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Journal of Biophotonics

Honoka Fujii, Sachiko Horie, Kazu Takeda, Shiro Mori, Tetsuya Kodama
The lymphatic drug delivery system (LDDS) is a new technique that permits the injection of drugs into a sentinel lymph node (SLN) at an early stage of tumor metastasis, thereby treating metastasis in the SLN and its secondary lymph nodes (LNs). The quantity of drug required for a LDDS is much smaller than that needed for systemic chemotherapy. However, the relationship between the rate of drug injection into a SLN and the amount of drug reaching the secondary LNs has not been investigated. In this study, we used an MXH10/Mo-lpr/lpr mouse model to show that the optimal rate for the injection of a fluorescent dye by a LDDS was 10-80 μL/min...
February 20, 2018: Journal of Biophotonics
Dorian Hagenah, Alexander Heisterkamp, Stefan Kalies
Femtosecond laser nanosurgery enables precise manipulation of subcellular elements to study regeneration. However, currently it is not frequently employed - probably because of its unknown consequences on the whole cell level. To better understand the associated biological response of the cell, especially in the context of different cell states and cell staining, we manipulated C2C12 myoblasts and myotubes, which were either unstained (NADH signal) or stained with MitoTracker Red. Both signals overlap well and stain similar areas in untreated cells...
February 20, 2018: Journal of Biophotonics
ChunSik Choe, Johannes Schleusener, Jürgen Lademann, Maxim E Darvin
Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth-dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10-30% SC depth), lower NMF concentration in the whole SC, more β-sheet form of keratin (10-90% SC depth), more folded tertiary keratin structures (30-70% SC depth) and higher hexagonal lateral packing order of ICL (10-50% SC depth) compared to human SC in vivo...
February 20, 2018: Journal of Biophotonics
Saiedeh Saghafi, Nikoo Haghi-Danaloo, Klaus Becker, Inna Sabdyusheva, Massih Foroughipour, Christian Hahn, Marko Pende, Martina Wanis, Michael Bergmann, Judith Stift, Balazs Hegedus, Balazs Dome, Hans-Ulrich Dodt
Based on the modal analysis method, we developed a model that describes the output beam of a diode pumped solid state (DPSS) laser emitting a multimode beam. Measuring the output beam profile in the near field and at the constructed far field the individual modes, their respective contributions, and their optical parameters are determined. Using this information, the beam is optically reshaped into a quasi-Gaussian beam by the interference and superposition of the various modes. This process is controlled by a mode modulator unit that includes different meso-aspheric elements and a soft-aperture...
February 19, 2018: Journal of Biophotonics
Yue Li, Lei Zhang, Kehong Long, Hui Gong, Hao Lei
A growing body of literature has suggested that video game playing can induce functional and structural plasticity of the brain. The underlying mechanisms, however, remain poorly understood. In this study, functional near-infrared spectroscopy (fNIRS) was used to record prefrontal activities in 24 experienced game players when they played a massively multiplayer online battle arena (MOBA) video game, League of Legends (LOL), under naturalistic conditions. It was observed that game onset was associated with significant activations in the ventrolateral prefrontal cortex (VLPFC) and concomitant deactivations in the dorsolateral prefrontal cortex (DLPFC) and frontal pole area (FPA)...
February 16, 2018: Journal of Biophotonics
Giuseppe Lombardo, Valentina Villari, Norberto Liborio Micali, Nancy Leone, Cristina Labate, Maria Penelope De Santo, Marco Lombardo
Keratoconus is the primary cause of corneal transplantation in young adults worldwide. Riboflavin/UV-A corneal cross-linking may effectively halt the progression of keratoconus if an adequate amount of riboflavin enriches the corneal stroma and is photo-oxidated by UV-A light for generating additional cross-linking bonds between stromal proteins and strengthening the biomechanics of the weakened cornea. Here we reported a UV-A theranostic prototype device for performing corneal cross-linking with the ability to assess corneal intrastromal concentration of riboflavin and to estimate treatment efficacy in real time...
February 16, 2018: Journal of Biophotonics
Chun Chung, Yu-Pin Chen, Tsai-Hsueh Leu, Chia-Wei Sun
Osteoporosis, defined as decreased bone mineral density (BMD), poses patients in dangers for fracture risk and has become a major public health problem worldwide because of is associated morbidity, mortality and costs. Without doubt, early detection and timely intervention are important to successfully manage osteoporosis and its associated complications. The dual-energy x-ray absorptiometry (DXA) is the most popular and standard method to measure BMD. However, limitations including radiation exposure and availability restrict its application for osteoporosis screening among general population...
