Read by QxMD icon Read

Forensic Science International. Genetics

María Saiz, Maria Jesus Álvarez-Cubero, Luis Javier Martinez-Gonzalez, Juan Carlos Álvarez, José Antonio Lorente
No abstract text is available yet for this article.
March 8, 2018: Forensic Science International. Genetics
Shao-Kang Mo, Zi-Lin Ren, Ya-Ran Yang, Ya-Cheng Liu, Jing-Jing Zhang, Hui-Juan Wu, Zhen Li, Xiao-Chen Bo, Sheng-Qi Wang, Jiang-Wei Yan, Ming Ni
Kinship testing based on genetic markers, as forensic short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs), has valuable practical applications. Paternity and first-degree relationship can be accurately identified by current commonly-used forensic STRs and reported SNP markers. However, second-degree and more distant relationships remain challenging. Although ∼105 -106 SNPs can be used to estimate relatedness of higher degrees, genome-wide genotyping and analysis may be impractical for forensic use...
March 2, 2018: Forensic Science International. Genetics
Anastasia Aliferi, Jim Thomson, Andrew McDonald, Vanessa Molin Paynter, Steven Ferguson, Des Vanhinsbergh, Denise Syndercombe Court, David Ballard
A total of 3128 Y-STR profiles from three UK and one Irish population have been analysed with the PowerPlex Y23 system and are reported here. Instances of haplotype sharing between apparently unrelated individuals were identified and further investigated with the use of the 5 additional markers within the Yfiler Plus kit, resulting in a reduction by 76% in the number of shared haplotypes. Furthermore, Yfiler Plus was also employed to verify locus deletions and duplications observed in Y23 genotypes while inconsistencies between the two kits were sequenced, revealing underlying Y23 primer binding site mutations in loci DYS392 and DYS576...
March 2, 2018: Forensic Science International. Genetics
Rebecca S Just, Jodi A Irwin
Some of the expected advantages of next generation sequencing (NGS) for short tandem repeat (STR) typing include enhanced mixture detection and genotype resolution via sequence variation among non-homologous alleles of the same length. However, at the same time that NGS methods for forensic DNA typing have advanced in recent years, many caseworking laboratories have implemented or are transitioning to probabilistic genotyping to assist the interpretation of complex autosomal STR typing results. Current probabilistic software programs are designed for length-based data, and were not intended to accommodate sequence strings as the product input...
February 21, 2018: Forensic Science International. Genetics
Mark D Timken, Sonja B Klein, Martin R Buoncristiani
The efficacy of a DNA differential extraction procedure relies on reducing the amount of non-sperm female DNA carryover into the sperm fraction, while providing a sufficient recovery of male DNA from the sperm cell component. A standard approach to this extraction is to use a mild initial lysis step to digest the female (epithelial cell) component in the mixture, followed by a series of centrifugation and wash steps to further purify the resulting sperm-pellet fraction. This sperm fraction is then digested in the presence of a chemical reducing agent in preparation for DNA extraction...
February 21, 2018: Forensic Science International. Genetics
C Phillips, K Butler Gettings, J L King, D Ballard, M Bodner, L Borsuk, W Parson
The STR sequence template file published in 2016 as part of the considerations from the DNA Commission of the International Society for Forensic Genetics on minimal STR sequence nomenclature requirements, has been comprehensively revised and audited using the latest GRCh38 genome assembly. The list of forensic STRs characterized was expanded by including supplementary autosomal, X- and Y-chromosome microsatellites in less common use for routine DNA profiling, but some likely to be adopted in future massively parallel sequencing (MPS) STR panels...
