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Journal of Biophotonics

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https://www.readbyqxmd.com/read/28731623/concentric-photothermal-coagulation-with-basket-integrated-optical-device-for-treatment-of-tracheal-stenosis
#1
Van Nam Tran, Hyungshin Lee, Van Gia Truong, Yun-Hee Rhee, Hyun Wook Kang
A basket-integrated optical device is developed to consistently treat tubular tissue by centering an optical diffuser in the lumen. Four nitinol arms in conjunction with the optical diffusing applicator are deployed to induce homogeneous circumferential light emission and concentric photothermal coagulation on tracheal tissue. 1470-nm laser light is employed for the tissue testing at various irradiation conditions and evaluated in terms of thermal gradient and temperature evolution. Preliminary experiments on liver tissue demonstrate the concentric development of the radial thermal coagulation in the tissue (eccentric ratio = ~5...
July 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28731618/controlling-the-near-infrared-transparency-of-costal-cartilage-by-impregnation-with-clearing-agents-and-magnetite-nanoparticles
#2
Yulia Alexandrovskaya, Kirill Sadovnikov, Andrey Sharov, Anna Sherstneva, Evgeniy Evtushenko, Alexander Omelchenko, Mariya Obrezkova, Valery Tuchin, Valery Lunin, Emil Sobol
Penetration depth of near infrared laser radiation to costal cartilage is controlled by the tissue absorption and scattering and it is the critical parameter to provide the relaxation of mechanical stress throughout the whole thickness of cartilage implant. To enhance the penetration for the laser radiation on 1.56 μm the optical clearing solutions of glycerol and fructose of various concentrations are tested. The effective and reversible tissue clearance was achieved. However, the increasing absorption of radiation should be concerned: 5-8(ᴼ) C increase of tissue temperature was detected...
July 21, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28727323/indocyanine-green-near-infrared-lymphangiography-for-evaluation-of-effectiveness-of-edema-fluid-flow-under-therapeutic-compression
#3
Marzanna T Zaleska, Waldemar L Olszewski
The commonly used modalities for therapy of limb lymphedema are manual lymphatic drainage, manual devices moving edema fluid and intermittent pneumatic compression (IPC). What seems to be necessary for validation of the effect of the compression procedure is imaging of the mobilized moving edema fluid. Picture of edema fluid flow would allow the therapist to use force adjusted to the tissue volume and stiffness differing in various limb regions as well as identify sites of abundant accumulation of fluid requiring more compression...
July 20, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28715124/chemical-imaging-of-fresh-vascular-smooth-muscle-cell-response-by-epi-detected-stimulated-raman-scattering
#4
Delong Zhang, Wei Chen, Huan Chen, Han-Qing Yu, Ghassan Kassab, Ji-Xin Cheng
An understanding of deformation of cardiovascular tissue under hemodynamic load is crucial for understanding the health and disease of blood vessels. In the present work, an epi-detected stimulated Raman scattering (epi-SRS) imaging platform was designed for in situ functional imaging of vascular smooth muscle cells (VMSCs) in fresh coronary arteries. For the first time, the pressure-induced morphological deformation of fresh VSMCs was imaged with no fixation and in a label free manner. The relation between the loading pressure and the morphological parameters, including angle and length of the VSMCs, were apparent...
July 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28715120/characterization-of-a-multiple-particle-delivery-complex-and-determination-of-cellular-photodamage-in-skin-fibroblast-and-breast-cancer-cell-lines
#5
Ivan Mfouo-Tynga, Nicolette Nadene Houreld, Heidi Abrahamse
Zinc metallized Phthalocyanine (ZnPcSmix ), a potent photosensitizer (PS), is conjugated to gold dendrimer encapsulated nanoparticles (AuDENPs) in order to improve the efficacy of Photodynamic therapy (PDT) using MCF-7 breast cancer cells and WS1 fibroblast cells as a control. Both ZnPcSmix and AuDENPs are mixed in a nitrogen atmosphere for 48 h and characterization analysis conducted using ultra violet visible (UV-vis) spectrometry for spectral properties, transmission electron microscopy (TEM) for morphological features and zeta potential measurement for surface stability and size distribution of the compound obtained or of the multiple particles delivery complex (MPDC)...
