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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/28878510/antimicrobial-activity-of-essential-oil-and-aqueous-and-ethanol-extracts-of-teucrium-polium-l-subsp-gabesianum-l-h-from-tunisia
#1
Mahmoud Ben Othman, Karima Bel Hadj Salah-Fatnassi, Saida Ncibi, Amer Elaissi, Lazhar Zourgui
The antimicrobial effects of essential oil, ethanol and aqueous extracts of Teucrium polium L. were investigated against 13 microorganisms. Extracts and essential oil were obtained from maceration, decoction and hydrodistillation respectively. Samples were tested for their antimicrobial activity using the disk diffusion, the agar dilution and the agar incorporation method. Essential oil was analysed using GC/MS, results showed that β-pinene (35.97%) and α-pinene (13.32%) were the main components. Furthermore, essential oil exhibited the highest antimicrobial activity, it was most effective against Proteus mirabilis, Staphylococcus aureus and Citrobacter freundei where inhibition zone ranged between 15 and 25 mm, and with the microbial inhibitory concentration (MIC) values of 0...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878509/an-improved-micropropagation-system-ex-vitro-rooting-and-validation-of-genetic-homogeneity-in-wild-female-momordica-dioica-an-underutilized-nutraceutical-vegetable-crop
#2
Sumitra Kumari Choudhary, Ashok Kumar Patel, Harish, Smita Shekhawat, Narpat S Shekhawat
Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA)...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878508/assessment-of-sucrose-transporters-metabolites-and-sucrose-phosphate-synthase-in-different-sugarcane-tissues
#3
Abdelaleim Ismail ElSayed, Axel Lehrer, Mohsen Ebrahim, Azza H Mohamed, Ewald Komor
The expression of sucrose-phosphate synthase II (SPSII) and sucrose transporters ShSUT1A and ShSUT4 were determined by RT-PCR and qRT-PCR in the sink and source leaves and in rind and pith of mature internodes of four high-yielding Hawaiian sugarcane cultivars. Expression of SPSII, ShSUT1A, and ShSUT4 was lower in pith than in rind, except in one cultivar, but else quite similar in the cultivars. The strong expression of transporter ShSUT4 in the rind of the internodes may hint to a special role of ShSUT4 in the rind...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878507/improvement-of-yield-and-quality-of-two-spinacia-oleracea-l-varieties-by-using-different-fertilizing-approaches
#4
Ola Alessa, Safaa Najla, Ramzi Murshed
The effects of different fertilizers [the control with no fertilizer (C), inorganic fertilization (I), combined inorganic and organic fertilizer (IOHumax1) and (IOHumax2)] on yield and nutrients contents of two spinach varieties ("Balady and Virofly") were investigated. Significant effects of variety were observed on vegetative growth and nutrients contents. While Virofly had significantly higher leaf area (236.96 cm(2)), stem diameter (7.43 mm) and fresh weight of vegetative and radical parts (15.05 and 0...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878506/analysis-of-tandem-repeat-units-of-the-promoter-of-capsanthin-capsorubin-synthase-ccs-gene-in-pepper-fruit
#5
Shi-Lin Tian, Zheng Li, Li Li, S N M Shah, Zhen-Hui Gong
Capsanthin/capsorubin synthase (Ccs) gene is a key gene that regulates the synthesis of capsanthin and the development of red coloration in pepper fruits. There are three tandem repeat units in the promoter region of Ccs, but the potential effects of the number of repetitive units on the transcriptional regulation of Ccs has been unclear. In the present study, expression vectors carrying different numbers of repeat units of the Ccs promoter were constructed, and the transient expression of the β-glucuronidase (GUS) gene was used to detect differences in expression levels associated with the promoter fragments...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878505/analysis-of-an-intraspecific-ril-population-uncovers-genomic-segments-harbouring-multiple-qtl-for-seed-relevant-traits-in-lentil-lens-culinaris-l
#6
