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Systems Biology in Reproductive Medicine

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https://www.readbyqxmd.com/read/29985652/comparison-between-paraffin-and-mineral-oil-covering-on-early-human-embryo-culture-a-prospective-randomized-study
#1
Soraya Labied, Caroline Jouan, Frédéric Wenders, Stéphanie Ravet, Olivier Gaspard, Fabienne Thonon, Virginie Gridelet, Laurie Henry, Sophie Perrier d'Hauterive, Michelle Nisolle
The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil®, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by  intracytoplasmic sperm injection (ICSI) were enrolled in our study...
July 9, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29975109/is-growth-hormone-administration-essential-for-in-vitro-fertilization-treatment-of-female-patients-with-growth-hormone-deficiency
#2
Dragoş Albu, Alice Albu
Available evidence suggests that the fertility of growth hormone deficient female patients could be decreased, although the responsible mechanisms are unknown. Taking into account the multiple effects of growth hormone on reproduction suggested by experimental and clinical studies in women without growth hormone deficiency, the growth hormone deficit by itself could contribute to infertility in these patients. However, the necessity of growth hormone administration and the profile of the infertile patients with growth hormone deficiency who would benefit from treatment are largely unknown...
July 5, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29965778/application-of-metabolomics-to-preeclampsia-diagnosis
#3
B Fatemeh Nobakht M Gh
Preeclampsia is a multifactorial disorder defined by hypertension and increased urinary protein excretion during pregnancy. It is a significant cause of maternal and neonatal deaths worldwide. Despite various research efforts to clarify pathogenies of preeclampsia and predict this disease before beginning of symptoms, the pathogenesis of preeclampsia is unclear. Early prediction and diagnosis of women at risk of preeclampsia has not markedly improved. Therefore, the objective of this study was to perform a review on metabolomic articles assessing predictive and diagnostic biomarkers of preeclampsia...
July 2, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29952660/successful-application-of-a-single-warming-protocol-for-embryos-cryopreserved-by-either-slow-freezing-or-vitrification-techniques
#4
Munevver Serdarogullari, Onder Coban, Fazilet Kubra Boynukalin, Ekrem Murat Bilgin, Necati Findikli, Mustafa Bahceci
The aim of this study was to evaluate the feasibility and efficiency of using a sucrose gradient-based warming protocol as a universal warming approach on human cleavage stage embryos. Between January 2013 and November 2014, a total of 118 warming cycles were performed on 705 embryos which had previously been cryopreserved/thawed by slow freezing protocols or cryopreserved by slow freezing and warmed by vitrification thaw solution. Clinical outcomes have been retrospectively analyzed depending on cryopreservation and warming techniques used, embryo viability, day of cryopreservation, clinical pregnancy, implantation, and live birth rate...
June 28, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29939100/identification-of-a-complex-population-of-chromatin-associated-proteins-in-the-european-sea-bass-dicentrarchus-labrax-sperm
#5
Ferran Barrachina, Dafni Anastasiadi, Meritxell Jodar, Judit Castillo, Josep Maria Estanyol, Francesc Piferrer, Rafael Oliva
A very common conception about the function of the spermatozoon is that its unique role is to transmit the paternal genome to the next generation. Most of the sperm genome is known to be condensed in many species by protamines, which are small and extremely positively charged proteins (50-70% arginine) with the functions of streamlining the sperm cell and protecting its DNA. However, more recently, it has been shown in mammals that 2-10% of its mature sperm chromatin is also associated to a complex population of histones and chromatin-associated proteins differentially distributed in the genome...
June 25, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29927665/maternal-reproductive-performance-and-fetal-development-of-the-wistar-audiogenic-rat-war-strain
#6
Eduardo H Umeoka, Matheus C Eiras, Iara G Viana, Vanessa S Giorgi, Aline Bueno, Débora C Damasceno, Norberto Garcia-Cairasco, Paula A Navarro
Wistar Audiogenic Rat (WAR) strain is an animal model for epilepsy studies, the chronic multifactorial disease that affects millions of people worldwide. The animals of this strain are genetically predisposed to sound-induced seizures, called audiogenic seizures, and have been used for many years in studies to understand the mechanisms involved in the epilepsies and their neuropsychiatric comorbidities, as well as the screening of potential anti-convulsant agents. Nevertheless, little is known about the reproductive characteristics of these animals...