July 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28708329/resting-state-functional-connectivity-measured-by-diffuse-correlation-spectroscopy
#6
LETTER
Jun Li, Chien-Sing Poon, Jeremy Kress, Daniel J Rohrbach, Ulas Sunar
Near-infrared diffuse correlation spectroscopy is used to record spontaneous cerebral blood flow fluctuations in the frontal cortex. Nine adult subjects participated in the experiments, in which 8-min spontaneous fluctuations were simultaneously recorded from the left and right dorsolateral and inferior frontal regions. Resting-state functional connectivity was measured by the temporal correlation of the low frequency fluctuations. Our data shows the resting-state functional connectivity within the dorsolateral region is significantly stronger than that between the inferior and dorsolateral regions, in line with previous observations with functional near-infrared spectroscopy...
July 14, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700146/in-vivo-thz-imaging-of-human-skin-accounting-for-occlusion-effects
#7
Qiushuo Sun, Edward P J Parrott, Yuezhi He, Emma Pickwell-Macpherson
In vivo terahertz (THz) imaging of human skin needs to be done in reflection geometry due to the high attenuation of THz light by water in the skin. To aid the measurement procedure, there is typically an imaging window onto which the patient places the area of interest. The window enables better pulse alignment and helps keep the patient correctly positioned during the measurement. In this paper we demonstrate how the occlusion caused by the skin contact with the imaging window during the measurement affects the THz response...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700145/simultaneous-measurements-of-lymphatic-vessel-contraction-flow-and-valve-dynamics-in-multiple-lymphangions-using-optical-coherence-tomography
#8
Cedric Blatter, Eelco F J Meijer, Timothy P Padera, Benjamin J Vakoc
Lymphatic dysfunction is involved in many diseases including lymphedema, hypertension, autoimmune responses, graft rejection, atherosclerosis, microbial infections, cancer and cancer metastasis. Expanding our knowledge of lymphatic system function can lead to a better understanding of these disease processes and improve treatment options. Here, optical coherence tomography (OCT) methods were used to reveal intraluminal valve dynamics in 3D, and measure lymph flow and vessel contraction simultaneously in three neighboring lymphangions of the afferent collecting lymphatic vessels to the popliteal lymph node in mice...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700144/in-vivo-full-field-functional-optical-hemocytometer
#9
Fuli Zhang, Mingyi Wang, Dingan Han, Haishu Tan, Guojian Yang, Yaguang Zeng
We present an in vivo lab-free full-field functional optical hemocytometer (FFOH) for application to the capillaries of a live biological specimen, based on the absorption intensity fluctuation modulation (AIFM) effect. Because of the absorption difference between the red blood cells (RBCs) and background tissue under low-coherence light illumination, an endogenous instantaneous intensity fluctuation is generated by the AIFM effect when RBCs discontinuously traverse the capillary. The AIFM effect is used to highlight the RBC signal relative to the background tissue by computing the real-time modulation depth...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700142/developing-raman-spectroscopy-as-a-diagnostic-tool-for-label-free-antigen-detection
#10
Aaran T Lewis, Riana Gaifulina, Naomi J Guppy, Martin Isabelle, Jennifer Dorney, Gavin R Lloyd, Manuel Rodriguez-Justo, Catherine Kendall, Nicholas Stone, Geraint M Thomas
For several decades, a multitude of studies have documented the ability of Raman spectroscopy (RS) to differentiate between tissue types and identify pathological changes to tissues in a range of diseases. Furthermore, spectroscopists have illustrated that the technique is capable of detecting disease-specific alterations to tissue before morphological changes become apparent to the pathologist. This study draws comparisons between the information that is obtainable using RS alongside immunohistochemistry (IHC); since histological examination is the current GOLD standard for diagnosing a wide range of diseases...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700140/development-of-a-molecular-bio-switch-using-fluorescence-lifetime-imaging-incremental-activation-of-fluorescein-diacetate
