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Korean Journal of Laboratory Medicine

Young-Jin Lee
No abstract text is available yet for this article.
October 2011: Korean Journal of Laboratory Medicine
Hyewon Park, Jungwon Hyun, Sung Sup Park, Myoung Hee Park, Eun Young Song
Loss of heterozygosity (LOH) in chromosome 6p has been reported in a number of tumors and some hematologic malignancies, including ALL. LOH in chromosome 6p, on which the HLA genes are located, can give rise to false homozygosity results in HLA genotyping of patients with hematologic malignancies. Here we report false homozygosity results in HLA genotyping due to the loss of whole chromosome 6 in the neoplastic cells of a patient with ALL. A 33-yr-old Korean female patient was admitted for the evaluation of leukocytosis detected during a workup for headache...
October 2011: Korean Journal of Laboratory Medicine
Kyoung Ho Roh, Chang Kyu Lee, Jang Wook Sohn, Wonkeun Song, Dongeun Yong, Kyungwon Lee
Carbapenem-resistant Klebsiella pneumoniae isolates producing K. pneumoniae carbapenemases (KPC) were first reported in the USA in 2001, and since then, this infection has been reported in Europe, Israel, South America, and China. In Korea, the first KPC-2-producing K. pneumoniae sequence type (ST) 11 strain was detected in 2010. We report the case of a patient with a urinary tract infection caused by KPC-2-producing K. pneumoniae. This is the second report of a KPC-2-producing K. pneumoniae infection in Korea, but the multilocus sequence type was ST258...
October 2011: Korean Journal of Laboratory Medicine
Dae-Hyun Ko, Ho Eun Chang, Sang Hoon Song, Hoil Yoon, Kyoung Un Park, Junghan Song
Alpha 1-antitrypsin (AAT) deficiency is a genetic disorder that primarily affects the lungs and liver. While AAT deficiency is one of the most common genetic disorders in the Caucasian population, it is extremely rare in Asians. Here, we report the case of a 36-year-old Korean woman with AAT deficiency who visited the emergency department of our hospital for the treatment of progressive dyspnea that had begun 10 years ago. She had never smoked. Chest computed tomography revealed panlobular emphysema in both lungs, which suggested AAT deficiency...
October 2011: Korean Journal of Laboratory Medicine
Jong Ho Lee, Hee Soon Cho, Myung Soo Hyun, Hwa-Young Kim, Hee-Jin Kim
Factor XI (FXI) deficiency is a rare autosomal recessive coagulation disorder most commonly found in Ashkenazi and Iraqi Jews, but it is also found in other ethnic groups. It is a trauma or surgery-related bleeding disorder, but spontaneous bleeding is rarely seen. The clinical manifestation of bleeding in FXI deficiency cases is variable and seems to poorly correlate with plasma FXI levels. The molecular pathology of FXI deficiency is mutation in the F11 gene on the chromosome band 4q35. We report a novel mutation of the F11 gene in an 18-year-old asymptomatic Korean woman with mild FXI deficiency...
October 2011: Korean Journal of Laboratory Medicine
Nae Yu Kim, Soo Jung Gong, Jimyung Kim, Seon Min Youn, Jung-Ae Lee
We report a rare case of multiple myeloma with biclonal gammopathy (IgG kappa and IgA lambda type) in a 58-year-old man with prostate cancer who presented with lower back pain. Through computed tomography (CT) imaging, an osteolytic lesion at the L3 vertebra and an enhancing lesion of the prostate gland with multiple lymphadenopathies were found. In the whole body positron emission tomography-computed tomography (PET-CT), an additional osteoblastic bone lesion was found in the left ischial bone. A prostate biopsy was performed, and adenocarcinoma was confirmed...
October 2011: Korean Journal of Laboratory Medicine
Mi Hee Jang, Go Eun Choi, Bo-Moon Shin, Seon Ho Lee, Sung-Ryul Kim, Chulhun L Chang, Jeong-Man Kim
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied...
October 2011: Korean Journal of Laboratory Medicine
Soo-Young Kim, Yeon-Joon Park, Jin Kyung Yu, Yeong Sic Kim
The aminoglycoside 6'-N-acetyltransferases of type Ib (aac(6')-Ib) gene confers resistance to amikacin, tobramycin, kanamycin, and netilmicin but not gentamicin. However, some isolates harboring this gene show reduced susceptibility to amikacin. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommends a revision of the phenotypic description for isolates harboring the aac(6')-Ib gene. In this study, we determined the aminoglycoside susceptibility profiles of 58 AAC(6')-Ib-producing Enterobacter cloacae isolates...
