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Journal of Tissue Engineering and Regenerative Medicine

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https://www.readbyqxmd.com/read/29330944/improvement-of-islet-function-and-survival-by-integration-of-perfluorodecalin-into-microcapsules-in-vivo-and-in-vitro
#1
Sang-Ho Lee, Heon-Seok Park, Yeoree Yang, Eun-Young Lee, Ji-Won Kim, Gilson Khang, Kun-Ho Yoon
Hypoxic injury of islets is a major obstacle for encapsulated islet transplantation into the peritoneal cavity. To improve oxygen delivery to encapsulated islets, we integrated 20% of the oxygen carrier material, perfluorodecalin (PFD), in alginate capsules mixed with islets (PFD-alginate). Integration of PFD clearly improved islet viability and decreased reactive oxygen species production compared to islets encapsulated with alginate only (alginate) and naked islets exposed to hypoxia in vitro. In PFD-alginate capsules, HIF-1α expression was minimal, and insulin expression was well maintained...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29330921/four-and-a-half-domain-2-fhl2-scaffolding-protein-is-a-marker-of-connective-tissues-of-developing-digits-and-regulates-fibrogenic-differentiation-of-limb-mesodermal-progenitors
#2
C I Lorda-Diez, J A Montero, C Sanchez-Fernandez, J A Garcia-Porrero, J Chimal-Monroy, J M Hurle
FHL2 is a multifunctional scaffolding protein of well-known function regulating cell signaling cascades and gene transcription in cancer tissues. However, its function in embryonic systems is poorly characterized. Here, we show that Fhl2 is involved in the differentiation of connective tissues of developing limb autopod. We show that Fhl2 exhibits spatially restricted and temporally dynamic expression around the tendons of developing digits, interphalangeal joint capsules, and fibrous peridigital tissue. Immunolabeling analysis of the skeletal progenitors identified a predominant, but not exclusive, cytoplasmic distribution of FHL2 being associated with focal adhesions and actin cytoskeleton...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327452/regenerative-effects-of-human-embryonic-stem-cell-derived-neural-crest-cells-for-treatment-of-peripheral-nerve-injury
#3
Iwan Jones, Liudmila N Novikova, Lev N Novikov, Monika Renardy, Andreas Ullrich, Mikael Wiberg, Leif Carlsson, Paul J Kingham
Surgical intervention is the current gold standard treatment following peripheral nerve injury. However, this approach has limitations and full recovery of both motor and sensory modalities often remains incomplete. The development of artificial nerve grafts that either complement or replace current surgical procedures is therefore of paramount importance. An essential component of artificial grafts are biodegradable conduits and transplanted cells that provide trophic support during the regenerative process...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327431/enhanced-bone-regeneration-and-visual-monitoring-via-super-paramagnetic-iron-oxide-nanoparticle-scaffold-in-rats
#4
Shuying Hu, Yi Zhou, Yantao Zhao, Yang Xu, Feimin Zhang, Ning Gu, Junqing Ma, Mark A Reynolds, Yang Xia, Hockin H K Xu
A main challenge for use of scaffolds in bone engineering involves non-invasive monitoring in vivo and enhanced bone regeneration. The tissue-repair effect of super-paramagnetic iron oxide nanoparticles (SPIONs) was demonstrated previously by our group. However, testing in vivo is needed to confirm in vitro results. Here, SPIONs loaded gelatin sponge (GS) was used as a scaffold (SPIONs-GS) and implanted in the incisor sockets of Sprague-Dawley rats. Incisor sockets filled with nothing and filled with GS served as controls...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327429/in-vivo-migration-of-endogenous-brain-progenitor-cells-guided-by-an-injectable-peptide-amphiphile-biomaterial
#5
Reza Motalleb, Eric J Berns, Piyush Patel, Julie Gold, Samuel I Stupp, H Georg Kuhn
Biomaterials hold great promise in helping the adult brain regenerate and rebuild after trauma. Peptide amphiphiles (PA) are highly versatile biomaterials, gelling and forming macromolecular structures when exposed to physiological levels of electrolytes. We are here reporting on the first ever in vivo use of self-assembling peptide amphiphile carrying a Tenascin-C signal (E2 Ten-C PA) for the re-direction of endogenous neuroblasts in the rodent brain. The PA forms highly aligned nanofibers, displaying the migratory sequence of Tenascin-C glycoprotein as epitope...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327490/fetal-subcutaneous-cells-have-potential-for-autologous-tissue-engineering
#6
Åsa Ekblad, Magnus Westgren, Magdalena Fossum, Cecilia Götherström
BACKGROUND: Major congenital malformations affect up to 3% of newborns. Infants with prenatally diagnosed soft tissue defects should benefit from having autologous tissue readily available for surgical implantation in the perinatal period. In this study, we investigate fetal subcutaneous cells (fSC) as cellular source for tissue engineering. METHODS: Fetal subcutaneous biopsies were collected from elective terminations at gestational week 20-21. Cells were isolated, expanded and characterized in vitro...
