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New Biotechnology

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https://www.readbyqxmd.com/read/28212869/improvement-of-simultaneous-cr-vi-and-phenol-removal-by-an-immobilised-bacterial-consortium-and-characterisation-of-biodegradation-products
#1
Ornella M Ontañon, Paola S González, Germán Barros, Elizabeth Agostini
Microbial bioremediation emerged some decades ago as an eco-friendly technology to restore polluted sites. Traditionally, the search for microorganisms suitable for bioremediation has been based on the selection of isolated strains able to remove a specific type of pollutant. However, this strategy has now become obsolete, since co-pollution is a global reality. Thus, current studies attempt to find bacterial cultures capable of coping with a mixture of organic and inorganic compounds. In this sense, the bacterial consortium SFC 500-1 has demonstrated efficiency for Cr(VI) and phenol removal, both of which are found in many industrial wastewaters...
February 14, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28188936/cultivation-of-podospora-anserina-on-soybean-hulls-results-in-an-efficient-enzyme-cocktail-for-plant-biomass-hydrolysis
#2
Miia R Mäkelä, Ourdia Bouzid, Diogo Robl, Harm Post, Mao Peng, Albert Heck, Maarten Altelaar, Ronald P de Vries
The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures...
February 7, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28188935/model-based-characterisation-of-growth-performance-and-l-lactic-acid-production-with-high-optical-purity-by-thermophilic-bacillus-coagulans-in-a-lignin-supplemented-mixed-substrate-medium
#3
Robert Glaser, Joachim Venus
Three Bacillus coagulans strains were characterised in terms of their ability to grow in lignin-containing fermentation media and to consume the lignocellulose-related sugars glucose, xylose, and arabinose. An optical-density high-throughput screening was used for precharacterisation by means of different mathematical models for comparison (Logistic, Gompertz, Baranyi, Richards & Stannard, and Schnute). The growth response was characterised by the maximum growth rate and lag time. For a comparison of the screening and fermentation results, an unstructured mathematical model was proposed to characterise the lactate production, bacterial growth and substrate consumption...
February 7, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28179151/development-of-efficient-adeno-associated-virus-aav-mediated-gene-delivery-system-with-a-phytoactive-material-for-targeting-human-melanoma-cells
#4
John Hwan Lee, Yoojin Kim, Ye-Eun Yoon, Yong-Jin Kim, Seong-Geun Oh, Jae-Hyung Jang, Eunmi Kim
No abstract text is available yet for this article.
February 4, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28159727/a-p-coumaroyl-esterase-from-rhizoctonia-solani-with-a-pronounced-chlorogenic-acid-esterase-activity
#5
Annabel Nieter, Sebastian Kelle, Diana Linke, Ralf G Berger
Extracellular esterase activity was detected in submerged cultures of Rhizoctonia solani grown in the presence of sugar beet pectin or Tween 80. Putative type B feruloyl esterase (FAE) coding sequences found in the genome data of the basidiomycete were heterologously expressed in Pichia pastoris. Recombinant enzyme production on the 5-L bioreactor scale (RspCAE: 3245UL(-1)) exceeded the productivity of the wild type strain by a factor of 800. Based on substrate specificity profiling, the purified recombinant RspCAE was classified as a p-coumaroyl esterase (pCAE) with a pronounced chlorogenic acid esterase side activity...
January 31, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28137467/engineering-bispecific-antibodies-with-defined-chain-pairing
#6
Simon Krah, Carolin Sellmann, Laura Rhiel, Christian Schröter, Stephan Dickgießer, Jan Beck, Stefan Zielonka, Lars Toleikis, Björn Hock, Harald Kolmar, Stefan Becker
Bispecific IgG-like antibodies can simultaneously interact with two epitopes on the same or on different antigens. Therefore, these molecules facilitate novel modes of action, which cannot be addressed by conventional monospecific IgGs. However, the generation of such antibodies still appears to be demanding due to their specific architecture comprising four different polypeptide chains that need to assemble correctly. This review focusses on different strategies to circumvent this issue or to enforce a correct chain association with a focus on common-chain bispecific antibodies...
January 27, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28040555/juncus-spp-the-helophyte-for-all-phyto-remediation-purposes
#7
Evdokia Syranidou, Stavros Christofilopoulos, Nicolas Kalogerakis
Helophytic plants contribute significantly to the remediation of ecosystems through a wide range of physiological or biochemical mechanisms including the role of endophytic bacteria. This review highlights the services provided by Juncus spp. wetland plants, from phytoremediation of soils and groundwater with heavy metals and/or organics to municipal or industrial wastewater treatment in constructed wetlands. The data presented also provide information on the efficiency of specific Juncus spp. in response to various metals and organic compounds, in an effort to exploit the natural capabilities of autochthonous over exotic species in phytoremediation strategies...
