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Journal of Biophotonics

Zhendong Guo, Zhe Li, Yuxiao Deng, Sung-Liang Chen
Photoacoustic microscopy (PAM) is a noninvasive imaging technique and is excellent to study structural and functional changes in the microcirculation. In this work, a lipopolysaccharide (LPS)-induced inflammation model in mice is noninvasively evaluated by PAM. PAM is used to image the microvascular structural changes in mice for 8 hours after the LPS with different concentrations is applied. Quantitative analysis of five vessel parameters is conducted, which shows that the rate of reduction in microvasculature is highly dependent on the applied LPS concentrations...
December 4, 2018: Journal of Biophotonics
Vijitha Periyasamy, Nandan Das, Arunima Sharma, Manojit Pramanik
Photoacoustic imaging is a non-invasive imaging technique having the advantages of high optical contrast and good acoustic resolution at improved imaging depths. Light transport in biological tissues is mainly characterized by strong optical scattering and absorption. Photoacoustic microscopy is capable of achieving high resolution images at greater depth compared to conventional optical microscopy methods. In this work, we have developed a high-resolution, acoustic resolution photoacoustic microscopy (AR-PAM) system in the near infra-red (NIR) window II (NIR-II, e...
December 3, 2018: Journal of Biophotonics
Marina V Novoselova, Daniil N Bratashov, Mustafa Sarimollaoglu, Dmitry A Nedosekin, Walter Harringston, J Alex Watts, Mikyung Han, Boris N Khlebtsov, Ekaterina I Galanzha, Dmitry A Gorin, Vladimir P Zharov
Progress in understanding the cell biology and progression of disease depends on advanced imaging and labeling techniques. Here, we address this demand by exploring novel multilayered nanocomposites (MNCs) with plasmonic nanoparticles and adsorbing dyes in thin nonabsorbing shells as supercontrast multimodal photoacoustic (PA) and fluorescent agents in the near-infrared range. The proof of concept was performed with gold nanorods (GNRs) and indocyanine green (ICG) dispersed in a matrix of biodegradable polymers...
December 3, 2018: Journal of Biophotonics
Yi-Hua Huang, Ming-Lung Chuang, Pay-Zen Wang, Yueh-Chi Chen, Chung-Ming Chen, Chia-Wei Sun
Neuromuscular electrical stimulation (NMES) is used for preventing muscle atrophy and improving muscle strength in patients and healthy people. However, the current intensity of NMES is usually set at a level that causes the stimulated muscles to contract. This typically causes pain. Quantifying the instantaneous changes in muscle microcirculation and metabolism during NMES before muscle contraction occurs is crucial, because it enables the current intensity to be optimally tuned, thereby reducing the NMES-induced muscle pain and fatigue...
November 29, 2018: Journal of Biophotonics
Yongliang Wang, Yuanchang Zhao, Anwesha Sarkar, Xuefeng Wang
Nucleases are important enzymes that cleave nucleic acids and play critical roles in DNA repair, immune defense and potentially in cancer invasion. However, their spatial dynamics at subcellular level is much less studied. Here we developed a surface-tethered nuclease sensor (SNS) which directly converts membrane-bound nuclease (MN) activity to fluorescent signal, therefore mapping MN activity on cell adhesion sites with high resolution and sensitivity. With SNS, we studied MN activity on the ventral membrane of cancer cells, where MN activity initially occurs in punctate regions and advances in a coral-shaped pattern...
November 29, 2018: Journal of Biophotonics
Wei Feng, Chao Zhang, Tingting Yu, Oxana Semyachkina-Glushkovskaya, Dan Zhu
The blood-brain barrier (BBB) plays a key role in the health of the central nervous system. Opening the BBB is very important for drug delivery to brain tissues to enhance the therapeutic effect on brain diseases. It is necessary to in vivo monitor the BBB permeability for assessing drug release with high resolution; however, an effective method is lacking. In this work, we developed a new method that combined spectral imaging with an optical clearing skull window to in vivo dynamically monitor BBB opening caused by 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy (PDT), in which the Evans blue dye (EBd) acted as an indicator of the BBB permeability...
November 28, 2018: Journal of Biophotonics
Mengmeng Liu, Xiujie Liu, Zufang Huang, Xiaoqiong Tang, Xueliang Lin, Yunchao Xu, Guannan Chen, Hang Fai Kwok, Yao Lin, Shangyuan Feng
Laser tweezers Raman spectroscopy (LTRS) as a label-free and non-invasive technology has been proven to be an ideal tool for analysis of single living cells which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully employed to examine the colon cancer cells with single base mutation in KRAS gene segment including DKS-8 (KRAS wild-type) and DLD-1 (KRAS mutant-type), HKE-3 (KRAS wild-type) and HCT-116 (KRAS mutant-type)...
