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Journal of Genetics and Genomics, Yi Chuan Xue Bao

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https://www.readbyqxmd.com/read/28416245/a-simple-and-efficient-method-for-crispr-cas9-induced-mutant-screening
#1
Yufeng Hua, Chun Wang, Jian Huang, Kejian Wang
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction (PCR)/restriction enzyme (RE) assay, T7 endonuclease I (T7EI) assay, Surveyor nuclease assay, PAGE-based genotyping assay, and high-resolution melting (HRM) analysis-based assay have been developed for screening CRISPR/Cas9-induced mutants. However, these methods are time- and labour-intensive and may also be sequence-limited or require very expensive equipment...
April 4, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28412228/temperature-effect-on-crispr-cas9-mediated-genome-editing
#2
Guanghai Xiang, Xingying Zhang, Chenrui An, Chen Cheng, Haoyi Wang
Zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR-Cas9) are the most commonly used genome editing tools. Previous studies demonstrated that hypothermia treatment increased the mutation rates induced by ZFNs and TALENs in mammalian cells. Here, we characterize the effect of different culture temperatures on CRISPR-Cas9 mediated genome editing and find that the genome editing efficiency of CRISPR-Cas9 is significantly hampered by hypothermia treatment, unlike ZFN and TALEN...
March 30, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28427862/molecular-evolution-of-pancreatic-ribonuclease-gene-rnase1-in-rodentia
#3
Da-Tian Lang, Xiao-Ping Wang, Lei Wang, Li Yu
No abstract text is available yet for this article.
March 19, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28325553/metabolomics-through-the-lens-of-precision-cardiovascular-medicine
#4
REVIEW
Sin Man Lam, Yuan Wang, Bowen Li, Jie Du, Guanghou Shui
Metabolomics, which targets at the extensive characterization and quantitation of global metabolites from both endogenous and exogenous sources, has emerged as a novel technological avenue to advance the field of precision medicine principally driven by genomics-oriented approaches. In particular, metabolomics has revealed the cardinal roles that the environment exerts in driving the progression of major diseases threatening public health. Herein, the existent and potential applications of metabolomics in two key areas of precision cardiovascular medicine will be critically discussed: 1) the use of metabolomics in unveiling novel disease biomarkers and pathological pathways; 2) the contribution of metabolomics in cardiovascular drug development...
March 8, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28325554/inscuteable-maintains-type-i-neuroblast-lineage-identity-via-numb-notch-signaling-in-the-drosophila-larval-brain
#5
Huanping An, Wanzhong Ge, Yongmei Xi, Xiaohang Yang
In the Drosophila larval brain, type I and type II neuroblasts (NBs) undergo a series of asymmetric divisions which give rise to distinct progeny lineages. The intermediate neural progenitors (INPs) exist only in type II NB lineages. In this study, we reveal a novel function of Inscuteable (Insc) that acts to maintain type I NB lineage identity. In insc type I NB clones of mosaic analyses with a repressible cell marker (MARCM), the formation of extra Deadpan (Dpn)(+) NB-like and GMC-like cells is observed. The lack of Insc leads to the defective localization and segregation of Numb during asymmetric cell division...
March 6, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28411032/a-2-bp-insertion-c-67_68inscc-in-mc1r-causes-recessive-white-coat-color-in-bama-miniature-pigs
#6
Qitao Jia, Chunwei Cao, Tang Hai, Ying Zhang, Qiantao Zheng, Xiao Wang, Rui Zhang, Xianlong Wang, Ailing Luo, Hong Wei, Anming Meng, Qi Zhou, Hongmei Wang, Jianguo Zhao
No abstract text is available yet for this article.
