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Journal of Tissue Engineering and Regenerative Medicine

Ahmad Abu-Hakmeh, Allison K M Fleck, Leo Q Wan
Fibroblast-like synoviocytes (FLS) are major contributors to the composition and function of synovial fluid (SF). In disease, changes to important SF molecules such as hyaluronic acid (HA), lubricin, and numerous inflammatory markers contribute to a loss of SF functional properties. Previous studies characterized the ability of FLS to produce SF molecules in short term cultures using continuous cytokine supplementation. This study assessed the HA, lubricin, and matrix metalloproteinase-2 (MMP-2) secretion profile of FLS over 12 days of culture...
November 7, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yangzi Tian, Richard Davis, Michael R Zonca, Jeff Stern, Sally Temple, Yubing Xie
Retinal pigment epithelial (RPE) cells are highly specialized neural cells that have several functions essential for vision. Progressive deterioration of RPE cells in elderly individuals can result in visual impairment and ultimately the blinding disease age-related macular degeneration (AMD). Subretinal transplantation of stem-cell-derived RPE cell suspensions is being explored as a strategy to recover the damaged retina and improve vision. This approach may be improved by developing a vehicle that increases post-injection cell viability, distribution and integration of RPE cells...
October 31, 2018: Journal of Tissue Engineering and Regenerative Medicine
Dina G Moussa, Conrado Aparicio
More than two-thirds of the global population suffers from tooth decay, which results in cavities with various levels of lesion severity. Clinical interventions to treat tooth decay range from simple coronal fillings to invasive root canal treatment. Pulp capping is the only available clinical option to maintain the pulp vitality in deep lesions, but irreversible pulp inflammation and re-infection are frequent outcomes for this treatment. When affected pulp involvement is beyond repair, the dentist has to perform endodontic therapy leaving the tooth non-vital and brittle...
October 30, 2018: Journal of Tissue Engineering and Regenerative Medicine
Patrick A Link, Alexandria M Ritchie, Gabrielle M Cotman, Michael S Valentine, Bret S Dereski, Rebecca L Heise
Decellularized extracellular matrix (ECM) is an effective tissue repair scaffold. Additionally, ECM has recently been shown to be protective to the lungs. However, current processing is inadequate for effective delivery of ECM to the lungs. Processing methods, such as milling, produce large variability in particle sizes. The size variation produced is ineffective at treating the lung because only a small range of sizes reach the distal regions of alveoli. The aim of this work is to formulate decellularized ECM to reach the distal lung, while retaining the pro-regenerative effects of ECM...
October 26, 2018: Journal of Tissue Engineering and Regenerative Medicine
Arantxa Blázquez-Prunera, Catarina R Almeida, Mario A Barbosa
Human mesenchymal stem cells (MSC) are being explored for cell therapies targeting varied human diseases. For that, cells are being expanded in vitro, many times with fetal bovine serum (FBS) as the main source of growth factors. However, animal-derived components should not be used, to avoid immune rejection from the patient that receives the MSC. To solve this issue, different xeno-free media are being developed and an industrial-grade human plasma fraction (SCC) is a promising candidate to substitute FBS...
October 26, 2018: Journal of Tissue Engineering and Regenerative Medicine
Ulrich Zwirner, Klaus Höffler, Michael Pflaum, Sotirios Korossis, Axel Haverich, Bettina Wiegmann
INTRODUCTION: Several key prerequisites need to be fulfilled for the development of a biohybrid lung which can offer an actual alternative to lung transplantation. A major aspect is an optimized hemocompatibility of the device's artificial surfaces via endothelial cell seeding. In this study, four different types of polymeric gas exchange hollow fiber membranes (HFMs) were analyzed utilizing four different seeding protocols in order to identify the ideal combination for sufficient long-term endothelialization...
October 25, 2018: Journal of Tissue Engineering and Regenerative Medicine
Rupnarayan Bhattacharya, Piyali Das, Siddhartha Narayan Joardar, Bikash Kanti Biswas, Subhasis Batabyal, Pradip Kumar Das, Samit Kumar Nandi
Restoration of the external ear and nose in human patients, in either congenital deformity or acquired defects, is a challenge in reconstructive surgery. Optimization of the currently available materials is necessary for rhinoplasty and microtia correction to avoid intraoperative manoeuvring and early rejection. The aim of this study is to develop cross-linked decellularized caprine conchal cartilages as biocompatible, robust and non-toxic matrix template. The characterization of the decellularized tissue encompasses in vitro lymphoproliferation assay, cytotoxicity test, agar gel precipitation test, in vivo immunocompatibility study, histology, and determination of pro-inflammatory cytokines in animal model...
