journal
MENU ▼
Read by QxMD icon Read
search

Journal of Tissue Engineering and Regenerative Medicine

journal
https://www.readbyqxmd.com/read/28636192/intestinal-barrier-integrity-and-inflammatory-bowel-disease-stem-cell-based-approaches-to-regenerate-the-barrier
#1
REVIEW
Fredrik E O Holmberg, Jannie Pedersen, Peter Jørgensen, Christoffer Soendergaard, Kim B Jensen, Ole H Nielsen
Disruption of normal barrier function is a fundamental factor in the pathogenesis of inflammatory bowel disease, which includes increased epithelial cell death, modified mucus configuration, altered expression and distribution of tight junction-proteins, along with a decreased expression of antimicrobial peptides. Inflammatory bowel disease is associated with life-long morbidity for affected patients, and both the incidence and prevalence is increasing globally, resulting in substantial economic strain for society...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#2
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28633197/influence-of-electron-beam-irradiation-doses-on-bone-regeneration-by-octacalcium-phosphate-collagen-composites
#3
Fumihiko Kajii, Atsushi Iwai, Hidenori Tanaka, Keiko Matsui, Tadashi Kawai, Shinji Kamakura
An octacalcium phosphate and collagen composite (OCP/Col) achieved efficient bone regeneration with excellent resorbability. After the confirmation of its safety and efficacy in preclinical animal studies, the present study investigated the influence of electron beam irradiation doses on bone regeneration by OCP/Col in order to secure its commercialization. OCP/Col was prepared as previously described, and the packed OCP/Col was sterilized using different doses of electron beam irradiation. A standardized defect, 9 mm in diameter, was made in the rat calvarium...
June 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28627746/hepatic-perivascular-mesenchymal-stem-cells-with-myogenic-properties
#4
Sudheer Shenoy P, Bipasha Bose
Pericytes are multipotent mesenchymal stem cells (MSCs) located on the walls of blood vessels in various organs and are characterized as CD146(+) cells.. In this study, we have first immunohistochemically detected such pericytes in the perivascular regions of liver from two mouse genotypes namely wild-type (WT) and myostatin null (Mstn(-/-) ). We further isolated such pericytes using sorting as CD146(+) CD34(-) CD56(-) CD45(-) cells. The main finding of this study involve the contrasting -fibrogenic versus myogenic behavior of liver pericytes from WTMstn(-/-) ) mouse respectively...
June 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28622706/electrical-stimulation-of-microengineered-skeletal-muscle-tissue-effect-of-stimulus-parameters-on-myotube-contractility-and-maturation
#5
Ramin Banan Sadeghian, Majid Ebrahimi, Sahar Salehi
Skeletal muscle tissues engineered in vitro are aneural, short in number of fibers required to function properly, and degenerate rapidly. Electrical stimulation has been widely used to compensate for such lack of neural activity, yet the relationship between the stimulation parameters and the tissue response is subject of debate. Here we study the effect of overnight electrical stimulation (training) on the contractility and maturity of aligned C2C12 myotubes developed on micropatterned gelatin methacryloyl (GelMA) substrates...
June 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28621502/amniotic-membrane-stimulates-cell-migration-by-modulating-transforming-growth-factor-%C3%AE-signaling
#6
Catalina Ruiz-Cañada, Ángel Bernabé-García, Sergio Liarte, Carmen Luisa Insausti, Diego Angosto, José María Moraleda, Gregorio Castellanos, Francisco José Nicolás
Keratinocyte migration is a mandatory aspect of wound-healing. We have previously shown that Amniotic Membrane (AM) applied to chronic wounds assists healing through a process resulting in overexpression of c-Jun at the wound's leading edge. Also, we have demonstrated that amniotic membrane modifies the genetic program induced by TGF-ß in chronic wounds. In this paper, we used a scratch assay of Mv1Lu and HaCaT cells to examine the influence of AM application on the underlying signaling during scratch closure...
June 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28603948/limb-derived-cells-as-a-paradigm-for-engineering-self-assembling-skeletal-tissues
#7
Warnakulasuriya A Fernando, Ioannis Papantoniou, Luis F Mendes, Gabriella Nilsson Hall, Kathleen Bosmans, Wai L Tam, Liliana M Teixeira, Malcolm Moos, Liesbet Geris, Frank P Luyten
Mimicking developmental events has been proposed as a strategy to engineer tissue constructs for regenerative medicine. However, this approach has not yet been investigated for skeletal tissues. Here we demonstrate that ectopic implantation of mouse embryonic day 14.5 long bone anlagen, dissociated into single cells and randomly incorporated in a bioengineered construct, give rise to epiphyseal growth plate like structures, bone, and marrow, which share many morphological and molecular similarities to epiphyseal units that form after transplanting intact long bone anlage, demonstrating substantial robustness and autonomy of complex tissue self-assembly and the overall organogenesis process...
June 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28603879/in-vitro-design-of-mesenchymal-to-epithelial-transition-of-prostate-cancer-metastasis-using-3d-nanoclay-bone-mimetic-scaffolds
#8
M D Shahjahan Molla, Dinesh R Katti, Kalpana S Katti
No abstract text is available yet for this article.
