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Journal of Biophotonics

Ang Li, Gunnsteinn Hall, Defu Chen, Wenxuan Liang, Bo Ning, Honghua Guan, Xingde Li
In this work, we report a biopsy-needle compatible rigid probe, capable of performing 3-dimensional two-photon optical biopsy. The probe has a small outer diameter of 1.75 mm and fits inside a gauge-14 biopsy needle to reach internal organs. A carefully designed focus scanning mechanism has been implemented in the rigid probe, which, along with a rapid 2D MEMS scanner, enables 3D imaging. Fast image acquisition up to 10 frames per second is possible, dramatically reducing motion artifacts during in vivo imaging...
August 16, 2018: Journal of Biophotonics
Nhan M Le, Shaozhen Song, Hao Zhou, Jingjiang Xu, Yuandong Li, Cheng-En Sung, Alireza Sadr, Kwok-Hung Chung, Hrebesh M Subhash, Latonya Kilpatrick, Ruikang K Wang
Gingiva is the soft tissue that surrounds and protects the teeth. Healthy gingiva provides an effective barrier to periodontal insults to deeper tissue, thus is an important indicator to a patient's periodontal health. Current methods in assessing gingival tissue health, including visual observation and physical examination with probing on the gingiva, are qualitative and subjective. They may become cumbersome when more complex cases are involved, such as variations in gingival biotypes where feature and thickness of the gingiva are considered...
August 15, 2018: Journal of Biophotonics
Sandeep Chakraborty, Szu-Yu Lee, Jye-Chang Lee, Chen-Tung Yen, Chi-Kuang Sun
Optical imaging is a key modality for observing biological specimen with higher spatial resolution. However, scattering and absorption of light in tissues are inherent barriers in maximizing imaging depth in biological tissues. To achieve this goal, use of light at near infrared (NIR) spectrum can improve the present situation. Here, the capability of saturated two-photon excitation fluorescence microscopy (TP-SAX) to image at depths of > 2.0 mm, with submicron resolution in transparent mouse brain imaging, is demonstrated...
August 15, 2018: Journal of Biophotonics
Shamira Sridharan, Yanfen Li, Louis Foucard, Hassaan Majeed, Basanta Bhaduri, Alex Levine, Kristopher Kilian, Gabriel Popescu
Characterizing the effects of force fields generated by cells on proliferation, migration, and differentiation processes is challenging due to limited availability of non-destructive imaging modalities. Here, we integrate a new real-time traction stress imaging modality, Hilbert phase dynamometry (HPD), with spatial light interference microscopy (SLIM) for simultaneous monitoring of cell growth during differentiation processes. HPD uses holographic principles to extract displacement fields from chemically patterned fluorescent grid on deformable substrates...
August 14, 2018: Journal of Biophotonics
Leonie Ulrich, Linda Ahnen, H Günhan Akarçay, Salvator Sanchez, Michael Jaeger, Gerrit Held, Martin Wolf, Martin Frenz
In vivo imaging of tissue/vasculature oxygen saturation levels is of prime interest in many clinical applications. To this end, the feasibility of combining two distinct and complementary imaging modalities is investigated: optoacoustics (OA) and near-infrared optical tomography (NIROT), both operating non-invasively in reflection mode. Experiments were conducted on two optically heterogeneous phantoms mimicking tissue before and after the occurrence of a perturbation. OA imaging was used to resolve submillimetric vessel-like optical absorbers at depths up to 25 mm, but with a spectral distortion in the OA signals...
August 11, 2018: Journal of Biophotonics
Sergii Golovynskyi, Iuliia Golovynska, Ludmila I Stepanova, Oleksandr I Datsenko, Liwei Liu, Junle Qu, Tymish Y Ohulchanskyy
Optical properties of the rat head tissues (brain cortex, cranial bone and scalp skin) are assessed, aiming at transcranial light applications such as optical imaging and phototherapy. The spectral measurements are carried out over the wide spectral range of 350-2800 nm, involving visible, near-infrared (NIR) and short-wave infrared (SWIR) regions. Four tissue transparency windows are considered: ~700-1000 nm (NIR-I), ~1000-1350 nm (NIR-II), ~1550-1870 nm (NIR-III or SWIR) and ~2100-2300 nm (SWIR-II). The values of attenuation coefficient and total attenuation length are determined for all windows and tissue types...