February 16, 2018: Journal of Biophotonics
Heng Zhang, Zhenyi Chen, Jiping Wu, Na Chen, Wenjie Xu, Taihao Li, Shupeng Liu
ST36 is a commonly-used acupoint in traditional Chinese medicine (TCM) for treatment of inflammations, pains and gastrointestinal disturbs. For decades,the low power laser acupuncture has been widely applied as an alternative therapy to traditional metal needle acupuncture and achieved relatively fine therapeutic effect for ST36-related symptoms with reduction of uncomfortableness and infection risks. However its disadvantages of low penetrativity and lack of manipulation skills limit its potential performance...
February 15, 2018: Journal of Biophotonics
Tae-Hoon Bok, Eno Hysi, Michael C Kolios
We investigate the optical wavelength dependence in quantitative photoacoustic (QPA) assessment of red blood cell (RBC) aggregation and oxygen saturation (sO2 ) during pulsatile blood flow. Experimentally, the pulsatile flow was imaged with 700-900 nm laser using the Vevo LAZR. Theoretically, the PA signals were computed based on a Green's function integrated with a Monte Carlo simulation of radiant fluence. The pulsatile flow created periodic conditions of RBC aggregation/non-aggregation, altering the aggregate size, and, in turn, the sO2 ...
February 12, 2018: Journal of Biophotonics
Peiru Wang, Jiatong Han, Minglei Wei, Yuting Xu, Guolong Zhang, Haiyan Zhang, Lei Shi, Xiaojing Liu, Michael R Hamblin, Xiuli Wang
5-Aminolevulinic acid photodynamic therapy (ALA-PDT) is known to be effective in the treatment of photoaged skin. However, the molecular mechanisms still remain elusive. Protoporphyrin IX (PpIX) fluorescence is primarily located in the epidermis while ALA-PDT affects the dermal collagen, presumably by an indirect mechanism. This study aimed to investigate the molecular communication in low-dose ALA-PDT occurring between epidermal keratinocytes and dermal fibroblasts. Western blotting and enzyme-linked immunosorbent assays were performed to evaluate collagen expression and transforming growth factor-β (TGF-β) signaling in human keratinocytes and dermal fibroblasts...
February 12, 2018: Journal of Biophotonics
Renzhe Bi, Ghayathri Balasundaram, Seungwan Jeon, Hui Chien Tay, Yang Pu, Xiuting Li, Mohesh Moothanchery, Chulhong Kim, Malini Olivo
The use of an optical resolution photoacoustic microscopy system (OR-PAM) to evaluate the vascular disruptive effect of Combretastatin A4 Phosphate (CA4P) on a murine orthotopic glioma with intact skull is described here. Second generation optical-resolution PAM scanner with a 532 nm pulsed diode-pumped solid-state (DPSS) laser that specifically matches the absorption maximum of haemoglobin in tissues was used to image orthotopic glioma inoculated in mouse brain. 2D maps of brain vasculature with a lateral resolution of 5 μm and a depth of 700 μm at a field of view 5 × 4 mm were acquired on normal brain and glioma brain...
February 8, 2018: Journal of Biophotonics
Miki Haifler, Isaac Pence, Yu Sun, Alexander Kutikov, Robert G Uzzo, Anita Mahadevan-Jansen, Chetan A Patil
Renal mass biopsy is still controversial due to imperfect accuracy(1). Raman spectroscopy (RS) demonstrated promise as an in vivo real-time, non-destructive diagnostic tool in many malignancies. Short wave infra-red (SWIR) RS has the potential to improve on previous RS systems for renal mass diagnosis. The aim of this study is to evaluate a SWIR RS system in differentiating normal and malignant renal samples Measurements were acquired using a benchtop RS system with excitation wavelength at 1064 nm and an InGaAs array detector...
February 7, 2018: Journal of Biophotonics
Pankaj Singh, Gyana Ranjan Sahoo, Asima Pradhan
A spatio-temporal map of human cervical tissue is obtained from time-resolved fluorescence images with the dynamic contrast enhanced through Principal Component Analysis (PCA) for clear demarcation of regions of normal and pre-cancerous conditions. Changes in the properties of fluorescence in different environments are captured through fluorescence lifetime maps in the human cervical tissue sample. The correlation embodied in the 2nd PC representing sectorial information free of background of the 1st PC, segregates fluorescence activities, as illustrated in the PC maps...
