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Stem Cell Research

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https://www.readbyqxmd.com/read/28549249/cd146-mcam-in-human-cs-dlk1-cs-cd34-adipose-stromal-progenitor-cells
#1
Susanne Hörl, Asim Ejaz, Sebastian Ernst, Monika Mattesich, Andreas Kaiser, Brigitte Jenewein, Marit E Zwierzina, Sarina Hammerle, Carina Miggitsch, Maria C Mitterberger-Vogt, Claudia Krautgasser, Gerhard Pierer, Werner Zwerschke
To precisely characterize CD146 in adipose stromal/progenitor cells (ASCs) we sorted the stromal vascular faction (SVF) of human abdominal subcutaneous white adipose tissue (sWAT) according to cell surface (cs) expression of CD146, DLK1 and CD34. This test identified three main SVF cell populations: ~50% cs-DLK1(-)/cs-CD34(+)/cs-CD146(-) ASCs, ~7.5% cs-DLK1(+)/cs-CD34(dim/+)/cs-CD146(+) and ~7.5% cs-DLK1(+)/cs-CD34(dim/+)/cs-CD146(-) cells. All cells contained intracellular CD146. Whole mount fluorescent IHC staining of small vessels detected CD146(+) endothelial cells (CD31(+)/CD34(+)/CD146(+)) and pericytes (CD31(-)/CD34(-)/CD146(+) ASCs)...
May 17, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28500913/myb-fusions-and-cd-markers-as-tools-for-authentication-and-purification-of-cancer-stem-cells-from-salivary-adenoid-cystic-carcinoma
#2
Alex Panaccione, Yi Zhang, Molly Ryan, Christopher A Moskaluk, Karen S Anderson, Wendell G Yarbrough, Sergey V Ivanov
Cancer stem cells (CSC) are considered the major cause of aggressive tumor behavior, recurrence, metastases, and resistance to radiation, making them an attractive therapeutic target. However, isolation of CSC from tumor tissue and their characterization are challenging due to uncertainty about their molecular markers and conditions for their propagation. Adenoid cystic carcinoma (ACC), which arises predominantly in the salivary glands, is a slow-growing but relentless tumor that frequently invades nerves and metastasizes...
May 5, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28494434/p-cadherin-is-necessary-for-retinal-stem-cell-behavior-in-vitro-but-not-in-vivo
#3
Brenda L K Coles, Derek van der Kooy
Adult retinal stem cells (RSCs) are rare quiescent cells within the ciliary epithelium of the eye, which is made up of non-pigmented N-Cadherin(+ve) inner and pigmented P-Cadherin(+ve) outer cell layers. Through FACs and single cell analyses, we have shown that RSCs arise from single cells from within the pigmented CE and express P-Cadherin. However, whether the expression of P-Cadherin is required for maintenance of the stem cell in vivo or in the formation of the clonal stem cell spheres in vitro is not known...
May 3, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28499264/initial-clonogenic-potential-of-human-endothelial-progenitor-cells-is-predictive-of-their-further-properties-and-establishes-a-functional-hierarchy-related-to-immaturity
#4
Ségolène Ferratge, Guillaume Ha, Gilles Carpentier, Nassim Arouche, Rümeyza Bascetin, Laurent Muller, Stéphane Germain, Georges Uzan
Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals...
April 21, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28445828/targeting-the-aryl-hydrocarbon-receptor-nuclear-translocator-complex-with-dmog-and-stemregenin-1-improves-primitive-hematopoietic-stem-cell-expansion
#5
Carlo Stephan Jackson, Chrisna Durandt, Ilse Janse van Rensburg, Vincent Praloran, Philippe Brunet de la Grange, Michael Sean Pepper
Culture conditions used for the expansion of hematopoietic stem and progenitor cells (HSCs and HPCs, collectively HSPCs) should ideally favor the self renewal of long-term HSCs. At 20% O2, the synthesis of HIF-1α is balanced by its hydroxylation and proteasomal degradation. This favors HSPC differentiation, but can be prevented by culturing CD34+ cord blood cells in the presence of dimethyloxaloylglycine (DMOG). This differentiation may also be reduced by culturing the cells in the presence of Stemregenin 1, an antagonist of the aryl hydrocarbon receptor (AhR)...
