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Journal of Tissue Engineering and Regenerative Medicine

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https://www.readbyqxmd.com/read/28719946/cell-assisted-lipotransfer-friend-or-foe-in-fat-grafting-systematic-review-and-meta-analysis
#1
REVIEW
J Laloze, A Varin, J Gilhodes, N Bertheuil, J L Grolleau, J Brie, J Usseglio, L Sensebe, T Filleron, B Chaput
BACKGROUND: Autologous fat grafting is a common procedure for soft-tissue reconstruction but is associated with a graft resorption rate ranging from 20% to 80%. To improve the fat graft survival rate, a new technique, called cell-assisted lipotransfer (CAL), was developed. With CAL, fat is injected along with adipose-derived stromal cells (ASCs) that are assumed to improve fat survival rate. We conducted an evidence-based meta-analysis to evaluate the efficacy and safety of CAL as compared with conventional autologous fat grafting (non-CAL)...
July 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28719734/tissue-engineering-of-a-composite-trachea-construct-using-autologous-rabbit-chondrocytes
#2
James E Dennis, Kristina G Bernardi, Thomas J Kean, Nelson E Liou, Tanya K Meyer
The repair of large tracheal segmental defects remains an unsolved problem. The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements. Engineered tracheal replacements composed of autologous cells ("neotracheas") were tested in a New Zealand White rabbit model. Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle and an engineered cartilage sheet, and allowing the construct to mature for 8-12 weeks...
July 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715614/intraperitoneal-administration-of-adipose-tissue-derived-stem-cells-for-the-rescue-of-retinal-degeneration-in-a-mouse-model-via-indigenous-cntf-up-regulation-by-il-6
#3
Jeong Hoon Heo, Jung Ae Yoon, Eun Kyung Ahn, Hyun Kim, Sang Hwa Urm, Chul Oh Oak, Byeng Chul Yu, Sang Joon Lee
As the world's population begins to age, retinal degeneration is an increasing problem, and various treatment modalities are being developed. However, there have been no therapies for degenerative retinal conditions that are not characterized by neovascularization. We investigated whether transplantation of mouse adipose tissue-derived stem cells (mADSC) into the intraperitoneal space has a rescue effect on NaIO3 -induced retinal degeneration in mice. In this study, mADSC transplantation recovered visual function and preserved the retinal outer layer structure compared to the control group without any integration of mADSC into the retina...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715156/neutrophil-mediated-enhancement-of-angiogenesis-and-osteogenesis-in-a-novel-triple-cell-co-culture-model-with-endothelial-cells-and-osteoblasts
#4
T D K Herath, Anis Larbi, Swee Hin Teoh, C James Kirkpatrick, Bee Tin Goh
Repair and regeneration of critical-sized bone defects remain a major challenge in orthopaedic and craniomaxillofacial surgery. Until now, attempts to bioengineer bone tissue have been hindered by the inability to establish proper angiogenesis and osteogenesis in the tissue construct. In the present study, we established a novel triple cell co-culture model consisting of osteoblasts, endothelial cells, and neutrophils and conducted a systematic investigation of the effects of neutrophils on angiogenesis and osteogenesis...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715143/repetitive-short-span-application-of-extracellular-calcium-is-osteopromotive-to-osteoprogenitor-cells
#5
Hyungjin Jung, Thomas Mbimba, Mustafa Unal, Ozan Akkus
Extracellular calcium ([Ca(2+) ]E ) concentration has been suggested to stimulate osteoblastic activity; thus, calcium can be used to enhance fracture healing. However, systemic administration of calcium at high dose levels may cause physiological problems such as hypercalcemia. Short span application of single or repetitive [Ca(2+) ]E stimulus may be suggested as a novel regimen to reduce such side effects. However, osteopromotive effect of such short term [Ca(2+) ]E stimulus on osteoprogenitor cells has not yet been evaluated yet...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715139/uvb-exposure-of-a-humanized-skin-model-reveals-unexpected-dynamic-of-keratinocyte-proliferation-and-wnt-inhibitor-balancing
#6
Teresa Michalczyk, Thomas Biedermann, Sophie Böttcher-Haberzeth, Agnes S Klar, Martin Meuli, Ernst Reichmann
We developed human dermo-epidermal skin substitutes which are presently applied in phase I and II clinical trials. Here we used these very same skin equivalents containing melanocytes, named MelSkin, as an experimental skin model. We investigated the effects of UVB irradiation on the skin grafts transplanted on immune-compromised rats. The irradiation induces a strong wound healing response going along with massive proliferation of basal keratinocytes, basically quiescent under non-irradiated, homeostatic conditions...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715113/calcium-deposition-in-photocrosslinked-poly-pro-hyp-gly-hydrogels-encapsulated-rat-bone-marrow-stromal-cells
#7
Farah Nurlidar, Keisuke Yamane, Mime Kobayashi, Kayo Terada, Tsuyoshi Ando, Masao Tanihara
