Yuanyuan Zhang, Tao Na, Kehua Zhang, Yanping Yang, Huanye Xu, Lina Wei, Liming Xu, Xiaojun Yan, Wei Liu, Guangyang Liu, Bin Wang, Shufang Meng, Yanan Du
Efficient and large-scale expansion of mesenchymal stem/stromal cells (MSCs) has always been a formidable challenge to researchers in cell-based therapies and regenerative medicine. To reconcile major drawbacks of 2D planar culturing system, we innovatively developed an automated closed industrial scale cell production (ACISCP) platform based on GMP-grade microcarrier for culture of umbilical cord-mesenchymal stem/stromal cells (UCMSCs), in accordance with the criteria of stem cell bank. ACISCP system is a fully closed system, which employs different models of vivaSPIN bioreactors (CytoNiche Biotech, China) for scale-up cell culture and vivaPREP (CytoNiche Biotech, China) for automated cell harvesting and cell dosage preparation...
October 2022: Journal of Tissue Engineering and Regenerative Medicine