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Forensic Science International. Genetics

Svitlana V Serga, Ivan V Dombrovskyi, Oleksandr M Maistrenko, Lyudmyla I Ostapchenko, Sergiy V Demydov, Ruslan G Krivda, Iryna A Kozeretska
No abstract text is available yet for this article.
May 10, 2017: Forensic Science International. Genetics
Andrzej Ossowski, Marta Diepenbroek, Maria Szargut, Grażyna Zielińska, Maciej Jędrzejczyk, Jarosław Berent, Renata Jacewicz
No abstract text is available yet for this article.
May 10, 2017: Forensic Science International. Genetics
J Zander, J Rothe, J Dapprich, M Nagy
A particularly challenging aspect of forensic casework is the accurate interpretation of samples that contain DNA from more than one contributor, since DNA analysis of specific identification markers often results in ambiguous mixed profiles. Physically separating the various haploid components of a diploid or polyploid DNA sample or of a specimen containing DNA from multiple individuals can resolve this problem. We adapted and combined previously developed methods of haplotype-specific extraction (HSE) to separate and analyze mitochondrial DNA (mtDNA) in samples containing DNA from two individuals...
May 6, 2017: Forensic Science International. Genetics
Filipa Moreira, João Carneiro, Filipe Pereira
No abstract text is available yet for this article.
May 2, 2017: Forensic Science International. Genetics
F Fontana, C Rapone, G Bregola, R Aversa, A de Meo, G Signorini, M Sergio, A Ferrarini, R Lanzellotto, G Medoro, G Giorgini, N Manaresi, A Berti
Latest genotyping technologies allow to achieve a reliable genetic profile for the offender identification even from extremely minute biological evidence. The ultimate challenge occurs when genetic profiles need to be retrieved from a mixture, which is composed of biological material from two or more individuals. In this case, DNA profiling will often result in a complex genetic profile, which is then subject matter for statistical analysis. In principle, when more individuals contribute to a mixture with different biological fluids, their single genetic profiles can be obtained by separating the distinct cell types (e...
May 1, 2017: Forensic Science International. Genetics
D Rey-González, M Gelabert-Besada, R Cruz, F Brisighelli, M Lopez-Soto, M Rasool, M I Naseer, P Sánchez-Diz, A Carracedo
In this study, we intend to identify the evolutionary footprints of the South Iberian population focusing on the Berber and Arab influence, which has received little attention in the literature. Analysis of the Y-chromosome variation represents a convenient way to assess the genetic contribution of North African populations to the present-day South Iberian genetic pool and could help to reconstruct other demographic events that could have influenced on that region. A total of 26 Y-SNPs and 17 Y-STRs were genotyped in 144 samples from 26 different districts of South Iberia in order to assess the male genetic composition and the level of substructure of male lineages in this area...
May 1, 2017: Forensic Science International. Genetics
Sigal Berlyne, Carla Oz, Naftaly Einot, Shlomit Avraham, Tanya Ram, Miri D Goldberg, Ron Gafny
In 2012 the Israel Police DNA Casework laboratory adopted the 16 STR PowerPlex(®) ESI kit for routine use. The Promega Company updated this kit and developed the PowerPlex(®) ESI 16 Fast System in which all autosomal primer pairs remained identical to the original set, except at the amelogenin site. The master mix was improved and optimized which allowed for direct, faster and more robust amplification. Prior to implementing the PowerPlex(®) ESI 16 Fast System in our lab, we conducted a preliminary assay where 213 casework samples were amplified using the new kit...
April 29, 2017: Forensic Science International. Genetics
Sohee Cho, Sang-Eun Jung, Sae Rom Hong, Eun Hee Lee, Ji Hyun Lee, Soong Deok Lee, Hwan Young Lee
Numerous molecular biomarkers have been proposed as predictors of chronological age. Among them, T-cell specific DNA rearrangement and DNA methylation markers have been introduced as forensic age predictors in blood because of their high prediction accuracy. These markers appear highly promising, but for better application to forensic casework sample analysis the proposed markers and genotyping methods must be tested further. In the current study, signal-joint T-cell receptor excision circles (sjTRECs) and DNA methylation markers located in the ELOVL2, C1orf132, TRIM59, KLF14, and FHL2 genes were reanalyzed in 100 Korean blood samples to test their associations with chronological age, using the same analysis platform used in previous reports...
April 28, 2017: Forensic Science International. Genetics
Thore Egeland, Nádia Pinto, António Amorim
Some practical and theoretical aspects of evaluation of evidence based on the likelihood ratio (LR) in kinship cases are discussed. If relationships are complex or if complicating factors like mutation, correction for population structure or silent alleles need to be accounted for, available software may fail. We present an explicit general formula for non-inbred pairwise cases. Equipped with this formula it is possible to evaluate, say, how strongly a shared rare allele, points towards a specific relationship...
April 27, 2017: Forensic Science International. Genetics
Antonio Alonso, Petra Müller, Lutz Roewer, Sascha Willuweit, Bruce Budowle, Walther Parson
No abstract text is available yet for this article.
April 26, 2017: Forensic Science International. Genetics
Benito Ramos-González, José Alonso Aguilar-Velázquez, María de Lourdes Chávez-Briones, María Del Rocío Escareño-Hernández, Elizabeth Alfaro-Lopez, Héctor Rangel-Villalobos
No abstract text is available yet for this article.
