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Journal of Tissue Engineering and Regenerative Medicine

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https://www.readbyqxmd.com/read/28401697/establishment-of-novel-therapy-to-reduce-progression-of-myopia-in-rats-with-experimental-myopia-by-fibroblast-transplantation-on-sclera
#1
Kosei Shinohara, Takeshi Yoshida, Hongding Liu, Shizuko Ichinose, Tomoka Ishida, Ken-Ichi Nakahama, Natsuko Nagaoka, Muka Moriyama, Ikuo Morita, Kyoko Ohno-Matsui
Myopia is one of the most common visual disorders, and is characterized by a progressive axial elongation of the eye. Several methods have been tried to reduce the progression of axial elongation and myopia, but there are still no well-accepted procedures. We hypothesized that transplantation of fibroblasts on the sclera would lead to the synthesis of collagen fibrils on the sclera and reinforce it, and reduce the degree of axial elongation of eyes with form deprivation myopia. To examine this, we developed a form deprivation myopia model in albino Wistar rats and examined the effects of human fibroblasts (hFbs) transplantation on the sclera in the progression of myopia and axial elongation...
April 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28397402/micrornas-can-effectively-induce-formation-of-insulin-producing-cells-from-mesenchymal-stem-cells
#2
Chunyu Bai, Yuhua Gao, Xiangchen Li, Kunfu Wang, Hui Xiong, Zhiqiang Shan, Ping Zhang, Wenjie Wang, Weijun Guan, Yuehui Ma
MicroRNAs regulate insulin secretion, pancreatic development and beta cell differentiation. However, the function of microRNAs in the formation of insulin-producing cells (IPCs) from adult stem cells is poorly understood. We examine the microRNA expression profile in nestin-positive umbilical cord-derived mesenchymal stem cells (N-UCMSCs) and nestin-positive pancreatic mesenchymal stem cells using a deep sequencing approach. We also selected specific microRNAs for overexpression in N-UCMSCs and found that miR-375 and miR-26a induced IPCs differentiation from N-UCMSCs by downregulating target genes including mtpn, sox6, bhlhe22 and ccnd1...
April 10, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28388010/comparison-of-atmp-gingiva-and-skin-substitutes-and-their-in-vitro-wound-healing-potentials
#3
Mireille A Boink, Sanne Roffel, Melanie Breetveld, Maria Thon, Michiel S P Haasjes, Taco Waaijman, Rik J Scheper, Chantal S Blok, Susan Gibbs
Skin and oral mucosa substitutes are a therapeutic option for closing hard-to-heal skin and oral wounds. Our aim was to develop bi-layered skin and gingiva substitutes, from 3 mm diameter biopsies, cultured under identical conditions, which are compliant with current European regulations for advanced therapy medicinal products. We present in vitro mode of action methods to i) determine viability: epithelial expansion, proliferation (Ki-67), metabolic activity (MTT-assay); ii) characterize skin and gingiva substitutes: histology and immunohistochemistry; and iii) determine potency: soluble wound healing mediator release (ELISA)...
April 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28382732/engineering-vascularized-flaps-using-adipose-derived-microvascular-ec-and-msc
#4
Alina Freiman, Yulia Shandalov, Dekel Rosenfeld, Erez Shor, Dror Ben-David, Shai Meretzki, Shulamit Levenberg, Dana Egozi
Human adipose-derived microvascular endothelial cells (HAMEC) and mesenchymal stem cells (MSC) have been shown to bear angiogenic and vasculogenic capabilities. We hypothesize that co-culturing HAMEC:MSC on a porous biodegradable scaffold in vitro, later implanted as a graft around femoral blood vessels in a rat, will result in its vascularization by host vessels, creating a functional vascular flap that can effectively treat a range of large full-thickness soft tissue defects. HAMEC were co-cultured with MSC on polymeric 3D porous constructs...
April 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28382727/scalable-stirred-suspension-culture-for-the-generation-of-billions-of-human-induced-pluripotent-stem-cells-using-single-use-bioreactors
#5
Chee Keong Kwok, Yuichiro Ueda, Asifiqbal Kadari, Katharina Günther, Antoine Heron, Aletta C Schnitzler, Martha Rook, Frank Edenhofer
The production of human induced pluripotent stem cells (hiPSCs) in quantities that are relevant for cell-based therapies and cell-loaded implants through standard adherent culture is hardly achievable and lacks process scalability. A promising approach to overcoming these hurdles is the culture of hiPSCs in suspension. In this study, stirred suspension culture vessels were investigated for their suitability in the expansion of two hiPSC lines inoculated as a single cell suspension, with a free scalability between volumes of 50 and 2 400 mL...
