journal
MENU ▼
Read by QxMD icon Read
search

Journal of Tissue Engineering and Regenerative Medicine

journal
https://www.readbyqxmd.com/read/28763610/bone-regeneration-by-means-of-a-three-dimensional-printed-scaffold-in-a-rat-cranial-defect
#1
Doo Yeon Kwon, Ji Hoon Park, So Hee Jang, Joon Yeong Park, Ju Woong Jang, Byoung Hyun Min, Wan-Doo Kim, Hai Bang Lee, Junhee Lee, Moon Suk Kim
Recently, computer-designed three-dimensional (3D) printing techniques have emerged as an active research area with almost unlimited possibilities. In this study, we used a computer-designed 3D scaffold to drive new bone formation in a bone defect. Poly-L-lactide (PLLA) and bioactive β-tricalcium phosphate (TCP) were simply mixed to prepare ink. PLLA+TCP showed good printability from the micronozzle and solidification within few seconds, indicating that it was indeed printable ink for layer-by-layer printing...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28763580/enhancement-of-bone-regeneration-with-the-accordion-technique-via-hif-1%C3%AE-vegf-activation-in-a-rat-distraction-osteogenesis-model
#2
Jia Xu, Yuxin Sun, Tianyi Wu, Yang Liu, Liu Shi, Jinfang Zhang, Qinglin Kang, Yimin Chai, Gang Li
Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). The present study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwent right tibial transverse osteotomy, and were randomly divided into four groups after lengthening: control (no manipulation) and three experimental groups assigned based on the period of accordion maneuvers in the consolidation phase (Groups 1, 2, and 3 with accordion technique applied at weeks 1, 3, and 5, respectively)...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28763577/bioreactors-with-hydrostatic-pressures-imitating-physiological-environments-in-intervertebral-discs
#3
REVIEW
Jovana Zvicer, Bojana Obradovic
Intervertebral discs are normally exposed to variety of loads and stresses but hydrostatic pressure (HP) could be the main biosignal for chondrogenic cell differentiation and maintenance of this tissue. Although there are simple approaches to intermittently expose cell cultures to HP in separate material testing devices, utilization of biomimetic bioreactors aiming to provide in vitro conditions mimicking those found in vivo, attracts special attention. However, design of such bioreactors is complex due to the requirement of high HP magnitudes (up to 3 MPa) applied in different regimes mimicking pressures arising in IVD during normal daily activities...
August 1, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28753740/photopolymerized-micropatterns-with-high-feature-frequencies-overcome-chemorepulsive-borders-to-direct-neurite-growth
#4
Bradley W Tuft, Linjing Xu, Braden Leigh, Daniel Lee, C Allan Guymon, Marlan R Hansen
Developing and regenerating neurites respond to a variety of biophysical and biochemical cues in their microenvironment to reach target cells and establish appropriate synapses. Defining the hierarchal relationship of both types of cues to direct neurite growth carries broad significance for neural development, regeneration, and, in particular, engineering of neural prostheses that improve tissue integration with native neural networks. In this work, chemorepulsive biochemical borders are established on substrates with a range of surface microfeatures to determine the potential of physical cues to overcome conflicting biochemical cues...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28752668/comparison-of-two-decellularized-dermal-equivalents
#5
Shiuhyang Kuo, Hyungjin Myra Kim, Zhifa Wang, Eve L Bingham, Atsuko Miyazawa, Cynthia L Marcelo, Stephen E Feinberg
Immunologically inert allogeneic acellular dermal scaffolds provide a matrix with molecular architecture close to native tissues, which synthetic scaffolds cannot. Not all nature-derived scaffolds possess the same biological and physical properties. The different properties of scaffolds supporting cellular growth used for manufacturing tissue engineered grafts could lead to different implantation results. The scaffold properties should be carefully considered in order to meet the expected outcomes of tissue engineered grafts...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28752609/the-atrial-appendage-as-a-suitable-source-to-generate-cardiac-derived-adherent-proliferating-cells-for-regenerative-cell-based-therapies
