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Forensic Science International. Genetics

Huijie Lu, Pingming Qiu, Chao Liu, Weian Du, Ling Chen
No abstract text is available yet for this article.
November 21, 2016: Forensic Science International. Genetics
Irena Zupanič Pajnič, Tomaž Zupanc, Jože Balažic, Živa Miriam Geršak, Oliver Stojković, Ivan Skadrić, Matija Črešnar
Human-specific quantitative PCR (qPCR) has been developed for forensic use in the last 10 years and is the preferred DNA quantification technique since it is very accurate, sensitive, objective, time-effective and automatable. The amount of information that can be gleaned from a single quantification reaction using commercially available quantification kits has increased from the quantity of nuclear DNA to the amount of male DNA, presence of inhibitors and, most recently, to the degree of DNA degradation. In skeletal remains samples from disaster victims, missing persons and war conflict victims, the DNA is usually degraded...
November 19, 2016: Forensic Science International. Genetics
Pankaj Shrivastava, Toshi Jain, Veena Ben Trivedi
No abstract text is available yet for this article.
November 11, 2016: Forensic Science International. Genetics
Sander Willems, Senne Cornelis, Yannick Gansemans, Bart Broeckx, Dieter I M De Coninck, Christophe Van Neste, Maarten Dhaenens, Dieter Deforce, Filip Van Nieuwerburgh
No abstract text is available yet for this article.
November 9, 2016: Forensic Science International. Genetics
Peter Gill
No abstract text is available yet for this article.
November 2, 2016: Forensic Science International. Genetics
Eun Young Lee, Hwan Young Lee, So Yeun Kwon, Yu Na Oh, Woo Ick Yang, Kyoung-Jin Shin
In forensic science and human genetics, Y-chromosomal short tandem repeats (Y-STRs) have been used as very useful markers. Recently, more Y-STR markers have been analyzed to enhance the resolution power in haplotype analysis, and 13 rapidly mutating (RM) Y-STRs have been suggested as revolutionary tools that can widen Y-chromosomal application from paternal lineage differentiation to male individualization. We have constructed two multiplex PCR sets for the amplification of 13 RM Y-STRs, which yield small-sized amplicons (<400bp) and a more balanced PCR efficiency with minimum PCR cycling...
October 27, 2016: Forensic Science International. Genetics
M van den Berge, T Sijen
In forensics, DNA profiling is used for the identification of the donor of a trace, while messenger RNA (mRNA) profiling can be applied to identify the cellular origin such as body fluids or organ tissues. The presence of male cell material can be readily assessed by the incorporation of Y-chromosomal markers in quantitation or STR profiling systems. However, no forensic marker exists to positively identify female cell material; merely the presence of female DNA is deduced from the absence of a Y peak, or unbalanced X-Y signals at the Amelogenin locus or unbalanced response of the total and Y-specific quantifier...
October 26, 2016: Forensic Science International. Genetics
Cinthia Bachir Moysés, Weslley Mottoi Tsutsumida, Paulo Eduardo Raimann, Carlos Henrique Ares Silveira da Motta, Tatiana Lúcia Santos Nogueira, Olívia Cristina Lima Dos Santos, Bruno Boiko Pereira de Figueiredo, Thaty Fumiko Mishima, Ian Marques Cândido, Neide Maria de Oliveira Godinho, Laiana Silveira Beltrami, Rochele Kovalski Lopes, Altamir Frederico Guidolin, Adelar Mantovani, Sandra Maria Dos Santos, Carlos Antonio de Souza, Leonor Gusmão
No abstract text is available yet for this article.
October 25, 2016: Forensic Science International. Genetics
N E C Weiler, K Baca, D Ballard, F Balsa, M Bogus, C Børsting, F Brisighelli, J Červenáková, L Chaitanya, M Coble, V Decroyer, S Desmyter, K J van der Gaag, K Gettings, C Haas, J Heinrich, M João Porto, A J Kal, M Kayser, A Kúdelová, N Morling, A Mosquera-Miguel, F Noel, W Parson, V Pereira, C Phillips, P M Schneider, D Syndercombe Court, M Turanska, A Vidaki, P Woliński, L Zatkalíková, T Sijen
A collaborative European DNA Profiling (EDNAP) Group exercise was undertaken to assess the performance of an earlier described SNaPshot™-based screening assay (denoted mini-mtSNaPshot) (Weiler et al., 2016) [1] that targets 18 single nucleotide polymorphism (SNP) positions in the mitochondrial (mt) DNA control region and allows for discrimination of major European mtDNA haplogroups. Besides the organising laboratory, 14 forensic genetics laboratories were involved in the analysis of 13 samples, which were centrally prepared and thoroughly tested prior to shipment...
October 24, 2016: Forensic Science International. Genetics
André Zoratto Gastaldo, Rodrigo Rodenbusch, Rosina Fossati, Carlos Javier Azambuja, Clarice Sampaio Alho
No abstract text is available yet for this article.
October 24, 2016: Forensic Science International. Genetics
James Chun-I Lee, Bill Tseng, Liang-Kai Chang, Adrian Linacre
The development of massively parallel sequencing (MPS) has increased greatly the scale of DNA sequencing. The analysis of massive data-files from single MPS analysis can be a major challenge if examining the data for potential polymorphic loci. To aid in the analysis of both short tandem repeat (STR) and single nucleotide polymorphisms (SNP), we have designed a new program called SEQ Mapper to search for genetic polymorphisms within a large number of reads generated by MPS. This new program has been designed to perform sequence mapping between reference data and generated reads...
