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Forensic Science International. Genetics

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https://www.readbyqxmd.com/read/28633070/length-and-repeat-sequence-variation-in-58-strs-and-94-snps-in-two-spanish-populations
#1
Ferran Casals, Roger Anglada, Núria Bonet, Raquel Rasal, Kristiaan J van der Gaag, Jerry Hoogenboom, Neus Solé-Morata, David Comas, Francesc Calafell
We have genotyped the 58 STRs (27 autosomal, 24 Y-STRs and 7 X-STRs) and 94 autosomal SNPs in Illumina ForenSeq™ Primer Mix A in 88 Spanish Roma (Gypsy) samples and 143 Catalans. Since this platform is based in massive parallel sequencing, we have used simple R scripts to uncover the sequence variation in the repeat region. Thus, we have found, across 58 STRs, 541 length-based alleles, which, after considering repeat-sequence variation, became 804 different alleles. All loci in both populations were in Hardy-Weinberg equilibrium...
June 16, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28633069/mitogenomic-diversity-in-russians-and-poles
#2
Boris Malyarchuk, Andrey Litvinov, Miroslava Derenko, Katarzyna Skonieczna, Tomasz Grzybowski, Aleksandra Grosheva, Yuri Shneider, Sergei Rychkov, Olga Zhukova
Complete mtDNA genome sequencing improves molecular resolution for distinguishing variation between individuals and populations, but there is still deficiency of mitogenomic population data. To overcome this limitation, we used Sanger-based protocol to generate complete mtDNA sequences of 376 Russian individuals from six populations of European part of Russia and 100 Polish individuals from northern Poland. Nearly complete resolution of mtDNA haplotypes was achieved - about 97% of haplotypes were unique both in Russians and Poles, and no haplotypes overlapped between them when indels were considered...
June 15, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28628900/evaluation-of-the-innotyper-%C3%A2-21-genotyping-kit-in-multi-ethnic-populations
#3
Peter Gustav Ristow, Nicole Barnes, Gina Pineda Murphy, Hiromi Brown, Kevin Wesley Cloete, Maria Eugenia D'Amato
We report the findings of the evaluation of the InnoTyper(®) 21 genotyping kit for the use of human identification (HID) and paternity testing in South Africa. This novel forensic kit evaluates 20 retrotransposable elements (AC4027, MLS26, ALU79712, NBC216, NBC106, RG148, NBC13, AC2265, MLS09, AC1141, TARBP, AC2305, HS4.69, NBC51, ACA1766, NBC120, NBC10, NBC102, SB19.12 and NBC148) and the Amelogenin locus for sex determination. The evaluation of the genotyping performance showed no significant spectral pull-up for peak heights between 100 and 30,000 RFUs...
June 7, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28605649/direct-str-typing-from-presumptively-tested-and-untreated-body-fluids
#4
Phuvadol Thanakiatkrai, Krittika Raham, Jintana Pradutkanchana, Sujettana Sotthibandhu, Thitika Kitpipit
Body fluids provide key pieces of information for a forensic investigation. However, sometimes only a small amount of body fluids is found and/or DNA are also degraded by environmental factors at the crime scene. In extreme cases, a forensic analyst may have to decide whether to perform a presumptive test on the stains or proceed straightaway to DNA profiling, which could be wasteful for non-biological stains. Additionally, due to the inefficient DNA extraction process, the amount of DNA may not be enough for STR typing, especially if parts of the evidence had been subjected to presumptive testing...
June 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28645082/local-populations-and-inaccuracies-determining-the-relevant-mitochondrial-haplotype-distributions-for-north-west-european-cats
#5
Monique Wesselink, Stijn Desmyter, Irene Kuiper
Typing of different portions of the feline mitochondrial control region has illustrated pronounced differences in haplotype distributions between cats from the Netherlands and other parts of the world. To gain a better understanding of the haplotype distribution of North West Continental Europe, 605bp of mitochondrial DNA was typed from randomly selected cats from the Netherlands (N=146), Belgium (N=64) and South West Germany (N=128). The genetic differences between these randomly sampled European populations correlate to the geographical distances, with the Dutch and the South West German populations furthest apart and the Belgian population as an intermediate (Fst values 0...
June 4, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28609758/a-selection-guide-for-the-new-generation-6-dye-dna-profiling-systems
#6
Sze-Wah Lin, Christina Li, Stephen C Y Ip
The Federal Bureau of Investigation (FBI) has recently expanded the CODIS core loci from the existing 13 to 20 as a new guideline, and laboratories from the US are required to comply with the new regulation before uploading or conducting identity search in the national database. The expanded CODIS format, which shares all the core loci commonly used in the European countries and the US, not only increases international compatibility, but also reduces the number of adventitious matches, and hence facilitates international law enforcement and counterterrorism endeavours...
