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Journal of Biophotonics

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https://www.readbyqxmd.com/read/28921863/gender-differences-in-brain-networks-during-verbal-sternberg-tasks-a-simultaneous-near-infrared-spectroscopy-and-electro-encephalography-study
#1
Chenyang Gao, Jinyan Sun, Xiaoquan Yang, Hui Gong
Gender differences in psychological processes have been of great interest in a variety of fields including verbal fluency, emotion processing, and working memory. Previous studies suggested that women outperform men in verbal working memory (VWM). However, the inherent mechanisms are still unclear. To obtain a deeper insight into the gender differences in brain networks in VWM, this study used near-infrared spectroscopy (NIRS) and electro-encephalography (EEG) simultaneously to investigate gender-related brain networks during verbal Sternberg tasks...
September 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28921845/detection-and-delineation-of-squamous-neoplasia-with-hyperspectral-imaging-in-a-mouse-model-of-tongue-carcinogenesis
#2
Guolan Lu, Dongsheng Wang, Xulei Qin, Susan Muller, Xu Wang, Amy Y Chen, Zhuo Georgia Chen, Baowei Fei
yperspectral imaging (HSI) holds the potential for the noninvasive detection of cancers. Oral cancers are often diagnosed at a late stage when treatment is less effective and the mortality and morbidity rates are high. Early detection of oral cancer is, therefore, crucial in order to improve the clinical outcomes. To investigate the potential of HSI as a non-invasive diagnostic tool, an animal study was designed to acquire hyperspectral images of in vivo and ex vivo mouse tongues from a chemically induced tongue carcinogenesis model...
September 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28869350/utilising-lymphatic-cell-markers-to-visualise-human-lymphatic-abnormalities
#3
REVIEW
Zerina Lokmic
The in vivo visualisation of the human lymphatic system is limited by the mode of delivery of tracing agents, depth of field and size of the area examined, and specificity of the cell markers used to distinguish lymphatic endothelium from the blood vessels and the surrounding tissues. These limitations are particularly problematic when imaging human lymphatic abnormalities. Firstly, limited understanding of the lymphatic disease aetiology exists with respect to genetic causes and phenotypic presentations. Secondly, the ability of a tracer to reach the entire lymphatic network within the diseased tissue is suboptimal...
September 4, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28858435/in-vivo-multiphoton-microscopy-using-a-handheld-scanner-with-lateral-and-axial-motion-compensation
#4
Ben Sherlock, Sean C Warren, Yuriy Alexandrov, Fei Yu, James Stone, Jonathan Knight, Mark A A Neil, Carl Paterson, Paul M W French, Chris Dunsby
This paper reports a handheld multiphoton fluorescence microscope designed for clinical imaging that incorporates axial motion compensation and lateral image stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Our system is able to correct lateral sample velocities of up to ~65 μm s(-1) . Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications...
August 31, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28858432/imaging-lymphatics-in-human-normal-and-lymphedema-limbs-usefulness-of-various-modalities-for-evaluation-of-lymph-and-edema-fluid-flow-pathways-and-dynamics
#5
Marzanna T Zaleska, Waldemar L Olszewski
The human lymphatic system morphology and function still remain largely unknown to clinicians and biologists. How does the lymphatic vascular system look like in comparison to the blood transport system, how does lymph flow, where does capillary filtrate accumulate in cases with lymphatic obstruction caused by inflammation, trauma and cancer therapy, remain as basic questions. Visualization of the lymphatic pathways and dynamics of lymph flow, and in cases of obstruction, the localization of the capillary filtrate/edema fluid accumulation becomes indispensable...
August 31, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28853237/pixel-classification-method-in-optical-coherence-tomography-for-tumor-segmentation-and-its-complementary-usage-with-oct-microangiography
#6
Alexander Moiseev, Ludmila Snopova, Sergey Kuznetsov, Natalia Buyanova, Vadim Elagin, Marina Sirotkina, Elena Kiseleva, Lev Matveev, Vladimir Zaytsev, Felix Feldchtein, Elena Zagaynova, Valentin Gelikonov, Natalia Gladkova, Alex Vitkin, Grigory Gelikonov
A novel machine-learning method to distinguish between tumor and normal tissue in optical coherence tomography (OCT) has been developed. Pre-clinical murine ear model implanted with mouse colon carcinoma CT-26 was used. Structural-image-based feature sets were defined for each pixel and machine learning classifiers were trained using "ground truth" OCT images manually segmented by comparison with histology. The accuracy of the OCT tumour segmentation method was then quantified by comparing with fluorescence imaging of tumors expressing genetically encoded fluorescent protein KillerRed that clearly delineates tumor borders...
August 29, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28851134/cnn-based-malaria-diagnosis-from-focus-stack-of-blood-smear-images-acquired-using-custom-built-slide-scanner
#7
G Gopakumar, M Swetha, Sai Siva Gorthi, Gorthi R K Sai Subrahmanyam
This paper introduces a focus stacking based approach for automated quantitative detection of Plasmodium falciparum malaria from blood smear. For the detection, a custom designed convolutional neural network (CNN) operating on focus stack of images is used. The cell counting problem is addressed as the segmentation problem and we propose a two level segmentation strategy. Use of CNN operating on focus stack for the detection of malaria is first of its kind, and it not only improved the detection accuracy (both in terms of sensitivity (97...