Rintu Jha, Abhishek Bohra, Uday Chand Jha, Maneet Rana, Rakesh Kumar Chahota, Shiv Kumar, Tilak Raj Sharma
Improving seed related traits remains key objective in lentil breeding. In recent years, genomic resources have shown great promise to accelerate crop improvement. However, limited genomic resources in lentil greatly restrict the use of genomics assisted breeding. The present investigation aims to build an intraspecific genetic linkage map and identify the QTL associated with important seed relevant traits using 94 recombinant inbreds (WA 8649090 × Precoz). A total of 288 polymorphic DNA markers including simple sequence repeat (SSR), inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) were assayed on mapping population...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878504/analysis-of-spatial-distribution-of-genetic-diversity-and-validation-of-indian-foxtail-millet-core-collection
#7
Subhash Chander, K V Bhat, Ratna Kumari, Sanjay Sen, A B Gaikwad, M V C Gowda, N Dikshit
Foxtail millet [Setaria italica (L.) P. Beauv.] is an important small millet, grown as a short duration, drought tolerant crop across the world. This crop can be grown on wide ranges of soil conditions and has an immense potential for food and fodder in rainfed and arid regions of the India. In the present study, 31 primer pairs (27 SSR and 4 EST-SSR) were used to analyse the genetic diversity in 223 core collection accessions. Analysis resulted in detection of a total of 136 alleles with an average of 4.38 alleles per locus...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878503/assessment-of-genetic-diversity-and-population-structure-of-an-endemic-moroccan-tree-argania-spinosa-l-based-in-irap-and-issr-markers-and-implications-for-conservation
#8
Ouafae Pakhrou, Leila Medraoui, Chaimaa Yatrib, Mohammed Alami, Abdelkarim Filali-Maltouf, Bouchra Belkadi
Argan Tree is well known for its precious oil extracted from its seeds particularly used for the nutritional and cosmetic benefits. Because of the high international demand, the argan tree suffers from overexploitation and its cultivation is rare. Thus, the assessment of the genetic variation of this endemic tree is critically important for designing conservation strategies. In the present study and for the first time, genetic diversity of the global natural distribution of argan tree (Argania spinosa L.) in Morocco was assessed...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878502/cytogenetic-and-molecular-evidences-revealing-genomic-changes-after-autopolyploidization-a-case-study-of-synthetic-autotetraploid-phlox-drummondii-hook
#9
Tanvir H Dar, Soom N Raina, Shailendra Goel
Polyploidy is known to be common in plants; indeed most of the world's economically important crop plants are polyploids. Recent studies revealed extensive genomic changes in synthetic polyploids after genome doubling, although most of the information available is with regards to allopolyploids and little information have been generated in autopolyploids. In the present study, we used Phlox drummondii Hooker (2n = 2x = 14) as a model plant to observe genomic changes, if any, in synthetic autopolyploids...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878501/genome-wide-identification-phylogeny-and-expression-analyses-of-scarecrow-like-scl-genes-in-millet-setaria-italica
#10
Hongyun Liu, Jiajia Qin, Hui Fan, Jinjin Cheng, Lin Li, Zheng Liu
As a member of the GRAS gene family, SCARECROW-LIKE (SCL) genes encode transcriptional regulators that are involved in plant information transmission and signal transduction. In this study, 44 SCL genes including two SCARECROW genes in millet were identified to be distributed on eight chromosomes, except chromosome 6. All the millet genes contain motifs 6-8, indicating that these motifs are conserved during the evolution. SCL genes of millet were divided into eight groups based on the phylogenetic relationship and classification of Arabidopsis SCL genes...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878500/genome-wide-identification-of-saur-genes-in-watermelon-citrullus-lanatus
#11