August 11, 2018: Journal of Biophotonics
Fernanda Rossi Paolillo, Vicente Silva Mattos, André Orlandi de Oliveira, Francisco Eduardo Gontijo Guimarães, Vanderlei Salvador Bagnato, Jarbas Caiado de Castro Neto
Diabetes is a complex metabolic disease and has chronic complications. It has been considered a serious public health problem. The aim of the current study was to evaluate skin glycated proteins through fluorescence and Raman techniques. One hundred subjects were invited to participate in the study. Six volunteers did not attend due to exclusion criteria or a change of mind about participating. Therefore, ninety four volunteers were grouped according to age range (20 to 80 years), health condition (non-diabetic, with insulin resistance (IR) and/or diabetic) and Fitzpatrick skin type (I to VI)...
August 9, 2018: Journal of Biophotonics
H Hajjar, H Boukhaddaoui, A Rizgui, C Sar, J Berthelot, C Perrin-Tricaud, H Rigneault, N Tricaud
Myelin sheath produced by Schwann cells covers and nurtures axons to speed up nerve conduction in peripheral nerves. Demyelinating peripheral neuropathies result from the loss of this myelin sheath and so far, no treatment exists to prevent Schwann cell demyelination. One major hurdle to design a therapy for demyelination is the lack of reliable measures to evaluate the outcome of the treatment on peripheral myelin in patients but also in living animal models. Non-linear microscopy techniques SHG, THG and CARS were used to image myelin ex vivo and in vivo in the sciatic nerve of healthy and demyelinating mice and rats...
August 9, 2018: Journal of Biophotonics
Byullee Park, Hoyong Lee, Seungwan Jeon, Joongho Ahn, Hyung Ham Kim, Chulhong Kim
We have developed a reflection-mode switchable subwavelength Bessel-beam (BB) and Gaussian-beam (GB) photoacoustic microscopy (PAM) system. To achieve both reflection-mode and high-resolution, we tightly attached a very small ultrasound transducer to an optical objective lens with numerical aperture of 1.0 and working distance of 2.5mm. We used axicon and an achromatic doublet in our system to obtain the extended depth of field (DOF) of the BB. To compare the DOF performance achieved with our Bessel-beam PAM (BB-PAM) system against Gaussian-beam PAM (GB-PAM) system, we designed our system so that the GB can be easily generated by simply removing the lenses...
August 6, 2018: Journal of Biophotonics
Martina Mugnano, Pasquale Memmolo, Lisa Miccio, Simonetta Grilli, Francesco Merola, Alejandro Calabuig, Alessia Bramanti, Emanuela Mazzon, Pietro Ferraro
Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH) based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects...
August 5, 2018: Journal of Biophotonics
Martin Köhler, Bjorn Paulson, Youngkyu Kim, Sanghwa Lee, Andrea Dicker, Pim van Krieken, Jae Young Kim, Chan-Gi Pack, Jinmyoung Joo, Per-Olof Berggren, Jun Ki Kim
Intravital optical imaging technology is essential for minimally invasive optical diagnosis and treatment in small animal disease models. High resolution imaging requires high resolution optical probes, and high resolution optical imaging systems based on highly precise and advanced technologies and therefore associated with high system costs. Besides, in order to acquire small animal live images, special types of animal imaging setups are indispensable. In this paper, a micro-endoscopic system is designed as an add-on to existing conventional imaging microscopes, reducing the price of complete confocal endomicroscopic systems...
August 5, 2018: Journal of Biophotonics
Hanna C McGregor, Michael A Short, Stephen Lam, Tawimas Shaipanich, Eve-Léa Beaudoin, Haishan Zeng
The management of cancer in the periphery lung is in critical need of new strategies. Here the development and test of a novel miniature Raman probe capable of navigating the peripheral lung architecture is reported. The probe was 1.35 mm in diameter, with a minimum bend radius of 13 mm and had a large light collection area for its size. Peripheral lung Raman spectra were successfully obtained from normal tissue and cancerous nodule using the probe coupled to a home-made rapid Raman spectroscopy system with a fast integration time of 1 s and a low excitation power of 15 mW...
August 5, 2018: Journal of Biophotonics
Kanheng Zhou, Chunhui Li, Siping Chen, Ghulam Nabi, Zhihong Huang
The aim of this study was to investigate the feasibility of utilizing the phase velocity dispersion of impulse surface acoustic wave (SAW) for viscoelasticity characterization of soft materials. The focused ultrasound transducer and the phase sensitive optical coherence tomography (PhS-OCT) were applied as the impulse SAW inducer and tracker respectively. Three types of liquid-paraffin-based cream-in-agar phantoms were tested. Phase velocity dispersion curve was extracted using a Fourier transform based phase velocity analysis algorithm...
