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Forensic Science International. Genetics

Xiufeng Zhang, Tao Gu, Jinyong Yao, Canming Yang, Lei Du, Jing Bo Pang, Min Rao, Aiting Nie, Liping Hu, Shengjie Nie
In the present study, 24 Y-chromosomal short tandem repeat (Y-STR) loci were analyzed in 252 unrelated Miao male individuals from Pingbian county, Honghe Hani and Yi Autonomous Prefecture, Yunnan province, southwestern China. The gene diversity of the 24 Y-STR loci in the studied group ranged from 0.2683 (DYS391) to 0.9312 (DYS527a/b). According to haplotypic analysis of the 24 Y-STR loci, 214 different haplotypes were obtained, 186 of which were unique. The overall haplotype diversity and discrimination capacity were calculated to be 0...
February 14, 2017: Forensic Science International. Genetics
Xiufeng Zhang, Liping Hu, Lei Du, Aiting Nie, Min Rao, Jing Bo Pang, Zeng Xiran, Shengjie Nie
The genetic polymorphisms of 20 autosomal short tandem repeat (STR) loci included in the PowerPlex(®) 21 kit were evaluated from 748 unrelated healthy individuals of the Miao ethnic minority living in the Yunnan province in southwestern China. All of the loci reached Hardy-Weinberg equilibrium. These loci were examined to determine allele frequencies and forensic statistical parameters. The genetic relationship between the Miao population and other Chinese populations were also estimated. The combined discrimination power and probability of excluding paternity of the 20 STR loci were 0...
February 6, 2017: Forensic Science International. Genetics
Mitchell M Holland, Erica D Pack, Jennifer A McElhoe
Existing software has not allowed for effective alignment of mitochondrial (mt) DNA sequence data generated using a massively parallel sequencing (MPS) approach, combined with the ability to perform a detailed assessment of the data. The regions of sequence that are typically difficult to align are homopolymeric stretches, isolated patterns of SNPs (single nucleotide polymorphisms), and INDELs (insertions/deletions). A custom software solution, GeneMarker(®) HTS, was developed and evaluated to address these limitations, and to provide a user-friendly interface for forensic practitioners and others interested in mtDNA analysis of MPS data...
February 6, 2017: Forensic Science International. Genetics
Eva Sauer, Antje Extra, Philipp Cachée, Cornelius Courts
The identification of organ tissues in traces recovered from scenes and objects with regard to violent crimes involving serious injuries can be of considerable relevance in forensic investigations. Molecular genetic approaches are provably superior to histological and immunological assays in characterizing organ tissues, and micro-RNAs (miRNAs), due to their cell type specific expression patterns and stability against degradation, emerged as a promising molecular species for forensic analyses, with a range of tried and tested indicative markers...
February 5, 2017: Forensic Science International. Genetics
Søren B Vilsen, Torben Tvedebrink, Helle Smidt Mogensen, Niels Morling
We investigated the results of short tandem repeat (STR) markers of dilution series experiments and reference profiles generated using the Ion PGM massively parallel sequencing platform utilising the HID STR 10-plex panel. The STR markers were identified by the marker specific flanking regions of the STR region. We investigated the following: (1) the usage of quality measures for identifying substitution errors, (2) the heterozygote balance and compared it to that of capillary electrophoresis (CE), (3) the stability of the coverage and the consequence of IonExpress Barcode adapter (IBA) sampling with decreasing amounts of template DNA, (4) the hypothesis that the parental longest uninterrupted stretch (LUS) is a better linear predictor of stutter ratio than the parent allele length, (5) the use of parental allele length as a predictor of shoulder ratio, and (6) the removal of non-systematic erroneous sequences using dynamic thresholds created by fitting the distribution of the non-systematic erroneous sequences...
