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Biotechnology for Biofuels

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https://www.readbyqxmd.com/read/28630648/comparative-metabolic-profiling-of-the-lipid-producing-green-microalga-chlorella-reveals-that-nitrogen-and-carbon-metabolic-pathways-contribute-to-lipid-metabolism
#1
Hui Chen, Yanli Zheng, Jiao Zhan, Chenliu He, Qiang Wang
Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting conditions. However, the underlying mechanisms of lipid biosynthesis are unclear. In this study, we analyzed the global metabolic profiles of three genetically closely related Chlorella strains (C1, C2, and C3) with significant differences in lipid productivity to identify the contributions of key metabolic pathways to lipid metabolism...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616076/regulators-of-plant-biomass-degradation-in-ascomycetous-fungi
#2
REVIEW
Tiziano Benocci, Maria Victoria Aguilar-Pontes, Miaomiao Zhou, Bernhard Seiboth, Ronald P de Vries
Fungi play a major role in the global carbon cycle because of their ability to utilize plant biomass (polysaccharides, proteins, and lignin) as carbon source. Due to the complexity and heterogenic composition of plant biomass, fungi need to produce a broad range of degrading enzymes, matching the composition of (part of) the prevalent substrate. This process is dependent on a network of regulators that not only control the extracellular enzymes that degrade the biomass, but also the metabolic pathways needed to metabolize the resulting monomers...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616075/proximity-ligation-scaffolding-and-comparison-of-two-trichoderma-reesei-strains-genomes
#3
Etienne Jourdier, Lyam Baudry, Dante Poggi-Parodi, Yoan Vicq, Romain Koszul, Antoine Margeot, Martial Marbouty, Frédérique Bidard
BACKGROUND: The presence of low complexity and repeated regions in genomes often results in difficulties to assemble sequencing data into full chromosomes. However, the availability of full genome scaffolds is essential to several investigations, regarding for instance the evolution of entire clades, the analysis of chromosome rearrangements, and is pivotal to sexual crossing studies. In non-conventional but industrially relevant model organisms, such as the ascomycete Trichoderma reesei, a complete genome assembly is seldom available...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616074/thermodynamics-and-economic-feasibility-of-acetone-production-from-syngas-using-the-thermophilic-production-host-moorella-thermoacetica
#4
Stephanie Redl, Sumesh Sukumara, Tom Ploeger, Liang Wu, Torbjørn Ølshøj Jensen, Alex Toftgaard Nielsen, Henk Noorman
BACKGROUND: Syngas fermentation is a promising option for the production of biocommodities due to its abundance and compatibility with anaerobic fermentation. Using thermophilic production strains in a syngas fermentation process allows recovery of products with low boiling point from the off-gas via condensation. RESULTS: In this study we analyzed the production of acetone from syngas with the hypothetical production host derived from Moorella thermoacetica in a bubble column reactor at 60 °C with respect to thermodynamic and economic feasibility...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616073/the-transcription-factor-pdr-1-is-a-multi-functional-regulator-and-key-component-of-pectin-deconstruction-and-catabolism-in-neurospora-crassa
#5
Nils Thieme, Vincent W Wu, Axel Dietschmann, Asaf A Salamov, Mei Wang, Jenifer Johnson, Vasanth R Singan, Igor V Grigoriev, N Louise Glass, Chris R Somerville, J Philipp Benz
BACKGROUND: Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role for industrial purposes, such as for foodstuff processing. However, the regulation of pectinase gene expression is still poorly understood. For an optimal utilization of plant biomass for biorefinery and biofuel production, a detailed analysis of the underlying regulatory mechanisms is warranted...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616072/the-advanced-strategy-for-enhancing-biobutanol-production-and-high-efficient-product-recovery-with-reduced-wastewater-generation
#6
Chuang Xue, Xiaotong Zhang, Jufang Wang, Min Xiao, Lijie Chen, Fengwu Bai
