journal
https://read.qxmd.com/read/38402618/region-specific-cellular-and-molecular-basis-of-liver-regeneration-after-acute-pericentral-injury
#21
JOURNAL ARTICLE
Shuyong Wang, Xuan Wang, Yiran Shan, Zuolong Tan, Yuxin Su, Yannan Cao, Shuang Wang, Jiahong Dong, Jin Gu, Yunfang Wang
Liver injuries often occur in a zonated manner. However, detailed regenerative responses to such zonal injuries at cellular and molecular levels remain largely elusive. By using a fate-mapping strain, Cyp2e1-DreER, to elucidate liver regeneration after acute pericentral injury, we found that pericentral regeneration is primarily compensated by the expansion of remaining pericentral hepatocytes, and secondarily by expansion of periportal hepatocytes. Employing single-cell RNA sequencing, spatial transcriptomics, immunostaining, and in vivo functional assays, we demonstrated that the upregulated expression of the mTOR/4E-BP1 axis and lactate dehydrogenase A in hepatocytes contributes to pericentral regeneration, while activation of transforming growth factor β (TGF-β1) signaling in the damaged area mediates fibrotic responses and inhibits hepatocyte proliferation...
March 7, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306996/preclinical-and-dose-ranging-assessment-of-hesc-derived-dopaminergic-progenitors-for-a-clinical-trial-on-parkinson-s-disease
#22
Sanghyun Park, Chan Wook Park, Jang Hyeon Eom, Mi-Young Jo, Hye-Jin Hur, Sung Kyoung Choi, Jae Souk Lee, Seung Taek Nam, Ki-Sang Jo, Young Woo Oh, Jungil Lee, Sieun Kim, Do-Hun Kim, Chul-Yong Park, Su Jin Kim, Ho-Young Lee, Myung Soo Cho, Dae-Sung Kim, Dong-Wook Kim
No abstract text is available yet for this article.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306995/srcap-mutations-drive-clonal-hematopoiesis-through-epigenetic-and-dna-repair-dysregulation
#23
Chun-Wei Chen, Linda Zhang, Ravi Dutta, Abhishek Niroula, Peter G Miller, Christopher J Gibson, Alexander G Bick, Jaime M Reyes, Yi-Tang Lee, Ayala Tovy, Tianpeng Gu, Sarah Waldvogel, Yi-Hung Chen, Bryan J Venters, Pierre-Olivier Estève, Sriharsa Pradhan, Michael-Christopher Keogh, Pradeep Natarajan, Koichi Takahashi, Adam S Sperling, Margaret A Goodell
No abstract text is available yet for this article.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306994/3d-bioprinting-of-human-neural-tissues-with-functional-connectivity
#24
JOURNAL ARTICLE
Yuanwei Yan, Xueyan Li, Yu Gao, Sakthikumar Mathivanan, Linghai Kong, Yunlong Tao, Yi Dong, Xiang Li, Anita Bhattacharyya, Xinyu Zhao, Su-Chun Zhang
Probing how human neural networks operate is hindered by the lack of reliable human neural tissues amenable to the dynamic functional assessment of neural circuits. We developed a 3D bioprinting platform to assemble tissues with defined human neural cell types in a desired dimension using a commercial bioprinter. The printed neuronal progenitors differentiate into neurons and form functional neural circuits within and between tissue layers with specificity within weeks, evidenced by the cortical-to-striatal projection, spontaneous synaptic currents, and synaptic response to neuronal excitation...
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306993/metabolic-regulation-of-the-hallmarks-of-stem-cell-biology
#25
REVIEW
Benjamin T Jackson, Lydia W S Finley
Stem cells perform many different functions, each of which requires specific metabolic adaptations. Over the past decades, studies of pluripotent and tissue stem cells have uncovered a range of metabolic preferences and strategies that correlate with or exert control over specific cell states. This review aims to describe the common themes that emerge from the study of stem cell metabolism: (1) metabolic pathways supporting stem cell proliferation, (2) metabolic pathways maintaining stem cell quiescence, (3) metabolic control of cellular stress responses and cell death, (4) metabolic regulation of stem cell identity, and (5) metabolic requirements of the stem cell niche...
