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Systems Biology in Reproductive Medicine

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https://www.readbyqxmd.com/read/28795845/analysis-of-embryo-intactness-and-developmental-potential-following-slow-freezing-and-vitrification
#1
Lan Yu, Chanwei Jia, Yonglian Lan, Rui Song, Liying Zhou, Ying Li, Yu Liang, Shuyu Wang
The aim of this study was to identify the parameters that are related to intactness and developmental potential of a day 3 embryo after warming to improve the selection criteria used to cryopreserve and transfer embryos. We also sought to compare slow freezing and vitrification methods of cryopreservation and to evaluate the viability of non-intact embryos. Embryos warmed following slow freezing (n=220) or vitrification (n=522) were divided into 3 groups according to the proportion of surviving blastomeres (I<50%; II=50-99%; and III=100%)...
August 10, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28787189/sperm-na-k-atpase-%C3%AE-4-and-plasma-membrane-ca-2-atpase-pmca-4-regulation-in-asthenozoospermia
#2
Silvia W Lestari, Dessy Noor Miati, P Seoharso, R Sugiyanto, Dwi A Pujianto
Asthenozoospermia, which is characterized by reduced motility, is one of the etiologies of male infertility. Its biochemical and functional consequences include altered ATPase activity. This study investigated the activities of Na(+), K(+)-ATPase and Ca(2+)-ATPase and the expression of Na(+), K(+)-ATPase α4 and PMCA4 isoforms in human sperm of asthenozoospermic infertile men. Nineteen samples from asthenozoospermic infertile couples were examined in this study. Computerized-assisted semen analysis (CASA) was performed, and the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate...
August 8, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28771045/expression-patterns-and-changes-of-the-lcn2-gene-in-the-testes-of-induced-cryptorchidism-and-busulfan-treated-mice
#3
Zhenlong Kang, Na Qiao, Zhigang Tan, Zhaoxin Tang, Ying Li
Lipocalin-2 (LCN2) was known to play various roles in different type cells; however, little was known about the effect of LCN2 in male fertility. In this study, we aimed to explore the expression pattern of LCN2 with increasing age in mice, and to obtain insight into the role of LCN2 in mice testes by induced cryptorchidism and busulfan-treated infertility. In situ hybridization showed that LCN2 was localized primarily in Leydig cells, but was absent in Sertoli and germ cells. Its expression in testes exhibited an age-related increase from day 1 to 8 months, then reduced by the twelth month...
August 3, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28771041/obstructive-azoospermia-caused-by-low-ligation-of-varicocele-a-case-report
#4
Jun Hagiuda, Hiromichi Ishikawa, Masayuki Hagiwara, Hidaka Kono, Ken Nakagawa, Mototsugu Oya
Varicocele is commonly observed in male partners of infertile couples. The surgical ligation of varicoceles under microscopy can be safely performed by a skilled andrologist with most patients subsequently experiencing an improvement in semen quality. This is the first case in which obstructive azoospermia occurred after high inguinal varicocelectomy. The vas deferens was disrupted near the internal inguinal ring by fibrous tissue. A vasovasostomy was performed and semen parameters subsequently recovered.
August 3, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28696790/competitive-inhibition-of-amino-acid-transport-in-human-preovulatory-ovarian-follicles
#5
Maciej Jóźwik, Marcin Jóźwik, Anna Justyna Milewska, Frederick C Battaglia, Michał Jóźwik
To date we have yet to examine whether amino acid (AA) transport in human ovarian follicles is affected by competitive inhibition. In contrast, transplacental transfer of AAs in late-gestation sheep is characterized by reciprocal competition. This phenomenon has been described by algebraic equations of umbilical uptake of AAs based on maternal arterial concentrations. In the present translational study at a university teaching hospital, we verified whether these equations apply to the transport of AAs from blood to follicular fluid (FF) in human preovulatory follicles...
July 11, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28632990/shortening-gametes-co-incubation-time-improves-live-birth-rate-for-couples-with-a-history-of-fragmented-embryos
#6
Anne Le Bras, Laetitia Hesters, Vanessa Gallot, Cathie Tallet, Gerard Tachdjian, Nelly Frydman
Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study was designed to evaluate the efficiency of SGCO in a poor prognosis population with a history of fragmented embryos defined by the presence of at least 50% of the embryos with more than 25% of cytoplasmic fragments...
June 20, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28609124/the-impact-of-laparoscopic-surgery-of-peritoneal-endometriosis-and-endometrioma-on-the-outcome-of-icsi-cycles
#7
Ismail Guler, Ahmet Erdem, Yuksel Oguz, Funda Cevher, Mehmet Firat Mutlu, Nuray Bozkurt, Mesut Oktem, Mehmet Erdem
Our objective was to assess the role of laparoscopic removal of ovarian endometriomas and ablation of peritoneal endometriosis on the outcome of intracytoplasmic sperm injection (ICSI) - Embryo Transfer cycles by comparing with the results of patients with untreated endometriomas and tubal factor without underlying endometriosis confirmed by laparoscopy. For this purpose, between 2002 and 2015, outcomes of 257 ICSI cycles of 150 patients, including 91 cycles of 48 patients in minimal endometriosis, 57 cycles of 25 patients in endometrioma removal, 65 cycles of 53 patients in non-operated endometrioma, and 44 cycles of 24 patients in tubal factor groups were retrospectively analyzed...
