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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/30425444/optimization-of-in-vitro-regeneration-of-haloxylon-salicornicum-a-keystone-species-of-extreme-arid-regions
#1
Deepak Krishna Vyas, Harchand R Dagla
Haloxylon salicornicum is an economically important perennial woody shrub of family Amaranthaceae and is a good source of food additives, fodder, fuel and nutrients in Indian Thar Desert. In vitro regeneration of H. salicornicum through nodal shoot segments is achieved in present investigation. Young green shoots in horizontal orientation were found to be more responsive than vertical orientation. Axillary shoot buds were activated on MS medium containing 10 µM BAP. Adventitious roots with intense root hairs were induced on horizontally placed shoots on half strength MS medium containing 2 µM NOA + 100 mg L-1 activated charcoal...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425443/exogenous-melatonin-trigger-biomass-accumulation-and-production-of-stress-enzymes-during-callogenesis-in-medicinally-important-prunella-vulgaris-l-selfheal
#2
Hina Fazal, Bilal Haider Abbasi, Nisar Ahmad, Mohammad Ali
The objective of the current study was to monitor the variations caused by the application of exogenous melatonin on growth kinetics and production of stress enzymes in Prunella vulgaris . Leaf and petiole explants were used for callogenesis. These explants were inoculated on Murashige and Skoog media containing various concentrations of melatonin alone or in combination with 2.0 mg/l naphthalene acetic acid. Herein, a maximum of 3.18-g/100 ml fresh biomass accumulation was observed on day 35 during log phase of growth kinetics at 1...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425442/physical-state-of-the-culture-medium-triggers-shift-in-morphogenetic-pattern-from-shoot-bud-formation-to-somatic-embryo-in-solanum-khasianum
#3
Shatrujeet Pandey, Pragya Shukla, Pratibha Misra
Solanum khasianum is a rich source of steroidal alkaloids that are important secondary metabolites with enormous pharmaceutical uses. Development of plantlets from somatic tissues, under in vitro conditions, takes place both through adventitious shoot bud differentiation or somatic embryogenesis (SE) pathway. We observed that the physical state of medium, solid or liquid, determined the regenerant differentiation patterns from root segment explants in S. khasianum . In the solidified medium, the root segments developed adventitious shoot buds whereas somatic embryos were regenerated in the liquid medium...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425441/in-vitro-propagation-of-some-old-persian-cypress-accessions-cupressus-sempervirens-l-by-embryo-culture
#4
Hamid Reza Karimi, Kobra Janghorban, Mahmoud Raqamy, Homayoun Farahmand
In order to study in vitro propagation of some old Persian cypress genotypes ( Cupressus sempervirens L.), embryos of twenty old cypress accessions were cultured on MS and SH media containing 100 and 200 mg L-1 myo-inositol. Germination percentage and growth parameters of produced plantlets and their hardening off were evaluated. Results showed that the highest germination percentage and germination rate was obtained with MS medium containing 100 mg L-1 myo-inositol, although no significant difference was observed with MS containing 200 mg L-1 myo-inositol and SH containing 100 mg L-1 myo-inositol...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425440/an-improved-micropropagation-and-ex-vitro-rooting-of-a-commercially-important-crop-henna-lawsonia-inermis-l
#5
P C Shiji, E A Siril
An improved micropropagation protocol has been developed for a cosmetically important, dye yielding crop, henna ( Lawsonia inermis ). Quality of henna product is governed by naphthoquinone based pigment lawsone, thus in vitro multiplication of superior healthy plant to achieve enhanced productivity in terms of dye content and biomass deserve due attention. In the present study, nodal explants collected from an elite plant screened on the basis of superiority in lawsone content was cultured on MS medium supplemented with 0...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425439/molecular-diversity-assessment-of-a-world-collection-of-safflower-genotypes-by-srap-and-scot-molecular-markers