Na Zhang, Xing Huang, Yaning Bao, Bo Wang, Hongxia Zeng, Weishun Cheng, Mi Tang, Yuhua Li, Jian Ren, Yuhong Sun
The early auxin responsive SAUR family is an important gene family in auxin signal transduction. We here present the first report of a genome-wide identification of SAUR genes in watermelon genome. We successfully identified 65 ClaSAURs and provide a genomic framework for future study on these genes. Phylogenetic result revealed a Cucurbitaceae-specific SAUR subfamily and contribute to understanding of the evolutionary pattern of SAUR genes in plants. Quantitative RT-PCR analysis demonstrates the existed expression of 11 randomly selected SAUR genes in watermelon tissues...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878499/comparative-proteomic-analysis-of-autotetraploid-and-diploid-paulownia-tomentosa-reveals-proteins-associated-with-superior-photosynthetic-characteristics-and-stress-adaptability-in-autotetraploid-paulownia
#12
Lijun Yan, Guoqiang Fan, Minjie Deng, Zhenli Zhao, Yanpeng Dong, Yongsheng Li
To enlarge the germplasm resource of Paulownia plants, we used colchicine to induce autotetraploid Paulownia tomentosa, as reported previously. Compared with its diploid progenitor, autotetraploid P. tomentosa exhibits better photosynthetic characteristics and higher stress resistance. However, the underlying mechanism for its predominant characteristics has not been determined at the proteome level. In this study, isobaric tag for relative and absolute quantitation coupled with liquid chromatography-tandem mass spectrometry was employed to compare proteomic changes between autotetraploid and diploid P...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878498/improved-tolerance-against-helicoverpa-armigera-in-transgenic-tomato-over-expressing-multi-domain-proteinase-inhibitor-gene-from-capsicum-annuum
#13
Rahul S Tanpure, Ranjit S Barbole, Vishal V Dawkar, Yashashree A Waichal, Rakesh S Joshi, Ashok P Giri, Vidya S Gupta
Plant proteinase inhibitors (PIs) are plant defense proteins and considered as potential candidates for engineering plant resistances against herbivores. Capsicum annuum proteinase inhibitor (CanPI7) is a multi-domain potato type II inhibitor (Pin-II) containing four inhibitory repeat domains (IRD), which target major classes of digestive enzymes in the gut of Helicoverpa armigera larvae. Stable integration and expression of the transgene in T1 transgenic generation, were confirmed by established molecular techniques...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878497/biochemical-basis-of-improvement-of-defense-in-tomato-plant-against-fusarium-wilt-by-cacl2
#14
Nilanjan Chakraborty, Swarnendu Chandra, Krishnendu Acharya
The objective of this study was to investigate the effectiveness of calcium chloride (CaCl2), as potential elicitor, on tomato plants against Fusarium oxysporum f. sp. lycopersici. Foliar application of CaCl2 showed significant reduction of wilt incidence after challenge inoculation. Increased production of defense and antioxidant enzymes was observed in elicitor treated sets over control. Simultaneously, altered amount of phenolic acids were analyzed spectrophotometrically and by using high performance liquid chromatography...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878496/plant-growth-promoting-effect-of-bacillus-amyloliquefaciens-h-2-5-on-crop-plants-and-influence-on-physiological-changes-in-soybean-under-soil-salinity
#15
Min-Ji Kim, Ramalingam Radhakrishnan, Sang-Mo Kang, Young-Hyun You, Eun-Ju Jeong, Jong-Guk Kim, In-Jung Lee
This study was aimed to identify plant growth-promoting bacterial isolates from soil samples and to investigate their ability to improve plant growth and salt tolerance by analysing phytohormones production and phosphate solubilisation. Among the four tested bacterial isolates (I-2-1, H-1-4, H-2-3, and H-2-5), H-2-5 was able to enhance the growth of Chinese cabbage, radish, tomato, and mustard plants. The isolated bacterium H-2-5 was identified as Bacillus amyloliquefaciens H-2-5 based on 16S rDNA sequence and phylogenetic analysis...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878495/the-expression-of-a-candidate-cucumber-fruit-sugar-starvation-marker-gene-cssef1-is-enhanced-in-malformed-fruit-induced-by-salinity