June 13, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28632452/the-effect-of-g-csf-on-infertile-women-undergoing-ivf-treatment-a-meta-analysis
#8
Jie Li, Sien Mo, Yang Chen
Evidence for the effect of granulocyte colony stimulating factor (G-CSF) on infertile women undergoing in vitro fertilization (IVF) remains inconsistent. This study aimed to evaluate the effectiveness of G-CSF on infertile women undergoing IVF. PubMed and EMBASE databases were searched before August 2016. Comparing the transvaginal perfusion of G-CSF and placebo or no treatment, the available studies were considered. The pooled risk ratio (RR) with 95% confidence intervals (CIs) was used in the analysis and six studies were included...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28574782/rna-based-ovarian-cancer-research-from-a-gene-to-systems-biomedicine-perspective
#9
Esra Gov, Medi Kori, Kazim Yalcin Arga
Ovarian cancer remains the leading cause of death from a gynecologic malignancy, and treatment of this disease is harder than any other type of female reproductive cancer. Improvements in the diagnosis and development of novel and effective treatment strategies for complex pathophysiologies, such as ovarian cancer, require a better understanding of disease emergence and mechanisms of progression through systems medicine approaches. RNA-level analyses generate new information that can help in understanding the mechanisms behind disease pathogenesis, to identify new biomarkers and therapeutic targets and in new drug discovery...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28485996/impact-of-hydrosalpinx-fluid-on-early-human-embryos
#10
Hongchu Bao, Qinglan Qu, Xin Huang, Meimei Wang, Xinrong Wang, Cuifang Hao
This study aimed to investigate the impact of hydrosalpinx fluid (HF) on early human embryonic development. A total of 33 patients who underwent laparoscopic surgery for hydrosalpinx were selected, and the HF specimens obtained from these patients were subjected to bacterial culture, Chlamydia antigen detection, biochemical analysis, and cytokine detection. Meanwhile, human embryos derived from three pronuclei (3PN) were cultured in various HF concentrations. There was no significant difference in the chemical components and physical characteristics between colorless and colored HF specimens, apart from the glucose concentration which was significantly higher in colorless HF...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28441042/aberrantly-expressed-long-noncoding-rnas-in-recurrent-implantation-failure-a-microarray-related-study
#11
Li-Juan Fan, Hong-Jing Han, Jing Guan, Xiao-Wei Zhang, Qing-Hua Cui, Huan Shen, Cheng Shi
Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs longer than 200 nucleotides. They were long regarded as transcription noise for their low expression and non-protein coding features. Recent published reports indicate that lncRNAs are involved in virtually every aspect of human biology. We aimed to profile the endometrial lncRNA expression pattern in women with recurrent implantation failure (RIF) and predict the function of the genes of the dysregulated lncRNA transcripts. Endometrial samples (24) were collected during window of implantation (14 RIF women and 10 women who conceived after embryo transfer)...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28388287/in-silico-analysis-of-non-synonymous-single-nucleotide-polymorphisms-in-human-dazl-gene-associated-with-male-infertility
#12
Mili Nailwal, Jenabhai B Chauhan
In living systems the most frequent type of genetic mutation is the single nucleotide polymorphism (SNP). Non-synonymous SNPs (nsSNPs) or missense mutations arise in coding regions of a particular gene. nsSNPs result in a single amino acid substitution which may have effects on the structure and/or function of proteins. Spermatogenesis is a complex process where haploid spermatozoa are formed. The deleted in azoospermia like (DAZL) gene has a relationship with male infertility and dysfunction of DAZL may decrease the sperm count which leads to oligozoospermia or azoospermia...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28345968/relationship-between-phospholipase-c-zeta-semen-parameters-and-chromatin-status
#13
Marziyeh Tavalaee, Abbas Kiani-Esfahani, Mohammad H Nasr-Esfahani
The need for additional tests to complement basic sperm analysis in clinics is well appreciated. In this regard, a number of tests such as sperm DNA integrity test as a tool in diagnosis and treatment of infertility are suggested. But recent studies have focused on main sperm factors involved in oocyte activation such as phospholipase C-zeta (PLCζ) that initiate intracellular Ca(2+) signaling and embryogenesis. Therefore, this study aimed to investigate the relationship between PLCζ, basic semen parameters, sperm DNA fragmentation (SDF), and protamine deficiency in men with normal (n=32) and abnormal (n=23) semen parameters...
