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Journal of Biophotonics

Ángela Ruiz-Tórtola, Francisco Prats-Quílez, Daniel Gónzalez-Lucas, María-José Bañuls, Ángel Maquieira, Guy Wheeler, Tamas Dalmay, Amadeu Griol, Juan Hurtado, Helge Bohlmann, Reiner Götzen, Jaime García-Rupérez
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes -upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors- is reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5' termination. Those MBs incorporate a biotin moiety close to their 3' termination in order to selectively bind a streptavidin molecule to them...
April 17, 2018: Journal of Biophotonics
Yanpeng Li, Hui Ma, Lin Gan, Andong Gong, Haibin Zhang, Deming Liu, Qizhen Sun
Escherichia coli (E.coli) bacteria have been found to be responsible for various health outbreaks caused by contaminated food and water. Accurate and rapid test of E.coli is thus crucial for protecting the public health. A fast-response, label-free bacteriophage-based detection of E.coli using multimode microfiber probe is proposed and demonstrated in this paper. Due to the abrupt taper and subwavelength diameter, different modes are excited and guided in the microfiber as evanescent field that can interact with surrounding E...
April 17, 2018: Journal of Biophotonics
Nivedhitha Govindaswamy, Santosh G Gadde, Lavanya Chidambara, Devanshi Bhanushali, Neha Anegondi, Abhijit Sinha Roy
Projection artifacts (PA) affect the quantification of vascular parameters in the deep layer OCT angiography image. This study eliminated PA and quantified its effect on imaging. 53 eyes (30 subjects) of normal Indian subjects and 113 eyes (92 patients) of type 2 diabetes mellitus with retinopathy (DR) underwent imaging with a scan area of 3 mm × 3 mm. In this study, a normalized cross-correlation between superficial and deep layer was used to remove PA in deep layer. Local fractal analysis was done to compute vascular parameters such as foveal avascular zone area (mm2 ), vessel density (%), spacing between large vessels (%) and spacing between small vessels (%)...
April 16, 2018: Journal of Biophotonics
Zhiping Zeng, Jing Ma, Peng Xi, Canhua Xu
In fluctuation-based optical nanoscopy, investigating high-density labeled subcellular structures with high fidelity has been a significant challenge. In this study, based on super-resolution radial fluctuation (SRRF) microscopy, the joint tagging strategy is employed to enable fast high-density nanoscopic imaging and tracking. In fixed cell experiment, multiple types of quantum dots with distinguishable fluorescence spectra are jointly tagged to subcellular microtubules. In each spectral channel, the decrease in labeling density guarantees the high-fidelity super-resolution reconstruction using SRRF microscopy...
April 14, 2018: Journal of Biophotonics
Haichong K Zhang, Ying Chen, Jeeun Kang, Ala Lisok, Il Minn, Martin G Pomper, Emad M Boctor
A sensitive, non-invasive method to detect localized prostate cancer, particularly for early detection and repetitive study in patients undergoing active surveillance, remains an unmet need. Here we propose a molecular photoacoustic (PA) imaging approach by targeting the prostate-specific membrane antigen (PSMA), which is over-expressed in the vast majority of prostate cancers. We performed spectroscopic PA imaging in an experimental model of prostate cancer, namely, in immunocompromised mice bearing PSMA+ (PC3 PIP) and PSMA- (PC3 flu) tumors through administration of the known PSMA-targeted fluorescence agent, YC-27...
April 13, 2018: Journal of Biophotonics
Yan Li, Riqiang Lin, Chengbo Liu, Jianhua Chen, Huadong Liu, Rongqin Zheng, Xiaojing Gong, Liang Song
Endoscopy is an essential clinical tool for the diagnosis of gastrointestinal tract cancer. A photoacoustic system elegantly combining optical and ultrasound endoscopy advantages by providing high-sensitivity functional information and large imaging depth is a potentially powerful tool for gastrointestinal tract imaging. Recently, several photoacoustic endoscopic imaging systems have been proposed and developed. However, the relatively large size and rigid length of the catheter makes it difficult to translate them into wide clinical applications; while the existing system of a relatively small catheter, capable of in vivo animal imaging, is unable to acquire full (360o ) field-of-view cross-section images...
April 10, 2018: Journal of Biophotonics
Xiang Yang, Jia Shi, Yuye Wang, Ke Yang, Xiang Zhao, Guiyu Wang, Degang Xu, Yunxia Wang, Jianquan Yao, Weiling Fu
Timely and accurate bacterial detection is critical for various health and safety applications, which promotes the continuous development of versatile optical sensors for bacterial investigations. Here, we report a new strategy for bacterial colony sensing using terahertz (THz) imaging with minimal assay procedures. The proposed method utilizes the acute sensitivity of THz wave to the changes in the water content and cellular structures. Single bacterial colonies of four bacterial species were directly distinguished using THz imaging by utilizing their differences in THz absorption...
