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New Biotechnology

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https://www.readbyqxmd.com/read/28803126/fed-batch-production-of-vanillin-by-bacillus-aryabhattai-ba03
#1
Alicia Paz, David Outeiriño, Ricardo Pinheiro de Souza Oliveira, José Manuel Domínguez
Bacillus aryabhattai BA03, a strain isolated in our laboratory, has interesting properties related to the production of natural aromas and flavors. Specifically, we have found that it was able to produce vanillin from ferulic acid (FA). Furthermore, this strain produces high amounts of 4-vinylguaiacol in only 14h, this being the only intermediate metabolite observed in the process. FA is an inexpensive feedstock for the production of natural value-added compounds when extracted from lignocellulosic wastes. In this study, we optimized the operational conditions (temperature, pH and agitation), medium composition and bioconversion technology (batch or fed-batch) to produce vanillin...
August 9, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28801256/new-innovative-ecosystems-in-france-to-develop-the-bioeconomy
#2
REVIEW
Thierry Stadler, Jean-Marie Chauvet
The cluster's policy initiated in France in 2005 was a formidable opportunity to boost innovation in the bioeconomy and to develop innovation platforms allowing industrial demonstration and feasibility at technology readiness level (TRL) from 5 to 9. The IAR Cluster (Industries and Agro-Resources), based in the Hauts-de-France and Grand Est regions is completely dedicated to the bioeconomy. IAR's work has contributed to build a complete innovation ecosystem on bioeconomy by opening up relationships between actors along the full value chain...
August 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28778817/production-of-virus-like-particles-for-vaccines
#3
J Fuenmayor, F Gòdia, L Cervera
Virus-like particles (VLPs) are nanostructures that resemble the structures of viruses. They are composed of one or more structural proteins that can be arranged in several layers and can also contain a lipid outer envelope. VLPs trigger a high humoral and cellular immune response due to their repetitive structures. A key factor regarding VLP safety is the lack of viral genomic material, which enhances safety during both manufacture and administration. Contemporary VLP production may take advantage of several systems, including bacterial, yeast, insect and mammalian cells...
August 1, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28778816/combination-of-phage-and-gram-positive-bacterial-display-of-human-antibody-repertoires-enables-isolation-of-functional-high-affinity-binders
#4
Francis Jingxin Hu, Anna-Luisa Volk, Helena Persson, Anna Säll, Carl Borrebaeck, Mathias Uhlen, Johan Rockberg
Surface display couples genotype with a surface exposed phenotype and thereby allows screening of gene-encoded protein libraries for desired characteristics. Of the various display systems available, phage display is by far the most popular, mainly thanks to its ability to harbour large size libraries. Here, we describe the first use of a Gram-positive bacterial host for display of a library of human antibody genes which, when combined with phage display, provides ease of use for screening, sorting and ranking by flow cytometry...
August 1, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28743564/evolving-biocatalysis-to-meet-bioeconomy-challenges-and-opportunities
#5
REVIEW
Alessandro Pellis, Sara Cantone, Cynthia Ebert, Lucia Gardossi
The unique selectivity of enzymes, along with their remarkable catalytic activity, constitute powerful tools for transforming renewable feedstock and also for adding value to an array of building blocks and monomers produced by the emerging bio-based chemistry sector. Although some relevant biotransformations run at the ton scale demonstrate the success of biocatalysis in industry, there is still a huge untapped potential of catalytic activities available for targeted valorization of new raw materials, such as waste streams and CO2...
July 22, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28736192/the-presence-of-phb-granules-in-cytoplasm-protects-non-halophilic-bacterial-cells-against-the-harmful-impact-of-hypertonic-environments
#6
Stanislav Obruca, Petr Sedlacek, Filip Mravec, Vladislav Krzyzanek, Jana Nebesarova, Ota Samek, Dan Kucera, Pavla Benesova, Kamila Hrubanova, Miluse Milerova, Ivana Marova
Numerous prokaryotes accumulate polyhydroxybutyrate (PHB) intracellularly as a storage material. It has also been proposed that PHB accumulation improves bacterial stress resistance. Cupriavidus necator and its PHB non-accumulating mutant were employed to investigate the protective role of PHB under hypertonic conditions. The presence of PHB granules enhanced survival of the bacteria after exposure to hypertonic conditions. Surprisingly, when coping with such conditions, the bacteria did not utilize PHB to harvest carbon or energy, suggesting that, in the osmotic upshock of C...