#16
Akio Tazuke, Tsuguki Kinoshita, Munehiko Asayama
The cucumber (Cucumis sativus L.) gene Cucumis sativus Somatic Embryogenesis Zinc Finger 1 (CsSEF1) was suggested to be a good marker gene for sugar starvation in fruit. The expression of this gene in fruits is dramatically upregulated in plants that have suffered either complete defoliation or prolonged darkness. CsSEF1 was initially discovered as a gene that was upregulated during somatic embryogenesis. We examined the difference in fruit parts and the effect of pollination on the upregulation of CsSEF1 induced by defoliation treatment...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878494/responses-of-wheat-plants-to-interactions-of-24-epibrassinolide-and-glomus-mosseae-in-saline-condition
#17
Cobra Tofighi, Ramazan Ali Khavari-Nejad, Farzaneh Najafi, Khadijeh Razavi, Farhad Rejali
This study was designed to investigate the possible effects of 24-Epibrassinolide (BR), arbuscular mycorrhizal (AM) fungus, Glomus mosseae, singularly and collectively under salt stress in wheat (Triticum aestivum L.) plants. After foliar spraying of mycorrhizal and non-mycorrhizal plants by 5 µM epibrassinolide (24-Epi), they were treated with 0 and 150 mM NaCl for 2 weeks and then harvested. The results showed interactions of G. mosseae and 24-Epi could alleviate the adverse effects of salinity by improving relative water content (RWC) of leaves (62%), relative growth rate (40...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878493/improvement-of-drought-tolerance-of-soybean-plants-by-using-methyl-jasmonate
#18
Heba Ibrahim Mohamed, Hanan Helmy Latif
Methyl jasmonate (MeJA) is a naturally occurring plant growth regulator and play vital roles in plant defense and many developmental processes such as root growth and seed germination. This study was undertaken to study the possible role of using methyl jasmonate to alleviate the adverse effect of water stress on soybean genotypes (Giza 22 and 35). The results showed that water stress reduced shoot length, fresh and dry weights of shoot and root, photosynthetic pigments, relative water content and oil content in the shoots of all soybean genotypes...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878492/biochemical-physiological-and-molecular-evaluation-of-rice-cultivars-differing-in-salt-tolerance-at-the-seedling-stage
#19
Mojtaba Kordrostami, Babak Rabiei, Hassan Hassani Kumleh
Changes in the antioxidant enzymes, lipid peroxidation, sodium and potassium, chlorophyll, H2O2 and proline content were monitored in the leaves of 42 rice varieties which were not yet well-documented for the salinity tolerance under different salinity levels. The tolerant varieties (FL478, Hassani, Shahpasand, Gharib and Nemat) showed signs of tolerance (lower Na(+)/K(+) ratio, high proline accumulation, less membrane damage, lower H2O2 production, and higher superoxide dismutase and catalase activity) very well...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28878491/salt-stress-reveals-differential-physiological-biochemical-and-molecular-responses-in-t-monococcum-and-t-durum-wheat-genotypes
#20
REVIEW
Sana Tounsi, Kaouthar Feki, Dorsaf Hmidi, Khaled Masmoudi, Faiçal Brini
Salt stress responses implicate a complex mechanism and differ from plant species to another. In this study, we analyzed the physiological, biochemical and molecular responses to salt stress of the diploid wheat (T. monococcum) and compared to the tetraploid wheat (T. durum). Our results showed that the diploid wheat cultivar (cv. Turkey) is relatively tolerant to different salt stress conditions than the tetraploid wheat cultivar (cv. Om Rabia3). This tolerance was manifested by significant germination, plant growth and uptake of water generating cell turgor and development...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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