April 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28374578/review-bioreactor-design-towards-generation-of-relevant-engineered-tissues-focus-on-clinical-translation
#6
REVIEW
Akhilandeshwari Ravichandran, Yuchun Liu, Swee-Hin Teoh
In tissue engineering and regenerative medicine, studies that utilize 3D scaffolds for generating voluminous tissues are mostly confined in the realm of in vitro research and preclinical animal model testing. Bioreactors offer an excellent platform to grow and develop 3D tissues by providing conditions that mimic their native microenvironment. Aligning the bioreactor development process with a focus on patient care will aid in the faster translation of the bioreactor technology to clinics. In this review, we discuss the various factors involved in the design of clinically relevant bioreactors in relation to their respective applications...
April 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28371514/bi-layer-silk-fibroin-grafts-support-functional-esophageal-repair-in-a-rodent-model-of-caustic-injury
#7
Khalid Algarrahi, Debra Franck, Alyssa Savarino, Vivian Cristofaro, Xuehui Yang, Saif Affas, Frank-Mattias Schäfer, Maryrose P Sullivan, Carlos R Estrada, Joshua R Mauney
Surgical repair of caustic esophageal injuries with autologous gastrointestinal segments is often associated with dysmotility, dysphagia, and donor site morbidity and therefore alternative graft options are needed. Bi-layer silk fibroin (BLSF) scaffolds were assessed for their ability to support functional restoration of damaged esophageal tissues in a rat model of onlay esophagoplasty. Transient exposure of isolated esophageal segments with 40% NaOH led to corrosive esophagitis and a 91% reduction in the luminal cross-sectional area of damaged sites...
March 30, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28371409/the-geometrical-shape-of-mesenchymal-stromal-cells-measured-by-quantitative-shape-descriptors-is-determined-by-the-stiffness-of-the-biomaterial-and-by-cyclic-tensile-forces
#8
Tatiana Uynuk-Ool, Miriam Rothdiener, Brandan Walters, Miriam Hegemann, Julian Palm, Phong Nguyen, Tanja Seeger, Ulrich Stöckle, Jan P Stegemann, Wilhelm K Aicher, Bodo Kurz, Melanie L Hart, Gerd Klein, Bernd Rolauffs
Controlling mesenchymal stromal cell (MSC) shape is a novel method for investigating and directing MSC behaviour in vitro. it was hypothesized that specifigc MSC shapes can be generated by using stiffness-defined biomaterial surfaces and by applying cyclic tensile forces. Biomaterials used were thin and thick silicone sheets, fibronectin coating, and compacted collagen type I sheets. The MSC morphology was quantified by shape descriptors describing dimensions and membrane protrusions. Nanoscale stiffness was measured by atomic force microscopy and the expression of smooth muscle cell (SMC) marker genes (ACTA2, TAGLN, CNN1) by quantitative reverse-transcription polymerase chain reaction...
March 29, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28342296/control-of-osmotic-pressure-to-improve-cell-viability-in-cell-laden-tissue-engineering-constructs
#9
A F Carvalho, L Gasperini, R S Ribeiro, A P Marques, R L Reis
Design of tissue engineering strategies deals with the need to balance both biomaterials characteristics and techniques specificities, often resulting in cell-compromising processing conditions. One important factor often disregarded is the osmotic pressure to which cells are exposed to. An in-house microfluidic system was used to prove that addition of an osmotic regulator significantly benefits the generation of viable cell-laden hydrogels under harsh processing conditions. Human adipose-derived stem cells (hASCs) were resuspended in 1...
March 25, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28326684/collagen-cellulose-nanocrystal-scaffolds-containing-curcumin-loaded-microspheres-on-infected-full-thickness-burns-repair
#10
Rui Guo, Yong Lan, Wei Xue, Biao Cheng, Yuanming Zhang, Changyong Wang, Seeram Ramakrishna
Burn infection is a serious problem that delays wound healing and leads to death. Curcumin (Cur) has been shown to exhibit antioxidant, anti-inflammatory, antimicrobial and anticarcinogenic activity. However, its instability, extremely low aqueous solubility and bioavailability in physiological fluids may make it difficult to maintain local Cur concentrations above the minimum inhibitory concentration for burn infection treatment. The objective of this study was to construct complexes of Cur/gelatin microspheres (GMs) and porous collagen (Coll)-cellulose nanocrystals (CNCs) composite scaffolds for full-thickness burn infection treatment...