June 21, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29916276/association-of-estrogen-progesterone-and-follicle-stimulating-hormone-receptor-polymorphisms-with-in-vitro-fertilization-outcomes
#7
Vijaya Ganesh, Vettriselvi Venkatesan, Teena Koshy, Sanjeeva Nellapalli Reddy, Suruli Muthumuthiah, Solomon Franklin Durairaj Paul
Despite the advances in in vitro fertilization (IVF), the implantation success rate for infertile women remains approximately only 15%. In this study, we sought to determine whether implantation failure after repeated IVF treatments is influenced by the presence of common variants in estrogen α, progesterone and follicle stimulating hormone receptor genes. The study population included three groups of women: group 1 were 50 women who had the transfer of ≥3 high-quality embryos during the IVF procedure without ever having had a clinical pregnancy; group 2 were 50 women who achieved a clinical pregnancy after ≤3 high-quality embryos transfers and group 3 were 50 control subjects who achieved a clinical pregnancy without any fertility therapy that resulted in a one live-born infant...
June 19, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29911897/per-and-polyfluoroalkyl-substances-impact-human-spermatogenesis-in-a-stem-cell-derived-model
#8
Alyse N Steves, Adam Turry, Brittany Gill, Danielle Clarkson-Townsend, Joshua M Bradner, Ian Bachli, W Michael Caudle, Gary W Miller, Anthony W S Chan, Charles A Easley
Per- and polyfluoroalkyl substances (PFASs) represent a highly ubiquitous group of synthetic chemicals used in products ranging from water and oil repellents and lubricants to firefighting foam. These substances can enter and accumulate in multiple tissue matrices in up to 100% of people assessed. Though animal models strongly identify these compounds as male reproductive toxicants, with exposed rodents experiencing declines in sperm count, alterations in hormones, and DNA damage in spermatids, among other adverse outcomes, human studies report conflicting conclusions as to the reproductive toxicity of these chemicals...
June 18, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29886763/inhibin-b-in-seminiferous-tubules-of-human-testes-in-normal-spermatogenesis-and-in-idiopathic-infertility
#9
Grigory A Demyashkin
Inhibin B is one of the most significant serum markers of spermatogenesis, but its testicular expression has been poorly studied. Inhibin B sensitivity as well as the ability to reflect the level and condition of spermatogenesis and forecast certain changes in reproductive homeostasis in males is still a subject of active discussion. The purpose of this study is to examine the level of expression of inhibin B in cells of human seminiferous tubules in normal and in pathological spermatogenesis (idiopathic infertility) by revealing the proportion of immunostaining cells for inhibin B...
June 9, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29873257/impact-of-prenatal-arsenic-exposure-on-chronic-adult-diseases
#10
Jamie L Young, Lu Cai, J Christopher States
Exposure to environmental stressors during susceptible windows of development can result in negative health outcomes later in life, a concept known as the Developmental Origins of Health and Disease (DOHaD). There is a growing body of evidence that exposures to metals early in life (in utero and postnatal) increase the risk of developing adult diseases such as cancer, cardiovascular disease, non-alcoholic fatty liver disease, and diabetes. Of particular concern is exposure to the metalloid arsenic, a drinking water contaminant and worldwide health concern...
June 6, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29806498/metabolomics-analysis-of-follicular-fluid-in-women-with-ovarian-endometriosis-undergoing-in-vitro-fertilization
#11
Abdullah Karaer, Gorkem Tuncay, Akın Mumcu, Berat Dogan
The purpose of this study was to investigate whether a change in the follicular fluid metabolomics profile due to endometrioma is identifiable. Twelve women with ovarian endometriosis (aged<40 years, with a body mass index [BMI] of <30 kg/m2 ) and 12 age- and BMI-matched controls (women with infertility purely due to a male factor) underwent ovarian stimulation for intracytoplasmic sperm injection (ICSI). Follicular fluid samples were collected from both of groups at the time of oocyte retrieval for ICSI...