January 11, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327436/effect-of-patterned-electrospun-hierarchical-structures-on-alignment-and-differentiation-of-mesenchymal-stem-cells-biomimicking-bone
#7
Sharanya Sankar, Manohar Kakunuri, Sindhuja D E, Chandra S Sharma, Subha N Rath
Considering the complex hierarchical structure of bone, biomimicking the micro and nano level features should be an integral part of scaffold fabrication for successful bone regeneration. We aim to biomimic the micro- and nano-structure of bone and study the effect of physical cues on cell alignment, proliferation and differentiation. To achieve this, we have divided the scaffolds into groups: electrospun SU-8 nanofibers, electrospun SU-8 nanofibers with UV treatment and micropatterned (20 μm sized ridges and grooves) SU-8 nanofibers by photolithography with UV treatment...
January 11, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29327428/an-endochondral-ossification-approach-to-early-stage-bone-repair-use-of-tissue-engineered-hypertrophic-cartilage-constructs-as-primordial-templates-for-weight-bearing-bone-repair
#8
Amos Matsiko, Emmet M Thompson, Cai Lloyd-Griffith, Gráinne M Cunniffe, Tatiana Vinardell, John P Gleeson, Daniel J Kelly, Fergal J O'Brien
Mimicking endochondral ossification to engineer constructs offers a novel solution to overcoming the problems associated with poor vascularisation in bone repair. This can be achieved by harnessing the angiogenic potency of hypertrophic cartilage. In this study, we demonstrate that tissue-engineered hypertrophically-primed cartilage constructs can be developed from collagen-based scaffolds cultured with mesenchymal stem cells. These constructs were subsequently implanted into femoral defects in rats. It was evident that the constructs could support enhanced early-stage healing at 4 weeks of these weight-bearing femoral bone defects compared to untreated defects...
January 11, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29320615/an-intervertebral-disc-whole-organ-culture-system-to-investigate-proinflammatory-and-degenerative-disc-disease-condition
#9
Gernot Lang, Yishan Liu, Janna Geries, Zhiyu Zhou, David Kubosch, Norbert Südkamp, R Geoff Richards, Mauro Alini, Sibylle Grad, Zhen Li
OBJECTIVE: The aim of this study was to compare the effect of different disease initiators of degenerative disc disease (DDD) within an intervertebral disc (IVD) organ culture system, and to understand the interplay between inflammation and degeneration in the early stage of DDD. DESIGN: Bovine caudal IVDs were cultured within a bioreactor for up to 11 days. Control group was cultured under physiological loading (0.02-0.2 MPa; 0.2 Hz; 2h/day) and high glucose (4...
January 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29314764/biomimetic-fetal-rotation-bioreactor-for-engineering-bone-tissues-effect-of-cyclic-strains-on-upregulation-of-osteogenic-gene-expression
#10
Akhilandeshwari Ravichandran, Feng Wen, Jing Lim, Mark Seow Khoon Chong, Jerry K Y Chan, Swee-Hin Teoh
Cells respond to physiological mechanical stresses especially during early fetal development. Adopting a biomimetic approach, it is necessary to develop bioreactor systems to explore the effects of physiologically relevant mechanical strains and shear stresses for functional tissue growth and development. This study introduces a multimodal bioreactor system that allows application of cyclic compressive strains on premature bone grafts that are cultured under biaxial rotation (chamber rotation about two axes) conditions for bone tissue engineering...
