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New Biotechnology

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https://www.readbyqxmd.com/read/28069519/denaturing-fixatives-are-compatible-with-the-nanostring-ncounter-%C3%A2-platform-and-the-prosigna-%C3%A2-assay
#1
Roman Rouzier, Aurelie Roulot, Arthur H Jeiranian, Namratha Ram, Jean Marc Guinebretiere, Anne Vincent Salomon, David Gentien
The objective of this study was to establish that the denaturing fixative, formalin and acetic acid (AFA), is equivalent to neutral-buffered formalin (NBF) on the nCounter system using the Prosigna assay. Twenty-five previously resected tumors from breast cancer patients were apportioned and fixed with either NBF or AFA prior to embedding in paraffin. Differentially fixed and paraffin embedded tissue pairs were sectioned and pathological review was performed according to the Prosigna assay protocol. RNA was isolated using the Prosigna assay kit and analyzed using the NanoString nCounter Dx Analysis system...
January 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#2
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
January 5, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28043869/isolation-of-rhamnolipids-producing-cultures-from-faeces-influence-of-interspecies-communication-on-the-yield-of-rhamnolipid-congeners
#3
Marta Woźniak-Karczewska, Kamila Myszka, Agata Sznajdrowska, Alicja Szulc, Agnieszka Zgoła-Grześkowiak, Łukasz Ławniczak, Philippe F-X Corvini, Łukasz Chrzanowski
The aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. Initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce biosurfactants...
December 30, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28043868/evaluation-of-anaerobic-digestates-from-different-feedstocks-as-growth-media-for-tetradesmus-obliquus-botryococcus-braunii-phaeodactylum-tricornutum-and-arthrospira-maxima
#4
Marina Massa, Silvia Buono, Antonio Luca Langellotti, Luigi Castaldo, Anna Martello, Antonello Paduano, Raffaele Sacchi, Vincenzo Fogliano
In this paper, two freshwater microalgae (Tetradesmus obliquus and Botryococcus braunii), a marine diatom (Phaeodactylum tricornutum) and a photosynthetic cyanobacterium (Arthrospira maxima) were investigated for their ability to grow on liquid digestates (LDs). Three LDs were obtained from anaerobic digestion of different organic wastes: zootechnical (ZW LD), vegetable biomass (VW LD) and the organic fraction of municipal solid wastes (MW LD). All the strains showed the same growth performance on VW LD as on the respective standard media (SM), while ZW LD was efficient only for growth of T...
December 30, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28040555/juncus-spp-the-helophyte-for-all-phyto-remediation-purposes
#5
Evdokia Syranidou, Stavros Christofilopoulos, Nicolas Kalogerakis
Helophytic plants contribute significantly to the remediation of ecosystems through a wide range of physiological or biochemical mechanisms including the role of endophytic bacteria. This review highlights the services provided by Juncus spp. wetland plants, from phytoremediation of soils and groundwater with heavy metals and/or organics to municipal or industrial wastewater treatment in constructed wetlands. The data presented also provide information on the efficiency of specific Juncus spp. in response to various metals and organic compounds, in an effort to exploit the natural capabilities of autochthonous over exotic species in phytoremediation strategies...
December 28, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28024971/in-commemoration-of-completion-of-the-genetic-code-and-in-memory-of-prof-brian-clark
#6
Huanming Yang
While we celebrate the 20th anniversary of the complete decoding of the genetic code, we should also commemorate Prof. Brian Clark for his remarkable contributions to this historic landmark in life sciences, as well as to the global collaboration on sciences. As an international citizen and a great scientist, Prof. Brian Clark will live for ever in the hearts of all his friends and students.
December 23, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28011289/immobilization-of-laccase-of-pycnoporus-sanguineus-cs43
#7
Luis A Gonzalez-Coronel, Marta Cobas, Magdalena de J Rostro-Alanis, Roberto Parra-Saldívar, Carlos Hernandez-Luna, Marta Pazos, M Ángeles Sanromán
Laccase from Pycnoporus sanguineus CS43 was successfully immobilized onto Immobead-150 and Eupergit-C by covalent binding and by entrapment in LentiKats. The highest immobilization was onto Immobead-150 (97.1±1.2%) compared to the other supports, LentiKats (89±1.1%) and Eupergit-C (83.2±1.4%). All three immobilized enzyme systems showed increased thermostability and better mechanical properties than free laccase. Moreover, after 5 cycles of reuse of these systems, 90% of initial laccase activity was retained...