June 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28600846/ectopic-implantation-of-juvenile-osteochondral-tissues-recapitulates-endochondral-ossification
#9
Johnathan Ng, Yiyong Wei, Bin Zhou, Sarindr Bhumiratana, Aonnicha Burapachaisri, Edward Guo, Gordana Vunjak-Novakovic
Subcutaneous implantation in a mouse can be used to investigate tissue maturation in vivo. Here, we demonstrate that this simple model can recapitulate endochondral ossification associated with native skeletal development. By histological and μCT analysis, we investigated morphological changes of immature bovine osteochondral tissues over the course of subcutaneous implantation in immunocompromised mice for up to 10 weeks. We observed multiple similarities between the ectopic process and native endochondral ossification: (i) permanent cartilage retention in the upper zones, (ii) progressive loss of transient cartilage accompanied by bone formation at the interface, and (iii) remodeling of nascent endochondral bone into mature cancellous bone...
June 10, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28600807/multi-layered-silk-film-co-culture-system-for-human-corneal-epithelial-and-stromal-stem-cells
#10
Emily A Gosselin, Tess Torregrosa, Chiara E Ghezzi, Alexandra C Mendelsohn, Rachel Gomes, James L Funderburgh, David L Kaplan
With insufficient options to meet the clinical demand for cornea transplants, one emerging area of emphasis is on cornea tissue engineering. In the present study, the goal was to combine the corneal stroma and epithelium into one co-culture system, in order to monitor both human corneal stromal stem cell (hCSSC) and human corneal epithelial cell (hCE) growth and differentiation into keratocytes and differentiated epithelium in these 3D tissue systems in vitro. Co-culture conditions were first optimized, including the medium, air/liquid interface culture, and surface topography and chemistry of biomaterial scaffold films based on silk protein...
June 10, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28599103/the-use-of-mechano-growth-factor-to-prevent-cartilage-degeneration-in-knee-osteoarthritis
#11
Yang Song, Kang Xu, Can Yu, Lili Dong, Peixing Chen, Yonggang Lv, Martin Y M Chiang, Linhao Li, Wanqian Liu, Li Yang
Previous studies have attached more importance to growth factors in treating cartilage degeneration and osteoarthritis (OA). Here, the capability of mechano growth factor-C24E (MGF) to prevent osteoarthritic cartilage degeneration was evaluated in vitro and in vivo. Using in vitro cultured human OA chondrocytes treated with 10-60 ng/ml MGF for 12 h, we detected the cell proliferation, migration and anabolism of OA chondrocytes. The unfolded protein response (UPR) and the proteins characteristic of OA pathology, such as TGF-β, Smad3 and HIF-2α of OA chondrocytes, were also detected by western blotting...
June 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28599089/initial-graft-size-and-not-the-innate-immune-response-limit-survival-of-engrafted-neural-stem-cells
#12
Stefanie Vogel, Markus Aswendt, Melanie Nelles, Nadine Henn, Gabriele Schneider, Mathias Hoehn
Transplantation of neural stem cells (NSCs) appears as the most promising regenerative therapy for a variety of neurological disorders. Nevertheless, NSC engraftment is limited by the number of surviving cells. To maximize stem cell-mediated effects, timing of implantation and cell number have to be precisely evaluated. Here, we optimized a transgenic murine NSC line for high expression levels of the imaging reporters Luc2 and copGFP. NSCs of 150 000, 75 000, 15 000, or 1 500 cells or Hanks buffered salt solution were implanted into the striatum of nude mice...
June 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28593731/multimodal-3d-imaging-based-on-%C3%AE-mri-and-%C3%AE-ct-techniques-bridges-the-gap-with-histology-in-visualization-of-the-bone-regeneration-process
#13
R Sinibaldi, A Conti, B Sinjari, S Spadone, R Pecci, M Palombo, V S Komlev, M G Ortore, G Tromba, S Capuani, F De Luca, S Caputi, T Traini, S Della Penna
Bone repair/regeneration is usually investigated through x-ray computed microtomography (μCT) supported by histology of extracted samples, to analyze biomaterial structure and new bone formation processes. Magnetic Resonance Imaging (μMRI) shows a richer tissue contrast than μCT, despite at lower resolution, and could be combined with μCT in the perspective of conducting non-destructive 3D investigations of bone. A pipeline designed to combine μMRI and μCT images of bone samples is here described and applied on samples of extracted human jawbone core following bone graft...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28593712/human-adipose-tissue-derived-tenomodulin-positive-subpopulation-of-stem-cells-a-promising-source-of-tendon-progenitor-cells
#14
A I Gonçalves, P M Gershovich, M T Rodrigues, R L Reis, M E Gomes
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human adult multipotent adipose-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of adipose tissue originate highly heterogeneous cell populations that hinder their use in specific tissue-oriented applications. In this study, distinct subpopulations of hASCs were immunomagnetic separated and their tenogenic differentiation capacity evaluated in the presence of several growth factors (GFs), namely EGF, bFGF, TGF-β1 and PDGF-BB, that are well-known regulators of tendon development, growth and healing...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28593699/angiogenic-potential-of-human-mesenchymal-stromal-cell-and-circulating-mononuclear-cell-co-cultures-is-reflected-in-the-expression-profiles-of-proangiogenic-factors-leading-to-endothelial-cell-and-pericyte-differentiation
