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Systems Biology in Reproductive Medicine

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https://www.readbyqxmd.com/read/28340305/the-clinical-application-of-ngs-based-snp-haplotyping-for-pgd-of-hb-h-disease
#1
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
This study investigated the usefulness of next-generation sequencing (NGS)-based single nucleotide polymorphism (SNP) haplotyping for preimplantation genetic diagnosis (PGD) of hemoglobin H (Hb H) disease. Multiple displacement amplification (MDA) was used for whole genome amplification (WGA) of biopsied trophectoderm (TE) cells. Gap-PCR and NGS-based SNP haplotyping was used to distinguish the two genotypes of -α(3.7)/αα and -(SEA)/αα for PGD of Hb H disease. One out of the ten blastocysts (B11) was successfully diagnosed as genotype -α(3...
March 24, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28332864/effect-of-density-gradient-centrifugation-on-reactive-oxygen-species-in-human-semen
#2
Teppei Takeshima, Yasushi Yumura, Shinnosuke Kuroda, Takashi Kawahara, Hiroji Uemura, Akira Iwasaki
Density gradient centrifugation can separate motile sperm from immotile sperm and other cells for assisted reproduction, but may also remove antioxidants from seminal plasma, resulting in oxidative stress. Therefore, we investigated reactive oxygen species (ROS) concentrations and distribution in semen before and after density gradient centrifugation. We assessed semen volume, sperm concentration, sperm motility, and ROS levels before and after density gradient centrifugation (300 x g for 20 minutes) in 143 semen samples from 118 patients...
March 23, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306344/live-birth-after-replacement-of-an-embryo-obtained-from-a-spontaneously-in-vitro-matured-metaphase-i-oocyte
#3
Paloma Piqueras, Miguel Gallardo, María Hebles, Juan Manuel Jiménez, Beatriz Migueles, Lorena Montero, Fernando Sánchez-Martín, Pascual Sánchez-Martín
This case report describes a live birth after the fresh replacement of an embryo obtained from a spontaneously in vitro matured oocyte. The patient was subjected to controlled ovarian stimulation for IVF treatment, obtaining two oocytes. One was found to be immature at the time of denudation, at metaphase-I. This immature oocyte was kept in culture overnight in standard conditions along with the second oocyte - which was mature but failed to fertilize - spontaneously achieving metaphase-II, and was subjected to ICSI...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306342/nmr-based-plasma-metabolomic-discrimination-for-male-fertility-assessment-of-rats-treated-with-eurycoma-longifolia-extracts
#4
Forough Ebrahimi, Baharudin Ibrahim, Chin-Hoe Teh, Vikneswaran Murugaiyah, Kit-Lam Chan
Male infertility is one of the leading causes of infertility which affects many couples worldwide. Semen analysis is a routine examination of male fertility status which is usually performed on semen samples obtained through masturbation that may be inconvenient to patients. Eurycoma longifolia (Tongkat Ali, TA), native to Malaysia, has been traditionally used as a remedy to boost male fertility. In our recent studies in rats, upon the administration of high-quassinoid content extracts of TA including TA water (TAW), quassinoid-rich TA (TAQR) extracts, and a low-quassinoid content extract including quassinoid-poor TA (TAQP) extract, sperm count (SC) increased in TAW- and TAQR-treated rats when compared to the TAQP-treated and control groups...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306341/implantation-potential-of-mosaic-embryos
#5
Belén Lledó, Ruth Morales, Jose Antonio Ortiz, Helena Blanca, Jorge Ten, Joaquín Llácer, Rafael Bernabeu
Chromosomal mosaicism is a relatively common finding in human IVF embryos. However, the association between mosaicism in trophoectoderm and inner mass cells, the mechanisms involved, and its effects on implantation are far from established. We retrospectively reanalyzed array-CGH results from 1,362 trophoectoderm biopsies. We detected chromosomal mosaicism in 183 blastocysts (13.4%). A decrease in the clinical pregnancy rate when we compared the cycles where only mosaic embryos were transferred (26.9%) vs. euploid embryos were transferred (40...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28306340/a-potential-functional-association-between-mutant-bmpr2-and-primary-ovarian-insufficiency
#6
Liliana Catherine Patiño, Daniel Silgado, Paul Laissue
Primary ovarian insufficiency (POI) affects ~1% of women in the general population. Despite numerous attempts at identifying POI genetic aetiology, coding mutations in only a few genes have been functionally related to POI pathogenesis. It has been suggested that mutant BMPR2 might contribute towards the phenotype. Several BMP15 (a BMPR2 ligand) coding mutations in human species have been related to POI pathogenesis. The BMPR2 p.Ser987Phe mutation, previously identified in a woman with POI, might therefore lead to cellular dysfunction contributing to the phenotype...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28277744/a-modified-vitrification-method-reduces-spindle-and-chromosome-abnormalities
#7
Minghui Liu, Wenhui Zhou, Dapeng Chu, Lei Fu, Wei Sha, Shan Liu, Yuan Li
Development of an effective system for oocyte-cryopreservation is of clinical relevance in reproductive medicine. However, oocyte-preservation is not as effective as embryo preservation. In this study, we used a 37°C pre-equilibrium temperature as part of a modified vitrification method for human oocyte cryopreservation. The effect of the new method on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated in in vitro-matured human oocytes. A total of 101 in vitro-matured oocytes were randomly assigned for vitrification at pre-equilibrium temperature of 37°C (37°C Group, n=50) or at room temperature (RT Group, 22-24°C, n=51)...
