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New Biotechnology

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https://www.readbyqxmd.com/read/28315468/in-memory-of-professor-brian-frederic-carl-clark-contributions-from-friends
#1
EDITORIAL
Francesco Lescai, Børge Diderichsen, Marc Van Montagu, Jeffrey Cole
No abstract text is available yet for this article.
March 14, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28286167/fermentative-production-of-butanol-perspectives-on-synthetic-biology
#2
REVIEW
Sonil Nanda, Dasantila Golemi-Kotra, John C McDermott, Ajay K Dalai, Iskender Gökalp, Janusz A Kozinski
Apprehensions relating to global warming, climate change, pollution, rising energy demands as well as fluctuating crude oil prices and supply are leading to a shift in global interest to find suitable alternatives to fossil fuels. This review aims to highlight the many different facets of biobutanol as an advanced next-generation transportation biofuel. Butanol has fuel properties almost on a par with gasoline, such as high energy content, low vapour pressure, non-hygroscopic nature, less volatility, flexible fuel blends and high octane number...
March 9, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28285179/expanding-the-feruloyl-esterase-gene-family-of-aspergillus-niger-by-characterization-of-a-feruloyl-esterase-faec
#3
Adiphol Dilokpimol, Miia R Mäkelä, Sadegh Mansouri, Olga Belova, Martin Waterstraat, Mirko Bunzel, Ronald P de Vries, Kristiina S Hildén
A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10mg/L. FaeC was most active at pH 7.0 and 50°C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme released both ferulic acid and p-coumaric acid from wheat arabinoxylan and sugar beet pectin (up to 3mg/g polysaccharide), and acted synergistically with a commercial xylanase increasing the release of ferulic acid up to six-fold...
March 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28274691/anti-ageing-properties-of-khelma-longevity%C3%A2-treatment-of-human-fibroblasts-increases-proteasome-levels-and-decreases-the-levels-of-oxidized-proteins
#4
Konstantinos Voutetakis, Vasiliki Delitsikou, Michel Georges Magouritsas, Efstathios S Gonos
We have determined the putative anti-ageing properties of Khelma Longevity™, a formula based on various natural compounds from the Mediterranean area. Human primary fibroblast cultures were treated with a wide range of concentrations of Khelma Longevity™ for 1 day or 3 consecutive days. Following these treatments, two major and complementary biomarkers of ageing were measured, namely, the proteasome and the amount of oxidized proteins. It was observed that 24h of treatment with Khelma Longevity™ resulted in a maximum increase of about 41% of the total protein levels of 20S proteasome...
March 6, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28263898/natural-sorbents-modified-by-divalent-cu-2-and-zn-2-ions-and-their-corresponding-antimicrobial-activity
#5
Maja B Đolić, Vladana N Rajaković-Ognjanović, Svetlana B Štrbac, Suzana I Dimitrijević, Miodrag N Mitrić, Antonije E Onjia, Ljubinka V Rajaković
The objective of this study was to investigate the modification of materials used in wastewater treatment for possible antimicrobial application(s). Granulated activated carbon (GAC) and natural clinoptilolite (CLI) were activated using Cu(2+)- and Zn(2+)- ions and the disinfection ability of the resulting materials was tested. Studies of the sorption and desorption kinetics were performed in order to determine and clarify the antimicrobial activity of the metal-activated sorbents. The exact sorption capacities of the selected sorbents, GAC and CLI, activated through use of Cu(2+)- ions, were 15...
March 2, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28212869/improvement-of-simultaneous-cr-vi-and-phenol-removal-by-an-immobilised-bacterial-consortium-and-characterisation-of-biodegradation-products
#6
Ornella M Ontañon, Paola S González, Germán G Barros, Elizabeth Agostini
Microbial bioremediation emerged some decades ago as an eco-friendly technology to restore polluted sites. Traditionally, the search for microorganisms suitable for bioremediation has been based on the selection of isolated strains able to remove a specific type of pollutant. However, this strategy has now become obsolete, since co-pollution is a global reality. Thus, current studies attempt to find bacterial cultures capable of coping with a mixture of organic and inorganic compounds. In this sense, the bacterial consortium SFC 500-1 has demonstrated efficiency for Cr(VI) and phenol removal, both of which are found in many industrial wastewaters...
February 16, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28188935/model-based-characterisation-of-growth-performance-and-l-lactic-acid-production-with-high-optical-purity-by-thermophilic-bacillus-coagulans-in-a-lignin-supplemented-mixed-substrate-medium
#7
Robert Glaser, Joachim Venus
Three Bacillus coagulans strains were characterised in terms of their ability to grow in lignin-containing fermentation media and to consume the lignocellulose-related sugars glucose, xylose, and arabinose. An optical-density high-throughput screening was used for precharacterisation by means of different mathematical models for comparison (Logistic, Gompertz, Baranyi, Richards & Stannard, and Schnute). The growth response was characterised by the maximum growth rate and lag time. For a comparison of the screening and fermentation results, an unstructured mathematical model was proposed to characterise the lactate production, bacterial growth and substrate consumption...