#15
Katriina Joensuu, Liina Uusitalo-Kylmälä, Teuvo A Hentunen, Terhi J Heino
Endothelial progenitors found among the peripheral blood (PB) mononuclear cells (MNCs) are interesting cells for their angiogenic properties. Mesenchymal stromal cells (MSCs) in turn can produce proangiogenic factors as well as differentiate into mural pericytes, making MSCs and MNCs an attractive co-culture setup for regenerative medicine. In this study, we co-cultured human bone marrow-derived MSCs and PB-derived MNCs in basal or osteoblastic medium without exogenously supplied growth factors to demonstrate endothelial cell, pericyte and osteoblastic differentiation...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28581212/glial-cell-derived-neurotrophic-factor-promotes-dental-pulp-stem-cell-migration
#16
Nan Xiao, Wei Ye Yu, Dawei Liu
Preserving the vitality of the teeth is critical in maintaining the function and esthetics of teeth during dental treatment. Dental pulp stem cells (DPSCs) are mesenchymal cells that are demonstrated to possess stem cell properties, such as self-renewal, proliferation and pluripotency. DPSCs can be obtained through non-invasive procedure from the dental pulp and become potential resources for tissue regeneration. Neurotrophic factors are known to promote survival and growth of neurons. In the present study, we examined the expression of the glial cell derived neurotrophic factor (GDNF) family ligands and receptors, and characterized the intracellular localization of them in DPSCs...
June 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28569438/enzymatic-urease-mediated-mineralization-of-gellan-gum-hydrogel-with-calcium-carbonate-magnesium-enriched-calcium-carbonate-and-magnesium-carbonate-for-bone-regeneration-applications
#17
Timothy E L Douglas, Agata Łapa, Sangram Keshari Samal, Heidi A Declercq, David Schaubroeck, Ana C Mendes, Pascal Van der Voort, Agnieszka Dokupil, Agnieszka Plis, Karel De Schamphelaere, Ioannis S Chronakis, Elżbieta Pamuła, Andre G Skirtach
Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration. Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention. Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated...
June 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28568605/vascular-beds-maintain-pancreatic-tumor-explants-for-ex-vivo-drug-screening
#18
Despina Bazou, Nir Maimon, Gabriel Gruionu, Jelena Grahovac, Giorgio Seano, Hao Liu, Conor L Evans, Lance L Munn
Our understanding of cancer progression or response to therapies would benefit from benchtop, tissue-level assays that preserve the biology and anatomy of human tumors ex vivo. We present methodology for maintaining patient tumor samples ex vivo for the purpose of drug testing in the clinical setting. The harvested tumor biopsy, excised from mice or patients, is integrated into a support tissue that includes stroma and vasculature. This support tissue preserves tumor histo-architecture and relevant expression profiles, and tumor tissues cultured using this system display different sensitivities to chemotherapeutics compared to tumor explants with no supporting tissue...
June 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28568973/substance-p-dexamethasone-encapsulated-plga-scaffold-fabricated-using-supercritical-fluid-process-for-calvarial-bone-regeneration
#19
Su Hee Kim, Ji Eun Kim, Soo Hyun Kim, Youngmee Jung
Poly(lactic-co-glycolic acid) (PLGA) scaffolds encapsulated with substance P (SP) and dexamethasone (Dex) by the supercritical CO2 foaming method were fabricated to treat calvarial bone. We compared the release profiles of SP and Dex according to the incorporation methods using encapsulation or dipping. Ninety percent of the SP or Dex molecules in the scaffolds prepared by the encapsulating method were released by day 14 or day 6, respectively. In vivo real-time assays for human mesenchymal stem cell (hMSC) tracking were performed to confirm the MSC recruitment abilities of the scaffolds...
May 31, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28556569/matrilin-3-co-delivery-with-adipose-derived-mesenchymal-stem-cells-promotes-articular-cartilage-regeneration-in-a-rat-osteochondral-defect-model
#20
Manjunatha S Muttigi, ByoungJu Kim, Bogyu Choi, Arai Yoshie, Hemant Kumar, Inbo Han, Hansoo Park, Soo-Hong Lee
Matrilin-3 is an essential extracellular matrix (ECM) component present only in cartilaginous tissues. Matrilin-3 exerts chondro-protective effects by regulating an anti-inflammatory function and ECM components. We hypothesized that the co-delivery of matrilin-3 with infrapatellar adipose tissue-derived mesenchymal stem cells (Ad-MSCs) may enhance articular cartilage regeneration. Matrilin-3 treatment of Ad-MSCs in serum-free media induced collagen II and aggrecan expression, andmatrilin-3 in chondrogenic media (CM) also enhanced in vitro chondrogenic differentiation...
May 29, 2017: Journal of Tissue Engineering and Regenerative Medicine
journal
journal
41661
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"