October 2011: Korean Journal of Laboratory Medicine
Kyeong-Hee Kim, Sung-Won Lee, Won-Tae Chung, Byoung-Gwon Kim, Kwang-Sook Woo, Jin-Yeong Han, Jeong-Man Kim
BACKGROUND: We assessed the efficacy of serial interferon-gamma release assays (IGRAs) for the diagnosis of latent tuberculosis infection (LTBI) in patients receiving immunosuppressive agents for treatment of rheumatic diseases in Korea. METHODS: Of 276 patients who underwent consecutive screening with one of two IGRAs [QuantiFERON-TB Gold or QuantiFERON-TB Gold In-Tube], 66 patients were evaluated by the serial IGRA for detection of LTBI during therapy with immunosuppressive agents...
October 2011: Korean Journal of Laboratory Medicine
Ji Youn Sung, Kye Chul Kwon, Hye Hyun Cho, Sun Hoe Koo
BACKGROUND: Members of the Acinetobacter calcoaceticus-baumannii (Acb) complex are important opportunistic bacterial pathogens and present significant therapeutic challenges in the treatment of nosocomial infections. In the present study, we investigated the integrons and various genes involved in resistance to carbapenems, aminoglycosides, and fluoroquinolones in 56 imipenem-nonsusceptible Acb complex isolates. METHODS: This study included 44 imipenem-nonsusceptible A...
October 2011: Korean Journal of Laboratory Medicine
Haeng Soon Jeong, Il Kwon Bae, Jeong Hwan Shin, Hee Jung Jung, Si Hyun Kim, Ja Young Lee, Seung Hwan Oh, Hye Ran Kim, Chulhun Ludgerus Chang, Weon-Gyu Kho, Jeong Nyeo Lee
BACKGROUND: We investigated the prevalence of plasmid-mediated quinolone resistance and its association with extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase in Enterobacteriaceae. METHODS: A total of 347 non-duplicated isolates of Enterobacteriaceae were collected between August and October 2006 from 2 hospitals. Qnr determinant screening was conducted using PCR amplification, and all positive results were confirmed by direct sequencing. Qnr-positive strains were determined on the basis of the presence of ESBL and AmpC beta-lactamase genes...
October 2011: Korean Journal of Laboratory Medicine
Minje Han, Sun-Hee Jun, Sang Hoon Song, Kyoung Un Park, Jin Q Kim, Junghan Song
BACKGROUND: We evaluated the performance of multiplex tandem mass spectrometry (MS/MS) in newborn screening for detection of 6 lysosomal storage disorders (LSDs), namely, Niemann-Pick A/B, Krabbe, Gaucher, Fabry, and Pompe diseases and Hurler syndrome. METHODS: We revised the conditions and procedures of multiplex enzyme assay for the MS/MS analysis and determined the precision of our enzyme assay and the effects of sample amounts and incubation time on the results...
October 2011: Korean Journal of Laboratory Medicine
Han Kyul Kim, Seung Jung Kee, Ji Yeon Seo, Eun Mi Yang, Hong Jae Chae, Chan Jong Kim
BACKGROUND: Gonadotropin-releasing hormone (GnRH) stimulation test is the gold standard to identify central precocious puberty (CPP). This test requires multiple blood samples at different time points to measure gonadotropin levels, and is therefore expensive, time-consuming, and uncomfortable for patients. We aimed to simplify the GnRH stimulation test to require fewer blood samples. METHODS: A study of 166 girls with precocious puberty was undertaken. Blood samples were obtained at 0, 15, 30, 45, 60, 90, and 120 min after GnRH administration, and the levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured...
October 2011: Korean Journal of Laboratory Medicine
Ji-Eun Kim, Bo-Ram Kim, Kwang-Sook Woo, Jeong-Man Kim, Joo-In Park, Jin-Yeong Han
BACKGROUND: β-thalassemia is primarily found in individuals of Mediterranean and Southeast Asian ancestry. With rapid growth in the Southeast Asian segments of the Korean population, the geographic distribution of hemoglobinopathies is expected to become significantly different from what it is today. In this study, Hb fractions were measured in patients with hypochromic microcytosis to detect thalassemia and Hb variants. To evaluate the feasibility of replacing cellulose acetate electrophoresis (CA) with capillary electrophoresis (CE) in a clinical laboratory, both techniques were performed and the outcomes were compared...
