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Journal of Biophotonics

E Baria, G Nesi, R Santi, V Maio, D Massi, C Pratesi, R Cicchi, F S Pavone
Coronary heart disease (CHD) is the most common type of heart disease, and it's caused by atherosclerosis. In fact, an arterial wall lesion centered on the accumulation of cholesterol-rich lipids and the accompanying inflammatory response generates a plaque, whose rupture may result in a thrombus with fatal consequences. Plaque characterization for assessing the severity of atherosclerosis is generally performed through standard histopathological examination based on haematoxylin/eosin (H&E) staining, which is operator-dependent and requires relatively long procedures...
June 22, 2018: Journal of Biophotonics
Andrea Amaroli, Manuela Marcoli, Arianna Venturini, Mario Passalacqua, Luigi F Agnati, Alberico Benedicenti, Mirco Raffetto, Guido Maura, Stefano Benedicenti, Chiara Cervetto
Although photons have been repeatedly shown to affect the functioning of the nervous system, their effects on neurotransmitter release have never been investigated. We exploited in vitro models that allow effects involving neuron-astrocyte network functioning to be detected (mouse cerebrocortical slices) and dissected these effects at cerebrocortical nerve endings and astrocyte processes. Infrared light proved able to induce glutamate release by stimulating glutamatergic nerve endings. This article is protected by copyright...
June 22, 2018: Journal of Biophotonics
Jun Xia, Weihui Zeng, Yumin Xia, Bingchuan Wang, Dehui Xu, Dingxin Liu, Michael G Kong, Yingying Dong
Cold atmospheric plasma (CAP) represents a promising therapy for selectively cancer killing. However, the mechanism of CAP-induced cancer cell death remains unclear. Here, we identified the tumor necrosis factor (TNF)-family members, especially Fas, and overloaded intracellular nitric oxide (NO) participated in CAP induced apoptosis in A375 and A875 melanoma cell lines, which was known as extrinsic apoptosis pathway. This progress was mediated by antagonistic protein of reactive oxygen species (ROS), Sestrin2...
June 22, 2018: Journal of Biophotonics
Wenjun Wang, Joseph P Miller, Susan C Pannullo, Cynthia A Reinhart-King, Francois Bordeleau
Cell contractility regulates multiple cell behaviors which contribute to both normal and pathological processes. However, measuring cell contractility remains a technical challenge in complex biological samples. The current state of the art technologies employed to measure cell contractility have inherent limitations that greatly limit the experimental conditions under which they can be used. Here, we use quantitative polarization microscopy to extract information about cell contractility. We show that the optical retardance signal measured from the cell body is proportional to cell contractility in two-dimensional and three-dimensional platforms, and as such can be used as a straightforward, tractable methodology to assess cell contractility in a variety of systems...
June 22, 2018: Journal of Biophotonics
Chiu-Mei Hsueh, Hung-Ming Lin, Te-Yu Tseng, Yao-De Huang, Hsuan-Shu Lee, Chen-Yuan Dong
Second order susceptibility (SOS) microscopy is used to image and characterize chondrogenesis in cultured human mesesnchymal stem cells (hMSCs). SOS analysis shows that the second order susceptibility tensor ratios can be used to characterize type I and II collagens in living tissues and that both collagen types are produce at the onset of chondrogenesis. Time-lapse analysis shows a modulation of extracellular matrix results in an higher rate in increase of type II collagen, as compared to type I collagen. With time, type II collagen content stabilizes at the composition of 70% of total collagen content...
June 19, 2018: Journal of Biophotonics
Michael A Taylor, Gilles C Vanwalleghem, Itia A Favre-Bulle, Ethan K Scott
Light-sheet microscopy is used extensively in developmental biology and neuroscience. One limitation of this approach is that absorption and scattering produces shadows in the illuminating light sheet, resulting in stripe artifacts. Here, we introduce diffuse light-sheet microscopes that use a line diffuser to randomize the light propagation within the image plane, allowing the light sheets to reform after obstacles. We incorporate diffuse light sheets in two existing configurations: selective plane illumination microscopy (SPIM) in which the sample is illuminated with a static sheet of light, and digitally scanned light sheet (DSLS) in which a thin Gaussian beam is scanned across the image plane during each acquisition...
June 19, 2018: Journal of Biophotonics
Wei-Tang Lin, Chen Yen Lin, Vijay Raj Singh, Yuan Luo
Optical sectioning endoscopy such as confocal endoscopy offers capabilities to obtain three-dimensional (3D) information from various biological samples by discriminating between the desired in-focus signals and out-of-focus background. However, in general confocal images are formed through point-by-point scanning and the scanning time is proportional to the 3D space-bandwidth product. Recently, structured illumination endoscopy has been utilized for optically sectioned wide-field imaging, but it still needs axial scanning to acquire images from different depths of focal plane...
