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Journal of Tissue Engineering and Regenerative Medicine

Rosalyn D Abbott, Francis E Borowsky, Carlo A Alonzo, Adam Zieba, Irene Georgakoudi, David L Kaplan
Obesity is a risk factor for a myriad of diseases including diabetes, cardiovascular dysfunction, cirrhosis and cancer and there is a need for new systems to study how excess adipose tissue relates to the onset of disease processes. This study provides proof of concept patient-specific tissue models of human white adipose tissue to accommodate the variability in human samples. Our 3D tissue engineering approach established lipolytic responses and changes in insulin-stimulated glucose uptake from small volumes of human lipoaspirate, making this methodology useful for patient specific sample source assessments of treatment strategies, drug responses, disease mechanisms and other responses that vary between patients...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
Adrien Moya, Nathanaël Larochette, Marianne Bourguignon, Hanane El-Hafci, Esther Potier, Hervé Petite, Delphine Logeart-Avramoglou
In the present study, we evaluated the benefits of an adipogenic predifferentiation, the pathway most closely related to osteoblastogenesis, on the pro-osteogenic potential of human adult multipotent bone marrow stromal cells (hBMSCs), both in vitro and in vivo. Adipogenic differentiation of hBMSCs for 14 days resulted in a heterogeneous cell population from which the most adipogenic-committed cells were eliminated by their lack of re-adhesion ability. Our results provided evidence that the select adherent adipogenic differentiated hBMSCs (sAD+ cells) express a gene profile characteristic of both adipogenic and osteogenic lineages...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
Mathieu Maisani, Sophia Ziane, Camille Ehret, Lucie Levesque, Robin Siadous, Jean-François Le Meins, Pascale Chevalier, Philippe Barthélémy, Hugo De Oliveira, Joëlle Amédée, Diego Mantovani, Olivier Chassande
Tissue engineering is a promising alternative to autografts, allografts or biomaterials to address the treatment of severe and large bone lesions. Classically, tissue engineering products associate a scaffold and cells, and are implanted or injected into the lesion. These cells must be embedded in an appropriate biocompatible scaffold, which offers a favourable environment for their survival and differentiation. Here, we designed a composite hydrogel composed of collagen I, an extracellular matrix protein widely used in several therapeutic applications, which we associated with a physical hydrogel generated from a synthetic small amphiphilic molecule...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
Yoshiya Asano, Hiroshi Shimoda, Michiya Matsusaki, Mitsuru Akashi
Transplantation of engineered tissues with microvascular structure is advancing towards therapeutic application to improve the flow of blood and/or lymphatic fluids. In lymphatic disorders, transplantation of tissue-engineered lymphatic grafts can be an ideal treatment for draining excessive lymphatic fluid. In this study, we examined the transplantation of three-dimensional artificial human lymphatic network tissue (AHLT) fabricated by the cell accumulation technique into the subcutaneous tissue and fascia of mice...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
Jeremy A Schaefer, Pilar A Guzman, Sonja B Riemenschneider, Timothy J Kamp, Robert T Tranquillo
Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack pre-formed microvascularization and are therefore limited in thickness and force production. In the present study, we sought to assess whether a bi-layer patch composed of a layer made from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and a microvessel layer composed of self-assembled human blood outgrowth endothelial cells (BOECs) and pericytes (PCs) was capable of engrafting on the epicardial surface of a nude rat infarct model and becoming perfused by the host four weeks after acute implantation...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
M-A Malahias, V S Nikolaou, E O Johnson, M-K Kaseta, S-T Kazas, G C Babis
PURPOSE: To demonstrate whether and to what extent a single injection of Platelet-Rich Plasma (PRP), under ultrasound guidance, can improve the clinical symptoms of patients with a mild to moderate carpal tunnel syndrome (CTS). METHODS: We conducted a prospective, randomized, clinical study including 50 patients suffering from mild to moderate CTS for a minimum duration of 3-months. Patients were randomly divided into two groups: A (26 patients) received an ultrasound-guided PRP injection into the carpal tunnel, whereas Group B (24 patients) was injected with placebo (0...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
Ram-Kumar Ramani-Mohan, Ivo Schwedhelm, Anna Finne-Wistrand, Melanie Krug, Thomas Schwarz, Franz Jakob, Heike Walles, Jan Hansmann
Mesenchymal stem cells play a major role during bone remodeling and are thus of high interest for tissue engineering and regenerative medicine applications. Mechanical stimuli, i.e. deformation strain and interstitial fluid-flow-induced shear stress, promote osteogenic lineage commitment. However, the predominant physical stimulus that drives early osteogenic cell maturation is not clearly identified. The evaluation of each stimulus is challenging, as deformation and fluid-flow-induced shear stress interdepend...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
Wenxia Yan, Hanping Liu, Xiaoyuan Deng, Ying Jin, Ning Wang, Jing Chu
The regional injection of connective tissue growth factor (CTGF) for diabetic wound healing requires multiple components and results in a substantial loss of its biological activity. Acellular dermal matrix (ADM) scaffolds are optimal candidates for delivering these factors to local ischemic environments. In this study, we explored whether CTGF loaded on ADM scaffolds can enhance fibronectin (FN) expression to accelerate diabetic wound healing via the Protein kinase C (PKC) signaling pathway. The performance of CTGF and CTGF+PKC inhibitor, which were loaded on ADM scaffolds to treat dorsal skin wounds in streptozotocin (STZ)-induced diabetic mice, were evaluated with naked ADM as a control...