June 3, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28605652/screening-and-confirmation-of-microrna-markers-for-distinguishing-between-menstrual-and-peripheral-blood
#7
Zhilong Li, Peng Bai, Duo Peng, Hui Wang, Yadong Guo, Youjing Jiang, Wang He, Huan Tian, Yu Yang, Yuan Huang, Bing Long, Weibo Liang, Lin Zhang
The identification of menstrual blood (MB) and peripheral blood (PB) left at a crime scene is crucial for crime reconstruction, especially in sexual assaults. MicroRNAs (miRNAs), a class of non-protein coding molecules, have been demonstrated to be a viable tool for body fluid identification in forensic casework. Several groups have searched for miRNAs that are specific to different body fluids. Blood has been studied the most extensively. However, menstrual blood was only involved in five studies, and the results confirming the presence of specific miRNAs could not be reproduced in other studies...
June 3, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28605651/fast-str-allele-identification-with-strait-razor-3-0
#8
August E Woerner, Jonathan L King, Bruce Budowle
The short tandem repeat allele identification tool (STRait Razor), a program used to characterize the haplotypes of short tandem repeats (STRs) in massively parallel sequencing (MPS) data, was redesigned. STRait Razor v3.0 performs ∼660× faster allele identification than its previous version (v2s), a speedup that is largely due to a novel indexing strategy used to perform "fuzzy" (approximate) string matching of anchor sequences. Written in a portable compiled language, C++, STRait Razor v3.0 functions on all major operating systems including Microsoft Windows, and it has cross-platform multithreading support...
June 1, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28605650/body-fluid-prediction-from-microbial-patterns-for-forensic-application
#9
Eirik Nataas Hanssen, Ekaterina Avershina, Knut Rudi, Peter Gill, Lars Snipen
The association of a DNA profile with a certain body fluid can be of essential importance in the evaluation of biological evidence. Several alternative methods for body fluid prediction have been proposed to improve the currently used presumptive tests. Most of them measure gene expression. Here we present a novel approach based on microbial taxonomic profiles obtained by standard 16S rRNA gene sequencing. We used saliva deposited on skin as a forensically relevant study model, but the same principle can be applied for predicting other bacteria rich body fluids...
June 1, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28575840/typing-dna-profiles-from-previously-enhanced-fingerprints-using-direct-pcr
#10
Jennifer E L Templeton, Duncan Taylor, Oliva Handt, Adrian Linacre
Fingermarks are a source of human identification both through the ridge patterns and DNA profiling. Typing nuclear STR DNA markers from previously enhanced fingermarks provides an alternative method of utilising the limited fingermark deposit that can be left behind during a criminal act. Dusting with fingerprint powders is a standard method used in classical fingermark enhancement and can affect DNA data. The ability to generate informative DNA profiles from powdered fingerprints using direct PCR swabs was investigated...
May 21, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28544956/dna-commission-of-the-international-society-for-forensic-genetics-isfg-guidelines-on-the-use-of-x-strs-in-kinship-analysis
#11
Andreas O Tillmar, Daniel Kling, John M Butler, Walther Parson, Mechthild Prinz, Peter M Schneider, Thore Egeland, Leonor Gusmão
Forensic genetic laboratories perform an increasing amount of genetic analyses of the X chromosome, in particular to solve complex cases of kinship analysis. For some biological relationships X-chromosomal markers can be more informative than autosomal markers, and there are a large number of markers, methods and databases that have been described for forensic use. Due to their particular mode of inheritance, and their physical location on a single chromosome, some specific considerations are required when estimating the weight of evidence for X-chromosomal marker DNA data...
May 13, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28527837/allele-frequencies-for-15-str-loci-in-the-ukrainian-population
#12
LETTER
Svitlana V Serga, Ivan V Dombrovskyi, Oleksandr M Maistrenko, Lyudmyla I Ostapchenko, Sergiy V Demydov, Ruslan G Krivda, Iryna A Kozeretska
No abstract text is available yet for this article.
May 10, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28522272/population-analysis-and-forensic-evaluation-of-21-autosomal-loci-included-in-globalfiler%C3%A2-pcr-kit-in-poland
#13
LETTER
Andrzej Ossowski, Marta Diepenbroek, Maria Szargut, Grażyna Zielińska, Maciej Jędrzejczyk, Jarosław Berent, Renata Jacewicz
No abstract text is available yet for this article.