#6
Stephan Detert, Christof Stamm, Christien Beez, Falk Diedrichs, Jochen Ringe, Sophie Van Linthout, Martina Seifert, Carsten Tschöpe, Michael Sittinger, Marion Haag
Cardiac-derived adherent proliferating (CardAP) cells obtained from endomyocardial biopsies (EMBs) with known anti-fibrotic and pro-angiogenic properties are good candidates for the autologous therapy of end-stage cardiac diseases such as dilated cardiomyopathy. However, due to the limited number of CardAP cells that can be obtained from EMBs, our aim is to isolate cells with similar properties from other regions of the heart with comparable tissue architecture. Here, we introduce the atrial appendage as a candidate region...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28752579/osteoarthritic-human-chondrocytes-proliferate-in-3d-co-culture-with-mesenchymal-stem-cells-in-suspension-bioreactors
#7
Madiha Khurshid, Aillette Mulet-Sierra, Adetola Adesida, Arindom Sen
Osteoarthritis (OA) is a painful disease, characterized by progressive surface erosion of articular cartilage. The use of human articular chondrocytes (hACs) sourced from OA patients has been proposed as a potential therapy for cartilage repair, but this approach is limited by the lack of scalable methods to produce clinically relevant quantities of cartilage-generating cells. Previous studies in static culture have shown that hACs co-cultured with human mesenchymal stem cells (hMSCs) as 3D pellets can upregulate proliferation and generate neocartilage with enhanced functional matrix formation relative to that produced from either cell type alone...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28752541/functionalization-of-porous-bcp-scaffold-by-generating-cell-derived-extracellular-matrix-from-rat-bone-marrow-stem-cells-culture-for-bone-tissue-engineering
#8
Boram Kim, Reiza Ventura, Byong-Taek Lee
The potential of decellularized cell-derived ECM deposited on biphasic calcium phosphate (BCP) scaffold for bone tissue engineering was investigated. Rat derived bone marrow mesenchymal stem cells (RBMSCs) were cultured on porous BCP scaffolds for 3 weeks and decellularized with two different methods (freeze-thaw (F/T) or sodium dodecyl sulfate (SDS)). The decellularized ECM deposited scaffolds (dECM-BCP) were characterized through scanning electron microscopy, energy dispersive X-ray spectrometer, and confocal microscopy...
July 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28752652/y-27632-simplifies-the-isolation-procedure-of-human-primary-epidermal-cells-by-selectively-blocking-focal-adhesion-of-dermal-cells
#9
Jie Wen, Tingjian Zu, Qian Zhou, Xue Leng, Xunwei Wu
Primary skin epidermal cells isolation and in vitro expansion culture has been widely used for laboratory research and clinical applications. The conventional methods involving sequential enzymatic digestion of adult tissues have given low cell recovery rate and reduced cell viability. We report here an advanced method for human primary epidermal progenitor cells isolation from skin tissues including the Rho kinase inhibitor Y-27632. Compared to traditional protocols, the current protocol is simple, easy and faster; moreover, it gives a greater yield of integrin-expressing epithelial stem cells...
July 27, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28719946/cell-assisted-lipotransfer-friend-or-foe-in-fat-grafting-systematic-review-and-meta-analysis
#10
REVIEW
J Laloze, A Varin, J Gilhodes, N Bertheuil, J L Grolleau, J Brie, J Usseglio, L Sensebe, T Filleron, B Chaput
BACKGROUND: Autologous fat grafting is a common procedure for soft-tissue reconstruction but is associated with a graft resorption rate ranging from 20% to 80%. To improve the fat graft survival rate, a new technique, called cell-assisted lipotransfer (CAL), was developed. With CAL, fat is injected along with adipose-derived stromal cells (ASCs) that are assumed to improve fat survival rate. We conducted an evidence-based meta-analysis to evaluate the efficacy and safety of CAL as compared with conventional autologous fat grafting (non-CAL)...
