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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/30042625/incidence-of-leaf-spot-disease-on-cotton-caused-by-curvularia-verruculosa-and-role-of-its-hydrolytic-enzymes-in-pathogenesis
#1
Leena P Shirsath, Sandip P Patil, Ulhas K Patil
The finding described in this study is the first report of leaf spot disease of cotton caused by Curvularia verruculosa surveyed in the state of Maharashtra (India). The isolated phytopathogenic fungal strain was identified using morphological characteristics and molecular identification of ITS gene sequence (MF784436) and D1D2 region of LSU gene (KY978073). The ability of fungal strain to secrete hydrolytic enzymes viz., pectinase, xylanase, protease, cellulase and lipase was detected. The secretion profile of hydrolytic enzymes by C...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042624/elimination-of-macro-elements-from-inoculation-and-co-cultivation-media-enhances-the-efficiency-of-agrobacterium-mediated-transformation-in-petunia
#2
Aso Nobakht Vakili, Hedayat Bagheri, Pejman Azadi
In order to evaluate the effect of inoculation and co-cultivation media elements on transformation frequency in Petunia hybrida, modified MS media with different elements were tested on Alvan and Large Flower Alvan (LF Alvan), two local cultivars. Leaf explants of both cultivars were inoculated with Agrobacterium tumefaciens strain LBA4404 (pBI121) containing neomycin phosphotransferase ( nptII ) and an intron-containing β-glucuronidase ( gus ) genes. When medium lacking KH2 PO4 , NH4 NO3 , KNO3 , and CaCl2 was used as inoculation and co-cultivation medium, a higher frequency of transformation for Alvan (22%) and LF Alvan (16%) was obtained...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042623/analysis-of-nutrient-deficiencies-affecting-in-vitro-growth-and-development-of-eucalyptus-dunnii-maiden
#3
Gustavo Pedro Javier Oberschelp, Antônio Natal Gonçalves
Although basal medium optimization is a key factor in the success of tissue culture, its mineral composition is frequently disregarded when optimizing in vitro propagation protocols. A previous work on Eucalyptus dunnii micropropagation suggests that excessive callus formation and leaf chlorosis are related to specific nutritional conditions of the basal media. Recently, a novel basal medium based on the mineral nutrient analysis of E. dunnii young stump shoots was developed and successfully tested in plant regeneration and micropropagation of E...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042622/thidiazuron-tdz-induced-organogenesis-and-clonal-fidelity-studies-in-haloxylon-persicum-bunge-ex-boiss-buhse-an-endangered-desert-tree-species
#4
Shyam Sreedhara Kurup, Fayas Thayale Purayil, Mubarak Mohammed Sultan Alkhaili, Nadia Hassan Tawfik, Abdul Jaleel Cheruth, Maher Kabshawi, Sreeramanan Subramaniam
Haloxylon persicum (Bunge ex Boiss & Buhse), is one of the hardy woody desert shrubs, which is now endangered and/or nearing extinction. Urban landscape development and overgrazing are the major threats for the erosion of this important plant species. For conserving the species, it is critical to develop an efficient in vitro regeneration protocol for rapid multiplication of large number of regenerants. Leaf explants, cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) (0...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042621/characterization-of-leaf-transcriptome-development-and-utilization-of-unigenes-derived-microsatellite-markers-in-sugarcane-saccharum-sp-hybrid
#5
Mohammad Suhail Khan, Sanjeev Kumar, Ram Kewal Singh, Jyotsnendra Singh, Sanjoy Kumar Duttamajumder, Raman Kapur
Sugarcane ( Saccharum species hybrid) is the major source of sugar (> 80% sugar) in the world and is cultivated in more than 115 countries. It has recently gained attention as a source of biofuel (ethanol). Due to genomic complexity, the development of new genomic resources is imperative in understanding the gene regulation and function, and to fine tune the genetic improvement of sugarcane. In this study, a cDNA library was constructed from mature leaves so as to develop ESTs resources which were further compared with nucleotide and protein databases to explore the functional identity of sugarcane genes...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042620/genetic-diversity-and-population-structure-of-litsea-glutinosa-lour-in-central-india
#6
Naseer Mohammad, Ankur Dahayat, Manorama Yadav, Fatima Shirin, S A Ansari
