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New Biotechnology

I González-Domínguez, N Grimaldi, L Cervera, N Ventosa, F Gòdia
Polyethyleneimine (PEI) has been used extensively for transient gene expression (TGE) in mammalian cell cultures. However, the relationship between DNA/PEI complex preparation and their biological activity has not been fully established. Here, a systematic study of DNA/PEI complexes, their physicochemical properties during formation and their transfection efficiency was performed on a virus-like particle (VLP) production platform. The same chemically defined cell culture medium for DNA/PEI complex formation was used as an alternative to simple ionic solutions to minimize changes in complex properties during transfection...
October 3, 2018: New Biotechnology
Manuel Neves, Rita Azevedo, Luís Lima, Marta I Oliveira, Andreia Peixoto, Dylan Ferreira, Janine Soares, Elisabete Fernandes, Cristiana Gaiteiro, Carlos Palmeira, Sofia Cotton, Stefan Mereiter, Diana Campos, Luís Pedro Afonso, Ricardo Ribeiro, Avelino Fraga, Ana Tavares, Hélder Mansinho, Eurico Monteiro, Paula A Videira, Paulo P Freitas, Celso A Reis, Lúcio Lara Santos, Lorena Dieguez, José Alexandre Ferreira
Circulating tumour cells (CTCs) originating from a primary tumour, lymph nodes and distant metastases hold great potential for liquid biopsies by providing a molecular fingerprint for disease dissemination and its temporal evolution through the course of disease management. CTC enumeration, classically defined on the basis of surface expression of Epithelial Cell Adhesion Molecule (EpCAM) and absence of the pan-leukocyte marker CD45, has been shown to correlate with clinical outcome. However, existing approaches introduce bias into the subsets of captured CTCs, which may exclude biologically and clinically relevant subpopulations...
September 28, 2018: New Biotechnology
Roberta Fontoura, Daniel J Daroit, Ana Paula F Corrêa, Karla S Moresco, Lucélia Santi, Walter O Beys-da-Silva, John R Yates, José Cláudio F Moreira, Adriano Brandelli
Feather hydrolysates were obtained through submerged cultivation of 50 g/L feathers with Chryseobacterium sp. kr6. Culture supernatants, displaying antioxidant properties, as evaluated by the 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging method, were partially purified by gel-filtration chromatography. Fractions showing scavenging activity were pooled, lyophilized and tested at different concentrations (0.1-1.0 mg/mL) by the total reactive antioxidant potential (TRAP) method, showing promising antioxidant capacities...
September 14, 2018: New Biotechnology
Amber L Dorey, Fabio Giovannercole, Duarte Guerreiro, Catarina Marinho
The 4th Microbial Stress Meeting: from Systems to Moleculesand Back was held in April 2018 in Kinsale, Ireland. The meeting covered five main topics: 1. Stress at the systems and structural level; 2. Responses to osmotic and acid stress; 3. Stress responses in single cells; 4. Stress in host-pathogen interactions; and 5. Biotechnological optimisation of microorganisms through engineering and evolution, over three days. Almost 130 delegates, from 24 countries and both the industrial and academic sectors, attended the meeting, presenting 9 lectures, 28 short talks and 52 posters...
September 6, 2018: New Biotechnology
Jan Barciszewski, Maria A Ciemerych, Tomasz Twardowski
Resolution of old problems with new tools seems to be a new way of challenging and finding the keys to innovation. A holistic view of different branches of science and industry, including services for society, is critically important for the development of modern science. The International Congress Eurobiotech 2017 was a special opportunity for such a universal view. In this paper, we discuss the application of different small RNAs, stem cells, epigenetics and sequencing, as well as art and bioeconomy. Our most significant message is not a new one but is very universal: biotechnology is vital for society...
September 5, 2018: New Biotechnology
Mingxin Guo, Weiping Song
Bioeconomy refers to economic activities using renewable biological resources to produce energy and domestic consumables. The U.S. bioeconomy emerged in the early 2000s as a result of the societal pursuit of energy independence and security, greenhouse gas emission mitigation and sustainable development. Founded on biotechnologies and stimulated by federal policies, the U.S. bioeconomy has been growing rapidly over the past decade. Intensive research and development endeavors have been carried out to explore advanced technologies for efficient biomass production, conversion, and valorization, through which numerous biotechnological innovations have been achieved to realize the commercial, cost-effective production of diverse transportation biofuels and high-value domestic bioproducts from various biomass feedstocks...
