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Microbial Biotechnology

Marco Ventura, Christian Milani, Gabriele A Lugli, Douwe van Sinderen
Development of the human gut throughout the entire life.
November 8, 2018: Microbial Biotechnology
Beatrice Ramm, Petra Schwille
In vitro reconstitution of bacterial cytoskeletal elements, primarily supposed to reveal detailed mechanistic insights, has been an invaluable source of unexpected new protein functionalities. This may be particularly beneficial in the context of a potential construction of artificial cells from the bottom-up.
November 8, 2018: Microbial Biotechnology
Oliver Drzyzga, Auxiliadora Prieto
No abstract text is available yet for this article.
November 8, 2018: Microbial Biotechnology
Pengsong Li, Xiaofen Fu, Lei Zhang, Shizhong Li
CRISPR/Cas-based (clustered regularly interspaced short palindromic repeats/CRISPR-associated) screening has been proved to be an efficient method to study functional genomics from yeast to human. In this study, we report the development of a focused CRISPR/Cas-based gene activation library in Saccharomyces cerevisiae and its application in gene identification based on functional screening towards improved thermotolerance. The gene activation library was subjected to screening at 42°C, and the same library cultured at 30°C was set as a control group...
November 5, 2018: Microbial Biotechnology
Martin Alexander Fischer, Simon Güllert, Sarah Refai, Sven Künzel, Uwe Deppenmeier, Wolfgang R Streit, Ruth Anne Schmitz
Ammonia caused disturbance of biogas production is one of the most frequent incidents in regular operation of biogas reactors. This study provides a detailed insight into the microbial community of a mesophilic, full-scale biogas reactor (477 kWh h-1 ) fed with maize silage, dried poultry manure and cow manure undergoing initial process disturbance by increased ammonia concentration. Over a time period of 587 days, the microbial community of the reactor was regularly monitored on a monthly basis by high-throughput amplicon sequencing of the archaeal and bacterial 16S rRNA genes...
October 31, 2018: Microbial Biotechnology
Víctor de Lorenzo, Philippe Marliére
No abstract text is available yet for this article.
October 25, 2018: Microbial Biotechnology
Chrispian W Theron, Michel Labuschagné, Jacobus Albertyn, Martha S Smit
Cytochrome P450 monooxygenases (P450) are enzymes with high potential as biocatalysts for industrial applications. Their large-scale applications are, however, limited by instability and requirement for coproteins and/or expensive cofactors. These problems are largely overcome when whole cells are used as biocatalysts. We previously screened various yeast species heterologously expressing self-sufficient P450s for their potential as whole-cell biocatalysts. Most P450s are, however, not self-sufficient and consist of two or three protein component systems...
October 19, 2018: Microbial Biotechnology
Wanderson M Silva, Cassiana S Sousa, Leticia C Oliveira, Siomar C Soares, Gustavo F M H Souza, Guilherme C Tavares, Cristiana P Resende, Edson L Folador, Felipe L Pereira, Henrique Figueiredo, Vasco Azevedo
Lactococcus lactis is a bacteria with high biotechnological potential, where is frequently used in the amino acid production and production of fermented dairy products, as well as drug delivery systems and mucosal vaccine vector. The knowledge of a functional core proteome is important extremely for both fundamental understanding of cell functions and for synthetic biology applications. In this study, we characterized the L. lacits proteome from proteomic analysis of four biotechnological strains L. lactis: L...
October 19, 2018: Microbial Biotechnology
Prithiviraj Chellamuthu, Frances Tran, Kalinga Pavan T Silva, Marko S Chavez, Mohamed Y El-Naggar, James Q Boedicker
Microbes naturally build nanoscale structures, including structures assembled from inorganic materials. Here, we combine the natural capabilities of microbes with engineered genetic control circuits to demonstrate the ability to control biological synthesis of chalcogenide nanomaterials in a heterologous host. We transferred reductase genes from both Shewanella sp. ANA-3 and Salmonella enterica serovar Typhimurium into a heterologous host (Escherichia coli) and examined the mechanisms that regulate the properties of biogenic nanomaterials...
October 17, 2018: Microbial Biotechnology
Linda S Thomashow, Melissa K LeTourneau, Youn-Sig Kwak, David M Weller
Future efforts to increase agricultural productivity will focus on crops as functional units comprised of plants and their associated microflora in the context of the various environments in which they are grown. It is suggested that future efforts to increase agricultural productivity will focus on crops as functional units comprised of plants and their associated beneficial microorganisms in the context in which they are grown. Scientists, industry, and farmers must work closely together to develop, adapt, and apply new technologies to a wide range of cropping systems...