December 21, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27988304/overview-of-the-membrane-associated-ring-ch-march-e3-ligase-family
#8
Johannes Bauer, Oddmund Bakke, J Preben Morth
E3 ligases are critical checkpoints for protein ubiquitination, a signal that often results in protein sorting and degradation but has also been linked to regulation of transcription and DNA repair. In line with their key role in cellular trafficking and cell-cycle control, malfunction of E3 ligases is often linked to human disease. Thus, they have emerged as prime drug targets. However, the molecular basis of action of membrane-bound E3 ligases is still unknown. Here, we review the current knowledge on the membrane-embedded MARCH E3 ligases (MARCH-1-6,7,8,11) with a focus on how the transmembrane regions can contribute via GxxxG-motifs to the selection and recognition of other membrane proteins as substrates for ubiquitination...
December 14, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27986600/effect-of-culture-residence-time-on-substrate-uptake-and-storage-by-a-pure-culture-of-thiothrix-ct3-strain-under-continuous-or-batch-feeding
#9
Francesco Valentino, Mario Beccari, Marianna Villano, Valter Tandoi, Mauro Majone
A pure culture of the filamentous bacterium Thiothrix, strain CT3, was aerobically cultured in a chemostat under continuous acetate feeding at three different culture residence times (RT 6, 12 or 22 d) and the same volumetric organic load rate (OLR 0.12gCOD/L/d). Cells cultured at decreasing RT in the chemostat had an increasing transient response to acetate spikes in batch tests. The maximum specific acetate removal rate increased from 25 to 185mgCOD/gCOD/h, corresponding to a 1.8 to 8.1 fold higher respective steady-state rate in the chemostat...
December 13, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27915060/special-issue-of-new-biotechnology-in-memory-of-professor-brian-f-c-clark-1936-2014
#10
EDITORIAL
Francesco Lescai
No abstract text is available yet for this article.
November 30, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27903429/polyhydroxyalkanoate-as-a-slow-release-carbon-source-for-in-situ-bioremediation-of-contaminated-aquifers-from-laboratory-investigation-to-pilot-scale-testing-in-the-field
#11
Lucia Pierro, Bruna Matturro, Simona Rossetti, Marco Sagliaschi, Salvatore Sucato, Eduard Alesi, Ernst Bartsch, Firoozeh Arjmand, Marco Petrangeli Papini
A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs)...
November 27, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27989957/renewable-feedstocks-for-biobutanol-production-by-fermentation
#12
Alessandra Procentese, Francesca Raganati, Giuseppe Olivieri, Maria Elena Russo, Marco De La Feld, Antonio Marzocchella
This paper reports a study of potential feedstock for butanol production via the biotechnological route. Several waste(water) streams rich in sugars and lignocellulosic biomass were studied: cheese-whey, leftovers of high sugar-content beverages, food lost or wasted, agriculture residues. The maximum butanol production rate from each type of feedstock was assessed according to the parameters indicated in the literature: feedstock availability rate, feedstock average composition and butanol yield. In Europe the potential biotechnological production of butanol from the feedstock studied was assessed to be about 39 Mt yr-1, which would be enough to meet the current European demand of biofuels...
October 28, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27793692/strategies-for-efficiently-selecting-pha-producing-mixed-microbial-cultures-using-complex-feedstocks-feast-and-famine-regime-and-uncoupled-carbon-and-nitrogen-availabilities
#13
Catarina S S Oliveira, Carlos E Silva, Gilda Carvalho, Maria A Reis
Production of polyhydroxyalkanoates (PHAs) by open mixed microbial cultures (MMCs) has been attracting increasing interest as an alternative technology to PHA production by pure cultures, due to the potential for lower costs associated with the use of open systems (eliminating the requirement for sterile conditions) and the utilisation of cheap feedstock (industrial and agricultural wastes). Such technology relies on the efficient selection of an MMC enriched in PHA-accumulating organisms. Fermented cheese whey, a protein-rich complex feedstock, has been used previously to produce PHA using the feast and famine regime for selection of PHA accumulating cultures...