February 21, 2018: Forensic Science International. Genetics
Barbara Karolina Zajac, Richard Zehner, Stefanie Scheiper, Melanie Weissenberger
Throughout the last decade more companies have been offering multiplex PCR kits for forensic STR typing. As a consequence, it has been demonstrated, that an observed genotype may unexpectedly vary at a single locus when different STR kits have been used. Analysing STR profiles which have to be entered in a national database, unknown or undetected primer binding site mutations, insertions or deletions within the flanking region of STR loci may hinder matches and therefore have far-reaching consequences. The current study is a further example indicating that sequence variations in flanking regions are a common problem within STR typing which should not be underestimated...
February 20, 2018: Forensic Science International. Genetics
Hyun-Chul Park, Eu-Ree Ahn, Ju Yeon Jung, Ji-Hye Park, Jee Won Lee, Si-Keun Lim, Won Kim
DNA methylation has important biological roles, such as gene expression regulation, as well as practical applications in forensics, such as in body fluid identification and age estimation. DNA methylation often occurs in the CpG site, and methylation within the CpG islands affects various cellular functions and is related to tissue-specific identification. Several programs have been developed to identify CpG islands; however, the size, location, and number of predicted CpG islands are not identical due to different search algorithms...
February 17, 2018: Forensic Science International. Genetics
Alan M Magee, Michelle Breathnach, Stephen Doak, Fiona Thornton, Conor Noone, Louise G McKenna
During an investigation, the question of interest might be whether or not a person has worn a given garment. Wearer DNA studies have contributed to our knowledge and understanding of DNA transfer and persistence on items of worn clothing. However, there is currently no extensive dataset on DNA profiling outcomes from a variety of upper garments. Therefore we investigated the DNA profiling outcomes from the collars and/or cuffs of forty-four upper garments of worn clothing and determined the quantity of wearer and non-wearer DNA recovered...
February 13, 2018: Forensic Science International. Genetics
Zheng Wang, Guanglin He, Tao Luo, Xueying Zhao, Jing Liu, Mengge Wang, Di Zhou, Xu Chen, Chengtao Li, Yiping Hou
The Tibeto-Burman language, one subfamily of the Sino-Tibetan languages, is spoken by over 60 million people all over East Asia. Yet the ethnic origin and genetic architecture of Tibeto-Burman speaking populations remain largely unexplored. In the present study, 169 Chinese individuals from Tibeto-Burman speaking populations (two ethnic groups: Tibetan and Yi) in four different geographic regions in western China were analyzed using the Precision ID Ancestry Panel (165 AISNPs) and the Ion PGM System. The performance and corresponding forensic statistical parameters of this AISNPs panel were investigated...
February 13, 2018: Forensic Science International. Genetics
Jacklyn Buscaino, Arnaldo Barican, Leto Farrales, Bruce Goldman, Jim Klevenberg, Melissa Kuhn, Frank Lin, Phong Nguyen, Susana Salceda, Robert Schueren, Corey Smith, Charles Troup, Dean Tsou, Mattias Vangbo, David King
The turn-around time of urgent crime scene DNA samples is often far longer than desired by law enforcement. Crime scene DNA sample processing involves both complex and routine processing steps. Simplification and integration of the routine steps would dramatically improve turn-around time and reduce the risk of operator contamination. Routine DNA extraction and quantitation is readily available. However, PCR amplification and electrophoretic analysis remain largely manual. Rapid DNA Analysis is a hands-free "swab in - profile out" process which consists of automated DNA extraction, amplification, separation, detection, and allele calling without human intervention...
February 13, 2018: Forensic Science International. Genetics
Inge Smeers, Ronny Decorte, Wim Van de Voorde, Bram Bekaert
DNA methylation is a promising biomarker for forensic age prediction. A challenge that has emerged in recent studies is the fact that prediction errors become larger with increasing age due to interindividual differences in epigenetic ageing rates. This phenomenon of non-constant variance or heteroscedasticity violates an assumption of the often used method of ordinary least squares (OLS) regression. The aim of this study was to evaluate alternative statistical methods that do take heteroscedasticity into account in order to provide more accurate, age-dependent prediction intervals...
