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New Biotechnology

Manoj G Kulkarni, Kannan R R Rengasamy, Srinivasa C Pendota, Jiří Gruz, Lenka Plačková, Ondřej Novák, Karel Doležal, Johannes Van Staden
Bioactive compounds such as karrikinolide (KAR1 from smoke) and eckol (from the seaweed Ecklonia maxima) show promising effects on several important crop plants. These plant growth-stimulating organic biomolecules, along with crude extracts (smoke-water and Kelpak® product prepared from Ecklonia maxima), were tested on spinach plants. Eckol sprayed at 10-6 M significantly increased all the growth and biochemical parameters examined compared to control spinach plants. All tested plant growth biostimulants significantly increased total chlorophyll, carotenoids and protein content of spinach leaves...
August 8, 2018: New Biotechnology
Jan Christoph Peinemann, Lina Maja Maria Krenz, Daniel Pleissner
This communication elaborates on the use of seashell powder (SP) for the removal of phosphate from lactic acid-containing fermentation broth. Despite extensive past research regarding the application of SP for phosphate removal from wastewater, no information is available for solutions containing various organic compounds. In order to fill this knowledge gap, tests were performed with pure phosphate solution (PPS) and PPS containing 0.83 M of three alcohols, ethanol, propanol or 1,2-propanediol, or 0.83 M of three organic acids, acetic, propionic or lactic acid...
August 8, 2018: New Biotechnology
Michael J Taussig, Cláudia Fonseca, James S Trimmer
Validation of antibodies and other protein binders is a subject of pressing concern for the research community and one which is uppermost in the minds of all who use antibodies as research and diagnostic reagents. Assessing an antibody's fitness for purpose includes accurate ascertainment of its target specificity and suitability for the envisaged task. Moreover, standardised procedures are essential to guarantee sample quality in testing procedures. The problem of defining precise standards for antibody validation has engendered much debate in recent publications and meetings, but gradually a consensus is emerging...
August 4, 2018: New Biotechnology
Adéla Hýlová, Tomáš Pospíšil, Lukáš Spíchal, Jurgen J Mateman, Daniel Blanco-Ania, Binne Zwanenburg
Strigolactones (SLs) constitute a new class of plant hormones of increasing importance in plant science. The structure of natural SLs is too complex for ready access by synthesis. Therefore, much attention is being given to design of SL analogues and mimics with a simpler structure but with retention of bioactivity. Here new hybrid type SL mimics have been designed derived from auxins, the common plant growth regulators. Auxins were simply coupled with the butenolide D-ring using bromo (or chloro) butenolide...
August 2, 2018: New Biotechnology
A da Costa, A M Pereira, A C Gomes, J C Rodriguez-Cabello, M Casal, R Machado
With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purification are expensive and time consuming. Here, we describe the design, production, purification and assessment of the antibacterial activity of the human peptide hepcidin, using an elastin-like recombinamer as fusion partner...
July 25, 2018: New Biotechnology
Aneta Strejckova, Marek Dvorak, Borivoj Klejdus, Olga Krystofova, Josef Hedbavny, Vojtech Adam, Dalibor Huska
This work has analysed the influence of CdCl2 , NiCl2 and CuCl2 on simple phenolic acids, such as the 3PPP (phenylpyruvic, phenylacetic, and 4-hydroxyphenylpyruvic) and 2DR (3,4-dihydroxyphenylacetic and rosmarinic) phenolic acids for the first time and studied their interactions with antioxidant systems and the glutathione-ascorbate cycle in the freshwater green microalga Scenedesmus quadricauda. The compounds investigated are related to both the catabolic and anabolic pathways of L-phenylalanine and L-tyrosine, the main molecules in the biosynthesis of polyphenols...
