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Journal of Biophotonics

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https://www.readbyqxmd.com/read/28164480/real-time-raman-and-srs-imaging-of-living-human-macrophages-reveals-cell-to-cell-heterogeneity-and-dynamics-of-lipid-uptake
#1
Clara Stiebing, Tobias Meyer, Ingo Rimke, Christian Matthäus, Michael Schmitt, Stefan Lorkowski, Jürgen Popp
Monitoring living cells in real-time is important in order to unravel complex dynamic processes in life sciences. In particular the dynamics of initiation and progression of degenerative diseases is intensely studied. In atherosclerosis the thickening of arterial walls is related to high lipid levels in the blood stream, which trigger the lipid uptake and formation of droplets as neutral lipid reservoirs in macrophages in the arterial wall. Unregulated lipid uptake finally results in foam cell formation, which is a hallmark of atherosclerosis...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28164461/flexible-polygon-mirror-based-laser-scanning-microscope-platform-for-multiphoton-in-vivo-imaging
#2
Y X Li, V Gautam, A Brüstle, I A Cockburn, V R Daria, C Gillespie, K Gaus, C Alt, W M Lee
Commercial microscopy systems make use of tandem scanning i.e. either slow or fast scanning. We constructed, for the first time, an advanced control system capable of delivering a dynamic line scanning speed ranging from 2.7 kHz to 27 kHz and achieve variable frame rates from 5 Hz to 50 Hz (512 × 512). The dynamic scanning ability is digitally controlled by a new customized open-source software named PScan1.0. This permits manipulation of scanning rates either to gain higher fluorescence signal at slow frame rate without increasing laser power or increase frame rates to capture high speed events...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28164460/development-and-application-of-led-arrays-for-use-in-phototherapy-research
#3
Mohammed A Hadis, Paul R Cooper, Michael R Milward, Patricia C Gorecki, Edward Tarte, James Churm, William M Palin
Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies. 96-well plate format LED arrays (400-850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28164459/photothermal-activation-of-astrocyte-cells-using-localized-surface-plasmon-resonance-of-gold-nanorods
#4
Kyungsik Eom, Seoyoung Hwang, Seunghyeon Yun, Kyung Min Byun, Sang Beom Jun, Sung June Kim
Although it has been revealed that astrocytes, generally known as star-shaped glial cells, play critical roles in the functions of central nervous system, there have been few efforts to directly modulate their activities and responses. In this study, an optical stimulation strategy for producing intracellular Ca(2+) transients of astrocytes is demonstrated using near-infrared (NIR) light and localized surface plasmon resonance. It is presented that NIR stimulation of micro-second duration combined with gold nanorods (GNRs) efficiently produces stronger Ca(2+) transients of astrocytes, which seems to be associated with a local heat generation by photothermal effects of GNRs...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28164445/holographic-optical-tweezers-based-in-vivo-manipulations-in-zebrafish-embryos
#5
Florian Hörner, Robert Meissner, Sruthi Polali, Jana Pfeiffer, Timo Betz, Cornelia Denz, Erez Raz
Understanding embryonic development requires the characterization of the forces and the mechanical features that shape cells and tissues within the organism. In addition, experimental application of forces on cells and altering cell and organelle shape allows determining the role such forces play in morphogenesis. Here, we present a holographic optical tweezers-based new microscopic platform for in vivo applications in the context of a developing vertebrate embryo that unlike currently used setups allows simultaneous trapping of multiple objects and rapid comparisons of viscoelastic properties in different locations...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28164443/low-level-light-emitting-diode-led-therapy-suppresses-inflammasome-mediated-brain-damage-in-experimental-ischemic-stroke
#6
Hae In Lee, Sae-Won Lee, Nam Gyun Kim, Kyoung-Jun Park, Byung Tae Choi, Yong-Il Shin, Hwa Kyoung Shin
Use of photostimulation including low-level light emitting diode (LED) therapy has broadened greatly in recent years because it is compact, portable, and easy to use. Here, the effects of photostimulation by LED (610 nm) therapy on ischemic brain damage was investigated in mice in which treatment started after a stroke in a clinically relevant setting. The mice underwent LED therapy (20 min) twice a day for 3 days, commencing at 4 hours post-ischemia. LED therapy group generated a significantly smaller infarct size and improvements in neurological function based on neurologic test score...
February 6, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28133932/differential-standard-deviation-of-log-scale-intensity-based-optical-coherence-tomography-angiography
#7
Weisong Shi, Wanrong Gao, Chaoliang Chen, Victor X D Yang
In this paper, a differential standard deviation of log-scale intensity (DSDLI) based optical coherence tomography angiography (OCTA) is presented for calculating microvascular images of human skin. The DSDLI algorithm calculates the variance in difference images of two consecutive log-scale intensity based structural images from the same position along depth direction to contrast blood flow. The en face microvascular images were then generated by calculating the standard deviation of the differential log-scale intensities within the specific depth range, resulting in an improvement in spatial resolution and SNR in microvascular images compared to speckle variance OCT and power intensity differential method...
