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Journal of Tissue Engineering and Regenerative Medicine

Yong-Can Huang, Yong Hu, Zhen Li, Keith D K Luk
A biomaterial-based strategy is employed to regenerate the degenerated intervertebral disc, which is considered a major generator of neck and back pain. Although encouraging enhancements in the anatomy and kinematics of the degenerative disc have been gained by biomaterials with various formulations in animals, the number of biomaterials tested in humans is rare. At present, most studies that involve the use of newly-developed biomaterials focus on regeneration of the degenerative disc, but not pain relief...
August 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
Paolo F Sánchez, Eric M Brey, Juan Carlos Briceño
Despite the wide variety of tissue-engineered vascular grafts that are currently being developed, autologous vessels, such as the saphenous vein, are still the gold standard grafts for surgical treatment of vascular disease. Recently developed technologies have shown promising results in preclinical studies, but they still do not overcome the issues that native vessels present, and only a few have made the transition into clinical use. The endothelial lining is a key aspect for the success or failure of the grafts, especially on smaller diameter grafts (<5 mm)...
August 5, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yoshiki Tani, Masato Sato, Munetaka Yokoyama, Miyuki Yokoyama, Takumi Takahashi, Eriko Toyoda, Eri Okada, Shinsei Fujimura, Hideyuki Maruki, Yoshiharu Kato, Masayuki Yamato, Teruo Okano, Joji Mochida
We have reported the usefulness of chondrocyte sheets on articular cartilage repair in animal experiments. Here, we investigated the regenerative effects of EP2 signalling with or without chondrocyte sheets. Forty-five rabbits were used, with six rabbits in each of the six groups and nine rabbits for chondrocytes and synovial cells harvesting to fabricate triple-layered chondrocyte sheets: osteochondral defect only (control, Group A), EP2 agonist (Group B), EP2 antagonist (Group C), chondrocyte sheets (Group D), EP2 agonist and chondrocyte sheets (Group E), and EP2 antagonist and chondrocyte sheets (Group F)...
August 3, 2018: Journal of Tissue Engineering and Regenerative Medicine
Radu A Popescu, Klara Magyari, Marian Taulescu, Adriana Vulpoi, Cristian Berce, Sidonia Bogdan, Cristina Lelescu, Alexandra Dreancă, Oana Tudoran, Ionel Papuc, Lucian Baia
Composites based on sodium alginate, pullulan, and bioactive SiO2 -CaO-P2 O5 glass-ceramics with copper oxide were prepared as capsules. The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy. The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance...
August 1, 2018: Journal of Tissue Engineering and Regenerative Medicine
Felipe Monte, Tugba Cebe, Daniel Ripperger, Fareed Ighani, Hristo V Kojouharov, Benito M Chen, Harry K W Kim, Pranesh B Aswath, Venu G Varanasi
Oxidative stress, induced by harmful levels of reactive oxygen species, is a common occurrence that impairs proper bone defect vascular healing through the impairment of endothelial cell function. Ionic Silicon released from silica-based biomaterials, can upregulate hypoxia-inducible factor-1α(HIF-1α). Yet, it is unclear whether ionic Si can restore endothelial cell function under oxidative stress conditions. Therefore, we hypothesized that ionic silicon can help improve human umbilical vein endothelial cells'(HUVECs) survival under toxic oxidative stress...
July 30, 2018: Journal of Tissue Engineering and Regenerative Medicine
Chih-Hsin Lin, Jimmy Jiun-Ming Su, Shyh-Yuan Lee, Yuan-Min Lin
For stem cell differentiation, the microenvironment can play an important role, and hydrogels can provide a three-dimensional microenvironment to allow native cell growth in vitro. A challenge is that the stem cell's differentiation can be influenced by the matrix stiffness. We demonstrate a low-toxicity method to create different stiffness matrices, by using a photopolymerizable gelatin methacrylate (GelMA) hydrogel cross-linked by blue light (440 nm). The stiffness and porosity of GelMA hydrogel is easily modified by altering its concentration...