August 16, 2018: New Biotechnology
Kristi Budzinski, Megan Blewis, Philip Dahlin, Daniel D'Aquila, Julia Esparza, Jack Gavin, Sa V Ho, Clarice Hutchens, David Kahn, Stefan G Koenig, Robert Kottmeier, Jeff Millard, Matt Snyder, Brad Stanard, Lixin Sun
Biopharmaceuticals (or biologics), large molecule therapeutics typically produced using biotechnology, are a rapidly growing segment of the pharmaceutical market. As such, the environmental footprint of the production of these molecules is coming under scrutiny from various stakeholders such as healthcare providers, investors, and even employees. Process mass intensity (PMI), originally adopted for small molecules by the Green Chemistry Institute Pharmaceutical Roundtable, is a simple metric that can also be applied to evaluate the process efficiency of biopharmaceutical production...
August 16, 2018: New Biotechnology
Manoj G Kulkarni, Kannan R R Rengasamy, Srinivasa C Pendota, Jiří Gruz, Lenka Plačková, Ondřej Novák, Karel Doležal, Johannes Van Staden
Bioactive compounds such as karrikinolide (KAR1 from smoke) and eckol (from the seaweed Ecklonia maxima) show promising effects on several important crop plants. These plant growth-stimulating organic biomolecules, along with crude extracts (smoke-water and Kelpak® product prepared from Ecklonia maxima), were tested on spinach plants. Eckol sprayed at 10-6  M significantly increased all the growth and biochemical parameters examined compared to control spinach plants. All tested plant growth biostimulants significantly increased total chlorophyll, carotenoids and protein content of spinach leaves...
August 9, 2018: New Biotechnology
Jan Christoph Peinemann, Lina Maja Maria Krenz, Daniel Pleissner
This communication elaborates on the use of seashell powder (SP) for the removal of phosphate from lactic acid-containing fermentation broth. Despite extensive past research regarding the application of SP for phosphate removal from wastewater, no information is available for solutions containing various organic compounds. In order to fill this knowledge gap, tests were performed with pure phosphate solution (PPS) and PPS containing 0.83 M of three alcohols, ethanol, propanol or 1,2-propanediol, or 0.83 M of three organic acids, acetic, propionic or lactic acid...
August 8, 2018: New Biotechnology
Adéla Hýlová, Tomáš Pospíšil, Lukáš Spíchal, Jurgen J Mateman, Daniel Blanco-Ania, Binne Zwanenburg
Strigolactones (SLs) constitute a new class of plant hormones of increasing importance in plant science. The structure of natural SLs is too complex for ready access by synthesis. Therefore, much attention is being given to design of SL analogues and mimics with a simpler structure but with retention of bioactivity. Here new hybrid type SL mimics have been designed derived from auxins, the common plant growth regulators. Auxins were simply coupled with the butenolide D-ring using bromo (or chloro) butenolide...
August 2, 2018: New Biotechnology
Aneta Strejckova, Marek Dvorak, Borivoj Klejdus, Olga Krystofova, Josef Hedbavny, Vojtech Adam, Dalibor Huska
This work has analysed the influence of CdCl2 , NiCl2 and CuCl2 on simple phenolic acids, such as the 3PPP (phenylpyruvic, phenylacetic, and 4-hydroxyphenylpyruvic) and 2DR (3,4-dihydroxyphenylacetic and rosmarinic) phenolic acids for the first time and studied their interactions with antioxidant systems and the glutathione-ascorbate cycle in the freshwater green microalga Scenedesmus quadricauda. The compounds investigated are related to both the catabolic and anabolic pathways of l-phenylalanine and l-tyrosine, the main molecules in the biosynthesis of polyphenols...
July 23, 2018: New Biotechnology
Kateřina Podlešáková, Lydia Ugena, Lukáš Spíchal, Karel Doležal, Nuria De Diego
In plants, γ-aminobutyric acid (GABA) accumulates rapidly in response to environmental stress and variations in its endogenous concentration have been shown to affect plant growth. Exogenous application of GABA has also conferred higher stress tolerance by modulating the expression of genes involved in plant signalling, transcriptional regulation, hormone biosynthesis, reactive oxygen species production and polyamine metabolism. Plant hormones play critical roles in adaptation of plants to adverse environmental conditions through a sophisticated crosstalk among them...
July 23, 2018: New Biotechnology
Žnidaršič-Plazl Polona, Zelić Bruno
No abstract text is available yet for this article.