July 23, 2018: New Biotechnology
Kateřina Podlešáková, Lydia Ugena, Lukáš Spíchal, Karel Doležal, Nuria De Diego
In plants, γ-aminobutyric acid (GABA) accumulates rapidly in response to environmental stress and variations in its endogenous concentration have been shown to affect plant growth. Exogenous application of GABA has also conferred higher stress tolerance by modulating the expression of genes involved in plant signalling, transcriptional regulation, hormone biosynthesis, reactive oxygen species production and polyamine metabolism. Plant hormones play critical roles in adaptation of plants to adverse environmental conditions through a sophisticated crosstalk among them...
July 23, 2018: New Biotechnology
María Hijosa-Valsero, Ana I Paniagua-García, Rebeca Díez-Antolínez
Potato peel from a snack factory was assessed as possible feedstock for biobutanol production. This lignocellulosic biomass was subjected to various physicochemical pretreatments (autohydrolysis and hydrolysis with dilute acids, alkalis, organic solvents or surfactants) under different conditions of time, temperature and reagent concentrations, in order to favour the release of sugars and reduce the generation of fermentation inhibitors. Thereafter, the pretreated potato peel was treated enzymatically to complete the hydrolysis...
July 22, 2018: New Biotechnology
Žnidaršič-Plazl Polona, Zelić Bruno
No abstract text is available yet for this article.
June 28, 2018: New Biotechnology
Mariamichela Lanzilli, Giuliana Donadio, Francesca Anna Fusco, Carmen Sarcinelli, Danila Limauro, Ezio Ricca, Rachele Isticato
Bacterial spores displaying heterologous proteins have been proposed as a safe and efficient method for delivery of antigens and enzymes to animal mucosal surfaces. Initial studies have been performed using Bacillus subtilis spores, but other spore forming organisms have also been considered. B. megaterium spores have been shown capable of displaying large amounts of a model heterologous protein (Discosoma red fluorescent protein mRFP) that in part crossed the exosporium to localize in the space between the outer coat layer and the exosporium...
June 27, 2018: New Biotechnology
Kristýna Bieleszová, Barbora Pařízková, Martin Kubeš, Alexandra Husičková, Martin Kubala, Qian Ma, Michaela Sedlářová, Stéphanie Robert, Karel Doležal, Miroslav Strnad, Ondřej Novák, Asta Žukauskaitė
The plant hormone auxin is a key player in the regulation of plant growth and development. Despite numerous studies devoted to understanding its role in a wide spectrum of physiological processes, full appreciation of its function is linked to a comprehensive determination of its spatio-temporal distribution, which plays a crucial role in its mode of action. Conjugation of fluorescent tracers to plant hormones enables sensitive and specific visualization of their subcellular and tissue-specific localization and transport in planta, which represents a powerful tool for plant physiology...
June 25, 2018: New Biotechnology
Éric Dumont
In order to limit ammonia (NH3 ) emissions from pig farms, various air cleaning solutions are widely applied. However, the literature data report that these systems (chemical scrubbers, bioscrubbers and biofilters) can be both inefficient and promote nitrous oxide (N2 O) production. As air cleaning technologies should not contribute to secondary trace gases that may have a stronger environmental impact than the raw gas compounds themselves, the objective of this study was to quantify the effect of NH3 treatment in pig farms on greenhouse gas (GHG) emissions...
June 19, 2018: New Biotechnology
Gabriela Kalčíková, Gregor Marolt, Anita Jemec Kokalj, Andreja Žgajnar Gotvajn
Extensive production of nanomaterials of various properties needs to be coupled with rapid toxicity testing in order to provide information about their potential risks to the environment and human health. Miniaturization of toxicity tests may accelerate economical testing of nanomaterials, but is not a common practice. We describe a case study to miniaturize a commonly used toxicity test with plant duckweed Lemna minor. 6-well, 12-well and 24-well culture plates were used to assess their potential use for the duckweed toxicity test with potassium chloride as reference material...
June 15, 2018: New Biotechnology
Nagesh K Tripathi, Divyanshi Karothia, Ambuj Shrivastava, Swati Banger, Jyoti S Kumar
West Nile virus (WNV) is an emerging mosquito-borne virus which is responsible for severe and fatal encephalitis in humans and for which there is no licensed vaccine or therapeutic available to prevent infection. The envelope domain III protein (EDIII) of WNV was over-expressed in Escherichia coli and purified using a two-step chromatography process which included immobilized metal affinity chromatography and ion exchange chromatography. E. coli cells were grown in a bioreactor to high density using batch and fed-batch cultivation...