March 9, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28045561/age-related-changes-in-gene-expression-patterns-of-immature-and-aged-rat-primordial-follicles
#8
Vijayakumar Govindaraj, Harshini Krishnagiri, Payal Chakraborty, Madavan Vasudevan, A Jagannadha Rao
Women are born with millions of primordial follicles which gradually decrease with increasing age and this irreversible supply of follicles completely exhausts at menopause. The fertility capacity of women diminishes in parallel with aging. The mechanisms for reproductive aging are not fully understood. We have observed a decline in Brca1 mediated DNA repair in aging rat primordial follicles. To further understand the age-related molecular changes, we performed microarray gene expression analysis using total RNA extracted from immature (18 to 20 day old) and aged (400 to 450 day old) rat primordial follicles...
January 3, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301258/maldi-mass-spectrometry-reveals-that-cumulus-cells-modulate-the-lipid-profile-of-in-vitro-matured-bovine-oocytes
#9
Alessandra A Vireque, Alessandra Tata, Katia Roberta A Belaz, João Gabriel V Grázia, Fábio N Santos, Daniel R Arnold, Andrea C Basso, Marcos N Eberlin, Marcos Felipe Silva-de-Sá, Rui A Ferriani, Ana Carolina J Sá Rosa-E-Silva
The influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squares-discriminant analysis (PLS-DA)...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301257/gp91phox-nadph-oxidase-modulates-litter-size-by-regulating-mucin1-in-the-uterus-of-mice
#10
Keiichi Hiramoto, Yurika Yamate, Eisuke F Sato
Active oxygen derived from gp91phox is critical for gestation. However, no reports have evaluated the relationship between reactive oxygen species (ROS) and the number of births in a given pregnancy. In this study, we examined the influence of ROS produced by gp91phox activity on the number of births using C57BL/6j (control) and gp91phox-knockout (gp91phox(-/-)) mice. The number of births in gp91phox(-/-) mice was found to be lower than that in control mice. We observed sequential increases in gp91phox, ROS, nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3), caspase-1, and interleukin-18 (IL-18), followed by increased expression of mucin1 (MUC1), in control mice...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301256/sperm-epigenetics-in-the-study-of-male-fertility-offspring-health-and-potential-clinical-applications
#11
Timothy G Jenkins, Kenneth I Aston, Emma R James, Douglas T Carrell
The mammalian sperm contains a highly unique and specialized epigenetic landscape that offers a great degree of interesting research opportunities. One key discriminating feature of the mature sperm epigenome is that it, in theory, represents both remnant marks used throughout spermatogenesis to generate sperm cells competent to perform their function, but also marks that appear to be useful beyond fertilization. Key questions must be asked about the utility of these marks and the multiple purposes that may be served...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301255/the-cell-state-splitter-embryogenesis-explained-a-review-by-david-millergordon-natalie-k-and-gordon-richard-embryogenesis-explained-world-scientific-publishing-co-singapore-2016-784-pages-isbn-978-981-4350-48-8
#12
https://www.readbyqxmd.com/read/28301254/embryogenesis-explained-prolegomenon
#13
Natalie K Gordon
No abstract text is available yet for this article.
