journal
MENU ▼
Read by QxMD icon Read
search

BMB Reports

journal
https://www.readbyqxmd.com/read/27931518/vitamin-c-enhances-the-expression-of-il17-in-a-jmjd2-dependent-manner
#1
Mi Hye Song, Varun Sasidharan Nair, Kwon Ik Oh
Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in CD4+Foxp3+ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes...
December 9, 2016: BMB Reports
https://www.readbyqxmd.com/read/27931517/glut1-promotes-cell-proliferation-migration-and-invasion-by-regulating-epidermal-growth-factor-receptor-and-integrin-signaling-in-triple-negative-breast-cancer-cells
#2
Sunhwa Oh, Hyungjoo Kim, KeeSoo Nam, Incheol Shin
Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/MAPK signaling pathway and integrin β1/Src/FAK signaling pathways...
December 9, 2016: BMB Reports
https://www.readbyqxmd.com/read/27916026/role-of-cyclic-amp-in-the-eye-of-glaucoma
#3
Myoung Sup Shim, Keun-Young Kim, Won-Kyu Ju
Glaucoma is characterized by a slow and progressive degeneration of optic nerve, including retinal ganglion cell (RGC) axons in the optic nerve head (ONH), leading to visual impairment. Despite the high prevalence, the biological basis of glaucoma pathogenesis still is not yet fully understood, and the factors contributing to its progression are currently not well characterized. Intraocular pressure (IOP) is the only modifiable risk factor, and reduction of IOP is the standard treatment for glaucoma. However, lowering IOP itself is not always effective for preserving visual function in patients with primary open-angle glaucoma...
December 5, 2016: BMB Reports
https://www.readbyqxmd.com/read/27916025/the-role-of-the-extracellular-biophysical-cues-in-modulating-hippo-yap-pathway
#4
Jung-Soon Mo
The Hippo signaling pathway plays an essential role in adult tissue homeostasis and organ size control. In Drosophila and vertebrates, it consists of a highly conserved kinase cascade, which involve MST and Lats that negatively regulate the activity of downstream transcription coactivators, YAP and TAZ. Through its interaction with TEADs and other transcription factors, they mediate both proliferative and antiapoptotic gene expression and thus regulate tissue repair and regeneration. Dysregulation or mutation of the Hippo pathway is linked to tumorigenesis and cancer development...
December 5, 2016: BMB Reports
https://www.readbyqxmd.com/read/27916024/neuron-specific-expression-of-p48-ebp1-during-murine-brain-development-and-its-contribution-to-cns-axon-regeneration
#5
Hyo Rim Ko, Inwoo Hwang, So Yoon Chang, Yun Sil Kim, Won Soon Park, Jee-Yin Ahn
P48 Ebp1 is expressed in rapidly proliferating cells such as cancer cells and accelerates cell growth and survival. However, its expression pattern and role in central nervous system development have not been studied. Here, we demonstrated the spatiotemporal expression pattern of p48 Ebp1 during embryonic development and the postnatal period. During embryonic development, p48 Ebp1 was highly expressed in the brain. Expression gradually decreased after birth but was still more abundant than p42 expression after birth...
December 5, 2016: BMB Reports
https://www.readbyqxmd.com/read/27881216/interacting-network-of-hippo-wnt-%C3%AE-catenin-and-notch-signaling-represses-liver-tumor-formation
#6
Wantae Kim, Sanjoy Kumar Khan, Yingzi Yang
Acquiring a selective growth advantage by breaking the proliferation barrier established by gatekeeper genes is a centrally important event in tumor formation. Removal of mammalian Hippo kinase Mst1 and Mst2 in hepatocytes leads to very rapid hepatocellular carcinoma (HCC) formation, indicating that the Hippo signaling pathway is a critical gatekeeper in hepatocytes that restrains abnormal growth. By rigorous genetic approaches, we have identified an interacting network of the Hippo, Wnt/β-catenin and Notch signaling pathways that controls organ size and HCC development...
November 24, 2016: BMB Reports
https://www.readbyqxmd.com/read/27881215/birth-of-an-asian-cool-reference-genome-ak1
#7
Changhoon Kim
The human reference genome, maintained by Genome Reference Consortium, is conceivably the most complete genome assembly ever since its first construction. It has been improved continually by incorporating corrections made to the previous assemblies thanks to various technological advances. Based on this reference genome, many population sequencing projects are ongoing to better represent human diversity, increasing hope for medical usage of genomic information, with recent maturation of the high-throughput sequencing technologies...
November 24, 2016: BMB Reports
https://www.readbyqxmd.com/read/27881214/zinc-ii-ion-promotes-anti-inflammatory-effects-of-rhsod3-by-increasing-cellular-association
#8
Younghwa Kim, Yoon-Jae Jeon, Kang Ryu, Tae-Yoon Kim
Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong biomedicine candidate. SOD3 accomplished anti-oxidant function without cell penetration and inhibited inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we have found that Zn2+ promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via heparin binding domain (HBD). We showed an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 into the nucleus, which is promoted by the presence of Zn2+...
