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Journal of Biophotonics

Peeyush Sahay, Aditya Ganju, Huda M Almabadi, Hemendra M Ghimire, Murali M Yallapu, Omar Skalli, Meena Jaggi, Subhash C Chauhan, Prabhakar Pradhan
Light localization is a phenomenon which arises due to the interference effects of light waves inside a disordered optical medium. Quantification of degree light localization in optical media is widely used for characterizing degree of structural disorder in that media. Recently, this light localization approach was extended to analyze structural changes in biological cell like heterogeneous optical media, with potential application in cancer diagnostics. Confocal fluorescence microscopy was used to construct 'optical lattices', which represents 2D refractive index map corresponding to the spatial mass density distribution of a selected molecule inside the cell...
December 9, 2017: Journal of Biophotonics
Julie Chang, Gordon Wetzstein
Deep tissue imaging in the multiple scattering regime remains at the frontier of fluorescence microscopy. Speckle correlation imaging (SCI) can computationally uncover objects hidden behind a scattering layer, but has only been demonstrated with scattered laser illumination and in geometries where the scatterer is in the far field of the target object. Here SCI is extended to imaging a planar fluorescent signal at the back surface of a 500-μ m-thick slice of mouse brain. The object is reconstructed from a single snapshot via phase retrieval using a proximal algorithm that easily incorporates image priors...
December 8, 2017: Journal of Biophotonics
Hendrik Spahr, Dierck Hillmann, Clara Pfäffle, Gereon Hüttmann
In their latest publication on retinal pulse wave velocity measurement using optical co-herence tomography (OCT), Qian Li et al. propose a method to determine the pulse wave velocity in retinal arteries and veins [1]. This should enable a non-invasive determination of biomechanical properties of the vessel network, particularly the elasticity of the vessel walls [2].
December 8, 2017: Journal of Biophotonics
Damien Traynor, Padraig Kearney, Ines Ramos, Cara M Martin, John J O'Leary, Fiona M Lyng
Raman spectroscopy is a powerful tool that has the potential to be used as a screening method for cervical cancer. It is a label free, low cost method providing a biochemical fingerprint of a given sample. The objective of this study was to address patient to patient variability contributed by hormonal effects due to the menstrual cycle, the use of hormone based contraceptives and the onset of menopause, and to determine if these changes would affect the ability to successfully identify dyskaryotic cells. Raman spectra were recorded from unstained ThinPrep cervical samples (45 cytology negative and 15 high grade dyskaryosis (high grade squamous intraepithelial lesion (SIL)) samples using a HORIBA Jobin Yvon XploRA™ system...
December 7, 2017: Journal of Biophotonics
Geonho Choi, Jeonghyeon Lee, Hyeongeun Kim, Jaemyung Jang, Changkyun Im, Nooli Jeon, Woonggyu Jung
In this study, we introduce the novel image-guided recording system (IGRS) for efficient interpretation of neuronal activities in the brain slice. IGRS is designed to combine microelectrode array (MEA) and optical coherence tomography at the customized upright microscope. It allows to record multi-site neuronal signals and image the volumetric brain anatomy in a single body configuration. For convenient interconnection between a brain image and neuronal signals, we developed the automatic mapping protocol which enables for projecting acquired neuronal signals on a brain image...
December 7, 2017: Journal of Biophotonics
Jianlong Yang, Rahul Chandwani, Rui Zhao, Zhuo Wang, Yali Jia, David Huang, Gangjun Liu
A polarization-multiplexed, dual-beam setup is proposed to expand the field of view for a swept source optical coherence tomography angiography (OCTA) system. This method used a Wollaston prism to split sample path light into two orthogonal-polarized beams. This allowed two beams to shine on the cornea at an angle separation of ~ 14 degrees, which led to a separation of ~ 4.2 mm on the retina. A 3-mm glass plate was inserted into one of the beam paths to set a constant path length difference between the two polarized beams so the interferogram from the two beams are coded at different frequency bands...
December 6, 2017: Journal of Biophotonics
Jessica Miller, Steven T Wang, Inema Orukari, Julie Prior, Gail Sudlow, Xinming Su, Kexian Liang, Rui Tang, Elizabeth M C Hillman, Katherine N Weilbaecher, Joseph P Culver, Mikhail Y Berezin, Samuel Achilefu
Rapid detection of multifocal cancer without the use of complex imaging schemes will improve treatment outcomes. In this study, dynamic fluorescence imaging was used to harness differences in the perfusion kinetics of near infrared fluorescent dyes to visualize structural characteristics of different tissues. Using the hydrophobic non-tumor-selective near infrared dye cypate, and the hydrophilic dye LS288, a high tumor-to-background contrast was achieved, allowing the delineation of diverse tissue types while maintaining short imaging times...
