted that cells sense mechanical changes and respond by altering their surroundings, by means of extracellular matrix (ECM) growth and remodeling, in an attempt to return to a certain preferred mechanical homeostatic state...
May 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471326/chondrocyte-cell-sheet-transplantation-for-treating-monoiodoacetate-induced-arthritis-model-in-rat
#4
Seiju Hayashi, Naosuke Kamei, Yasunari Ikuta, Ryo Shimizu, Masakazu Ishikawa, Nobuo Adachi, Mitsuo Ochi
Engineered cell-sheets fabricated using temperature-responsive culture dishes are now available in the clinical setting. Engineered chondrocyte cell-sheets are used in clinical trials for treating focal articular cartilage defects. However, the efficacy of engineered chondrocyte cell-sheets for treating diffusely degenerated articular cartilage remains unknown. In the present study, we assessed the utility of engineered chondrocyte cell-sheets for treating diffusely degenerated articular cartilage in a monoiodoacetate (MIA)-induced arthritis animal model...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471308/fabrication-of-orientation-controlled-3d-tissues-using-a-layer-by-layer-technique-and-3d-printed-a-thermo-responsive-gel-frame
#5
Yoshinari Tsukamoto, Takami Akagi, Fumiaki Shima, Mitsuru Akashi
Herein, we report the fabrication of orientation-controlled tissues similar to heart and nerve tissues using a cell accumulation and three-dimensional (3D) printing technique. We firstly evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermo-responsive polymer, by using a robotic dispensing 3D printer. HBC polymer could be laminated to a height of 1,124 ± 14 μm. Based on this result, we fabricated 3D gel frames of various shapes such as square, triangular, rectangular and circular for shape control of 3D tissue and then normal human cardiac fibroblasts (NHCFs) coated with extracellular matrix (ECM) nanofilms were seeded in the frames...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471303/bone-marrow-derived-mesenchymal-stem-cells-as-autologous-therapy-in-dogs-with-naturally-occurring-intervertebral-disc-disease-feasibility-safety-and-preliminary-results
#6
Frank Steffen, Lucas Smolders, Anne Roentgen, Alessandro Bertolo, Jivko Stoyanov
The intradiscal application of mesenchymal stem cells (MSC) is considered a promising strategy for intervertebral disc (IVD) regeneration. Although many studies have been published, the feasibility and regenerative effects of intradiscal MSC application has not been evaluated in an animal model suffering from naturally occurring degenerative disc disease. Six German Shepherd Dogs suffering from naturally occurring degenerative IVD disease were included. Autologous MSC were isolated from bone marrow (iliac crest) and cultured for 3 weeks...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471293/in-vitro-experimental-model-for-the-long-term-analysis-of-cellular-dynamics-during-bronchial-tree-development-from-lung-epithelial-cells
#7
Masaya Hagiwara, Naomichi Maruta, Moegi Marumoto
Lung branching morphogenesis has been studied for decades, but the underlying developmental mechanisms are still not fully understood. Cellular movements dynamically change during the branching process, but it is difficult to observe long-term cellular dynamics by in vivo or tissue culture experiments. Therefore, developing an in vitro experimental model of bronchial tree would provide an essential tool for developmental biology, pathology, and systems biology. Here, we succeeded in reconstructing a bronchial tree in vitro by using primary human bronchial epithelial cells...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471271/ecm-hydrogel-derived-from-human-umbilical-cord-as-a-scaffold-for-neural-tissue-repair-and-its-comparison-to-ecm-from-cns-and-non-cns-porcine-tissues
#8
Zuzana Koci, Karel Výborný, Jana Dubišová, Irena Vackova, Aleš Jäger, Oleg Lunov, Klára Jiráková, Sarka Kubinova
Extracellular matrix (ECM) hydrogels prepared by tissue decellularization have been reported as natural injectable materials suitable for neural tissue repair. Here, we prepared ECM hydrogel derived from human umbilical cord (UC) and evaluated its composition, and mechanical and biological properties in comparison to the previously described ECM hydrogels, derived from porcine urinary bladder (UB), brain and spinal cord. The ECM hydrogels did not differ from each other in the concentration of collagen, while the highest content of glycosaminoglycans, as well as the shortest gelation time was found for UC-ECM...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28471270/biomimetic-assembly-of-huvecs-and-muscle-cells-in-microgrooved-collagen-porous-scaffolds
#9
Shangwu Chen, Naoki Kawazoe, Guoping Chen
Porous scaffolds can be used to engineer 3D tissues for tissue repair. Coculture of vascular endothelial cells and the cells of target tissue in porous scaffolds is promising to engineer vascularized tissue. However, it is difficult to induce regeneration of anisotropic tissues such as muscle and nerve that have well aligned cells, blood vessels and extracellular matrix by using conventional porous scaffolds with homogeneous pore structures. In this study, we developed collagen porous scaffolds with parallel and concave microgrooves by using micropatterned ice template and freeze-drying...