November 24, 2016: BMB Reports
https://www.readbyqxmd.com/read/27866512/age-related-epigenetic-regulation-in-the-brain-and-its-role-in-neuronal-diseases
#9
Jeongsil Kim-Ha, Young-Joon Kim
Accumulating evidence indicates many brain functions are mediated by epigenetic regulation of neural genes, and their dysregulations result in neuronal disorders. Experiences such as learning and recall, and physical exercise induce neuronal activation through epigenetic modifications and by changing noncoding RNA profiles. Animal models, brain samples from patients, and the development of diverse analytical methods have broadened our understanding of epigenetic regulation in the brain. Diverse and specific epigenetic changes are being suggested to correlate with neuronal development, learning and memory, aging and age-related neuronal diseases...
November 18, 2016: BMB Reports
https://www.readbyqxmd.com/read/27866511/impaired-phagocytosis-of-apoptotic-cells-causes-accumulation-of-bone-marrow-derived-macrophages-in-aged-mice
#10
Ok-Hee Kim, Hyojung Kim, Jinku Kang, Dongki Yang, Yu-Hoi Kang, Dae Ho Lee, Gi Jeong Cheon, Sang Chul Park, Byung-Chul Oh
Accumulation of tissue macrophages is a significant characteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10...
November 18, 2016: BMB Reports
https://www.readbyqxmd.com/read/27802842/defective-anks1a-disrupts-the-export-of-receptor-tyrosine-kinases-from-the-endoplasmic-reticulum
#11
Soochul Park
EphA2 has been implicated in amplifying ErbB2 tumorigenic signaling. One protein that interacts with EphA2 is Anks1a PTB adaptor. However, the precise role of Anks1a in EphA2-mediated tumorigenesis is unclear. We demonstrated that Anks1a localizes to the ER upon phosphorylation and that the ankyrin repeats and PTB of Anks1a bind to EphA2 and Sec23, respectively. Thus, Anks1a facilitates the selective packaging of EphA2 into COPII vesicles. Additionally, Anks1a knock-out mice, a phenocopy of EphA2 knock-out mice, exhibited markedly reduced ErbB2-induced breast tumorigenesis...
November 2, 2016: BMB Reports
https://www.readbyqxmd.com/read/27802841/misexpression-of-attx12-encoding-a-toll-interleukin-1-receptor-domain-induces-growth-defects-and-expression-of-defense-related-genes-partially-independently-of-eds1-in-arabidopsis
#12
Sang-Kee Song
To identify functions of uncharacterized genes in Arabidopsis, tissue-specific GAL4/UAS activation tags were introduced into the Q2610 enhancer trap line, highly active in post-embryonic roots, and a dominant mutant exhibiting stunted growth named defective root development 1-D (drd1-D) was isolated. The T-DNA tag was located within the promoter region of AtTX12, which is predicted to encode a truncated nucleotide-binding leucine-rich repeat (NLR) protein, containing a Toll/interleukin-1 receptor (TIR) domain...
November 2, 2016: BMB Reports
https://www.readbyqxmd.com/read/27802840/mir-374-promotes-myocardial-hypertrophy-by-negatively-regulating-vascular-endothelial-growth-factor-receptor-1-signaling
#13
Jong Sub Lee, Lee Song, Jei Hyoung Park, Jin Ock Kim, Chunghee Cho, Do Han Kim
Vascular endothelial growth factor (VEGF) is an essential cytokine that has functions in the formation of new blood vessels and regression of cardiac hypertrophy. VEGF/VEGF-receptor-1(VEGFR1) signaling plays a key role in the regression of cardiac hypertrophy, whereas VEGF/VEGFR2 signaling leads to cardiac hypertrophy. In this study, we identified the prohypertrophic role of miR-374 using neonatal rat ventricular myocytes (NRVMs). Our results showed that overexpression of miR-374 activated G protein-coupled receptor-mediated prohypertrophic pathways by the inhibition of VEGFR1-dependent regression pathways...
November 2, 2016: BMB Reports
https://www.readbyqxmd.com/read/27756446/diversification-of-molecular-clockwork-for-tissue-specific-function-insight-from-novel-drosophila-clock-mutant-homologous-to-mouse-clock-allele
#14
Eunjoo Cho, Euna Lee, Eun Young Kim
The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of day. Transcriptional/translational feedback loop (TTFL) is the basic feature of eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression...