July 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28719734/tissue-engineering-of-a-composite-trachea-construct-using-autologous-rabbit-chondrocytes
#11
James E Dennis, Kristina G Bernardi, Thomas J Kean, Nelson E Liou, Tanya K Meyer
The repair of large tracheal segmental defects remains an unsolved problem. The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements. Engineered tracheal replacements composed of autologous cells ("neotracheas") were tested in a New Zealand White rabbit model. Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle and an engineered cartilage sheet, and allowing the construct to mature for 8-12 weeks...
July 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715614/intraperitoneal-administration-of-adipose-tissue-derived-stem-cells-for-the-rescue-of-retinal-degeneration-in-a-mouse-model-via-indigenous-cntf-up-regulation-by-il-6
#12
Jeong Hoon Heo, Jung Ae Yoon, Eun Kyung Ahn, Hyun Kim, Sang Hwa Urm, Chul Oh Oak, Byeng Chul Yu, Sang Joon Lee
As the world's population begins to age, retinal degeneration is an increasing problem, and various treatment modalities are being developed. However, there have been no therapies for degenerative retinal conditions that are not characterized by neovascularization. We investigated whether transplantation of mouse adipose tissue-derived stem cells (mADSC) into the intraperitoneal space has a rescue effect on NaIO3 -induced retinal degeneration in mice. In this study, mADSC transplantation recovered visual function and preserved the retinal outer layer structure compared to the control group without any integration of mADSC into the retina...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715156/neutrophil-mediated-enhancement-of-angiogenesis-and-osteogenesis-in-a-novel-triple-cell-co-culture-model-with-endothelial-cells-and-osteoblasts
#13
T D K Herath, Anis Larbi, Swee Hin Teoh, C James Kirkpatrick, Bee Tin Goh
Repair and regeneration of critical-sized bone defects remain a major challenge in orthopaedic and craniomaxillofacial surgery. Until now, attempts to bioengineer bone tissue have been hindered by the inability to establish proper angiogenesis and osteogenesis in the tissue construct. In the present study, we established a novel triple cell co-culture model consisting of osteoblasts, endothelial cells, and neutrophils and conducted a systematic investigation of the effects of neutrophils on angiogenesis and osteogenesis...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715143/repetitive-short-span-application-of-extracellular-calcium-is-osteopromotive-to-osteoprogenitor-cells
#14
Hyungjin Jung, Thomas Mbimba, Mustafa Unal, Ozan Akkus
Extracellular calcium ([Ca(2+) ]E ) concentration has been suggested to stimulate osteoblastic activity; thus, calcium can be used to enhance fracture healing. However, systemic administration of calcium at high dose levels may cause physiological problems such as hypercalcemia. Short span application of single or repetitive [Ca(2+) ]E stimulus may be suggested as a novel regimen to reduce such side effects. However, osteopromotive effect of such short term [Ca(2+) ]E stimulus on osteoprogenitor cells has not yet been evaluated yet...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715139/uvb-exposure-of-a-humanized-skin-model-reveals-unexpected-dynamic-of-keratinocyte-proliferation-and-wnt-inhibitor-balancing
#15
Teresa Michalczyk, Thomas Biedermann, Sophie Böttcher-Haberzeth, Agnes S Klar, Martin Meuli, Ernst Reichmann
We developed human dermo-epidermal skin substitutes which are presently applied in phase I and II clinical trials. Here we used these very same skin equivalents containing melanocytes, named MelSkin, as an experimental skin model. We investigated the effects of UVB irradiation on the skin grafts transplanted on immune-compromised rats. The irradiation induces a strong wound healing response going along with massive proliferation of basal keratinocytes, basically quiescent under non-irradiated, homeostatic conditions...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28715113/calcium-deposition-in-photocrosslinked-poly-pro-hyp-gly-hydrogels-encapsulated-rat-bone-marrow-stromal-cells
