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Biotechnology for Biofuels

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https://www.readbyqxmd.com/read/28546828/improving-the-thermostability-of-a-fungal-gh11-xylanase-via-site-directed-mutagenesis-guided-by-sequence-and-structural-analysis
#1
Nanyu Han, Huabiao Miao, Junmei Ding, Junjun Li, Yuelin Mu, Junpei Zhou, Zunxi Huang
BACKGROUND: Xylanases have been widely employed in many industrial processes, and thermophilic xylanases are in great demand for meeting the high-temperature requirements of biotechnological treatments. In this work, we aim to improve the thermostability of XynCDBFV, a glycoside hydrolase (GH) family 11 xylanase from the ruminal fungus Neocallimastix patriciarum, by site-directed mutagenesis. We report favorable mutations at the C-terminus from B-factor comparison and multiple sequence alignment...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#2
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28533815/efficient-itaconic-acid-production-from-glycerol-with-ustilago-vetiveriae-tz1
#3
Thiemo Zambanini, Hamed Hosseinpour Tehrani, Elena Geiser, Dorothee Merker, Sarah Schleese, Judith Krabbe, Joerg M Buescher, Guido Meurer, Nick Wierckx, Lars M Blank
BACKGROUND: The family of Ustilaginaceae is known for their capability to naturally produce industrially valuable chemicals from different carbon sources. Recently, several Ustilaginaceae were reported to produce organic acids from glycerol, which is the main side stream in biodiesel production. RESULTS: In this study, we present Ustilago vetiveriae as new production organism for itaconate synthesis from glycerol. In a screening of 126 Ustilaginaceae, this organism reached one of the highest titers for itaconate combined with a high-glycerol uptake rate...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28529543/pilot-scale-steam-explosion-pretreatment-with-2-naphthol-to-overcome-high-softwood-recalcitrance
#4
Thomas Pielhop, Janick Amgarten, Michael H Studer, Philipp Rudolf von Rohr
BACKGROUND: Steam explosion pretreatment has been examined in many studies for enhancing the enzymatic digestibility of lignocellulosic biomass and is currently the most common pretreatment method in commercial biorefineries. It is however not effective for overcoming the extremely high recalcitrance of softwood to biochemical conversion. Recent fundamental research in small-scale liquid hot water pretreatment has shown, though, that the addition of a carbocation scavenger like 2-naphthol can prevent lignin repolymerization and thus enhance the enzymatic digestibility of softwood cellulose...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28523077/enhanced-biomethane-production-rate-and-yield-from-lignocellulosic-ensiled-forage-ley-by-in-situ-anaerobic-digestion-treatment-with-endogenous-cellulolytic-enzymes
#5
Jutta Speda, Mikaela A Johansson, Anna Odnell, Martin Karlsson
BACKGROUND: Enzymatic treatment of lignocellulosic material for increased biogas production has so far focused on pretreatment methods. However, often combinations of enzymes and different physicochemical treatments are necessary to achieve a desired effect. This need for additional energy and chemicals compromises the rationale of using enzymes for low energy treatment to promote biogas production. Therefore, simpler and less energy intensive in situ anaerobic digester treatment with enzymes is desirable...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28523076/metaproteomics-guided-selection-of-targeted-enzymes-for-bioprospecting-of-mixed-microbial-communities
#6
Jutta Speda, Bengt-Harald Jonsson, Uno Carlsson, Martin Karlsson
BACKGROUND: Hitherto, the main goal of metaproteomic analyses has been to characterize the functional role of particular microorganisms in the microbial ecology of various microbial communities. Recently, it has been suggested that metaproteomics could be used for bioprospecting microbial communities to query for the most active enzymes to improve the selection process of industrially relevant enzymes. In the present study, to reduce the complexity of metaproteomic samples for targeted bioprospecting of novel enzymes, a microbial community capable of producing cellulases was maintained on a chemically defined medium in an enzyme suppressed metabolic steady state...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28515786/evaluation-of-biomass-quality-in-short-rotation-bamboo-phyllostachys-pubescens-for-bioenergy-products
#7
Seung Gon Wi, Dae-Seok Lee, Quynh Anh Nguyen, Hyeun-Jong Bae
BACKGROUND: In order to improve the availability of biomass, the concept of growing high yield biomass with short rotations and intensive culture has been introduced. Bamboo has become a feedstock of potential interest for future energy production due to its high productivity and short rotation time. The growth age of biomass is an important factor affecting the efficiency of bioconversion and pretreatment for bioenergy production. In this regard, more information is required on the morphology and chemical composition of bamboo for short-rotation biomass production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28515785/recombinant-expression-of-thermostable-processive-mteg5-endoglucanase-and-its-synergism-with-mtlpmo-from-myceliophthora-thermophila-during-the-hydrolysis-of-lignocellulosic-substrates
