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BMB Reports

Banzhan Ruan, Chenxi Wang, Ang Chen, Jianqing Liang, Zubiao Niu, You Zheng, Jie Fan, Lihua Gao, Hongyan Huang, Xiaoning Wang, Qiang Sun
Homotypic cell-in-cell (CIC) structures forming between cancer cells were proposed to promote tumor evolution via entosis, a nonapoptotic cell death process. However, the mechanisms underlying their formation remained poorly understood. We performed a microarray analysis to identify genes associated with homotypic CIC formation. Cancer cells differing in their ability to form homotypic CIC structures were selected for the study. Association analysis identified 73 probe sets for 62 candidate genes potentially involved in CIC formation...
July 19, 2018: BMB Reports
Da Hyun Lee, Buhyun Lee, Jeong Su Park, Yu Seol Lee, Jin Hee Kim, Yejin Cho, Yoonjung Jo, Hyun-Seok Kim, Yong-Ho Lee, Ki Taek Nam, Soo Han Bae
Acetaminophen (APAP) overdose can cause hepatotoxicity by inducing mitochondrial damage and subsequent necrosis in hepatocytes. Sirtuin2 (Sirt2) is an NAD+-dependent deacetylase that regulates several biological processes, including hepatic gluconeogenesis, as well as inflammatory pathways. We show that APAP decreases the expression of Sirt2. Moreover, the ablation of Sirtuin2 attenuates APAP-induced liver injuries, such as oxidative stress and mitochondrial damage in hepatocytes. We found that Sirt2 deficiency alleviates the APAP -mediated endoplasmic reticulum stress and phosphorylation of the p70 ribosomal S6 kinase 1 (S6K1)...
July 19, 2018: BMB Reports
Hyung Sik Kim, Min Young Park, Sung Kyun Lee, Joon Seong Park, Ha Young Lee, Yoe-Sik Bae
Emergency granulopoiesis is a very important strategy to supply efficient neutrophil number in response to infection. However, molecular mechanism involved in this process remains unclear. Here, we found that administration of WKYMVm, an immune modulating peptide, to septic mice strongly increased neutrophil number through augmented emergency granulopoiesis. WKYMVm-induced emergency granulopoiesis was blocked not only by a formyl peptide receptor 2 (FPR2) antagonist (WRW4), but also by FPR2 deficiency. As progenitors of neutrophils, Lin-c-kit+Sca-1- cells expressed FPR2...
July 19, 2018: BMB Reports
Mulan Wei, Xujie Liu, Chunyu Cao, Jianlin Yang, Yafeng Lv, Jiaojiao Huang, Yanlin Wang, Ye Qin
Recent studies showed that the PD-1/PD-L1 checkpoint blockade is a dramatic therapy for melanoma by enhancing antitumor immune activity. Currently, major strategies for the PD-1/PD-L1 blockade have mainly focused on the use of antibodies and compounds. Seeking an alternative approach, others employ endogenous proteins as blocking agents. The extracellular domain of PD-1 (ePD1) includes the binding site with PD-L1. Accordingly, we constructed a PD-1-based recombinantly tailored fusion protein (dFv-ePD1) that consists of bivalent variable fragments (dFv) of an MMP-2/9-targeted antibody and ePD1...
July 19, 2018: BMB Reports
Yuree Lee, June M Kwak
Plants are unable to relocate themselves to a more favorable location and thus have to deal with developmental programs and environmental cues wherever they happen to be. It is yet largely unknown as to how plant cells coordinate cellular activities and architectures to accomplish developmental processes and responses to environmental change. By identifying and establishing a new cellular model system, we have recently uncovered that two neighboring cell types in abscission zone (AZ) of Arabidopsis flowers coordinate physiological states and elaborate differential cellular architectures to precisely separate organs...
July 3, 2018: BMB Reports
Seung-Ju Yang, Ji Woong Yang, Jung-Min Na, Ji Sun Ha, Soo Young Choi, Sung-Woo Cho
Parkinson's disease (PD) is a common chronic neurodegenerative disease mainly caused by the death of dopaminergic neurons. However, no complete pharmacotherapeutic approaches are currently available for PD therapies. 1-methyl-4-phenylpyridinium (MPP+)-induced SH-SY5Y neurotoxicity has been broadly utilized to create cellular models and study the mechanisms and critical aspects of PD. In the present study, we examined the role of a novel azetidine derivative, 3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride (KHG26792), against MPP+-induced neurotoxicity in SH-SY5Y cells...