February 28, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28411033/significant-differences-of-function-and-expression-of-micrornas-between-ground-state-and-serum-cultured-pluripotent-stem-cells
#7
Ying Yan, Xi Yang, Ting-Ting Li, Kai-Li Gu, Jing Hao, Qiang Zhang, Yangming Wang
Serum- and 2i-cultured embryonic stem cells (ESCs) show different epigenetic landscapes and transcriptomic profiles. The difference in the function and expression of microRNAs (miRNAs) between these two states remains unclear. Here, we showed that 2i- and serum-cultured ESCs exhibited distinctive miRNA expression profiles with >100 miRNAs differentially expressed, and the expression changes were largely due to transcriptional regulation. We further characterized the function of miRNAs differentially expressed under two conditions and found that ESCs exhibited higher degree of dependency on miRNAs for rapid proliferation; since Dgcr8(-/-) or Dicer1(-/-) but not wild-type ESCs showed slower growth rate and more accumulation in the G1 phase under 2i than serum condition...
February 22, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28412227/generation-of-thermosensitive-male-sterile-maize-by-targeted-knockout-of-the-zmtms5-gene
#8
Jun Li, Huawei Zhang, Xiaomin Si, Youhui Tian, Kunling Chen, Jinxing Liu, Huabang Chen, Caixia Gao
No abstract text is available yet for this article.
February 21, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28302421/identification-and-analysis-of-intermediate-size-noncoding-rnas-in-the-rhesus-macaque-fetal-brain
#9
LETTER
Liyuan Zhu, Xiaochao Tan, Wei Liu, Fengbiao Mao, Chao Wu, Junjie Zhou, Xiao Liu, Shuaiyao Lu, Kaili Ma, Bin Yin, Jianjun Luo, Jiangang Yuan, Boqin Qiang, Runsheng Chen, Xiaozhong Peng
No abstract text is available yet for this article.
March 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28302420/a-cryptic-mitochondrial-dna-link-between-north-european-and-west-african-dogs
#10
Adeniyi C Adeola, Sheila C Ommeh, Jiao-Jiao Song, S Charles Olaogun, Oscar J Sanke, Ting-Ting Yin, Guo-Dong Wang, Shi-Fang Wu, Zhong-Yin Zhou, Jacqueline K Lichoti, Bernard R Agwanda, Philip M Dawuda, Robert W Murphy, Min-Sheng Peng, Ya-Ping Zhang
Domestic dogs have an ancient origin and a long history in Africa. Nevertheless, the timing and sources of their introduction into Africa remain enigmatic. Herein, we analyse variation in mitochondrial DNA (mtDNA) D-loop sequences from 345 Nigerian and 37 Kenyan village dogs plus 1530 published sequences of dogs from other parts of Africa, Europe and West Asia. All Kenyan dogs can be assigned to one of three haplogroups (matrilines; clades): A, B, and C, while Nigerian dogs can be assigned to one of four haplogroups A, B, C, and D...
March 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28298255/using-mutpred-derived-mtdna-load-scores-to-evaluate-mtdna-variation-in-hypertension-and-diabetes-in-a-two-population-cohort-the-sabpa-study
#11
Marianne Venter, Leone Malan, Etresia van Dyk, Joanna L Elson, Francois H van der Westhuizen
Mitochondrial DNA (mtDNA) variation has been implicated in many common complex diseases, but inconsistent and contradicting results are common. Here we introduce a novel mutational load hypothesis, which also considers the collective effect of mainly rare variants, utilising the MutPred Program. We apply this new methodology to investigate the possible role of mtDNA in two cardiovascular disease (CVD) phenotypes (hypertension and hyperglycaemia), within a two-population cohort (n = 363; mean age 45 ± 9 yrs)...
March 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28291639/high-efficiency-breeding-of-early-maturing-rice-cultivars-via-crispr-cas9-mediated-genome-editing
#12
LETTER
Xiufeng Li, Wenjia Zhou, Yuekun Ren, Xiaojie Tian, Tianxiao Lv, Zhenyu Wang, Jun Fang, Chengcai Chu, Jie Yang, Qingyun Bu
No abstract text is available yet for this article.
