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Journal of Infection in Developing Countries

Eda Kepenekli Kadayifci, Ayşe Karaaslan, Kenan Midilli, Ahmet Soysal, Mustafa Bakır
No abstract text is available yet for this article.
September 30, 2016: Journal of Infection in Developing Countries
Valerio Iebba, Floriana Santangelo, Valentina Totino, Fabrizio Pantanella, Anatole Monsia, Veronica Di Cristanziano, David Di Cave, Serena Schippa, Federica Berrilli, Rossella D'Alfonso
INTRODUCTION: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d'Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. METHODOLOGY: Twenty fecal samples were collected from people inhabiting three different localities of Côte d'Ivoire for copromicroscopic analysis and molecular identification of G...
September 30, 2016: Journal of Infection in Developing Countries
Jie Pan, Xin Li, Guowei He, Shuai Yuan, Pingfeng Feng, Xiuming Zhang, Yurong Qiu
INTRODUCTION: Fast screening tests for hepatitis C virus (HCV) antibody often give false-positive results. Signal-to-cut-off (S/Co) ratios were suggested to be used as reflex confirmation of anti-HCV. The Elecsys Anti-HCV II assay is an effective test for the detection of hepatitis C, but no S/Co cutoff has been reported. The aim of this study was to determine the S/Co ratio threshold of anti-HCV test using Elecsys Anti-HCV II screening and supplemental recombinant immunoblot assay (RIBA) test results as the gold standard...
September 30, 2016: Journal of Infection in Developing Countries
Huijuan Zhu, Xingxiang Liu, Yi Ding, Hui Zhou, Yiying Wang, Zhiwei Zhou, Xinxin Li, Zengli Zhang, Chen Dong
INTRODUCTION: Vitamin D is significantly associated with virus replication in chronic hepatitis B virus (HBV) infection. However, the relationship between low serum vitamin D levels and HBV "a" determinant mutations remains unknown. METHODOLOGY: A total of 133 chronically HBV-infected, treatment-naive patients were randomly selected in the present study. Serum vitamin D levels were measured by using liquid chromatography-mass spectrometry. The HBV "a" determinant was amplified, sequenced, and analyzed by nested polymerase chain reaction (PCR)...
September 30, 2016: Journal of Infection in Developing Countries
Nessma Ahmed El Zawawy, Sameh Samir Ali
INTRODUCTION: Protease enzyme is considered one of the most serious virulence factors produced by extended-spectrum β-lactamase-producing and multidrug-resistant Pseudomonas aeruginosa (ESβLMDRPA) clinical isolates. METHODOLOGY: The antibacterial activity of methanol extract of Ganoderma lucidum fruiting bodies was tested against a protease-producing ESβLMDRPA clinical isolate, showing its mode of action. RESULTS: The extract showed high antibacterial activity...
September 30, 2016: Journal of Infection in Developing Countries
Fabio Macchioni, Higinio Segundo, Valentina Totino, Simona Gabrielli, Patricia Rojas, Mimmo Roselli, Grover Adolfo Paredes, Mario Masana, Alessandro Bartoloni, Gabriella Cancrini
INTRODUCTION: In 2013 a coproparasitological survey was carried out in two rural communities of the Bolivian Chaco to determine the prevalence of intestinal parasitic infections (IPIs) and to investigate on possible infection drivers through a questionnaire interview. METHODOLOGY: Faecal samples were examined by microscopy. Samples positive for Entamoeba histolytica complex and Blastocystis were molecularly examined to identify the species/subtypes involved. RESULTS: The overall infection rate was 86%, identical in both communities and mostly due to protozoa...
September 30, 2016: Journal of Infection in Developing Countries
Essam Mohammed Janahi, Sakina Mustafa, Sajeda Alsari, Mariam Al-Mannai, Ghada N Farhat
INTRODUCTION: HIV/AIDS is one of the major health problems worldwide. Despite the low prevalence of HIV in Bahrain, educational and awareness programs remain highly important in controlling and preventing the spread of the disease. This study aimed to assess the public's knowledge, risk perceptions, and attitudes towards HIV/AIDS in Bahrain. METHODOLOGY: A self-administered questionnaire-based survey was administered to and completed by 1,038 Bahraini adults. RESULTS: Although the average general awareness among participants was good (63%), some misconceptions and erroneous beliefs were common, including knowledge of mode of transmission and high risk groups...