April 6, 2018: Journal of Biophotonics
Bin Yang, Ning-Jiun Jan, Bryn Brazile, Andrew Voorhees, Kira L Lathrop, Ian A Sigal
Collagen fibers play a central role in normal eye mechanics and pathology. In ocular tissues, collagen fibers exhibit a complex three-dimensional (3D) fiber orientation, with both in-plane (IP) and out-of-plane (OP) orientations. Imaging techniques traditionally applied to the study of ocular tissues only quantify IP fiber orientation, providing little information on OP fiber orientation. Accurate description of the complex 3D fiber microstructures of the eye requires quantifying full 3D fiber orientation. Herein, we present 3dPLM, a technique based on polarized light microscopy developed to quantify both IP and OP collagen fiber orientations of ocular tissues...
April 6, 2018: Journal of Biophotonics
Zhenning Chen, Bobby Nadeau, Kevin Yu, Xinxing Shao, Xiaoyuan He, M Cynthia Goh, Anil Kishen
High-resolution deformation measurements in a functionally graded hard tissue such as human dentin is essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully-hydrated dentin in both macro- and microscales remain to be a challenge. This study was conducted in two stages. In stage-1, a stereo-digital image correlation (stereo-DIC) approach was utilized to determine the water-loss and load-induced 3D deformations of teeth in a sagittal section over consecutively acquired frames, from a fully-hydrated state to non-hydrated conditions for a period up to 2 hours...
April 6, 2018: Journal of Biophotonics
Mette Mogensen, Sophie Bojesen, Niels Moeller Israelsen, Michael Maria, Mikkel Jensen, Adrian Podoleanu, Ole Bang, Merete Haedersdal
Optical coherence tomography (OCT) is an optical imaging technology that enables real time, high-resolution, cross-sectional and en face investigation of skin by detecting reflected broad-spectrum near-infrared light from tissue. OCT provides micron-scale spatial resolution and millimeter-scale depth of penetration [1]. Several commercial OCT systems with handheld probes targeted for Dermatology are now available [2].
April 2, 2018: Journal of Biophotonics
Anton A Namykin, Natalia A Shushunova, Maria V Ulanova, Oxana V Semyachkina-Glushkovskaya, Valery V Tuchin, Ivan V Fedosov
The effects of light-driven enhancement of Evans Blue dye complexes with blood plasma proteins were observed for the first time, both in vitro and in vivo. The possible background of the effect concerns the photochemical cis-trans isomerization of the azo dye molecules. The effect was induced in the solution with a red laser with a wavelength of 638 nm, which corresponds to the peak of the dye absorption. The lifetime of the enhanced fluorescence is approximately 1 s and enables its use as an optically tagged molecular flow tracer for blood flow velocity measurements...
March 30, 2018: Journal of Biophotonics
Nadezda A Brazhe, Kirsten Thomsen, Micael Lønstrup, Alexey R Brazhe, Evelina I Nikelshparg, Georgy V Maksimov, Martin Lauritzen, Olga Sosnovtseva
Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm-1 (symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm-1 and 1602 and 1638 cm-1 (vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries...
March 30, 2018: Journal of Biophotonics
Li-Ju Wang, Nicole Naudé, Yu-Chung Chang, Anne Crivaro, Malek Kamoun, Ping Wang, Lei Li
With the rapid development and proliferation of mobile devices with powerful computing power and the ability of integrating sensors into mobile devices, the potential impact of mobile health (mHealth) diagnostics on the public health is drawing researchers' attention. We developed a Smartphone Octo-channel Spectrometer (SOS) as a mHealth diagnostic tool. The SOS has nanoscale wavelength resolution, is self-illuminated from the smartphone itself, and is ultra-low cost (less than $20). A user interface controls the optical sensing parameters and precise alignment...
March 30, 2018: Journal of Biophotonics
Wei Chen, Congwu Du, Yingtian Pan
Swept-source optical coherence tomography has demonstrated the unique advantages for fast imaging rate and long imaging distance, however, limited axial resolution and complex phase noises restrict swept-source optical coherence Doppler tomography (SS-ODT) for quantitative capillary blood flow imaging in the deep cortices. Here, the wavelength-division-multiplexing optical Doppler tomography (WDM-ODT) method, which divides a single interferogram into multiple phase-correlated interferograms, is proposed to effectively enhance the sensitivity for cerebral capillary flow imaging...