October 2011: Korean Journal of Laboratory Medicine
Hee-Won Moon, Bo Hyun Kim, Chul Min Park, Mina Hur, Yeo-Min Yun, Sung-Yong Kim, Mark Hong Lee
BACKGROUND: CD4+CD25+ regulatory T-cells (Tregs) play a critical role in immune responses. We explored the status of Tregs in neoplastic and autoimmune hematologic diseases. We also evaluated the technical aspects of Treg measurement in terms of sample type and detection markers. METHODS: A total of 68 subjects were enrolled: 11 with AML, 8 with MDS, 10 with autoimmune diseases, and 39 controls. Tregs were analyzed in peripheral blood (PB) and bone marrow (BM) samples from each subject...
October 2011: Korean Journal of Laboratory Medicine
Young-Uk Cho, Hyun-Sook Chi, Chan-Jeoung Park, Seongsoo Jang, Eul-Ju Seo, Cheolwon Suh
BACKGROUND: Myelomatous pleural effusion (MPE) is rare in myeloma patients. We present a consecutive series of patients with MPE in a single institution. METHODS: We retrospectively reviewed the medical records of 19 patients diagnosed with MPE between 1989 and 2008 at the Asan Medical Center. Diagnoses were confirmed by cytologic identification of malignant plasma cells in the pleural fluid. RESULTS: Our patients showed dominance of IgA (36...
October 2011: Korean Journal of Laboratory Medicine
Haiyoung Jung, Chang-Seok Ki, Won-Jung Koh, Kang-Mo Ahn, Sang-Il Lee, Jeong-Ho Kim, Jae Sung Ko, Jeong Kee Seo, Seung-Ick Cha, Eun-Sil Lee, Jong-Won Kim
BACKGROUND: Cystic fibrosis (CF) is one of the most common hereditary disorders among Caucasians. The most common mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been well established among Caucasian populations. In Koreans, however, there are very few cases of genetically confirmed CF thus far, and the spectrum of mutations seems quite different from that observed in Caucasians. METHODS: In the present study, we describe the cases of 2 Korean CF patients, present sequencing results identifying mutations in their CFTR gene, and summarize the results of CFTR mutational spectrum from previously reported Korean CF patients...
July 2011: Korean Journal of Laboratory Medicine
Hee Soon Cho, In Hwan Song, So-Young Park, Min Cheol Sung, Myun-Whan Ahn, Kyung Eun Song
BACKGROUND: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs). METHODS: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors...
July 2011: Korean Journal of Laboratory Medicine
Hee Jae Huh, Kyoung-Jin Park, Ja-Hyun Jang, Mina Lee, Jang Ho Lee, Yoon Hee Ahn, Cheol-In Kang, Chang-Seok Ki, Nam Yong Lee
Streptococcus suis infection is an emerging zoonosis in Asia. The most common disease manifestation is meningitis, which is often associated with hearing loss and cochleovestibular signs. S. suis infection in humans mainly occurs among risk groups that have frequent exposure to pigs or raw pork. Here, we report a case of S. suis meningitis in a 67-yr-old pig carcass handler, who presented with dizziness and sensorineural hearing loss followed by headaches. Gram-positive diplococci were isolated from cerebrospinal fluid (CSF) and blood cultures and showed gray-white colonies with α-hemolysis...
July 2011: Korean Journal of Laboratory Medicine
Jonghyeon Choi, Yangsoon Lee, Hae-Sun Chung, Ja-Seung Koo, Dongeun Yong, Yu Sun Kim, Kyungwon Lee, Yunsop Chong
Phaeohyphomycosis is a subcutaneous infection caused by dark pigmented fungi, including fungi of the species Phaeoacremonium, Alternaria, Exophiala, and Pyrenochaeta. In August 2005, a 54-yr-old man who had received a renal transplant 5 yr ago was admitted to our hospital with a subcutaneous mass on the third finger of the right hand; the mass had been present for several months. He had been receiving immunosuppressive agents for several years. He underwent excision of the mass, which was followed by aspiration of the wound for bacterial and fungal cultures...
July 2011: Korean Journal of Laboratory Medicine
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