February 7, 2018: Journal of Biophotonics
K M Muhammed Shameem, Arun Chawla, Madhukar Mallya, Bijay Kumar Barik, V K Unnikrishnan, V B Kartha, C Santhosh
Presence of renal calculi (kidney stones) in human urethra is being increasingly diagnosed over the last decade and is considered as one of the most painful urological disorders. Accurate analysis of such stones plays a vital role in the evaluation of urolithiasis patients and in turn helps the clinicians toward exact etiologies. Two highly complementary laser-based analytical techniques; Laser Induced Breakdown Spectroscopy (LIBS) and micro-Raman spectroscopy have been used to identify the chemical composition of different types of renal calculi...
February 7, 2018: Journal of Biophotonics
James P Tauber, Christian Matthäus, Claudius Lenz, Dirk Hoffmeister, Jürgen Popp
Basidiomycetes, i.e., mushroom-type fungi, are known to produce pigments in response to environmental impacts. As anti-oxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment production is triggered and pigment secretion is generated at the interaction zone. The identification and analysis of these pigments is important to understand the defense mechanism of fungi, which is essential to counteract an uncontrolled spread of harmful species...
February 7, 2018: Journal of Biophotonics
Biagio Mandracchia, Oriella Gennari, Alessia Bramanti, Simonetta Grilli, Pietro Ferraro
The surface of a c-cut ferroelectric crystal at room temperature is characterized by the so-called screening surface charges, able to compensate the charge due to the spontaneous polarization. Recently, these charges inspired the investigation of the interaction affinity of live cells with lithium niobate and lithium tantalate crystals. However, different knowledge gaps still remain that prevent a reasonable application of these materials for biological applications. Here, a label-free holographic total internal reflection (HoloTIR) microscopy is shown; the technique is able to evaluate quantitatively the contact area of live fibroblast cells adhering onto the surface of a ferroelectric lithium niobate crystal...
February 6, 2018: Journal of Biophotonics
Chih-Ju Lin, Sheng-Lin Lee, Wei-Hsiang Wang, Vladimir A Hovhannisyan, Yao-De Huang, Hsuan-Shu Lee, Chen-Yuan Dong
In this study, intravital multiphoton microscopy was used to study quantitatively investigate hepatobiliary metabolism in chronic pathologies of the liver. Specifically, through the use of the probe molecule 6-carboxyfluorescein diacetate (6-CFDA), the effects of liver fibrosis, fatty liver, and hepatocellular carcinoma on the metabolic capabilities of mouse liver were investigated. After the acquisition of time lapse images, a first order kinetic model was used to calculate rate constant resolved images of various pathologies...
February 1, 2018: Journal of Biophotonics
Martin Kögler, Yury V Ryabchikov, Sanna Uusitalo, Alexey Popov, Anton Popov, Gleb Tselikov, Anna-Liisa Välimaa, Ahmed Al-Kattan, Jussi Hiltunen, Riitta Laitinen, Peter Neubauer, Igor Meglinski, Andrei V Kabashin
The ability of noble metal-based nanoparticles (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as Surface Enhanced Raman Scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional nanoparticles synthesized by colloidal chemistry are typically contaminated by non-biocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification...
February 1, 2018: Journal of Biophotonics
Yubin Liu, Lingyan Zhang, Sushu Li, Xinai Han, Zhen Yuan
Scleroderma (SD) is a rare autoimmune disease, which is divided into two categories: the localized SD and systemic SD. The localized SD mainly causes skin thickening of the fingers, whereas the systemic SD can further affect the blood vessels and internal organs. In this pilot study, the multispectral photoacoustic elastic tomography (PAET) imaging technique was used to recover the quantitative physiological and elastic properties of biological tissues for the diagnosis of SD. Three healthy subjects and three scleroderma patients were recruited and clinically examined by a rheumatologist, and then their hand /fingers were scanned by both MRI and our home-made photoacoustic imaging system...
February 1, 2018: Journal of Biophotonics
Rani Toll John, Joakim Henricson, Johan Junker, Carl-Oscar Jonson, Gert E Nilsson, Daniel Wilhelms, Chris D Anderson
OBJECTIVE: To describe the effect of low ambient temperature on skin temperature and capillary refill (CR) time in forehead, sternum and finger pulp. METHODS: An observational, nonrandomized experimental study on 15 healthy subjects (6 females) in a cold room (8 °C). Outcome measures were skin temperature and quantified CR test after application of a standardized blanching pressure (9 N/cm2 ) using digital photographic polarization spectroscopy to generate CR times...
January 31, 2018: Journal of Biophotonics
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