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Journal of Biophotonics

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https://www.readbyqxmd.com/read/28635183/cetuximab-conjugated-nanodiamonds-drug-delivery-system-for-enhanced-targeting-therapy-and-3d-raman-imaging
#1
Dandan Li, Xin Chen, Hong Wang, Jie Liu, Meiling Zheng, Yang Fu, Yuan Yu, Jinfang Zhi
In this study, a multicomponent nanodiamonds (NDs)-based targeting drug delivery system, cetuximab-NDs-cisplatin bioconjugate, combining both specific targeting and enhanced therapeutic efficacy capabilities, is developed and characterized. The specific targeting ability of cetuximab-NDs-cisplatin system on human liver hepatocellular carcinoma (HepG2) cells is evaluated through epidermal growth factor receptor (EGFR) blocking experiments, since EGFR is over-expressed on HepG2 cell membrane. Besides, cytotoxic evaluation confirms that cetuximab-NDs-cisplatin system could significantly inhibit the growth of HepG2 cells, and the therapeutic activity of this system is proven to be better than that of both nonspecific NDs-cisplatin conjugate and specific EGF-NDs-cisplatin conjugate...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635172/doxorubicin-kinetics-and-effects-on-lung-cancer-cell-lines-using-in-vitro-raman-micro-spectroscopy-binding-signatures-drug-resistance-and-dna-repair
#2
Zeineb Farhane, Franck Bonnier, Orla Howe, Alan Casey, Hugh J Byrne
Raman micro-spectroscopy is a non-invasive analytical tool, whose potential in cellular analysis and monitoring drug mechanisms of action has already been demonstrated, and which can potentially be used in pre-clinical and clinical applications for the prediction of chemotherapeutic efficacy. To further investigate such potential clinical application, it is important to demonstrate its capability to differentiate drug mechanisms of action and cellular resistances. Using the example of Doxorubicin (DOX), in this study, it was used to probe the cellular uptake, signatures of chemical binding and subsequent cellular responses, of the chemotherapeutic drug in two lung cancer cell lines, A549 and Calu-1...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635151/propagation-of-gaussian-and-laguerre-gaussian-vortex-beams-through-mouse-brain-tissue
#3
Lingyan Shi, Lukas Lindwasser, Wubao Wang, Robert Alfano, Adrián Rodríguez-Contreras
Light transmission of Gaussian (G) and Laguerre-Gaussian (LG) vortex beams in mouse brain tissue is investigated. Transmittance is measured with different orbital angular momentums (OAM) at various tissue thicknesses. In both ballistic and diffusive regions, transmittances of G and LG beams show no significant difference. The transition point from ballistic to diffusive region for the mouse brain tissue is determined at about 480 μm. The observed transmittances of the G and LG beams show independence on OAM modes, which may be attributed to poorly understood interference effects from brain tissue...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635141/high-sensitivity-non-invasive-detection-of-calcifications-deep-inside-biological-tissue-using-transmission-raman-spectroscopy
#4
Adrian Ghita, Pavel Matousek, Nick Stone
The aim of this research was to develop a novel approach to probe non-invasively the composition of inorganic chemicals buried deep in large volume biological samples. The method is based on advanced Transmission Raman Spectroscopy (TRS) permitting chemical specific detection within a large sampling volume. The approach could be beneficial to chemical identification of the breast calcifications detected during mammographic X-ray procedures. The chemical composition of a breast calcification reflects the pathology of the surrounding tissue, malignant or benign and potentially the grade of malignancy...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635128/development-of-a-classification-model-for-non-alcoholic-steatohepatitis-nash-using-confocal-raman-micro-spectroscopy
#5
Jie Yan, Yang Yu, Jeon Woong Kang, Zhi Yang Tam, Shuoyu Xu, Eliza Li Shan Fong, Surya Pratap Singh, Ziwei Song, Lisa Tucker-Kellogg, Peter T C So, Hanry Yu