May 28, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29798686/effects-of-saturated-palmitic-acid-and-omega-3-polyunsaturated-fatty-acids-on-sertoli-cell-apoptosis
#12
Xuechun Hu, Xie Ge, Wei Liang, Yong Shao, Jun Jing, Cencen Wang, Rong Zeng, Bing Yao
Obesity is believed to negatively affect male semen quality and is accompanied by dysregulation of free fatty acid (FFA) metabolism in plasma. However, the implication of dysregulated FFA on semen quality and the involvement of Sertoli cells remain unclear. In the present study, we report obesity decreased Sertoli cell viability through dysregulated FFAs. We observed an increased rate of apoptosis in Sertoli cells, accompanied with elevated FFA levels, in the testes of obese mice that were provided a high-fat diet (HFD)...
May 25, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29788785/impaired-serotonin-communication-during-juvenile-development-in-rats-diminishes-adult-sperm-quality
#13
Juan Díaz-Ramos, Maribel Flores-Flores, María E Ayala, Andrés Aragón-Martínez
Spermatogenesis and steroidogenesis are testicular functions regulated by gonadotrophins as well as other factors, including serotonin. Testicular serotonin acts as an autocrine regulator of testosterone secretion, but studies on its role in spermatogenesis and sperm quality are scarce. Here, we analyzed the effects of intratesticular inhibition of serotonin synthesis on gonadotrophins, testosterone, and sperm quality. Both testicles of 30-day-old rats were injected once with saline solution (SS) or distinct concentrations of p-chloroamphetamine (PCA) (0...
May 22, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29764212/the-clinical-application-of-single-sperm-based-snp-haplotyping-for-pgd-of-osteogenesis-imperfecta
#14
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
Osteogenesis imperfecta (OI) is a genetically heterogeneous disorder, presenting either autosomal dominant, autosomal recessive or X-linked inheritance patterns. The majority of OI cases are autosomal dominant and are caused by heterozygous mutations in either the COL1A1 or COL1A2 gene. In these dominant disorders, allele dropout (ADO) can lead to misdiagnosis in preimplantation genetic diagnosis (PGD). Polymorphic markers linked to the mutated genes have been used to establish haplotypes for identifying ADO and ensuring the accuracy of PGD...
May 15, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29737873/adamts1-and-adamts5-metalloproteases-produced-by-sertoli-cells-a-potential-diagnostic-marker-in-azoospermia
#15
Oya Sena Aydos, Yunus Yukselten, Sinan Ozkavukcu, Asuman Sunguroglu, Kaan Aydos
In this study, our aim was to detect protein levels of A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1 and 5 (ADAMTS1 and ADAMTS5) proteases and to examine the effect of in vitro FSH supplementation on protease production in cultured Sertoli cells. The expression of metalloproteases, ADAMTS1, and ADAMTS5 were investigated in Sertoli cell cultures as well as in ejaculate of azoospermic men which then were compared with ejaculates of the fertile control group. A total of 15 azoospermic men, diagnosed as obstructive (OA, n = 5) and nonobstructive (NOA, n = 10) azoospermia were included in the study...
May 8, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29718716/complex-chromosomal-aberrations-in-a-fetus-originating-from-oocytes-with-smooth-endoplasmic-reticulum-ser-aggregates
#16
Ioannis A Sfontouris, George T Lainas, Tryfon G Lainas, Efthimios Faros, Maria Banti, Katerina Kardara, Katerina Anagnostopoulou, Harris Kontos, George K Petsas, Efstratios M Kolibianakis
The presence of smooth endoplasmic reticulum aggregates (SERa) in the ooplasm is considered as the most severe oocyte dysmorphism due to its serious and potentially lethal outcomes in offspring. In the present case report, a couple underwent their first intracytoplasmic sperm injection (ICSI) cycle using a gonadotrophin releasing hormone (GnRH) antagonist protocol, followed by fetal ultrasound scanning and amniocentesis. SERa were observed in all oocytes retrieved. A singleton pregnancy was established. The second trimester fetal ultrasound scan revealed a female fetus with overlapping fingers in both hands, and amniocentesis was performed for the detection of chromosomal abnormalities...