March 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28326683/restoring-full-thickness-defects-with-spray-skin-in-conjunction-with-dermal-regenerate-template-and-split-thickness-skin-grafting-a-pilot-study
#11
Daniel Hammer, Juan L Rendon, Jennifer Sabino, Kerry Latham, Mark E Fleming, Ian L Valerio
Complex, full-thickness soft tissue defects secondary to large burns, trauma and war-related injuries continue to challenge reconstructive surgeons. To achieve positive surgical outcomes in these patient populations, novel approaches are needed to restore the functional, protective and aesthetic properties of skin. Herein, we provide the first report describing the staged use of a dermal regenerate template (DRT) with a spray-on epidermal regenerative modality (spray skin) in addition to autologous split-thickness skin grafting (STSG) in non-burn trauma and compare these results with those of patients treated with DRT and STSG alone...
March 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28317340/production-of-pancreatic-progenitor-cells-from-human-induced-pluripotent-stem-cells-using-a-three-dimensional-suspension-bioreactor-system
#12
Yuichiro Mihara, Katsuhisa Matsuura, Yoshihiro Sakamoto, Teruo Okano, Norihiro Kokudo, Tatsuya Shimizu
Islet replacement is a promising strategy for the treatment of patients with type 1 diabetes and patients who have undergone total pancreatectomy. Recent progress in cellular reprogramming technology may allow the transplantation of a patient's own pancreatic cells. Although many studies have reported the differentiation of pancreatic progenitor cells from mouse and human pluripotent stem cells (PSCs), obtaining sufficient cell numbers for clinical applications remains problematic. Here, we describe the mass production of human pancreatic progenitor cells from human induced (i)PSCs using a three-dimensional suspension bioreactor system...
March 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28296347/gdnf-schwann-cells-in-hydrogel-scaffolds-promote-regional-axon-regeneration-remyelination-and-functional-improvement-after-spinal-cord-transection-in-rats
#13
Bingkun K Chen, Nicolas N Madigan, Jeffrey S Hakim, Mahrokh Dadsetan, Siobhan S McMahon, Michael J Yaszemski, Anthony J Windebank
Positively-charged oligo[poly(ethylene glycol)fumarate] (OPF(+) ) is a biodegradable hydrogel used for spinal cord injury repair. We compared scaffolds containing primary Schwann cells (SCs) to scaffolds delivering SCs genetically modified to secrete high concentrations of glial cell-derived neurotrophic factor (GDNF). Multichannel OPF(+) scaffolds loaded with SCs or GDNF-SCs were implanted into transected rat spinal cords for 4 weeks. GDNF-SCs promoted regeneration of more axons into OPF(+) scaffolds (2773...
March 10, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28256798/the-roles-and-perspectives-of-micrornas-as-biomarkers-for-intervertebral-disc-degeneration
#14
REVIEW
Xiaoya Zhou, Lili Chen, Sibylle Grad, Mauro Alini, Haobo Pan, Dazhi Yang, Wanxin Zhen, Zhizhong Li, Shishu Huang, Songlin Peng
MicroRNAs (miRNAs) are highly conserved molecules that regulate protein levels post-transcriptionally. Aberrant miRNA expression presents in various musculoskeletal disorders, such as osteoporosis, osteoarthritis and rheumatoid arthritis. The expression levels of miRNAs are characterized by endogenous properties and tissue specificity. This raises the possibility that miRNAs could serve as useful clinical biomarkers in the diagnosis of certain diseases. Intervertebral disc degeneration (IDD) is one of the major causes of back pain, and a process characterized by a cascade of molecular, cellular, biochemical and structural changes...
March 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28244684/sustained-release-of-parathyroid-hormone-via-in-situ-crosslinking-gelatin-hydrogels-improves-the-therapeutic-potential-of-tonsil-derived-mesenchymal-stem-cells-for-hypoparathyroidism
#15
Yoon Shin Park, Yunki Lee, Yoon Mi Jin, Gyungah Kim, Sung Chul Jung, Yoon Jeong Park, Ki Dong Park, Inho Jo
Biomimetic parathyroid regeneration with sustained release of parathyroid hormone (PTH) into the blood stream is a considerable challenge in hypoparathyroidism treatment. We recently reported that tonsil-derived mesenchymal stem cells (TMSC), if these cells were both differentiated in vitro before implantation and incorporated into a scaffold Matrigel, are a good cell source for parathyroid regeneration in a parathyroidectomized (PTX) animal model. Here, we present a new strategy for improved clinical application that enhances the sustained release of PTH by controlling mechanical stiffness using in situ-forming gelatin-hydroxyphenyl propionic acid (GH) hydrogels (GHH)...
