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Journal of Tissue Engineering and Regenerative Medicine

D Könnig, A Herrera, G N Duda, A Petersen
In tissue defects, cells face distinct mechanical boundary conditions, but how this influences early stages of tissue regeneration remains largely unknown. Biomaterials are used to fill defects but also to provide specific mechanical or geometrical signals. However, they might at the same time shield mechanical information from surrounding tissues that is relevant for tissue functionalisation. In this study we investigated how fibroblasts in a soft macroporous biomaterial scaffold respond to distinct mechanical environments while they form microtissues...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Riccardo Coletta, Neil A Roberts, Michael J Randles, Antonino Morabito, Adrian S Woolf
An ex vivo experimental strategy that replicates in vivo intestinal development would in theory provide an accessible setting with which to study normal and dysmorphic gut biology. We recently described a system in which mouse embryonic jejunal segments were explanted onto semipermeable platforms and fed with chemically defined serum-free media. Over three days in organ culture, explants formed villi and they began to undergo spontaneous peristalsis. As defined in the current study, the wall of the explanted gut failed to form a robust longitudinal smooth muscle (SM) layer as it would do in vivo over the same time period...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Joris A van Dongen, A Jorien Tuin, Maroesjka Spiekman, Johan Jansma, Berend van der Lei, Martin C Harmsen
INTRODUCTION: Intraoperative application of stromal vascular fraction (SVF) of adipose tissue, requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) which still contains the extracellular matrix. METHODS: Pubmed, EMBASE and The Cochrane Central Register of controlled trials databases were searched for studies that compare procedures for intraoperative isolation of SVF (searched 28(th) of September, 2016)...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Khalid Algarrahi, Debra Franck, Vivian Cristofaro, Xuehui Yang, Alyssa Savarino, Saif Affas, Frank-Mattias Schäfer, Chiara Ghezzi, Russell Jennings, Arthur Nedder, David L Kaplan, Maryrose P Sullivan, Carlos R Estrada, Joshua R Mauney
Partial circumferential, full thickness defects of the esophagus can occur as a result of organ perforation, tumor resection, or during surgical reconstruction of strictured segments. Complications associated with autologous tissue flaps conventionally utilized for defect repair necessitate the development of new graft options. In this study, bi-layer silk fibroin (BLSF) scaffolds were investigated for their potential to support functional restoration of partial circumferential defects in a porcine model of esophageal repair...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
N Nomikou, G A Feichtinger, S Saha, S Nuernberger, P Heimel, H Redl, A P McHale
Gene-activated matrix (GAM)-based therapeutics for tissue regeneration are limited by efficacy, the lack of spatiotemporal control and availability of target cells, all of which impact negatively on their translation to the clinic. Here we describe an advanced ultrasound-responsive GAM containing target cells that facilitates matrix-assisted sonoporation (MAS) to induce osteogenic differentiation. Ultrasound-responsive GAMs consisting of fibrin/collagen hybrid-matrices containing microbubbles, bone morphogenetic protein BMP2/7 co-expression plasmids together with C2C12 cells were treated with ultrasound either in vitro or following parenteral intramuscular implantation in vivo...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
A Chevrier, V Darras, G Picard, M Nelea, D Veilleux, M Lavertu, C D Hoemann, M D Buschmann
The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair. A systematic approach to adjust formulation parameters including chitosan number average molar mass (Mn ), chitosan concentration and lyoprotectant concentration was undertaken to identify compositions that would rapidly (<1 minute) and completely solubilize in PRP, have paste-like handling properties upon solubilization and coagulate rapidly (<5 minutes) to form solid chitosan-PRP hybrid implants that are stable and homogenous...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Eliane Hesse, Uwe Freudenberg, Thomas Niemietz, Carina Greth, Melanie Weisser, Sébastien Hagmann, Marcus Binner, Carsten Werner, Wiltrud Richter
Cell-based tissue engineering is a promising approach for treating cartilage lesions, but available strategies still provide a distinct composition of the extracellular matrix and an inferior mechanical property compared to native cartilage. To achieve fully functional tissue replacement more rationally designed biomaterials may be needed, introducing bioactive molecules which modulate cell behavior and guide tissue regeneration. This study aimed at exploring the impact of cell instructive, adhesion (GCWGGRGDSP called RGD) and collagen-binding (CKLER/CWYRGRL) peptides, incorporated in a tunable, matrixmetalloprotease (MMP)-responsive multi-arm poly(ethylene glycol) (starPEG)/heparin hydrogel on cartilage regeneration parameters in vitro and in vivo...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Alexander J Engler, Andrew V Le, Pavlina Baevova, Laura E Niklason
In cellular, tissue-level, or whole organ bioreactors, the level of dissolved oxygen is one of the most important factors requiring control. Hypoxic environments may lead to cellular apoptosis, while hyperoxic environments may lead to cellular damage or dedifferentiation, both resulting in loss of overall tissue function. This manuscript describes the creation, characterization, and validation of a bioreactor system that can control oxygen delivery based on real-time metabolic demand of cultured whole lung tissue...