BACKGROUND: Butanol as an important chemical and potential fuel could be produced via ABE fermentation from lignocellulosic biomass. The use of food-related feedstocks such as maize and sugar cane may not be a sustainable solution to world's energy needs. Recently, Jerusalem artichoke tubers containing inulin have been used as feedstock for butanol production, but this bioprocess is not commercially feasible due to the great value of inulin as functional food. Till now, there is a gap on the utilization of Jerusalem artichoke stalk (JAS) as feedstock for microbial butanol production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616071/combined-de-novo-and-ex-novo-lipid-fermentation-in-a-mix-medium-of-corncob-acid-hydrolysate-and-soybean-oil-by-trichosporon-dermatis
#7
Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Gao-Xiang Qi, Lian Xiong, Xiao-Qing Lin, Can Wang, Hai-Long Li, Xin-De Chen
BACKGROUND: Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and hydrophobic substrates can be utilized by oleaginous microorganisms with two different bio-pathways ("de novo" lipid fermentation and "ex novo" lipid fermentation). To date, most of the research on lipid fermentation has focused mainly on only one fermentation pathway and little work was carried out on both "de novo" and "ex novo" lipid fermentation simultaneously; thus, the advantages of both lipid fermentation cannot be fulfilled comprehensively...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28603553/sweet-sorghum-as-biofuel-feedstock-recent-advances-and-available-resources
#8
REVIEW
Supriya Mathur, A V Umakanth, V A Tonapi, Rita Sharma, Manoj K Sharma
Sweet sorghum is a promising target for biofuel production. It is a C4 crop with low input requirements and accumulates high levels of sugars in its stalks. However, large-scale planting on marginal lands would require improved varieties with optimized biofuel-related traits and tolerance to biotic and abiotic stresses. Considering this, many studies have been carried out to generate genetic and genomic resources for sweet sorghum. In this review, we discuss various attributes of sweet sorghum that make it an ideal candidate for biofuel feedstock, and provide an overview of genetic diversity, tools, and resources available for engineering and/or marker-assisting breeding of sweet sorghum...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28592994/effects-of-global-transcription-factor-ntca-on-photosynthetic-production-of-ethylene-in-recombinant-synechocystis-sp-pcc-6803
#9
Huilin Mo, Xiaoman Xie, Tao Zhu, Xuefeng Lu
BACKGROUND: Cyanobacteria are considered potential photosynthetic microbial cell factories for biofuel and biochemical production. Ethylene, one of the most important organic chemicals, has been successfully synthesized in cyanobacteria by introducing an exogenous ethylene-forming enzyme (Efe). However, it remains challenging to significantly improve the biosynthetic efficiency of cyanobacterial ethylene. Genetic modification of transcription factors is a powerful strategy for reprogramming cellular metabolism toward target products...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28592993/can-we-use-short-rotation-coppice-poplar-for-sugar-based-biorefinery-feedstock-bioconversion-of-2-year-old-poplar-grown-as-short-rotation-coppice
#10
Chang Dou, Wilian F Marcondes, Jessica E Djaja, Renata Bura, Rick Gustafson
BACKGROUND: Feedstock cost is a substantial barrier to the commercialization of lignocellulosic biorefineries. Poplar grown using a short rotation coppice (SRC) system has the potential to provide a low-cost feedstock and economically viable sugar yields for fuels and chemicals production. In the coppice management regime, poplars are harvested after 2 years' growth to develop the root system and establish the trees. The biomass from these 2-year-old trees is very heterogeneous, and includes components of leaf, bark, branch, and wood chip...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28588644/directed-evolution-of-a-%C3%AE-mannanase-from-rhizomucor-miehei-to-improve-catalytic-activity-in-acidic-and-thermophilic-conditions
#11
Yan-Xiao Li, Ping Yi, Qiao-Juan Yan, Zhen Qin, Xue-Qiang Liu, Zheng-Qiang Jiang