#11
Eran A Barnoy, Rachela Popovtzer, Dror Fixler
Molecular bio-switches offer an invaluable asset in the shift from systemic to targeted treatments. Within the growing arsenal of switches are imaging probes that functionalize only in given locations or situations. Acetate esters are a common fluorescent example, known to activate upon interaction with esterases. Fluorescein diacetate (FDA) is one such fluorophore used in cell viability assays. These assays rely on the fact that the compound begins colorless and with no fluorescent signature whatsoever, and only after internalization into cells it is possible to detect a fluorescence signal...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700139/oct-layered-tomography-of-the-cornea-provides-new-insights-on-remodeling-after-photorefractive-keratectomy
#12
Rachana S Chandapura, Rohit Shetty, Rushad Shroff, Neha Shilpy, Mathew Francis, Abhijit Sinha Roy
OCT topography of corneal layers was generated to analyze the remodeling of the epithelium and stroma after photorefractive keratectomy (PRK). Myopic PRK was performed in 15 patients. One eye underwent manual scraping of epithelium while the other was treated with Epi clear™. Epi clear allowed a gentler removal of the epithelium compared to manual scraping. Scheimpflug (Pentacam™, OCULUS Optikgerate Gmbh, Germany) and OCT (RTVue™, Optovue Inc., USA) scan of the cornea was performed before and after PRK (3 months)...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700137/dual-led-based-multichannel-microscopy-for-whole-slide-multiplane-multispectral-and-phase-imaging
#13
Jun Liao, Zhe Wang, Zibang Zhang, Zichao Bian, Kaikai Guo, Aparna Nambiar, Yutong Jiang, Shaowei Jiang, Jingang Zhong, Michael Choma, Guoan Zheng
We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for parallel data acquisition, achieving a maximum data throughput of ~1 gigapixel per second. To perform single-frame rapid autofocusing, we place two near-infrared LEDs at the back focal plane of the condenser lens to illuminate the sample from two different incident angles. A hot mirror is used to direct the near-infrared light to an autofocusing camera...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700136/an-automated-framework-to-quantify-areas-of-regional-ischemia-in-retinal-vascular-diseases-with-oct-angiography
#14
Neha Anegondi, Lavanya Chidambara, Devanshi Bhanushali, Santosh G K Gadde, Naresh Kumar Yadav, Abhijit Sinha Roy
In this observational and cross-sectional study, capillary non perfusion (CNP) and vascular changes in branch retinal vein occlusion (BRVO, sample size (n) = 26) and choroidal neovascularization (CNV, n = 29) were evaluated. Subjects underwent imaging using Optical coherence tomography angiography (Angiovue™ OCTA , RTVue XR, Optovue Inc., Fremont, CA). Local fractal analysis was applied to the OCTA images of superficial, deep and choriocapillaries layer. CNP area (BRVO eyes) and vascular parameters were computed using local fractal based method...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700135/a-hybrid-clustering-algorithm-for-multiple-source-resolving-in-bioluminescence-tomography
#15
Hongbo Guo, Jingjing Yu, Zhenhua Hu, Huangjian Yi, Yuqing Hou, Xiaowei He
Bioluminescence tomography is a preclinical imaging modality to locate and quantify internal bioluminescent sources from surface measurements, which experienced rapid growth in the last ten years. However, multiple-source resolving remains a challenging issue in BLT. In this paper, it is treated as an unsupervised pattern recognition problem based on the reconstruction result, and a novel hybrid clustering algorithm combining the advantages of affinity propagation (AP) and K-means is developed to identify multiple sources automatically...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700134/dual-fluorescence-nano-conjugates-for-ratiometric-detection-of-reactive-oxygen-species-in-inflammatory-cells
