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Tissue Engineering. Part C, Methods

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https://www.readbyqxmd.com/read/29336215/non-invasive-real-time-assessment-of-cell-viability-in-a-three-dimensional-tissue
#1
Seyed Hossein Mahfouzi, Ghassem Amoabediny, Ali Doryab, Seyed Hamid Safiabadi-Tali, Mostafa Ghanei
Maintaining cell viability within 3D tissue engineering scaffolds is an essential step toward a functional tissue or organ. Assessment of cell viability in 3D scaffolds is necessary to control and optimize tissue culture process. Monitoring systems based on respiration activity of cells (e.g., oxygen consumption) have been used in various cell cultures. In this research, an online monitoring system based on respiration activity was developed to monitor cell viability within acellular lung scaffolds. First, acellular lung scaffolds were recellularized with human umbilical cord vein endothelial cells, and then, cell viability was monitored during a 5-day period...
January 16, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29327669/advanced-3d-models-culture-to-investigate-mesenchymal-stromal-cells-of-the-human-dental-follicle
#2
Nathalie Steimberg, Francesca Angiero, Davide Farronato, Angiola Berenzi, Gianguido Cossellu, Andrea Ottonello, Darnell Kaigler, Giovanna Mazzoleni
The human dental follicle (hDF) contains the developing tooth and is involved in regulating tooth maturation and eruption. To investigate the mesenchymal stromal cells of the dental follicle, two 3D culture models were used, based on a dynamic bioreactor: the Rotary Cell Culture System (RCCS) and the 3D culture of precursor cells isolated from follicular tissue (hDFCs). The hDFCs were obtained from impacted third molars of 20 patients. Two 3D culture models were tested. In the first model, intact hDF explants were cultured in 3D conditions, preserving the original tissue architecture; they were studied via histomorphological and molecular analyses...
January 12, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29298406/a-miniature-swine-model-for-stem-cell-based-de-novo-regeneration-of-dental-pulp-and-dentin-like-tissue
#3
Xiaofei Zhu, Jie Liu, Zongdong Yu, Chao-An Chen, Hacer Aksel, Adham A Azim, George T-J Huang
The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls...
January 3, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29258387/microfluidic-encapsulation-supports-stem-cell-viability-proliferation-and-neuronal-differentiation
#4
Lorena Hidalgo San Jose, Phil Stephens, Bing Song, David Barrow
Stem cell encapsulation technology demonstrates much promise for the replacement of damaged tissue in several diseases, including spinal cord injury (SCI). The use of biocompatible microcapsules permits the control of stem cell fate in situ to facilitate the replacement of damaged/lost tissue. In this work, a novel customized microfluidic device was developed for the reproducible encapsulation of Neural Stem Cells (NSCs) and Dental Pulp Stem Cells (DPSCs) within monodisperse, alginate-collagen microcapsules...
December 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29241418/isolation-and-characterization-of-mesenchymal-stromal-cells-based-on-aldehyde-dehydrogenase-activity
#5
Mehdi Najar, Laurent Dollé, Emerence Crompot, Stefaan Verhulst, Leo Van Grunsven, Hélène Busser, Laurence Lagneaux
Mesenchymal stromal cells (MSCs) have particular properties that allow their use as therapeutic strategies for several cell-based applications. Historically, bone marrow (BM)-MSCs are isolated by culture adherence since specific cell surface markers are yet to be developed. This original work aimed to identify and characterize isolating expanded BM-MSCs based on their aldehyde dehydrogenase (ALDH) activity known to be a hallmark of stem cells and relevant for their isolation. We thus isolated by FACS technology two functionally different populations of BM-MSCs depending on their ALDH activity (ALDH+ and ALDH-)...
December 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29239281/methods-for-expansion-of-3d-cultures-of-human-embryonic-stem-cells-using-a-thermoresponsive-polymer
#6
Xiaoli Chen, Linda Harkness, Zhongfan Jia, Andrew Prowse, Michael Monteiro, Peter P Gray
Human pluripotent stem cells (hPSCs) are viewed as promising candidates for applications in regenerative medicine and therapy due to their proliferative and pluripotent properties. However, obtaining clinically significant numbers of hPSCs remains a limiting factor and impedes their use in therapeutic applications. Conventionally, hPSCs are cultured on 2-dimensional (2D) surfaces coated with a suitable substrate, such as Matrigel™. This method, however, requires a large surface area to generate sufficient cell numbers to meet clinical needs, and is therefore impractical as a manufacturing platform for cell expansion...