May 2, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29696996/a-technical-assessment-of-the-porcine-ejaculated-spermatozoa-for-a-sperm-specific-rna-seq-analysis
#17
Marta Gòdia, Fabiana Quoos Mayer, Julieta Nafissi, Anna Castelló, Joan Enric Rodríguez-Gil, Armand Sánchez, Alex Clop
The study of the boar sperm transcriptome by RNA-seq can provide relevant information on sperm quality and fertility and might contribute to animal breeding strategies. However, the analysis of the spermatozoa RNA is challenging as these cells harbor very low amounts of highly fragmented RNA, and the ejaculates also contain other cell types with larger amounts of non-fragmented RNA. Here, we describe a strategy for a successful boar sperm purification, RNA extraction and RNA-seq library preparation. Using these approaches our objectives were: (i) to evaluate the sperm recovery rate (SRR) after boar spermatozoa purification by density centrifugation using the non-porcine-specific commercial reagent BoviPureTM ; (ii) to assess the correlation between SRR and sperm quality characteristics; (iii) to evaluate the relationship between sperm cell RNA load and sperm quality traits and (iv) to compare different library preparation kits for both total RNA-seq (SMARTer Universal Low Input RNA and TruSeq RNA Library Prep kit) and small RNA-seq (NEBNext Small RNA and TailorMix miRNA Sample Prep v2) for high-throughput sequencing...
April 26, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29658329/identification-of-a-novel-mutation-in-fgfr1-gene-in-patients-with-kallmann-syndrome-by-high-throughput-sequencing
#18
Bao-Fang Jin, Zhi-Yong Ji, Zhi-Ying Su, Li-Bin Mei, Xian-Jing Huang, Shao-Bin Lin, Ping Li, Yan-Wei Sha
Kallmann syndrome (KS) is a rare clinical and genetic heterogeneity disease, which is familial or sporadic. KS is known to have three patterns of inheritance: X linked recessive inheritance, autosomal dominant inheritance and rare autosomal recessive inheritance. Here, we report a sibling pedigree with autosomal dominant inheritance of KS, and we identified a novel heterozygous frameshift mutation c.299_300insCCGCAGACTCCGGCCTCTATGC (p.C101Rfs*17) in FGFR1 gene using whole-exome sequencing (WES). The mutation and affection status were cosegregated...
June 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29620421/adding-bovine-seminal-plasma-prior-to-freezing-improves-post-thaw-bull-sperm-kinematics-but-decreases-mitochondrial-activity
#19
Thanapol Nongbua, Essraa M Al-Essawe, Anders Edman, Anders Johannisson, Jane M Morrell
Variations in fertility between bulls with comparable sperm quality could be due to differences in their seminal plasma (SP). The aim of this study was to investigate the effect of adding bovine SP from bulls of known fertility to SP-free sperm samples. After removal of SP by Single Layer Centrifugation, resuspended sperm pellets were treated with SP from high or low fertility bulls at 0% (control), 1%, or 5% before freezing. Sperm quality was evaluated after thawing. Data were analyzed using Proc MIXED, SAS®...
June 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29595066/follicular-fluid-levels-of-anti-m%C3%A3-llerian-hormone-insulin-like-growth-factor-1-and-leptin-in-women-with-fertility-disorders
#20
Radek Kucera, Vaclav Babuska, Zdenka Ulcova-Gallova, Vlastimil Kulda, Ondrej Topolcan
Anti-Müllerian hormone (AMH), insulin-like growth factor 1 (IGF1) and leptin are produced in the granulosa cells of follicles and play an important role in the growth and maturation of follicles. The aim of our study was to monitor AMH, IGF1 and leptin levels in a group of healthy women and compare them to a group of women with fertility disorders. The second aim was the evaluation of biomarker levels in relation to the identified cause of infertility. Totally, 146 females were enrolled into our study. Seventy-two healthy controls and seventy-four females with fertility disorders were divided into four subgroups: anovulation, endometriosis, fallopian tube damage, unknown reason...
June 2018: Systems Biology in Reproductive Medicine
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