BACKGROUND: β-Mannanase randomly cleaves the β-1,4-linked mannan backbone of hemicellulose, which plays the most important role in the enzymatic degradation of mannan. Although the industrial applications of β-mannanase have tremendously expanded in recent years, the wild-type β-mannanases are still defective for some industries. The glycoside hydrolase (GH) family 5 β-mannanase (RmMan5A) from Rhizomucor miehei shows many outstanding properties, such as high specific activity and hydrolysis property...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28588643/molecular-cloning-expression-and-characterization-of-four-novel-thermo-alkaliphilic-enzymes-retrieved-from-a-metagenomic-library
#12
Mukil Maruthamuthu, Jan Dirk van Elsas
BACKGROUND: Enzyme discovery is a promising approach to aid in the deconstruction of recalcitrant plant biomass in an industrial process. Novel enzymes can be readily discovered by applying metagenomics on whole microbiomes. Our goal was to select, examine, and characterize eight novel glycoside hydrolases that were previously detected in metagenomic libraries, to serve biotechnological applications with high performance. RESULTS: Here, eight glycosyl hydrolase family candidate genes were selected from metagenomes of wheat straw-degrading microbial consortia using molecular cloning and subsequent gene expression studies in Escherichia coli...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28580015/removal-of-the-product-from-the-culture-medium-strongly-enhances-free-fatty-acid-production-by-genetically-engineered-synechococcus-elongatus
#13
Akihiro Kato, Nobuyuki Takatani, Kazutaka Ikeda, Shin-Ichi Maeda, Tatsuo Omata
BACKGROUND: Cyanobacterial mutants engineered for production of free fatty acids (FFAs) secrete the products to the medium and hence are thought to be useful for biofuel production. The dAS1T mutant constructed from Synechococcus elongatus PCC 7942 has indeed a large capacity of FFA production, which is comparable to that of triacylglycerol production in green algae, but the yield of secreted FFAs is low because the cells accumulate most of the FFAs intracellularly and eventually die of their toxicity...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28580014/methane-potentials-of-wastewater-generated-from-hydrothermal-liquefaction-of-rice-straw-focusing-on-the-wastewater-characteristics-and-microbial-community-compositions
#14
Huihui Chen, Cheng Zhang, Yue Rao, Yuhang Jing, Gang Luo, Shicheng Zhang
BACKGROUND: Hydrothermal liquefaction (HTL) has been well studied for the bio-oil production from biomass. However, a large amount of wastewater with high organic content is also produced during the HTL process. Therefore, the present study investigated the methane potentials of hydrothermal liquefaction wastewater (HTLWW) obtained from HTL of rice straw at different temperatures (170-320 °C) and residence times (0.5-4 h). The characteristics (e.g., total organic content, organic species, molecular size distribution, etc...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28580013/a-comparative-study-of-anaerobic-fixed-film-baffled-reactor-and-up-flow-anaerobic-fixed-film-fixed-bed-reactor-for-biological-removal-of-diethyl-phthalate-from-wastewater-a-performance-kinetic-biogas-and-metabolic-pathway-study
#15
Samira Yousefzadeh, Ehsan Ahmadi, Mitra Gholami, Hamid Reza Ghaffari, Ali Azari, Mohsen Ansari, Mohammad Miri, Kiomars Sharafi, Soheila Rezaei
BACKGROUND: Phthalic acid esters, including diethyl phthalate (DEP), which are considered as top-priority and hazardous pollutants, have received significant attention over the last decades. It is vital for industries to select the best treatment technology, especially when the DEP concentration in wastewater is high. Meanwhile, anaerobic biofilm-based reactors are considered as a promising option. Therefore, in the present study, for the biological removal of DEP from synthetic wastewater, two different anaerobic biofilm-based reactors, including anaerobic fixed film baffled reactor (AnFFBR) and up-flow anaerobic fixed film fixed bed reactor (UAnFFFBR), were compared from kinetic and performance standpoints...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28572841/de-novo-assembly-functional-annotation-and-analysis-of-the-giant-reed-arundo-donax-l-leaf-transcriptome-provide-tools-for-the-development-of-a-biofuel-feedstock