February 3, 2017: Forensic Science International. Genetics
Junping Han, Jing Sun, Lei Zhao, Wenting Zhao, Yao Liu, Caixia Li
Conventional PCR amplification requires approximately 3h to complete and thus does not meet the requirements of rapid DNA analysis. The purpose of this study was to validate a rapid 15-plex PCR system that can amplify 14 autosomal short tandem repeat (STR) loci (i.e., D6S1043, D21S11, D7S820, CFS1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51, and FGA) and Amelogenin. This system was validated by sensitivity, species specificity, inhibitor tests, sizing accuracy, stutter calculation, concordance tests, DNA mixture, and case sample tests according to the Scientific Working Group for DNA Analysis Methods (SWGDAM) guidelines and Chinese criteria...
February 3, 2017: Forensic Science International. Genetics
Qi Zhao, Yingnan Bian, Suhua Zhang, Ruxin Zhu, Wei Zhou, Yuzhen Gao, Chengtao Li
No abstract text is available yet for this article.
February 1, 2017: Forensic Science International. Genetics
Chunmei Shen, Hongdan Wang, Zhanqi Feng, Qian Dong, Yuxin Guo, Xinxin Wang, Haotian Meng, Ruilin Ma, Jiangwei Yan, Bofeng Zhu, Fadao Tai
No abstract text is available yet for this article.
January 29, 2017: Forensic Science International. Genetics
Magdalena Spólnicka, Joanna Dąbrowska, Emilia Szabłowska-Gnap, Anna Pałeczka, Magdalena Jabłońska, Renata Zbieć-Piekarska, Agnieszka Pięta, Michał Boroń, Magdalena Konarzewska, Grażyna Kostrzewa, Rafał Płoski, Urszula Rogalla, Marcin Woźniak, Tomasz Grzybowski
No abstract text is available yet for this article.
January 27, 2017: Forensic Science International. Genetics
Anne C Jäger, Michelle L Alvarez, Carey P Davis, Ernesto Guzmán, Yonmee Han, Lisa Way, Paulina Walichiewicz, David Silva, Nguyen Pham, Glorianna Caves, Jocelyne Bruand, Felix Schlesinger, Stephanie J K Pond, Joe Varlaro, Kathryn M Stephens, Cydne L Holt
Human DNA profiling using PCR at polymorphic short tandem repeat (STR) loci followed by capillary electrophoresis (CE) size separation and length-based allele typing has been the standard in the forensic community for over 20 years. Over the last decade, Next-Generation Sequencing (NGS) matured rapidly, bringing modern advantages to forensic DNA analysis. The MiSeq FGx™ Forensic Genomics System, comprised of the ForenSeq™ DNA Signature Prep Kit, MiSeq FGx™ Reagent Kit, MiSeq FGx™ instrument and ForenSeq™ Universal Analysis Software, uses PCR to simultaneously amplify up to 231 forensic loci in a single multiplex reaction...
January 27, 2017: Forensic Science International. Genetics
M de la Puente, C Phillips, C Santos, M Fondevila, Á Carracedo, M V Lareu
A new forensic 140-SNP genotyping system from Qiagen, designed for massively parallel sequencing (MPS) analysis, was evaluated using the Ion PGM™ MPS system. Assessments consisted of the sequencing of: established control DNAs that had been previously genotyped with alternative PCR and library preparation kits supplied by Thermo Fisher Scientific for the Ion PGM™ system; a simple set of artificial DNA mixtures; DNA extracted from a degraded femur; and a dilution series to gauge forensic sensitivity. In addition to the reagents for the DNA target capture PCR and library preparation, Qiagen offer an alternative sequence analysis software system (Workbench), which was assessed alongside the Ion PGM™ Genotyper software for forensic MPS analysis...
January 27, 2017: Forensic Science International. Genetics
Jingyan Ji, Zheng Ren, Hongling Zhang, Qiyan Wang, Jie Wang, Zhiming Kong, Chunmei Xu, Mi Tian, Jiang Huang
No abstract text is available yet for this article.
January 25, 2017: Forensic Science International. Genetics
Ian W Evett
No abstract text is available yet for this article.