February 8, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28188936/cultivation-of-podospora-anserina-on-soybean-hulls-results-in-an-efficient-enzyme-cocktail-for-plant-biomass-hydrolysis
#8
Miia R Mäkelä, Ourdia Bouzid, Diogo Robl, Harm Post, Mao Peng, Albert Heck, Maarten Altelaar, Ronald P de Vries
The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures...
February 7, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28179151/development-of-efficient-adeno-associated-virus-aav-mediated-gene-delivery-system-with-a-phytoactive-material-for-targeting-human-melanoma-cells
#9
John Hwan Lee, Yoojin Kim, Ye-Eun Yoon, Yong-Jin Kim, Seong-Geun Oh, Jae-Hyung Jang, Eunmi Kim
We exploited the emerging potential of gene therapy strategies to design a powerful therapeutic system that combines two key components-AAV vector and [6]-gingerol. In this study, we created an AAV2 construct expressing the proapoptotic protein BIM, which uses HSPG as its primary receptor, to target HSPG-overexpressing melanoma cells. This combination treatment showed promising results in vitro, inducing apoptosis in human melanoma cells. This new platform technology will make a significant contribution to numerous therapeutic applications, most notably for melanoma, including overcoming resistance to conventional anticancer therapies...
February 5, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28159727/a-p-coumaroyl-esterase-from-rhizoctonia-solani-with-a-pronounced-chlorogenic-acid-esterase-activity
#10
Annabel Nieter, Sebastian Kelle, Diana Linke, Ralf G Berger
Extracellular esterase activity was detected in submerged cultures of Rhizoctonia solani grown in the presence of sugar beet pectin or Tween 80. Putative type B feruloyl esterase (FAE) coding sequences found in the genome data of the basidiomycete were heterologously expressed in Pichia pastoris. Recombinant enzyme production on the 5-L bioreactor scale (Rs pCAE: 3245UL(-1)) exceeded the productivity of the wild type strain by a factor of 800. Based on substrate specificity profiling, the purified recombinant Rs pCAE was classified as a p-coumaroyl esterase (pCAE) with a pronounced chlorogenic acid esterase side activity...
January 31, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28137467/engineering-bispecific-antibodies-with-defined-chain-pairing
#11
Simon Krah, Carolin Sellmann, Laura Rhiel, Christian Schröter, Stephan Dickgießer, Jan Beck, Stefan Zielonka, Lars Toleikis, Björn Hock, Harald Kolmar, Stefan Becker
Bispecific IgG-like antibodies can simultaneously interact with two epitopes on the same or on different antigens. Therefore, these molecules facilitate novel modes of action, which cannot be addressed by conventional monospecific IgGs. However, the generation of such antibodies still appears to be demanding due to their specific architecture comprising four different polypeptide chains that need to assemble correctly. This review focusses on different strategies to circumvent this issue or to enforce a correct chain association with a focus on common-chain bispecific antibodies...
January 27, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28284327/special-issue-of-new-biotechnology-biopolymers-eu-symposium
#12
EDITORIAL
Mauro Majone, Martin Koller, Marianna Villano
No abstract text is available yet for this article.