January 30, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28133923/a-comparative-study-of-ex-vivo-skin-optical-clearing-using-two-photon-microscopy
#8
Anton Sdobnov, Maxim E Darvin, Juergen Lademann, Valery Tuchin
Multiphoton tomography (MPT) is a prospective tool for imaging the skin structure. Aiming to increase the probing depth, a comparative ex vivo study of optical clearing of porcine ear skin was performed by using two optical clearing agents (OCAs), i.e., glycerol and iohexol (Omnipaque(TM) ) at different concentrations, which exhibit different osmotic properties. The results show that a topical application of glycerol or Omnipaque(TM) solutions onto the skin for 60 min significantly improved the depth and contrast of the MPT signals...
January 30, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28128537/inverted-multiscale-optical-resolution-photoacoustic-microscopy
#9
Weizhi Qi, Tian Jin, Jian Rong, Huabei Jiang, Lei Xi
Optical resolution photoacoustic microscopy (ORPAM) is one of the fastest evolving photoacoustic imaging modalities. It not only has a comparable spatial resolution to pure optical microscopies such as epifluorescence microscopy, confocal microscopy, and two-photon microscopy, but also owns a higher penetration depth. In this study, we report an inverted multiscale (IM)-ORPAM that utilizes a two-dimensional galvanometric scanner integrated with two microscopic imaging lenses to achieve rotary-scanning-based multiscale imaging...
January 27, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28128535/triglyceride-dependent-differentiation-of-obesity-in-adipose-tissues-by-ftir-spectroscopy-coupled-with-chemometrics
#10
Fatma Kucuk Baloglu, Onur Baloglu, Sebastian Heise, Gudrun Brockmann, Feride Severcan
The excess deposition of triglycerides in adipose tissue is the main reason of obesity and causes excess release of fatty acids to the circulatory system resulting in obesity and insulin resistance. Body mass index and waist circumference are not precise measure of obesity and obesity related metabolic diseases. Therefore, in the current study, it was aimed to propose triglyceride bands located at 1770-1720 cm(-1) spectral region as a more sensitive obesity related biomarker using the diagnostic potential of Fourier Transform Infrared (FTIR) spectroscopy in subcutaneous (SCAT) and visceral (VAT) adipose tissues...
January 27, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28128530/the-effects-of-low-level-laser-therapy-on-both-hiv-1-infected-and-uninfected-tzm-bl-cells
#11
Masixole Yvonne Lugongolo, Sello Lebohang Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa
Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, the quest for new therapies continues. Low level laser therapy (LLLT) involves the exposure of cells to low levels of red or infrared light. LLLT has been widely used in different medical conditions, but not in HIV-1 infection...
January 27, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28106955/cypate-mediated-thermosensitive-nanoliposome-for-tumor-imaging-and-photothermal-triggered-drug-release
#12
Zhihao Han, Liwei Lv, Yi Ma, Zhaohui Wang, Yuxi Liu, Min Zhang, Siwen Li, Yueqing Gu
It is an emerging focus to explore controlled release drug delivery systems for simultaneous cancer imaging and therapy. Herein, we synthesized a photothermal sensitive multifunctional nano-liposome drug delivery system, with doxorubicin wrapped in the hydropholic layer as the therapeutical agent and cypate doped in the hydrophobic layer as the diagnostic agent. A series of in vitro and in vivo characterization demonstrated the stability of synthesized liposome, as the DL% was 9 ± 1.5 and the EE% was 82.7 ± 2...
January 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28106950/confocal-laser-endomicroscopy-in-head-and-neck-malignancies-using-fitc-labelled-epcam-and-egf-r-antibodies-in-cell-lines-and-tumor-biopsies
#13
Anna S Englhard, Alexander Palaras, Veronika Volgger, Herbert Stepp, Brigitte Mack, Darko Libl, Olivier Gires, Christian S Betz
Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas (HNSCC), and represent prognostic and therapeutic tumor target molecules...
January 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28106949/atherectomy-using-a-solid-state-laser-at-355-nm-wavelength
#14
Amir Herzog, Grzegorz Oszkinis, David Planer, Krzysztof Ziaja, Łukasz Kruszyna, Michał Goran Stanisić, Damian Ziaja, Amiel A Ishaaya, Wacław Kuczmik
Peripheral arterial disease (PAD), caused by atherosclerotic processes, is allied with an increased risk of ischemic events, limb loss, and death. Recently, the use of a solid-state laser at 355 nm within a hybrid catheter was suggested for that purpose. In this work, short nanosecond pulses of a solid-state laser at 355 nm delivered through a hybrid catheter, composed of optical fibers and a blunt mechanical blade, are used to conduct a pre-clinical study and two clinical cases. The pre-clinical study consisted of an atherosclerotic calcified cadaveric leg and a porcine in vivo trial within the iliac artery, respectively...
