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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/29692558/identification-of-candidate-resistance-genes-of-cotton-against-aspergillus-flavus-infection-using-a-comparative-transcriptomics-approach
#1
Muthamilarasan Mehanathan, Renesh Bedre, Venkata Mangu, Kanniah Rajasekaran, Deepak Bhatnagar, Niranjan Baisakh
A comparative transcriptome analysis was performed using the genes significantly differentially expressed in cotton, corn and peanut in response to aflatoxin producing fungus Aspergillus flavus with an objective of identifying candidate resistance genes in cotton. Two-way analyses identified 732 unique genes to be differentially regulated by the fungus with only 26 genes common across all three crops that were considered candidate A. flavus resistance genes with an assumption that these genes have specific roles in conferring the resistance trait...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692557/agroinfiltration-a-rapid-and-reliable-method-to-select-suitable-rose-cultivars-for-blue-flower-production
#2
Masoume Zeinipour, Pejman Azadi, Ahmad Majd, Maryam Jafarkhani Kermani, Saeed Irian, Seyed Mohammad Hosseini, Masahiro Mii
Rose cultivars with blue flower color are among the most attractive breeding targets in floriculture. However, they are difficult to produce due to the low efficiency of transformation systems, interactive effects of hosts and vectors, and lengthy processes. In this study, agroinfiltration-mediated transient expression was investigated as a tool to assess the function of flower color genes and to determine appropriate host cultivars for stable transformation in Rosa hybrida . To induce delphinidin accumulation and consequently to produce blue hue, the petals of 30 rose cultivars were infiltrated with three different expression vectors namely pBIH-35S-CcF3'5'H, pBIH-35S-Del2 and pBIH-35S-Del8, harbouring different sets of flower color genes...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692556/assessment-of-genetic-diversity-and-population-structure-in-gladiolus-gladiolus-hybridus-hort-by-issr-markers
#3
Veena Chaudhary, Mukesh Kumar, Shailendra Sharma, Navneet Kumar, Vipin Kumar, Hemant Kumar Yadav, Shiveta Sharma, Ujjwal Sirohi
ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus ( Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692555/genetic-diversity-and-structure-of-capparis-spinosa-l-in-iran-as-revealed-by-issr-markers
#4
Maryam Ahmadi, Hojjatollah Saeidi
Capparis spinosa L. (caper bush) is an economically and ecologically important perennial shrub that grows across different regions of Iran. In this study, the genetic diversity and population structure of Iranian genepool of C. spinosa is evaluated using Inter Simple Sequence Repeat (ISSR) markers. Using 10 ISSR primers, 387 DNA fragments (bands) were amplified from the genomic DNA of 92 individuals belonging to twenty-one populations of C . spinosa , of which 378 (97.7%) were polymorphic. High level of genetic diversity (percentage of polymorphic loci = 98...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692554/seed-protein-fraction-electrophoresis-in-peanut-arachis-hypogaea-l-accessions-and-wild-species
#5
Apekshita Singh, Soom Nath Raina, Vijay Rani Rajpal, Anurudh K Singh
Total seed storage proteins were studied in 50 accessions of A. hypogaea (11 A. hypogaea ssp. hypogaea var hypogaea , 13 A. hypogaea ssp. hypogaea var hirsuta , 11 A. hypogaea ssp. fastigiata var fastigiata and 15 A. hypogaea ssp. fastigiata var. vulgaris accessions) in SDS PAGE. These accessions were also analysed for albumin and globulin seed protein fractions. Among the six seed protein markers presently used, it was found that globulin fraction showed maximum diversity (77.2%) in A. hypogaea accessions followed by albumin (52...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692553/validation-of-reference-genes-for-accurate-normalization-of-gene-expression-with-quantitative-real-time-pcr-in-haloxylon-ammodendron-under-different-abiotic-stresses
#6
Bo Wang, Huihui Du, Zhengpei Yao, Cai Ren, Li Ma, Jiao Wang, Hua Zhang, Hao Ma
Haloxylon ammodendron plays an important role in maintaining the structure and function of the entire ecosystem where it grows. No suitable reference genes have been reported in H. ammodendron plants to date. In this study, a total of 8 reference genes ( 18S , ACT1 , ACT7 , UBC18 , TUA5 , GAPDH , EF - 1α and UBQ10 ) were selected from the available trancriptome database, and the expression stability of these 8 candidate genes was validated under different abiotic stress with three different statistical algorithms (geNorm, NormFinder and BestKeeper)...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692552/elucidating-the-role-of-osmotic-ionic-and-major-salt-responsive-transcript-components-towards-salinity-tolerance-in-contrasting-chickpea-cicer-arietinum-l-genotypes
#7
Jogendra Singh, Vijayata Singh, P C Sharma