May 10, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28535443/independent-validation-of-body-fluid-specific-cpg-markers-and-construction-of-a-robust-multiplex-assay
#14
Hannah Holtkötter, Vanessa Beyer, Kristina Schwender, Alina Glaub, Kristina Schulze Johann, Marianne Schürenkamp, Ursula Sibbing, Sabrina Banken, Peter Wiegand, Heidi Pfeiffer, Lynn Dennany, Marielle Vennemann, Marielle Vennemann
Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood...
May 9, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28514720/new-application-for-haplotype-specific-extraction-separation-of-mitochondrial-dna-mixtures
#15
J Zander, J Rothe, J Dapprich, M Nagy
A particularly challenging aspect of forensic casework is the accurate interpretation of samples that contain DNA from more than one contributor, since DNA analysis of specific identification markers often results in ambiguous mixed profiles. Physically separating the various haploid components of a diploid or polyploid DNA sample or of a specimen containing DNA from multiple individuals can resolve this problem. We adapted and combined previously developed methods of haplotype-specific extraction (HSE) to separate and analyze mitochondrial DNA (mtDNA) in samples containing DNA from two individuals...
May 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28506735/a-proposal-for-standardization-of-transgenic-reference-sequences-used-in-food-forensics
#16
LETTER
Filipa Moreira, João Carneiro, Filipe Pereira
No abstract text is available yet for this article.
May 2, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28511094/isolation-and-genetic-analysis-of-pure-cells-from-forensic-biological-mixtures-the-precision-of-a-digital-approach
#17
F Fontana, C Rapone, G Bregola, R Aversa, A de Meo, G Signorini, M Sergio, A Ferrarini, R Lanzellotto, G Medoro, G Giorgini, N Manaresi, A Berti
Latest genotyping technologies allow to achieve a reliable genetic profile for the offender identification even from extremely minute biological evidence. The ultimate challenge occurs when genetic profiles need to be retrieved from a mixture, which is composed of biological material from two or more individuals. In this case, DNA profiling will often result in a complex genetic profile, which is then subject matter for statistical analysis. In principle, when more individuals contribute to a mixture with different biological fluids, their single genetic profiles can be obtained by separating the distinct cell types (e...
May 1, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28487219/micro-and-macro-geographical-analysis-of-y-chromosome-lineages-in-south-iberia
#18
D Rey-González, M Gelabert-Besada, R Cruz, F Brisighelli, M Lopez-Soto, M Rasool, M I Naseer, P Sánchez-Diz, A Carracedo
In this study, we intend to identify the evolutionary footprints of the South Iberian population focusing on the Berber and Arab influence, which has received little attention in the literature. Analysis of the Y-chromosome variation represents a convenient way to assess the genetic contribution of North African populations to the present-day South Iberian genetic pool and could help to reconstruct other demographic events that could have influenced on that region. A total of 26 Y-SNPs and 17 Y-STRs were genotyped in 144 samples from 26 different districts of South Iberia in order to assess the male genetic composition and the level of substructure of male lineages in this area...
May 1, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28511096/improved-amplification-results-following-episodes-of-failure-to-amplify-at-the-amelogenin-locus-using-powerplex-%C3%A2-esi-16-fast-system
#19
Sigal Berlyne, Carla Oz, Naftaly Einot, Shlomit Avraham, Tanya Ram, Miri D Goldberg, Ron Gafny
In 2012 the Israel Police DNA Casework laboratory adopted the 16 STR PowerPlex(®) ESI kit for routine use. The Promega Company updated this kit and developed the PowerPlex(®) ESI 16 Fast System in which all autosomal primer pairs remained identical to the original set, except at the amelogenin site. The master mix was improved and optimized which allowed for direct, faster and more robust amplification. Prior to implementing the PowerPlex(®) ESI 16 Fast System in our lab, we conducted a preliminary assay where 213 casework samples were amplified using the new kit...
April 29, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28511095/independent-validation-of-dna-based-approaches-for-age-prediction-in-blood
#20
Sohee Cho, Sang-Eun Jung, Sae Rom Hong, Eun Hee Lee, Ji Hyun Lee, Soong Deok Lee, Hwan Young Lee
Numerous molecular biomarkers have been proposed as predictors of chronological age. Among them, T-cell specific DNA rearrangement and DNA methylation markers have been introduced as forensic age predictors in blood because of their high prediction accuracy. These markers appear highly promising, but for better application to forensic casework sample analysis the proposed markers and genotyping methods must be tested further. In the current study, signal-joint T-cell receptor excision circles (sjTRECs) and DNA methylation markers located in the ELOVL2, C1orf132, TRIM59, KLF14, and FHL2 genes were reanalyzed in 100 Korean blood samples to test their associations with chronological age, using the same analysis platform used in previous reports...
April 28, 2017: Forensic Science International. Genetics
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