August 29, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28851128/current-status-pitfalls-and-future-directions-in-the-diagnosis-and-therapy-of-lymphatic-malformation
#8
REVIEW
Ravi W Sun, Valery V Tuchin, Vladimir P Zharov, Ekaterina I Galanzha, Gresham T Richter
Lymphatic malformations (LM) are complex congenital vascular lesions composed of dilated, abnormal lymphatic channels of varying size that can result in significant aesthetic and physical impairment due to relentless growth. LMs comprised of micro-lymphatic channels (microcystic) integrate and infiltrate normal soft tissue, leading to a locally invasive mass. Ultrasonography (US) and magnetic resonance imaging (MRI) assist in the diagnosis but are unable to detect microvasculature present in microcystic LM...
August 29, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28837261/detection-of-free-oxygen-and-water-vapour-in-fertilized-and-unfertilized-eggs-by-diode-laser-spectroscopy-exploration-of-diagnostics-possibilities
#9
Wansha Li, Huiying Lin, Hao Zhang, Katarina Svanberg, Sune Svanberg
Non-intrusive methods for characterizing food products are of increasing interest related to the greater awareness of food safety issues. Hen eggs are an important part in food consumption in most parts of the world. We have investigated an optical method utilizing tunable diode lasers for monitoring free gas in eggs. We show that oxygen signals, recorded around 760 nm, increase steadily as eggs become older. Further, we investigated fertilized eggs, which show a quite different temporal behavior during the hatching time...
August 24, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28834287/partial-nephrectomy-margin-imaging-using-structured-illumination-microscopy
#10
Mei Wang, David B Tulman, Andrew B Sholl, Sree H Mandava, Michael M Maddox, Benjamin R Lee, J Quincy Brown
Partial nephrectomy (PN) is the recommended procedure over radical nephrectomy (RN) for patients with renal masses < 4 cm in diameter (Stage T1a). Patients with > 4 cm renal masses can also be treated with PN, but have a higher risk for positive surgical margins. Positive surgical margins (PSM), when present, are indicative of poor clinical outcomes. The current gold-standard histopathology method is not well-suited for the identification of PSM intra-operatively due to processing time and destructive nature...
August 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28816398/optical-properties-of-mice-s-stool-in-550-1000-nm-wavelength-range
#11
Helene Isler, Catherine Germanier, Linda Ahnen, Jingjing Jiang, Scott Lindner, Aldo Di Costanzo Mata, Tanja Karen, Salvador Sánchez Majos, Martin Wolf, Alexander Kalyanov
The aim of this work was to measure optical properties of stool of mice to provide this relevant wavelength-dependent behavior for optical imaging modalities such as fluorescent molecular tomography and near-infrared optical tomography. BALB/c nude female mice were studied and optical properties of the stool were determined by employing the inverse adding-doubling approach. The animals were kept on chlorophyll-free diet. Nine stool samples were measured. The wavelength-dependent behavior of absorption and scattering in 550-1000 nm range is presented...
August 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28816029/development-of-a-3d-tissue-lung-phantom-of-a-preterm-infant-for-optical-measurements-of-oxygen-laser-detector-position-considerations
#12
Jim Larsson, Peilang Liao, Patrik Lundin, Emilie Krite Svanberg, Johannes Swartling, Märta Lewander Xu, Joakim Bood, Stefan Andersson-Engels
There is a need for further improving the clinical care of our most vulnerable patients - preterm infants. Novel diagnostic and treatment tools facilitate such advances. Here we are evaluating a potential percutaneous optical monitoring tool to assess the oxygen and water vapor contents in the lungs of preterm babies. The aim is to prepare for further clinical studies by gaining detailed understanding of how the measured light intensity and gas absorption signal behave for different possible geometries of the light delivery and receiver...
August 16, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28802090/assessment-of-the-dynamics-of-human-glymphatic-system-by-near-infrared-spectroscopy-nirs
#13
Teemu Myllylä, Markus Harju, Vesa Korhonen, Alexander Bykov, Vesa Kiviniemi, Igor Meglinski
Fluctuations in brain water content has attracted increasing interest, particularly as regards studies of the glymphatic system, which is connected with the complex organisation of dural lymphatic vessels, responsible for cleaning tissue. Disturbances of glymphatic circulation are associated with several brain disorders, including dementia. This paper introduces an approach to non-invasive measurement of water dynamics in the human brain utilizing near-infrared spectroscopy (NIRS). We demonstrate the possibility to sense dynamic variations of water content between the skull and grey matter, for instance, in the subarachnoid space...
August 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28800386/3d-correlative-single-cell-imaging-utilizing-fluorescence-and-refractive-index-tomography
#14
Mirjam Schürmann, Gheorghe Cojoc, Salvatore Girardo, Elke Ulbricht, Jochen Guck, Paul Müller
Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D-correlated RI and fluorescence data for a quantitative interpretation of the nuclear RI...