April 20, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28434825/rna-binding-proteins-in-human-oogenesis-balancing-differentiation-and-self-renewal-in-the-female-fetal-germline
#6
Roseanne Rosario, Andrew J Childs, Richard A Anderson
Primordial germ cells undergo three significant processes on their path to becoming primary oocytes: the initiation of meiosis, the formation and breakdown of germ cell nests, and the assembly of single oocytes into primordial follicles. However at the onset of meiosis, the germ cell becomes transcriptionally silenced. Consequently translational control of pre-stored mRNAs plays a central role in coordinating gene expression throughout the remainder of oogenesis; RNA binding proteins are key to this regulation...
April 18, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28433654/high-molecular-weight-fgf2-isoforms-demonstrate-canonical-receptor-mediated-activity-and-support-human-embryonic-stem-cell-self-renewal
#7
Denis Kole, Alexandra Grella, David Dolivo, Lucia Shumaker, William Hermans, Tanja Dominko
Basic fibroblast growth factor (FGF2) is a highly pleiotropic member of a large family of growth factors with a broad range of activities, including mitogenesis and angiogenesis (Ornitz et al., 1996; Zhang et al., 2006), and it is known to be essential for maintenance of balance between survival, proliferation, and self-renewal in human pluripotent stem cells (Eiselleova et al., 2009; Zoumaro-Djayoon et al., 2011). A single FGF2 transcript can be translated into five FGF2 protein isoforms, an 18kDa low molecular weight (LMW) isoform and four larger high molecular weight (HMW) isoforms (Arese et al...
April 18, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28433655/targeting-sarcoma-tumor-initiating-cells-through-differentiation-therapy
#8
Dan Han, Veronica Rodriguez-Bravo, Elizabeth Charytonowicz, Elizabeth Demicco, Josep Domingo-Domenech, Robert G Maki, Carlos Cordon-Cardo
Human leukocyte antigen class I (HLA-I) down-regulation has been reported in many human cancers to be associated with poor clinical outcome. However, its connection to tumor-initiating cells (TICs) remains unknown. In this study, we report that HLA-I is down-regulated in a subpopulation of cells that have high tumor initiating capacity in different types of human sarcomas. Detailed characterization revealed their distinct molecular profiles regarding proliferation, apoptosis and stemness programs. Notably, these TICs can be induced to differentiate along distinct mesenchymal lineages, including the osteogenic pathway...
April 13, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28431857/modeling-human-infertility-with-pluripotent-stem-cells
#9
Di Chen, Joanna J Gell, Yu Tao, Enrique Sosa, Amander T Clark
Human fertility is dependent upon the correct establishment and differentiation of the germline. This is because no other cell type in the body is capable of passing a genome and epigenome from parent to child. Terminally differentiated germline cells in the adult testis and ovary are called gametes. However, the initial specification of germline cells occurs in the embryo around the time of gastrulation. Most of our knowledge regarding the cell and molecular events that govern human germline specification involves extrapolating scientific principles from model organisms, most notably the mouse...
April 13, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28426972/unlocking-the-chromatin-of-adenoid-cystic-carcinomas-using-hdac-inhibitors-sensitize-cancer-stem-cells-to-cisplatin-and-induces-tumor-senescence
#10
Luciana O Almeida, Douglas M Guimarães, Manoela D Martins, Marco A T Martins, Kristy A Warner, Jacques E Nör, Rogerio M Castilho, Cristiane H Squarize
No abstract text is available yet for this article.
April 8, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28412509/a-monoclonal-antibody-recognizes-undifferentiation-specific-carbohydrate-moieties-expressed-on-cell-surface-of-the-human-dental-pulp-cells
#11
Kyung-Jung Kang, Seon-Yle Ko, Chun-Jeih Ryu, Young-Joo Jang
Human dental pulp cells are obtained from dental pulp tissue, and have the ability to form dentin and a pulp-like complex. Although adult stem cells have been identified from the primary culture by using specific cell surface markers, the identity of surface markers for the purification of stem cells within the dental pulp population are still unclear. Previously, we had constructed monoclonal antibodies against the undifferentiated cell-specific surface markers of human dental pulp cells (hDPCs) by performing decoy immunization...