July 30, 2018: Journal of Tissue Engineering and Regenerative Medicine
Verónica Álvarez, Francisco Miguel Sánchez-Margallo, Beatriz Macías-García, María Gómez-Serrano, Inmaculada Jorge, Jesús Vázquez, Rebeca Blázquez, Javier G Casado
Endometrial mesenchymal stem cells (endMSCs) reside in the basal and functional layer of human endometrium and participate in tissue remodelling, which is required for maintaining the regenerative capacity of the endometrium. The endMSCs are multipotent stem cells and exhibit immunomodulatory effects. This paper aimed to evaluate the regulatory effects of extracellular vesicles derived from endMSCs (EV-endMSCs) in the setting of T cell activation. In vitro stimulations of lymphocytes were performed in the presence of EV-endMSCs...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
Ramila Joshi, Brendan Fuller, Bobak Mosadegh, Hossein Tavana
Efforts to enhance the efficiency of neural differentiation of stem cells are primarily focused on exogenous modulation of physical niche parameters such as surface topography and extracellular matrix proteins, or addition of certain growth factors or small molecules to culture media. We report a novel neurogenic niche to enhance the neural differentiation of embryonic stem cells (ESCs) without any external intervention by micropatterning ESCs into spatially organized colonies of controlled size and interspacing...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
Masako Fujioka-Kobayashi, Kanji Tsuru, Hirokazu Nagai, Kenji Fujisawa, Takaharu Kudoh, Go Ohe, Kunio Ishikawa, Youji Miyamoto
Carbonate apatite-coated calcium carbonate (CO3 Ap/CaCO3 ) was fabricated through a dissolution-precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3 Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3 , 10% CO3 Ap/CaCO3 , 30% CO3 Ap/CaCO3 , and CO3 Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3 Ap...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
R Nicole Howie, Emily Durham, Brayden Oakes, Zachary Grey, Jason Smith, Phil Campbell, Amanda LaRue, Martin Steed, Robin Muise-Helmericks, James Cray
Many variables serve to alter the process of bone remodelling and diminish regeneration including the size and nature of the wound bed and health status of the individual. To overcome these inhibitory factors, tissue-engineered osteoconductive scaffolds paired with various growth factors have been utilized clinically. However, many limitations still remain, for example, bone morphogenetic protein 2 (BMP2) can lead to rampant inflammation, ectopic bone formation, and graft failure. Here, we studied the ability for a nanofiber scaffold (Talymed) to accelerate BMP2 growth factor-induced bone healing compared with the traditional absorbable collagen sponge (ACS) delivery system...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
Takumi Takahashi, Masato Sato, Eriko Toyoda, Miki Maehara, Daichi Takizawa, Hideyuki Maruki, Ayako Tominaga, Eri Okada, Ken Okazaki, Masahiko Watanabe
Research on cartilage regeneration has developed novel sources for human chondrocytes and new regenerative therapies, but appropriate animal models for translational research are needed. Although rabbit models are frequently used in such studies, the availability of immunocompromised rabbits is limited. Here, we investigated the usefulness of an immunosuppressed rabbit model to evaluate directly the efficacy of human chondrocyte sheets through xenogeneic transplantation. Human chondrocyte sheets were transplanted into knee osteochondral defects in Japanese white rabbits administered with immunosuppressant tacrolimus at a dosage of 0...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
Lisha Zheng, Lingyu Zhang, Luoping Chen, Jingyi Jiang, Xiaocheng Zhou, Ming Wang, Yubo Fan
The dental pulp stem cells (DPSCs) are a population of mesenchymal stem cells, which have multilineage potential and high proliferation. DPSCs are regarded as a promising tool for tissue regeneration of dentine, dental pulp, bone, cartilage, and muscle. Recently, magnetic materials have become commonly applied in dental clinics. Static magnetic field has been reported to regulate the proliferation, migration, or differentiation of stem cells. However, whether static magnetic fields affect DPSCs is still unknown...
July 29, 2018: Journal of Tissue Engineering and Regenerative Medicine
Mary Patrice Eastwood, Willeke F Daamen, Luc Joyeux, Savitree Pranpanus, Rita Rynkevic, Lucie Hympanova, Michiel W Pot, Danique J Hof, Ghislaine Gayan-Ramirez, Toin H van Kuppevelt, Eric Verbeken, Jan Deprest
Gore-Tex® is a widely used durable patch for repair of congenital diaphragmatic defects yet may result in complications. We compared Gore-Tex with a composite of a radial pore-orientated collagen scaffold (RP-Composite) and clinically used porcine small intestinal submucosa (SIS; Surgisis®) in a rabbit model for diaphragmatic hernia. The growing rabbit mimics the rapid rib cage growth and reherniation rates seen in children. We created and immediately repaired left hemidiaphragmatic defects in 6-week-old rabbits with Gore-Tex, SIS, and an RP-Composite scaffold...
July 28, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yanfang Ding, Wei Liu, Weiting Yu, Shenzhou Lu, Ming Liu, David L Kaplan, Xiuli Wang
Multidrug resistance (MDR) is one of the major obstacles to improving outcomes of chemotherapy in tumour patients. However, progress has been slow to overcome this phenomenon due to the limitations of current cell/tissue models in recapitulating MDR behaviour of tumour cells in vitro. To address this issue, a more pathologically relevant, three-dimensional (3D) culture of human breast cancer cells was developed by seeding the adriamycin-resistant cells MCF-7R in silk-collagen scaffolds. The cultures of the parental cell line MCF-7 served as controls...