#8
Anthi Karnaouri, Madhu Nair Muraleedharan, Maria Dimarogona, Evangelos Topakas, Ulrika Rova, Mats Sandgren, Paul Christakopoulos
BACKGROUND: Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28515784/improving-saccharomyces-cerevisiae-ethanol-production-and-tolerance-via-rna-polymerase-ii-subunit-rpb7
#9
Zilong Qiu, Rongrong Jiang
BACKGROUND: Classical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve Saccharomyces cerevisiae ethanol tolerance and productivity by reprogramming its transcription profile through rewiring its key transcription component RNA polymerase II (RNAP II), which plays a central role in synthesizing mRNAs. This is the first report on using directed evolution method to engineer RNAP II to alter S. cerevisiae strain phenotypes...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28507596/efficient-whole-cell-catalyzing-cellulose-saccharification-using-engineered-clostridium-thermocellum
#10
Jie Zhang, Shiyue Liu, Renmin Li, Wei Hong, Yan Xiao, Yingang Feng, Qiu Cui, Ya-Jun Liu
BACKGROUND: Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost catalyst for lignocellulose saccharification. The industrial use of C. thermocellum is restrained due largely to the inhibition of the hydrolysate cellobiose to its cellulosome. Although the supplementation of beta-glucosidase may solve the problem, the production of the enzymes greatly complicates the process and may also increase the cost of saccharification...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28503198/dynamics-of-two-methanogenic-microbiomes-incubated-in-polycyclic-aromatic-hydrocarbons-naphthenic-acids-and-oil-field-produced-water
#11
Bonahis J Oko, Yu Tao, David C Stuckey
BACKGROUND: Oil field produced water (OFPW) is widely produced in large volumes around the world. Transforming the organic matter in OFPW into bioenergy, such as biomethane, is one promising way to sustainability. However, OFPW is difficult to biologically degrade because it contains complex compounds such as naphthenic acids (NAs), or polycyclic aromatic hydrocarbons (PAHs). Although active microbial communities have been found in many oil reservoirs, little is known about how an exotic microbiome, e...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28503197/the-significance-of-proline-and-glutamate-on-butanol-chaotropic-stress-in-bacillus-subtilis-168
#12
Gumpanat Mahipant, Atchara Paemanee, Sittiruk Roytrakul, Junichi Kato, Alisa S Vangnai
BACKGROUND: Butanol is an intensively used industrial solvent and an attractive alternative biofuel, but the bioproduction suffers from its high toxicity. Among the native butanol producers and heterologous butanol-producing hosts, Bacillus subtilis 168 exhibited relatively higher butanol tolerance. Nevertheless, organic solvent tolerance mechanisms in Bacilli and Gram-positive bacteria have relatively less information. Thus, this study aimed to elucidate butanol stress responses that may involve in unique tolerance of B...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28503196/synthesis-and-analysis-of-separation-networks-for-the-recovery-of-intracellular-chemicals-generated-from-microbial-based-conversions
#13
Kirti M Yenkie, Wenzhao Wu, Christos T Maravelias
BACKGROUND: Bioseparations can contribute to more than 70% in the total production cost of a bio-based chemical, and if the desired chemical is localized intracellularly, there can be additional challenges associated with its recovery. Based on the properties of the desired chemical and other components in the stream, there can be multiple feasible options for product recovery. These options are composed of several alternative technologies, performing similar tasks. The suitability of a technology for a particular chemical depends on (1) its performance parameters, such as separation efficiency; (2) cost or amount of added separating agent; (3) properties of the bioreactor effluent (e...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28503195/strategies-for-improved-isopropanol-butanol-production-by-a-clostridium-strain-from-glucose-and-hemicellulose-through-consolidated-bioprocessing
#14
Fengxue Xin, Tianpeng Chen, Yujiang Jiang, Weiliang Dong, Wenming Zhang, Min Zhang, Hao Wu, Jiangfeng Ma, Min Jiang
BACKGROUND: High cost of traditional substrates and formation of by-products (such as acetone and ethanol) in acetone-butanol-ethanol (ABE) fermentation hindered the large-scale production of biobutanol. Here, we comprehensively characterized a newly isolated solventogenic and xylanolytic Clostridium species, which could produce butanol at a high ratio with elimination of ethanol and conversion of acetone to more value-added product, isopropanol. Ultimately, direct butanol production from hemicellulose was achieved with efficient expression of indigenous xylanase by the novel strain via consolidated bioprocessing...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491137/boosting-lpmo-driven-lignocellulose-degradation-by-polyphenol-oxidase-activated-lignin-building-blocks