Reproducing the features of the extracellular matrix is important for fabricating three-dimensional (3D) scaffolds for tissue regeneration. A collagen-like polypeptide, poly(Pro-Hyp-Gly), is a promising material for 3D scaffolds because of its excellent physical properties, biocompatibility, and biodegradability. In this paper, we present a novel photocrosslinked poly(Pro-Hyp-Gly) hydrogel as a 3D scaffold for simultaneous rat bone marrow stromal cell (rBMSC) encapsulation. The hydrogels were fabricated using visible-light photocrosslinking at various concentrations of methacrylated poly(Pro-Hyp-Gly) (20-50 mg/mL) and irradiation times (3 or 5 min)...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714574/a-humanised-tissue-engineered-bone-model-allows-species-specific-breast-cancer-related-bone-metastasis-in-vivo
#8
V M C Quent, A V Taubenberger, J C Reichert, L C Martine, J A Clements, D W Hutmacher, D Loessner
Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer-related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the complex cancer cell-bone interactions. In this study, two established tissue-engineered bone constructs (TEBCs) were applied to a breast cancer-related metastasis model. A cylindrical medical-grade polycaprolactone-tricalcium phosphate scaffold produced by fused deposition modelling (scaffold 1) was compared with a tubular calcium phosphate-coated polycaprolactone scaffold fabricated by solution electrospinning (scaffold 2) for their potential to generate ectopic humanised bone in NOD/SCID mice...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714570/human-osteoarthritic-chondrons-outnumber-patient-and-joint-matched-chondrocytes-in-hydrogel-culture-future-application-in-autologous-cell-based-oa-cartilage-repair
#9
Miriam Rothdiener, Tatiana Uynuk-Ool, Norbert Südkamp, Matthias Aurich, Alan J Grodzinsky, Bodo Kurz, Bernd Rolauffs
Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA-chondrons may outperform OA-chondrocytes. We compared patient- and joint surface-matched human OA-chondrons vs. OA-chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, collagen II synthesis (CPII) and degradation (C2C), and sGAG...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714568/exposure-to-reversine-affects-the-chondrocyte-morphology-and-phenotype-in-vitro
#10
S Gasparini, F Villa, L Molfetta, E Repaci, P Castagnola, R Quarto, P Giannoni
Articular chondrocytes derived from osteoarthritic tissues (OA-HAC) show a severely reduced chondrogenic commitment. This impairment undermines their use for tissue-engineered cartilage repair, which relies on cell proliferation and growth to meet the therapeutic needs but also on efficient cell plasticity to recover the chondrogenic phenotype. Reversine (Rev), a 2,6-disubstituted purine inhibitor of spindle-assembly checkpoints, was described to convert differentiated mesenchymal cells to their undifferentiated precursors...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714550/optimal-timing-and-frequency-of-bone-marrow-soup-therapy-for-functional-restoration-of-salivary-gland-injured-by-single-dose-or-fractionated-irradiation
#11
Dongdong Fang, Sixia Shang, Younan Liu, Mohammed Bakkar, Yoshinori Sumita, Jan Seuntjens, Simon D Tran
Injections of bone marrow (BM) cell extract, known as "BM Soup", was previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However the optimal starting time and frequency to maintain BM Soup therapeutic efficacy remains unknown. This study tests the optimal starting time and frequency of BM Soup injections in mice radiated with either a single dose or fractionated dose. First, BM Soup treatment is started at 1, 3, or 7 weeks post-IR, while positive (non-IR) and negative (IR) control mice receive injections of saline (vehicle control)...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714548/role-of-the-protease-corin-in-chondrogenic-differentiation-of-human-bone-marrow-derived-mesenchymal-stem-cells
#12
Haibin Zhou, Jinsong Zhu, Meng Liu, Qingyu Wu, Ningzheng Dong
Mesenchymal stem cells (MSCs) have the potency to differentiate into chondrocytes, osteocytes and adipocytes. Corin is a cardiac protease that activates the natriuretic peptides, thereby regulating blood volume and pressure. In addition to the heart, corin gene up-regulation was reported in bone marrow- and adipose tissue-derived MSCs that underwent osteogenic differentiation. To date, the biological significance of corin expression in MSC differentiation remains unknown. In this study, we isolated and cultured human bone marrow-derived MSCs that were capable of undergoing chondrogenic, osteogenic and adipogenic lineage differentiation...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714276/human-platelet-lysate-supports-the-formation-of-robust-human-periodontal-ligament-cell-sheets
#13
Bei-Min Tian, Rui-Xin Wu, Chun-Sheng Bi, Xiao-Tao He, Yuan Yin, Fa-Ming Chen
The use of stem cell-derived sheets has become increasingly common in a wide variety of biomedical applications. Although substantial evidence has demonstrated that human platelet lysate (PL) can be used for therapeutic cell expansion, either as a substitute for or as a supplement to xenogeneic fetal bovine serum (FBS), its impact on cell sheet production remains largely unexplored. In this study, we manufactured periodontal ligament stem cell (PDLSC) sheets in vitro by incubating PDLSCs in sheet-induction media supplemented with various ratios of PL and FBS, i...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714236/effectiveness-of-tissue-engineered-three-dimensional-bioactive-graft-on-bone-healing-and-regeneration-an-in-vivo-study-with-significant-clinical-value
