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Forensic Science International. Genetics

Katherine Butler Gettings, Lisa A Borsuk, David Ballard, Martin Bodner, Bruce Budowle, Laurence Devesse, Jonathan King, Walther Parson, Christopher Phillips, Peter M Vallone
The STR Sequencing Project (STRSeq) was initiated to facilitate the description of sequence-based alleles at the Short Tandem Repeat (STR) loci targeted in human identification assays. This international collaborative effort, which has been endorsed by the ISFG DNA Commission, provides a framework for communication among laboratories. The initial data used to populate the project are the aggregate alleles observed in targeted sequencing studies across four laboratories: National Institute of Standards and Technology (N=1786), Kings College London (N=1043), University of North Texas Health Sciences Center (N=839), and University of Santiago de Compostela (N=944), for a total of 4612 individuals...
September 1, 2017: Forensic Science International. Genetics
Iva Gomes, Plácido J P Pereira, Sonja Harms, Andréa M Oliveira, Peter M Schneider, António Brehm
A male West African sample from Guinea-Bissau (West-African coast) was genetically analyzed using 12 X chromosomal short tandem repeats that are grouped into four haplotype groups. Linkage disequilibrium was tested (p≤0.0008) and association was detected for the majority of markers in three out of the four studied haplotype clusters. The sample of 332 unrelated individuals analyzed in this study belonged to several recognized ethnic groups (n=18) which were used to evaluate the genetic variation of Guinea-Bissau's population...
August 24, 2017: Forensic Science International. Genetics
Kristy Steensma, Ricky Ansell, Lindy Clarisse, Edward Connolly, Ate D Kloosterman, Louise G McKenna, Roland A H van Oorschot, Bianca Szkuta, Bas Kokshoorn
To address questions on the activity that led to the deposition of biological traces in a particular case, general information on the probabilities of transfer, persistence and recovery of cellular material in relevant scenarios is necessary. These figures may be derived from experimental data described in forensic literature when conditions relevant to the case were included. The experimental methodology regarding sampling, DNA extraction, DNA typing and profile interpretation that were used to generate these published data may differ from those applied in the case and thus the applicability of the literature data may be questioned...
August 19, 2017: Forensic Science International. Genetics
Athina Vidaki, Celia Díez López, Elena Carnero-Montoro, Arwin Ralf, Kirsten Ward, Timothy Spector, Jordana T Bell, Manfred Kayser
Monozygotic (MZ) twins share the same STR profile, demonstrating a practical problem in forensic casework. DNA methylation has provided a suitable resource for MZ twin differentiation; however, studies addressing the forensic feasibility are lacking. Here, we investigated epigenetic MZ twin differentiation from blood under the forensic scenario comprising i) the discovery of candidate markers in reference-type blood DNA via genome-wide analysis, ii) the technical validation of candidate markers in reference-type blood DNA using a suitable targeted method, and iii) the analysis of the validated markers in trace-type DNA...
August 18, 2017: Forensic Science International. Genetics
Jo-Anne Bright, Duncan Taylor, Simone Gittelson, John Buckleton
No abstract text is available yet for this article.
August 14, 2017: Forensic Science International. Genetics
Yu Tan, Li Wang, Hui Wang, Huan Tian, Zhilong Li, Qian Wang, Hui Jian, Shuqiang Cao, Weibo Liang, Lin Zhang
The resolution of DNA mixtures is still a difficult problem that is worthy of further study. A common method applied for analysing mixtures is the use of autosomal STR markers as well as related calculation software based on genotypes; however, these markers have a limitation in detecting minor DNA in unbalanced mixtures if major DNA constitutes over 95% of the stain. Novel biomarkers, such as Y-STR, DIP-STR and SNP-STR, have been shown to perform well in distinguishing DNA donors in this type of mixture. DIP-STR can successfully target minor DNA in 1000-fold background DNA using two separate allele-specific primers...
August 14, 2017: Forensic Science International. Genetics
Roberta Santangelo, Fabricio González-Andrade, Claus Børsting, Antonio Torroni, Vania Pereira, Niels Morling
Ancestry inference is traditionally done using autosomal SNPs that present great allele frequency differences among populations from different geographic regions. These ancestry informative markers (AIMs) are useful for determining the most likely biogeographic ancestry or population of origin of an individual. Due to the growing interest in AIMs and their applicability in different fields, commercial companies have started to develop AIM multiplexes targeted for Massive Parallel Sequencing platforms. This project focused on the study of three main ethnic groups from Ecuador (Kichwa, Mestizo, and Afro-Ecuadorian) using the Precision ID Ancestry panel (Thermo Fisher Scientific)...
August 14, 2017: Forensic Science International. Genetics
Carolyn R Steffen, Michael D Coble, Katherine B Gettings, Peter M Vallone
No abstract text is available yet for this article.
August 12, 2017: Forensic Science International. Genetics
Daniel Kling, Thore Egeland, Mariana Herrera Piñero, Magnus Dehli Vigeland
Methods and implementations of DNA-based identification are well established in several forensic contexts. However, assessing the statistical power of these methods has been largely overlooked, except in the simplest cases. In this paper we outline general methods for such power evaluation, and apply them to a large set of family reunification cases, where the objective is to decide whether a person of interest (POI) is identical to the missing person (MP) in a family, based on the DNA profile of the POI and available family members...