Litsea glutinosa (Lour.), one of the most dwindling forest species in central India, is represented by highly fragmented populations that have been drastically reduced for the last 40 years, promulgating government ban on its extraction. For the first time with the help of ISSR markers, we investigated genetic variation and population structure of L. glutinosa in central Indian states. A total of 84 genotypes from 10 populations covering the entire potential pockets of the species in central India were collected...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042619/diversity-and-spatial-genetic-structure-of-natural-moroccan-quercus-susber-l-assessed-by-issr-markers-for-conservation
#7
Amal Laakili, Bouchra Belkadi, Leila Medraoui, Mohammed Alami, Chaimaa Yatrib, Ouafae Pakhrou, Mohamed Makhloufi, Salwa El Antry, Ahmed Laamarti, Abdelkarim Filali-Maltouf
Morocco is one of the most important regions of the world in terms of Quercus suber L. number and variation. This species is in decline due to several factors, which can lead to permanent loss of this resource. It would be essential to evaluate the genetic diversity in order to conserve maximum genetic variability of this species. The genetic diversity and differentiation of 16 sites from five regions representing the entire range of Moroccan Cork Oak were assessed. Twenty-three ISSR primers used generated 985 polymorphic fragments with an average of 42...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042618/molecular-insights-into-genetic-diversity-and-population-dynamics-of-five-medicinal-eulophia-species-a-threatened-orchid-taxa-of-africa
#8
Paromik Bhattacharyya, Johannes van Staden
Genetic diversity existing amongst five Eulophia orchid species were assessed using start codon targeted polymorphism (SCoT) and inter-retrotransposon amplified polymorphism (IRAP) markers. A total of 12 SCoT and 5 IRAP markers revealed an average of 63% genetic variability [SCoT = 63.87; IRAP = 64.95%] amongst the five Eulophia species investigated. The genetic similarities were assessed using both UPGMA and Bayesian approaches which indicated identical clustering patterns at a genetic similarity level of 50%...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042617/phytochemical-and-genetic-characterization-of-five-quinoa-chenopodium-quinoa-willd-genotypes-introduced-to-egypt
#9
Khalil M Saad-Allah, Mohamed S Youssef
Due to its substantial nutritional value, quinoa ( Chenopodium quinoa Willd.) is currently attracting worldwide attention. Quinoa is characterized by a high adaptability to various environmental conditions. This is the first report on the phytochemical and genetic evaluation of quinoa germplasms introduced to Egypt, and the results could be used to implement propagation techniques in the future. For phytochemical characterization, 41 traits, including primary and secondary metabolites, antioxidant molecules, sugars, organic acids and fatty acids, were evaluated...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042616/isolation-and-functional-characterization-of-the-spcbf1-gene-from-solanum-pinnatisectum
#10
Wenjiao Zhu, Ke Shi, Ruimin Tang, Xiaoying Mu, Jinghui Cai, Min Chen, Xiong You, Qing Yang
Low temperature causes a negative impact on plant growth and development, but plants evolve a series of mechanisms to respond to chilling stress, and one of them is CBF [C-repeat (CRT)/dehydration-responsive element (DRE) binding factor] gene family which has been well studied in different crops. In this paper, a new CBF1 gene, named as SpCBF1 , was isolated from frost-tolerant Solanum pinnatisectum by PCR and analyzed for its function in cold-tolerance by over-expression technique. The ORF of SpCBF1 was 666 bp long and encoded a protein of 221 amino acids with a predicted molecular mass 24...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042615/reproductive-sink-enhanced-drought-induced-senescence-in-wheat-fertile-line-is-associated-with-loss-of-antioxidant-competence-compared-to-its-cms-line
#11
Vimal Kumar Semwal, Renu Khanna-Chopra
Reproductive sinks regulate monocarpic senescence in wheat as desinking delayed flag leaf senescence under irrigated condition. In this study, wheat cv. HW 2041 and its isonuclear male sterile line (CMS) were subjected to post-anthesis water deficit stress to understand the association between sink strength, senescence and drought response in relation to oxidative stress and antioxidant defense at cellular and sub-cellular level. CMS plants maintained better water relations and exhibited delayed onset and progression of flag leaf senescence in terms of green leaf area, chlorophyll and protein content than fertile plants under water deficit stress (WDS)...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042614/exogenous-salicylic-acid-regulates-reactive-oxygen-species-metabolism-and-ascorbate-glutathione-cycle-in-nitraria-tangutorum-bobr-under-salinity-stress