#15
Matthias Frommhagen, Sumanth Kumar Mutte, Adrie H Westphal, Martijn J Koetsier, Sandra W A Hinz, Jaap Visser, Jean-Paul Vincken, Dolf Weijers, Willem J H van Berkel, Harry Gruppen, Mirjam A Kabel
BACKGROUND: Many fungi boost the deconstruction of lignocellulosic plant biomass via oxidation using lytic polysaccharide monooxygenases (LPMOs). The application of LPMOs is expected to contribute to ecologically friendly conversion of biomass into fuels and chemicals. Moreover, applications of LPMO-modified cellulose-based products may be envisaged within the food or material industry. RESULTS: Here, we show an up to 75-fold improvement in LPMO-driven cellulose degradation using polyphenol oxidase-activated lignin building blocks...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#16
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484517/h2-production-pathways-in-nutrient-replete-mixotrophic-chlamydomonas-cultures-under-low-light-response-to-the-commentary-article-on-the-pathways-feeding-the-h2-production-process-in-nutrient-replete-hypoxic-conditions-by-alberto-scoma-and-szilvia-z-t%C3%A3-th
#17
EDITORIAL
David González-Ballester, Jose Luis Jurado-Oller, Aurora Galván, Emilio Fernández, Alexandra Dubini
BACKGROUND: A recent Commentary article entitled "On the pathways feeding the H2 production process in nutrient-replete, hypoxic conditions" by Dr. Scoma and Dr. Tóth, Biotechnology for Biofuels (2017), opened a very interesting debate about the H2 production photosynthetic-linked pathways occurring in Chlamydomonas cultures grown in acetate-containing media and incubated under hypoxia/anoxia conditions. This Commentary article mainly focused on the results of our previous article "Low oxygen levels contribute to improve photohydrogen production in mixotrophic non-stressed Chlamydomonas cultures," by Jurado-Oller et al...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484516/on-the-pathways-feeding-the-h2-production-process-in-nutrient-replete-hypoxic-conditions-commentary-on-the-article-low-oxygen-levels-contribute-to-improve-photohydrogen-production-in-mixotrophic-non-stressed-chlamydomonas-cultures-by-jurado-oller-et-al-biotechnology
#18
EDITORIAL
Alberto Scoma, Szilvia Z Tóth
BACKGROUND: Under low O2 concentration (hypoxia) and low light, Chlamydomonas cells can produce H2 gas in nutrient-replete conditions. This process is hindered by the presence of O2, which inactivates the [FeFe]-hydrogenase enzyme responsible for H2 gas production shifting algal cultures back to normal growth. The main pathways accounting for H2 production in hypoxia are not entirely understood, as much as culture conditions setting the optimal redox state in the chloroplast supporting long-lasting H2 production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484515/cultivation-of-algal-biofilm-using-different-lignocellulosic-materials-as-carriers
#19
Qi Zhang, Cuixia Liu, Yubiao Li, Zhigang Yu, Zhihua Chen, Ting Ye, Xun Wang, Zhiquan Hu, Shiming Liu, Bo Xiao, Shiping Jin
BACKGROUND: Algal biofilm technology is recently supposed to be a promising method to produce algal biomass as the feedstock for the production of biofuels. However, the carrier materials currently used to form algal biofilm are either difficult to be obtained at a low price or undurable. Commercialization of the biofilm technology for algal biomass production extremely requires new and inexpensive materials as biofilm carriers with high biomass production performances. RESULTS: Four types of lignocellulosic materials were investigated to evaluate their performance of acting as carriers for algal cells attachment and the relevant effects on the algal biomass production in this study...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28484514/increased-lignocellulosic-inhibitor-tolerance-of-saccharomyces-cerevisiae-cell-populations-in-early-stationary-phase
#20
Venkatachalam Narayanan, Jenny Schelin, Marie Gorwa-Grauslund, Ed Wj van Niel, Magnus Carlquist
BACKGROUND: Production of second-generation bioethanol and other bulk chemicals by yeast fermentation requires cells that tolerate inhibitory lignocellulosic compounds at low pH. Saccharomyces cerevisiae displays high plasticity with regard to inhibitor tolerance, and adaptation of cell populations to process conditions is essential for reaching efficient and robust fermentations. RESULTS: In this study, we assessed responses of isogenic yeast cell populations in different physiological states to combinations of acetic acid, vanillin and furfural at low pH...
2017: Biotechnology for Biofuels
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