#6
Pooran Golkar, Niloofar Mokhtari
Safflower ( Carthamus tinctorius L.) is considered as an oil crop that is rich in medicinal and industrial properties. In this study, the genetic diversity of safflower was assessed using 12 polymorphic sequence-related amplified polymorphism (SRAPs) and 11 polymorphic start codon targeted (SCoT) markers in 100 genotypes of safflower gathered from different geographical regions of the world. The 23 primers generated a total of 227 polymorphism fragments with a mean of 68.2% within the range of 3 (SCoT 31 and SCoT 35) to 13 (SCoT 35) bands per primer...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425438/validation-of-qtls-for-plant-ideotype-earliness-and-growth-habit-traits-in-pigeonpea-cajanus-cajan-millsp
#7
Prakash G Patil, Abhishek Bohra, Naik S J Satheesh, Jyotirmay Dubey, Praveen Pandey, Dibendu Dutta, Farindra Singh, I P Singh, N P Singh
Pigeonpea productivity is greatly constrained by poor plant ideotype of existing Indian cultivars. Enhancing pigeonpea yield demands a renewed focus on restructuring the ideal plant type by using more efficient approaches like genomic tools. Therefore, the present study aims to identify and validate a set of QTLs/gene(s) presumably associated with various plant ideotype traits in pigeonpea. A total of 133 pigeonpea germplasms were evaluated along with four checks in the augmented design for various ideotype traits i...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425437/mapping-qtls-for-physiological-and-biochemical-traits-related-to-grain-yield-under-control-and-terminal-heat-stress-conditions-in-bread-wheat-triticum-aestivum-l
#8
Faramarz Sohrabi Chah Hassan, Mahmood Solouki, Barat Ali Fakheri, Nafiseh Mahdi Nezhad, Bahram Masoudi
In order to detect genomic regions with different effects for some of the physiological and biochemical traits of wheat, four experiments were conducted at Research Farm of Agricultural and Natural Resources Research Center of Zabol in 2015-2016 and 2016-2017 growing seasons. The experiments were carried out using four alpha lattice designs with two replications under non-stress and terminal heat stress conditions. Plant materials used in this study included 167 recombinant inbred lines and their parents ('SeriM82' and 'Babax')...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425436/characterization-of-hawthorn-crataegus-spp-genotypes-by-ssr-markers
#9
Murat Güney, Salih Kafkas, Hakan Keles, Servet Aras, Sezai Ercişli
Hawthorn ( Crataegus spp.) is an edible wild fruit that is used in traditional medicine, landscape studies, and food and beverage industries in many countries. It is an important wild plant species in Turkey and is numerous in the Yozgat Province. Genetic and breeding studies on hawthorn are very limited. Therefore, we aimed to characterize 91 hawthorn genotypes using simple sequence repeat (SSR) markers. The SSRs were developed from apple and pear and were screened in hawthorn for amplification and polymorphisms...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425435/solvent-based-optimization-for-extraction-and-stability-of-thymoquinone-from-nigella-sativa-linn-and-its-quantification-using-rp-hplc
#10
Mohammed Shariq Iqbal, Ausaf Ahmad, Brijesh Pandey
The Nigella sativa pharmacological properties are mainly ascribed to its volatile oil, of which thymoquinone is an important bioactive component. Surprisingly, till date, no standard formulation or thymoquinone rich N. sativa extract is under clinical use probably due to its poor extraction and lesser stability in the already used solvents. In the present investigation solubility, extraction, percent composition and total antioxidant activity from the seeds of N. sativa was explored using five solvents. An HPLC method was standardized in an isocratic system (C-18 column, flow rate of 1...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425434/in-vitro-assessment-of-antimicrobial-activity-of-aqueous-and-alcoholic-extracts-of-moss-atrichum-undulatum-hedw-p-beauv
#11
Kamakshi Saxena, Uma Yadav