September 30, 2016: Journal of Infection in Developing Countries
Fuchu Qian, Jiqu Qin, Dongli Li, Zhihong Ma, Hairong Zhang, Fang Jin, Weihong Wang
INTRODUCTION: Antiviral drug-resistance patterns of hepatitis B virus (HBV) mutants are complex and currently partly understood. The aim of this study was to monitor the genotypic resistance profile in patients with chronic hepatitis B (CHB) receiving nucleos(t)ide analogues (NAs) in Huzhou, eastern China. METHODOLOGY: Serum samples of 139 CHB patients undergoing NA treatment were obtained from Huzhou Central Hospital. The full-length HBV reverse transcriptase regions were amplified and sequenced...
September 30, 2016: Journal of Infection in Developing Countries
Huong Thi Thanh Doan, Xuyen Thi Kim Le, Roan Thi Do, Chau Thi Minh Hoang, Khue Thi Nguyen, Thanh Hoa Le
INTRODUCTION: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009-2013. METHODOLOGY: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed. RESULTS: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3...
September 30, 2016: Journal of Infection in Developing Countries
Juan Carlos Domínguez-Hermosillo, José Antonio Mata-Marin, Norma Estela Herrera-González, Marcelino Chávez-García, Gloria Huerta-García, Nohemí Nuñez-Rodríguez, José Gerardo García-Gámez, Anai Jiménez-Romero, Jesús Enrique Gaytán-Martínez
INTRODUCTION: Although both tipranavir (TPV) and darunavir (DRV) represent important options for the management of patients with multi-protease inhibitor (PI)-resistant human immunodeficiency virus (HIV), currently there are no studies comparing the effectiveness and safety of these two drugs in the Mexican population. The aim of this study was to compare the effectiveness of TPV versus DRV as a salvage therapy in HIV-1 treatment-experienced patients. METHODOLOGY: This was a comparative, prospective, cohort study...
September 30, 2016: Journal of Infection in Developing Countries
Mingming Tan, Wenkui Sun, Chunlai Feng, Di Xia, Xiaoyue Shen, Yuan Ding, Zhicheng Liu, Zheng Xing, Xin Su, Yi Shi
INTRODUCTION: Evaluating the attenuation of influenza viruses in animal studies is important in developing safe and effective vaccines. This study aimed to demonstrate that the microRNA (miRNA)-let-7b-mediated attenuated influenza viruses (miRT-H1N1) are sufficiently attenuated and safe in mice. METHODOLOGY: The pathogenicity of the miRT-H1N1virus was investigated in a mouse model, evaluated with median lethal dose (LD50). The replicative dynamics of the miRT-H1N1, wild type (wt)-H1N1, and scramble (scbl)-H1N1 viruses in the lungs of infected mice were compared...
September 30, 2016: Journal of Infection in Developing Countries
Sinisa Skocibusic, Marko Martinac, Jurica Arapovic, Svjetlana Grgic, Jadranka Nikolic, Dino Hasanagic, Milenko Bevanda, Jelena Ravlija
INTRODUCTION: Use of intravenous heroin carries a risk of serious medical conditions, including acquiring blood-borne infections. Therefore, hepatitis B virus (HBV) and hepatitis C virus (HCV) infection represent a threat for people who inject drugs (PWID). The objectives of this study were to determine the extent and characteristics of risk factors for acquiring HBV and HCV infection in PWID included in opiate substitution treatment in the southern part of Bosnia and Herzegovina (B&H)...
September 30, 2016: Journal of Infection in Developing Countries
Ana Silva, Raquel M Moniz, Tânia Pereirinha, Maria J Brilhante, Sara Bulhões, Rita Cabral, Claudia C Branco, Luisa Mota-Vieira
INTRODUCTION: We performed a descriptive analysis of molecular diagnosis of infectious agents in the São Miguel Island population, in order to address questions like what is the frequency of clinical requests, is it observable seasonality of pathogens, and what is the positive rate for the clinical diagnosis. METHODOLOGY: This was a retrospective and descriptive study based on 878 individuals suspected of harboring infectious diseases during two consecutive years, 2012-2013...
September 30, 2016: Journal of Infection in Developing Countries
Soumaya Moustafa Hijazi, Mohamad Anwar Fawzi, Faten Moustafa Ali, Khaled Hussein Abd El Galil
INTRODUCTION: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) infections are a growing threat to children, and the treatment of these infections becomes more and more challenging. A huge reservoir for ESBLs in the community is the fecal flora of children. This study investigates the rectal colonization, associated risk factors, antimicrobial susceptibility, and molecular characterization of ESBL-PE in Lebanese community infants. METHODOLOGY: A total of 117 rectal swabs were taken from healthy infants between 1 and 12 months of age...