June 19, 2018: Journal of Biophotonics
Peijun Gong, Dao-Yi Yu, Qiang Wang, Paula Yu, Karol Karnowski, Morgan Heisler, Ashley Francke, Dong An, Marinko V Sarunic, David D Sampson
We employ optical coherence tomography (OCT) and optical coherence microscopy (OCM) to study conjunctival lymphatics in porcine eyes ex vivo. This study is a precursor to the development of in vivo imaging of the collecting lymphatics for potentially guiding and monitoring glaucoma filtration surgery. OCT scans at 1300 nm and higher-resolution OCM scans at 785 nm reveal the lymphatic vessels via their optical transparency. Equivalent signal characteristics are also observed from blood vessels largely free of blood (and devoid of flow) in the ex vivo conjunctiva...
June 19, 2018: Journal of Biophotonics
Itay Remer, Lorraine F Pierre-Destine, David Tay, Linnie M Golightly, Alberto Bilenca
Cerebral malaria is a severe complication of Plasmodium falciparum infection associated with impaired cerebral blood flow. Visualization of the eye vasculature, which is embryologically derived from that of the brain, is used clinically to diagnose the syndrome. Here, we introduce camera-phone laser speckle imaging as a new tool for in-vivo, noncontact two-dimensional mapping of blood flow dynamics in the experimental cerebral malaria (ECM) murine model of Plasmodium berghei ANKA. In a longitudinal study, we show that the camera-phone imager can detect an overall decrease in the retinal blood-flow-speed as ECM develops in Plasmodium berghei ANKA infected mice, with no similar change observed in uninfected control mice or mice infected with a non-ECM inducing strain (Plasmodium berghei NK65)...
June 14, 2018: Journal of Biophotonics
Irina Yu Yanina, Yulia I Svenskaya, Ekaterina S Prikhozhdenko, Daniil N Bratashov, Maria V Lomova, Dmitry A Gorin, Gleb B Sukhorukov, Valery V Tuchin
Enzymatic destruction of adipose tissue has been achieved by encapsulation of lipase into the polymeric microcapsules. Adipose tissue destruction was delayed while lipase is encapsulated comparing to the direct lipase action as demonstrated by optical microscopy and optical coherence tomographyin in vitro studies. Raman spectroscopy confirms that triglycerides in fat tissue were cleaved into free fatty acids, glycerol and possible di- and monoglyceride residues. The results underpin the concept of local and controlled treatment of tissues via encapsulation...
June 13, 2018: Journal of Biophotonics
Will J Eldridge, Jawad Hoballah, Adam Wax
Apoptotic mechanisms are often dysregulated in cancerous phenotypes. Additionally, many anticancer treatments induce apoptosis and necrosis, and the monitoring of this apoptotic activity can allow researchers to identify therapeutic efficiency. Here we introduce a microscope which combines quantitative phase imaging with the ability to detect molecular events via FRET. The system was applied to study cells undergoing apoptosis to correlate the onset of apoptotic enzyme activity as observed using a FRET based apoptosis sensor with whole cell morphological changes analyzed via QPI...
June 13, 2018: Journal of Biophotonics
Lutz Schmitz, Schapoor Hessam, Lisa Scholl, Sarah Reitenbach, Marc Hanno Segert, Thilo Gambichler, Eggert Stockfleth, Falk Georges Bechara
Actinic keratoses (AKs) can progress into invasive squamous cell carcinoma and thus may become a life threatening disease. Argon plasma coagulation (APC) might complement the therapeutic armamentarium in particular for AK lesions. However, there is no data on APC induced micromorphological changes following the treatment of AKs. We aimed to determine in-vivo APC induced effects on the epidermis and dermo-epidermal junction zone (DEJ) in AK lesions. We performed APC in 108 AKs using the spray mode with a power setting of 15W and a flow rate of 2...
June 12, 2018: Journal of Biophotonics
Uma Keyal, Qiong Luo, Anil Kumar Bhatta, Hansen Luan, Ping Zhang, Qianqian Wu, Haiyan Zhang, Pei Liu, Linglin Zhang, Peiru Wang, Degang Yang, Guolong Zhang, Jing Yao, Xiuli Wang, Lei Shi
Zinc Pthalocyanine (ZnPc) is a second- generation photodynamic therapy (PDT) sensitizer with sufficient PDT activity for squamous cell carcinoma (SCC). ZnPc is hydrophobic and insoluble in water, which creates hurdles in systemic administration and hence restricts its use in clinic. Here we have loaded ZnPc on chitosan / methoxy polyethylene glycol - polylactic acid (CPP) nanoparticles to form Z-CPP to enhance PDT efficacy. In vitro and in vivo studies were performed to see dark toxicity of the compounds ZnPc, CPP, and Z-CPP...