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306992/sham-surgery-for-the-trialing-of-cell-based-therapies-to-the-cns-may-not-be-necessary
#26
RANDOMIZED CONTROLLED TRIAL
Viviane Tabar, Roger A Barker
Sham surgery is often required for cell therapies adopting a randomized placebo-controlled double-blinded trial design. Using the case of dopamine neuron therapy for Parkinson's disease, we argue that alternative trial designs should be considered instead, for several reasons relating to ethics, patient burden, ease of unblinding, and cost.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306991/extra-embryonic-dialogues-keys-to-improved-stem-cell-based-embryo-models
#27
JOURNAL ARTICLE
Arun Pandian Chandrasekaran, Mo Li
Despite advances in stem cell cultures, modeling early human development with stem cells in a dish remains challenging. Research by Hislop et al.,1 Okubo et al.,2 and Wei et al.3 paves the way for improved in vitro embryo models and culture conditions, offering valuable insights for regenerative medicine.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306990/unraveling-the-brain-s-response-to-covid-19-how-sars-cov-2-afflicts-dopaminergic-neurons
#28
JOURNAL ARTICLE
Ilse Eidhof, Daniel Twohig, Anna Falk
COVID-19 patients often display dysfunctions of the nervous system, indicating an effect of SARS-CoV-2 on neural cells. Yang et al. now show that human stem-cell-derived dopaminergic neurons are susceptible to SARS-CoV-2, triggering inflammation and senescence. The study further identifies three FDA-approved drugs capable of reversing these cellular phenotypes.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306989/bioprinting-functional-neural-networks
#29
JOURNAL ARTICLE
Stephanie M Willerth
3D printing human tissue models derived from stem cells provides an increasingly popular tissue engineering strategy for probing biological questions. Here Yan et al.1 demonstrate how this technology can be used to model mature human neural tissues with functional neural networks in healthy and disease states.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38306988/what-is-the-scope-of-cell-stem-cell-now-and-in-the-future
#30
EDITORIAL
Sheila Chari, Catherine Landis, Quan Wang, Christine Weber
No abstract text is available yet for this article.
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38232727/exercise-induced-musclin-determines-the-fate-of-fibro-adipogenic-progenitors-to-control-muscle-homeostasis
#31
JOURNAL ARTICLE
Xia Kang, Jin Qian, You-Xing Shi, Xu-Ting Bian, Li-Dan Zhang, Gao-Ming Li, Li-Ting Wang, Jing Zhao, Zhen-Yu Dong, Meng-Meng Yang, Yu-Jia-Nan Chen, Kang-Lai Tang, Hong-Ming Miao
The effects of exercise on fibro-adipogenic progenitors (FAPs) are unclear, and the direct molecular link is still unknown. In this study, we reveal that exercise reduces the frequency of FAPs and attenuates collagen deposition and adipose formation in injured or disused muscles through Musclin. Mechanistically, Musclin inhibits FAP proliferation and promotes apoptosis in FAPs by upregulating FILIP1L. Chromatin immunoprecipitation (ChIP)-qPCR confirms that FoxO3a is the transcription factor of FILIP1L. In addition, the Musclin/FILIP1L pathway facilitates the phagocytosis of apoptotic FAPs by macrophages through downregulating the expression of CD47...
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38215738/human-conjunctiva-organoids-to-study-ocular-surface-homeostasis-and-disease
#32
JOURNAL ARTICLE
Marie Bannier-Hélaouët, Jeroen Korving, Ziliang Ma, Harry Begthel, Amir Giladi, Mart M Lamers, Willine J van de Wetering, Nobuyo Yawata, Makoto Yawata, Vanessa L S LaPointe, Mor M Dickman, Rachel Kalmann, Saskia M Imhoff, Johan H van Es, Carmen López-Iglesias, Peter J Peters, Bart L Haagmans, Wei Wu, Hans Clevers
The conjunctival epithelium covering the eye contains two main cell types: mucus-producing goblet cells and water-secreting keratinocytes, which present mucins on their apical surface. Here, we describe long-term expanding organoids and air-liquid interface representing mouse and human conjunctiva. A single-cell RNA expression atlas of primary and cultured human conjunctiva reveals that keratinocytes express multiple antimicrobial peptides and identifies conjunctival tuft cells. IL-4/-13 exposure increases goblet and tuft cell differentiation and drastically modifies the conjunctiva secretome...
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38183977/a-time-and-single-cell-resolved-model-of-murine-bone-marrow-hematopoiesis
#33
JOURNAL ARTICLE
Iwo Kucinski, Joana Campos, Melania Barile, Francesco Severi, Natacha Bohin, Pedro N Moreira, Lewis Allen, Hannah Lawson, Myriam L R Haltalli, Sarah J Kinston, Dónal O'Carroll, Kamil R Kranc, Berthold Göttgens
The paradigmatic hematopoietic tree model is increasingly recognized to be limited, as it is based on heterogeneous populations largely defined by non-homeostatic assays testing cell fate potentials. Here, we combine persistent labeling with time-series single-cell RNA sequencing to build a real-time, quantitative model of in vivo tissue dynamics for murine bone marrow hematopoiesis. We couple cascading single-cell expression patterns with dynamic changes in differentiation and growth speeds. The resulting explicit linkage between molecular states and cellular behavior reveals widely varying self-renewal and differentiation properties across distinct lineages...
February 1, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181752/hallmarks-of-stemness-in-mammalian-tissues
#34
REVIEW
Joep Beumer, Hans Clevers
All adult tissues experience wear and tear. Most tissues can compensate for cell loss through the activity of resident stem cells. Although the cellular maintenance strategies vary greatly between different adult (read: postnatal) tissues, the function of stem cells is best defined by their capacity to replace lost tissue through division. We discuss a set of six complementary hallmarks that are key enabling features of this basic function. These include longevity and self-renewal, multipotency, transplantability, plasticity, dependence on niche signals, and maintenance of genome integrity...
