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Journal of Biophotonics

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https://www.readbyqxmd.com/read/27896951/hydrogen-peroxide-modulates-energy-metabolism-and-oxidative-stress-in-cultures-of-permanent-human-m%C3%A3-ller-cells-mio-m1
#1
Sven Peters, Max Griebsch, Matthias Klemm, Jens Haueisen, Martin Hammer
In this study the influence of hydrogen peroxide (H2 O2 ) on the redox state, NADH protein binding, and mitochondrial membrane potential in Müller cells is investigated. Cultures of permanent human Müller cells MIO-M1 were exposed to H2 O2 in 75 µM and 150 µM concentration for two hours. Fluorescence emission spectra and lifetimes were measured by two-photon microscopy (excitation wavelength: 740 nm) at the mitochondria which were identified in the microscopic images by their fluorescence properties (spectra and intensity)...
November 29, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27896946/dynamic-laser-speckle-angiography-achieved-by-eigen-decomposition-filtering
#2
Chenxi Li, Ruikang Wang
A new approach is proposed for statistically analysis of laser speckle signals emerged from a living biological tissue based on eigen-decomposition to separate the dynamic speckle signals due to moving blood cells from the static speckle signals due to static tissue components, upon which to achieve angiography of the interrogated tissue in vivo. The proposed approach is tested by imaging mouse ear pinna in vivo, demonstrating its capability of providing detailed microvascular networks with high contrast, and high temporal and spatial resolutions...
November 29, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27892657/effective-delivery-of-porphyrazine-photosensitizers-to-cancer-cells-by-polymer-brush-nanocontainers
#3
Natalia Y Shilyagina, Nina N Peskova, Svetlana A Lermontova, Anna A Brilkina, Vladimir A Vodeneev, Alexander V Yakimasky, Larisa G Klapshina, Irina V Balalaeva
Efficient drug delivery can be assigned to tasks that attract the most acute attention of researchers in the field of anticancer drug design. We have reported the first case of using amphiphilic polymer brushes as nanocontainers for photosensitizer delivery to cancer cells. Regular graft-copolymers of hydrophobic polyimides with hydrophilic polymethacrylic acid side chains were loaded with photosensitive dye tetra(4-fluorophenyl)tetracyanoporphyrazine (Pz) providing a sufficiently stable homogeneous fraction of fluorescent Pz-loaded nanoparticles with a size of 100-150 nm...
November 28, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27875030/the-differentiation-of-oral-soft-and-hard-tissues-using-laser-induced-breakdown-spectroscopy-a-prospect-for-tissue-specific-laser-surgery
#4
Maximilian Rohde, Fanuel Mehari, Florian Klämpfl, Werner Adler, Friedrich-Wilhelm Neukam, Michael Schmidt, Florian Stelzle
Compared to conventional techniques, Laser surgery procedures provide a number of advantages, but may be associated with an increased risk of iatrogenic damage to important anatomical structures. The type of tissue ablated in the focus spot is unknown. Laser-Induced Breakdown-Spectroscopy (LIBS) has the potential to gain information about the type of material that is being ablated by the laser beam. This may form the basis for tissue selective laser surgery. In the present study, 7 different porcine tissues (cortical and cancellous bone, nerve, mucosa, enamel, dentine and pulp) from 6 animals were analyzed for their qualitative and semiquantitative molecular composition using LIBS...
November 22, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27874264/photobiomodulation-in-human-muscle-tissue-an-advantage-in-sports-performance
#5
Cleber Ferraresi, Ying-Ying Huang, Michael R Hamblin
Photobiomodulation (PBM) describes the use of red or near-infrared (NIR) light to stimulate, heal, and regenerate damaged tissue. Both preconditioning (light delivered to muscles before exercise) and PBM applied after exercise can increase sports performance in athletes. This review covers the effects of PBM on human muscle tissue in clinical trials in volunteers related to sports performance and in athletes. The parameters used were categorized into those with positive effects or no effects on muscle performance and recovery...