October 20, 2016: Forensic Science International. Genetics
Gustavo Martínez, Alicia Borosky, Daniel Corach, Cintia Llull, Laura Locarno, Mercedes Lojo, Miguel Marino, María Cecilia Miozzo, Nidia Modesti, Carla Pacharoni, Juan Pablo Pilili, María Isabel Ramella, Andrea Sala, Cecilia Schaller, Carlos Vullo, Ulises Toscanini
Currently, autosomal Short Tandem Repeat (STR) markers represent the method of election in forensic human identification. Commercial kits of most common use nowadays -e.g. PowerPlex(®)Fusion, Promega Corp.; AmpFlSTR GlobalFiler, Thermofisher scientific; Investigator 24Plex QS,Qiagen-, allow the co-amplification of 23 highly polymorphic STR loci providing a high discrimination power in human identity testing. However, in complex kinship analysis and familial database searches involving distant relationships, additional DNA typing is often required in order to achieve well-founded conclusions...
October 18, 2016: Forensic Science International. Genetics
Yutong Song, Junhong Liu, Ruxin Zhu, Yuan Lin, Yan Liu, Li Li
No abstract text is available yet for this article.
October 18, 2016: Forensic Science International. Genetics
Andreas O Tillmar, Chris Phillips
Advances in massively parallel sequencing technology have enabled the combination of a much-expanded number of DNA markers (notably STRs and SNPs in one or combined multiplexes), with the aim of increasing the weight of evidence in forensic casework. However, when data from multiple loci on the same chromosome are used, genetic linkage can affect the final likelihood calculation. In order to study the effect of linkage for different sets of markers we developed the biostatistical tool ILIR, (Impact of Linkage on forensic markers for Identity and Relationship tests)...
October 15, 2016: Forensic Science International. Genetics
Anna Juras, Maciej Chyleński, Marta Krenz-Niedbała, Helena Malmström, Edvard Ehler, Łukasz Pospieszny, Sylwia Łukasik, Józef Bednarczyk, Janusz Piontek, Mattias Jakobsson, Miroslawa Dabert
We applied an interdisciplinary approach to investigate kinship patterns and funerary practices during the middle Neolithic. Genetic studies, radiocarbon dating, and taphonomic analyses were used to examine two grave clusters from Krusza Zamkowa, Poland. To reconstruct kinship and determine biological sex, we extracted DNA from bones and teeth, analyzed mitochondrial genomes and nuclear SNPs using the HID-Ion AmpliSeq™ Identity panel generated on Illumina and Ion Torrent platforms, respectively. We further dated the material (AMS (14)C) and to exclude aquatic radiocarbon reservoir effects, measures of carbon and nitrogen stable isotopes for diet reconstruction were used...
October 14, 2016: Forensic Science International. Genetics
K Slooten
Several methods exist to compute the likelihood ratio LR(M, g) evaluating the possible contribution of a person of interest with genotype g to a mixed trace M. In this paper we generalize this LR to a likelihood ratio LR(M1, M2) involving two possibly mixed traces M1 and M2, where the question is whether there is a donor in common to both traces. In case one of the traces is in fact a single genotype, then this likelihood ratio reduces to the usual LR(M, g). We explain how our method conceptually is a logical consequence of the fact that LR calculations of the form LR(M, g) can be equivalently regarded as a probabilistic deconvolution of the mixture...
October 12, 2016: Forensic Science International. Genetics
Diana W Williams, Greg Gibson
A potential application of microbial genetics in forensic science is detection of transfer of the pubic hair microbiome between individuals during sexual intercourse using high-throughput sequencing. In addition to the primary need to show whether the pubic hair microbiome is individualizing, one aspect that must be addressed before using the microbiome in criminal casework involves the impact of storage on the microbiome of samples recovered for forensic testing. To test the effects of short-term storage, pubic hair samples were collected from volunteers and stored at room temperature (∼20°C), refrigerated (4°C), and frozen (-20°C) for 1 week, 2 weeks, 4 weeks, and 6 weeks along with a baseline sample...
October 7, 2016: Forensic Science International. Genetics
Lakshmi Chaitanya, Irena Zupanič Pajnič, Susan Walsh, Jože Balažic, Tomaž Zupanc, Manfred Kayser
Retrieving information about externally visible characteristics from DNA can provide investigative leads to find unknown perpetrators, and can also help in disaster victim and other missing person identification cases. Aiming for the application to both types of forensic casework, we previously developed and forensically validated the HIrisPlex test system enabling parallel DNA prediction of eye and hair colour. Although a recent proof-of-principle study demonstrated the general suitability of the HIrisPlex system for successfully analysing DNA from bones and teeth of various storage times and conditions, practical case applications to human remains are scarce...
October 6, 2016: Forensic Science International. Genetics
Habteab Habtom, Sandrine Demanèche, Lorna Dawson, Chen Azulay, Ofra Matan, Patrick Robe, Ron Gafny, Pascal Simonet, Edouard Jurkevitch, Zohar Pasternak
The ubiquity and transferability of soil makes it a resource for the forensic investigator, as it can provide a link between agents and scenes. However, the information contained in soils, such as chemical compounds, physical particles or biological entities, is seldom used in forensic investigations; due mainly to the associated costs, lack of available expertise, and the lack of soil databases. The microbial DNA in soil is relatively easy to access and analyse, having thus the potential to provide a powerful means for discriminating soil samples or linking them to a common origin...
October 6, 2016: Forensic Science International. Genetics
Michelle Breathnach, Linda Williams, Louise McKenna, Elizabeth Moore
No abstract text is available yet for this article.
October 3, 2016: Forensic Science International. Genetics
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