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Biotechnology for Biofuels

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https://www.readbyqxmd.com/read/28428823/combining-manipulation-of-transcription-factors-and-overexpression-of-the-target-genes-to-enhance-lignocellulolytic-enzyme-production-in-penicillium-oxalicum
#1
Liwei Gao, Zhonghai Li, Chengqiang Xia, Yinbo Qu, Meng Liu, Piao Yang, Lele Yu, Xin Song
BACKGROUND: Lignocellulolytic enzymes are the main enzymes to saccharify lignocellulose from renewable plant biomass in the bio-based economy. The production of these enzymes is transcriptionally regulated by multiple transcription factors. We previously engineered Penicillium oxalicum for improved cellulase production via manipulation of three genes in the cellulase expression regulatory network. However, the potential of combinational engineering of multiple regulators and their targets at protein abundance and activity levels has not been fully explored...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428822/in-muro-deacetylation-of-xylan-affects-lignin-properties-and-improves-saccharification-of-aspen-wood
#2
Prashant Mohan-Anupama Pawar, Marta Derba-Maceluch, Sun-Li Chong, Madhavi Latha Gandla, Shamrat Shafiul Bashar, Tobias Sparrman, Patrik Ahvenainen, Mattias Hedenström, Merve Özparpucu, Markus Rüggeberg, Ritva Serimaa, Martin Lawoko, Maija Tenkanen, Leif J Jönsson, Ewa J Mellerowicz
BACKGROUND: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428821/genome-wide-search-for-candidate-genes-for-yeast-robustness-improvement-against-formic-acid-reveals-novel-susceptibility-trk1-and-positive-regulators-and-resistance-haa1-regulon-determinants
#3
Sílvia F Henriques, Nuno P Mira, Isabel Sá-Correia
BACKGROUND: Formic acid is an inhibitory compound present in lignocellulosic hydrolysates. Understanding the complex molecular mechanisms underlying Saccharomyces cerevisiae tolerance to this weak acid at the system level is instrumental to guide synthetic pathway engineering for robustness improvement of industrial strains envisaging their use in lignocellulosic biorefineries. RESULTS: This study was performed to identify, at a genome-wide scale, genes whose expression confers protection or susceptibility to formic acid, based on the screening of a haploid deletion mutant collection to search for these phenotypes in the presence of 60, 70 and 80 mM of this acid, at pH 4...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428820/regional-water-footprints-of-potential-biofuel-production-in-china
#4
Xiaomin Xie, Tingting Zhang, Liming Wang, Zhen Huang
BACKGROUND: Development of biofuels is considered as one of the important ways to replace conventional fossil energy and mitigate climate change. However, rapid increase of biofuel production could cause other environmental concerns in China such as water stress. This study is intended to evaluate the life-cycle water footprints (WF) of biofuels derived from several potential non-edible feedstocks including cassava, sweet sorghum, and Jatropha curcas in China. Different water footprint types including blue water, green water, and grey water are considered in this study...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428819/lipid-production-and-molecular-dynamics-simulation-for-regulation-of-accd-gene-in-cyanobacteria-under-different-n-and-p-regimes
#5
Roshan Kumar, Koushik Biswas, Puneet Kumar Singh, Pankaj Kumar Singh, Sanniyasi Elumalai, Pratyoosh Shukla, Sunil Pabbi
BACKGROUND: Microalgae grown under different nutrient deficient conditions present a good source of natural lipids with applications for several types of biofuels. The expression of acetyl-CoA carboxylase gene can further provide an insight to the mechanisms leading to enhanced lipid production under such stresses. In this study, two nutrients viz. nitrogen and phosphorus were modulated to see its effect on lipid productivity in selected cyanobacteria and its correlation with Accase followed by molecular dynamics simulation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28416967/rescuing-ethanol-photosynthetic-production-of-cyanobacteria-in-non-sterilized-outdoor-cultivations-with-a-bicarbonate-based-ph-rising-strategy
#6