#16
Chiara Evangelistella, Alessio Valentini, Riccardo Ludovisi, Andrea Firrincieli, Francesco Fabbrini, Simone Scalabrin, Federica Cattonaro, Michele Morgante, Giuseppe Scarascia Mugnozza, Joost J B Keurentjes, Antoine Harfouche
BACKGROUND: Arundo donax has attracted renewed interest as a potential candidate energy crop for use in biomass-to-liquid fuel conversion processes and biorefineries. This is due to its high productivity, adaptability to marginal land conditions, and suitability for biofuel and biomaterial production. Despite its importance, the genomic resources currently available for supporting the improvement of this species are still limited. RESULTS: We used RNA sequencing (RNA-Seq) to de novo assemble and characterize the A...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28559928/novel-insight-into-the-relationship-between-organic-substrate-composition-and-volatile-fatty-acids-distribution-in-acidogenic-co-fermentation
#17
Huijun Ma, He Liu, Lihui Zhang, Meng Yang, Bo Fu, Hongbo Liu
BACKGROUND: Co-fermentation is an attractive technology for improving volatile fatty acids (VFAs) production by treatment of solid organic wastes. However, it remains unclear how the composition of different organic matters in solid waste influences the VFAs distribution, microbial community structure, and metabolic pathway during acidogenic co-fermentation. In this study, different organic wastes were added into waste activated sludge (WAS) as co-fermentation substrates to explore the impact of organic matter composition on VFAs pattern and the microbiological mechanism ...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28559927/modeling-the-impact-of-high-temperatures-on-microalgal-viability-and-photosynthetic-activity
#18
Quentin Béchet, Martin Laviale, Nicolas Arsapin, Hubert Bonnefond, Olivier Bernard
BACKGROUND: Culture collapse due to high temperatures can significantly impact the profitability of outdoor algal cultivation systems. The objective of this study was to model for the first time the impact of high temperatures on algal activity and viability. RESULTS: Viability measurements on Dunaliella salina cultures were based on cytometry with two fluorescent markers (erythrosine and fluorescein di-acetate), and photosynthetic activity was measured by Pulse Amplitude Modulation (PAM) fluorometry...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28559926/genome-wide-characterization-of-cellulases-from-the-hemi-biotrophic-plant-pathogen-bipolaris-sorokiniana-reveals-the-presence-of-a-highly-stable-gh7-endoglucanase
#19
Shritama Aich, Ravi K Singh, Pritha Kundu, Shree P Pandey, Supratim Datta
BACKGROUND: Bipolaris sorokiniana is a filamentous fungus that causes spot blotch disease in cereals like wheat and has severe economic consequences. However, information on the identities and role of the cell wall-degrading enzymes (CWDE) in B. sorokiniana is very limited. Several fungi produce CWDE like glycosyl hydrolases (GHs) that help in host cell invasion. To understand the role of these CWDE in B. sorokiniana, the first step is to identify and annotate all possible genes of the GH families like GH3, GH6, GH7, GH45 and AA9 and then characterize them biochemically...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28559925/integrated-analysis-of-454-and-illumina-transcriptomic-sequencing-characterizes-carbon-flux-and-energy-source-for-fatty-acid-synthesis-in-developing-lindera-glauca-fruits-for-woody-biodiesel
#20
Zixin Lin, Jiyong An, Jia Wang, Jun Niu, Chao Ma, Libing Wang, Guanshen Yuan, Lingling Shi, Lili Liu, Jinsong Zhang, Zhixiang Zhang, Ji Qi, Shanzhi Lin
BACKGROUND: Lindera glauca fruit with high quality and quantity of oil has emerged as a novel potential source of biodiesel in China, but the molecular regulatory mechanism of carbon flux and energy source for oil biosynthesis in developing fruits is still unknown. To better develop fruit oils of L. glauca as woody biodiesel, a combination of two different sequencing platforms (454 and Illumina) and qRT-PCR analysis was used to define a minimal reference transcriptome of developing L...
2017: Biotechnology for Biofuels
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