#16
Yuxiang Ma, Qian Wang, Qiuyun Zhu, Mengyang Liu, Dan Chen, Zhihao Han, Congying Zhang, Liwei Lv, Yueqing Gu
Reactive oxygen species (ROS) are largely produced under pathological situations. To understand the etiology of disease, it is urgent to develop efficacious probes for detecting ROS. Herein, a novel nano-conjugate detection system constructed from gold clusters (AuNCs) and quantum dots (QDs) for fluorescence ratio metric sensing ROS was reported. Upon interacting with ROS, the red emission fluorescence (645 nm from QDs) in the detection system was gradually decreased, while the green fluorescence (480 nm from AuNCs) changed little...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700133/a-possible-ftir-based-plasma-fingerprint-of-ace-i-induced-reversal-of-endothelial-dysfunction-in-diabetic-mice
#17
Emilia Staniszewska-Slezak, Ewelina Wiercigroch, Andrzej Fedorowicz, Elżbieta Buczek, Lukasz Mateuszuk, Malgorzata Baranska, Stefan Chlopicki, Kamilla Malek
Angiotensin-converting enzyme inhibitors (ACE-I) display vasoprotective activity and represent the cornerstone in the treatment of cardiovascular diseases. In the present work we tested whether FTIR-based analysis of blood plasma is sensitive to detect vasoprotective effects of treatment with perindopril including reversal of endothelial dysfunction in diabetes. For this purpose, a plasma samples were collected from untreated db/db mice, db/db mice treated with 2 or 10 mg/kg perindopril and db + mice...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700132/non-invasive-sentinel-lymph-node-mapping-and-needle-guidance-using-clinical-handheld-photoacoustic-imaging-system-in-small-animal
#18
Kathyayini Sivasubramanian, Vijitha Periyasamy, Manojit Pramanik
Translating photoacoustic imaging into clinical setup is a challenge. Handheld clinical real-time photoacoustic imaging systems are not common. In this work, we report an integrated photoacoustic and clinical ultrasound imaging system by combining light delivery with the ultrasound probe for sentinel lymph node imaging and needle guidance in small animal. The open access clinical ultrasound platform allows seamless integration of photoacoustic imaging resulting in the development of handheld real-time photoacoustic imaging probe...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700131/high-intensity-focused-ultrasound-hifu-and-phase-sensitive-optical-coherence-tomography-phs-oct-for-high-resolution-surface-acoustic-wave-saw-elastography
#19
Kanheng Zhou, Nhan Le, Zhihong Huang, Chunhui Li
Elastography has the ability of quantitatively evaluating the mechanical properties of soft tissue, thus is helpful for diagnosis and treatment monitoring for many diseases, e.g. skin diseases. Surface acoustic waves (SAWs) have been proven to be a non-invasive, non-destructive method for accurate characterization of tissue elastic properties. Current surface acoustic wave elastography using high energy laser pulse or mechanical shaker still have some problems. In order to improve SAW elastography in medical application, a new technique was proposed in this paper, which combines high intensity focused ultrasound (HIFU) as a SAWs impulse inducer and PhS-OCT as a SAWs detector...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28700130/photostimulation-effects-on-chicken-eggs-development-perspectives-to-human-newborns-treatment
#20
Hilde Harb Buzzá, Amanda Cristina Zangirolami, Cristina Kurachi, Vanderlei Salvador Bagnato
It is well known that, under exposure to bright light, eggs tend to hatch earlier than control, without causing any damage to the birds. This report has the aim to systematically show the effect and establishes a proposal for a possible application to accelerate the chicken eggs formation that could be extrapolated or adapted as a great advance in premature human newborns. Comparing several protocols, the experiments show that lower doses slowly delivered for 24 hours promote higher efficiency in embryo development, increasing on average 25% of its size and more than 70% in weight when compared to the control...
July 12, 2017: Journal of Biophotonics
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