#14
Mostafa Shahrezaie, Ali Moshiri, Babak Shekarchi, Ahmad Oryan, Nicola Maffulli, Javad Parvizi
Several strategies have been used to promote bone repair; with many failing due to the lack of osteoinduction. We describe our approach for promoting bone healing that attempts to overcome prior shortcomings. First, we compared the role of different concentrations of Gelatin (Gel), nanostructured-hydroxyapatite (nHA), Simvastatin (Sim) and nHA-Sim particles on healing of small femoral bone defects in rabbits. The effective concentration of each of them was studied, and then a three-dimensional porous scaffold was designed using Gel, nHA and Sim which was then cross-linked with Genipin...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714140/prevascularization-of-natural-nanofibrous-extracellular-matrix-for-engineering-completely-biological-3d-prevascularized-tissues-for-diverse-applications
#15
Lijun Zhang, Zichen Qian, Mitchell Tahtinen, Shaohai Qi, Feng Zhao
Self-sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered tissues. As an important mural cell, human mesenchymal stem cells (hMSCs) not only stabilize the engineered microvessel network, but also preserve their multi-potency when grown under optimal culture conditions...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714275/fucoidan-induced-osteogenic-differentiation-promotes-angiogenesis-by-inducing-vascular-endothelial-growth-factor-secretion-and-accelerates-bone-repair
#16
Beom-Su Kim, Sun-Sik Yang, Hyung-Keun You, Hong-In Shin, Jun Lee
Osteogenesis and angiogenesis, including cell-cell communication between blood vessel cells and bone cells, are essential for bone repair. Fucoidan is a chemical compound that has a variety of biological activities. It stimulates osteoblast differentiation in human mesenchymal stem cells (MSCs), which in turn induces angiogenesis. However, the mechanism by which this communication between osteoblasts and endothelial cells is mediated remains unclear. Thus, the aim of this study was to clarify the relationship between fucoidan-induced osteoblastic differentiation in MSCs and angiogenesis in endothelial cells...
July 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28672097/three-dimensional-neural-cultures-produce-networks-that-mimic-native-brain-activity
#17
Justin L Bourke, Anita F Quigley, Serena Duchi, Cathal D O'Connell, Jeremy M Crook, Gordon G Wallace, Mark J Cook, Robert M I Kapsa
Development of brain function is critically dependent on neuronal networks organised through three dimensions. Culture of central nervous system neurons has traditionally been limited to two dimensions, restricting growth patterns and network formation to a single plane. Here, with the use of multichannel extracellular microelectrode arrays, we demonstrate that neurons cultured in a true three-dimensional environment recapitulate native neuronal network formation and produce functional outcomes more akin to in vivo neuronal network activity...
July 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28658717/bone-marrow-on-a-chip-long-term-culture-of-human-hematopoietic-stem-cells-in-a-3d-microfluidic-environment
#18
Stefan Sieber, Lorenz Wirth, Nino Cavak, Marielle Koenigsmark, Uwe Marx, Roland Lauster, Mark Rosowski
Multipotent hematopoietic stem and progenitor cells (HSPCs) are the source for all blood cell types. The bone marrow stem cell niche in which the HSPCs are maintained is known to be vital for their maintenance. Unfortunately, to this date no in vitro model exists that truthfully mimics the aspects of the bone marrow niche and simultaneously allows the long-term culture of HSPCs. In this study, we present a novel 3D co-culture model, based on a hydroxyapatite coated zirconium oxide scaffold, comprising of human mesenchymal stromal cells (MSCs) and cord blood derived HSPCs, enabling successful HSPC culture for a time span of 28 days within the microfluidic Multi-Organ-Chip (MOC)...
June 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28636192/intestinal-barrier-integrity-and-inflammatory-bowel-disease-stem-cell-based-approaches-to-regenerate-the-barrier
#19
REVIEW
Fredrik E O Holmberg, Jannie Pedersen, Peter Jørgensen, Christoffer Soendergaard, Kim B Jensen, Ole H Nielsen
Disruption of normal barrier function is a fundamental factor in the pathogenesis of inflammatory bowel disease, which includes increased epithelial cell death, modified mucus configuration, altered expression and distribution of tight junction-proteins, along with a decreased expression of antimicrobial peptides. Inflammatory bowel disease is associated with life-long morbidity for affected patients, and both the incidence and prevalence is increasing globally, resulting in substantial economic strain for society...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#20
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
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