August 12, 2017: Forensic Science International. Genetics
Xiao-Na Li, Ji-Long Zheng, Yao Jun
No abstract text is available yet for this article.
August 12, 2017: Forensic Science International. Genetics
Kelly A Meiklejohn, James M Robertson
In cases where only a partial or incomplete STR profile is obtained from a sample, information contained in single nucleotide polymorphisms (SNPs) can prove informative for human identification. Thermo Fisher Scientific, which developed the high throughput Ion Torrent(™) PGM(™) sequencer, released the Precision ID Identity Panel, a multiplex SNP panel for human identity. We evaluated the reproducibility and sensitivity of this multiplex, which contains primers for the amplification of 90 autosomal SNPs and 34 Y-clade SNPs...
August 9, 2017: Forensic Science International. Genetics
Mitsuyo Machida, Takashi Taki, Kazuhiko Kibayashi
Short tandem repeat (STR) analysis is generally used for human identification of forensic samples; however, standard STR analysis sometimes fails to generate full profiles since DNA is frequently degraded by various environmental factors. Recently, single nucleotide polymorphism (SNP) analysis has attracted attention for human identification since the shorter amplicons are better suited for degraded samples. Though various SNP loci are used for analysis of degraded samples, it is unclear which ones are more appropriate...
August 9, 2017: Forensic Science International. Genetics
Lijuan Sun, Ke Shi, Lu Tan, Qian Zhang, Lihong Fu, Xiaojing Zhang, Guangping Fu, Shujin Li, Bin Cong
No abstract text is available yet for this article.
August 9, 2017: Forensic Science International. Genetics
Sarah F Bailey, Melissa K Scheible, Christopher Williams, Deborah S B S Silva, Marina Hoggan, Christopher Eichman, Seth A Faith
Next-generation Sequencing (NGS) is a rapidly evolving technology with demonstrated benefits for forensic genetic applications, and the strategies to analyze and manage the massive NGS datasets are currently in development. Here, the computing, data storage, connectivity, and security resources of the Cloud were evaluated as a model for forensic laboratory systems that produce NGS data. A complete front-to-end Cloud system was developed to upload, process, and interpret raw NGS data using a web browser dashboard...
August 8, 2017: Forensic Science International. Genetics
Hussain Alghanim, Joana Antunes, Deborah Soares Bispo Santos Silva, Clarice Sampaio Alho, Kuppareddi Balamurugan, Bruce McCord
Recent developments in the analysis of epigenetic DNA methylation patterns have demonstrated that certain genetic loci show a linear correlation with chronological age. It is the goal of this study to identify a new set of epigenetic methylation markers for the forensic estimation of human age. A total number of 27 CpG sites at three genetic loci, SCGN, DLX5 and KLF14, were examined to evaluate the correlation of their methylation status with age. These sites were evaluated using 72 blood samples and 91 saliva samples collected from volunteers with ages ranging from 5 to 73 years...
August 7, 2017: Forensic Science International. Genetics
Fei Guo
No abstract text is available yet for this article.
August 7, 2017: Forensic Science International. Genetics
Greg Hampikian, Gianluca Peri, Shih-Shiang Lo, Mong-Hwa Chin, Kuo-Lan Liu
We report the case of a suspect (Suspect-3) who was convicted (and later exonerated) of participating in the multiple-attacker rape of two women. The forensic evidence against him was his inclusion in a 17-marker Y-STR mixture isolated from semen on one victim's clothing. The DNA inclusion produced a match statistic with a combined probability of inclusion of 1 in 741, and a Likelihood Ratio of 3296. While the defense team was told that Suspect-3 was included in the semen DNA mixture, they were not told that all of the Y-STR alleles could also be explained by just the other two accused attackers' haplotypes...
August 7, 2017: Forensic Science International. Genetics
Jing Zhang, Junfang Wang, Yaju Liu, Meisen Shi, Rufeng Bai, Shuhua Ma
No abstract text is available yet for this article.
August 5, 2017: Forensic Science International. Genetics
Jana Naue, Huub C J Hoefsloot, Olaf R F Mook, Laura Rijlaarsdam-Hoekstra, Marloes C H van der Zwalm, Peter Henneman, Ate D Kloosterman, Pernette J Verschure
The use of DNA methylation (DNAm) to obtain additional information in forensic investigations showed to be a promising and increasing field of interest. Prediction of the chronological age based on age-dependent changes in the DNAm of specific CpG sites within the genome is one such potential application. Here we present an age-prediction tool for whole blood based on massive parallel sequencing (MPS) and a random forest machine learning algorithm. MPS allows accurate DNAm determination of pre-selected markers and neighboring CpG-sites to identify the best age-predictive markers for the age-prediction tool...
August 1, 2017: Forensic Science International. Genetics
Dragana Zgonjanin, Rashed Alghafri, Mirjana Antov, Goran Stojiljković, Stojan Petković, Radenko Vuković, Dragan Drašković
No abstract text is available yet for this article.
July 29, 2017: Forensic Science International. Genetics
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