July 20, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28720419/isolation-of-two-ochrobactrum-sp-strains-capable-of-degrading-the-nootropic-drug-piracetam
#7
Marta Woźniak-Karczewska, Monika Čvančarová, Łukasz Chrzanowski, Boris Kolvenbach, Philippe F-X Corvini, Danuta Cichocka
Piracetam (2-oxo-1-pyrrolidine acetamide) is a popular cognitive enhancer, which has recently been detected in waste and drinking water. Nootropic drugs are designed to affect human metabolism and act on the nervous system, but their environmental effects have yet to be the subject of detailed studies. In this report, we present the efficient biodegradation of the cognitive enhancer, piracetam. Two bacterial strains capable of using this compound as the sole carbon source were isolated and later identified as Ochrobactrum anthropi strain MW6 and Ochrobactrum intermedium strain MW7...
July 15, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28716653/bioeconomy-in-latin-america
#8
REVIEW
Albert Sasson, Carlos Malpica
This article provides the authors' view on how Latin America has embraced bioeconomy principles in the last two decades with different levels of socio-economic impact. Examples of biodiversity resource valorization in medicine, eco-intensification of agriculture, biotechnology applications in mature sectors such as mining, food and beverage production, bio-refineries and ecosystem services are provided. The importance of participatory and social innovation initiatives is highlighted.
July 14, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28711520/exploring-substrate-specificities-of-a-recombinant-rhizopus-oryzae-lipase-in-biodiesel-synthesis
#9
Albert Canet, M Dolors Benaiges, Francisco Valero, Patrick Adlercreutz
The alcoholysis of triolein was used to explore the specific features of a recombinant Rhizopus oryzae lipase (rROL) for biodiesel synthesis. For this purpose, different acylglycerols were compared as substrates in lipase-catalysed transesterification. rROL was shown to exhibit a higher specificity towards 1-monoolein than triolein compared to other R. oryzae lipases, being more than 4-fold more specific; in contrast, rROL did not accept 2-monoolein as substrate, concluding that it is highly 1,3-positional specific...
July 12, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28711519/perspectives-on-bioeconomy
#10
EDITORIAL
Alfredo Aguilar, Roland Wohlgemuth, Tomasz Twardowski
The insight provided by the authors of this special issue on bioeconomy give a reassuring sign of optimism, vitality and strong will to make bioeconomy a success story. Key to these advances will be inventing and shaping our future and the cooperation of people who will create new scientific and technological discoveries, developments and their implementation into industrial practice. The vibrant global megatrend bioeconomy is developing along various dimensions depending on natural and social conditions, economic development and political objectives...
July 12, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28698131/white-grape-pomace-extracts-obtained-by-a-sequential-enzymatic-plus-ethanol-based-extraction-exert-antioxidant-anti-tyrosinase-and-anti-inflammatory-activities
#11
Maura Ferri, Greta Rondini, Maria Maddalena Calabretta, Elisa Michelini, Veronica Vallini, Fabio Fava, Aldo Roda, Giordano Minnucci, Annalisa Tassoni
The present work aimed at optimizing a two-step enzymatic plus solvent-based process for the recovery of bioactive compounds from white grape (Vitis vinifera L., mix of Trebbiano and Verdicchio cultivars) pomace, the winemaking primary by-product. Phenolic compounds solubilised by water enzyme-assisted and ethanol-based extractions of wet (WP) and dried (DP) pomace were characterized for composition and tested for antioxidant, anti-tyrosinase and anti-inflammatory bioactivities. Ethanol treatment led to higher phenol yields than water extraction, while DP samples showed the highest capacity of releasing polyphenols, most probably as a positive consequence of the pomace drying process...
July 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28698130/enzymatic-hydrolysis-of-poly-ethyleneterephthalate-used-for-and-analysed-by-pore-modification-of-track-etched-membranes
#12
Caroline Gamerith, Martyna Gajda, Andreas Ortner, Enrique Herrero Acero, Georg M Guebitz, Mathias Ulbricht
The potential of limited enzymatic poly(ethylene terephthalate) (PET) surface hydrolysis for the modification of track-etched (TE) membranes was investigated. Cutinases 1 and 2 from Thermobifida cellulosilytica as well as a fusion protein of cutinase 1 with the polymer binding module from the polyhydroxyalkanoate depolymerase of Alcaligenes faecalis (Thc_Cut1_PBM) were shown to hydrolyse highly crystalline PET TE membranes with a pore diameter of ∼120nm at very narrow size distribution. Furthermore the effects of surface chemistry were investigated by comparison of enzymatic hydrolysis by Thc_Cut1_PBM of "as received" PET TE membranes with two surface functionalized versions towards a "hydrophilic" and a more "hydrophobic" surface...