#12
Yongqing Yan, Chenhui Pan, Yuling Du, Danyang Li, Wei Liu
The effect of 0.5-1.5 mM salicylic acid (SA) on modulating reactive oxygen species metabolism and ascorbate-glutathione cycle in NaCl-stressed Nitraria tangutorum seedlings was investigated. The individual plant fresh weight (PFW) and plant dry weight (PDW) significantly increased under 100 mM NaCl while remained unchanged or decreased under 200-400 mM NaCl compared to the control. Superoxide anion (O2 ·- ), hydrogen peroxide (H2 O2 ), thiobarbituric acid reactive substances (TBARS), reduced ascorbate (AsA), dehydroascorbate (DHA), reduced glutathione (GSH) and oxidized glutathione (GSSG) increased whereas the ratios of AsA/DHA and GSH/GSSG decreased under varied NaCl treatments...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042613/characterization-of-pp2a-a3-mrna-expression-and-growth-patterns-in-arabidopsis-thaliana-under-drought-stress-and-abscisic-acid
#13
Roya Razavizadeh, Behrokh Shojaie, Setsuko Komatsu
Phosphoprotein phosphatase 2A (PP2A) plays a crucial role in cellular processes via reversible dephosphorylation of proteins. The activity of this enzyme depends on its subunits. There is little information about mRNA expression of each subunit and the relationship between these gene expressions and the growth patterns under stress conditions and hormones. Here, mRNA expression of subunit A3 of PP2A and its relationship with growth patterns under different levels of drought stress and abscisic acid (ABA) concentration were analyzed in Arabidopsis thaliana ...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042612/identification-of-genes-associated-with-stress-tolerance-in-moth-bean-vigna-aconitifolia-jacq-marechal-a-stress-hardy-crop
#14
Bhavana Tiwari, Shahina Kalim, Neetu Tyagi, Ratna Kumari, Pooja Bangar, Paramananda Barman, Sanjay Kumar, Ambika Gaikwad, K V Bhat
Moth bean is the most drought and heat tolerant cultigens among Asian Vigna . We performed comparative transcriptome analysis of moth bean cultivar "Marumoth" under control and stress condition. De novo transcriptome assembly was carried out by using Velvet followed by Oases softwares. Differential expression analyses, SSR identification and validation and mapping of pathways and transcription factors were conducted. A total of 179,979 and 201,888 reads were generated on Roche 454 platform and 48,617,205 and 45,449,053 reads were generated on ABI Solid platform for the control and stressed samples...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042611/characterization-of-the-cork-formation-and-production-transcriptome-in-quercus-cerris-%C3%A3-suber-hybrids
#15
Brígida Meireles, Ana Usié, Pedro Barbosa, Ana Margarida Fortes, André Folgado, Inês Chaves, Isabel Carrasquinho, Rita Lourenço Costa, Sónia Gonçalves, Rita Teresa Teixeira, António Marcos Ramos, Filomena Nóbrega
Cork oak is the main cork-producing species worldwide, and plays a significant economic, ecological and social role in the Mediterranean countries, in particular in Portugal and Spain. The ability to produce cork is limited to a few species, hence it must involve specific regulation mechanisms that are unique to these species. However, to date, these mechanisms remain largely understudied, especially with approaches involving the use of high-throughput sequencing technology. In this study, the transcriptome of cork-producing and non-cork-producing Quercus cerris  ×  suber hybrids was analyzed in order to elucidate the differences between the two groups of trees displaying contrasting phenotypes for cork production...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30042610/strengthening-desert-plant-biotechnology-research-in-the-united-arab-emirates-a-viewpoint
#16
REVIEW
Sanjay Gairola, Khawla I Al Shaer, Eman K Al Harthi, Kareem A Mosa
The biotechnology of desert plants is a vast subject. The main applications in this broad field of study comprises of plant tissue culture, genetic engineering, molecular markers and others. Biotechnology applications have the potential to address biodiversity conservation as well as agricultural, medicinal, and environmental issues. There is a need to increase our knowledge of the genetic diversity through the use of molecular genetics and biotechnological approaches in desert plants in the Arabian Gulf region including those in the United Arab Emirates (UAE)...