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27903429/polyhydroxyalkanoate-as-a-slow-release-carbon-source-for-in-situ-bioremediation-of-contaminated-aquifers-from-laboratory-investigation-to-pilot-scale-testing-in-the-field
#13
Lucia Pierro, Bruna Matturro, Simona Rossetti, Marco Sagliaschi, Salvatore Sucato, Eduard Alesi, Ernst Bartsch, Firoozeh Arjmand, Marco Petrangeli Papini
A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs)...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27793692/strategies-for-efficiently-selecting-pha-producing-mixed-microbial-cultures-using-complex-feedstocks-feast-and-famine-regime-and-uncoupled-carbon-and-nitrogen-availabilities
#14
Catarina S S Oliveira, Carlos E Silva, Gilda Carvalho, Maria A Reis
Production of polyhydroxyalkanoates (PHAs) by open mixed microbial cultures (MMCs) has been attracting increasing interest as an alternative technology to PHA production by pure cultures, due to the potential for lower costs associated with the use of open systems (eliminating the requirement for sterile conditions) and the utilisation of cheap feedstock (industrial and agricultural wastes). Such technology relies on the efficient selection of an MMC enriched in PHA-accumulating organisms. Fermented cheese whey, a protein-rich complex feedstock, has been used previously to produce PHA using the feast and famine regime for selection of PHA accumulating cultures...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27593394/new-insights-into-pseudomonas-fluorescens-alginate-biosynthesis-relevant-for-the-establishment-of-an-efficient-production-process-for-microbial-alginates
#15
REVIEW
Susan Maleki, Mali Mærk, Radka Hrudikova, Svein Valla, Helga Ertesvåg
Alginate denotes a family of linear polysaccharides with a wide range of industrial and pharmaceutical applications. Presently, all commercially available alginates are manufactured from brown algae. However, bacterial alginates have advantages with regard to compositional homogeneity and reproducibility. In order to be able to design bacterial strains that are better suited for industrial alginate production, defining limiting factors for alginate biosynthesis is of vital importance. Our group has been studying alginate biosynthesis in Pseudomonas fluorescens using several complementary approaches...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27576102/hyaluronan-cholesterol-nanohydrogels-characterisation-and-effectiveness-in-carrying-alginate-lyase
#16
Elita Montanari, Chiara Di Meo, Simona Sennato, Antonio Francioso, Anna Laura Marinelli, Francesca Ranzo, Serena Schippa, Tommasina Coviello, Federico Bordi, Pietro Matricardi
Although in recent years several methods have been studied and developed to obtain different types of nanosized drug delivery systems, the set up of suitable procedures and materials remains highly expensive, their preparation is time consuming and often not feasible for a scale-up process. Furthermore, the sterilisation and storage of nanocarrier formulations represents a complicated but mandatory step for their effective use. In our previous work we assessed the use of an autoclaving process to achieve, in one simple step, sterile self-assembled hyaluronan-cholesterol (HA-CH) and hyaluronan-riboflavin (HA-Rfv) nanohydrogels (NHs)...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27457131/impact-of-nitrogen-feeding-regulation-on-polyhydroxyalkanoates-production-by-mixed-microbial-cultures
#17
Fernando Silva, Sabrina Campanari, Stefania Matteo, Francesco Valentino, Mauro Majone, Marianna Villano
A sequencing batch reactor (SBR) is typically used for selecting mixed microbial cultures (MMC) for polyhydroxyalkanoate (PHA) production. Since many waste streams suitable as process feedstock for PHA production are nitrogen-deficient, a nutrient supply in the SBR is typically required to allow for efficient microbial growth. The scope of this study was to devise a nitrogen feeding strategy which allows controlling the nitrogen levels during the feast and famine regime of a lab-scale SBR, thereby selecting for PHA-storing microorganisms...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27445201/replacing-fossil-based-plastic-performance-products-by-bio-based-plastic-products-technical-feasibility
#18
Martien van den Oever, Karin Molenveld
Larger scale market introduction of new bio-based products requires a clear advantage regarding sustainability, as well as an adequate techno-economic positioning relative to fossil based products. In a previous paper [Broeren et al., 2016], LCA results per kg and per functionality equivalent of bio-based plastics were presented, together with economic considerations. The present paper discusses the mechanical and thermal properties of a range of commercially available bio-based plastics based on polylactic acid (PLA), cellulose esters, starch and polyamides, and the feasibility of replacing fossil-based counterparts based on performance...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27445200/transcriptome-analysis-of-pseudomonas-mediterranea-and-p-corrugata-plant-pathogens-during-accumulation-of-medium-chain-length-phas-by-glycerol-bioconversion
#19
Grazia Licciardello, Rosario Ferraro, Marcella Russo, Francesco Strozzi, Antonino F Catara, Patrizia Bella, Vittoria Catara
Pseudomonas corrugata and P. mediterranea are soil inhabitant bacteria, generally living as endophytes on symptomless plants and bare soil, but also capable of causing plant diseases. They share a similar genome size and a high proteome similarity. P. corrugata produces many biomolecules which play an important role in bacterial cell survival and fitness. Both species produce different medium-chain-length PHAs (mcl-PHAs) from the bioconversion of glycerol to a transparent film in P. mediterranea and a sticky elastomer in P...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27432196/self-assembly-and-drug-release-study-of-linear-l-d-oligopeptide-poly-ethylene-glycol-conjugates
#20
Federica Novelli, Serena De Santis, Pasqualina Punzi, Cesare Giordano, Anita Scipioni, Giancarlo Masci
The preparation and structural organisation of new bioinspired nanomaterials based on regular alternating enantiomeric sequence of tetra- and hexapeptides end-linked to poly(ethylene glycol) (PEG) is reported. The peptide moiety is composed of two or three repeats of l-Ala-d-Val units while the PEG has a molecular weight of 2kDa. The self-assembling properties of the two conjugates depend significantly on the length of the peptide. Nanoparticles with different sizes and morphologies are formed, the structural properties of which are compared with the previously studied l-Ala-d-Val octapeptide conjugate that self-assembles into rod-like nanoparticles...
July 25, 2017: New Biotechnology
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