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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/29158643/in-vitro-propagation-ex-vitro-rooting-and-leaf-micromorphology-of-bauhinia-racemosa-lam-a-leguminous-tree-with-medicinal-values
#1
Udit Sharma, Vinod Kataria, N S Shekhawat
A micropropagation system for Bauhinia racemosa Lam. was developed involving axillary shoot proliferation and ex vitro rooting using nodal explants obtained from mature tree. MS medium with 3.0 mg l(-1) BA (6-benzyladenine) was optimum for shoot bud induction. For shoot multiplication, mother explants were transferred repeatedly on medium containing low concentration of BA (0.75 mg l(-1)). Number of shoots was increased up to two passages and decreased thereafter. Shoot multiplication was further enhanced on MS medium containing 0...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158642/insights-into-nuclear-dna-content-hydrogen-peroxide-and-antioxidative-enzyme-activities-during-transverse-thin-cell-layer-organogenesis-and-ex-vitro-acclimatization-of-malaxis-wallichii-a-threatened-medicinal-orchid
#2
Biswajit Bose, Suman Kumaria, Hiranjit Choudhury, Pramod Tandon
Malaxis wallichii (Lindl.) Deb, a small, perennial, monopodial, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000 m asl. The pseudobulbs are important ingredients of century old drug 'Ashtavarga' and a polyherbal energetic tonic 'Chyavanprash'. An efficient genetically stable in vitro propagation protocol using transverse thin cell layer culture system was established for M. wallichii. In the present report, meta-topolin alone proved to be three times more beneficial compared to other routinely used cytokinins in inducing highest number of shoot buds, plant height and growth of regenerated shoots...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158641/development-of-an-efficient-and-reproducible-regeneration-system-in-wheat-triticum-aestivum-l
#3
Rakesh Kumar, Harohalli Masthigowda Mamrutha, Amandeep Kaur, Karnam Venkatesh, Anita Grewal, Raj Kumar, Vinod Tiwari
The availability of reproducible regeneration system through tissue culture is a major bottleneck in wheat improvement program. The present study has considered to develop an efficient callus induction and regeneration system using mature and immature embryos as explants in recently released agronomically superior spring wheat varieties. An efficient sterilization process was standardized using 0.1% HgCl2 and 70% ethanol for both seeds and embryos. The maximum possible combinations of plant growth regulators (PGRs) were evaluated for their effect on different wheat regeneration processes through tissue culture starting from callus to root induction...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158640/a-sucrose-non-fermenting-1-related-protein-kinase-1-gene-from-potato-stsnrk1-regulates-carbohydrate-metabolism-in-transgenic-tobacco
#4
Feibing Wang, Yuxiu Ye, Xinhong Chen, Jizhong Wang, Zhiyuan Chen, Qing Zhou
Sucrose non-fermenting-1-related protein kinase 1 (SnRK1) has been shown to play an essential role in regulating saccharide metabolism and starch biosynthesis of plant. The regulatory role of StSnRK1 from potato in regulating carbohydrate metabolism and starch accumulation has not been investigated. In this work, a cDNA encoding the SnRK1 protein, named StSnRK1, was isolated from potato. The open reading frame contained 1545 nucleotides encoding 514 amino acids. Subcellular localization analysis in onion epidermal cells indicated that StSnRK1 protein was localized to the nucleus...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158639/mining-and-characterization-of-est-ssr-markers-for-zingiber-officinale-roscoe-with-transferability-to-other-species-of-zingiberaceae
#5
Praveen Awasthi, Ashish Singh, Gulfam Sheikh, Vidushi Mahajan, Ajai Prakash Gupta, Suphla Gupta, Yashbir S Bedi, Sumit G Gandhi
Zingiber officinale is a model spice herb, well known for its medicinal value. It is primarily a vegetatively propagated commercial crop. However, considerable diversity in its morphology, fiber content and chemoprofiles has been reported. The present study explores the utility of EST-derived markers in studying genetic diversity in different accessions of Z. officinale and their cross transferability within the Zingiberaceae family. A total of 38,115 ESTs sequences were assembled to generate 7850 contigs and 10,762 singletons...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158638/genetic-characterization-of-chayote-sechium-edule-jacq-swartz-landraces-of-north-eastern-hills-of-india-and-conservation-measure
