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Journal of Tissue Engineering and Regenerative Medicine

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https://www.readbyqxmd.com/read/28548695/development-of-a-novel-bioabsorbable-implant-that-is-substituted-by-adipose-tissue-in-vivo
#1
Shuichi Ogino, Naoki Morimoto, Michiharu Sakamoto, Chizuru Jinno, Katsuhiro Yoshikawa, Tatsuki Enoshiri, Yuki Sakamoto, Tsuguyoshi Taira, Shigehiko Suzuki
Recently, adipose tissue has been regenerated by combining scaffolds, growth factors, and/or adipose tissue-derived stromal cells (ASCs). However, the safety of growth factors and ASCs has not been confirmed in cancer patients. We reported the regeneration of adipose tissue in the internal space of a polypropylene mesh containing a collagen sponge (CS), without using any growth factors or cells. We herein explored the formation of adipose tissue, using the bioabsorbable implant containing CS, in rats. We prepared the implants without and with CS, using threads of either poly l-lactide-co-ε-caprolactone [P(LA/CL)] or poly-l-lactic acid (PLLA), and measured their strengths...
May 26, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28548246/comparison-of-three-different-types-of-scaffolds-preseeded-with-human-bone-marrow-mononuclear-cells-on-the-bone-healing-in-a-femoral-critical-size-defect-model-of-the-athymic-rat
#2
Maren Janko, Julian Sahm, Alexander Schaible, Jan C Brune, Marlene Bellen, Katrin Schröder, Caroline Seebach, Ingo Marzi, Dirk Henrich
Large bone defects often pose major difficulties in orthopedic surgery. The application of long term cultured stem cells combined with a scaffold lead to a significant improvement of bone healing in recent experiments but is strongly restricted by EU-law. Bone marrow mononuclear cells (BMC), however, can be isolated and transplanted within few hours and have been proven effective in experimental models of bone healing. The effectivity of the BMC supported therapy might be influenced by the type of scaffold...
May 26, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28544701/intradiscal-application-of-a-pcla-peg-pcla-hydrogel-loaded-with-celecoxib-for-the-treatment-of-back-pain-in-canines-what-s-in-it-for-humans
#3
Anna R Tellegen, Nicole Willems, Martijn Beukers, Guy C M Grinwis, Saskia G M Plomp, Clemens Bos, Maarten van Dijk, Mike de Leeuw, Laura B Creemers, Marianna A Tryfonidou, Björn P Meij
Chronic low back pain is a common clinical problem in both the human and canine population. Current pharmaceutical treatment often consists of oral anti-inflammatory drugs to alleviate pain. Novel treatments for degenerative disc diseases focus on local application of sustained released drug formulations. The aim of this study was to determine safety and feasibility of intradiscal application of a PCLA-PEG-PCLA hydrogel releasing celecoxib, a COX-2 inhibitor. Biocompatibility was evaluated after subcutaneous injection in mice and safety of intradiscal injection of the hydrogel was evaluated in experimental dogs with early spontaneous intervertebral disc (IVD) degeneration...
May 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28513058/pro-myogenic-and-low-oxygen-culture-increases-expression-of-contractile-smooth-muscle-markers-in-human-fibroblasts
#4
Matija Veber, David Dolivo, Marsha Rolle, Tanja Dominko
Smooth muscle cells are essential for tissue engineering strategies to fabricate organs such as blood vessels, esophagus, and bladder, and to create disease models of these systems. In order for such therapies and models to be feasible, smooth muscle cells must be sourced effectively to enable production of large numbers of functional cells. In vitro, smooth muscle cells divide slowly and demonstrate short proliferative lifespans compared to other types of cells including stem cells and fibroblasts, limiting the number of cells that can be derived from expansion in culture of a primary isolation...