September 30, 2016: Journal of Infection in Developing Countries
Akm Anisur Rahman, Dirk Berkvens, Claude Saegerman, David Fretin, Noor Muhammad, Akram Hossain, Emmanuel Abatih
INTRODUCTION: This study describes the seroprevalence of human brucellosis among pyretic patients and detection of Brucella abortus DNA from seropositive pyretic patients using real-time polymerase chain reaction (rtPCR) for the first time in Bangladesh. METHODOLOGY: Blood samples were collected from 300 pyretic patients from October 2007 to May 2008 and subjected to three serological tests: Rose-Bengal plate test (RBT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (iELISA)...
September 30, 2016: Journal of Infection in Developing Countries
Myrella Cariry Lira, Paricia Emília Naves Givisiez, Francisca Geovânia Canafístula De Sousa, Marciane Magnani, Evandro Leite De Souza, Denis Augusto Spricigo, Wondwossen Abebe Gebreyes, Celso José Bruno De Oliveira
INTRODUCTION: Biofilm-associated antimicrobial resistance is of increasing importance to the maintenance and spread of foodborne pathogens in the food industry. This study aimed to investigate the ability to form biofilm and the antimicrobial resistance of staphylococci contaminating small-scale goat milk dairy plants. METHODOLOGY: Sixty isolates were tested for antimicrobial resistance against 20 drugs by the microdilution method. Biofilm-forming traits were assessed by the microtiter plate method (MtP), Congo red agar method (CRA), and icaD gene detection by polymerase chain reaction (PCR)...
September 30, 2016: Journal of Infection in Developing Countries
Eyitayo O Adenipekun, Charlene R Jackson, Hazem Ramadan, Bamidele A Iwalokun, Kolawole S Oyedeji, Jonathan G Frye, John B Barrett, Lari M Hiott, Tiffanie A Woodley, Afolabi Oluwadun
INTRODUCTION: The emergence of multidrug resistance (MDR; resistance to ≥ 2 more antimicrobials) in Escherichia coli is of concern due to complications encountered in treatment. METHODOLOGY: In this study, prevalence, antimicrobial resistance, and genetic characteristics of MDR community isolates of E. coli from Lagos, Nigeria were determined. Urine and stool samples were obtained from outpatients attending Lagos State hospitals and from animal handlers in abattoirs, poultries, and open markets, from December 2012 to July 2013...
September 30, 2016: Journal of Infection in Developing Countries
Daniele Maria Knupp de Souza, Claudio Galuppo Diniz, Didier Silveira Castellano Filho, Laura Maria Andrade de Oliveira, Débora Martins Coelho, Luciana De Souza Talha, Thiago César do Nascimento, Alessandra Barbosa Ferreira-Machado, Vânia Lúcia da Silva
INTRODUCTION: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients. METHODOLOGY: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests...
September 30, 2016: Journal of Infection in Developing Countries
Dragana Protic, Dragana Savic, Dragana Andjelkovic, Nina Djukanovic, Marija Zdravkovic, Sinisa F Djurasevic, Zoran Todorovic
INTRODUCTION: Coagulase-negative staphylococci (CoNS) are increasingly resistant nosocomial pathogens. We aimed to analyze the prevalence of CoNS isolates in clinical settings, the evolution of antimicrobial resistance of CoNS, and antibiotic consumption in a hospital. METHODOLOGY: This retrospective cohort study was carried out at a tertiary healthcare facility over 17 months. Identification of isolated cultures and antibiotic susceptibility testing were performed using the Vitek2 system...
September 30, 2016: Journal of Infection in Developing Countries
Luisa Moreira Silva, Willer Goncalves Dourado Santos, Mittermayer Barretto Santiago
The aim of this study was to review and evaluate systematically the scientific evidence on the relationship between systemic lupus erythematosus (SLE) and cutaneous warts (CW) caused by human papillomavirus (HPV) infection. With strict inclusion and exclusion criteria, we extensively searched the PUBMED, BVS (Virtual Health Library), and SCOPUS databases for the studies that evaluated the prevalence of CW in patients with SLE. Secondary references were additionally obtained from the selected articles. Only four articles met the research criteria and showed a higher frequency of CW in SLE patients compared to healthy controls...
September 30, 2016: Journal of Infection in Developing Countries
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