August 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28800207/optically-derived-metabolic-and-hemodynamic-parameters-predict-hippocampal-neurogenesis-in-the-btbr-mouse-model-of-autism
#15
David Abookasis, Danit Lerman, Hava Roth, Mati Tfilin, Gadi Turgeman
In the present study, we made use of dual-wavelength laser speckle imaging (DW-LSI) to assess cerebral blood flow in the BTBR genetic mouse model of Autism Spectrum Disorder, as well as control (C57Bl/6J) mice. Since the deficits in social behavior demonstrated by BTBR mice are attributed to changes in neural tissue structure and function, we postulated that these changes can be detected optically using DW-LSI. BTBR mice demonstrated reductions in both cerebral blood flow (CBF) and cerebral oxygen metabolism (CMRO2 ), as suggested by studies using conventional neuroimaging technologies to reflect impaired neuronal activation and cognitive function...
August 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28800205/wide-field-color-imaging-of-scatter-based-tissue-contrast-using-both-high-spatial-frequency-illumination-and-cross-polarization-gating
#16
Mackenzie L Carlson, David M McClatchy, Jason R Gunn, Jonathan T Elliott, Keith D Paulsen, Stephen C Kanick, Brian W Pogue
This study characterizes the scatter-specific tissue contrast that can be obtained by high spatial frequency (HSF) domain imaging and cross-polarization (CP) imaging, using a standard color imaging system, and how combining them may be beneficial. Both HSF and CP approaches are known to modulate the sensitivity of epi-illumination reflectance images between diffuse multiply-scattered and superficially backscattered photons, providing enhanced contrast from microstructure and composition than what is achieved by standard wide-field imaging...
August 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28792669/an-improved-algorithm-for-fast-resonant-mie-scatter-correction-of-infrared-spectra-of-cells-and-tissues
#17
Tatiana Konevskikh, Rozalia Lukacs, Achim Kohler
Mie scattering effects create serious problems for the interpretation of FTIR spectra of single cells and tissues. During recent years, different techniques were proposed to retrieve pure absorbance spectra from spectra with Mie distortions. Recently, we published an iterative algorithm for correcting Mie scattering in spectra of single cells and tissues, which we called 'the fast resonant Mie scatter correction algorithm'. The algorithm is based on extended multiplicative signal correction (EMSC) and employs a meta-model for a parameter range of refractive index and size parameters...
August 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28787543/endomicroscopic-optical-coherence-tomography-for-cellular-resolution-imaging-of-gastrointestinal-tracts
#18
Yuemei Luo, Dongyao Cui, Xiaojun Yu, En Bo, Xianghong Wang, Nanshuo Wang, Cilwyn Shalitha Braganza, Shufen Chen, Xinyu Liu, Qiaozhou Xiong, Si Chen, Shi Chen, Linbo Liu
Our ability to detect neoplastic changes in gastrointestinal (GI) tracts is limited by the lack of an endomicroscopic imaging tool that provides cellular-level structural details of GI mucosa over a large tissue area. In this paper, we report a fiber-optic based micro-optical coherence tomography (μOCT) system and demonstrate its capability to acquire cellular-level details of GI tissue through circumferential scanning. The system achieves an axial resolution of 2.48 µm in air and a transverse resolution of 4...
August 8, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28787111/nonlinear-optoacoustic-readings-from-diffusive-media-at-near-infrared-wavelengths
#19
Jaber Malekzadeh-Najafabadi, Jaya Prakash, Vasilis Ntziachristos
Optoacoustic (photoacoustic) imaging assumes that the detected signal varies linearly with laser energy. However, non-linear intensity responses as a function of light fluence have been suggested in optoacoustic microscopy, i.e within the first millimeter of tissue. In this work, we explore the presence of nonlinearity deeper in tissue (~4 mm), as it relates to optoacoustic mesoscopy, and investigate the fluence required to delineate a switch from linear to non-linear behavior. Optoacoustic signal non-linearity is studied for different materials, different wavelengths and as a function of changes in the scattering and absorption coefficient of the medium imaged...
August 8, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28772008/dual-wavelength-reflectance-spectroscopy-of-the-superior-vena-cava-a-method-for-placing-central-venous-catheters-at-the-cavoatrial-junction
#20
Bradley J Stringer, Spencer B Shumway, Matthew D Holbrook, Cameron G Copeland, Alexander A Gosch, Kai Kück
There are a limited number of methods to guide and confirm the placement of a peripherally-inserted central catheter (PICC) at the cavoatrial junction. The aim of this study was to design, test, and validate a dual wavelength, diode laser-based, single optical fiber instrument that would accurately confirm PICC tip location at the cavoatrial junction of an animal heart, in vivo. This was accomplished by inserting the optical fiber into a PICC and ratiometrically comparing simultaneous visible and near-infrared reflection intensities of venous and atrial tissues found near the cavoatrial junction...
August 3, 2017: Journal of Biophotonics
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