November 22, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27868394/sensitivity-and-accuracy-of-hybrid-fluorescence-mediated-tomography-in-deep-tissue-regions
#6
Stefanie Rosenhain, Wa'el Al Rawashdeh, Fabian Kiessling, Felix Gremse
Fluorescence-mediated tomography (FMT) enables noninvasive assessment of the three-dimensional distribution of near-infrared fluorescence in mice. The combination with micro-computed tomography (µCT) provides anatomical data, enabling improved fluorescence reconstruction and image analysis. The aim of our study was to assess sensitivity and accuracy of µCT-FMT under realistic in vivo conditions in deeply-seated regions. Accordingly, we acquired fluorescence reflectance images (FRI) and µCT-FMT scans of mice which were prepared with rectal insertions with different amounts of fluorescent dye...
November 21, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27860359/spectral-diffuse-reflectance-and-autofluorescence-imaging-can-perform-early-prediction-of-blood-vessel-occlusion-in-skin-flaps
#7
Shuo Chen, Caigang Zhu, Christopher Hoe-Kong Chui, Gyanendra Sheoran, Bien-Keem Tan, Quan Liu
Flap transfer has become a common technique in reconstructive surgery. However, a significant number of compromised skin flaps are not successfully salvaged because the current clinical method for flap assessment relies heavily on the clinician's experience. Vascular occlusion is the major reason for flap failure, thus the accurate and objective early prediction of blood vessel occlusion is vitally important. Our parallel point measurement study has demonstrated the great potential of joint diffuse reflectance and autofluorescence spectroscopy in the early detection and differentiation of venous and arterial occlusion in skin flaps...
November 17, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27813365/a-method-to-study-the-hemodynamics-of-chicken-embryo-s-aortic-arches-using-optical-coherence-tomography
#8
Zhen Yu Gordon Ko, Kalpesh Mehta, Muhammad Jamil, Choon Hwai Yap, Nanguang Chen
Congenital cardiovascular defects are the leading cause of birth defect related death. It has been hypothesized that fluid mechanical forces of embryonic blood flow affect cardiovascular development and play a role in congenital malformations. Studies in small animal embryos can improve our understanding of congenital malformations and can lead to better treatment. We present a feasibility study in which high-resolution optical coherence tomography (OCT) and computational fluid dynamics (CFD) are combined to provide quantitative analysis of the embryonic flow mechanics and the associated anatomy in a small animal model...
November 4, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27813279/oxygenation-dynamics-of-sepsis-patients-undergoing-far-infrared-intervention-based-on-near-infrared-spectroscopy
#9
Kuei-Hung Chuang, Ming-Lung Chuang, Sung-Kien Sia, Chia-Wei Sun
Near-infrared spectroscopy (NIRS; continuous wave type) is a noninvasive tool for detecting the relative change of oxyhemoglobin and deoxyhemoglobin. To make this change, intervention methods must be applied. This study determined the hemodynamics of 44 healthy participants and 35 patients with sepsis during exposure to FIR as a novel physical intervention approach. Local microcirculation of their brachioradialis was monitored during exposure and recovery through NIRS. The variations in blood flow and microvascular reaction were determined by conducting paired and unpaired t tests...
November 4, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27807933/monochromatic-subdiffusive-spatial-frequency-domain-imaging-provides-in-situ-sensitivity-to-intratumoral-morphological-heterogeneity-in-a-murine-model
#10
David M McClatchy, P Jack Hoopes, Brian W Pogue, Stephen Chad Kanick
For the first time, spatially resolved quantitative metrics of light scattering recovered with sub-diffusive spatial frequency domain imaging (sd-SFDI) are shown to be sensitive to changes in intratumoral morphology and viability by direct comparison to histopathological analysis. Two freshly excised subcutaneous murine tumor cross-sections were measured with sd-SFDI, and recovered optical scatter parameter maps were co-registered to whole mount histology. Unique clustering of the optical scatter parameters μs' vs...