July 3, 2018: BMB Reports
Ran Kim, Seokyeon Lee, Jihyun Lee, Minji Kim, Won Jung Kim, Hee Won Lee, Min Young Lee, Jongmin Kim, Woochul Chang
Exosomes are small membranous vesicles which contain abundant RNA molecules, and are transferred from releasing cells to uptaking cells. MicroRNA (miRNA) is one of the transferred molecules affecting the adopted cells, including glioma cells. We hypothesized that mesenchymal stem cells (MSCs) can secrete exosomes loading miRNA and have important effects on the progress of gliomas. To determine these effects by treating exosomal miRNA in culture media of miRNA mimic transfected MSCs, we assessed the in vitro cell proliferation and invasion capabilities, and the expression level of relative proteins associated with cell apoptosis, growth and migration...
July 3, 2018: BMB Reports
Mi Young Byun, Li Hua Cui, Hyoungseok Lee, Woo Taek Kim
Telomeres are specialized nucleoprotein complexes that function to protect eukaryotic chromosomes from recombination and erosion. Several telomere binding proteins (TBPs) have been characterized in higher plants, but their detailed in vivo functions at the plant level are largely unknown. In this study, we identified and characterized OsTRFL1 (Oryza sativa Telomere Repeat-binding Factor Like 1) in rice, a monocot model crop. Although OsTRFL1 did not directly bind to telomere repeats (TTTAGGG)4 in vitro, it was associated with telomeric sequences in planta...
June 25, 2018: BMB Reports
Hyeon Ji Yeo, Eun Ji Yeo, Min Jea Shin, Yeon Joo Choi, Chi Hern Lee, Hyeok Yil Kwon, Dae Won Kim, Won Sik Eum, Soo Young Choi
A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic β-cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F β-cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic β-cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion...
June 25, 2018: BMB Reports
Jong-Gil Park, Se-Jin Jeong, Jinha Yu, Gyudong Kim, Lak Shin Jeong, Goo Taeg Oh
Cardiovascular diseases arising from atherosclerosis are the leading causes of mortality and morbidity worldwide. Lipid-lowering agents have been developed in order to treat hypercholesterolemia, a major risk factor for atherosclerosis. However, the prevalence of cardiovascular diseases is increasing, indicating a need to identify novel therapeutic targets and develop new treatment agents. Adenosine receptors (ARs) are emerging as therapeutic targets in asthma, rheumatoid arthritis, cancer, ischemia, and inflammatory diseases...
June 25, 2018: BMB Reports
Jeesoo Chae, Weon Seo Park, Min Jung Kim, Se Song Jang, Dongwan Hong, Junsun Ryu, Chang Hwan Ryu, Ji-Hyun Kim, Moon-Kyung Choi, Kwan Ho Cho, Sung Ho Moon, Tak Yun, Jong-Il Kim, Yuh-Seog Jung
Secondary prevention via earlier detection would afford the greatest chance for a cure in premalignant lesions. We investigated the exomic profiles of non-malignant and malignant changes in head and neck squamous cell carcinoma (HNSCC) and the genomic blueprint of human papillomavirus (HPV)-driven carcinogenesis in oropharyngeal squamous cell carcinoma (OPSCC). Whole-exome (WES) and whole-genome (WGS) sequencing were performed on peripheral blood and adjacent non-tumor and tumor specimens obtained from eight Korean HNSCC patients from 2013 to 2015...
June 25, 2018: BMB Reports
Kyung Taek Kang, Yang Woo Kwon, Dae Kyoung Kim, Su In Lee, Ki-Hyung Kim, Dong-Soo Suh, Jae Ho Kim
Ovarian cancer is the most fatal gynecological malignancy in women and identification of new therapeutic targets is essential for the continued development of therapy for ovarian cancer. TRRAP (transformation/transcription domain-associated protein) is an adaptor protein and a component of histone acetyltransferase complex. The present study was undertaken to investigate the roles played by TRRAP in the proliferation and tumorigenicity of ovarian cancer stem cells. TRRAP expression was found to be up-regulated in the sphere cultures of A2780 ovarian cancer cells...
June 25, 2018: BMB Reports
Dong Hyun Jo, Jeong Hun Kim
Genome engineering with clustered regularly interspaced short palindromic repeats (CRISPR) system can be sued as a tool to correct pathological mutations or modulate gene expression levels associated with pathogenesis of human diseases. Owing to well-established local administration methods including intravitreal and subretinal injection, it is relatively easy to administer therapeutic genome engineering machinery to ocular tissues for treating retinal diseases. In this context, we have investigated the potential of in vivo genome engineering as a therapeutic approach in the form of ribonucleoprotein or CRISPR packaged in viral vectors...