Bryophytes, the shade loving plants, have tremendous medicinal properties. The aqueous and alcoholic extracts of Atrichum undulatum ( Hedw. ) P. Beauv. were analysed for antimicrobial properties against the fungi Aspergillus fumigatus and Fusarium oxysporum and the bacteria Escherichia coli, Bacillus mycoides, Proteus mirabilis, Staphylococcus aureus and Salmonella typhi. The study is an attempt to investigate the medicinal properties of Atrichum undulatum ( Hedw. ) P. Beauv. using disc-diffusion method. No inhibition was observed against A...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425433/cytotoxic-effect-of-semialarium-mexicanum-miers-mennega-root-bark-extracts-and-fractions-against-breast-cancer-cells
#12
Juan Maldonado-Cubas, Eduardo San Martin-Martínez, Cinthya Nathaly Quiroz-Reyes, Rocio Guadalupe Casañas-Pimentel
The root bark of Semialarium mexicanum (Miers) Mennega (cancerina) is traditionally used in Mexico to treat cancer. However, there are no studies supporting its use. We evaluated whether S. mexicanum root bark induces cytotoxicity in breast cancer cells to determine if it has potential applications in the treatment of this disease. Extracts of S. mexicanum root bark in petroleum ether, ethanol, and water were obtained by ultrasound-assisted extraction. MTT and WST-1 assays were used to evaluate the cytotoxicity of the extracts toward breast cancer cells (MDA-MB-231 and MCF7), non-tumorigenic breast-derived cells (MCF 10A), and peripheral blood mononuclear cells (PBMCs)...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425432/biochemical-diversity-evaluation-in-chickpea-accessions-employing-mini-core-collection
#13
Sameer Suresh Bhagyawant, Ajay Kumar Gautam, Dakshita Tanaji Narvekar, Neha Gupta, Amita Bhadkaria, Nidhi Srivastava, Hari D Upadhyaya
The seeds of chickpea provide an exceptional source of dietary proteins and is one of the important legumes in both developed and developing countries over the world. The available germplasm of cultivated chickpea is deficient in desired biochemical signatures. To identify new sources of variations for breeding, reduced subsets of germplasm such as mini-core collection can be explored as an effective resource. In the present investigation, mini-core collections consisting of 215 accessions of chickpea were extensively evaluated for tapping biochemical diversity...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425431/characterization-of-backcross-introgression-lines-derived-from-oryza-nivara-accessions-for-photosynthesis-and-yield
#14
Yadavalli Venkateswara Rao, Divya Balakrishnan, Krishnam Raju Addanki, Sukumar Mesapogu, Thuraga Vishnu Kiran, Desiraju Subrahmanyam, Sarla Neelamraju, Sitapathi Rao Voleti
Improvement of photosynthetic traits is a promising strategy to break the yield potential barrier of major food crops. Leaf photosynthetic traits were evaluated in a set of high yielding Oryza sativa , cv. Swarna ×  Oryza nivara backcross introgression lines (BILs) along with recurrent parent Swarna, both in wet (Kharif) and dry (Rabi) seasons in normal irrigated field conditions. Net photosynthesis ( P N ) ranged from 15.37 to 23.25 µmol (CO2 ) m-2  s-1 in the BILs. Significant difference in P N was observed across the seasons and genotypes...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425430/comparative-analysis-of-photosynthetic-and-respiratory-parameters-in-the-psychrophilic-unicellular-green-alga-koliella-antarctica-cultured-in-indoor-and-outdoor-photo-bioreactors
#15
Vincenza Vona, Vittoria Di Martino Rigano, Carlo Andreoli, Ornella Lobosco, Marianna Caiazzo, Anna Martello, Simona Carfagna, Giovanna Salbitani, Carmelo Rigano
Effect of temperatures and illumination of temperate winter on photosynthesis and respiration was studied in the psychrophilic microalgae, Koliella antarctica (Trebouxiophyceae). Outdoor and indoor algal cultures were compared. Photosynthetic as well as respiration rates increased as light and temperature increased, until 35 °C, more in outdoor than in indoor cells, in agreement with the calculated Q 10 values. K. antarctica showed important strategy mechanisms of adaption to the several temperature and light conditions...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425429/adenine-type-and-diphenyl-urea-derived-cytokinins-improve-the-postharvest-performance-of-iris-germanica-l-cut-scapes