November 3, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27804236/label-free-imaging-of-cell-substrate-contacts-by-holographic-total-internal-reflection-microscopy
#11
Biaggio Mandracchia, Oriella Gennari, Valentina Marchesano, Melania Paturzo, Pietro Ferraro
The study of cell adhesion contacts is pivotal to understand cell mechanics and interaction at substrates or chemical and physical stimuli. We designed and built a HoloTIR microscope for label-free quantitative phase imaging of total internal reflection. Here we show for the first time that HoloTIR is a good choice for label-free study of focal contacts and of cell/substrate interaction as its sensitivity is enhanced in comparison with standard TIR microscopy. Finally, the simplicity of implementation and relative low cost, due to the requirement of less optical components, make HoloTIR a reasonable alternative, or even an addition, to TIRF microscopy for mapping cell/substratum topography...
November 2, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27804226/in-vivo-terahertz-reflection-imaging-of-human-scars-during-and-after-the-healing-process
#12
Shuting Fan, Benjamin S Y Ung, Edward P J Parrott, Vincent P Wallace, Emma Pickwell-MacPherson
We use terahertz imaging to measure four human skin scars in vivo. Clear contrast between the refractive index of the scar and surrounding tissue was observed for all of the scars, despite some being difficult to see with the naked eye. Additionally, we monitored the healing process of a hypertrophic scar. We found that the contrast in the absorption coefficient became less prominent after a few months post-injury, but that the contrast in the refractive index was still significant even months post-injury. Our results demonstrate the capability of terahertz imaging to quantitatively measure subtle changes in skin properties and this may be useful for improving scar treatment and management...
November 2, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27774782/reference-independent-wide-field%C3%A2-fluorescence-lifetime-measurements-using-frequency-domain-fd-technique-based-on-phase-and-amplitude-crossing-point
#13
Gilad Yahav, Eran Barnoy, Nir Roth, Lior Turgeman, Dror Fixler
Fluorescence lifetime imaging microscopy (FLIM) is an essential tool in many scientific fields such as biology and medicine thanks to the known advantages of the fluorescence lifetime (FLT) over the classical fluorescence intensity (FI). However, the frequency domain (FD) FLIM technique suffers from its strong dependence on the reference and its compliance to the sample. In this paper, we suggest a new way to calculate the FLT by using the crossing point (CRPO) between the modulation and phase FLTs measured over several light emitting diode (LED) DC currents values instead of either method alone...
October 24, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27774747/improved-quantification-of-collagen-anisotropy-with-polarization-resolved-second-harmonic-generation-microscopy
#14
Radu Hristu, Stefan G Stanciu, Denis E Tranca, George A Stanciu
Imaging tissue samples by polarization-resolved second harmonic generation microscopy provides both qualitative and quantitative insights into collagen organization in a label-free manner. Polarization-resolved second harmonic generation microscopy goes beyond simple intensity-based imaging by adding the laser beam polarization component and applying different quantitative metrics such as the anisotropy factor. It thus provides valuable information on collagen arrangement not available with intensity measurements alone...
October 24, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27758051/quantitative-phase-filtered-wavelength-modulated-differential-photoacoustic-radar-tumor-hypoxia-imaging-toward-early-cancer-detection
#15
Edem Dovlo, Bahman Lashkari, Sung Soo Sean Choi, Andreas Mandelis, Wei Shi, Fei-Fei Liu
Overcoming the limitations of conventional linear spectroscopy used in multispectral photoacoustic imaging, wherein a linear relationship is assumed between the absorbed optical energy and the absorption spectra of the chromophore at a specific location, is crucial for obtaining accurate spatially-resolved quantitative functional information by exploiting known chromophore-specific spectral characteristics. This study introduces a non-invasive phase-filtered differential photoacoustic technique, wavelength-modulated differential photoacoustic radar (WM-DPAR) imaging that addresses this issue by eliminating the effect of the unknown wavelength-dependent fluence...