August 3, 2018: Journal of Biophotonics
Jonas Ogien, Arnaud Dubois
A compact high-speed full-field optical coherence microscope has been developed for high-resolution in vivo imaging of biological tissues. The interferometer, in the Linnik configuration, has a size of 11×11×5 cm3 and a weight of 210 g. Full-field illumination with low-coherence light is achieved with a high-brightness broadband light-emitting diode. High-speed full-field detection is achieved by using part of the image sensor of a high-dynamic range CMOS camera. En face tomographic images are acquired at a rate of 50 Hz, with an integration time of 0...
July 30, 2018: Journal of Biophotonics
Rajinder P Singh-Moon, Xinwen Yao, Vivek Iyer, Charles Marboe, William Whang, Christine P Hendon
Despite considerable advances in guidance of radiofrequency ablation (RFA) therapy for the treatment of cardiac arrhythmias, success rates have been hampered by a lack of tools for precise intraoperative evaluation of lesion extent. Near-infrared spectroscopic (NIRS) techniques are sensitive to tissue structural and biomolecular properties, characteristics that are directly altered by RF treatment. In this work, a combined NIRS-RFA catheter is developed for real-time monitoring of tissue reflectance during RF energy delivery...
July 30, 2018: Journal of Biophotonics
Edorta Santos-Vizcaino, Henry Haley, Ainhoa Gonzalez-Pujana, Gorka Orive, Rosa Maria Hernandez, Gary D Luker, Jose Luis Pedraz
Imaging of implanted hydrogel-based biosystems usually requires indirect labelling of the vehicle or cargo, adding complexity and potential risk of altering functionality. Here, for the first time, it is reported that incorporating genipin into the design of immunoisolation devices can be harnessed for in vivo imaging. Using cell-compatible in situ cross-linking reactions, a fast, efficient and non-cytotoxic procedure is shown to maximize fluorescence of microcapsules. Moreover, genipin is validated as a quantitative imaging probe by injecting increasing doses of microcapsules in the subcutaneous space of mice, obtaining strong, stable fluorescence with good linearity of signal to microcapsule dose over several weeks...
July 29, 2018: Journal of Biophotonics
Wes M Allen, Philip Wijesinghe, Benjamin F Dessauvagie, Bruce Latham, Christobel M Saunders, Brendan F Kennedy
Accurate and effective removal of tumor in one operation is an important goal of breast-conserving surgery. However, it is not always achieved. Surgeons often utilize manual palpation to assess the surgical margin and/or the breast cavity. Manual palpation, however, is subjective and has relatively low-resolution. Here, we investigate a tactile imaging technique, optical palpation, for the visualization of tumor. Optical palpation generates maps of the stress at the surface of tissue under static preload compression...
July 28, 2018: Journal of Biophotonics
Hao Jia, Xianghua Yu, Yanlong Yang, Xing Zhou, Shaohui Yan, Chao Liu, Ming Lei, Baoli Yao
The side lobes of Bessel beam will create significant out-of-focus background when scanned in light-sheet fluorescence microscopy (LSFM), limiting the axial resolution of the imaging system. Here, we propose to overcome this issue by scanning the sample twice with zeroth-order Bessel beam and another type of propagation-invariant beam, complementary to the zeroth-order Bessel beam, which greatly reduces the out-of-focus background created in the first scan. The axial resolution can be improved from 1.68μm of the Bessel light-sheet to 1...
July 25, 2018: Journal of Biophotonics
Pengbo Wang, Ting Li
One of the challenges in transcranial low-level laser therapy (LLLT) is to optimally choose illumination parameters, such as wavelength. However, there is sparse study on the wavelengths comparison especially on human transcranial LLLT. Here, we employed Monte Carlo modeling and visible human phantom to compute the penetrated photon fluence distribution within cerebral cortex. By comparing the fluence distribution, penetration depth, and the intensity of laser-tissue-interaction within brain among all candidate wavelengths, we found that 660, 810 nm performed much better than 980, 1064 nm with much stronger, deeper, and wider photon penetration into cerebral tissue...
July 25, 2018: Journal of Biophotonics
Yulia M Alexandrovskaya, Evgeniy G Evtushenko, Mariya M Obrezkova, Valery V Tuchin, Emil N Sobol
Infrared (IR) laser impact has no analogues for rapid and safe cartilage reshaping. For better penetration of radiation optical clearing agents (OCAs) can be applied. In present work the effect of low-osmolality agent iohexol on costal cartilage is studied. Specifically, it is shown that ½ of total increase of optical transparency occurs in 20 min of immersion. Maximally, cartilage transparency on 1560 nm can be increased in 1.5 times. Injection of iohexol results in increased tissue hygroscopicity, lower drying rate and higher percentage of bound water...
July 25, 2018: Journal of Biophotonics
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