October 25, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27989956/pseudoalteromonas-haloplanktis-produces-methylamine-a-volatile-compound-active-against-burkholderia-cepacia-complex-strains
#14
Filomena Sannino, Ermenegilda Parrilli, Gennaro Antonio Apuzzo, Donatella de Pascale, Pietro Tedesco, Isabel Maida, Elena Perrin, Marco Fondi, Renato Fani, Gennaro Marino, Maria Luisa Tutino
The Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 has been reported to produce several Volatile Organic Compounds (VOCs), which are able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. However, no specific antibacterial VOCs have been identified to date. The purpose of the present study was to identify specific VOCs that contribute to Bcc inhibition by the Antarctic strain. When grown on defined medium containing D-gluconate and L-glutamate as carbon, nitrogen and energy sources, P...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27932313/3rd-congress-on-applied-synthetic-biology-in-europe-costa-da-caparica-portugal-february-2016
#15
Miguel Cueva
The third meeting organised by the European Federation of Biotechnology (EFB) on advances in Applied Synthetic Biotechnology in Europe (ASBE) was held in Costa da Caparica, Portugal, in February 2016. Abundant novel applications in synthetic biology were described in the six sessions of the meeting, which was divided into technology and tools for synthetic biology (I, II and III), bionanoscience, biosynthetic pathways and enzyme synthetic biology, and metabolic engineering and chemical manufacturing. The meeting presented numerous methods for the development of novel synthetic strains, synthetic biological tools and synthetic biology applications...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27915059/a-process-for-polyhydroxyalkanoate-pha-production-from-municipal-wastewater-treatment-with-biological-carbon-and-nitrogen-removal-demonstrated-at-pilot-scale
#16
Simon Bengtsson, Anton Karlsson, Tomas Alexandersson, Luca Quadri, Markus Hjort, Peter Johansson, Fernando Morgan-Sagastume, Simon Anterrieu, Monica Arcos-Hernandez, Lamija Karabegovic, Per Magnusson, Alan Werker
A process was developed for biological treatment of municipal wastewater for carbon and nitrogen removal while producing added-value polyhydroxyalkanoates (PHAs). The process comprised steps for pre-denitrification, nitrification and post-denitrification and included integrated fixed-film activated sludge (IFAS) with biofilm carrier media to support nitrification. In a pilot-scale demonstration (500-800L), wastewater treatment performance, in line with European standards, were achieved for total chemical oxygen demand (83% removal) and total nitrogen (80% removal) while producing a biomass that was able to accumulate up to 49% PHA of volatile suspended solids with acetic acid or fermented organic residues as substrates...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27902938/functional-components-of-the-bacterial-czccba-efflux-system-reduce-cadmium-uptake-and-accumulation-in-transgenic-tobacco-plants
#17
Andrea Nesler, Giovanni DalCorso, Elisa Fasani, Anna Manara, Gian Pietro Di Sansebastiano, Emanuele Argese, Antonella Furini
Cadmium (Cd) is a toxic trace element released into the environment by industrial and agricultural practices, threatening the health of plants and contaminating the food/feed chain. Biotechnology can be used to develop plant varieties with a higher capacity for Cd accumulation (for use in phytoremediation programs) or a lower capacity for Cd accumulation (to reduce Cd levels in food and feed). Here we generated transgenic tobacco plants expressing components of the Pseudomonas putida CzcCBA efflux system. Plants were transformed with combinations of the CzcC, CzcB and CzcA genes, and the impact on Cd mobilization was analysed...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27894932/microbial-stress-from-molecules-to-systems-sitges-november-2015
#18
Francesca Martani, Nadia Maria Berterame, Paola Branduardi
The meeting "Microbial Stress: from Molecules to Systems" - the third in this series - was held in Sitges (Spain) in November 2015. The meeting offered the opportunity for international scientists to share their viewpoints and recent outcomes concerning microbial stress responses. Particular attention was given to the characterisation of mechanisms triggered by stress, from detailed molecular biology through whole organism systems biology up to the level of populations. A deeper understanding of microbial responses to stress is indeed attainable only considering the phenomenon as a whole...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27884748/isolation-of-bacterial-strains-able-to-degrade-biphenyl-diphenyl-ether-and-the-heat-transfer-fluid-used-in-thermo-solar-plants
#19
Rafael Blanco-Moreno, Lara P Sáez, Víctor M Luque-Almagro, M Dolores Roldán, Conrado Moreno-Vivián
Thermo-solar plants use eutectic mixtures of diphenyl ether (DE) and biphenyl (BP) as heat transfer fluid (HTF). Potential losses of HTF may contaminate soils and bioremediation is an attractive tool for its treatment. DE- or BP-degrading bacteria are known, but up to now bacteria able to degrade HTF mixture have not been described. Here, five bacterial strains which are able to grow with HTF or its separate components DE and BP as sole carbon sources have been isolated, either from soils exposed to HTF or from rhizospheric soils of plants growing near a thermo-solar plant...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27810336/clinical-scale-expansion-of-cd34-cord-blood-cells-amplifies-committed-progenitors-and-rapid-scid-repopulation-cells
#20
Alba Casamayor-Genescà, Arnau Pla, Irene Oliver-Vila, Noèlia Pujals-Fonts, Sílvia Marín-Gallén, Marta Caminal, Irma Pujol-Autonell, Jorge Carrascal, Marta Vives-Pi, Joan Garcia, Joaquim Vives
Umbilical cord blood (UCB) transplantation is associated with long periods of aplastic anaemia. This undesirable situation is due to the low cell dose available per unit of UCB and the immaturity of its progenitors. To overcome this, we present a cell culture strategy aimed at the expansion of the CD34(+) population and the generation of granulocyte lineage-committed progenitors. Two culture products were produced after either 6 or 14days of in vitro expansion, and their characteristics compared to non-expanded UCB CD34(+) controls in terms of phenotype, colony-forming activity and multilineage repopulation potential in NOD-scid IL2Rγ(null) mice...
March 25, 2017: New Biotechnology
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