#6
Veerendra Kumar Verma, Avinash Pandey, Anjani Kumar Jha, S V Ngachan
Chayote or chow-chow is an underutilized cucurbit vegetable crop, widely cultivated by farmers in the backyards and Jhum lands for its tender leaves, fruits and tuberous root. In order to initiate crop improvement program in this crop, the present study was undertaken to assess the genetic variations in the 74 chow-chow landraces collected from the North Eastern Hill region of India. Wide variations for fruit colors, fruit length (6.5-21.5 cm), fruit width (4.2-10.7 cm), fruit weight (60-560 g), vitamin-C (2...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158637/marker-assisted-identification-of-restorer-gene-s-in-iso-cytoplasmic-restorer-lines-of-wa-cytoplasm-in-rice-and-assessment-of-their-fertility-restoration-potential-across-environments
#7
Amit Kumar, Prolay Kumar Bhowmick, Vikram Jeet Singh, Manoj Malik, Ashish Kumar Gupta, R Seth, M Nagarajan, S Gopala Krishnan, Ashok Kumar Singh
Iso-cytoplasmic restorers possess the same male sterile cytoplasm as the cytoplasmic male sterile (CMS) lines, thereby minimizing the potential cyto-nuclear conflict in the hybrids. Restoration of fertility of the wild abortive CMS is governed by two major genes namely, Rf3 and Rf4. Therefore, assessing the allelic status of these restorer genes in the iso-cytoplasmic restorers using molecular markers will not only help in estimating the efficiency of these genes either alone or in combination, in fertility restoration in the hybrids in different environments, but will also be useful in determining the efficacy of these markers...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158636/chromosome-segment-detection-for-seed-size-and-shape-traits-using-an-improved-population-of-wild-soybean-chromosome-segment-substitution-lines
#8
Hongyan Yang, Wubin Wang, Qingyuan He, Shihua Xiang, Dong Tian, Tuanjie Zhao, Junyi Gai
Size and shape of soybean seeds are closely related to seed yield and market value. Annual wild soybeans have the potential to improve cultivated soybeans, but their inferior seed characteristics should be excluded. To detect quantitative trait loci (QTLs)/segments of seed size and shape traits in annual wild soybean, its chromosome segment substitution lines (CSSLs) derived from NN1138-2 (recurrent parent, Glycine max) and N24852 (donor parent, Glycine soja) and then modified 2 iterations (coded SojaCSSLP3) were improved further to contain more lines (diagonal segments) and less heterozygous and missing portions...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158635/gene-expression-and-activity-of-antioxidant-enzymes-in-rice-plants-cv-brs-ag-under-saline-stress
#9
Tatiana Rossatto, Marcelo Nogueira do Amaral, Letícia Carvalho Benitez, Isabel Lopes Vighi, Eugenia Jacira Bolacel Braga, Ariano Martins de Magalhães Júnior, Mara Andrade Colares Maia, Luciano da Silva Pinto
The rice cultivar (Oryza sativa L.) BRS AG, developed by Embrapa Clima Temperado, is the first cultivar designed for purposes other than human consumption. It may be used in ethanol production and animal feed. Different abiotic stresses negatively affect plant growth. Soil salinity is responsible for a serious reduction in productivity. Therefore, the objective of this study was to evaluate the gene expression and the activity of antioxidant enzymes (SOD, CAT, APX and GR) and identify their functions in controlling ROS levels in rice plants, cultivar BRS AG, after a saline stress period...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158634/aluminum-exclusion-from-root-zone-and-maintenance-of-nutrient-uptake-are-principal-mechanisms-of-al-tolerance-in-pisum-sativum-l
#10
Natalia E Kichigina, Jan V Puhalsky, Aleksander I Shaposhnikov, Tatiana S Azarova, Natalia M Makarova, Svyatoslav I Loskutov, Vera I Safronova, Igor A Tikhonovich, Margarita A Vishnyakova, Elena V Semenova, Irina A Kosareva, Andrey A Belimov