September 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
Babitha Sekar, Meenakshi Annamalai, Michal Marcin Dykas, Surajit Saha, Kingshuk Poddar, Jayarama Reddy Venugopal, Seeram Ramakrishna, Thirumalai Venkatesan, Purna Sai Korrapati
A biomimetic Zein polydopamine (PDA) based nanofiber scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide (TiO2 ) nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to TiO2 nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nano topographical stimulation of electrospun Zein PDA nanofibers were examined for its enhanced osteogenic expression of human fetal osteoblast (hFOB) cells...
September 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
Arturo Ibáñez-Fonseca, Teresa L Ramos, Israel González de Torre, Luis Ignacio Sánchez-Abarca, Sandra Muntión, Francisco Javier Arias, María Consuelo Del Cañizo, Matilde Alonso, Fermín Sánchez-Guijo, José Carlos Rodríguez-Cabello
Biocompatibility studies, especially innate immunity induction, in vitro and in vivo cytotoxicity, and fibrosis, are often lacking for many novel biomaterials including recombinant protein-based ones, such as elastin-like recombinamers (ELRs), and has not been extensively explored in the scientific literature, in contrast to traditional biomaterials. Herein we present the results from a set of experiments designed to elucidate the preliminary biocompatibility of two types of ELRs that are able to form extracellular matrix-like hydrogels through either physical or chemical crosslinking, both of which are intended for different applications in tissue engineering and regenerative medicine (TERM)...
September 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
Hugo R Caires, Patrícia Barros da Silva, Mário A Barbosa, Catarina R Almeida
The biological response to implanted biomaterials is a complex and highly coordinated phenomenon involving many different cell types that interact within 3D microenvironments. Here, we increased the complexity of a 3D platform to include at least three cell types that play a role in the host response upon scaffold implantation. With this system it was possible to address how immune responses triggered by 3D biomaterials mediate recruitment of stromal cells that promote tissue regeneration, Mesenchymal Stromal/Stem Cells (MSC), or a foreign body response, fibroblasts...
September 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
Mira Park, Hyung Chul Kim, Okjoon Kim, Helen Lew
Different damage factors are known to trigger cell death in the optic nerves. Use of mesenchymal stem cells is a possible treatments option for traumatic optic nerve injury due to their ability to secrete protective cytokines and recovery factors. In this study, we investigated the neuroprotective effects of human placenta derived mesenchymal stem cells (hPMSCs) using an established optic nerve compression model, and model of R28 cells that were exposed to hypoxia. 40% of axon death was seen in induced in vivo optic nerve injury model (p<0...
August 31, 2017: Journal of Tissue Engineering and Regenerative Medicine
Pedro S Babo, Xinjie Cai, Adelina S Plachokova, Rui L Reis, John Jansen, Manuela E Gomes, X Frank Walboomers
With currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved. In this study, a combined compartmentalized system was tested, composed of (1) a platelet lysate (PL)-based construct, which was placed along the root aiming to regenerate the root cementum and periodontal ligament, and (2) a calcium phosphate cement composite incorporated with hyaluronic acid microspheres loaded with PL, aiming to promote the regeneration of alveolar bone. This bi-layered system was assessed in a 3-wall periodontal defect in Wistar rats...