February 9, 2018: Forensic Science International. Genetics
Nicholas Buchanan, Fabian Staubach, Matthias Wienroth, Peter Pfaffelhuber, Mihai Surdu, Anna Lipphardt, Anna Köttgen, Denise Syndercombe-Court, Veronika Lipphardt
No abstract text is available yet for this article.
February 9, 2018: Forensic Science International. Genetics
Magdalena Spólnicka, Renata Zbieć-Piekarska, Marta Karp, Marcin M Machnicki, Paulina Własiuk, Żanetta Makowska, Agnieszka Pięta, Tomasz Gambin, Piotr Gasperowicz, Wojciech Branicki, Krzysztof Giannopoulos, Tomasz Stokłosa, Rafał Płoski
No abstract text is available yet for this article.
February 7, 2018: Forensic Science International. Genetics
C Robino, A Ralf, S Pasino, M R De Marchi, K N Ballantyne, A Barbaro, C Bini, E Carnevali, L Casarino, C Di Gaetano, M Fabbri, G Ferri, E Giardina, A Gonzalez, G Matullo, A L Nutini, V Onofri, A Piccinini, M Piglionica, E Ponzano, C Previderè, N Resta, F Scarnicci, G Seidita, S Sorçaburu-Cigliero, S Turrina, A Verzeletti, M Kayser
No abstract text is available yet for this article.
February 7, 2018: Forensic Science International. Genetics
Filipa Simão, Ana Paula Ferreira, Elizeu Fagundes de Carvalho, Walther Parson, Leonor Gusmão
The genetic composition of the Brazilian population was shaped by interethnic admixture between autochthonous Native Americans, Europeans settlers and African slaves. This structure, characteristic of most American populations, implies the need for large population forensic databases to capture the high diversity that is usually associated with admixed populations. In the present work, we sequenced the control region of mitochondrial DNA from 205 non-related individuals living in the Rio de Janeiro metropolitan region...
February 6, 2018: Forensic Science International. Genetics
Xiaohong Zhao, Xiaogang Chen, Yuancun Zhao, Shu Zhang, Zehua Gao, Yiwen Yang, Yufang Wang, Ji Zhang
Insertion/deletion polymorphisms (indels), which combine the advantages of both short tandem repeats and single-nucleotide polymorphisms, are suitable for parentage testing. To overcome the limitations of the low polymorphism of di-allelic indels, we constructed a set of haplotypes with physically linked, multi-allelic indels. Candidate haplotypes were selected from the 1000 Genomes Project database, and were subject to the following criteria for inclusion: (i) each marker must have a minimum allele frequency (MAF) of ≥0...
February 6, 2018: Forensic Science International. Genetics
Felicia Bardan, Denice Higgins, Jeremy J Austin
Short Tandem Repeat (STR) genotyping is currently the primary DNA-based method for human identification, however it can have limited success when applied to degraded human remains. Massively parallel sequencing (MPS) provides new opportunities to obtain genetic data for hundreds of loci in a single assay with higher success from degraded samples. However, due to the extra requirement for specialised equipment, expertise and resources, routine use of MPS may not be feasible or necessary for many forensic cases...
February 6, 2018: Forensic Science International. Genetics
(no author information available yet)
No abstract text is available yet for this article.
February 5, 2018: Forensic Science International. Genetics
Nikhil Bose, Katie Carlberg, George Sensabaugh, Henry Erlich, Cassandra Calloway
DNA from biological forensic samples can be highly fragmented and present in limited quantity. When DNA is highly fragmented, conventional PCR based Short Tandem Repeat (STR) analysis may fail as primer binding sites may not be present on a single template molecule. Single Nucleotide Polymorphisms (SNPs) can serve as an alternative type of genetic marker for analysis of degraded samples because the targeted variation is a single base. However, conventional PCR based SNP analysis methods still require intact primer binding sites for target amplification...
February 4, 2018: Forensic Science International. Genetics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"