May 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28512850/in-situ-handheld-3d-bioprinting-for-cartilage-regeneration
#5
Claudia Di Bella, Serena Duchi, Cathal D O'Connell, Romane Blanchard, Cheryl Augustine, Zhilian Yue, Fletcher Thompson, Christopher Richards, Stephen Beirne, Carmine Onofrillo, Sebastien H Bauquier, Stewart D Ryan, Peter Pivonka, Gordon G Wallace, Peter F Choong
Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ 3D printing is an exciting and innovative bio-fabrication technology that enables the surgeon to deliver tissue- engineering techniques at the time and location of need. We have created a hand- held 3D printing device (Biopen) that allows the simultaneous co-axial extrusion of bioscaffold and cultured cells directly into the cartilage defect in vivo in a single session surgery. This pilot study assesses the ability of the Biopen to repair a full thickness chondral defect and the early outcomes in cartilage regeneration, and compares these results to other treatments in a large animal model...
May 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28513101/industrialization-of-a-perfusion-bioreactor-prime-example-of-a-non-straightforward-process
#6
G Talò, C Turrisi, C Arrigoni, C Recordati, I Gerges, M Tamplenizza, A Cappelluti, S A Riboldi, M Moretti
Bioreactors are essential enabling technologies for the translation of Advanced Therapies Medicinal Products (ATMPs) from the research field towards a successful clinical application. In order to speed up the translation and the spreading of novel Tissue Engineering (TE) products into the clinical routine, TE bioreactors should evolve from laboratory prototypes towards industrialized products. In this work, we thus challenged the industrialization process of a novel technological platform, based on an established research prototype of perfusion bioreactor, following a GMP-driven approach...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28512922/effect-of-chemical-immobilization-of-sdf-1%C3%AE-into-muscle-derived-scaffolds-on-angiogenesis-and-muscle-progenitor-recruitment
#7
Sareh Rajabi-Zeleti, Sasan Jalili-Firoozinezhad, Mohammad Kazemi Ashtiani, Gilles Le Carrou, Shahragim Tajbakhsh, Hossein Baharvand
The availability of three-dimensional bioactive scaffolds with enhanced angiogenic capacity that have the capability to recruit tissue specific resident progenitors is of great importance for the regeneration of impaired skeletal muscle. Here we have investigated whether introduction of chemoattractant factors to tissue specific extracellular matrix (ECM) promotes cellular behavior in vitro as well as muscle progenitor recruitment and vascularization in vivo. We developed an interconnective macroporous sponge from decellularized skeletal muscle with maintained biochemical traits of the intact muscle...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28511287/generation-and-characterization-of-highly-purified-canine-schwann-cells-from-spinal-nerve-dorsal-roots-as-potential-new-candidates-for-transplantation-strategies
#8
Nicole Steffensen, Annika Lehmbecker, Ingo Gerhauser, Yimin Wang, Regina Carlson, Andrea Tipold, Wolfgang Baumgärtner, Veronika M Stein
Schwann cells (SCs) are promising candidates for transplantation-strategies in the central nervous system by promoting axonal regeneration. The dog represents a translational model for human spinal cord injury (SCI) for studies with new repair strategies after intervertebral disk herniation (IVDH). To overcome the necessity for an additional surgical procedure, for the first time a protocol for isolation, and purification of canine SCs from spinal-nerve-biopsies during standard hemilaminectomy in IVDH affected paraplegic dogs for potential transplantation was developed...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28509443/hemodynamically-stimulated-and-in-vivo-generated-axially-vascularized-soft-tissue-free-flaps-for-closure-of-complex-defects-evaluation-in-a-small-animal-model
#9
Volker J Schmidt, Johanna O Wietbrock, Nico Leibig, Jochen F Hernekamp, Dominic Henn, Christian A Radu, Ulrich Kneser
The arteriovenous (AV) loop model permits the creation of significant volumes of axially vascularized tissue that represents an alternative to conventional free flaps, circumventing their common limitations. However, such AV-loop-based flaps have never before been examined in standardized animal models with respect to their suitability for reconstruction of critical bone exposing defects. In the course of our preliminary studies, we implemented a novel defect model in rats that provides standardized and critical wound conditions and evaluated whether AV-loop-generated flaps are suitable for free microsurgical transfer and closure of composite defects...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28509340/prf-based-matrices-to-improve-angiogenesis-in-an-in-vitro-co-culture-model-for-bone-tissue-engineering