Zhi Zhu, Guodong Luan, Xiaoming Tan, Haocui Zhang, Xuefeng Lu
BACKGROUND: Ethanol photosynthetic production based on cyanobacteria cell factories utilizing CO2 and solar energy provides an attractive solution for sustainable production of green fuels. However, the scaling up processes of cyanobacteria cell factories were usually threatened or even devastated by biocontaminations, which restricted biomass or products accumulations of cyanobacteria cells. Thus it is of great significance to develop reliable biocontamination-controlling strategies for promoting ethanol photosynthetic production in large scales...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413447/enhancement-of-ethanol-production-from-green-liquor-ethanol-pretreated-sugarcane-bagasse-by-glucose-xylose-cofermentation-at-high-solid-loadings-with-mixed-saccharomyces-cerevisiae-strains
#7
Yanzhi You, Pengfei Li, Fuhou Lei, Yang Xing, Jianxin Jiang
BACKGROUND: Efficient cofermentation of glucose and xylose is necessary for economically feasible bioethanol production from lignocellulosic biomass. Here, we demonstrate pretreatment of sugarcane bagasse (SCB) with green liquor (GL) combined with ethanol (GL-Ethanol) by adding different GL amounts. The common Saccharomyces cerevisiae (CSC) and thermophilic S. cerevisiae (TSC) strains were used and different yeast cell mass ratios (CSC to TSC) were compared. The simultaneous saccharification and cofermentation (SSF/SSCF) process was performed by 5-20% (w/v) dry substrate (DS) solid loadings to determine optimal conditions for the co-consumption of glucose and xylose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413446/artificial-mirna-inhibition-of-phosphoenolpyruvate-carboxylase-increases-fatty-acid-production-in-a-green-microalga-chlamydomonas-reinhardtii
#8
Chaogang Wang, Xi Chen, Hui Li, Jiangxin Wang, Zhangli Hu
BACKGROUND: Nutrient limitation, such as nitrogen depletion, is the most widely used method for improving microalgae fatty acid production; however, these harsh conditions also inhibit algal growth significantly and even kill cells at all. To avoid these problems, we used artificial microRNA (amiRNA) technology as a useful tool to manipulate metabolic pathways to increase fatty acid contents effectively in the green microalga Chlamydomonas reinhardtii. We down-regulated the expression of phosphoenolpyruvate carboxylase (PEPC), which catalyzes the formation of oxaloacetate from phosphoenolpyruvate and regulates carbon flux...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413445/valorization-of-untreated-rice-bran-towards-bioflocculant-using-a-lignocellulose-degrading-strain-and-its-use-in-microalgal-biomass-harvest
#9
Cong Liu, Yan Hao, Jihong Jiang, Weijie Liu
BACKGROUND: Microalgae are currently considered as a promising feedstock for the production of biofuels and high-value products. However, the efficient harvest of microalgal biomasses from their culture broth is a major challenge. The harvesting of algal biomass by flocculation combined with gravity sedimentation is more convenient and cost-effective than traditional methods such as centrifugation and filtration. Compared to inorganic and chemically synthetic flocculants, bioflocculants are a suitable choice for microalgal harvest due to their biodegradable and nontoxic properties...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413444/functional-photosystem-i-maintains-proper-energy-balance-during-nitrogen-depletion-in-chlamydomonas-reinhardtii-promoting-triacylglycerol-accumulation
#10
Mahmoud Gargouri, Philip D Bates, Jeong-Jin Park, Helmut Kirchhoff, David R Gang
BACKGROUND: Nutrient deprivation causes significant stress to the unicellular microalga, Chlamydomonas reinhardtii, which responds by significantly altering its metabolic program. Following N deprivation, the accumulation of starch and triacylglycerols (TAGs) is significantly altered following massive reprogramming of cellular metabolism. One protein that was found to change dramatically and early to this stress was TAB2, a photosystem I (PSI) translation initiation factor, whose transcript and protein levels increased significantly after only 30 min of N deprivation...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28413443/molecular-and-biochemical-characterization-of-recombinant-cel12b-cel8c-and-peh28-overexpressed-in-escherichia-coli-and-their-potential-in-biofuel-production