October 19, 2016: BMB Reports
https://www.readbyqxmd.com/read/27756445/effect-of-task-specific-training-on-eph-ephrin-expression-after-stroke
#15
Dong-Hee Choi, Jin-Hee Ahn, In-Ae Choi, Ji-Hye Kim, Bo-Ram Kim, Jongmin Lee
Recent evidence indicates that the ephrin receptors and ephrin ligands (Eph/ephrin) expression modulates axonal reorganization and synaptic plasticity in stroke recovery. To investigate the effect of task-specific training (TST) on Eph/ephrin expression in the corticospinal tract (CST) after stroke, we compared Eph/ephrin expression in the peri-infarct cortex, pyramid, and spinal cord of the photothrombotic stroke rats treated with or without TST. The TST treatment showed significantly better recovery in the behavioral tests...
October 19, 2016: BMB Reports
https://www.readbyqxmd.com/read/27756444/protective-effect-of-3-naphthalen-2-yl-propoxy-methyl-azetidine-hydrochloride-on-hypoxia-induced-toxicity-by-suppressing-microglial-activation-in-bv-2-cells
#16
Jiae Kim, Su-Min Kim, Jung-Min Na, Hoh-Gyu Hahn, Sung-Woo Cho, Seung-Ju Yang
We recently reported the anti-inflammatory effects of 3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride (KHG26792) on the ATP-induced activation of the NFAT and MAPK pathways through the P2X7 receptor in microglia. To further investigate the underlying mechanism of KHG26792, we studied its protective effects on hypoxia-induced toxicity in microglia. The administration of KHG26792 significantly reduced the hypoxia-induced expression and activity of caspase-3 in BV-2 microglial cells. KHG26792 also reduced hypoxia-induced inducible nitric oxide synthase protein expression, which correlated with reduced nitric oxide accumulation...
October 19, 2016: BMB Reports
https://www.readbyqxmd.com/read/27756443/human-adipose-derived-mesenchymal-stem-cell-spheroids-improve-recovery-in-a-mouse-model-of-elastase-induced-emphysema
#17
RyeonJin Cho, You-Sun Kim, Ji-Young Kim, Yeon-Mok Oh
BACKGROUND/PURPOSE: Emphysema, a pathologic component of chronic obstructive pulmonary disease, causes irreversible destruction. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. METHODS: We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in elastase-induced emphysema mice. ASCs were administered via an intrapleural route. RESULTS: Mice injected with spheroid ASCs showed improved regeneration of lung tissue, increased expression of growth factors such as FGF2 and HGF, and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs...
October 19, 2016: BMB Reports
https://www.readbyqxmd.com/read/27733233/extracellular-vesicles-as-novel-carrier-for-therapeutic-molecules
#18
Nambin Yim, Chulhee Choi
Extracellular vesicles (EVs) are natural carriers of biomolecules that play central roles in cell-to-cell communications. Based on this, there have been many attempts to use EVs as therapeutic drug carriers. Various macromolecules, from chemical reagents to nucleic acids, were successfully loaded into EVs; however, loading of proteins with high molecular weight has been huddled with several problems. Purification of recombinant proteins is expensive and time consuming, and easily results in modification of proteins by physical or chemical forces...
October 13, 2016: BMB Reports
https://www.readbyqxmd.com/read/27733232/crystallographic-snapshots-of-active-site-metal-shift-in-e-coli-fructose-1-6-bisphosphate-aldolase
#19
Huyen-Thi Tran, Seon-Hwa Lee, Thien-Hoang Ho, Seung-Hye Hong, Kim-Hung Huynh, Yeh-Jin Ahn, Deok-Kun Oh, Lin-Woo Kang
The fructose 1,6-bisphosphate aldolase (FBA) is important for both glycolysis and gluconeogenesis in life. Class II (zinc dependent) FBA is an attractive target for the development of antibiotics against protozoa, bacteria, and fungi and also widely used to produce various high-value stereoisomers in the chemical and pharmaceutical industry. In this study, crystal structures of class II Escherichia coli FBA (EcFBA) were determined from four different crystals with resolutions between 1.8 Å and 2.0 Å. Native EcFBA structures showed two separate sites of Zn1 (interior position) and Zn2 (active site surface position) for Zn2+ ion...
October 13, 2016: BMB Reports
https://www.readbyqxmd.com/read/27697112/polymer-brush-the-promising-grafting-approach-to-scaffolds-for-tissue-engineering
#20
Woonjung Kim, Jongjin Jung
Polymer brushes are the soft material units tethered covalently on the surface of scaffolds, which induce the functional and structural modification of a substrate's properties. Due to its facile fabrication, usability of various polymers, ECM (extracellular matrix)-like structural features, and in vivo stability, this surface coating approach has attracted special attentions in the fields of stem cell biology, tissue engineering, and regenerative medicine. Here, we summarized polymer brush-based grafting approaches comparing SAM (self-assembled monolayer)-based coating method in addition to physico-chemical characterization techniques of surfaces such as wettability, stiffness/elasticity, roughness, and chemical composition mainly affecting cell adhesion, differentiation, and proliferation...
September 29, 2016: BMB Reports
journal
journal
41871
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"