November 28, 2018: Journal of Biophotonics
Lu-Wei Wang, Yue Chen, Wei Yan, Xiao-Yu Weng, Zhi-Gang Yang, Tong Ye, Jun-Le Qu
Super-resolution microscopy (SRM) has had a substantial impact on the biological sciences due to its ability to observe tiny objects less than 200 nm in size. Stimulated emission depletion (STED) microscopy represents a major category of these SRM techniques that can achieve diffraction-unlimited resolution based on a purely optical modulation of fluorescence behaviors. Here, we investigated how the laser beams affect fluorescence lifetime in both confocal and STED imaging modes. The results showed that with increasing illumination time, the fluorescence lifetime in two kinds of fluorescent microspheres had an obvious change in STED imaging mode, compared that in confocal imaging mode...
November 28, 2018: Journal of Biophotonics
M Filippi, F Garello, C Pasquino, F Arena, P Giustetto, F Antico, E Terreno
The transplantation of Mesenchymal Stem Cells (MSCs) holds great promise for the treatment of a plethora of human diseases, but new non-invasive procedures are needed to monitor the cell fate in vivo. Already largely used in medical diagnostics, the fluorescent dye Indocyanine Green (ICG) is an established dye to track limited numbers of cells by optical imaging, but it can be visualized also by Photoacoustic Imaging (PAI), which provides a higher spatial resolution than pure near infrared fluorescence imaging (NIRF)...
November 24, 2018: Journal of Biophotonics
Xueliang Lin, Lingna Wang, Huijing Lin, Duo Lin, Jinyong Lin, Xiujie Liu, Sufang Qiu, Yuanji Xu, Guannan Chen, Shangyuan Feng
Modified nucleoside in urine samples is one of the most common biomarkers for cancer screening. Therefore, we developed a novel detection method for modified nucleoside detection in human urine. In this work, the modified nucleoside from real cancer patient's urine samples was firstly separated and purified using the affinity chromatography (AC) technology relying on its specific adsorption capacity. Then, a surface enhanced Raman spectroscopy (SERS) technology with the capable of single molecular detection was employed for sensitively characterizing the biomolecular features of modified nucleoside...
November 16, 2018: Journal of Biophotonics
Shuting Wang, Jing Liu, Jun Q Lu, Wenjin Wang, Safaa A Al-Qaysi, Yaohui Xu, Wenhuan Jiang, Xin-Hua Hu
Methods for rapid and label-free cell assay are highly desired in life science. Single-shot diffraction imaging presents strong potentials to achieve this goal as evidenced by past experimental results using methods such as polarization diffraction imaging flow cytometry. We present here a platform of methods toward solving these problems and results of optical cell model (OCM) evaluations by calculations and analysis of cross-polarized diffraction image (p-DI) pairs. Four types of realistic OCMs have been developed with two prostate cell structures and adjustable refractive index parameters to investigate the effects of cell morphology and index distribution on calculated p-DI pairs...
November 16, 2018: Journal of Biophotonics
Ke Wang, Yu Du, Hongji Liu, Mengyao Gan, Shen Tong, Wenhui Wen, Ziwei Zhuang, Ping Qiu
Osteocytes are the most abundant cells in bone and always the focus of bone research. They are embedded in the highly scattering mineralized bone matrix. Consequently, visualizing osteocytes deep in bone with subcellular resolution poses a major challenge for in vivo bone research. Here we overcome this challenge by demonstrating 3-photon imaging of osteocytes through the intact mouse skull in vivo. Through broadband transmittance characterization, we establish that the excitation at the 1700-nm window enables the highest optical transmittance through the skull...
November 13, 2018: Journal of Biophotonics
Qian Chen, Heng Guo, Weizhi Qi, Qi Gan, Lei Yang, Bowen Ke, Xingxing Chen, Tian Jin, Lei Xi
Hemorrhagic shock, as an important clinical issue, is regarding as a critical disease with a high mortality rate. Unfortunately, existing clinical technologies are inaccessible to assess the hemorrhagic shock via hemodynamics in microcirculation. Here, we propose an ultracompact photoacoustic microscope to assess hemorrhagic shock using a rat model and demonstrate its clinical feasibility by visualizing buccal microcirculation of healthy volunteers. Both functional and morphological features of the microvascular network including concentration of total hemoglobin (CHbT ), number of blood vessels (VN), small vascular density (SVD) and vascular diameter (VD) were derived to assess the microvascular hemodynamics of different organs...