The growth of chickpea ( Cicer arietinum L.) is extremely hampered by salt stress. Understanding of physio-biochemical and molecular attributes along with morphological traits contributing to the salinity tolerance is important for developing salt tolerant chickpea varieties. To explore these facts, two genotypes CSG8962 and HC5 with contrasting salt tolerance were evaluated in the salinity stress (Control and 120 mM NaCl) conditions. CSG8962 maintained lower Na/K ratio in root and shoot, trammeled Na translocation to the shoots from roots compared to HC5 which ascribed to better exclusion of salt from its roots and compartmentation in the shoot...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692551/foliar-application-of-ascorbic-and-citric-acids-enhanced-red-spur-apple-fruit-quality-bioactive-compounds-and-antioxidant-activity
#8
Afsaneh Allahveran, Alireza Farokhzad, Mohammadreza Asghari, Ali Sarkhosh
This study aimed to assess the effect of the foliar application of ascorbic acid (AA) and citric acid (CA) on total antioxidant activity (TAA), total phenolics, total flavonoids, total anthocyanin content, antioxidant enzymes, phenylalanine ammonialyase (PAL), and polyphenol oxidase (PPO) activities in apple 'Red Spur'. The experiment was conducted on 12-years-old trees 'Red Spur' grafted on MM106 rootstock. The trees were sprayed with AA (0, 200 and 400 mg L-1 ) and/or CA (0, 200 and 400 mg L-1 ) at three different times during summer...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692550/effect-of-soil-application-of-humic-acid-on-nutrients-uptake-essential-oil-and-chemical-compositions-of-garden-thyme-thymus-vulgaris-l-under-greenhouse-conditions
#9
Alireza Noroozisharaf, Maryam Kaviani
Humic acid is natural biological organic, which has a high effect on plant growth and quality. However, the mechanisms of the promoting effect of humic acid on the volatile composition were rarely reported. In this study, the effects of soil application of humic acid on the chemical composition and nutrients uptake of Thymus vulgaris were investigated. Treatments comprised 0, 50, 75 and 100 g m-2 . Essential oil was extracted by hydrodistillation and analyzed using GC-MS and GC-FID. Essential oil content was enhanced by increase of the humic acid level and its content ranged from 0...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692549/a-novel-trichoderma-fusant-for-enhancing-nutritional-value-and-defence-activity-in-chickpea
#10
Aradhana Mishra, Chandra Shekhar Nautiyal
In recent years, due to the rise in food consumption, much of the attention has been focused to increase the yield of the agricultural crops which resulted in compromised nutritional quality. Efforts have to be undertaken to enhance the nutritional attributes of legumes, cereals and staple food crops by increasing amino acids and mineral content. In the present study, we evaluated a protoplast fusant ( H. lixii MTCC 5659) for its ability to enhance nutritional value and defence activity in chickpea. Essential amino acids; methionine (9...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692548/extraction-profiling-and-bioactivity-analysis-of-volatile-glucosinolates-present-in-oil-extract-of-brassica-juncea-var-raya
#11
Priyanka Bassan, Sakshi Bhushan, Tajinder Kaur, Rohit Arora, Saroj Arora, Adarsh Pal Vig
Cruciferous vegetables are rich source of glucosinolates (GSLs), which in presence of myrosinase enzyme cause hydrolytic cleavage and result in different hydrolytic products like isothiocyanates, thiocyanates, nitriles and epinitriles. The GSLs hydrolytic products are volatile compounds, which are known to exhibit bioactivities like antioxidant, fungicidal, bioherbicidal and anticancer. Among the Brassicaceae family, Brassica juncea is very well known for high content of GSLs. In the present study, the isolation of volatile oil of B...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692547/characterization-of-chickpea-cicer-arietinum-l-lectin-for-biological-activity
#12
Ajay Kumar Gautam, Neha Gupta, Dakshita T Narvekar, Rajni Bhadkariya, Sameer S Bhagyawant
Lectins are proteins that are subject of intense investigations. Information on lectin from chickpea ( Cicer arietinum L.) with respect to its biological activities are very limited. In this study, we purified lectin from the seeds of chickpea employing DEAE-cellulose and SP-Sephadex ion exchange chromatography and identified its molecular subunit mass as 35 kDa. The free radical scavenging activity of lectin measured by the DPPH assay has IC50 of 0.88 µg/mL. Lectin exerted antifungal activity against Candida krusei , Fusarium oxysporium oxysporium , Saccharomyces cerevisiae and Candida albicans , while antibacterial activity against E...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692546/proteomics-assisted-profiling-of-antimicrobial-peptide-signatures-from-black-pepper-piper-nigrum-l
#13
P Umadevi, M Soumya, Johnson K George, M Anandaraj
Plant antimicrobial peptides are the interesting source of studies in defense response as they are essential components of innate immunity which exert rapid defense response. In spite of abundant reports on the isolation of antimicrobial peptides (AMPs) from many sources, the profile of AMPs expressed/identified from single crop species under certain stress/physiological condition is still unknown. This work describes the AMP signature profile of black pepper and their expression upon Phytophthora infection using label-free quantitative proteomics strategy...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692545/selection-and-validation-of-reference-genes-for-quantitative-gene-expression-analyses-in-various-tissues-and-seeds-at-different-developmental-stages-in-bixa-orellana-l
#14