October 16, 2018: Microbial Biotechnology
Cristina Vilanova, Manuel Porcar
No abstract text is available yet for this article.
October 16, 2018: Microbial Biotechnology
Agnieszka Sekowska, Hiroki Ashida, Antoine Danchin
Methionine is essential for life. Its chemistry makes it fragile in the presence of oxygen. Aerobic living organisms have selected a salvage pathway (the MSP) that uses dioxygen to regenerate methionine, associated to a ratchet-like step that prevents methionine back degradation. Here, we describe the variation on this theme, developed across the tree of life. Oxygen appeared long after life had developed on Earth. The canonical MSP evolved from ancestors that used both predecessors of ribulose bisphosphate carboxylase oxygenase (RuBisCO) and methanethiol in intermediate steps...
October 10, 2018: Microbial Biotechnology
Nina Urbelienė, Simonas Kutanovas, Rita Meškienė, Renata Gasparavičiūtė, Daiva Tauraitė, Martyna Koplūnaitė, Rolandas Meškys
A high-throughput method (≥ 106 of clones can be analysed on a single agar plate) for the selection of ester-hydrolysing enzymes was developed based on the uridine auxotrophy of Escherichia coli strain DH10B ΔpyrFEC and the acylated derivatives 2',3',5'-O-tri-acetyluridine and 2',3',5'-O-tri-hexanoyluridine as the sole source of uridine. The proposed approach permits the selection of hydrolases belonging to different families and active towards different substrates. Moreover, the ester group of the substrate used for the selection, at least partly, determined the specificity of the selected enzymes...
October 9, 2018: Microbial Biotechnology
Liang Cheng, Yang Yang, Jian Chu
This paper presents a novel approach of using in-situ microbially induced Ca2+ -alginate polymeric sealant for seepage control in porous materials. This process comprises two steps: (i) generation of insoluble calcium carbonate inside the pores of porous materials (such as sand) through a microbially induced carbonate precipitation (MICP) process in-situ and (ii) injection of sodium alginate for in-situ gelation via reaction between alginate and Ca2+ ions. The experimental results showed that the hydraulic conductivity/permeability of sand decreased with the increase in alginate concentration...
October 6, 2018: Microbial Biotechnology
Mario González, Nélida Brito, Eduardo Hernández-Bolaños, Celedonio González
Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well-established and widely used technique. It typically requires the construction of an expression vector in Escherichia coli containing the foreign gene and its subsequent transformation into yeast. Although simple, this procedure has important limitations for the expression of large numbers of proteins, that is, for the generation of protein libraries. We describe here the development of a novel system for the easy and fast expression of heterologous proteins both in S...
October 5, 2018: Microbial Biotechnology
Gloria Andrea Silva-Castro, Anesu Conrad Moyo, Londiwe Khumalo, Leonardo Joaquim van Zyl, Leslie F Petrik, Marla Trindade
The disposal of reject brine, a highly concentrated waste by-product generated by various industrial processes, represents a major economic and environmental challenge. The common practice in dealing with the large amounts of brine generated is to dispose of it in a pond and allow it to evaporate. The rate of evaporation is therefore a key factor in the effectiveness of the management of these ponds. The addition of various dyes has previously been used as a method to increase the evaporation rate. In this study, a biological approach, using pigmented halophilic bacteria (as opposed to chemical dyes), was assessed...
October 2, 2018: Microbial Biotechnology
Luísa Czamanski Nora, Cauã Antunes Westmann, Leonardo Martins-Santana, Luana de Fátima Alves, Lummy Maria Oliveira Monteiro, María-Eugenia Guazzaroni, Rafael Silva-Rocha
When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously expanding and undergoing adaptations. Here, we provide a detailed view following the evolution of vectors built throughout the years destined to study microorganisms and their peculiarities, including those whose genomes can only be revealed through metagenomics...
September 26, 2018: Microbial Biotechnology
Dick de Ridder
No abstract text is available yet for this article.
September 24, 2018: Microbial Biotechnology
Concha García, María Auxiliadora Prieto
Shoe patterns and a sole made of BC, baby shoe made of BC and, on the right BC material dyed in red. (prototype made by
August 22, 2018: Microbial Biotechnology
Lawrence P Wackett
No abstract text is available yet for this article.
November 2018: Microbial Biotechnology
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