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28301253/efficacy-and-safety-of-varicocelectomies-a-meta-analysis
#14
Renbin Yuan, Hui Zhuo, Dehong Cao, Qiang Wei
This study reviewed the efficacy and safety of the three surgical approaches for varicocele (microsurgical, laparoscopic, and open varicocelectomy). A systematic review of the relevant randomized clinical trials was performed. Trials were identified from specialized trials register of the Cochrane UGDP Group, the Cochrane library, additional electronic searches (mainly MEDLINE, EMBSAE, SCI, CBM), and handsearching. Clinical trials comparing microsurgical, laparoscopic and open varicocelectomies were included...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28151020/clinical-outcome-of-cycles-with-oocyte-degeneration-after-intracytoplasmic-sperm-injection
#15
Lanlan Liu, Jiali Cai, Ping Li, Xiaoming Jiang, Jianzhi Ren
There are variant rates of oocyte degeneration after intracytoplasmic sperm injection (ICSI) among different patients. Oocyte degeneration after ICSI may reflect the cohort of oocyte quality and subsequent embryo development capacity and clinical outcome. This retrospective study analyzed 255 cycles with at least one degenerated oocyte after ICSI (degeneration group) and 243 cycles with no degenerated oocytes after ICSI (control group). Basic characteristics like female age, body mass index, duration of infertility, hormone (FSH, LH, E2) levels on day 3 of menses, and primary infertility patient rate were similar between the two groups (p > 0...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28103109/impaired-spermatogenesis-in-the-twitcher-mouse-a-morphological-evaluation-from-the-seminiferous-tubules-to-epididymal-transit
#16
Alice Luddi, Martina Gori, Laura Crifasi, Camilla Marrocco, Giuseppe Belmonte, Elvira Costantino-Ceccarini, Paola Piomboni
Spermatogenesis is a complex process of proliferation and differentiation during male germ cell development whereby undifferentiated spermatogonial germ cells evolve into maturing spermatozoa. In this developmental process the interactions between different cell types are finely regulated, hence any disruption in these relationships leads to male infertility. The twitcher mouse, the murine model of Krabbe disease, is characterized by deficiency of galactosylceramidase, an enzyme also involved in the metabolism of the galactosyl-alkyl-acyl-glycerol, the precursor of sulfogalactosyl-alkyl-acyl-glycerol, the most abundant glycolipid in spermatozoa...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28085499/a-sperm-specific-proteome-scale-metabolic-network-model-identifies-non-glycolytic-genes-for-energy-deficiency-in-asthenozoospermia
#17
Arvand Asghari, Sayed-Amir Marashi, Naser Ansari-Pour
About 15% of couples experience difficulty in conceiving a child, of which half of the cases are thought to be male-related. Asthenozoospermia, or low sperm motility, is one of the frequent types of male infertility. Although energy metabolism is suggested to be central to the etiology of asthenozoospermia, very few attempts have been made to identify its underlying metabolic pathways. Here, we reconstructed SpermNet, the first proteome-scale model of the sperm cell by using whole-proteome data and the mCADRE algorithm...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28139180/personalized-precision-reproductive-medicine-and-diagnostics
#18
Stephen A Krawetz
No abstract text is available yet for this article.
February 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28139179/corrigendum
#19
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28010131/predicting-the-clinical-outcome-of-icsi-by-sperm-head-vacuole-examination
#20
Khaled Pocate-Cheriet, Ilan Heilikman, Raphael Porcher, Virginie Barraud-Lange, Nathalie Sermondade, Charlene Herbemont, Jean Philippe Wolf, Christophe Sifer
To assess whether high magnification sperm head vacuole examination (SHVE) and/or standard sperm morphology assessment can predict ICSI outcomes in terms of fertilization, embryo quality, and delivery rates, a prospective observational bicentric study was conducted in two publicly funded assisted reproductive technology (ART) units in France between January and July of 2012. A total of 111 ICSI cycles for exclusively male infertility factors were included. A Spearman's correlation test was performed to validate SHVE reproducibility between the ART units...
December 23, 2016: Systems Biology in Reproductive Medicine
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