December 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28967302/development-of-in-vitro-drug-induced-cardiotoxicity-assay-by-using-three-dimensional-cardiac-tissues-derived-from-human-induced-pluripotent-stem-cells
#7
Maki Takeda, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Emiko Ito, Akima Harada, Ryohei Matsuura, Hiroko Iseoka, Nagako Sougawa, Noriko Mochizuki-Oda, Michiya Matsusaki, Mitsuru Akashi, Yoshiki Sawa
An in vitro drug-induced cardiotoxicity assay is a critical step in drug discovery for clinical use. The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is promising for this purpose. However, single hiPSC-CMs are limited in their ability to mimic native cardiac tissue structurally and functionally, and the generation of artificial cardiac tissue using hiPSC-CMs is an ongoing challenging. We therefore developed a new method of constructing three-dimensional (3D) artificial tissues in a short time by coating extracellular matrix (ECM) components on cell surfaces...
November 17, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29141507/sandwiched-white-adipose-tissue-a-novel-microphysiological-system-of-human-adipose-tissue
#8
Frank H Lau, Kelly Vogel, John P Luckett, Maxwell Hunt, Alicia Meyer, Camille L Rogers, Oren Tessler, Charles L Dupin, Hugo St Hilaire, Kazi N Islam, Trivia Frazier, Jeffrey Gimble, Steven Scahill
White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. Here we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered sheets of adipose-derived stromal cells. This construct, called "sandwiched white adipose tissue" (SWAT), can be defined as a Microphysiological System (MPS) since it is a tissue-engineered, multicellular, 3D organ construct produced using human cells...
November 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29108480/short-term-homing-of-hyaluronan-primed-cells-therapeutic-implications-for-osteoarthritis-treatment
#9
Giovanna Desando, Isabella Bartolotti, Carola Cavallo, Antonella Schiavinato, Cynthia Secchieri, Elizaveta Kon, Giuseppe Filardo, Maurizio Paro, Brunella Grigolo
The evaluation of key factors modulating cell homing following transplantation can provide new insights in the comprehension of unsolved biological questions about the use of cell therapies for osteoarthritis (OA). The main purpose of this in vivo study was to investigate the biodistribution of an intra-articular (IA) injection of mesenchymal stromal cells (MSCs) and bone marrow concentrate (BMC) in a rabbit OA model, and whether the additional use of sodium hyaluronate (HA) could modulate their migration and promote joint repair...
November 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28922978/six-year-old-sheep-as-a-clinically-relevant-large-animal-model-for-aortic-valve-replacement-using-tissue-engineered-grafts-based-on-decellularized-allogenic-matrix
#10
Karolina Theodoridis, Igor Tudorache, Serghei Cebotari, Alexandru Calistru, Tanja Meyer, Samir Sarikouch, Christoph Bara, Axel Haverich, Andres Hilfiker
Tissue-engineered (TE) grafts based on decellularized grafts have shown very promising results in preclinical and clinical studies. However, in animal models valves have either been tested in juvenile models or in the clinically less relevant pulmonary valve position. In this study, we tested the grafts in the aortic valve (AV) position of 6-year-old sheep, as geriatric patients in need of an AV substitute due to calcification are the largest patient group benefiting from TE grafts. Decellularized AV (DAV; n = 4) and DAV additionally re-endothelialized with autologous cells (n = 3) were implanted in the AV position of 6-year-old female sheep...
November 3, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29092692/characterization-of-multi-layered-tissue-engineered-human-alveolar-bone-and-gingival-mucosa
#11
Thafar Almela, Sarmad Al-Sahaf, Robert Bolt, Ian Brook, K Moharamzadeh
Advances in tissue engineering have permitted assembly of multi-layered composite tissue constructs for potential applications in the treatment of combined hard and soft tissue defects and as an alternative in vitro test model to animal experimental systems. The aim of this study was to develop and characterize a novel three-dimensional combined human alveolar bone and gingival mucosal model based on primary cells isolated from the oral tissues. Bone component of the model was engineered by seeding primary human alveolar osteoblasts (HAOBs) into a hydroxyapatite/tricalcium phosphate (HA/TCP) scaffold and culturing in a spinner bioreactor...
November 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29092689/animal-models-in-tissue-engineering-part-ii
#12
Jorge Piedrahita, J Koudy Williams
Introduction letter to the Animal Models in Tissue Engineering Part II.
November 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29092672/the-impact-of-age-on-skeletal-muscle-progenitor-cell-survival-and-fate-after-injury
#13
Yu Zhou, Daniel Lovell, Maigen Bethea, Benyam Yoseph, James Poteracki, Shay Soker, Tracy Criswell
Sarcopenia is defined as the loss of skeletal muscle mass and function due to age, and represents a major cause of disability in the elderly population. The contributing factors to the onset of sarcopenia are not well defined, but appear to involve age-dependent changes in both the tissue micro-environment and muscle progenitor cell (MPC) population. MPC transplantation has the potential to be a novel therapy for treatment of muscle dysfunction due to aging or injury, but has not shown significant clinical efficacy to date...