January 25, 2017: Forensic Science International. Genetics
Smilja Teodorović, Dragan Mijović, Una Radovanović Nenadić, Marina Savić
Worldwide, the establishment of national forensic DNA databases has transformed personal identification in the criminal justice system over the past two decades. It has also stimulated much debate centering on ethical issues, human rights, individual privacy, lack of safeguards and other standards. Therefore, a balance between effectiveness and intrusiveness of a national DNA repository is an imperative and needs to be achieved through a suitable legal framework. On its path to the European Union (EU), the Republic of Serbia is required to harmonize its national policies and legislation with the EU...
January 21, 2017: Forensic Science International. Genetics
Peng Chen, Jing Zhu, Yan Pu, Youjing Jiang, Dan Chen, Hui Wang, Jiong Mao, Bin Zhou, Linbo Gao, Peng Bai, Weibo Liang, Lin Zhang
The recently introduced concept of microhaplotype loci has attracted attention in forensics. Previous studies estimated the allele frequencies generally through obtaining genotypic data on the individual SNPs from a larger set of unrelated individuals then phasing microhaplotypes by statistical and computational techniques. Determining phase for a single new individual requires the larger set of individuals to have been genotyped previously. Rare microhaplotypes possessed only by the target individual or microhaplotypes private to a specific population not previously studied are unlikely to be accurately phased using data sets of SNPs...
January 20, 2017: Forensic Science International. Genetics
Susana Salceda, Arnaldo Barican, Jacklyn Buscaino, Bruce Goldman, Jim Klevenberg, Melissa Kuhn, Dennis Lehto, Frank Lin, Phong Nguyen, Charles Park, Francesca Pearson, Rick Pittaro, Sayali Salodkar, Robert Schueren, Corey Smith, Charles Troup, Dean Tsou, Mattias Vangbo, Justus Wunderle, David King
The RapidHIT(®) ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)-compatible DNA profiles in less than 90min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK™ (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations...
January 12, 2017: Forensic Science International. Genetics
Jun Yao, Hai-Bing Yu, Jia-Xin Xing, Jin-Feng Xuan, Bao-Jie Wang
No abstract text is available yet for this article.
January 11, 2017: Forensic Science International. Genetics
Bianca Szkuta, Kaye N Ballantyne, Roland A H van Oorschot
During the evaluation of forensic DNA evidence in court proceedings, the emphasis previously placed on the source of the DNA is progressively shifting to the consideration of the activities resulting in its deposition. While direct contact and deposition may be a likely explanation, alternative scenarios involving DNA transfer through a secondary person or medium are important to consider. Here we assessed whether non-self DNA, indirectly transferred via a handshake, could be detected on surfaces contacted by the opposing hand-shaker after 15min, and considered the variables affecting its persistence in subsequent contacts...
January 10, 2017: Forensic Science International. Genetics
Rebecca S Just, Lilliana I Moreno, Jill B Smerick, Jodi A Irwin
Though the utility of next-generation sequencing (NGS) technologies for forensic short tandem repeat (STR) typing has been evident for several years, commercially available assays and software solutions developed specifically to meet forensic needs have only recently become available. One of these, the ForenSeq™ DNA Signature Prep Kit (Illumina, Inc.) sequences 27 autosomal STR (aSTR) and 24 Y chromosome STR (Y-STR) loci (concurrent with additional nuclear markers) per multiplexed sample, with automated secondary and tertiary analyses of the data accomplished via the associated ForenSeq™ Universal Analysis Software (UAS)...
January 9, 2017: Forensic Science International. Genetics
Eirik Nataas Hanssen, Robert Lyle, Thore Egeland, Peter Gill
Routine forensic analysis using STRs will fail if the DNA is too degraded. The DNA degradation process in biological stain material is not well understood. In this study we sequenced old semen and blood stains by massively parallel sequencing. The sequence data coverage was used to measure degradation across the genome. The results supported the contention that degradation is uniform across the genome, showing no evidence of regions with increased or decreased resistance towards degradation. Thus the lack of genetic regions robust to degradation removes the possibility of using such regions to further optimize analysis performance for degraded DNA...
January 3, 2017: Forensic Science International. Genetics
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