June 20, 2018: BMB Reports
Su Hyun Jin, Hyunsoo Kim, Dong Ryun Gu, Keun Ha Park, Young Rae Lee, Yongwon Choi, Seoung Hoon Lee
Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of NF-κB ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner...
June 20, 2018: BMB Reports
Gaurav Agrahari, Shyam Kishor Sah, Tae-Yoon Kim
Therapeutic applications of mesenchymal stem cells (MSCs) are limited due to their early death within the first few days of transplantation. Therefore, to improve the efficacy of cell-based therapies, it is necessary to manipulate MSCs so that they can resist various stresses imposed by the microenvironment. Moreover, the role of superoxide dismutase 3 (SOD3) in regulating such survival under different stress conditions remain elusive. In this study, we overexpressed SOD3 in MSCs (SOD3-MSCs) and evaluated its effect under serum starvation conditions...
June 20, 2018: BMB Reports
Soo-Min Kim, Kyung-A Hwang, Kyung-Chul Choi
Reactive oxygen species (ROS) are major sources of cellular oxidative stress. Specifically, cancer cells harbor genetic alterations that promote a continuous and elevated production of ROS. While such oxidative stress conditions could be harmful to normal cells, they facilitate cancer cell growth in multiple ways by causing DNA damage and genomic instability, and ultimately, by reprogramming cancer cell metabolism. This review provides up to date findings regarding the role of ROS generation induced by diverse biological molecules and chemicals in representative women's' cancer...
June 20, 2018: BMB Reports
Chung-Hyun Cho, Keon Jin Lee, Eun Hui Lee
Skeletal muscle contracts or relaxes to maintain the body position and locomotion. For the contraction and relaxation of skeletal muscle, Ca2+ in the cytosol of skeletal muscle fibers acts as a switch to turn on and off a series of contractile proteins. The cytosolic Ca2+ level in skeletal muscle fibers is governed mainly by movement of Ca2+ between the cytosol and the sarcoplasmic reticulum (SR). Store-operated Ca2+ entry (SOCE), a Ca2+ entryway from the extracellular space to the cytosol, has gained a significant amount of attention from muscle physiologists...
June 14, 2018: BMB Reports
JaeIn Park, Jae-Hyun Jang, Geun-Soo Park, Yunro Chung, Hye Jin You, Jae-Hong Kim
Triple-negative breast cancer (TNBC) is considered to be a notorious type of cancer due to its aggressive metastatic potential and poor prognosis. Recent evidence suggests that BLT2, a low-affinity LTB4 receptor is critically associated with the phenotypes of TNBC cells, including invasion, metastasis, and survival. Furthermore, in a group of 545 breast cancer patients with metastasis, we observed that the high-BLT2 subgroup had a lower disease-free-survival rate than the low-BLT2 subgroup. Thus, we theorized that anti-BLT2 strategies could facilitate the development of new therapies used for TNBC...
June 14, 2018: BMB Reports
Miri Choi, Jiyeon Baek, Sang-Bae Han, Sungchan Cho
Protein-Protein Interactions (PPIs) play essential roles in diverse biological processes and their misregulations are associated with a wide range of diseases. Especially, the growing attention to PPIs as a new class of therapeutic target is increasing the need for an efficient method of cell-based PPI analysis. Thus, we newly developed a robust PPI assay (SeePPI) based on the co-translocation of interacting proteins to the discrete subcellular compartment 'processing body' (p-body) inside living cells, enabling a facile analysis of PPI by the enriched fluorescent signal...
June 14, 2018: BMB Reports
Yongjie Xu, Rui Li, Kaili Zhang, Wei Wu, Suying Wang, Pengpeng Zhang, Haixia Xu
HnRNPK is a multifunctional protein that participates in chromatin remodeling, transcrip-tion, RNA splicing, mRNA stability and translation. Here, we uncovered the function of hnRNPK in regulating the proliferation and differentiation of myoblasts. hnRNPK was mutated in the C2C12 myoblast cell line using the CRISPR/Cas9 system. A decreased proliferation rate was observed in hnRNPK-mutated cells, suggesting an impaired prolif-eration phenotype. Furthermore, increased G2/M phase, decreased S phase and increased sub-G1 phase cells were detected in the hnRNPK-mutated cell lines...
June 14, 2018: BMB Reports
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