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Molecular Plant

Yunming Zhang, Michael Lenhard
No abstract text is available yet for this article.
May 17, 2017: Molecular Plant
Lucie Camut, Jean-Michel Davière, Patrick Achard
No abstract text is available yet for this article.
May 12, 2017: Molecular Plant
Xiaolu Qu, Ruihui Zhang, Meng Zhang, Min Diao, Yongbiao Xue, Shanjin Huang
Polarized tip growth is a fundamental cellular process in many eukaryotes. In this study, we examined the dynamic restructuring of the actin cytoskeleton and its relationship to vesicle transport during pollen tip growth in Arabidopsis. We found that actin filaments originating from the apical membrane form a specialized structure consisting of longitudinally aligned actin bundles at the cortex and inner cytoplasmic filaments with a distinct distribution. Using actin-based pharmacological treatments and genetic mutants in combination with FRAP (fluorescence recovery after photobleaching) technology to visualize the transport of vesicles within the growth domain of pollen tubes, we demonstrate that cortical actin filaments facilitate tip-ward vesicle transport...
May 10, 2017: Molecular Plant
Jian Chen, En Li, Xiangbo Zhang, Xiaomei Dong, Lei Lei, Weibin Song, Haiming Zhao, Jinsheng Lai
Nucleosomes are the fundamental units of chromatin and play critical roles in gene regulation by modulating DNA accessibility, but their roles in transcriptional tissue specificity are poorly understood. Here, we present genome-wide nucleosome maps of maize shoot and endosperm generated by sequencing the micrococcal nuclease digested nucleosomal DNA. The changes of gene transcriptional status between shoot and endosperm were accompanied by preferential nucleosome loss from the promoters and shifts in the first nucleosome downstream of the transcriptional start sites (+1 nucleosome) and upstream of transcriptional termination sites (-1 nucleosome)...
May 6, 2017: Molecular Plant
Baobao Wang, Haiyang Wang
No abstract text is available yet for this article.
May 3, 2017: Molecular Plant
Duoduo Wang, Graham B Seymour
No abstract text is available yet for this article.
May 3, 2017: Molecular Plant
En-Hua Xia, Hai-Bin Zhang, Jun Sheng, Kui Li, Qun-Jie Zhang, Changhoon Kim, Yun Zhang, Yuan Liu, Ting Zhu, Wei Li, Hui Huang, Yan Tong, Hong Nan, Cong Shi, Chao Shi, Jian-Jun Jiang, Shu-Yan Mao, Jun-Ying Jiao, Dan Zhang, Yuan Zhao, You-Jie Zhao, Li-Ping Zhang, Yun-Long Liu, Ben-Ying Liu, Yue Yu, Sheng-Fu Shao, De-Jiang Ni, Evan E Eichler, Li-Zhi Gao
Tea is the world's oldest and most popular caffeine-containing beverage with immense economic, medicinal, and cultural importance. Here, we present the first high-quality nucleotide sequence of the repeat-rich (80.9%), 3.02-Gb genome of the cultivated tea tree Camellia sinensis. We show that an extraordinarily large genome size of tea tree is resulted from the slow, steady, and long-term amplification of a few LTR retrotransposon families. In addition to a recent whole-genome duplication event, lineage-specific expansions of genes associated with flavonoid metabolic biosynthesis were discovered, which enhance catechin production, terpene enzyme activation, and stress tolerance, important features for tea flavor and adaptation...
April 29, 2017: Molecular Plant
Victor Aguilar-Hernández, Do-Young Kim, Robert J Stankey, Mark Scalf, Lloyd M Smith, Richard D Vierstra
The skotomorphogenic to photomorphogenic switch is a key developmental transition in the life of seed plants. While much of the underpinning proteome remodeling is driven by light-induced changes in gene expression, the proteolytic removal of specific proteins by the ubiquitin-26S proteasome system is also likely paramount. Through mass spectrometric analysis of ubiquitylated proteins affinity purified from etiolated Arabidopsis seedlings before and after red-light irradiation, we identified a number of influential proteins whose ubiquitylation status is modified during this switch...
April 28, 2017: Molecular Plant
David Heckmann, Urte Schlüter, Andreas P M Weber
Harnessing natural variation in photosynthetic capacity is a promising route towards yield increases, but physiological phenotyping is still too laborious for large scale genetic screens. Here, we evaluate the potential of leaf reflectance spectroscopy to predict parameters of photosynthetic capacity in Brassica oleracea and Zea mays, a C3 and a C4 crop, respectively. To this end we systematically evaluate properties of reflectance spectra and find that they are surprisingly similar over a wide range of species...
April 28, 2017: Molecular Plant
Tak Lee, Sohyun Hwang, Chan Yeong Kim, Hongseok Shim, Hyojin Kim, Pamela C Ronald, Edward M Marcotte, Insuk Lee
No abstract text is available yet for this article.