December 5, 2017: Journal of Biophotonics
Yueyue Jing, Yulan Wang, Xinyi Wang, Chuan Song, Jiong Ma, Yonghui Xie, Yiyan Fei, Qinghua Zhang, Lan Mi
The label-free imaging and spectroscopy method was studied on cervical unstained tissue sections obtained from 36 patients. The native fluorescence spectra of tissues are analyzed by the optical redox ratio (ORR), which is defined as fluorescence intensity ratio between NADH and FAD, and indicates the metabolism change with the cancer development. The ORRs of normal tissues are consistently higher than those of precancer or cancerous tissues. A criterion line of ORR at 5.0 can be used to discriminate cervical precancer/cancer from normal tissues...
December 5, 2017: Journal of Biophotonics
Atsushi Tsukiyama, Yasuo Murai, Fumihiro Matano, Kazutaka Shirokane, Akio Morita
Various reports have been published regarding quantitative evaluations of intraoperative fluorescent intensity studies using indocyanine green (ICG) with videoangiography (VAG). The effects of scattering and point-spread functions (PSF) on quantitative ICG-VAG evaluations have not been investigated. Clinically, when ICG is administered through the peripheral vein, it reaches the tissue intra-arterially. To achieve more reliable intraoperative quantitative intensity evaluations, we examined the impact of high-intensity structures on close areas...
November 29, 2017: Journal of Biophotonics
Nadine Döge, Sabrina Hadam, Pierre Volz, Alexander Wolf, Karl-Heinz Schönborn, Ulrike Blume-Peytavi, Ulrike Alexiev, Annika Vogt
The question whether nanoparticles can cross the skin barrier is highly debated. Even in intact skin rare events of deeper penetration have been reported, but technical limitations and possible artefacts require careful interpretation. In this study, horizontal scanning by two-photon microscopy (2 PM) of full-thickness human skin samples placed in a lateral position yielded highly informative images for skin penetration studies of fluorescently tagged nanoparticles. Scanning of large fields of view allowed for detailed information on interfollicular and follicular penetration in tissue blocks without damaging the sample...
November 27, 2017: Journal of Biophotonics
Luiza Filipis, Karima Ait Ouares, Philippe Moreau, Dimitrii Tanese, Valeria Zampini, Andrea Latini, Chun Bleau, Charlie Bleau, Jeremy Graham, Marco Canepari
In brain slices, resolving fast Ca(2+) fluorescence signals from submicron structures is typically achieved using two-photon or confocal scanning microscopy, an approach that limits the number of scanned points. The novel multiplexing confocal system presented here overcomes this limitation. This system is based on a fast spinning disk, a multimode diode laser and a novel high-resolution CMOS camera. The spinning disk, running at 20,000 rpm, has custom-designed spiral pattern that maximises light collection while rejecting out-of-focus fluorescence to resolve signals from small neuronal compartments...
November 22, 2017: Journal of Biophotonics
Mika Ishigaki, Takashi Nishii, Paralee Puangchit, Yui Yasui, Christian W Huck, Yukihiro Ozaki
In the present study, the distribution of biomaterials and its molecular mechanism of embryonic development in Japanese medaka fish were analyzed nondestructively and noninvasively without staining using near-infrared (NIR) imaging. The microscope NIR imaging system used in this research was a device capable of ultra-high-speed imaging; using this system, one can acquire microscopic imaging data in a few seconds. Therefore, the medaka eggs remained alive throughout measurements and were successfully monitored in vivo...
November 22, 2017: Journal of Biophotonics
Xinwen Yao, Yu Gan, Yuye Ling, Charles C Marboe, Christine P Hendon
A single-channel high-resolution cross-polarization (CP) optical coherence tomography (OCT) system is presented for multi-contrast imaging of human myocardium in one-shot measurement. The intensity contrast and functional contrasts, including the ratio between the cross- and co- polarization channels as well as the cumulative retardation, are reconstructed from the CP-OCT readout. By comparing the CP-OCT results with histological analysis, it is shown that the system can successfully delineate micro-structures in the myocardium and differentiate the fibrotic myocardium from normal or ablated myocardium based the functional contrasts provided by the CP-OCT system...