July 28, 2018: Journal of Tissue Engineering and Regenerative Medicine
Ulf Krister Hofmann, Jessica Steidle, Marina Danalache, Florian Bonnaire, Christian Walter, Bernd Rolauffs
A type VI collagen-rich pericellular matrix (PCM) encloses both intervertebral disk (IVD) and articular cartilage chondrocytes. In the latter, the PCM protects the chondrocytes from mechanical overload, whereas tissue degeneration is associated with PCM destruction. As little is known about the IVD PCM, we investigated chondrocyte survival after mechanical overload as well as PCM structural integrity as a function of clinical tissue degeneration. The hypothesis was that IVD degeneration may affect PCM integrity and overload-related chondrocyte survival...
July 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
Luke G Major, Yu Suk Choi
The popular reductionist approach towards stem cell differentiation has identified an array of factors that can induce lineage-specific differentiation. Whether these variables direct differentiation in a multivariable setting in the same way as a single-variable setting is a question of interest. Polyacrylamide hydrogels of specific stiffness were microcontact printed with fibronectin in specific shapes and sizes to construct 54 different extracellular matrix types of defined stiffness, shape, and area. Three media types were also used to investigate the effect of both biomechanical and biochemical inducers of differentiation on adipose-derived stem cells...
July 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
Majid Ebrahimi, Serge Ostrovidov, Sahar Salehi, Sang Bok Kim, Hojae Bae, Ali Khademhosseini
Bioengineered functional muscle tissues are beneficial for regenerative medicine due to their treatment potential for various debilitating disorders, including myopathy and traumatic injuries. However, the contractile properties of engineered muscle constructs are lacking compared to their native counterparts. Here, we used microfluidic spinning to fabricate photocrosslinkable gelatin methacryloyl (GelMA) hydrogel fibers with well-defined surface morphologies for engineering muscle tissues. We examined whether the combination of topographical cues from surface micropatterning and biochemical stimulation with recombinant agrin can improve the generation of bioengineered muscle tissue...
July 26, 2018: Journal of Tissue Engineering and Regenerative Medicine
Mireille M J P E Sthijns, Clemens A van Blitterswijk, Vanessa L S LaPointe
One of the biggest challenges in tissue engineering and regenerative medicine is to incorporate a functioning vasculature to overcome the consequences of a lack of oxygen and nutrients in the tissue construct. Otherwise, decreased oxygen tension leads to incomplete metabolism and the formation of the so-called reactive oxygen species (ROS). Cells have many endogenous antioxidant systems to ensure a balance between ROS and antioxidants, but if this balance is disrupted by factors such as high levels of ROS due to long-term hypoxia, there will be tissue damage and dysfunction...
July 25, 2018: Journal of Tissue Engineering and Regenerative Medicine
Barbara Dozza, Francesca Salamanna, Massimiliano Baleani, Gianluca Giavaresi, Annapaola Parrilli, Lorenzo Zani, Enrico Lucarelli, Lucia Martini, Milena Fini, Davide Maria Donati
Nonunion treatment has a high rate of success, although recalcitrant nonunion may determine the need for amputation. Therefore, new treatment options are continuously investigated in order to further reduce the risk of nonunion recurrence. This study aimed to (a) develop a new large animal model for bone atrophic nonunion and (b) compare the efficacy of demineralized bone matrix (DBM) and DBM in combination with mesenchymal stem cells (MSC) in the new nonunion model. The new model consists of a noncritical, full-thickness segmental defect created in the sheep tibia, stabilized by an intramedullary nail, and involves the creation of a locally impaired blood supply achieved through periosteum excision and electrocauterization of the stump ends...
July 25, 2018: Journal of Tissue Engineering and Regenerative Medicine
Arne Hendrik Boecker, Ahmet Bozkurt, Bong Sung Kim, Haktan Altinova, Julian Tank, Ronald Deumens, Rene Tolba, Joachim Weis, Gary Anthony Brook, Norbert Pallua, Sabien Geraldine Antonia van Neerven
The reconstruction of peripheral nerve injuries is clinically challenging, and today, the autologous nerve transplantation is still considered as the only gold standard remedy for nerve lesions where a direct nerve coaptation is not possible. Nevertheless, the functional merits of many biomaterials have been tested as potential substitutes for the autologous nerve transplant. One of the strategies that have been pursued is the combination of bioengineered nerve guides with cellular enrichment. In this present study, we combined the previously evaluated collagen-based and microstructured nerve guide Perimaix with olfactory ensheathing cell enrichment...
July 25, 2018: Journal of Tissue Engineering and Regenerative Medicine
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