May 4, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28504918/sheep-placenta-cotyledons-a-noninvasive-source-of-ovine-mesenchymal-stem-cells
#10
Iris Ribitsch, Souyet Chang-Rodriguez, Monika Egerbacher, Simone Gabner, Sinan Gueltekin, Johann Huber, Therese Schuster, Florien Jenner
Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28457187/laser-etched-designs-for-molding-hydrogel-based-engineered-tissues
#11
Fabiola Munarin, Nicholas J Kaiser, Tae Yun Kim, Bum-Rak Choi, Kareen L K Coulombe
Rapid prototyping and fabrication of elastomeric molds for sterile culture of engineered tissues allow for the development of tissue geometries that can be tailored to different in vitro applications and customized as implantable scaffolds for regenerative medicine. Commercially available molds offer minimal capabilities for adaptation to unique conditions or applications versus those for which they are specifically designed. Here we describe a replica molding method for the design and fabrication of poly(dimethylsiloxane) (PDMS) molds from laser-etched acrylic negative masters with ∼0...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28403748/an-effective-method-of-atelocollagen-type-1-3-isolation-from-human-placenta-and-its-in-vitro-characterization-in-two-dimesional-and-three-dimensional-cell-culture-applications
#12
Johannes Hackethal, Severin Mühleder, Alexandra Hofer, Karl Heinrich Schneider, Johanna Prüller, Simone Hennerbichler, Heinz Redl, Andreas Teuschl
Pepsin-solubilized atelocollagen can be used to form highly complex three-dimensional matrices for a broad spectrum of tissue engineering applications. Moreover, it has a long history as a favorable biomaterial in pharmaceutical and medical industries. So far, the main sources for these approaches are collagens from xenogenic sources. Yet, these nonhuman collagens carry a risk of provoking immune reactions in patients. Here we describe an effective method of isolating atelocollagen type 1/3 (COL1/3) from human placenta...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28401793/developing-a-customized-perfusion-bioreactor-prototype-with-controlled-positional-variability-in-oxygen-partial-pressure-for-bone-and-cartilage-tissue-engineering
#13
Poh Soo Lee, Hagen Eckert, Ricarda Hess, Michael Gelinsky, Derrick Rancourt, Roman Krawetz, Gianaurelio Cuniberti, Dieter Scharnweber
Skeletal development is a multistep process that involves the complex interplay of multiple cell types at different stages of development. Besides biochemical and physical cues, oxygen tension also plays a pivotal role in influencing cell fate during skeletal development. At physiological conditions, bone cells generally reside in a relatively oxygenated environment whereas chondrocytes reside in a hypoxic environment. However, it is technically challenging to achieve such defined, yet diverse oxygen distribution on traditional in vitro cultivation platforms...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28372521/bone-response-to-porous-poly-methyl-methacrylate-cement-loaded-with-hydroxyapatite-particles-in-a-rabbit-mandibular-model
#14
Yue Sa, Na Yu, Joop G C Wolke, Nattharee Chanchareonsook, Bee Tin Goh, Yining Wang, Fang Yang, John A Jansen
The aim of the current study was to evaluate bone formation and tissue response to porous poly(methyl methacrylate) (PMMA) cement with or without hydroxyapatite (HA) in a rabbit mandibular model. Therefore, 14 New Zealand White rabbits were randomly divided into two groups of seven according to the designed study end points of 4 and 12 weeks. For each rabbit, two decorticated defects (6 mm in height and 10 mm in width for each) were prepared at both sides of the mandible. Subsequently, the defects were filled with, respectively, porous PMMA and porous PMMA-HA cement...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28314378/cryopreservation-method-for-the-effective-collection-of-dental-pulp-stem-cells
#15
Yusuke Takebe, Seiko Tatehara, Tatsuhiro Fukushima, Reiko Tokuyama-Toda, Rika Yasuhara, Kenji Mishima, Kazuhito Satomura
Dental pulp stem cells (DPSCs) are an attractive cell source for use in cell-based therapy, regenerative medicine, and tissue engineering because DPSCs have a high cell proliferation ability and multidifferentiation capacity. However, several problems are associated with the collection and preservation of DPSCs for use in future cell-based therapy. In particular, the isolation of DPSCs for cryopreservation is time consuming and expensive. In this study, we developed a novel cryopreservation method (NCM) for dental pulp tissues to isolate suitable DPSCs after thawing cryopreserved tissue...