October 19, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27753271/intravital-excitation-increases-detection-sensitivity-for-pulmonary-tuberculosis-by-whole-body-imaging-with-%C3%AE-lactamase-reporter-enzyme-fluorescence
#16
Fatemeh Nooshabadi, Hee-Jeong Yang, Yunfeng Cheng, Madeleine S Durkee, Hexin Xie, Jianghong Rao, Jeffrey D Cirillo, Kristen C Maitland
Tuberculosis is a pulmonary disease with an especially high mortality rate in immuno-compromised populations, specifically children and HIV positive individuals. The causative agent, Mycobacterium tuberculosis (Mtb), is a very slow growing and difficult organism to work with, making both diagnosis and development of effective treatments cumbersome. We utilize a fiber-optic fluorescence microendoscope integrated with a whole-body imaging system for in vivo Mtb detection. The system exploits an endogenous enzyme of Mtb (β-lactamase, or BlaC) using a BlaC-specific NIR fluorogenic substrate...
October 18, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27753229/distinction-between-breast-cancer-cell-subtypes-using-third-harmonic-generation-microscopy
#17
Evangelia Gavgiotaki, George Filippidis, Haris Markomanolaki, George Kenanakis, Sofia Agelaki, Vassilis Georgoulias, Irene Athanassakis
Third Harmonic Generation (THG) microscopy as a non-invasive, label free imaging methodology, allows linkage of lipid profiles with various breast cancer cells. The collected THG signal arise mostly from the lipid droplets and the membrane lipid bilayer. Quantification of THG signal can accurately distinguish HER2-positive cells. Further analysis using Fourier transform infrared (FTIR) spectra reveals cancer-specific profiles, correlating lipid raft-corresponding spectra to THG signal, associating thus THG to chemical information...
October 18, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27753225/photoacoustic-microscopy-of-electronic-acupuncture-ea-effect-in-small-animals
#18
Jinge Yang, Dan Wu, Yong Tang, Huabei Jiang
Acupuncture has been an effective treatment for various pain in China for several thousand years. However, the mechanisms underlying this mysterious ancient healing are still largely unknown. Here we applied photoacoustic microscopy (PAM) to investigate brain hemodynamic changes in response to electronic acupuncture (EA) at ST36 (Zusanli). Due to the high optical absorption of blood at 532 nm, PAM could sensitively probe changes in hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) of cortical regions in high resolution...
October 18, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27730745/autofluorescence-flow-sorting-of-breast-cancer-cell-metabolism
#19
Amy T Shah, Taylor M Cannon, James N Higginbotham, Robert J Coffey, Melissa C Skala
Clinical cancer treatment aims to target all cell subpopulations within a tumor. Autofluorescence microscopy of the metabolic cofactors NAD(P)H and FAD has shown sensitivity to anti-cancer treatment response. Alternatively, flow cytometry is attractive for high throughput analysis and flow sorting. This study measures cellular autofluorescence in three flow cytometry channels and applies cellular autofluorescence to sort a heterogeneous mixture of breast cancer cells into subpopulations enriched for each phenotype...
October 12, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27714989/optical-redox-ratio-and-endogenous-porphyrins-in-the-detection-of-urinary-bladder-cancer-a-patient-biopsy-analysis
#20
Scott Palmer, Karina Litvinova, Andrey Dunaev, Ji Yubo, David McGloin, Ghulam Nabi
Bladder cancer is among the most common cancers in the UK and conventional detection techniques suffer from low sensitivity, low specificity, or both. Recent attempts to address the disparity have led to progress in the field of autofluorescence as a means to diagnose the disease with high efficiency, however there is still a lot not known about autofluorescence profiles in the disease. The multi-functional diagnostic system "LAKK-M" was used to assess autofluorescence profiles of healthy and cancerous bladder tissue to identify novel biomarkers of the disease...
October 7, 2016: Journal of Biophotonics
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