Our study aimed to evaluate intraspecific variability of pea (Pisum sativum L.) in Al tolerance and to reveal mechanisms underlying genotypic differences in this trait. At the first stage, 106 pea genotypes were screened for Al tolerance using root re-elongation assay based on staining with eriochrome cyanine R. The root re-elongation zone varied from 0.5 mm to 14 mm and relationships between Al tolerance and provenance or phenotypic traits of genotypes were found. Tolerance index (TI), calculated as a biomass ratio of Al-treated and non-treated contrasting genotypes grown in hydroponics for 10 days, varied from 30% to 92% for roots and from 38% to 90% for shoots...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158633/comprehensive-physiological-analyses-and-reactive-oxygen-species-profiling-in-drought-tolerant-rice-genotypes-under-salinity-stress
#11
Sahana Basu, Ranjan Kumar Giri, Ibtesham Benazir, Santosh Kumar, Ravi Rajwanshi, Sharad Kumar Dwivedi, Gautam Kumar
Rice being a staple cereal is extremely susceptible towards abiotic stresses. Drought and salinity are two vital factors limiting rice cultivation in Eastern Indo-Gangetic Plains (EIGP). Present study has intended to evaluate the consequences of salinity stress on selected drought tolerant rice genotypes at the most susceptible seedling stage with an aim to identify the potential multi-stress (drought and salt) tolerant rice genotype of this region. Genotypic variation was obvious in all traits related to drought and salt susceptibility...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158632/impact-of-postharvest-exogenous-%C3%AE-aminobutyric-acid-treatment-on-cucumber-fruit-in-response-to-chilling-tolerance
#12
Parviz Malekzadeh, Fariba Khosravi-Nejad, Ali Asghar Hatamnia, Reza Sheikhakbari Mehr
Low-temperature storage is generally used to extend postharvest lifetime and to inhibit decay of cucumber fruit, but it also enhances the intensity of chilling injury. The capability of γ-aminobutyric acid to enhance antioxidant enzyme activities and reduce chilling injury was studied in cucumber (Cucumis sativus L.) fruit stored at 1 °C for 5 weeks. The purpose of this study was to define if the GABA-induced modification in antioxidant system and phospholipase activity is linked to the reduced chilling injury in cold-stored cucumber fruit...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158631/mapping-quantitative-trait-loci-for-heat-tolerance-at-the-booting-stage-using-chromosomal-segment-substitution-lines-in-rice
#13
Shan Zhu, Renliang Huang, Hnin Pwint Wai, Hongliang Xiong, Xianhua Shen, Haohua He, Song Yan
High temperature stress is a major obstacle in rice productivity. Considerable progress has been made on studying heat tolerance (HT) at different stages. However, the genetic basis of HT at the booting stage is poorly understood. In this study, we analyzed the morphological features of a heat-sensitive japonica cultivar Sasanishiki under natural high temperature stress at the booting stage. The anthers became smaller and the number, and fertility, of pollen grains were decreased significantly. As a result, there was a dramatic reduction in spikelet fertility...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158630/consequences-of-copper-treatment-on-pigeon-pea-photosynthesis-osmolytes-and-antioxidants-defense
#14
Poonam Sharma, Geetika Sirhindi, Anil Kumar Singh, Harpreet Kaur, Ruqia Mushtaq
An attempt was made to explore the effect of copper sulphate treatment on growth, photosynthesis, osmolytes and antioxidants in 15 days old seedlings of C. cajan (Pigeonpea). C. cajan seedlings were grown in 0, 1, 5 and 10 mM concentrations of copper sulphate in petriplates lined with Whatman filter paper for 15 days. Root length and shoot length was decreased in a dose dependent manner with highest decrease of 82.80 and 45.92% in 10 mM Cu stress. Photosynthetic efficiency (qP, qN and Y) was decreased in a dose dependent manner whereas NPQ was increased in 1 and 5 mM and decreased in 10 mM Cu...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158629/overexpression-of-lhsornpr1-a-npr1-like-gene-from-the-oriental-hybrid-lily-sorbonne-conferred-enhanced-resistance-to-pseudomonas-syringae-pv-tomato-dc3000-in-arabidopsis
#15
Le Wang, Zhihong Guo, Yubao Zhang, Yajun Wang, Guo Yang, Liu Yang, Li Wang, Ruoyu Wang, Zhongkui Xie
The non-expressor of the pathogenesis-related genes 1 (NPR1) is a master regulator in defense signaling of plants and plays a key role in basal and systemic acquired resistance. In this study, we isolated a NPR1-like gene from the oriental hybrid lily 'Sorbonne' (designated as LhSorNPR1) using rapid amplification of cDNA ends (RACE). The open reading frame of LhSorNPR1 consisted of 1854 bp, encoding a protein of 617 amino acids. Multiple sequence alignment revealed that LhSorNPR1 shares high similarity to NPR1-like proteins and characteristics of the BTB/POZ domain and ankyrin repeats...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158628/identification-and-expression-profiling-analysis-of-tcp-family-genes-involved-in-growth-and-development-in-maize