March 30, 2018: Journal of Biophotonics
Na Dong, Rolando Berlinguer-Palmini, Ahmed Soltan, Nikhil Ponon, Anthony O'Neil, Andrew Travelyan, Pleun Maaskant, Patrick Degenaar, Xiaohan Sun
Implantable photonic probes are of increasing interest to the field of biophotonics and in particular, optogenetic neural stimulation. Active probes with onboard light emissive elements allow for electronic multiplexing and can be manufactured through existing microelectronics methods. However, as the optogenetics field moves towards clinical practice, an important question arises as to whether such probes will cause excessive thermal heating of the surrounding tissue. Light emitting diodes typically produce more heat than light...
March 30, 2018: Journal of Biophotonics
Iliya V Ivanov, Timo Mappes, Patrick Schaupp, Christian Lappe, Siegfried Wahl
In the eye ultraviolet radiation (UVR) is not known to contribute to visual perception but to mainly damage multiple structures. UVR carries higher energy than visible light and high dose exposure to UVR causes direct cellular damage, which has an important role in the development of cancer. This review provides an overview on the most recent knowledge on the role of UVR in oxidative stress (OS) in relation to non-cancer ocular pathologies: various corneal pathologies, cataract, glaucoma, and age-related macular degeneration...
March 30, 2018: Journal of Biophotonics
Darya N Chernova, Anastasiya I Konokhova, Olga A Novikova, Maxim A Yurkin, Dmitry I Strokotov, Andrei A Karpenko, Andrei V Chernyshev, Valeri P Maltsev
Chylomicrons (CMs) are lipoprotein particles circulating in blood and transporting dietary lipids. Optically speaking, CMs are small compared to the wavelength of visible light and widely distributed by the size and refractive index (RI). Consequently, intensity of light scattered by CMs scales with up to the sixth power of their size, hampering simultaneous analysis of 60 nm and 600 nm CMs. We present an accurate method for quantitative characterization of large-size CM subpopulation by the distributions over size and RI...
March 30, 2018: Journal of Biophotonics
Hongji Liu, Jiaqi Wang, Xiao Peng, Ziwei Zhuang, Ping Qiu, Ke Wang
Multiphoton action cross sections are the prerequisite for excitation light selection. At the 1700-nm window suitable for deep-tissue imaging, wavelength-dependent 3-photon action cross sections ησ3 for RFPs are unknown, preventing wavelength selection. Here we demonstrate: (1) ex vivo measurement of wavelength-dependent ησ3 for purified RFPs; (2) a multiphoton imaging guided measurement system for in vivo measurement; and (3) in vivo measurement of wavelength-dependent ησ3 in RFP labeled cells. These fundamental results will provide guidelines for excitation wavelength selection for 3-photon fluorescence imaging of RFPs at the 1700-nm window, and augment the existing database of multiphoton action cross sections for fluorophores...
March 30, 2018: Journal of Biophotonics
Yuriy Alexandrov, Dino Solar Nikolic, Chris Dunsby, Paul M W French
Förster resonant energy transfer (FRET) measurements are widely used to obtain information about molecular interactions and conformations through the dependence of FRET efficiency on the proximity of donor and acceptor fluorophores. Fluorescence lifetime measurements can provide quantitative analysis of FRET efficiency and interacting population fraction. Many FRET experiments exploit the highly specific labelling of genetically expressed fluorescent proteins, applicable in live cells and organisms. Unfortunately, the typical assumption of fast randomization of fluorophore orientations in the analysis of fluorescence lifetime-based FRET readouts is not valid for fluorescent proteins due to their slow rotational mobility compared to their upper state lifetime...
March 27, 2018: Journal of Biophotonics
Zahra Ghanian, Shima Mehrvar, Nasim Jamali, Nader Sheibani, Mahsa Ranji
Hyperglycemia affects retinal vascular cell function, promotes the development and progression of diabetic retinopathy, and ultimately causes vision loss. Oxidative stress, reactive oxygen species (ROS) in excess, is a key biomarker for diabetic retinopathy. Using time-lapse fluorescence microscopy, ROS dynamics was monitored and the metabolic resistivity of retinal endothelial cells (REC) and pericytes (RPC) was compared under metabolic stress conditions including high glucose (HG). In the presence of a mitochondrial stressor REC exhibited a significant increase in the rate of ROS production compared with RPC...
March 25, 2018: Journal of Biophotonics
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