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in developed countries [1]. A subset of individuals with NAFLD progress to non-alcoholic steatohepatitis (NASH), an advanced form of NAFLD which predisposes individuals to cirrhosis, liver failure and hepatocellular carcinoma. The current gold standard for NASH diagnosis and staging is based on histological evaluation, which is largely semi-quantitative and subjective. To address the need for an automated and objective approach to NASH detection, we combined Raman micro-spectroscopy and machine learning techniques to develop a classification model based on a well-established NASH mouse model, using spectrum pre-processing, biochemical component analysis (BCA) and logistic regression...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635120/diffractive-corneal-inlay-for-presbyopia
#6
LETTER
Walter D Furlan, Salvador García-Delpech, Patricia Udaondo, Laura Remón, Vicente Ferrando, Juan A Monsoriu
A conceptually new type of corneal inlays for a customized treatment of presbyopia is presented. The diffractive inlay consists on a small aperture disc having an array of micro-holes distributed inside the open zones of a Fresnel zone plate. In this way, the central hole of the disc lets pass the zero order diffraction and produces an extension of the depth of far focus of the eye, while the diffracted light through the holes in the periphery produce the near focus. Additionally, the micro-holes in the inlay surface fulfill the essential requirement of allowing the flow of nutrients through it to the cells of the corneal stroma...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28635102/ratiometric-analysis-of-optical-coherence-tomography-measured-in%C3%A2-vivo-retinal-layer-thicknesses-for-the-detection-of-early-diabetic-retinopathy
#7
Basanta Bhaduri, Ryan L Shelton, Ryan M Nolan, Lucas Hendren, Alexandra Almasov, Leanne T Labriola, Stephen A Boppart
Influence of diabetes mellitus (DM) and diabetic retinopathy (DR) on parafoveal retinal thicknesses and their ratios was evaluated. Six retinal layer boundaries were segmented from spectral-domain optical coherence tomography images using open-source software. Five study groups: (1) healthy control (HC) subjects, and subjects with (2) controlled DM, (3) uncontrolled DM, (4) controlled DR and (5) uncontrolled DR, were identified. The one-way analyses of variance (ANOVA) between adjacent study groups (i. e...
June 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28544745/fs-laser-ablation-of-teeth-is-temperature-limited-and-provides-information-about-the-ablated-components
#8
Rebeca Ferraz de Menezes, Catherine Malinda Harvey, Marleny Elizabeth Márquez de Martínez Gerbi, Zachary J Smith, Dan Smith, Juan C Ivaldi, Alton Phillips, James W Chan, Sebastian Wachsmann-Hogiu
The goal of this work is to investigate the thermal effects of femtosecond laser (fs-laser) ablation for the removal of carious dental tissue. Additional studies identify different tooth tissues through femtosecond laser induced breakdown spectroscopy (fsLIBS) for the development of a feedback loop that could be utilized during ablation in a clinical setting. Scanning Election Microscope (SEM) images reveal that minimal morphological damages are incurred at repetition rates below the carbonization threshold of each tooth tissue...
May 24, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28544641/reflectance-spectroscopy-to-shed-new-light-on-the-capillary-refill-test
#9
Rani Toll John, Joakim Henricson, Gert E Nilsson, Daniel Wilhelms, Chris D Anderson
To use Bioengineering methodology is used to achieve, at five anatomical sites, a detailed, quantitative assessment of the return of blood content to the blanched area, during the Capillary Refill (CR) test. An observational, non-randomized, experimental study on 23 healthy subjects (14 females) was performed in our climate controlled skin physiology laboratory. Our main outcome measures were based on the chronological assessment and quantification of red blood cell concentration (RBC) after the release of blanching pressure in the CR test, using Tissue Viability Imaging (TiVi), a digital photographic technique based on polarisation spectroscopy...