November 22, 2017: Journal of Biophotonics
Tanmoy T Bhattacharjee, Mariana C Nicodemo, Luciana B Sant'Anna, Emilia A Lo Schiavo Arisawa, Leandro Raniero
Tendinopathy, an important sports injury afflicting athletes and general public, is associated with huge economic losses. The currently used diagnostic tests are subjective, show moderate sensitivity and specificity; while treatment failures persist despite advances in therapy. This highlights the need for tendinopathy diagnostic and treatment monitoring tools. The current study investigates tendon injury, natural healing and effect of treatment using ATR-FTIR complemented with histopathology. Control (C), injured (I) and treated (T) rat tendons were extracted 3, 7, 14 and 28 days post injury/treatment, representing phases of healing; and subjected to H&E staining as well as spectroscopy...
November 21, 2017: Journal of Biophotonics
Mohamed T El-Haddad, Ivan Bozic, Yuankai K Tao
Non-invasive biological imaging is crucial for understanding in vivo structure and function. Optical coherence tomography (OCT) and reflectance confocal microscopy are two of the most widely used optical modalities for exogenous contrast-free high-resolution three-dimensional imaging in non-fluorescent scattering tissues. However, sample motion remains a critical barrier to raster-scanned acquisition and reconstruction of wide-field anatomically accurate volumetric datasets. We introduce spectrally encoded coherence tomography and reflectometry (SECTR), a high-speed multimodality system for simultaneous OCT and spectrally-encoded reflectance (SER) imaging...
November 17, 2017: Journal of Biophotonics
Stefano Managò, Nunzia Migliaccio, Monica Terracciano, Michela Napolitano, Nicola M Martucci, Luca De Stefano, Ivo Rendina, Anna Chiara De Luca, Annalisa Lamberti, Ilaria Rea
Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non-toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution of functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up to 72 h are investigated by Raman imaging. The label-free Raman results are compared with confocal fluorescence microscopy and photoluminescence (PL) data. Raman bands specifically assigned to DNPs and cellular components provide evidence that the nanovectors are internalized and co-localize with lipid environments...
November 16, 2017: Journal of Biophotonics
Lynda Miloudi, Franck Bonnier, Ali Tfayli, Florent Yvergnaux, Hugh J Byrne, Igor Chourpa, Emilie Munnier
Topically applied Active Cosmetic Ingredients (ACI) or Active Pharmaceutical Ingredients (API) efficacy is directly related to their efficiency of penetration in the skin. In vitro Reconstructed Human Epidermis (RHE) surrogate models offer in vivo like skin samples for transdermal studies. Using Delipidol®, an ACI currently used in the cosmetics industry, the capabilities to deliver accurate distribution maps and penetration profiles of this molecule by means of Confocal Raman spectroscopic Imaging have been demonstrated...
November 16, 2017: Journal of Biophotonics
Ji Qi, Honghui He, Jianyu Lin, Yang Dong, Dongsheng Chen, Hui Ma, Daniel S Elson
Tissue depolarization and linear retardance are the main polarization characteristics of interest for bulk tissue characterization, and are normally interpreted from Mueller polarimetry. Stokes polarimetry can be conducted using simpler instrumentation and in a shorter time. Here we use Stokes polarimetric imaging with circularly polarized illumination to assess the circular depolarization and linear retardance properties of tissue. Results obtained were compared with Mueller polarimetry in transmission and reflection geometry respectively...
November 13, 2017: Journal of Biophotonics
Hamootal Duadi, Idit Feder, Dror Fixler
Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have proposed examining the full scattering profile (FSP), which is the angular intensity distribution. A point was found, i.e. the iso-pathlength (IPL) point, which is not dependent on the tissue scattering, and can serve for self-calibration...
November 13, 2017: Journal of Biophotonics
Jinchao Feng, Kebin Jia, Zhe Li, Brian W Pogue, Mingjie Yang, Yaqi Wang
Bioluminescence tomography (BLT) provides fundamental insight into biological processes in vivo. To fully realize its potential, it is important to develop image reconstruction algorithms that accurately visualize and quantify the bioluminescence signals taking advantage of limited boundary measurements. In this study, a new two-step reconstruction method for BLT is developed by taking advantage of the sparse a priori information of the light emission using multispectral measurements. The first step infers a wavelength-dependent prior by using all multi-wavelength measurements...
November 9, 2017: Journal of Biophotonics
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