December 28, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28024971/in-commemoration-of-completion-of-the-genetic-code-and-in-memory-of-prof-brian-clark
#8
Huanming Yang
While we celebrate the 20th anniversary of the complete decoding of the genetic code, we should also commemorate Prof. Brian Clark for his remarkable contributions to this historic landmark in life sciences, as well as to the global collaboration on sciences. As an international citizen and a great scientist, Prof. Brian Clark will live for ever in the hearts of all his friends and students.
December 23, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28011289/immobilization-of-laccase-of-pycnoporus-sanguineus-cs43
#9
Luis A Gonzalez-Coronel, Marta Cobas, Magdalena de J Rostro-Alanis, Roberto Parra-Saldívar, Carlos Hernandez-Luna, Marta Pazos, M Ángeles Sanromán
Laccase from Pycnoporus sanguineus CS43 was successfully immobilized onto Immobead-150 and Eupergit-C by covalent binding and by entrapment in LentiKats. The highest immobilization was onto Immobead-150 (97.1±1.2%) compared to the other supports, LentiKats (89±1.1%) and Eupergit-C (83.2±1.4%). All three immobilized enzyme systems showed increased thermostability and better mechanical properties than free laccase. Moreover, after 5 cycles of reuse of these systems, 90% of initial laccase activity was retained...
December 21, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27988304/overview-of-the-membrane-associated-ring-ch-march-e3-ligase-family
#10
Johannes Bauer, Oddmund Bakke, J Preben Morth
E3 ligases are critical checkpoints for protein ubiquitination, a signal that often results in protein sorting and degradation but has also been linked to regulation of transcription and DNA repair. In line with their key role in cellular trafficking and cell-cycle control, malfunction of E3 ligases is often linked to human disease. Thus, they have emerged as prime drug targets. However, the molecular basis of action of membrane-bound E3 ligases is still unknown. Here, we review the current knowledge on the membrane-embedded MARCH E3 ligases (MARCH-1-6,7,8,11) with a focus on how the transmembrane regions can contribute via GxxxG-motifs to the selection and recognition of other membrane proteins as substrates for ubiquitination...
December 14, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27915060/special-issue-of-new-biotechnology-in-memory-of-professor-brian-f-c-clark-1936-2014
#11
EDITORIAL
Francesco Lescai
No abstract text is available yet for this article.
November 30, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27903429/polyhydroxyalkanoate-as-a-slow-release-carbon-source-for-in-situ-bioremediation-of-contaminated-aquifers-from-laboratory-investigation-to-pilot-scale-testing-in-the-field
#12
Lucia Pierro, Bruna Matturro, Simona Rossetti, Marco Sagliaschi, Salvatore Sucato, Eduard Alesi, Ernst Bartsch, Firoozeh Arjmand, Marco Petrangeli Papini
A pilot-scale study aiming to evaluate the potential use of poly-3-hydroxy-butyrate (PHB) as an electron donor source for in situ bioremediation of chlorinated hydrocarbons in groundwater was conducted. Compared with commercially available electron donors, PHB offers a restricted fermentation pathway (i.e., through acetic acid and molecular hydrogen) by avoiding the formation of any residual carbon that could potentially spoil groundwater quality. The pilot study was carried out at an industrial site in Italy, heavily contaminated by different chlorinated aliphatic hydrocarbons (CAHs)...
