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Journal of Biophotonics

Maddipatla Reddikumar, Joel Cervantes, Yukitoshi Otani, Barry Cense
As data acquisition for retinal imaging with optical coherence tomography (OCT) becomes faster, efficient collection of photons becomes more important to maintain image quality. One approach is to use a larger aperture at the eye's pupil to collect more photons that have been reflected from the retina. A 2.8-mm beam diameter system with only 7 reflecting surfaces was developed for low-loss retinal imaging. The larger beam size requires defocus and astigmatism correction, which was done in a closed loop adaptive optics method using a Shack-Hartmann wavefront sensor and a deformable mirror with 140 actuators and a +/-2...
October 16, 2018: Journal of Biophotonics
Yujin Ohsugi, Akira Aoki, Koji Mizutani, Sayaka Katagiri, Motohiro Komaki, Masahiro Noda, Toru Takagi, Sho Kakizaki, Walter Meinzer, Yuichi Izumi
The Er:YAG laser is currently used for bone ablation. However, the effect of Er:YAG laser irradiation on bone healing remains unclear. The aim of this study was to investigate bone healing following ablation by laser irradiation as compared with bur drilling. Rat calvarial bone was ablated using Er:YAG laser or bur with water coolant. Er:YAG laser effectively ablated bone without major thermal changes. In-vivo micro-CT analysis revealed that laser irradiation showed significantly higher bone repair ratios than bur drilling...
October 15, 2018: Journal of Biophotonics
Jianyi Xu, Yilin Ma, Tingting Yu, Dan Zhu
Various tissue optical clearing techniques have sprung up for large volume imaging. However, there are few methods showed clearing and imaging data on different organs while most of them were focused on mouse brain, and as a result, it is difficult to select the suitable method for organs in practical applications due to lack of quantitative evaluation and comprehensive comparison. Therefore, it is necessary to evaluate and compare the performances of clearing methods for different organs. In this paper, several typical optical clearing methods were applied, including 3DISCO, uDISCO, SeeDB, FRUIT, CUBIC, ScaleS and PACT to clear intact brain, heart, kidney, liver, spleen, stomach, lung, small intestine, skin and muscle...
October 15, 2018: Journal of Biophotonics
Neeru Singla, Kavita Dubey, Vishal Srivastava
The benchmark method for the evaluation of breast cancers involves microscopic testing of a hematoxylin and eosin (H&E) stained tissue biopsy. Resurgery is required in 20-30% of cases because of incomplete excision of malignant tissues. Therefore, a more accurate method is required to detect the cancer margin to avoid the risk of recurrence. In the recent years, convolutional neural networks (CNNs) has achieved excellent performance in the field of medical images diagnosis. It automatically extracts the features from the images and classifies them...
October 14, 2018: Journal of Biophotonics
Mingyi Wang, Nanshou Wu, Hongheng Huang, Jiaxiong Luo, Gongpu Lan, Yaguang Zeng, Xuehua Wang, Honglian Xiong, Dingan Han, Haishu Tan
A large-depth-of-field full-field optical angiography (LD-FFOA) method is developed to expand the depth of field using a contrast pyramid fusion algorithm (CPFA). The absorption intensity fluctuation modulation effect is utilized to obtain full-field optical angiography (FFOA) images at different focus positions. The CPFA is used to process these FFOA images with different focuses. By selecting high-contrast areas, the CPFA can highlight the characteristics and details of blood vessels to obtain LD-FFOA images...
October 13, 2018: Journal of Biophotonics
Etienne Xavier Keller, Vincent De Coninck, Marie Audouin, Steeve Doizi, Dominique Bazin, Michel Daudon, Olivier Traxer
Urinary stones can be readily disintegrated by Holmium:YAG laser (Holmium laser lithotripsy), resulting in a mixture of small stone dust particles which will spontaneously evacuate with urine and larger residual fragments requiring mechanical retrieval. Differences between fragments and dust have not been well characterized. Also, it remains unknown how the recently introduced "Moses technology" may alter stone disintegration products. Three complementary analytical techniques have been used in this study to offer an in-depth characterization of disintegration products after in vitro Holmium laser lithotripsy: stereoscopic microscopy, scanning electron microscopy and Fourier transform infrared spectroscopy...
October 13, 2018: Journal of Biophotonics
Bong Han Lee, Sindhuja Suresh, Andrew Ekpenyong
Quantum dots (QDs) are semiconductor nanoparticles ranging in size from 2-10 nm. QDs are increasingly being developed for biomedical imaging, targeted drug delivery and green energy technology. These have led to much research on QD interactions with various physical, chemical and biological systems. For biological systems, research has focused on the biocompatibility/cytotoxicity of QDs in the context of imaging/therapy. However, there is a paucity of work on how biological systems and bioactive molecules might be used to alter the optoelectronic properties of QDs...