#16
Wenbo Chai, Pengfei Jiang, Guoyu Huang, Haiyang Jiang, Xiaoyu Li
The TCP family is a group of plant-specific transcription factors. TCP genes encode proteins harboring bHLH structure, which is implicated in DNA binding and protein-protein interactions and known as the TCP domain. TCP genes play important roles in plant development and have been evolutionarily and functionally elaborated in various plants, however, no overall phylogenetic analysis or expression profiling of TCP genes in Zea mays has been reported. In the present study, a systematic analysis of molecular evolution and functional prediction of TCP family genes in maize (Z...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158627/development-of-transcriptome-based-web-genomic-resources-of-yellow-mosaic-disease-in-vigna-mungo
#17
Rahul Singh Jasrotia, Mir Asif Iquebal, Pramod Kumar Yadav, Neeraj Kumar, Sarika Jaiswal, U B Angadi, Anil Rai, Dinesh Kumar
Vigna mungo (Urdbean) is cultivated in the tropical and sub-tropical continental region of Asia. It is not only important source of dietary protein and nutritional elements, but also of immense value to human health due to medicinal properties. Yellow mosaic disease caused by Mungbean Yellow Mosaic India Virus is known to incur huge loss to crop, adversely affecting crop yield. Contrasting genotypes are ideal source for knowledge discovery of plant defence mechanism and associated candidate genes for varietal improvement...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158626/genome-scanning-for-identification-and-mapping-of-receptor-like-kinase-rlk-gene-superfamily-in-solanum-tuberosum
#18
Sara Dezhsetan
Receptor-like kinases (RLKs) are a key class of genes that contribute to diverse phenomena from plant development to defense responses. The availability of completed potato genome sequences provide an excellent opportunity to identify and characterize RLK gene superfamily in this lineage. We identified 747 non-redundant RLK genes in the potato genome that were classified into 52 subfamilies, of which 58% members organized into tandem repeats. Nine of potato RLK subfamilies organized into tandem repeats. Also, six subfamilies exhibited lineage-specific expansion compared to Arabidopsis...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158625/comparative-genomic-hybridization-and-transcriptome-sequencing-reveal-that-two-genes-osi_14279-loc_os03g62620-and-osi_10794-loc_os03g14950-regulate-the-mutation-in-the-%C3%AE-rl-rice-mutant
#19
Xulong Wang, Fanhua Wang, Huiqiong Chen, Xiaoyu Liang, Yingmei Huang, Jicai Yi
We previously established the genetic locus of the rolled-leaf mutant, γ-rl, to chromosome 3. In this study, we performed a comparative genomic hybridization (CGH) analysis to identify the genes responsible for the γ-rl mutant phenotype. This was combined with RNA transcriptome sequencing (RNA-seq) to analyze differences in the mRNA expression in seeds 12 h after germination. Using the reference genome of the "indica type" rice from GenBank, we created a chip with 386,000 high density DNA probes designed to target chromosome 3...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158624/plant-growth-under-water-salt-stress-ros-production-antioxidants-and-significance-of-added-potassium-under-such-conditions
#20
Mohammad Abass Ahanger, Nisha Singh Tomar, Megha Tittal, Surendra Argal, R M Agarwal
Plants are confronted with a variety of environmenmtal stresses resulting in enhanced production of ROS. Plants require a threshold level of ROS for vital functions and any change in their concentration alters the entire physiology of plant. Delicate balance of ROS is maintained by an efficient functioning of intriguing indigenous defence system called antioxidant system comprising enzymatic and non enzymatic components. Down regulation of antioxidant system leads to ROS induced oxidative stress causing damage to important cellular structures and hence anomalies in metabolism...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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