January 3, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29286211/characterization-of-costal-cartilage-and-its-suitability-as-a-cell-source-for-articular-cartilage-tissue-engineering
#11
Le W Huwe, Wendy E Brown, Jerry C Hu, Kyriacos A Athanasiou
Costal cartilage is a promising donor source of chondrocytes to alleviate cell scarcity in articular cartilage tissue engineering. Limited knowledge exists, however, on costal cartilage characteristics. This study describes the characterization of costal cartilage and articular cartilage properties and compares neocartilage engineered with costal chondrocytes to native articular cartilage, all within a sheep model. Specifically, we 1) quantitatively characterized the properties of costal cartilage in comparison to patellofemoral articular cartilage, and 2) evaluated the quality of neocartilage derived from costal chondrocytes for potential use in articular cartilage regeneration...
December 29, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29272079/development-of-lacrimal-gland-spheroids-for-lacrimal-gland-tissue-regeneration
#12
Isobel Massie, Kristina Spaniol, Andreas Barbian, Gerd Geerling, Marco Metzger, Stefan Schrader
Severe dry eye syndrome (DES) resulting from lacrimal gland (LG) dysfunction can cause blindness, yet treatments remain palliative. In vitro reconstruction of LG tissue could provide a curative treatment. We aimed to combine epithelial cells with endothelial cells and mesenchymal stem cells (MSCs) to form a 3D functional unit. Epithelial cells and MSCs were isolated from porcine LG, endothelial cells were isolated from human foreskin. MSCs were characterised (flow cytometry, differentiation potential assays)...
December 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29266875/collagen-nanofiber-anisotropy-induces-myotube-differentiation-and-acetylcholine-receptor-clustering
#13
Frank H Kung, David Sillitti, Anil B Shrirao, David I Shreiber, Bonnie L Firestein
To create musculoskeletal tissue scaffolds for functional integration into host tissue, myotubes must be properly aligned with native tissue and spur the formation of neuromuscular junctions (NMJs). However, our understanding of myoblast differentiation in response to structural alignment is incomplete. To examine how substrate anisotropy mediates myotube differentiation, we studied C2C12 myoblasts grown on aligned collagen substrates in the presence or absence of agrin. Myoblasts grown on microfluidically patterned collagen substrates demonstrated increased multinucleated myotubes and nicotinic acetylcholine receptor (AChR) clusters...
December 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29266858/construction-of-a-vascularized-hydrogel-for-cardiac-tissue-formation-in-a-porcine-model
#14
K P Myu Mai Ja, Kee Pah Lim, Allen Chen, Sherwin Ting, Shi Qi Li, Nicole Tee, Chrishan Ramachandra, Ashish Mehta, Philip Wong, Steve Oh, Winston Shim
Replacing cardiac tissues lost to myocardial infarction remains a therapeutic goal for regenerative therapy in recovering cardiac function. We assessed the feasibility of constructing a macro-sized human cardiac tissue construct using pluripotent stem cell-derived cardiomyocytes or control fibroblasts infused fibrin/collagen hydrogel and performed ectopic implantation in peripheral vascular system of a porcine model for 3 weeks. Finally, an optimized vascularized cardiac construct was explanted and grafted onto porcine myocardium for 2 weeks...
December 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29266822/segregation-of-motor-and-sensory-axons-regenerating-through-bicompartmental-tubes-by-combining-extracellular-matrix-components-with-neurotrophic-factors
#15
Jaume Del Valle, Daniel Santos, Ignacio Delgado-Martínez, Natàlia de la Oliva, Guido Giudetti, Silvestro Micera, Xavier Navarro
Segregation of regenerating motor and sensory axons may be a good strategy to improve selective functionality of regenerative interfaces to provide close loop commands. Provided that extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert guidance effects on different neuronal populations, we assessed in vivo the potential of separating sensory and motor axons regenerating in a bicompartmental Y-type tube, with each branch prefilled with an adequate combination of ECM and NTFs. The severed rat sciatic nerve was repaired using a bicompartmental tube filled with a collagen matrix enriched with fibronectin and BDNF encapsulated in PLGA microspheres (FN+MP...