Viviane S Moreira, Virgínia L F Soares, Raner J S Silva, Aurizangela O Sousa, Wagner C Otoni, Marcio G C Costa
Bixa orellana L., popularly known as annatto, produces several secondary metabolites of pharmaceutical and industrial interest, including bixin, whose molecular basis of biosynthesis remain to be determined. Gene expression analysis by quantitative real-time PCR (qPCR) is an important tool to advance such knowledge. However, correct interpretation of qPCR data requires the use of suitable reference genes in order to reduce experimental variations. In the present study, we have selected four different candidates for reference genes in B...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692544/transcriptomic-analysis-of-differentially-expressed-genes-in-flower-buds-of-genetic-male-sterile-and-wild-type-cucumber-by-rna-sequencing
#15
Yike Han, Xianyun Wang, Fengyue Zhao, Shang Gao, Aimin Wei, Zhengwu Chen, Nan Liu, Zhenxian Zhang, Shengli Du
Cucumber ( Cucumis sativus L. ) pollen development involves a diverse range of gene interactions between sporophytic and gametophytic tissues. Previous studies in our laboratory showed that male sterility was controlled by a single recessive nuclear gene, and occurred in pollen mother cell meiophase. To fully explore the global gene expression and identify genes related to male sterility, a RNA-seq analysis was adopted in this study. Young male flower-buds (1-2 mm in length) from genetic male sterility (GMS) mutant and homozygous fertile cucumber (WT) were collected for two sequencing libraries...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692543/transcriptome-analysis-of-hevea-brasiliensis-in-response-to-exogenous-methyl-jasmonate-provides-novel-insights-into-regulation-of-jasmonate-elicited-rubber-biosynthesis
#16
Jin-Ping Liu, Jin Hu, Yan-Hui Liu, Cui-Ping Yang, Yu-Fen Zhuang, Xiu-Li Guo, Yi-Jian Li, Liangsheng Zhang
The phytohomorne methyl jasmonate (MeJA) is known to trigger extensive reprogramming of gene expression leading to transcriptional activation of many secondary metabolic pathways. However, natural rubber is a commercially important secondary metabolite and little is known about the genetic and genomic basis of jasmonate-elicited rubber biosynthesis in rubber tree ( Hevea brasiliensis ). RNA sequencing (RNA-seq) of H. brasiliensis bark treated with 1 g lanolin paste containing 0.02% w/w MeJA for 24 h (M2) and 0...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29515328/seed-pretreatment-with-magnetic-field-alters-the-storage-proteins-and-lipid-profiles-in-harvested-soybean-seeds
#17
Ramalingam Radhakrishnan
The increase in crop productivity is an urgent need of the time to reduce scarcity of food in underdeveloped countries. Several biological, chemical and physical methods have been applied to promote crop yield. Application of magnetic field (MF) is an emerging physical method used to increase plant growth and yield. The reports on MF pretreatment-induced nutritional changes in harvested seeds are scarce. We previously identified the optimal frequency of MF to improve plant growth and yield as 1500 nT at 10...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29515327/antioxidant-potential-in-stevia-rebaudiana-callus-in-response-to-polyethylene-glycol-paclobutrazol-and-gibberellin-treatments
#18
Shokoofeh Hajihashemi, Shakiba Rajabpoor, Ivica Djalovic
The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l-1 ) and gibberellin (GA; 0 and 2 mg l-1 ) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2 O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29515326/identification-and-characterization-of-long-noncoding-rna-in-paulownia-tomentosa-treated-with-methyl-methane-sulfonate
#19
Zhe Wang, Bingbing Li, Yongsheng Li, Xiaoqiao Zhai, Yanpeng Dong, Minjie Deng, Zhenli Zhao, Yabing Cao, Guoqiang Fan
Paulownia is a tree native to China, with important ecological and economic value. Long noncoding RNAs (lncRNAs) are known to play important roles in eukaryotic gene regulation. However, no lncRNAs have been reported in Paulownia so far. We performed RNA sequencing of two Paulownia tomentosa lncRNA libraries constructed from the terminal buds of normal untreated seedlings and 60 mg L-1 MMS-treated seedlings, and obtained a total of 2531 putative lncRNAs. The average length of the lncRNA transcripts was much less than the average length of the mRNA transcripts in the P...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29515325/riboswitch-regulation-in-cyanobacteria-is-independent-of-their-habitat-adaptations
#20
Payal Singh, Nilesh Kumar, Minesh Jethva, Saurabh Yadav, Pragati Kumari, Archana Thakur, Hemant Ritturaj Kushwaha
Cyanobacteria are one of the ancient bacterial species occupying a variety of habitats with diverse metabolic preferences. RNA regulators like riboswitches play significant role in controlling the gene expression in prokaryotes. The taxonomic distribution of riboswitches suggests that they might be one of the oldest mechanisms of gene control system. In this paper, we analyzed the distribution of different riboswitch families in various cyanobacterial genomes. It was observed that only four riboswitch classes were abundant in cyanobacteria, B12 -element (Cob)/AdoCbl/AdoCbl-variant riboswitch being the most abundant...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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