#10
Eva Dohle, Karima El Bagdadi, Robert Sader, Joseph Choukroun, C James Kirkpatrick, Shahram Ghanaati
In the context of prevascularization strategies for tissue engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence repair processes like wound healing or angiogenesis. The development of autologous injectable platelet rich fibrin (PRF), which can be generated from peripheral blood in a minimal invasive procedure, fulfils several requirements for clinically applicable cell-based tissue engineering strategies...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28508565/menaquinone-4-enhances-osteogenic-potential-of-human-amniotic-fluid-mesenchymal-stem-cells-cultured-in-a-2d-and-3d-dynamic-culture-system
#11
Domitilla Mandatori, Letizia Penolazzi, Caterina Pipino, Pamela Di Tomo, Sara Di Silvestre, Natalia Di Pietro, Sara Trevisani, Marco Angelozzi, Mariangela Ucci, Roberta Piva, Assunta Pandolfi
Menaquinones, also known as Vitamin K2 family, regulate calcium homeostasis in a "bone-vascular cross talk" and recently received particular attention for their positive effect on bone formation. Since the correlation between menaquinones and bone metabolism to date is still unclear, the objective of our study was to investigate the possible role of menaquinone-4 (MK-4), an isoform of the menaquinones family, in the modulation of osteogenesis. For this reason we used a model of human Amniotic Fluid Mesenchymal Stem Cells (hAFMSCs) cultured both in two-dimensional (2D) and three-dimensional (3D, RCCS™bioreactor) in vitro culture systems...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28508555/laminin-and-collagen-iv-inclusion-in-immunoisolating-microcapsules-reduces-cytokine-mediated-cell-death-in-human-pancreatic-islets
#12
Alberto Llacua, Bart J de Haan, Paul de Vos
Extracellular matrix (ECM) molecules have several functions in pancreatic islets including provision of mechanical support and prevention of cytotoxicity during inflammation. During islet isolation, ECM-connections are damaged and are not restored after encapsulation and transplantation. Inclusion of specific combinations of collagen type IV and laminins in immunoisolating capsules can enhance survival of pancreatic islets. Here we investigated whether ECM can also enhance survival and lower susceptibility of human islets to cytokine-mediated cytotoxicity...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28508471/needleless-emulsion-electrospinning-for-the-regulated-delivery-of-susceptible-proteins
#13
Matej Buzgo, Eva Filova, Andrea Mickova Staffa, Michala Rampichova, Miroslav Doupnik, Karolina Vocetkova, Vera Lukasova, Radka Kolcun, David Lukas, Alois Necas, Evzen Amler
In the present work, we developed a novel needleless emulsion electrospinning technique which improves the production rate of the core/shell production process. The nanofibers are based on PCL as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibers show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate diverse set of compounds to the core phase (i.e. 5-DTAF-PF68, horseradish peroxidase (HRP), TRITC-dextran, insulin growth factor I (IGF-I), transforming growth factor beta (TGF-beta) and basic fibroblast growth factor (bFGF))...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28500666/bioactivity-and-mineralization-of-natural-hydroxyapatite-from-cuttlefish-bone-and-bioglass%C3%A2-co-sintered-bioceramics
#14
Natascia Cozza, Felipe Monte, Walter Bonani, Pranesh Aswath, Antonella Motta, Claudio Migliaresi
In this study, bioactive hydroxyapatite-based bioceramics starting from cuttlefish bone powders have been prepared and characterized. In particular, fragmented cuttlefish bone was co-sintered with 30 wt% of Bioglass®-45S5 to synthesize hydroxyapatite-based powders with enhanced mechanical properties and bioactivity. Commercial synthetic hydroxyapatite was treated following the same procedure and used as a reference. The structure and composition of the bioceramics formulations were characterized using Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy...
May 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28494141/metformin-induces-osteoblastic-differentiation-of-human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells
#15
Ping Wang, Tao Ma, Dong Guo, Kevin Hu, Yan Shu, Hockin H K Xu, Abraham Schneider
Metformin, a first-line anti-diabetic drug used by millions of patients has been shown to have potential osteogenic properties. The present study was performed to test the hypothesis that clinically relevant doses of metformin promote the osteogenic differentiation and mineralization of induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells (iPSC-MSCs). iPSC-MSCs were treated with metformin (10 μM) to assess cell viability, osteogenic differentiation, mineralization, and activation of the LKB1/AMP-activated protein kinase (AMPK) signaling pathway, a surrogate marker of metformin action...