October 13, 2018: Journal of Biophotonics
Chenfei Hu, Richard Sam, Mingguang Shan, Viorel Nastasa, Minqi Wang, Taewoo Kim, Martha Gillette, Parijat Sengupta, Gabriel Popescu
Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior in live organisms. However, detecting the response to the optical stimulation requires electrophysiology with physical contact or fluorescent imaging at target locations, which is often limited by photobleaching and phototoxicity. In this paper, we show that phase imaging can report the intracellular transport induced by optogenetic stimulation. We developed a multimodal instrument that can both stimulate cells with subcellular spatial resolution and detect optical pathlength changes with nanometer scale sensitivity...
October 12, 2018: Journal of Biophotonics
Joakim Glasin, Joakim Henricson, Lars-Göran Lindberg, Daniel Wilhelms
Vital sign assessment is a common task in emergency medicine, but resources for continuous monitoring are restricted, data is often recorded manually, and entangled wires cause frustration. Therefore, we designed a small, wireless photoplethysmographic device capable of continuously assessing pulse, respiratory frequency and oxygen saturation on the sternum and tested the performance and feasibility in an emergency department setting. Fifty (56.3 ± 20.2 years), consenting emergency patients (29 male) were recruited...
October 10, 2018: Journal of Biophotonics
Jyh-Hong Lee, Yuan-Ta Shih, Ming-Liang Wei, Chi-Kuang Sun, Bor-Luen Chiang
Atopic dermatitis (AD) is a cutaneous disease resulting from a defective barrier and dysregulated immune response. The severity scoring of atopic dermatitis (SCORAD) is used to classify AD. Noninvasive imaging approaches supplementary to SCORAD were investigated. Cr:forsterite laser-based microscopy was employed to analyze endogenous third-harmonic generation (THG) and second-harmonic generation (SHG) signals from skin. Imaging parameters were compared between different AD severities. 3D reconstruction of imaged skin layers were performed...
October 9, 2018: Journal of Biophotonics
Feng-Chieh Li, Sheng-Lin Lee, Hung-Ming Lin, Chih-Ju Lin, Sheng-Shun Wang, Yu-Yuan Lee, Su-Yen Lo, Tzu-Lin Sun, Wei-Liang Chen, Wen Lo, Nicholas Horton, Chris Xu, Shu-Jen Chiang, Yang-Fang Chen, Hsuan-Shu Lee, Chen-Yuan Dong
Acetaminophen (APAP) overdose is one of the world's leading causes of drug-induced hepatotoxicity. Although traditional methods such as histological imaging and biochemical assays have been successfully applied to evaluate the extent of APAP-induced liver damage, detailed effect of how APAP overdose affect the recovery of hepatobiliary metabolism remains not completely understood. In this work, we used intravital multiphoton microscopy to image and quantify hepatobiliary metabolism of the probe 6-carboxyfluorescein diacetate (6-CFDA) in APAP-overdose mice...
October 9, 2018: Journal of Biophotonics
Kaiyan Li, Wenxuan Liang, Jessica Mavadia-Shukla, Hyeon-Cheol Park, Dawei Li, Wu Yuan, Suiren Wan, Xingde Li
Endoscopic optical coherence tomography (OCT) is a noninvasive technology allowing for imaging of tissue microanatomies of luminal organs in real time. Conventional endoscopic OCT operates at 1,300 nm wavelength region with a sub-optimal axial resolution limited to 8-20 μm. In this paper we present the first ultrahigh-resolution tethered OCT capsule operating at 800 nm and offering about 3-4 fold improvement of axial resolution (plus enhanced imaging contrast). The capsule uses diffractive optics to manage chromatic aberration over a full ~200 nm spectral bandwidth centering around 830 nm, enabling to achieve super-achromaticity and an axial resolution of ~2...
October 9, 2018: Journal of Biophotonics
Roya Paridar, Moein Mozaffarzadeh, Vijitha Periyasamy, Maryam Basij, Mohammad Mehrmodammadi, Manojit Pramanik, Mahdi Orooji
Delay-and-sum (DAS) is one of the most common algorithms used to construct the photoacoustic images due to its low complexity. However, it results in images with high sidelobes and low resolution. Delay-and-standard-deviation (DASD) weighting factor can improve the contrast of the images compared to DAS. However, it still suffers from high sidelobes. In this work, a new weighting factor, named delay-multiply-and-standard-deviation (DMASD) is introduced to enhance the contrast of the reconstructed images compared to other mentioned methods...