July 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692558/identification-of-candidate-resistance-genes-of-cotton-against-aspergillus-flavus-infection-using-a-comparative-transcriptomics-approach
#17
Muthamilarasan Mehanathan, Renesh Bedre, Venkata Mangu, Kanniah Rajasekaran, Deepak Bhatnagar, Niranjan Baisakh
A comparative transcriptome analysis was performed using the genes significantly differentially expressed in cotton, corn and peanut in response to aflatoxin producing fungus Aspergillus flavus with an objective of identifying candidate resistance genes in cotton. Two-way analyses identified 732 unique genes to be differentially regulated by the fungus with only 26 genes common across all three crops that were considered candidate A. flavus resistance genes with an assumption that these genes have specific roles in conferring the resistance trait...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692557/agroinfiltration-a-rapid-and-reliable-method-to-select-suitable-rose-cultivars-for-blue-flower-production
#18
Masoume Zeinipour, Pejman Azadi, Ahmad Majd, Maryam Jafarkhani Kermani, Saeed Irian, Seyed Mohammad Hosseini, Masahiro Mii
Rose cultivars with blue flower color are among the most attractive breeding targets in floriculture. However, they are difficult to produce due to the low efficiency of transformation systems, interactive effects of hosts and vectors, and lengthy processes. In this study, agroinfiltration-mediated transient expression was investigated as a tool to assess the function of flower color genes and to determine appropriate host cultivars for stable transformation in Rosa hybrida . To induce delphinidin accumulation and consequently to produce blue hue, the petals of 30 rose cultivars were infiltrated with three different expression vectors namely pBIH-35S-CcF3'5'H, pBIH-35S-Del2 and pBIH-35S-Del8, harbouring different sets of flower color genes...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692556/assessment-of-genetic-diversity-and-population-structure-in-gladiolus-gladiolus-hybridus-hort-by-issr-markers
#19
Veena Chaudhary, Mukesh Kumar, Shailendra Sharma, Navneet Kumar, Vipin Kumar, Hemant Kumar Yadav, Shiveta Sharma, Ujjwal Sirohi
ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus ( Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692555/genetic-diversity-and-structure-of-capparis-spinosa-l-in-iran-as-revealed-by-issr-markers
#20
Maryam Ahmadi, Hojjatollah Saeidi
Capparis spinosa L. (caper bush) is an economically and ecologically important perennial shrub that grows across different regions of Iran. In this study, the genetic diversity and population structure of Iranian genepool of C. spinosa is evaluated using Inter Simple Sequence Repeat (ISSR) markers. Using 10 ISSR primers, 387 DNA fragments (bands) were amplified from the genomic DNA of 92 individuals belonging to twenty-one populations of C . spinosa , of which 378 (97.7%) were polymorphic. High level of genetic diversity (percentage of polymorphic loci = 98...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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