June 12, 2018: Journal of Biophotonics
Liyi Huang, Weijun Xuan, Tadeusz Sarna, Michael R Hamblin
We have previously shown that antimicrobial photodynamic therapy (aPDT) mediated by different photosensitizers can be potentiated by a variety of inorganic salts. Potassium thiocyanate potentiated aPDT mediated by methylene blue (MB), while potassium selenocyanate potentiated aPDT mediated by MB, Rose Bengal and the anionic porphyrin TPPS4. However, the mechanisms of action that were proposed were fundamentally different. In the present study we compare these two salts (KSCN and KSeCN) with different light-activated photosensitizers (PS) and different oxidative reactions for killing Gram-positive and Gram-negative bacteria...
June 8, 2018: Journal of Biophotonics
Roger Bresolí-Obach, Ignacio Gispert, Diego García Peña, Sonia Boga, Óscar Gulias, Montserrat Agut, M Eugenio Vázquez, Santi Nonell
Light-mediated killing of pathogens by cationic photosensitisers is a promising antimicrobial approach that avoids the development of resistance inherent to the use of antimicrobials. In this study, we demonstrate that modification of different photosensitisers with the triphenylphosphonium cation yields derivatives with excellent photoantimicrobial activity against Gram-positive bacteria (i.e., S. aureus and E. faecalis). Thus, the triphenylphosphonium functional group should be considered for the development of photoantimicrobials for the selective killing of Gram-positive bacteria in the presence of Gram-negative species...
June 8, 2018: Journal of Biophotonics
Sviatlana Kalinina, Jasmin Breymayer, Kirsten Reeß, Lothar Lilge, Arkady Mandel, Angelika Rück
During PDT disruption of cell respiration and metabolic changes could be one of the first events. Photophysical characteristics of the photosensitizer (PS) and its specific redox potential define consumption of molecular oxygen followed by generation of reactive oxygen species (ROS). The potential photosensitizer TLD1433 is based on transition metal Ru(II) and possess an oxygen dependent luminescence. This enables study of oxygen consumption by PS-PLIM (phosphorescence lifetime imaging) and simultaneously changes of the cellular metabolic state by NAD(P)H-FLIM (fluorescence lifetime imaging)...
June 7, 2018: Journal of Biophotonics
Itay Barnea, Lidor Karako, Simcha K Mirsky, Mattan Levi, Michal Balberg, Natan T Shaked
Acridine orange (AO) staining is one of the methods used to diagnose the DNA fragmentation status in sperm cells. Interferometric phase microscopy (IPM) is a an optical imaging method based on digital holographic microscopy that provides quantitative morphological and refractive index imaging of cells in vitro without the need for staining. We have imaged sperm cells using stain-free IPM in order to estimate different cellular parameters, such as acrosome dry mass and size, in addition to an embryologist evaluation according to the WHO-2010 criteria...
June 7, 2018: Journal of Biophotonics
Huda M Almabadi, Prashanth K B Nagesh, Peeyush Sahay, Shiva Bhandari, Eugene C Eckstein, Meena Jaggi, Subhash C Chauhan, Murali M Yallappu, Prabhakar Pradhan
As cancer progresses, macromolecules, such as DNA, RNA, or lipids, inside cells undergo spatial structural rearrangements and alterations. Mesoscopic light transport based optical partial wave spectroscopy (PWS) was recently introduced to quantify changes in the nanoscale structural disorder in biological cells. The PWS measurement is performed using a parameter termed as 'disorder strength' (Ld ), which represents the degree of nanoscale structural disorder inside the cells. It was shown that cancerous cells have higher disorder strength than normal cells...
June 5, 2018: Journal of Biophotonics
Weiye Song, Libo Zhou, Kevin Liu Kot, Huijie Fan, Jingyan Han, Ji Yi
Flow-mediated vasodilation (FMD) is used for assessment of vascular endothelial function in humans as a predictor of cardiovascular events. It has been challenging to carry it on preclinical murine models due to the diminutive size of the femoral artery. Here, we present a new approach to accurately measure the blood velocity and femoral artery diameters of mice by acquiring Doppler optical coherence tomography (DOCT) and optical coherence tomography angiography (OCTA) continuously within one single experimental scanning protocol...
May 31, 2018: Journal of Biophotonics
Matthias Domke, Sebastian Wick, Maike Laible, Stephan Rapp, Heinz P Huber, Ronald Sroka
Several studies on hard tissue laser ablation demonstrated that ultrafast lasers enable precise material removal without thermal side effects. Although the principle ablation mechanisms have been thoroughly investigated, there are still open questions regarding the influence of material properties on transient dynamics. In this investigation, we applied pump-probe microscopy to record ablation dynamics of biomaterials with different tensile strengths (dentin, chicken bone, gallstone, kidney stones) at delay times between 1 ps and 10 μs...
May 29, 2018: Journal of Biophotonics
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