April 23, 2017: Forensic Science International. Genetics
C Phillips
In preparation for the growing use of massively parallel sequencing (MPS) technology to genotype forensic STRs, a comprehensive genomic audit of 73 STRs was made in 2016 [Parson et al., Forensic Sci. Int. Genet. 22, 54-63]. The loci examined included miniSTRs that were not in widespread use, but had been incorporated into MPS kits or were under consideration for this purpose. The current study expands the genomic analysis of autosomal STRs that are not commonly used, to include the full set of developed miniSTRs and an additional 24 STRs, most of which have been recently included in several supplementary forensic multiplex kits for capillary electrophoresis...
April 23, 2017: Forensic Science International. Genetics
Xiaoliang Fu, Jing He, Juanjuan Guo, Yanfang Liu, Zhihui Wang, Jie Lv, Jifeng Cai, Ying Liu, Lagabaiyila Zha
No abstract text is available yet for this article.
April 21, 2017: Forensic Science International. Genetics
Fei Guo
No abstract text is available yet for this article.
April 20, 2017: Forensic Science International. Genetics
Zheng Wang, Weian Du, Guanglin He, Jing Liu, Yiping Hou
Currently, the largest national database within the Y chromosome haplotype reference database (YHRD,, release 53) is China, which has approximately 38000 Y chromosomal 17-marker (Yfiler) haplotypes. These haplotype profiles derived from the vast majority of Chinese administrative divisions, but no haplotype data was available for Hubei province, which is located in the Central China region. Herein, 429 unrelated male Chinese Han individuals residing in Hubei province were recruited and genotyped with 17 Y-STR loci...
April 20, 2017: Forensic Science International. Genetics
Athina Vidaki, Cecilia Johansson, Federica Giangasparo
DNA methylation patterns have the ability to reveal the activities of genes within a certain tissue at a particular time point. Tissue-specific DNA methylation patterns have been previously investigated for their applicability in the identification of forensically relevant body fluids, however there is still a lack in robust markers. While following a genome-wide scale investigation has a great potential to reveal useful tissue-specific changes, a gene-targeted approach can also lead to significant outcomes, especially in genomic locations not included in the genome-wide experiments...
April 19, 2017: Forensic Science International. Genetics
Andrew Loftus, Gina Murphy, Hiromi Brown, Anne Montgomery, Jonathan Tabak, James Baus, Marion Carroll, André Green, Suresh Sikka, Sudhir Sinha
The development and validation of InnoQuant(®) HY, a real-time PCR system containing four DNA targets-two RE autosomal targets of different sizes, male specific targets, and an internal positive control target-are described herein. The ratio of the two autosomal targets provides a Degradation Index, or a quantitative value of a sample's degradation state. The male specific targets are multi-copy targets located on the Y chromosome, which provides information about a sample's male DNA composition. The experimental results demonstrate InnoQuant HY as a robust qPCR method producing accurate DNA quantitation results even at low dynamic ranges, with reproducibility among population groups...
April 17, 2017: Forensic Science International. Genetics
Sarah Riman, Kevin M Kiesler, Lisa A Borsuk, Peter M Vallone
Standard Reference Materials SRM 2392 and 2392-I are intended to provide quality control when amplifying and sequencing human mitochondrial genome sequences. The National Institute of Standards and Technology (NIST) offers these SRMs to laboratories performing DNA-based forensic human identification, molecular diagnosis of mitochondrial diseases, mutation detection, evolutionary anthropology, and genetic genealogy. The entire mtGenome (∼16569bp) of SRM 2392 and 2392-I have previously been characterized at NIST by Sanger sequencing...
April 15, 2017: Forensic Science International. Genetics
M de la Puente, C Phillips, M Fondevila, M Gelabert-Besada, Á Carracedo, M V Lareu
Pentameric-repeat short tandem repeats (STRs), consisting of loci with repeat units of five base-pairs, have the advantage of reduced stutter products compared to their tetrameric-repeat STR counterparts. This characteristic potentially helps the interpretation of mixed DNA profiles when minor component alleles may coincide with stutter peaks from the major components. To develop a simple but informative forensic multiplex with the capability to aid mixture interpretation, we designed an 11-plex assay of nine pentameric STRs new to forensic analysis plus two male- specific markers: DYS391 and the Y-Indel rs2032678 used in GlobalFiler™ (Life Technologies)...
April 15, 2017: Forensic Science International. Genetics
Corina C G Benschop, Linda van de Merwe, Jeroen de Jong, Vanessa Vanvooren, Morgane Kempenaers, C P Kees van der Beek, Filippo Barni, Eusebio López Reyes, Léa Moulin, Laurent Pene, Hinda Haned, Titia Sijen
Searching a national DNA database with complex and incomplete profiles usually yields very large numbers of possible matches that can present many candidate suspects to be further investigated by the forensic scientist and/or police. Current practice in most forensic laboratories consists of ordering these 'hits' based on the number of matching alleles with the searched profile. Thus, candidate profiles that share the same number of matching alleles are not differentiated and due to the lack of other ranking criteria for the candidate list it may be difficult to discern a true match from the false positives or notice that all candidates are in fact false positives...
April 15, 2017: Forensic Science International. Genetics
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