May 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28488809/optimized-isolation-and-expansion-of-human-airway-epithelial-basal-cells-from-endobronchial-biopsy-samples
#16
Kate H C Gowers, Robert E Hynds, Ricky M Thakrar, Bernadette Carroll, Martin A Birchall, Sam M Janes
Autologous airway epithelial cells have been used in clinical tissue-engineered airway transplantation procedures with a view to assisting mucosal regeneration and restoring mucociliary escalator function. However, limited time is available for epithelial cell expansion due to the urgent nature of these interventions and slow epithelial regeneration has been observed in patients. Human airway epithelial cells can be expanded from small biopsies or brushings taken during bronchoscopy procedures but the optimal mode of tissue acquisition from patients has not been investigated...
May 10, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28488390/mesenchymal-stem-cells-protect-from-acute-liver-injury-by-attenuating-hepatotoxicity-of-liver-nkt-cells-in-inos-and-ido-dependent-manner
#17
Marina Gazdic, Bojana Simovic Markovic, Ljubica Vucicevic, Tamara Nikolic, Valentin Djonov, Nebojsa Arsenijevic, Vladimir Trajkovic, Miodrag L Lukic, Vladislav Volarevic
The effects of mesenchymal stem cells (MSCs) on phenotype and function of natural killer T (NKT) cells, is not understood. We used concanavalin A (ConA) - and α-galactosylceramide (α-GalCer)-induced liver injury to evaluate effects of MSCs on NKT-dependent hepatotoxicity. Mouse MSCs (mMSCs) significantly reduced Con A- and α-GalCer-mediated hepatitis in C57Bl/6 mice, as demonstrated by histopathological and biochemical analysis, attenuated influx of inflammatory (T-bet(+) TNF-α, IFN-γ producing and GATA3(+) , IL-4-producing) liver NKT cells and down-regulated TNF-α, IFN-γ and IL-4 levels in the sera...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28488350/cranial-reconstruction-using-allogeneic-mesenchymal-stromal-cells-a-phase-1-first-in-human-trial
#18
David Anthony Morrison, Alan Matthew Kop, Anastasia Nilasaroya, Marian Sturm, Kathryn Shaw, Stephen Honeybul
Cranioplasty is necessary for patients that have undergone craniectomy following trauma, stroke or other causes of elevated intracranial pressure. This study assessed the effectiveness of treating cranial defects with a combination of allogeneic mesenchymal stromal cells (MSC) on a ceramic carrier and polymer scaffold, to produce viable bone and healing of a cranial void. Patients underwent a baseline CT scan for construct design. Two sets of interlocking moulds were 3D-printed to enable shaping of two polymer meshes which formed the boundaries of the construct corresponding to restoration of the skull interna and externa...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28488318/platelet-rich-plasma-enhances-the-integration-of-bio-engineered-cartilage-with-native-tissue-in-an-in-vitro-model
#19
Corey Sermer, Rita Kandel, Jesse Anderson, Mark Hurtig, John Theodoropoulos
Current therapies for cartilage repair can be limited by an inability of the repair tissue to integrate with host tissue. Thus there is interest in developing approaches to enhance integration. We have previously shown that platelet-rich plasma (PRP) improves cartilage tissue formation. This raised the question as to whether PRP could promote cartilage integration. Chondrocytes were isolated from cartilage harvested from bovine joints, seeded on a porous bone substitute, and grown in vitro to form an osteochondral-like implant...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28486786/local-delivery-of-fingolimod-from-3-d-scaffolds-impacts-islet-graft-efficacy-and-microenvironment-in-a-murine-diabetic-model
#20
Anthony W Frei, Ying Li, Kaiyuan Jiang, Peter Buchwald, Cherie L Stabler
The local delivery of immunosuppressive agents could significantly promote the success of islet transplantation for the treatment of Type 1 diabetes. Fingolimod, a clinically-approved sphingosine-1-phosphate receptor agonist, has been found to dampen allograft islet rejection in rodent models when delivered systemically. Herein, we engineered a platform for the local delivery of fingolimod by incorporating it within a macroporous polydimethylsiloxane (PDMS) scaffold specifically designed for islet transplantation...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
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