October 24, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yusuke Sakai, Makiko Koike, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Tamotsu Kuroki, Susumu Eguchi
Subcutaneous transplantation of engineered hepatocyte/fibroblast sheets (EHFSs) is a low invasive and safe approach to construct vascularized subcutaneous human liver tissue (VSLT). However, the liver-specific structures and functionalities in the development process of VSLTs in mice remains poorly understood. Here we describe time-dependent characteristics of the formation of the vascular network, cell-cell adhesions, liver transporters, liver-specific protein synthesis, and metabolizing activities. The EHFSs formed multi-layered thick tissues by rapid neovascularization allows overcoming extremely difficult problems, such as the lack of oxygen supply on the formation of three-dimensional primary hepatocyte tissue under the skin...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Sorour Nemati, Hamed Alizadeh Sardroud, Ali Baradar Khoshfetrat, Majid Khaksar, Mahdi Ahmadi, Hassan Amini, Shirin Saberianpour, Aref Delkhosh, Ali Akbar Movassaghpour, Reza Rahbarghazi
Today, many attempts have been collected in the field of tissue engineering for reconstitution of injured bone marrow capacity by transplantation of functional cell source. By having a 3D condition, microcapsules are appropriate candidates for cells transplantation to target sites. Here, we examined the effect of alginate-gelatin microcapsules on functional maturation of human myelomonocytic cell line U937 after 7 days in vitro. U937 cells were encapsulated by the mixture of alginate-gelatin and cultured for 7 days...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Xiaoxiang Li, Nima Saeidi, Martin Villiger, Hassan Albadawi, Jake D Jones, Kyle P Quinn, William G Austin, Alexander Golberg, Martin L Yarmush
The demand for skin rejuvenation procedures has progressively increased in the past decade. Additionally, clinical trials have shown that current therapies might cause downtime and side effects in patients including prolonged erythema, scarring, and dyspigmentation. The goal of this study was to explore the effect of partial irreversible electroporation (pIRE) with pulsed electric fields in aged skin rejuvenation as a novel, noninvasive skin resurfacing technique. In this study, we used an experimental model of aged rats...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Rachel C Nordberg, Hao Wang, Qingyu Wu, Elizabeth G Loboa
Corin has been studied extensively within the vascular system, and is known to regulate blood pressure. We have shown that corin is one of the most highly upregulated genes during osteogenic differentiation of human adipose derived stem cells (hASC). This study tested the hypothesis that, through modulation of angiogenic signaling pathways, corin is a critical regulator of osteogenic differentiation and endochondral ossification. In vitro, corin expression in hASC was suppressed via siRNA knockdown and vascular endothelial growth factor A (VEGF-A) expression was quantified via RT-PCR...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Tsz Kin Ng, Qichen Yang, Veronica R Fortino, Nikky Yuk-Ki Lai, Carlos M Carballosa, Jordan M Greenberg, Kwong Wai Choy, Daniel Pelaez, Chi Pui Pang, Herman S Cheung
Neurogenesis is the basis of stem cell tissue engineering and regenerative medicine for central nervous system (CNS) disorders. We have established differentiation protocols to direct human periodontal ligament-derived stem cells (PDLSCs) into neuronal lineage, and we recently isolated the neural crest sub-population from PDLSCs, which are pluripotent in nature. Here we report the neural differentiation potential of these periodontal ligament-derived neural crest stem cells (NCSCs) as well as its microRNA (miRNA) regulatory mechanism and function in NCSC neural differentiation...
October 23, 2018: Journal of Tissue Engineering and Regenerative Medicine
Timothy Kamaldinov, Josh Erndt-Marino, Patricia Diaz-Rodriguez, Hongyu Chen, Tanmay Gharat, Dany Munoz-Pinto, Brigitte Arduini, Mariah S Hahn
Clinical use of human embryonic stem cells (hESCs) in bone regeneration applications requires that their osteogenic differentiation be highly controllable as well as time and cost effective. The main goals of the current work were thus: 1) to assess whether overexpression of pluripotency regulator Forkhead Box D3 (FOXD3) can enhance the osteogenic commitment of hESCs seeded in a 3D scaffolds, and 2) to evaluate if the degree of FOXD3 overexpression regulates the strength specificity of hESC osteogenic commitment...