January 13, 2017: Journal of Tissue Engineering and Regenerative Medicine
Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Premasangery Kathirvaloo, Sabri Musa, Noor Hayaty Abu Kasim
Parkinson's disease (PD) is characterized by tremors and cognitive issues, and is due to the death of dopaminergic (DA-ergic) neurons in brain circuits that are responsible for producing neurotransmitter dopamine (DA). Currently, cell replacement therapies are underway to overcome existing therapeutic approaches such as drug treatments and electrical stimulation. Among the widely available sources, dental pulp stem cells from deciduous teeth (DPSC) have gained popularity due to their neural crest origin and inherent propensity toward neuronal lineage...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Yvonne Peck, Li Ting Leom, Pei Fen Patricia Low, Dong-An Wang
Rheumatoid arthritis (RA) is a chronic inflammatory disease that leads to progressive joint destruction. To further understand the process of rheumatoid cartilage damage, an in vitro model consisting of an interactive tri-culture of synovial fibroblasts (SFs), LPS-stimulated macrophages and a primary chondrocyte-based tissue engineered construct was established. The tissue engineered construct has a similar composition to human cartilage, which are rich in collagen type II and proteoglycans. Data generated from this model revealed that healthy chondrocytes were activated in the presence of SFs and macrophages...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Alexander Köwitsch, Guoying Zhou, Thomas Groth
The characteristic molecular composition of the different glycosaminoglycans (GAG) is related to their role as structural components and regulators of a multitude of functions of proteins, cells and tissues in the human body. Therefore, it is not surprising that GAG are widely used as coating materials for implants, components of 3D-constructs like tissue engineering scaffolds and hydrogels, but also as diagnostic devices like biosensors and in controlled release applications. Beside a physisorption or encapsulation of GAG, these applications often require their chemical modification to allow a stable covalent attachment on surfaces or cross-linking reactions with other molecules...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Kangquan Shou, Yao Huang, Baiwen Qi, Xiang Hu, Zhanjun Ma, Ang Lu, Chao Jian, Lina Zhang, Aixi Yu
Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered as a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder their application. Few studies report the induction of BMSCs differentiation into the skin regeneration related cell types using natural biopolymer, e.g., chitin and its derivative. Here we utilized a chitin nanofibers(CNFs) hydrogel as a directive cue to induce BMSCs differentiation for enhancing cutaneous wound regeneration in the absence of cell-differentiating factors...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
I Garzón, M A Martin-Piedra, V Carriel, M Alaminos, X Liu, R D Souza
Regeneration of the pulp-dentin complex with stem cells is a potential alternative to conventional root canal treatments. Human dental pulp stem cells (hDPSCs) have been extensively studied because of their ability to proliferate and differentiate into mineralized dental and nondental tissues. In this work we combined hDPSCs with two types of injectable poly-L-lactic acid (PLLA) microspheres with a nanofibrous or smooth surface to form bioactive injectable aggregates, and examined their ability to promote pulp regeneration in the root canal in an in vivo model...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Ivana Strazic Geljic, Nicolas Melis, Florian Boukhechba, Sébastien Schaub, Charlotte Mellier, Pascal Janvier, Jean-Pierre Laugier, Jean-Michel Bouler, Elise Verron, Jean-Claude Scimeca
Calcium phosphate-based (CaP) biomaterials are commonly used in bone reconstructive surgery to replace the damaged tissue, and can also serve as vectors for local drug delivery. Due to its inhibitory action on osteoclasts, the semi-metallic element gallium (Ga) is used for the systemic treatment of disorders associated with accelerated bone resorption. As it was demonstrated that Ga could be incorporated in the structure of CaP biomaterials, we investigated in the present work the biological properties of Ga-loaded CaP biomaterials...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Heidi R H Supanc, Shannon Gorman, Rocky S Tuan