#11
Eman Ibrahim, Kim D Jones, Keith E Taylor, Ebtesam N Hosseney, Patrick L Mills, Jean M Escudero
BACKGROUND: The high crystallinity of cellulosic biomass myofibrils as well as the complexity of their intermolecular structure is a significant impediment for biofuel production. Cloning of celB-, celC-encoded cellulases (cel12B and cel8C) and peh-encoded polygalacturonase (peh28) from Pectobacterium carotovorum subsp. carotovorum (Pcc) was carried out in our previous study using Escherichia coli as a host vector. The current study partially characterizes the enzymes' molecular structures as well as their catalytic performance on different substrates which can be used to improve their potential for lignocellulosic biomass conversion...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28405217/bioprocessing-of-tea-oil-fruit-hull-with-acetic-acid-organosolv-pretreatment-in-combination-with-alkaline-h2o2
#12
Song Tang, Rukuan Liu, Fubao Fuelbiol Sun, Chunying Dong, Rui Wang, Zhongyuan Gao, Zhanying Zhang, Zhihong Xiao, Changzhu Li, Hui Li
BACKGROUND: As a natural renewable biomass, the tea oil fruit hull (TOFH) mainly consists of lignocellulose, together with some bioactive substances. Our earlier work constructed a two-stage solvent-based process, including one aqueous ethanol organosolv extraction and an atmospheric glycerol organosolv (AGO) pretreatment, for bioprocessing of the TOFH into diverse bioproducts. However, the AGO pretreatment is not as selective as expected in removing the lignin from TOFH, resulting in the limited delignification and simultaneously high cellulose loss...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400859/precise-pretreatment-of-lignocellulose-relating-substrate-modification-with-subsequent-hydrolysis-and-fermentation-to-products-and-by-products
#13
Fan Lü, Lina Chai, Liming Shao, Pinjing He
BACKGROUND: Pretreatment is a crucial step for valorization of lignocellulosic biomass into valuable products such as H2, ethanol, acids, and methane. As pretreatment can change several decisive factors concurrently, it is difficult to predict its effectiveness. Furthermore, the effectiveness of pretreatments is usually assessed by enzymatic digestibility or merely according to the yield of the target fermentation products. The present study proposed the concept of "precise pretreatment," distinguished the major decisive factors of lignocellulosic materials by precise pretreatment, and evaluated the complete profile of all fermentation products and by-products...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28400858/multi-product-biorefineries-from-lignocelluloses-a-pathway-to-revitalisation-of-the-sugar-industry
#14
Somayeh Farzad, Mohsen Ali Mandegari, Miao Guo, Kathleen F Haigh, Nilay Shah, Johann F Görgens
BACKGROUND: Driven by a range of sustainability challenges, e.g. climate change, resource depletion and expanding populations, a circular bioeconomy is emerging and expected to evolve progressively in the coming decades. South Africa along with other BRICS countries (Brazil, Russia, India and China) represents the emerging bioeconomy and contributes significantly to global sugar market. In our research, South Africa is used as a case study to demonstrate the sustainable design for the future biorefineries annexed to existing sugar industry...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28367229/robust-high-productivity-phototrophic-carbon-capture-at-high-ph-and-alkalinity-using-natural-microbial-communities
#15
Christine E Sharp, Sydney Urschel, Xiaoli Dong, Allyson L Brady, Greg F Slater, Marc Strous
BACKGROUND: Bioenergy with carbon capture and storage (BECCS) has come to be seen as one of the most viable technologies to provide the negative carbon dioxide emissions needed to constrain global temperatures. In practice, algal biotechnology is the only form of BECCS that could be realized at scale without compromising food production. Current axenic algae cultivation systems lack robustness, are expensive and generally have marginal energy returns. RESULTS: Here it is shown that microbial communities sampled from alkaline soda lakes, grown as biofilms at high pH (up to 10) and high alkalinity (up to 0...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28367228/upgrading-syngas-fermentation-effluent-using-clostridium-kluyveri-in-a-continuous-fermentation