January 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28079304/localized-measurements-of-physical-parameters-within-human-sperm-cells-obtained-with-wide-field-interferometry
#15
Michal Balberg, Mattan Levi, Ksawery Kalinowski, Itay Barnea, Simcha K Mirsky, Natan T Shaked
We developed a new method to identify the separate cellular compartments in the optical path delay (OPD) maps of un-labeled spermatozoa. This was conducted by comparing OPD maps of fixed, un-labeled spermatozoa to bright field images of the same cells following labeling. The labeling enabled us to identify the acrosomal and nuclear compartments in the corresponding OPD maps of the cells. We then extracted the refractive index maps of fixed cells by dividing the OPD maps of spermatozoa by the corresponding thickness maps of the same cells, obtained with AFM...
January 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28067998/multimodal-fiber-probe-spectroscopy-allows-detecting-epileptogenic-focal-cortical-dysplasia-in-children
#16
Suresh Anand, Riccardo Cicchi, Flavio Giordano, Valerio Conti, Anna Maria Buccoliero, Renzo Guerrini, Francesco Saverio Pavone
We evaluated the diagnostic capability of a multimodal spectroscopic approach for classifying normal brain tissue and epileptogenic focal cortical dysplasia in children. We employed fluorescence spectroscopy at two excitation wavelengths (378 nm and 445 nm) and Raman spectroscopy (at 785 nm excitation) for acquiring fluorescence and Raman spectra from 10 normal brains, 16 focal cortical dysplasia specimens and 1 cortical tuber tissue sites using a custom-built multimodal optical point spectroscopic system. We used principal component analysis combined with leave-one-sample-out-cross-validation for tissue classification...
January 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28067994/identification-of-morphological-and-biochemical-changes-in-keratin-8-18-knock-down-cells-using-raman-spectroscopy
#17
S P Singh, Hunain Alam, Crismita Dmello, Hitesh Mamgain, Milind M Vaidya, Ramachandra Rao Dasari, C Murali Krishna
Accurate understanding of cellular processes and responses to stimuli is of paramount importance in biomedical research and diagnosis. Raman spectroscopy (RS), a label-free and nondestructive spectroscopic method has the potential to serve as a novel 'theranostics' tool. Both fiber-optic and micro-Raman studies have demonstrated efficacy in diagnostics and therapeutic response monitoring. In the present study, we have evaluated the potential of micro-Raman spectroscopic maps in identifying changes induced by loss of K8/18 proteins in a tongue cancer cell line...
January 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28067461/phagocytosis-and-immune-response-studies-of-macrophage-nanodiamond-interactions-in-vitro-and-in-vivo
#18
K-J Huang, C-Y Lee, Y-C Lin, C-Y Lin, E Perevedentseva, S-F Hung, C-L Cheng
The applications of nanodiamond as drug delivery and bio-imaging can require the relinquishing ND-drug conjugate via blood flow, where interaction with immune cells may occur. In this work, we investigated the ND penetration in macrophage and the immune response using the tissue-resident murine macrophages (RAW 264.7). Confocal fluorescence imaging, immunofluorescence analysis of nuclear translocation of interferon regulatory factor IRF-3 and transcriptional factor NF-κΒ, analysis of pro-inflammatory cytokines production IL-1β, IL-6 IL-10 with a reverse transcription-polymerase chain reaction technique were applied...
January 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28009135/diagnostics-of-femoral-head-status-in-humans-using-laser-spectroscopy-in-vitro-studies
#19
Huiying Lin, Wansha Li, Hao Zhang, Peng Chen, Delong Chen, Wei He, Sune Svanberg, Katarina Svanberg
Osteonecrosis of the femoral head (ONFH), a recalcitrant and disabling disease, is caused by inadequate or fully disrupted blood supply to the affected segment of the subchondral bone. Theoretically, there will develop gas-filled pores during the bone decay process due to lacking blood supply. Unfortunately, the relationship between the gas-filled pores and ONFH is still unclear. Here, we have introduced diode laser absorption spectroscopy to detect oxygen and water vapor signals in the femoral heads from hip replacement in 19 patients...
December 23, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/28009134/raman-spectroscopy-and-pca-svm-as-a-non-invasive-diagnostic-tool-to-identify-and-classify-qualitatively-glycated-hemoglobin-levels-in-vivo
#20
J F Villa-Manríquez, J Castro-Ramos, F Gutiérrez-Delgado, M A Lopéz-Pacheco, A E Villanueva-Luna
In this study we identify and classify high and low levels of glycated hemoglobin (HbA1c) in healthy volunteers (HV) and diabetic patients (DP). Overall, 86 subjects were evaluated. The Raman spectrum was measured in three anatomical regions of the body: index fingertip, right ear lobe, and forehead. The measurements were performed to compare the difference between the HV and DP (22 well controlled diabetic patients (WCDP) (HbA1c <6.5%), and 49 not controlled diabetic patients (NCDP) (HbA1c ≥6.5%)). Multivariable methods such as principal components analysis (PCA) combined with support vector machine (SVM) were used to develop effective diagnostic algorithms for classification among these groups...
December 23, 2016: Journal of Biophotonics
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