February 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28211195/a-comparison-of-the-new-japanese-expedited-approval-legislation-for-regenerative-medicine-product-with-the-existing-systems-of-the-united-states-and-european-union
#16
Yoji Jokura, Kazuo Yano, Masayuki Yamato
Expedited approval pathways/programs for drugs, biologics, or medical devices have been enacted for rapid product commercialization of innovative products in the United States (US) and European Union (EU). However, less innovative products are increasingly using these expedited pathways and frequently obligations to collect and report post-marketing data on approved products are being bypassed. The Japanese government recently enacted a new conditional and time-limited approval pathway dedicated to regenerative medicine products...
February 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28198165/vascular-endothelial-growth-factor-transfected-adipose-derived-stromal-cells-enhance-bone-regeneration-and-neovascularization-from-bone-marrow-stromal-cells
#17
Mi-Lan Kang, Ji-Eun Kim, Gun-Il Im
Vascular endothelial growth factor (VEGF)-transfected adipose-derived stromal cells (ADSCs(VEGF) ) were devised to promote bone regeneration and neovascularization of bone marrow stromal cells (BMSCs). ADSCs(VEGF) were added to BMSCs and cocultured in variable proportions. ADSCs(VEGF) alone or ADSCs(VEGF) with BMSCs (BMSCs:ADSCs(VEGF) ratio of 1:0.025-0.5) induced significantly greater tube formation in human umbilical vein endothelial cells than untransfected ADSCs. The cocultures of BMSCs and ADSCs(VEGF) at ratios of 1: 0...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28198149/the-effects-of-using-vitrified-chondrocyte-sheets-on-pain-alleviation-and-articular-cartilage-repair
#18
Yoshiki Tani, Masato Sato, Miki Maehara, Hiroshi Nagashima, Munetaka Yokoyama, Miyuki Yokoyama, Masayuki Yamato, Teruo Okano, Joji Mochida
The effect of using vitrified-thawed chondrocyte sheets on articular cartilage repair was examined because the methods for storing chondrocyte sheets are essential for allogeneic chondrocyte sheet transplantation. Six Japanese white rabbits were used as sources of articular chondrocytes and synovial cells. Chondrocytes were harvested from the femur, and synovial cells were harvested from inside the knee joints. After coculture of the chondrocytes with synovial cells, triple-layered chondrocyte sheets were fabricated...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28198096/treated-dentin-matrix-paste-as-a-novel-pulp-capping-agent-for-dentin-regeneration
#19
Jinlong Chen, Caiyun Cui, Xiangchen Qiao, Bo Yang, Mei Yu, Weihua Guo, Weidong Tian
Regenerating dentin and preserving pulp vitality are the two key targets for the treatment of dental pulp exposure. Calcium hydroxide (CH), the widely used capping agent, may induce potential tunnel defect in reparative dentin and cause inflammation or even necrosis in pulp tissues. This study aimed to produce a novel pulp capping agent with better bioactivities. Treated dentin matrix (TDM) paste (TDMP) was fabricated consisting of TDM powder and aqueous TDM extract. The chemical and biological characteristics of TDMP were investigated, and its effect on the odontogenic differentiation of dental pulp stem cells explored at gene and protein level; the therapeutic effect for pulp exposure in miniature swine was further verified...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28194878/platelet-activated-serum-might-have-a-therapeutic-effect-on-damaged-articular-cartilage
#20
Munetaka Yokoyama, Masato Sato, Yoshiki Tani, Miyuki Yokoyama, Mami Kokubo, Masayuki Yamato, Teruo Okano, Joji Mochida
Platelet-activated serum (PAS) was collected from rabbits. This contains high concentrations of growth factors, including vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF)-BB, and transforming growth factor-beta (TGF-β). PAS was injected into the knee joints of Japanese White rabbits subjected to anterior cruciate ligament transection (ACL-T) to investigate its therapeutic effects on articular cartilage. The effect of Avastin (an anti-VEGF monoclonal antibody) on VEGF expression was also investigated...
February 14, 2017: Journal of Tissue Engineering and Regenerative Medicine
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