December 25, 2018: New Biotechnology
Gabriela Kalčíková, Gregor Marolt, Anita Jemec Kokalj, Andreja Žgajnar Gotvajn
Extensive production of nanomaterials of various properties needs to be coupled with rapid toxicity testing in order to provide information about their potential risks to the environment and human health. Miniaturization of toxicity tests may accelerate economical testing of nanomaterials, but is not a common practice. We describe a case study to miniaturize a commonly used toxicity test with plant duckweed Lemna minor. 6-well, 12-well and 24-well culture plates were used to assess their potential use for the duckweed toxicity test with potassium chloride as reference material...
December 25, 2018: New Biotechnology
Nataša Miložič, Gorazd Stojkovič, Andreas Vogel, Dominique Bouwes, Polona Žnidaršič-Plazl
The industrial importance of optically pure compounds has thrown a spotlight on ω-transaminases that have shown a high potential for the synthesis of bioactive compounds with a chiral amine moiety. The implementation of biocatalysts in industrial processes relies strongly on fast and cost effective process development, including selection of a biocatalyst form and the strategy for its immobilization. Here, microscale reactors with selected surface-immobilized amine-transaminase (ATA) in various forms are described as platforms for high-throughput process development...
December 25, 2018: New Biotechnology
Ana C Fernandes, Benjamin Petersen, Lars Møller, Krist V Gernaey, Ulrich Krühne
The need for fast and comprehensive characterization of biocatalysts has pushed the development of new screening platforms based on microfluidics, capable of monitoring several parameters simultaneously, with new configurations of liquid handling, sample treatment and sensing. Modular microfluidics allows the integration of these newly developed approaches in a more flexible way towards increasing applicability of the microfluidic chips to different types of biocatalysts and reactions. A highly relevant operation in such a system is biocatalyst inactivation, which can enable the precise control of reaction time by avoiding the continuation of the reaction in another module or connecting tubes...
December 25, 2018: New Biotechnology
Lucie Vobecká, Alexandr Romanov, Zdeněk Slouka, Pavel Hasal, Michal Přibyl
Aqueous two-phase systems (ATPSs) were screened for the production of 6-aminopenicillanic acid (6-APA) catalyzed by penicillin acylase, followed by the extractive separation of 6-APA from the reaction mixture. The key point of this study was to find an ATPS exhibiting a large difference in the partition coefficients of the biocatalyst and reaction products. Several ATPSs based on polyethylene glycol (PEG)/phosphate, PEG/citrate, and PEG/dextran were tested. We found that an ATPS consisting of 15 wt% of PEG 4000, 10 wt% of phosphates, 75 wt% of water (pH value 8...
December 25, 2018: New Biotechnology
Eugen Kaganovitch, Xenia Steurer, Deniz Dogan, Christopher Probst, Wolfgang Wiechert, Dietrich Kohlheyer
Microfluidics has enabled various research projects in the field of microbial single-cell analysis. In particular, single-use microfluidic cultivation devices combined with automated time-lapse imaging provide powerful approaches for analyzing microbial phenomena at the single-cell level. High spatiotemporal resolution facilitates individual cell identification and tracking, delivering detailed insights into areas like phenotypic population heterogeneity, which can be highly relevant to the fate of a microbial population and may negatively impact the efficiency of biotechnological fermentations...
December 25, 2018: New Biotechnology
Ana C Fernandes, Daria Semenova, Peter Panjan, Adama M Sesay, Krist V Gernaey, Ulrich Krühne
The limited availability of metabolite-specific sensors for continuous sampling and monitoring is one of the main bottlenecks contributing to failures in bioprocess development. Furthermore, only a limited number of approaches exist to connect currently available measurement systems with high throughput reactor units. This is especially relevant in the biocatalyst screening and characterization stage of process development. In this work, a strategy for sensor integration in microfluidic platforms is demonstrated, to address the need for rapid, cost-effective and high-throughput screening in bioprocesses...
December 25, 2018: New Biotechnology
Saša Vrhovec Hartman, Bojan Božič, Jure Derganc
Microcavities provide a well-controlled flow-free microenvironment and play an important role in many microfluidic systems, for example as cell-culturing microchambers. Here we show that transient concentration gradients that emerge during diffusive exchange of solutes in microcavities induce passive migration (diffusiophoresis) of blood cells and synthetic phospholipid vesicles. The passive migration is observed in various concentration gradients comprising non-electrolytes and electrolytes, i.e., glucose, sucrose, sodium chloride, potassium chloride, potassium benzoate, and potassium sulfate...
December 25, 2018: New Biotechnology
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