April 6, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28411439/neuropeptide-y-enhances-proliferation-and-prevents-apoptosis-in-rat-bone-marrow-stromal-cells-in-association-with-activation-of-the-wnt-%C3%AE-catenin-pathway-in-vitro
#12
Jianqun Wu, Song Liu, Huan Meng, Tianyu Qu, Su Fu, Zhao Wang, Jianguo Yang, Dan Jin, Bin Yu
Neuropeptide Y (NPY) exhibits a critical but poorly understood regulatory signaling function and has been shown to promote proliferation, vascularization and migration in several types of cells and tissues. However, little is known about the specific role of NPY in the proliferation and apoptosis of bone marrow stromal cells (also known as bone marrow-derived mesenchymal stem cells, BMSCs), which contain a subpopulation of multipotent skeletal stem cells. Based on BrdU incorporation tests, Cell Counting Kit-8, flow cytometry, quantitative polymerase chain reaction and western blotting, we showed that NPY significantly promoted the proliferation of BMSCs in a concentration-dependent manner, with a maximal effect observed at a concentration of 10(-10)M for pro-proliferative and 10(-12)M for anti-apoptotic activities...
April 5, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28390247/comparative-expression-analysis-of-shox2-deficient-embryonic-stem-cell-derived-sinoatrial-node-like-cells
#13
Sandra Hoffmann, Stefanie Schmitteckert, Anne Griesbeck, Hannes Preiss, Simon Sumer, Alexandra Rolletschek, Martin Granzow, Volker Eckstein, Beate Niesler, Gudrun A Rappold
The homeodomain transcription factor Shox2 controls the development and function of the native cardiac pacemaker, the sinoatrial node (SAN). Moreover, SHOX2 mutations have been associated with cardiac arrhythmias in humans. For detailed examination of Shox2-dependent developmental mechanisms in SAN cells, we established a murine embryonic stem cell (ESC)-based model using Shox2 as a molecular tool. Shox2(+/+) and Shox2(-/-) ESC clones were isolated and differentiated according to five different protocols in order to evaluate the most efficient enrichment of SAN-like cells...
March 29, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28368243/pyrimidoindole-derivative-um171-enhances-derivation-of-hematopoietic-progenitor-cells-from-human-pluripotent-stem-cells
#14
Xuejia Li, Chengxiang Xia, Tongjie Wang, Lijuan Liu, Qianhao Zhao, Dan Yang, Fangxiao Hu, Mengyun Zhang, Ke Huang, Yang Geng, Yi Zheng, Yuxian Guan, Hongling Wu, Xiaoli Chen, Guangjin Pan, Jiekai Chen, Juan Du, Jinyong Wang
In the field of hematopoietic regeneration, deriving hematopoietic stem cells (HSCs) from pluripotent stem cells with engraftment potential is the central mission. Unstable hematopoietic differentiation protocol due to variation factors such as serums and feeder cells, remains a major technical issue impeding the screening of key factors for the derivation of HSCs. In combination with hematopoietic cytokines, UM171 has the capacity to facilitate the maintenance and expansion of human primary HSCs in vitro. Here, using a serum-free, feeder-free, and chemically defined induction protocol, we observed that UM171 enhanced hematopoietic derivation through the entire process of hematopoietic induction in vitro...
March 21, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28390992/patient-specific-induced-pluripotent-stem-cells-to-evaluate-the-pathophysiology-of-trnt1-associated-retinitis-pigmentosa
#15
Tasneem P Sharma, Luke A Wiley, S Scott Whitmore, Kristin R Anfinson, Cathryn M Cranston, Douglas J Oppedal, Heather T Daggett, Robert F Mullins, Budd A Tucker, Edwin M Stone
Retinitis pigmentosa (RP) is a heterogeneous group of monogenic disorders characterized by progressive death of the light-sensing photoreceptor cells of the outer neural retina. We recently identified novel hypomorphic mutations in the tRNA Nucleotidyl Transferase, CCA-Adding 1 (TRNT1) gene that cause early-onset RP. To model this disease in vitro, we generated patient-specific iPSCs and iPSC-derived retinal organoids from dermal fibroblasts of patients with molecularly confirmed TRNT1-associated RP. Pluripotency was confirmed using rt-PCR, immunocytochemistry, and a TaqMan Scorecard Assay...