October 22, 2018: Journal of Tissue Engineering and Regenerative Medicine
Jing Liu, Chengpan Li, Shaohui Cheng, Shengnan Ya, Dayong Gao, Weiping Ding
In vitro engineering of liver tissue is a rapidly developing field for various biomedical applications. However, liver tissue culture is currently performed on only a small scale with a low density of hepatocytes. In this study, a simple design was introduced in a liver microsystem to enhance the transport of nutrients (e.g., oxygen and glucose) for the 3D large-scale, high-density culture of hepatocytes. In this design, convection across the cell culture zone was generated to mimic sinusoid blood flow (SBF) based on the pressure difference between two fluids flowing in a countercurrent manner on either side of the cell culture zone...
October 22, 2018: Journal of Tissue Engineering and Regenerative Medicine
Arnold V Popkov, Dimitry A Popkov, Natalia A Kononovich, Elena N Gorbach, Sergei I Tverdokhlebov, Evgeny N Bolbasov, Evgeniy O Darvin
Early treatment of bone fractures was performed using implants, which are often used in the form of plates of various types, which are fixed on the bone surface (extracellular fixation) and nails that are located in the medullary canal (intracerebral fixation). The goal of this study was to investigate the features of osseointegration of implants for internal fixation (intramedullar or extramedular) with various bioactive coating techniques. During experimental study on 20 mongrel dogs, the implant model in the form of 1...
October 22, 2018: Journal of Tissue Engineering and Regenerative Medicine
Sergio Casadiegos, Rosa Helena Bustos, Marta R Fontanilla
Analysis of factors that play a role on the healing process in exudates from skin wounds might shed light on the effect that grafted artificial tissue has in wound regeneration and repair. The first objective of this work was to standardize an optic surface plasmon resonance method based on self-assembled monolayers to quantify healing mediator factors (Angiopoietin-2, Epidermal Growth Factor, Tumor Necrosis Factor-α, Transforming Growth Factor-β1, and Vascular Endothelial Growth Factor) in wound exudates...
October 22, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yoji Jokura, Kazuo Yano, Masayuki Yamato
No abstract text is available yet for this article.
October 18, 2018: Journal of Tissue Engineering and Regenerative Medicine
Ingra Mannhardt, Christoph Warncke, Hoc Khiem Trieu, Jörg Müller, Thomas Eschenhagen
BACKGROUND: Engineered heart tissue (EHT) has proven as valuable tool for disease modeling, drug safety screening and cardiac repair. Especially in combination with the stem cell technology, these in vitro models of the human heart have generated interest not only of basic cardiovascular researchers, but also regulatory authorities responsible for drug safety. A main limitation of 3D-based assays for evaluating cardiotoxicity is their limited throughput. METHODS: We integrated piezo-bending actuators in a 24-well system for the generation of strip-like rat and human EHT attached to hollow, elastic silicone posts...
October 18, 2018: Journal of Tissue Engineering and Regenerative Medicine
Niamh O' Halloran, Eimear Dolan, Michael Kerin, Aoife Lowery, Garry Duffy
Current methods of breast reconstruction are associated with significant shortcomings, including capsular contracture, infection, rupture, the need for reoperation in implant-based reconstruction, and donor site morbidity in autologous reconstruction. These limitations result in severe physical and psychological issues for breast cancer patients. Recently, research has moved into the field of adipose tissue engineering to overcome these limitations. A wide range of regenerative strategies have been devised utilising various scaffold designs and biomaterials...
October 18, 2018: Journal of Tissue Engineering and Regenerative Medicine
Dong-Kyu Kim, Jung Min Lee, Ju Yeon Jeong, Hyun Jung Park, Ok Joo Lee, Janet Chao, Soon Hee Kim, Hae Sang Park, Gilson Khang, Chan Hum Park
Recently, a newer generation of absorbable biomaterials has been developed from silk. Silk is approved by the US Food and Drug Administration, has robust mechanical features, and is biocompatible. Moreover, it offers the ability to be functionalized with bioactive compounds, making it ideal for use in new medical devices. Thus, many researchers have considered that absorbable devices made from silk may be able to overcome current limitations and could be used to meet a broader range of fixation needs. Here, we describe a novel method for the fabrication of silk fibroin (SF)-based bioabsorbable fixation systems using a centrifugal casting technique and incorporating a 3D printer...
September 28, 2018: Journal of Tissue Engineering and Regenerative Medicine
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