July 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28698129/an-efficient-agro-industrial-complex-in-almer%C3%A3-a-spain-towards-an-integrated-and-sustainable-bioeconomy-model
#13
REVIEW
Francisco J Egea, Roberto G Torrente, Alfredo Aguilar
In the last ten years, bioeconomy strategies and policy-related bioeconomy initiatives have been developed all over the world. Some of them are currently in the process of translation into specific actions. In most cases, the approaches followed have been top-down policy-related initiatives, triggered by the public sector originating a dynamic which can bring together different bioeconomy stakeholders i.e. industry, academia, financial operators and farmers. This article describes a bottom-up situation with unique bioeconomy-related features that deserve specific attention...
July 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28690157/cell-free-protein-synthesis-in-micro-compartments-building-a-minimal-cell-from-biobricks
#14
REVIEW
Haiyang Jia, Michael Heymann, Frank Bernhard, Petra Schwille, Lei Kai
The construction of a minimal cell that exhibits the essential characteristics of life is a great challenge in the field of synthetic biology. Assembling a minimal cell requires multidisciplinary expertise from physics, chemistry and biology. Scientists from different backgrounds tend to define the essence of 'life' differently and have thus proposed different artificial cell models possessing one or several essential features of living cells. Using the tools and methods of molecular biology, the bottom-up engineering of a minimal cell appears in reach...
July 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28690156/the-microbiome-as-engineering-tool-manufacturing-and-trading-between-microorganisms
#15
REVIEW
Jo De Vrieze, Marlies E R Christiaens, Willy Verstraete
The integration of microbial technologies within the framework of the water-energy nexus has been taking place for over a century, but these mixed microbial communities are considered hard to deal with 'black boxes'. Process steering is mainly based on avoiding process failure by monitoring conventional parameters, e.g., pH and temperature, which often leads to operation far below the intrinsic potential. Mixed microbial communities do not reflect a randomised individual mix, but an interacting microbiological entity...
July 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28690155/plant-breeding-as-the-cornerstone-of-a-sustainable-bioeconomy
#16
REVIEW
Aleksandra Małyska, Jan Jacobi
A prime driver for a bioeconomy is the need to ensure the availability of sufficient biomass feedstock for food, feed, energy and industrial uses. This demand must be properly managed in the face of several challenges, including environmental changes and abrupt climate shifts. Plant breeding and breeding innovation is the cornerstone for sustainable supply of biomass. Not only does research and development in this sector aim at providing high yielding crops in order to maximize production, but R&D in this field will also allow to obtain highly specialized plant varieties with new or improved traits that fit to specific applications...
July 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28676418/waste-derived-bioeconomy-in-india-a-perspective
#17
REVIEW
Venkata Mohan S, Chiranjeevi P, Shikha Dahiya, Naresh Kumar A
Environmental and climatic change issues, population explosion, rapid urbanization, depletion of fossil reserves, need for energy security, huge waste generation, etc. are some of the inherent issues associated with the fossil based linear economy which need sustainable attention. In this realm, the world is gradually transforming from fossil-based economy to a sustainable circular economy. The biogenic waste which is generated in enormous quantity in India can be considered as potential feedstock for structuring the bio-based economy...
July 1, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28676417/eu-ambition-to-build-the-world-s-leading-bioeconomy-uncertain-times-demand-innovative-and-sustainable-solutions
#18
REVIEW
John Bell, Lino Paula, Thomas Dodd, Szilvia Németh, Christina Nanou, Voula Mega, Paula Campos
This article outlines the current context and the development of the European Bioeconomy Strategy. It analyses the current situation, challenges and needs for EU action and concludes with the next steps that the European Commission will undertake to review and update the Bioeconomy Strategy. Bioeconomy offers great opportunities to realising a competitive, circular and sustainable economy with a sound industrial base that is less dependent on fossil carbon. A sustainable bioeconomy also contributes to climate change mitigation, with oceans, forests and soils being major carbon sinks and fostering negative CO2 emissions...
July 1, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28669887/intellectual-property-rights-derived-from-academic-research-and-their-role-in-the-modern-bioeconomy-a-guide-for-scientists
#19
Jan B Krauss, David Kuttenkeuler
No abstract text is available yet for this article.
June 30, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28668584/the-biotechnology-and-bioeconomy-landscape-in-malaysia
#20
REVIEW
Mahaletchumy Arujanan, Muthu Singaram
Since 1990s Malaysia aspired to make biotechnology and bioeconomy as her engines of economic growth to utlise the abundance of natural resources and biodiversity. The public sector plays an integral role in developing the sector and various incentives are in place for the private sector to be actively involved and to forge collaboration with the public sector. The country launched its National Biotechnology Policy in 2005 and later launched its National Bioeconomy Programme in 2010 to become the first country in South East Asia and second in Asia after China to have such an initiative...
June 28, 2017: New Biotechnology
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