March 18, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28336253/mitochondrial-matters-mitochondrial-bottlenecks-self-assembling-structures-and-entrapment-in-the-female-germline
#16
Florence L Marlow
Mitochondrial replacement therapy, a procedure to generate embryos with the nuclear genome of a donor mother and the healthy mitochondria of a recipient egg, has recently emerged as a promising strategy to prevent transmission of devastating mitochondrial DNA diseases and infertility. The procedure may produce an embryo that is free of diseased mitochondria. A recent study addresses important fundamental questions about the mechanisms underlying maternal inheritance and translational questions regarding the transgenerational effectiveness of this promising therapeutic strategy...
March 15, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395748/generation-of-induced-pluripotent-stem-cells-from-peripheral-blood-cd34-hematopoietic-progenitors-of-a-31year-old-healthy-woman
#17
Amornrat Tangprasittipap, Bunyada Jittorntrum, Wasinee Wongkummool, Narisorn Kitiyanant, Alisa Tubsuwan
The MUi019-A human induced pluripotent stem cell line was generated from peripheral blood CD34+ hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019-A to model disease while the cell type affected by the disease is inaccessible...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395747/generation-of-induced-pluripotent-stem-cells-ipscs-from-a-hypertrophic-cardiomyopathy-patient-with-the-pathogenic-variant-p-val698ala-in-beta-myosin-heavy-chain-myh7-gene
#18
Samantha Barratt Ross, Stuart T Fraser, Natalie Nowak, Christopher Semsarian
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the whole blood of a 43-year-old male with hypertrophic cardiomyopathy (HCM) who carries the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7). Patient-derived PBMCs were reprogrammed using non-integrative episomal vectors containing reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry the pathogenic MYH7 variant p...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395746/derivation-of-the-human-induced-pluripotent-stem-cell-line-mui017-a-from-a-patient-with-homozygous-hemoglobin-constant-spring
#19
Wasinee Wongkummool, Warun Maneepitasut, Thongperm Munkongdee, Pirut Tong-Ngam, Amornrat Tangprasittipap, Saovaros Svasti, Narisorn Kitiyanant, Kittiphong Paiboonsukwong, Suthat Fucharoen, Alisa Tubsuwan
Hemoglobin Constant Spring (HbCS, HBA2: c.427T>C) is a common nondeletional α-thalassemia resulting from a nucleotide substitution at the termination codon of the HBA2 gene. Homozygosity for HbCS is characterized with mild anemia, jaundice, and splenomegaly. In this study, the human induced pluripotent stem cell line MUi017-A was successfully generated from peripheral blood CD34+ hematopoietic progenitors of a 52year old female with homozygous HbCS. The MUi017-A cell line exhibited embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of differentiating into the three germ layers...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28395745/establishment-of-mui009-a-human-induced-pluripotent-stem-cells-from-a-32year-old-male-with-homozygous-%C3%AE-%C3%A2-thalassemia-coinherited-with-heterozygous-%C3%AE-thalassemia-2
#20
Wasinee Wongkummool, Warun Maneepitasut, Pirut Tong-Ngam, Amornrat Tangprasittipap, Thongperm Munkongdee, Chanikarn Boonchuay, Saovaros Svasti, Narisorn Kitiyanant, Kittiphong Paiboonsukwong, Suthat Fucharoen, Alisa Tubsuwan
The thalassemias are a group of genetic disorders characterized by a deficiency in the synthesis of globin chains. In this study the MUi009-A human induced pluripotent stem cell line was successfully generated from peripheral blood CD34+ haematopoietic progenitors of a 32year old male who had coinherited a homozygous β°-thalassemia mutation at codon 41/42 (-TCTT) and a heterozygous α-thalassemia 4.2 deletion. The MUi009-A cell line exhibited embryonic stem cell characteristics with consistent pluripotency marker expression and the capability of differentiating into the three germ layers...
April 2017: Stem Cell Research
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