May 24, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516738/intrinsic-fluorescence-and-mechanical-testing-of-articular-cartilage-in-human-patients-with-osteoarthritis
#10
Juan Pablo Padilla-Martinez, William Lewis, Antonio Ortega-Martinez, Walfre Franco
The degeneration of articular cartilage is the main cause of osteoarthritis (OA), a common cause of disability among elderly patients. The aim of this study is to understand the correlation between intrinsic fluorescence of articular cartilage and its biomechanical properties in patients with osteoarthritis. Cylindrical samples of articular cartilage 6 mm in diameter were extracted via biopsy punch from the femoral condyles of 6 patients with advanced OA undergoing knee replacement surgery. The mechanical stiffness and fluorescence of each cartilage plug were measured by indentation test and spectrofluorometry...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516672/two-photon-imaging-of-fiber-coupled-neurons
#11
M S Pochechuev, I V Fedotov, O I Ivashkina, M A Roshchina, K V Anokhin, A M Zheltikov
Optical coupling between a single, individually addressable neuron and a properly designed optical fiber is demonstrated. Two-photon imaging is shown to enable a quantitative in situ analysis of such fiber-single-neuron coupling in the live brain of transgenic mice. Fiber-optic interrogation of single pyramidal neurons in mouse brain cortex is performed with the positioning of the fiber probe relative to the neuron accurately mapped by means of two-photon imaging. These results pave the way for fiber-optic interfaces to single neurons for a stimulation and interrogation of individually addressable brain cells in chronic in vivo studies on freely behaving transgenic animal models, as well as the integration of fiber-optic single-neuron stimulation into the optical imaging framework...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516571/fsoca-induced-switchable-footpad-skin-optical-clearing-window-for-blood-flow-and-cell-imaging-in%C3%A2-vivo
#12
Rui Shi, Wei Feng, Chao Zhang, Zhihong Zhang, Dan Zhu
The mouse footpad for its feature of hairlessness provides an available window for imaging vascular and cellular structure and function in vivo. Unfortunately, the strong scattering of its skin limits the penetration of light and reduces the imaging contrast and depth. Herein, an innovative footpad skin optical clearing agent (FSOCA) was developed to make the footpad skin transparent quickly by topical application. The results demonstrate that FSOCA treatment not only allowed the cutaneous blood vessels and blood flow distribution to be monitored by laser speckle contrast imaging technique with higher contrast, but also permitted the fluorescent cells to be imaged by laser scanning confocal microscopy with higher fluorescence signal intensity and larger imaging depth...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516508/near-infrared-photodynamic-inactivation-of-s-pneumoniae-and-its-interaction-with-raw-264-7-macrophages
#13
Ilaiáli S Leite, Mariana C Geralde, Ana C G Salina, Alexandra I Medeiros, Lívia N Dovigo, Vanderlei S Bagnato, Natalia M Inada
Pneumonia is the main cause of children mortality worldwide, and its major treatment obstacle stems from the microorganisms increasing development of resistance to several antibiotics. Photodynamic therapy has been presenting, for the last decades, promising results for some subtypes of cancer and infections. In this work we aimed to develop a safe and efficient in vitro protocol for photodynamic inactivation of Streptococcus pneumoniae, one of the most commonly found bacteria in pneumonia cases, using two near-infrared light sources and indocyanine green, a FDA approved dye...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28516475/evaluation-of-detection-distance-dependent-reflectance-spectroscopy-for-the-determination-of-the-sun-protection-factor-using-pig-ear-skin
#14
Carina Reble, Ingo Gersonde, Sabine Schanzer, Martina C Meinke, Jürgen Helfmann, Jürgen Lademann
Determination of sun protection factors (SPFs) is currently an invasive method, which is based on erythema formation (phototest). Here we describe an optical setup and measurement methodology for the determination of SPFs based on diffuse reflectance spectroscopy, which measures UV-reflectance spectra at 4 distances from the point of illumination. Due to a high spatial variation of the reflectance data, most likely due to inhomogeneities of the sunscreen distribution, data of 50 measurement positions are averaged...
May 18, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28493437/extended-axial-imaging-range-widefield-swept-source-optical-coherence-tomography-angiography
#15
Gangjun Liu, Jianlong Yang, Jie Wang, Yan Li, Pengxiao Zang, Yali Jia, David Huang
We developed a high-speed, swept source OCT system for widefield OCT angiography (OCTA) imaging. The system has an extended axial imaging range of 6.6 mm. An electrical lens is used for fast, automatic focusing. The recently developed split-spectrum amplitude and phase-gradient angiography allow high-resolution OCTA imaging with only two B-scan repetitions. An improved post-processing algorithm effectively removed trigger jitter artifacts and reduced noise in the flow signal. We demonstrated high contrast 3 mm×3 mm OCTA image with 400×400 pixels acquired in 3 seconds and high-definition 8 mm×6 mm and 12 mm×6 mm OCTA images with 850×400 pixels obtained in 4 seconds...