June 5, 2018: New Biotechnology
Fortunato Ferrara, Martin Kolnik, Sara D'Angelo, Frank M Erasmus, Daniela Vorholt, Andrew R M Bradbury
The study of the biology and function of B cells, or the dissection and in vitro creation of enormous recombinant antibody repertoires, requires the isolation of large numbers of pure CD19+ B cells. The StraightFrom® Leukopak CD19 MicroBead Kit was recently introduced as a fast and robust kit to isolate human CD19+ B cells. This uses paramagnetic microbeads conjugated to high-affinity anti-CD19 monoclonal antibodies to bind B cells in leukapheresis (Leukopak) samples. The overall purity of the isolated cells, together with the characterization of the different CD19+ subclasses, was assessed by flow cytometry using a recombinant (REAffinity) antibody panel, revealing that the method allowed the recovery of over 93% of CD19+ B cells without any pre-purification step...
June 2, 2018: New Biotechnology
Alfred Fernández-Castané, Hong Li, Owen R T Thomas, Tim W Overton
The development of a simple pH-stat fed-batch fermentation strategy for the production of Magnetospirillum gryphiswaldense MSR-1 and magnetosomes (nanoscale magnetic organelles with biotechnological applications) is described. Flow cytometry was exploited as a powerful analytical tool for process development, enabling rapid monitoring of cell morphology, physiology and polyhydroxyalkanoate production. The pH-stat fed-batch growth strategy was developed by varying the concentrations of the carbon source (lactic acid) and the alternative electron acceptor (sodium nitrate) in the feed...
June 1, 2018: New Biotechnology
Petr Tarkowski, Ivo Frébort
No abstract text is available yet for this article.
May 26, 2018: New Biotechnology
Ajay Badhan, Jiangli Huang, Yuxi Wang, D Wade Abbott, Marcos Di Falco, Adrian Tsang, Tim McAllister
In the present study, we have characterized high molecular weight multi-enzyme complexes in two commercial enzymes produced by Trichoderma reesei (Spezyme CP) and Penicillium funiculosum (Accellerase XC). We successfully identified 146-1000 kDa complexes using Blue native polyacrylamide gel electrophoresis (BN-PAGE) to fractionate the protein profile in both preparations. Identified complexes dissociated into lower molecular weight constituents when loaded on SDS PAGE. Unfolding of the secondary structure of multi-enzyme complexes with trimethylamine (pH >10) suggested that they were not a result of unspecific protein aggregation...
May 24, 2018: New Biotechnology
Biljana Đorđević, Vilém Neděla, Eva Tihlaříková, Václav Trojan, Ladislav Havel
Somatic embryogenesis is an important biotechnological technique which can be used in studies associated with environmental stress. Four embryogenic cell lines of Norway spruce were grown on media enriched with copper and arsenic in concentration ranges 50-500 μM and 10-50 μM, respectively. The effects were observed during subsequent stages of somatic embryogenesis, the characteristics evaluated being proliferation potential, average number of somatic embryos obtained per g/fresh weight, morphology of developed somatic embryos, metal uptake, and microanalysis of macro- and micronutrients uptake...
May 18, 2018: New Biotechnology
Nataša Miložič, Gorazd Stojkovič, Andreas Vogel, Dominique Bouwes, Polona Žnidaršič-Plazl
The industrial importance of optically pure compounds has thrown a spotlight on ω-transaminases that have shown a high potential for the synthesis of bioactive compounds with a chiral amine moiety. The implementation of biocatalysts in industrial processes relies strongly on fast and cost effective process development, including selection of a biocatalyst form and the strategy for its immobilization. Here, microscale reactors with selected surface-immobilized amine-transaminase (ATA) in various forms are described as platforms for high-throughput process development...
May 18, 2018: New Biotechnology
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