November 27, 2016: New Biotechnology
https://www.readbyqxmd.com/read/28126513/inhibition-of-the-respiratory-chain-reactions-in-denitrifying-ebpr-biomass-under-simultaneous-presence-of-acetate-and-electron-acceptor
#13
Ilias Zafiriadis, Anastasios G Kapagiannidis, Spyridon Ntougias, Alexander Aivasidis
In this study, the deterioration of the typical EBPR (Enhanced Biological Phosphorus Removal) process due to the simultaneous presence of electron donor (external substrate) and electron acceptor (oxygen or nitrate) was investigated by using a PAOs (Polyphosphate Accumulating Organisms)-enriched biomass grown in a modified DEPHANOX system. Intracellular and extracellular constituents were monitored in batch tests under different electron donor and acceptor conditions and specific oxygen and nitrogen uptake rates were evaluated...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28069519/denaturing-fixatives-are-compatible-with-the-nanostring-ncounter-%C3%A2-platform-and-the-prosigna-%C3%A2-assay
#14
COMPARATIVE STUDY
Roman Rouzier, Aurelie Roulot, Arthur H Jeiranian, Namratha Ram, Jean Marc Guinebretiere, Anne Vincent Salomon, David Gentien
The objective of this study was to establish that the denaturing fixative, formalin and acetic acid (AFA), is equivalent to neutral-buffered formalin (NBF) on the nCounter system using the Prosigna assay. Twenty-five previously resected tumors from breast cancer patients were apportioned and fixed with either NBF or AFA prior to embedding in paraffin. Differentially fixed and paraffin embedded tissue pairs were sectioned and pathological review was performed according to the Prosigna assay protocol. RNA was isolated using the Prosigna assay kit and analyzed using the NanoString nCounter Dx Analysis system...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#15
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28043869/isolation-of-rhamnolipids-producing-cultures-from-faeces-influence-of-interspecies-communication-on-the-yield-of-rhamnolipid-congeners
#16
Marta Woźniak-Karczewska, Kamila Myszka, Agata Sznajdrowska, Alicja Szulc, Agnieszka Zgoła-Grześkowiak, Łukasz Ławniczak, Philippe F-X Corvini, Łukasz Chrzanowski
The aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. Initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce biosurfactants...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28043868/evaluation-of-anaerobic-digestates-from-different-feedstocks-as-growth-media-for-tetradesmus-obliquus-botryococcus-braunii-phaeodactylum-tricornutum-and-arthrospira-maxima
#17
Marina Massa, Silvia Buono, Antonio Luca Langellotti, Luigi Castaldo, Anna Martello, Antonello Paduano, Raffaele Sacchi, Vincenzo Fogliano
In this paper, two freshwater microalgae (Tetradesmus obliquus and Botryococcus braunii), a marine diatom (Phaeodactylum tricornutum) and a photosynthetic cyanobacterium (Arthrospira maxima) were investigated for their ability to grow on liquid digestates (LDs). Three LDs were obtained from anaerobic digestion of different organic wastes: zootechnical (ZW LD), vegetable biomass (VW LD) and the organic fraction of municipal solid wastes (MW LD). All the strains showed the same growth performance on VW LD as on the respective standard media (SM), while ZW LD was efficient only for growth of T...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27986600/effect-of-culture-residence-time-on-substrate-uptake-and-storage-by-a-pure-culture-of-thiothrix-ct3-strain-under-continuous-or-batch-feeding
#18
Francesco Valentino, Mario Beccari, Marianna Villano, Valter Tandoi, Mauro Majone
A pure culture of the filamentous bacterium Thiothrix, strain CT3, was aerobically cultured in a chemostat under continuous acetate feeding at three different culture residence times (RT 6, 12 or 22 d) and the same volumetric organic load rate (OLR 0.12gCOD/L/d). Cells cultured at decreasing RT in the chemostat had an increasing transient response to acetate spikes in batch tests. The maximum specific acetate removal rate increased from 25 to 185mgCOD/gCOD/h, corresponding to a 1.8 to 8.1 fold higher respective steady-state rate in the chemostat...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27989956/pseudoalteromonas-haloplanktis-produces-methylamine-a-volatile-compound-active-against-burkholderia-cepacia-complex-strains
#19
Filomena Sannino, Ermenegilda Parrilli, Gennaro Antonio Apuzzo, Donatella de Pascale, Pietro Tedesco, Isabel Maida, Elena Perrin, Marco Fondi, Renato Fani, Gennaro Marino, Maria Luisa Tutino
The Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125 has been reported to produce several Volatile Organic Compounds (VOCs), which are able to inhibit the growth of Burkholderia cepacia complex (Bcc) strains, opportunistic pathogens responsible for the infection of immune-compromised patients. However, no specific antibacterial VOCs have been identified to date. The purpose of the present study was to identify specific VOCs that contribute to Bcc inhibition by the Antarctic strain. When grown on defined medium containing D-gluconate and L-glutamate as carbon, nitrogen and energy sources, P...
March 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27932313/3rd-congress-on-applied-synthetic-biology-in-europe-costa-da-caparica-portugal-february-2016
#20
Miguel Cueva
The third meeting organised by the European Federation of Biotechnology (EFB) on advances in Applied Synthetic Biotechnology in Europe (ASBE) was held in Costa da Caparica, Portugal, in February 2016. Abundant novel applications in synthetic biology were described in the six sessions of the meeting, which was divided into technology and tools for synthetic biology (I, II and III), bionanoscience, biosynthetic pathways and enzyme synthetic biology, and metabolic engineering and chemical manufacturing. The meeting presented numerous methods for the development of novel synthetic strains, synthetic biological tools and synthetic biology applications...
March 25, 2017: New Biotechnology
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