August 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28394645/new-protocol-based-on-massive-parallel-sequencing-for-aneuploidy-screening-of-preimplantation-human-embryos
#14
Xavier Vendrell, Victoria Fernández-Pedrosa, Juan Carlos Triviño, Rosa Bautista-Llácer, Carmen Collado, Oscar Rodríguez, Elena García-Mengual, Empar Ferrer, Carmen Calatayud, Miguel Ruiz-Jorro
Novel next-generation sequencing procedures have rapidly emerged into the preimplantation genetic screening framework. This work presents the design and validation of a new low-coverage whole-genome sequencing assay for aneuploidy detection in single blastomeres and trophectodermal samples from preimplantation embryos. The validation ensures analytical sensitivity, specificity, robustness, precision, limit of detection, resolution, and reproducibility. Specific parameters to measure the performance are defined, and the results are compared with a standardized array-based method to stablish the concordance...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28345956/modulation-of-steroidogenesis-by-vitamin-d3-in-granulosa-cells-of-the-mouse-model-of-polycystic-ovarian-syndrome
#15
Shabnam Bakhshalizadeh, Fardin Amidi, Ashraf Alleyassin, Masoud Soleimani, Reza Shirazi, Maryam Shabani Nashtaei
Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder of women of reproductive age characterized by polycystic ovarian morphology, anovulation or oligomenorrhea, and hyperandrogenism. It is shown that disruption in the steroidogenesis pathway caused by excess androgen in PCOS is a critical element of abnormal folliculogenesis and failure in dominant follicle selection. Vitamin D plays an important role in the regulation of ovulatory dysfunction and can influence genes involved in steroidogenesis in granulosa cells...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28340305/the-clinical-application-of-ngs-based-snp-haplotyping-for-pgd-of-hb-h-disease
#16
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
This study investigated the usefulness of next-generation sequencing (NGS)-based single nucleotide polymorphism (SNP) haplotyping for preimplantation genetic diagnosis (PGD) of hemoglobin H (Hb H) disease. Multiple displacement amplification (MDA) was used for whole genome amplification (WGA) of biopsied trophectoderm (TE) cells. Gap-PCR and NGS-based SNP haplotyping was used to distinguish the two genotypes of -α(3.7)/αα and -(SEA)/αα for PGD of Hb H disease. One out of the ten blastocysts (B11) was successfully diagnosed as genotype -α(3...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28332864/effect-of-density-gradient-centrifugation-on-reactive-oxygen-species-in-human-semen
#17
Teppei Takeshima, Yasushi Yumura, Shinnosuke Kuroda, Takashi Kawahara, Hiroji Uemura, Akira Iwasaki
Density gradient centrifugation can separate motile sperm from immotile sperm and other cells for assisted reproduction, but may also remove antioxidants from seminal plasma, resulting in oxidative stress. Therefore, we investigated reactive oxygen species (ROS) concentrations and distribution in semen before and after density gradient centrifugation. We assessed semen volume, sperm concentration, sperm motility, and ROS levels before and after density gradient centrifugation (300 x g for 20 minutes) in 143 semen samples from 118 patients...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306344/live-birth-after-replacement-of-an-embryo-obtained-from-a-spontaneously-in-vitro-matured-metaphase-i-oocyte
#18
Paloma Piqueras, Miguel Gallardo, María Hebles, Juan Manuel Jiménez, Beatriz Migueles, Lorena Montero, Fernando Sánchez-Martín, Pascual Sánchez-Martín
This case report describes a live birth after the fresh replacement of an embryo obtained from a spontaneously in vitro matured oocyte. The patient was subjected to controlled ovarian stimulation for IVF treatment, obtaining two oocytes. One was found to be immature at the time of denudation, at metaphase-I. This immature oocyte was kept in culture overnight in standard conditions along with the second oocyte - which was mature but failed to fertilize - spontaneously achieving metaphase-II, and was subjected to ICSI...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306342/nmr-based-plasma-metabolomic-discrimination-for-male-fertility-assessment-of-rats-treated-with-eurycoma-longifolia-extracts
#19
Forough Ebrahimi, Baharudin Ibrahim, Chin-Hoe Teh, Vikneswaran Murugaiyah, Kit-Lam Chan
Male infertility is one of the leading causes of infertility which affects many couples worldwide. Semen analysis is a routine examination of male fertility status which is usually performed on semen samples obtained through masturbation that may be inconvenient to patients. Eurycoma longifolia (Tongkat Ali, TA), native to Malaysia, has been traditionally used as a remedy to boost male fertility. In our recent studies in rats, upon the administration of high-quassinoid content extracts of TA including TA water (TAW), quassinoid-rich TA (TAQR) extracts, and a low-quassinoid content extract including quassinoid-poor TA (TAQP) extract, sperm count (SC) increased in TAW- and TAQR-treated rats when compared to the TAQP-treated and control groups...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306341/implantation-potential-of-mosaic-embryos
#20
Belén Lledó, Ruth Morales, Jose Antonio Ortiz, Helena Blanca, Jorge Ten, Joaquín Llácer, Rafael Bernabeu
Chromosomal mosaicism is a relatively common finding in human IVF embryos. However, the association between mosaicism in trophoectoderm and inner mass cells, the mechanisms involved, and its effects on implantation are far from established. We retrospectively reanalyzed array-CGH results from 1,362 trophoectoderm biopsies. We detected chromosomal mosaicism in 183 blastocysts (13.4%). A decrease in the clinical pregnancy rate when we compared the cycles where only mosaic embryos were transferred (26.9%) vs. euploid embryos were transferred (40...
June 2017: Systems Biology in Reproductive Medicine
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