#16
Syed Sabhi Ahmad, Inayatullah Tahir, Arif Shafi Wani, Riyaz Ahmad Dar, Shaziya Nisar
An experiment was designed to evaluate the effect of various adenine derived cytokinins (kinetin and 6-benzylaminopurine) and diphenyl urea cytokinin (thidiazuron) on the postharvest performance of cut scapes of Iris germanica . Flower scapes were harvested with the oldest bud at '1 day before anthesis stage', brought to laboratory under water, cut to a uniform length of 35 cm, divided into three sets viz., kinetin (KIN), 6-benzyl aminopurine (BAP) and thidiazuron (TDZ). Each set of scapes was treated with a particular cytokinin alone or in combination with 0...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425428/concentration-of-some-metals-in-soil-and-plant-organs-and-their-biochemical-profiles-in-tulipa-luanica-t-kosovarica-and-t-albanica-native-plant-species
#17
Mirsade Osmani, Metin Tuna, Isa R Elezaj
The purpose of this study was to determine the concentration of some metals (Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn, Ca and Mg) in soil of serpentine and limestone sites, their bioaccumulation and impact on some biochemical parameters in T. luanica , T. kosovarica and T. albanica plants. T. kosovarica and T. albanica grows in serpentine soil, while T. luanica grow in limestone soil. The research showed that concentrations of Cd, Co, Cr, Fe, Mn and Ni were significantly higher at serpentine soil sites in comparison with limestone sites, while concentrations of Pb, Cd, Co and Cr in bulbs, leaves and seeds were under the limit of detection...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425427/silicon-influences-growth-and-mycorrhizal-responsiveness-in-strawberry-plants
#18
Roghieh Hajiboland, Narges Moradtalab, Nasser Aliasgharzad, Zarrin Eshaghi, Javad Feizy
Effect of silicon (Si) on the response of strawberry ( Fragaria  ×  ananassa var. Parus) plants to arbuscular mycorrhizal fungus (AMF) was studied under growth chamber conditions. Plants were grown in perlite irrigated with nutrient solution without (- Si) or with (+ Si) 3 mmol L-1 Si (~ 84 mg L-1 Si as Na2 SiO3 ) in the absence (- AMF) or presence (+ AMF) of fungus. Dry matter production, root colonization rate, photosynthesis rate and water relation parameters were all improved by both Si and AMF, and the highest amounts were achieved by + Si + AMF treatment...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425426/influence-of-salinity-and-fusarium-oxysporum-as-the-stress-factors-on-morpho-physiological-and-yield-attributes-in-onion
#19
Amna Shoaib, Sidra Meraj, Nafisa, Kashif Ali Khan, Muhammad Azhar Javaid
Occurrence of salt stress with the soil borne fungus Fusarium oxysporum f. sp. cepa (FOC) are potential threat to the crop yield. This investigation reports effect of the concurrent stresses (salinity and FOC) on morpho-physiological and yield attributes in onion. In vitro growth tests revealed proliferation of FOC biomass at different levels of salinity (2-8 dS m-1 ). A greenhouse pot experiment with the proposed levels of salinity (2.5, 3.5 and 4.5 dS m-1 ) in combination with FOC inoculation showed more drastic effect of combined stress on disease severity, plant growth and bulb as compared to the individual stress...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30425425/ascorbate-glutathione-cycle-confers-salt-tolerance-in-egyptian-lentil-cultivars
#20
Reda M Gaafar, Maha M Seyam
In order to investigate the salt tolerance mechanism in Egyptian lentils, five cultivars were grown under different salinity levels. The results indicated that Giza 9 is salt-tolerant, while Giza 4 is a salt-sensitive lentil cultivar, as Giza 9 showed the highest germination percentage and relative water content, the lowest decline in root and shoot length, and the highest proline content. Relative gene expression of ADH , CYS3 , APX , SOD , and GR was significantly upregulated in Giza 9 roots after 24 h of high salt treatment (250 mM), while CAT transcripts level was non-significantly reduced (0...
November 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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