August 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
Doo Yeon Kwon, Ji Hoon Park, So Hee Jang, Joon Yeong Park, Ju Woong Jang, Byoung Hyun Min, Wan-Doo Kim, Hai Bang Lee, Junhee Lee, Moon Suk Kim
Recently, computer-designed three-dimensional (3D) printing techniques have emerged as an active research area with almost unlimited possibilities. In this study, we used a computer-designed 3D scaffold to drive new bone formation in a bone defect. Poly-L-lactide (PLLA) and bioactive β-tricalcium phosphate (TCP) were simply mixed to prepare ink. PLLA+TCP showed good printability from the micronozzle and solidification within few seconds, indicating that it was indeed printable ink for layer-by-layer printing...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
Jia Xu, Yuxin Sun, Tianyi Wu, Yang Liu, Liu Shi, Jinfang Zhang, Qinglin Kang, Yimin Chai, Gang Li
Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). The present study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwent right tibial transverse osteotomy, and were randomly divided into four groups after lengthening: control (no manipulation) and three experimental groups assigned based on the period of accordion maneuvers in the consolidation phase (Groups 1, 2, and 3 with accordion technique applied at weeks 1, 3, and 5, respectively)...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
Jovana Zvicer, Bojana Obradovic
Intervertebral discs are normally exposed to variety of loads and stresses but hydrostatic pressure (HP) could be the main biosignal for chondrogenic cell differentiation and maintenance of this tissue. Although there are simple approaches to intermittently expose cell cultures to HP in separate material testing devices, utilization of biomimetic bioreactors aiming to provide in vitro conditions mimicking those found in vivo, attracts special attention. However, design of such bioreactors is complex due to the requirement of high HP magnitudes (up to 3 MPa) applied in different regimes mimicking pressures arising in IVD during normal daily activities...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
Bradley W Tuft, Linjing Xu, Braden Leigh, Daniel Lee, C Allan Guymon, Marlan R Hansen
Developing and regenerating neurites respond to a variety of biophysical and biochemical cues in their microenvironment to reach target cells and establish appropriate synapses. Defining the hierarchal relationship of both types of cues to direct neurite growth carries broad significance for neural development, regeneration, and, in particular, engineering of neural prostheses that improve tissue integration with native neural networks. In this work, chemorepulsive biochemical borders are established on substrates with a range of surface microfeatures to determine the potential of physical cues to overcome conflicting biochemical cues...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
Shiuhyang Kuo, Hyungjin Myra Kim, Zhifa Wang, Eve L Bingham, Atsuko Miyazawa, Cynthia L Marcelo, Stephen E Feinberg
Immunologically inert allogeneic acellular dermal scaffolds provide a matrix with molecular architecture close to native tissues, which synthetic scaffolds cannot. Not all nature-derived scaffolds possess the same biological and physical properties. The different properties of scaffolds supporting cellular growth used for manufacturing tissue engineered grafts could lead to different implantation results. The scaffold properties should be carefully considered in order to meet the expected outcomes of tissue engineered grafts...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
Stephan Detert, Christof Stamm, Christien Beez, Falk Diedrichs, Jochen Ringe, Sophie Van Linthout, Martina Seifert, Carsten Tschöpe, Michael Sittinger, Marion Haag
Cardiac-derived adherent proliferating (CardAP) cells obtained from endomyocardial biopsies (EMBs) with known anti-fibrotic and pro-angiogenic properties are good candidates for the autologous therapy of end-stage cardiac diseases such as dilated cardiomyopathy. However, due to the limited number of CardAP cells that can be obtained from EMBs, our aim is to isolate cells with similar properties from other regions of the heart with comparable tissue architecture. Here, we introduce the atrial appendage as a candidate region...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
Madiha Khurshid, Aillette Mulet-Sierra, Adetola Adesida, Arindom Sen
Osteoarthritis (OA) is a painful disease, characterized by progressive surface erosion of articular cartilage. The use of human articular chondrocytes (hACs) sourced from OA patients has been proposed as a potential therapy for cartilage repair, but this approach is limited by the lack of scalable methods to produce clinically relevant quantities of cartilage-generating cells. Previous studies in static culture have shown that hACs co-cultured with human mesenchymal stem cells (hMSCs) as 3D pellets can upregulate proliferation and generate neocartilage with enhanced functional matrix formation relative to that produced from either cell type alone...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
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