May 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28493426/optical-coherence-elastography-for-strain-dynamics-measurements-in-laser-correction-of-cornea-shape
#16
Vladimir Y Zaitsev, Alexander L Matveyev, Lev A Matveev, Grigory V Gelikonov, Alexander I Omelchenko, Olga I Baum, Sergey E Avetisov, Andrey V Bolshunov, Vladimir I Siplivy, Dmitry V Shabanov, Alex Vitkin, Emil N Sobol
We describe the use of elastographic processing in phase-sensitive optical coherence tomography (OCT) for visualizing dynamics of strain and tissue-shape changes during laser-induced photothermal corneal reshaping, for applications in the emerging field of non-destructive and non-ablative (non-LASIK) laser vision correction. The proposed phase-processing approach based on fairly sparse data acquisition enabled rapid data processing and near-real-time visualization of dynamic strains. The approach avoids conventional phase unwrapping, yet allows for mapping strains even for significantly supra-wavelength inter-frame displacements of scatterers accompanied by multiple phase-wrapping...
May 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28488393/in-vitro-analysis-of-early-calcification-in-aortic-valvular-interstitial-cells-using-laser-induced-breakdown-spectroscopy-libs
#17
Seyyed Ali Davari, Shirin Masjedi, Zannatul Ferdous, Dibyendu Mukherjee
Calcific aortic valve disease (CAVD) is a major cardiovascular disorder caused by osteogenic differentiation of valvular interstitial cells (VICs) within aortic valves. Conventional methods like colorimetric assays and histology fail to detect small calcium depositions during in-vitro VIC cultures. Laser-induced breakdown spectroscopy (LIBS) is a robust analytical tool used for inorganic materials characterizations, but relatively new to biomedical applications. We employ LIBS, for the first time, for quantitative in-vitro detection of calcium depositions in VICs at various osteogenic differentiation stages...
May 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28485498/plasmonic-nanoparticles-decorated-diatomite-biosilica-extending-the-horizon-of-on-chip-chromatography-and-label-free-biosensing
#18
Xianming Kong, Erwen Li, Kenny Squire, Ye Liu, Bo Wu, Li-Jing Cheng, Alan X Wang
Diatomite consists of fossilized remains of ancient diatoms and is a type of naturally abundant photonic crystal biosilica with multiple unique physical and chemical functionalities. In this paper, we explored the fluidic properties of diatomite as the matrix for on-chip chromatography and, simultaneously, the photonic crystal effects to enhance the plasmonic resonances of metallic nanoparticles for surface-enhanced Raman scattering (SERS) biosensing. The plasmonic nanoparticle-decorated diatomite biosilica provides a lab-on-a-chip capability to separate and detect small molecules from mixture samples with ultra-high detection sensitivity down to 1 ppm...
May 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28485132/robust-numerical-phase-stabilization-for-long-range-swept-source-optical-coherence-tomography
#19
Shaozhen Song, Jingjiang Xu, Shaojie Men, Tueng T Shen, Ruikang K Wang
A novel phase stabilization technique is demonstrated with significant improvement in the phase stability of a micro-electromechanical (MEMS) vertical cavity surface-emitting laser (VCSEL) based swept-source optical coherence tomography (SS-OCT) system. Without any requirements of hardware modifications, the new fully numerical phase stabilization technique features high tolerance to acquisition jitter, and significantly reduced budget in computational effort. We demonstrate that when measured with biological tissue, this technique enables a phase sensitivity of 89 mrad in highly scattering tissue, with image ranging distance of up to 12...
May 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28485130/fluorescence-hyperspectral-imaging-fhsi-using-a-spectrally-resolved-detector-array
#20
Anna Siri Luthman, Sebastian Dumitru, Isabel Quiros-Gonzalez, James Joseph, Sarah E Bohndiek
The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral information in a single data acquisition, however, instrumentation for HSI is typically complex, bulky and expensive. We sought to overcome these limitations using a novel robust and low cost HSI camera based on a spectrally resolved detector array (SRDA)...
May 9, 2017: Journal of Biophotonics
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