Traumatized muscle, such as that debrided from blast injury sites, is considered a promising and convenient tissue source for multipotent progenitor cells (MPCs), a population of adult mesenchymal stem cell (MSC)-like cells. The present study aimed to assess the regenerative therapeutic potential of human traumatized muscle-derived MPCs, e.g., for injury repair in the blast-traumatized extremity, by comparing their pro-angiogenic potential in vitro and capillary recruitment activity in vivo to those of MSCs isolated from human bone marrow, a widely-used tissue source...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
Alexander Assmann, Marc Struß, Franziska Schiffer, Friederike Heidelberg, Hiroshi Munakata, Elena V Timchenko, Pavel E Timchenko, Tim Kaufmann, Khon Huynh, Yukiharu Sugimura, Quentin Leidl, Antonio Pinto, Volker R Stoldt, Artur Lichtenberg, Payam Akhyari
Low immunogenicity and high repopulation capacity are crucial determinants for the functional and structural performance of acellular cardiovascular implants. The present study evaluates a detergent-free, non-proteolytic, actin-disassembling regimen (BIO) for decellularization of heart valve and vessel grafts, particularly focusing on their bio-functionality. Rat aortic conduits (rAoC; n = 89) and porcine aortic valve samples (n = 106) are decellularized using detergents (group DET) or the BIO regimen...
January 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
Elizabeth E Smith, Weibo Zhang, Nathan R Schiele, Ali Khademhosseini, Catherine K Kuo, Pamela C Yelick
A long-term goal is to bioengineer, fully functional, living teeth for regenerative medicine and dentistry applications. Biologically based replacement teeth would avoid insufficiencies of the currently used dental implants. Using natural tooth development as a guide, a model was fabricated using post-natal porcine dental epithelial (pDE), porcine dental mesenchymal (pDM) progenitor cells, and human umbilical vein endothelial cells (HUVEC) encapsulated within gelatin methacrylate (GelMA) hydrogels. Previous publications have shown that post-natal DE and DM cells seeded onto synthetic scaffolds exhibited mineralized tooth crowns composed of dentin and enamel...
January 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
Catherine G Y Ngan, Anita Quigley, Robert M I Kapsa, Peter F M Choong
No abstract text is available yet for this article.
January 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
Anni Sorkio, Suvi Haimi, Vincent Verdoold, Kati Juuti-Uusitalo, Dirk Grijpma, Heli Skottman
Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell therapies show tremendous potential for the treatment of retinal degenerative diseases. A tissue engineering approach, where cells are delivered to the subretinal space on a biodegradable carrier as a sheet, shows great promise for these RPE cell therapies. The aim of the present study was to assess whether a flexible, elastic and biodegradable poly(trimethylene carbonate) (PTMC) film promotes the formation of functional hESC-RPE and performs better than often used biodegradable poly(d,l-lactide) (PDLLA) film...
January 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
Y H Martin, K Jubin, S Smalley, J P F Wong, R A Brown, A D Metcalfe
Cell therapy with autologous or allogeneic keratinocytes applied as a single-cell suspension is well established in clinical practice in the treatment of severe burn injuries to augment epithelial barrier restoration. Yet, the application of cell sprays can lead to significant cell loss owing to lack of adhesion of cell suspension to the wound bed. The development of a robust and controllable method of transplanting cells onto the wound bed is yet to be established. The ability to control adhesion and distribution of cells by using a cell carrier embedded in a biodegradable scaffold could significantly improve the treatment of cutaneous wounds with keratinocyte cell therapy...
January 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
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