March 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28209302/neuronal-insulin-receptor-mediates-a-positive-feedback-regulation-of-insulin-biosynthesis-in-drosophila
#13
LETTER
Jie Ye, Danping Chen, Yue Yu, Liming Wang
No abstract text is available yet for this article.
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28209301/iswi-atp-dependent-remodeling-of-nucleoplasmic-%C3%AF-speckles-in-the-brain-of-drosophila-melanogaster
#14
Luca Lo Piccolo, Andrea Attardi, Rosa Bonaccorso, Lorenzo Li Greci, Giorgio Giurato, Antonia Maria Rita Ingrassia, Maria Cristina Onorati
Heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to the RNA-binding proteins family. They are involved in processing heterogeneous nuclear RNAs (hnRNAs) into mature mRNAs. These proteins participate in every step of mRNA cycle, such as mRNA export, localization, translation, stability and alternative splicing. At least 14 major hnRNPs, which have structural and functional homologues in mammals, are expressed in Drosophila melanogaster. Until now, six of these hnRNPs are known to be nucleus-localized and associated with the long non-coding RNA (lncRNA) heat shock responsive ω (hsrω) in the omega speckle compartments (ω-speckles)...
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28190776/regulators-of-alternative-polyadenylation-operate-at-the-transition-from-mitosis-to-meiosis
#15
Lingjuan Shan, Chan Wu, Di Chen, Lei Hou, Xin Li, Lixia Wang, Xiao Chu, Yifeng Hou, Zhaohui Wang
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted...
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28162959/ongene-a-literature-based-database-for-human-oncogenes
#16
LETTER
Yining Liu, Jingchun Sun, Min Zhao
No abstract text is available yet for this article.
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28162958/lmm5-1-and-lmm5-4-two-eukaryotic-translation-elongation-factor-1a-like-gene-family-members-negatively-affect-cell-death-and-disease-resistance-in-rice
#17
Jiying Zhao, Pengcheng Liu, Chunrong Li, Yanyan Wang, Lequn Guo, Guanghuai Jiang, Wenxue Zhai
Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5...
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28161325/mir-503-3p-promotes-epithelial-mesenchymal-transition-in-breast-cancer-by-directly-targeting-smad2-and-e-cadherin
#18
Zitong Zhao, Xinyi Fan, Lanfang Jiang, Zhongqiu Xu, Liyan Xue, Qimin Zhan, Yongmei Song
Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of effective therapeutic targets to prevent breast cancer metastasis is urgently needed. The function of miR-503-3p has been investigated in other cancers, but its role in breast cancer remains undefined. Here, we found that miR-503-3p was overexpressed in breast cancer tissue and plasma compared with adjacent normal breast tissue and with plasma from healthy individuals...
February 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28160970/editorial
#19
EDITORIAL
Yongbiao Xue
No abstract text is available yet for this article.
January 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28117224/drug-resistance-mechanisms-and-novel-drug-targets-for-tuberculosis-therapy
#20
REVIEW
Md Mahmudul Islam, H M Adnan Hameed, Julius Mugweru, Chiranjibi Chhotaray, Changwei Wang, Yaoju Tan, Jianxiong Liu, Xinjie Li, Shouyong Tan, Iwao Ojima, Wing Wai Yew, Eric Nuermberger, Gyanu Lamichhane, Tianyu Zhang
Drug-resistant tuberculosis (TB) poses a significant challenge to the successful treatment and control of TB worldwide. Resistance to anti-TB drugs has existed since the beginning of the chemotherapy era. New insights into the resistant mechanisms of anti-TB drugs have been provided. Better understanding of drug resistance mechanisms helps in the development of new tools for the rapid diagnosis of drug-resistant TB. There is also a pressing need in the development of new drugs with novel targets to improve the current treatment of TB and to prevent the emergence of drug resistance in Mycobacterium tuberculosis...
January 20, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
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