October 9, 2018: Journal of Biophotonics
Krzysztof Czamara, Elzbieta Karnas, Zuzanna Majka, Tomasz Wojcik, Ewa K Zuba-Surma, Malgorzata Baranska, Agnieszka Kaczor
Confocal Raman imaging combined with fluorescence-activated cell sorting was used for in vitro studies of cell cultures to look at biochemical differences between the cells in different cell phases. To answer the question what is the impact of the cell cycle phase on discrimination of pathological cells, the combination of several factors was checked: a confluency of cell culture, the cell cycle dynamics and development of pathology. Confluency of 70% and 100% results in significant phenotypic cell changes that can be also diverse for different batches...
October 8, 2018: Journal of Biophotonics
Ahmad Golaraei, Kamdin Mirsanaye, Yeji Ro, Serguei Krouglov, Margarete K Akens, Brian C Wilson, Virginijus Barzda
Polarization-dependent second-harmonic generation (P-SHG) microscopy is used to characterize molecular nonlinear optical properties of collagen and determine a three dimensional (3D) orientation map of collagen fibers within a pig tendon. C6 symmetry is used to determine the nonlinear susceptibility tensor components ratios in the molecular frame of reference <mml:math xmlns:mml=""> <mml:msubsup> <mml:mi>χ</mml:mi> <mml:mi>zzz</mml:mi> <mml:mfenced> <mml:mn>2</mml:mn> </mml:mfenced> </mml:msubsup> <mml:mo>/</mml:mo> <mml:msubsup> <mml:mi>χ</mml:mi> <mml:mi>zxx</mml:mi> <mml:mfenced> <mml:mn>2</mml:mn> </mml:mfenced> </mml:msubsup> </mml:math> and <mml:math xmlns:mml="http://www...
October 5, 2018: Journal of Biophotonics
Charlotte Verstraete, Sébastien R Mouchet, Thierry Verbiest, Branko Kolaric
In this perspective article, we review the optical study of different biophotonic geometries and biological structures using classical light in linear and nonlinear regime, especially highlighting the link between these morphologies and modern biomedical research. Additionally, the importance of nonlinear optical study in biological research, beyond traditional cell imaging is also highlighted and described. Finally, we present a short introduction regarding nonclassical light and describe the new future perspective of quantum optical study in biology, revealing the link between quantum realm and biological research...
October 4, 2018: Journal of Biophotonics
Henrik Persson, Wojciech Potrzebowski, Katarzyna Potrzebowska, Lena M Svensson
Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of different affinity forms of the integrin LFA-1. It has been suggested that LFA-1 may form clusters, in order to increase the avidity to ICAM-1. However, this hypothesis still seems to be controversial...
September 29, 2018: Journal of Biophotonics
Luana Osório Fernandes, Cláudia Cristina Brainer de Oliveira Mota, Hugo Otávio Oliveira, José Kayque Neves, Leógenes Maia Santiago, Anderson Stevens Leônidas Gomes
Optical coherence tomography (OCT) is one of the most important imaging modalities for biophotonics applications. In this work, an important step towards the clinical use of OCT in dental practice is reported, by following-up patients treated from periodontal disease (PD). 147 vestibular dental sites from 14 patients diagnosed with PD were evaluated prior and after treatment, using a swept-source OCT and two periodontal probes (Florida probe and North Carolina) for comparison. The evaluation was performed at four stages: day 0, day 30, day 60 and day 90...
September 27, 2018: Journal of Biophotonics
Christine M O'Brien, Katherine J Cochran, Laura E Masson, Mack Goldberg, Eric Marple, Kelly A Bennett, Jeff Reese, James C Slaughter, J Michael Newton, Anita Mahadevan-Jansen
Preterm birth (PTB) is the leading cause of neonatal death, however accurate predictions methods do not exist. Detection of early changes in the cervix, an organ that biochemically remodels to deliver the fetus, has potential to predict PTB risk. Researchers have employed light-based methods to monitor biochemical changes in the cervix during pregnancy, however, these approaches required patients to undergo a speculum exam which many patients find uncomfortable and is not standard practice during prenatal care...
September 26, 2018: Journal of Biophotonics
Yao Zheng, Jiajia Chen, Xin Shi, Xinpei Zhu, Jiahao Wang, Limeng Huang, Ke Si, Colin J R Sheppard, Wei Gong
Two photon microscopy (2PM) is one of the most widely used tools for in vivo deep tissue imaging. However, the spatial resolution and penetration depth are still limited due to the strong scattering background. Here we demonstrate a two-photon focal modulation microscopy. By utilizing the modulation and demodulation techniques, background rejection capability is enhanced, thus spatial resolution and imaging penetration depth are improved. Compared with 2PM, the transverse resolution is increased by 70%, while the axial resolution is increased to 2 folds...
September 25, 2018: Journal of Biophotonics
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