May 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28406755/a-modified-hydroxyproline-assay-based-on-hydrochloric-acid-in-ehrlich-s-solution-accurately-measures-tissue-collagen-content
#16
Derek D Cissell, Jarrett M Link, Jerry C Hu, Kyriacos A Athanasiou
Collagen quantification has long been relevant to biomedical research and clinical practice to characterize tissues and determine disease states. The hydroxyproline assay, while a broadly employed method of quantifying collagen, uses perchloric acid to dissolve Ehrlich's reagent. Since perchloric acid poses occupational safety hazards and high costs, in this study, a new hydroxyproline assay was developed that replaces perchloric acid with a relatively safer and cheaper alternative, hydrochloric acid (HCl)...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28406754/spheroid-coculture-of-hematopoietic-stem-progenitor-cells-and-monolayer-expanded-mesenchymal-stem-stromal-cells-in-polydimethylsiloxane-microwells-modestly-improves-in-vitro-hematopoietic-stem-progenitor-cell-expansion
#17
Kathryn Futrega, Kerry Atkinson, William B Lott, Michael R Doran
While two-dimensional (2D) monolayers of mesenchymal stem/stromal cells (MSCs) have been shown to enhance hematopoietic stem/progenitor cell (HSPC) expansion in vitro, expanded cells do not engraft long term in human recipients. This outcome is attributed to the failure of 2D culture to recapitulate the bone marrow (BM) niche signal milieu. Herein, we evaluated the capacity of a novel three-dimensional (3D) coculture system to support HSPC expansion in vitro. A high-throughput polydimethylsiloxane (PDMS) microwell platform was used to manufacture thousands of uniform 3D multicellular coculture spheroids...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28346794/comparative-study-of-xenobiotic-free-media-for-the-cultivation-of-human-limbal-epithelial-stem-progenitor-cells
#18
Sheyla González, Luxia Chen, Sophie X Deng
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications. We quantitatively compared different xenobiotic-free culture media for the cultivation of human LSCs. LSCs were cultured from 2 × 2 mm limbal tissue explants on denuded human amniotic membrane with different xenobiotic-free culture media: CnT-Prime (CnT-PR) supplemented with 0%, 1%, 5%, and 10% human serum (HS), embryonic stem cell medium (ESCM) alone or in combination with the standard supplemented hormonal epithelium medium (SHEM, control) at a 1:1 dilution ratio, and modified SHEM (mSHEM), in which cholera toxin and dimethyl sulfoxide (DMSO) were removed, isoproterenol was added, and the epidermal growth factor concentration was reduced...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28338443/fluorescent-labeling-of-collagen-production-by-cells-for-noninvasive-imaging-of-extracellular-matrix-deposition
#19
Katie Bardsley, Ying Yang, Alicia J El Haj
Extracellular matrix (ECM) is an essential component of tissues and provides both integrity and biological cues for cells. Collagen is one of the major proteins found within the ECM and therefore is an essential component of all engineered tissues. Therefore, in this article, we present a method for the online real-time monitoring of collagen deposition in three-dimensional engineered constructs. This method revolves around modification of collagen through the addition of azide-L-proline to cell culture media...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28338428/a-surgical-device-to-study-the-efficacy-of-bioengineered-skin-substitutes-in-mice-wound-healing-models
#20
Marc G Jeschke, Ali-Reza Sadri, Cassandra Belo, Saeid Amini-Nik
Due to the poor regenerative capacity of adult mammalian skin, there is a need to develop effective skin substitutes for promoting skin regeneration after a severe wound. However, the complexity of skin biology has made it difficult to enable perfect regeneration of skin. Thus, animal models are being used to test potential skin substitutes. Murine models are valuable but their healing process involves dermal contraction. We have developed a device called a dome that is able to eliminate the contraction effect of rodent skin while simultaneously housing a bioengineered skin graft...
April 2017: Tissue Engineering. Part C, Methods
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