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181751/kidney-organoid-models-reveal-cilium-autophagy-metabolic-axis-as-a-therapeutic-target-for-pkd-both-in%C3%A2-vitro-and-in%C3%A2-vivo
#35
JOURNAL ARTICLE
Meng Liu, Chao Zhang, Ximing Gong, Tian Zhang, Michelle Mulan Lian, Elaine Guo Yan Chew, Angelysia Cardilla, Keiichiro Suzuki, Huamin Wang, Yuan Yuan, Yan Li, Mihir Yogesh Naik, Yixuan Wang, Bingrui Zhou, Wei Ze Soon, Emi Aizawa, Pin Li, Jian Hui Low, Moses Tandiono, Enrique Montagud, Daniel Moya-Rull, Concepcion Rodriguez Esteban, Yosu Luque, Mingliang Fang, Chiea Chuen Khor, Nuria Montserrat, Josep M Campistol, Juan Carlos Izpisua Belmonte, Jia Nee Foo, Yun Xia
Human pluripotent stem cell-derived kidney organoids offer unprecedented opportunities for studying polycystic kidney disease (PKD), which still has no effective cure. Here, we developed both in vitro and in vivo organoid models of PKD that manifested tubular injury and aberrant upregulation of renin-angiotensin aldosterone system. Single-cell analysis revealed that a myriad of metabolic changes occurred during cystogenesis, including defective autophagy. Experimental activation of autophagy via ATG5 overexpression or primary cilia ablation significantly inhibited cystogenesis in PKD kidney organoids...
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181750/scaling-up-gmp-grade-dopaminergic-cells-for-parkinson-s-disease
#36
JOURNAL ARTICLE
Thomas Foltynie
Stem cell therapy for Parkinson's disease requires demonstration of safety and efficacy of dopaminergic cells derived from a cell line, consideration of dose, and whether this is deliverable at scale. Park et al. demonstrate these requirements for a new hESC line and that their manufacturing methods allow for its scalability.
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181749/human-cerebellar-organoids-with-functional-purkinje-cells
#37
JOURNAL ARTICLE
Alexander Atamian, Marcella Birtele, Negar Hosseini, Tuan Nguyen, Anoothi Seth, Ashley Del Dosso, Sandeep Paul, Neil Tedeschi, Ryan Taylor, Marcelo P Coba, Ranmal Samarasinghe, Carlos Lois, Giorgia Quadrato
Research on human cerebellar development and disease has been hampered by the need for a human cell-based system that recapitulates the human cerebellum's cellular diversity and functional features. Here, we report a human organoid model (human cerebellar organoids [hCerOs]) capable of developing the complex cellular diversity of the fetal cerebellum, including a human-specific rhombic lip progenitor population that have never been generated in vitro prior to this study. 2-month-old hCerOs form distinct cytoarchitectural features, including laminar organized layering, and create functional connections between inhibitory and excitatory neurons that display coordinated network activity...
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181748/improving-cellular-diversity-in-human-cerebellar-organoids
#38
JOURNAL ARTICLE
Marilyn Steyert, Tomasz Nowakowski
The developing human cerebellum has a greater diversity of progenitor types than that of the mouse, necessitating a human-based model for studying cerebellar development and disease. Atamian et al.1 developed a 3D organoid model of cerebellar development, which recapitulates many cell types found in the developing human cerebellum, including Purkinje-neuron-like cells.
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181747/mechanosensitive-hormone-signaling-promotes-mammary-progenitor-expansion-and-breast-cancer-risk
#39
JOURNAL ARTICLE
Jason J Northey, Mary-Kate Hayward, Yoshihiro Yui, Connor Stashko, FuiBoon Kai, Janna K Mouw, Dhruv Thakar, Jonathon N Lakins, Alastair J Ironside, Susan Samson, Rita A Mukhtar, E Shelley Hwang, Valerie M Weaver
Tissue stem-progenitor cell frequency has been implicated in tumor risk and progression, but tissue-specific factors linking these associations remain ill-defined. We observed that stiff breast tissue from women with high mammographic density, who exhibit increased lifetime risk for breast cancer, associates with abundant stem-progenitor epithelial cells. Using genetically engineered mouse models of elevated integrin mechanosignaling and collagen density, syngeneic manipulations, and spheroid models, we determined that a stiff matrix and high mechanosignaling increase mammary epithelial stem-progenitor cell frequency and enhance tumor initiation in vivo...
January 4, 2024: Cell Stem Cell
https://read.qxmd.com/read/38181746/the-kras-tour-studying-metabolic-reprogramming-in-isogenic-pancreatic-cancer-organoids
#40
JOURNAL ARTICLE
Shree Bose, Xiling Shen
Using an isogenic organoid platform to model pancreatic cancer, Duan et al. establish an important link between mutant KRAS and cholesterol metabolism and identify perhexiline maleate as a possible therapeutic to target this relationship.
January 4, 2024: Cell Stem Cell
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