April 24, 2017: Molecular Plant
Lin-Hui Yu, Jie Wu, Zi-Sheng Zhang, Zi-Qing Miao, Ping-Xia Zhao, Zhen Wang, Cheng-Bin Xiang
Seed germination is a crucial checkpoint for plant survival under unfavorable environmental conditions. Abscisic acid (ABA) and its signaling play a vital role in integrating environmental information to regulate seed germination. MCM1/AGAMOUS/DEFICIENS/SRF (MADS)-box transcription factors are mainly known as key regulators of seed and flower development in Arabidopsis. However, little is known about their functions in seed germination. Here we report that MADS-box transcription factor AGL21 negatively modulates seed germination and post-germination growth by controlling the expression of ABA-INSENSITIVE 5 (ABI5) in Arabidopsis...
April 21, 2017: Molecular Plant
William Underwood, Andrew Ryan, Shauna C Somerville
Deposition of cell wall-reinforcing papillae is an integral component of the plant immune response. The Arabidopsis PENETRATION 3 (PEN3) ATP binding cassette (ABC) transporter plays a role in defense against numerous pathogens and is recruited to sites of pathogen detection where it accumulates within papillae. However, the trafficking pathways and regulatory mechanisms contributing to recruitment of PEN3 and other defenses to the host-pathogen interface are poorly understood. Here, we report a confocal microscopy-based screen to identify mutants with altered localization of PEN3-GFP after inoculation with powdery mildew fungi...
April 18, 2017: Molecular Plant
Carlos Perea-Resa, Miguel A Rodríguez-Milla, Elisa Iniesto, Vicente Rubio, Julio Salinas
The process of cold acclimation is an important adaptive response whereby many plants from temperate regions increase their freezing tolerance after being exposed to low-nonfreezing temperatures. The correct development of this response relies on the precise accumulation of a number of transcription factors that regulate the appropriate patterns of cold-responsive gene expression. Multiple studies have revealed a variety of molecular mechanisms involved in promoting the accumulation of these transcription factors...
April 12, 2017: Molecular Plant
Sarfraz Shafiq, Chunli Chen, Jing Yang, Lingling Cheng, Fei Ma, Emilie Widemann, Qianwen Sun
R-loop structures (RNA:DNA hybrids) have important functions in many biological processes, including transcriptional regulation and genome instability among diverse organisms. DNA topoisomerase 1 (TOP1), an essential manipulator of DNA topology during RNA transcription and DNA replication processes, can prevent R-loop accumulation by removing the positive and negative DNA supercoiling that is made by RNA polymerases during transcription. TOP1 is required for plant development, but little is known about its function in preventing co-transcriptional R-loop accumulation in various biological processes in plants...
April 12, 2017: Molecular Plant
Feng Liu, Huiyang Yu, Yingtian Deng, Jingyuan Zheng, Minglei Liu, Lijun Ou, Bozhi Yang, Xiongze Dai, Yanqing Ma, Shengyu Feng, Shuang He, Xuefeng Li, Zhuqing Zhang, Wenchao Chen, Shudong Zhou, Rong Chen, Minmin Liu, Sha Yang, Ruimin Wei, Huadong Li, Feng Li, Bo Ouyang, Xuexiao Zou
No abstract text is available yet for this article.
March 24, 2017: Molecular Plant
Mugui Wang, Yanfei Mao, Yuming Lu, Xiaoping Tao, Jian-Kang Zhu
No abstract text is available yet for this article.
March 16, 2017: Molecular Plant
Mugui Wang, Yuming Lu, José Ramón Botella, Yanfei Mao, Kai Hua, Jian-Kang Zhu
No abstract text is available yet for this article.
March 16, 2017: Molecular Plant
Qinjie Chu, Xingchen Zhang, Xintian Zhu, Chen Liu, Lingfeng Mao, Chuyu Ye, Qian-Hao Zhu, Longjiang Fan
No abstract text is available yet for this article.
March 15, 2017: Molecular Plant
Wei Chen, Ling Kui, Guanghui Zhang, Shusheng Zhu, Jing Zhang, Xiao Wang, Min Yang, Huichuan Huang, Yixiang Liu, Yong Wang, Yahe Li, Lipin Zeng, Wen Wang, Xiahong He, Yang Dong, Shengchao Yang
No abstract text is available yet for this article.
March 15, 2017: Molecular Plant
Dan Zhang, Wei Li, En-Hua Xia, Qun-Jie Zhang, Yuan Liu, Yun Zhang, Yan Tong, Yuan Zhao, Yong-Chao Niu, Jia-Huan Xu, Li-Zhi Gao
No abstract text is available yet for this article.
March 14, 2017: Molecular Plant
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