November 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756748/the-new-zealand-white-rabbit-as-a-model-for-preclinical-studies-addressing-tissue-repair-at-the-level-of-the-abdominal-wall
#14
Juan M Bellón, Marta Rodríguez, Bárbara Pérez-Köhler, Paloma Pérez-López, Gemma Pascual
In this report, we review the use of the New Zealand White rabbit as the experimental animal for several models of abdominal wall repair. For the repair of an abdominal wall defect, such as a hernia in clinical practice, multiple types of prosthetic material exist. Before their marketing, each of these biomaterials needs to be tested in a preclinical setting to confirm its biocompatibility and appropriate behavior at the different tissue interfaces. For preclinical trials, we have always used the New Zealand White rabbit as the model owing to its ease of handling and suitable size...
October 27, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28795622/animal-models-for-periodontal-tissue-engineering-a-knowledge-generating-process
#15
Karim M Fawzy El-Sayed, Christof E Dörfer
The human periodontium is a uniquely complex vital structure, supporting and anchoring the teeth in their alveolar sockets, thereby playing a decisive role in tooth homeostasis and function. Chronic periodontitis is a highly prevalent immune-inflammatory disease of the periodontium, affecting 15% of adult individuals, and is characterized by progressive destruction of the periodontal tooth-investing tissues, culminating in their irreversible damage. Current periodontal evidence-based treatment strategies achieve periodontal healing via repair processes, mostly combating the inflammatory component of the disease, to halt or reduce prospective periodontal tissue loss...
October 25, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28938849/animal-models-in-tissue-engineering-part-i
#16
Jorge A Piedrahita, J Koudy Williams
Animal models play a central and pivotal role in tissue engineering. Although advances in areas such as 3D printing and bioreactor technologies now permit the in vitro development and testing of complex scaffold/cell composites, in vivo testing remains critical not only for refining methods being developed but also for the critical efficacy and safety testing required for regulatory approval. Yet, choosing the appropriate model for a particular application remains a challenge, as each model has its own strengths and weaknesses...
November 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#17
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
November 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28795641/fixation-of-hydrogel-constructs-for-cartilage-repair-in-the-equine-model-a-challenging-issue
#18
Irina A D Mancini, Rafael A Vindas Bolaños, Harold Brommer, Miguel Castilho, Alexandro Ribeiro, Johannes P A M van Loon, Anneloes Mensinga, Mattie H P van Rijen, Jos Malda, René van Weeren
OBJECTIVE: To report on the experiences with the use of commercial and autologous fibrin glue (AFG) and of an alternative method based on a 3D-printed polycaprolactone (PCL) anchor for the fixation of hydrogel-based scaffolds in an equine model for cartilage repair. METHODS: In a first study, three different hydrogel-based materials were orthotopically implanted in nine horses for 1-4 weeks in 6 mm diameter full-thickness cartilage defects in the medial femoral trochlear ridge and fixated with commercially available fibrin glue (CFG)...
November 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762895/encapsulation-of-equine-endothelial-colony-forming-cells-in-highly-uniform-injectable-hydrogel-microspheres-for-local-cell-delivery
#19
Wen J Seeto, Yuan Tian, Randolph L Winter, Fred J Caldwell, Anne A Wooldridge, Elizabeth A Lipke
A common challenge in cell therapy is the inability to routinely maintain survival and localization of injected therapeutic cells. Delivering cells by direct injection increases the flexibility of clinical applications, but may cause low cell viability and retention rates due to the high shear forces in the needle and mechanical wash out. In this study, we encapsulated endothelial colony forming cells (ECFCs) in poly(ethylene glycol)-fibrinogen (PF) hydrogel microspheres using a custom-built microfluidic device; this system supports rapid encapsulation of high cell concentrations (10 million cells per mL) and resulting cell-laden microspheres are highly uniform in shape and size...
November 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28762881/porcine-ischemic-wound-healing-model-for-preclinical-testing-of-degradable-biomaterials
#20
Prarthana Patil, John R Martin, Samantha M Sarett, Alonda C Pollins, Nancy L Cardwell, Jeffrey M Davidson, Scott A Guelcher, Lillian B Nanney, Craig L Duvall
Impaired wound healing that mimics chronic human skin pathologies is difficult to achieve in current animal models, hindering testing and development of new therapeutic biomaterials that promote wound healing. In this article, we describe a refinement and simplification of the porcine ischemic wound model that increases the size and number of experimental sites per animal. By comparing three flap geometries, we adopted a superior configuration (15 × 10 cm) that enabled testing of twenty 1 cm(2) wounds in each animal: 8 total ischemic wounds within 4 bipedicle flaps and 12 nonischemic wounds...
November 2017: Tissue Engineering. Part C, Methods
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