November 12, 2018: Journal of Biophotonics
Sez-Jade Chen, Nattawut Sinsuebphon, Alena Rudkouskaya, Margarida Barroso, Xavier Intes, Xavier Michalet
We introduce a simple new approach for time-resolved multiplexed analysis of complex systems using near-infrared (NIR) dyes, applicable to in vitro and in vivo studies. We show that fast and precise in vitro quantification of NIR fluorophores' short (sub-nanosecond) lifetime and stoichiometry can be done using phasor analysis, a computationally efficient and user-friendly representation of complex fluorescence intensity decays obtained with pulsed laser excitation and time-gated camera imaging. We apply this approach to the study of binding equilibria by Förster resonant energy transfer using two different model systems: primary/secondary antibody binding in vitro and ligand/receptor binding in cell cultures...
November 12, 2018: Journal of Biophotonics
Hongtao Chen, Ning Ma, Keiichiro Kagawa, Shoji Kawahito, Michelle Digman, Enrico Gratton
Widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) measures the fluorescence lifetime of entire images in a fast and efficient manner. We report a widefield FD-FLIM system based on a complementary Metal-Oxide Semiconductor (CMOS) camera equipped with two-tap true correlated double sampling (CDS) lock-in pixels and lateral electric field charge modulators. Due to the fast intrinsic response and modulation of the camera, our system allows parallel multi-frequency Fluorescence Lifetime Imaging Microscopy (FLIM) in one measurement via Fast Fourier Transform...
November 12, 2018: Journal of Biophotonics
Rishikesh Pandey, Renjie Zhou, Rosalie Bordett, Ciera Hunter, Kristine Glunde, Ishan Barman, Tulio Valdez, Christine Finck
Label-free quantitative imaging is highly desirable for studying live cells by extracting pathophysiological information without perturbing cell functions. Here we demonstrate a novel label-free multimodal optical imaging system with the capability of providing comprehensive morphological and molecular attributes of live cells. Our morpho-molecular microscopy (3M) system draws on the combined strength of quantitative phase microscopy (QPM) and Raman microscopy to probe the morphological features and molecular fingerprinting characteristics of each cell under observation...
November 12, 2018: Journal of Biophotonics
Vladimir Y Zaitsev, Alexander L Matveyev, Lev A Matveev, Grigory V Gelikonov, Olga I Baum, Alexander I Omelchenko, Dmitry V Shabanov, Alexander A Sovetsky, Alexey V Yuzhakov, Anatoly A Fedorov, Vladimir I Siplivy, Andrey V Bolshunov, Emil N Sobol
Moderate heating of such collagenous tissues as cornea and cartilages by IR-laser irradiation is an emerging technology for non-destructive modification of the tissue shape and microstructure for a variety of applications in ophthalmology, otolaryngology, etc. Post-irradiation high-resolution microscopic examination indicates the appearance of microscopic either spheroidal or crack-like narrow pores depending on the tissue type and irradiation regime. Such examinations usually require special tissue preparation (e...
November 11, 2018: Journal of Biophotonics
Wenyan Guo, Xiuli Liu, Qinglei Hu, Fei Huang, Ning Li, Qi Zhang, Yuxin Li, Feng Xiong, Qingming Luo, Shaoqun Zeng
Nucleic acids of intact biological tissues are rich in biological information. Whole-mount in situ hybridization is a powerful technique to mine the wealth of data contained in DNAs or RNAs, especially mRNAs. However, there are no simple, rapid approaches to precisely locate mRNAs in whole-mount tissues such as intact brains. By combining the penetration procedures of iDISCO with the signal amplification approach termed hybridization chain reaction, we herein developed a method for whole-brain in situ hybridization at cellular resolution...
November 11, 2018: Journal of Biophotonics
Yubin Liu, Duyang Gao, Mengze Xu, Zhen Yuan
In this study, CuS nanoparticles with optical absorption covering both near-infrared I (NIR-I) and NIR-II biological windows were prepared and served as the contrast agents for multispectral photoacoustic imaging. The physiological parameters including concentrations of deoxyhemoglobin and oxyhemoglobin as well as the water content in the tumor location were quantified based on the multispectral photoacoustic reconstruction method. More importantly, the concentration of CuS nanoparticles /drugs accumulated in the tumor was also recovered after intravenously injection, which are essential for image-guided cancer theranostics...
November 9, 2018: Journal of Biophotonics
Hugh J Byrne, Franck Bonnier, Zeineb Farhane
Two dimensional correlation analysis is explored to data mine the time evolution of the characteristic Raman microspectroscopic signatures of the subcellular responses of the nucleoli of human lung cancer cells to the uptake of doxorubicin. A simulated dataset of experimental control spectra, perturbed with systematically time-dependent spectral changes, constituted by a short term response which represents the initial binding of the drug in the nucleolus, followed by a longer term response of the organelle metabolism, is used to validate the analysis protocol...
November 9, 2018: Journal of Biophotonics
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