#16
Farah Nurlidar, Keisuke Yamane, Mime Kobayashi, Kayo Terada, Tsuyoshi Ando, Masao Tanihara
Reproducing the features of the extracellular matrix is important for fabricating three-dimensional (3D) scaffolds for tissue regeneration. A collagen-like polypeptide, poly(Pro-Hyp-Gly), is a promising material for 3D scaffolds because of its excellent physical properties, biocompatibility, and biodegradability. In this paper, we present a novel photocrosslinked poly(Pro-Hyp-Gly) hydrogel as a 3D scaffold for simultaneous rat bone marrow stromal cell (rBMSC) encapsulation. The hydrogels were fabricated using visible-light photocrosslinking at various concentrations of methacrylated poly(Pro-Hyp-Gly) (20-50 mg/mL) and irradiation times (3 or 5 min)...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714574/a-humanised-tissue-engineered-bone-model-allows-species-specific-breast-cancer-related-bone-metastasis-in-vivo
#17
V M C Quent, A V Taubenberger, J C Reichert, L C Martine, J A Clements, D W Hutmacher, D Loessner
Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer-related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the complex cancer cell-bone interactions. In this study, two established tissue-engineered bone constructs (TEBCs) were applied to a breast cancer-related metastasis model. A cylindrical medical-grade polycaprolactone-tricalcium phosphate scaffold produced by fused deposition modelling (scaffold 1) was compared with a tubular calcium phosphate-coated polycaprolactone scaffold fabricated by solution electrospinning (scaffold 2) for their potential to generate ectopic humanised bone in NOD/SCID mice...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714570/human-osteoarthritic-chondrons-outnumber-patient-and-joint-matched-chondrocytes-in-hydrogel-culture-future-application-in-autologous-cell-based-oa-cartilage-repair
#18
Miriam Rothdiener, Tatiana Uynuk-Ool, Norbert Südkamp, Matthias Aurich, Alan J Grodzinsky, Bodo Kurz, Bernd Rolauffs
Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA-chondrons may outperform OA-chondrocytes. We compared patient- and joint surface-matched human OA-chondrons vs. OA-chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, collagen II synthesis (CPII) and degradation (C2C), and sGAG...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714568/exposure-to-reversine-affects-the-chondrocyte-morphology-and-phenotype-in-vitro
#19
S Gasparini, F Villa, L Molfetta, E Repaci, P Castagnola, R Quarto, P Giannoni
Articular chondrocytes derived from osteoarthritic tissues (OA-HAC) show a severely reduced chondrogenic commitment. This impairment undermines their use for tissue-engineered cartilage repair, which relies on cell proliferation and growth to meet the therapeutic needs but also on efficient cell plasticity to recover the chondrogenic phenotype. Reversine (Rev), a 2,6-disubstituted purine inhibitor of spindle-assembly checkpoints, was described to convert differentiated mesenchymal cells to their undifferentiated precursors...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714550/optimal-timing-and-frequency-of-bone-marrow-soup-therapy-for-functional-restoration-of-salivary-gland-injured-by-single-dose-or-fractionated-irradiation
#20
Dongdong Fang, Sixia Shang, Younan Liu, Mohammed Bakkar, Yoshinori Sumita, Jan Seuntjens, Simon D Tran
Injections of bone marrow (BM) cell extract, known as "BM Soup", was previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However the optimal starting time and frequency to maintain BM Soup therapeutic efficacy remains unknown. This study tests the optimal starting time and frequency of BM Soup injections in mice radiated with either a single dose or fractionated dose. First, BM Soup treatment is started at 1, 3, or 7 weeks post-IR, while positive (non-IR) and negative (IR) control mice receive injections of saline (vehicle control)...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
journal
journal
41661
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"