December 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29243352/a-review-on-gradient-hydrogel-fiber-scaffolds-for-osteochondral-regeneration
#16
REVIEW
Sajedeh Khorshidi, Akbar Karkhaneh
Osteochondral tissue regeneration is a complicated field due to the distinct properties and healing potential of osseous and chondral phases. In a natural osteochondral region, the composition, mechanics and structure vary smoothly from bony to cartilaginous phase. Therefore, a homogeneous scaffold cannot satisfy the complexity of the osteochondral matrix. In essence, a natural extracellular matrix (ECM) is composed of fibrous proteins elongated into a gelatinous background. A hydrogel/fiber scaffold possessing gradient in both phases would be of the utmost interest to imitate tissue arrangement of a native osteochondral interface...
December 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222846/influence-of-the-three-dimensional-culture-of-human-bone-marrow-mesenchymal-stromal-cells-within-a-macroporous-polysaccharides-scaffold-on-pannexin-1-and-pannexin-3
#17
Julien Guerrero, Hugo Oliveira, Rachida Aid, Reine Bareille, Robin Siadous, Didier Letourneur, Yong Mao, Joachim Kohn, Joëlle Amédée
Because cell interactions play a fundamental role for cell differentiation, we investigated the expression of Pannexin 1 and Pannexin 3 in human bone marrow mesenchymal stromal cells (HBMSCs) in a three-dimensional (3D) microenvironment provided by a polysaccharide-based macroporous scaffold. The pannexin (Panx) family consists of three members, Panx1, Panx2, and Panx3. The roles of pannexin large-pore ion and metabolite channels are recognized in many physiological and pathophysiological scenarios but the role of these proteins in human physiological processes is still under investigation...
December 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222837/low-melt-bioactive-glass-reinforced-3d-printing-akermanite-porous-cages-with-highly-improved-mechanical-properties-for-lumbar-spinal-fusion
#18
Xiurong Ke, Lei Zhang, Xianyan Yang, Juncheng Wang, Chen Zhuang, Zhouwen Jin, An Liu, Tengfei Zhao, Sanzhong Xu, Changyou Gao, Zhongru Gou, Guojing Yang
Although great strides have been made in medical technology, low back/neck pain and intervertebral disc degeneration initiated from disc degenerative disease remains a clinical challenge. Within the field of regenerative medicine therapy, we have sought to improve the biomechanical transformation of spinal fusion procedures conducted using biodegradable porous implants. Specifically, we have focused on developing mechanically strong bioceramic cages for spinal fusion and functional recovery. Herein we fabricated the akermanite (AKE) ceramic-based porous cages using low-melting bioactive glass (BG) and 3D printing technology...
December 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222849/bone-marrow-transplantation-improves-motor-activity-in-a-mouse-model-of-ataxia
#19
David Díaz, Marina Piquer-Gil, Javier Sánchez Recio, María Magdalena Martínez-Losa, José Ramón Alonso, Eduardo Weruaga, Manuel Álvarez-Dolado
Ataxias are locomotor disorders that can have an origin both neural and muscular, although both impairments are related. Unfortunately, ataxia has no cure and the current therapies are aimed at motor re-education or muscular reinforcement. Nevertheless, cell therapy is becoming a promising approach to deal with incurable neural diseases, including neuro-muscular ataxias. Here we have used a model of ataxia, the PCD mutant mouse, to study the effect of healthy (wild-type) bone marrow transplantation on the restoration of defective mobility...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222841/evaluation-of-an-ovary-on-a-chip-in-large-mammalian-models-species-specificity-and-influence-of-follicle-isolation-status
#20
J Nagashima, R El Assal, N Songsasen, U Demirci
The ability to grow oocytes from immature ovarian follicles in vitro has significant potential for fertility preservation; yet, it has proved challenging in large mammalian species due to the complex metabolic needs and long-term culture requirements. Currently, follicular incubations are based on a 'static' system with manual exchange of medium. Despite the numerous advantages of conventional culturing approaches, recapitulating the native microenvironment and supporting the survival of ovarian follicles still represent challenges...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
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