#16
Sylvia Gildemyn, Bastian Molitor, Joseph G Usack, Mytien Nguyen, Korneel Rabaey, Largus T Angenent
BACKGROUND: The product of current syngas fermentation systems is an ethanol/acetic acid mixture and the goal is to maximize ethanol recovery. However, ethanol currently has a relatively low market value and its separation from the fermentation broth is energy intensive. We can circumvent these disadvantages of ethanol production by converting the dilute ethanol/acetic acid mixture into products with longer carbon backbones, which are of higher value and are more easily extracted than ethanol...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28360941/co-production-of-hydrogen-and-ethanol-from-glucose-in-escherichia-coli-by-activation-of-pentose-phosphate-pathway-through-deletion-of-phosphoglucose-isomerase-pgi-and-overexpression-of-glucose-6-phosphate-dehydrogenase-zwf-and-6-phosphogluconate-dehydrogenase
#17
Balaji Sundara Sekar, Eunhee Seol, Sunghoon Park
BACKGROUND: Biologically, hydrogen (H2) can be produced through dark fermentation and photofermentation. Dark fermentation is fast in rate and simple in reactor design, but H2 production yield is unsatisfactorily low as <4 mol H2/mol glucose. To address this challenge, simultaneous production of H2 and ethanol has been suggested. Co-production of ethanol and H2 requires enhanced formation of NAD(P)H during catabolism of glucose, which can be accomplished by diversion of glycolytic flux from the Embden-Meyerhof-Parnas (EMP) pathway to the pentose-phosphate (PP) pathway in Escherichia coli...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28360940/comprehensive-characterization-of-non-cellulosic-recalcitrant-cell-wall-carbohydrates-in-unhydrolyzed-solids-from-afex-pretreated-corn-stover
#18
Christa Gunawan, Saisi Xue, Sivakumar Pattathil, Leonardo da Costa Sousa, Bruce E Dale, Venkatesh Balan
BACKGROUND: Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass). Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28360939/techno-economic-assessment-of-hybrid-extraction-and-distillation-processes-for-furfural-production-from-lignocellulosic-biomass
#19
Le Cao Nhien, Nguyen Van Duc Long, Sangyong Kim, Moonyong Lee
BACKGROUND: Lignocellulosic biomass is one of the most promising alternatives for replacing mineral resources to overcome global warming, which has become the most important environmental issue in recent years. Furfural was listed by the National Renewable Energy Laboratory as one of the top 30 potential chemicals arising from biomass. However, the current production of furfural is energy intensive and uses inefficient technology. Thus, a hybrid purification process that combines extraction and distillation to produce furfural from lignocellulosic biomass was considered and investigated in detail to improve the process efficiency...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28360938/citrobacter-amalonaticus-y19-for-constitutive-expression-of-carbon-monoxide-dependent-hydrogen-production-machinery
#20
Satish Kumar Ainala, Eunhee Seol, Jung Rae Kim, Sunghoon Park
BACKGROUND: Citrobacter amalonaticus Y19 is a good biocatalyst for production of hydrogen (H2) from oxidation of carbon monoxide (CO) via the so-called water-gas-shift reaction (WGSR). It has a high H2-production activity (23.83 mmol H2 g(-1) cell h(-1)) from CO, and can grow well to a high density on various sugars. However, its H2-production activity is expressed only when CO is present as an inducer and in the absence of glucose. RESULTS: In order to avoid dependency on CO and glucose, in the present study, the native CO-inducible promoters of WGSR operons (CO dehydrogenase, CODH, and CODH-dependent hydrogenase, CO-hyd) in Y19 were carefully analyzed and replaced with strong and constitutive promoters screened from Y19...
2017: Biotechnology for Biofuels
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