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Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology

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https://www.readbyqxmd.com/read/30150872/determination-of-critical-low-light-limit-and-adaptive-physiological-and-biochemical-traits-regulating-growth-and-yield-of-mustard-brassica-juncea-coss
#1
Badre Alam, Rashmi Singh, Mayank Chaturvedi, Ram Newaj, O P Chaturvedi
Growth and physio-biochemical traits under different incident solar light intensities (100, 67, 50 and 25%) were studied in mustard in a semi-arid agroclimate region of Central India. Our comprehensive studies revealed that incident solar light intensities below about 67% were highly detrimental in mustard for its growth and grain yield. Major factors that contributed to the differential responses under varying light intensities were identified which holds importance for better understanding of low light adaptability in an important oilseed crop like mustard...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150871/genotypic-variation-of-photosynthetic-gas-exchange-and-stomatal-traits-in-some-traditional-rice-oryza-sativa-l-landraces-from-koraput-india-for-crop-improvement
#2
Debabrata Panda, Amar Mahakhud, Biswajeet Mohanty, Swati S Mishra, Jijnasa Barik
Variations in photosynthetic gas exchange, stomatal traits and photosystem (PS) II activity were investigated in three popular rice ( Oryza sativa L.) landraces namely Kalajeera, Machakanta and Haladichudi from Koraput, India and compared with high yielding modern varieties (IR 64 and IR 42) to judge the possibility of using them in crop improvement programmes. The leaf CO2 photosynthetic rate (PN ), stomatal conductance (gs), water use efficiency and carboxylation efficiency were significantly higher in traditional landraces as compared to the high yielding variety...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150870/calcium-alginate-encapsulated-synthetic-seed-production-in-plumbago-rosea-l-for-germplasm-exchange-and-distribution
#3
Anand Vishnu Prakash, Deepa S Nair, Swapna Alex, K B Soni, M M Viji, B R Reghunath
Plumbago rosea L. (Plumbaginaceae), is a medicinal shrub commercially exploited for its naphthoquinone principle, plumbagin, extracted from the roots especially for treating skin disorders. As the plant is exploited from the wild without being replenished, conservation of the species becomes inevitable. Synthetic seeds would provide for effective conservation, germplasm exchange and distribution of this species. A reliable protocol for synthetic seed production in Plumbago rosea has been developed encapsulating the axillary buds...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150869/development-of-an-efficient-in-vitro-mutagenesis-protocol-for-genetic-improvement-of-saffron-crocus-sativus-l
#4
Mahpara Kashtwari, Aijaz A Wani, Manoj K Dhar, Sabbi Jan, Azra N Kamili
Saffron ( Crocus sativus L) is a triploid (2n = 3x = 24), sterile geophyte which can only be propagated by means of underground vegetative corms. Since corm multiplication does not induce genome variations, therefore, the entire saffron population is expected to have a similar genetic makeup. Keeping in view the economic importance of the plant and the factors responsible for its low yield, the present investigation has been undertaken to establish an in vitro ethyl methanesulfonate (EMS) mutagenesis protocol followed by characterization of the induced variability in the advanced generations...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150868/genetic-diversity-of-superior-persian-walnut-genotypes-in-azadshahr-iran
#5
Fatemeh Shamlu, Mehdi Rezaei, Shaneka Lawson, Aziz Ebrahimi, Abbas Biabani, Alireza Khan-Ahmadi
Persian walnut ( Juglans regia L.) is known to have originated in central and eastern Asia. Remnants of these wild populations can still be found in the Hyrcanian forest in north-eastern Iran. In this study, 102 individual walnut trees from four geographic populations in the Azadshahr province (Vamenan, Kashidar, Rudbar and Saidabad) were sampled. We characterized individual trees using 28 standard morphological traits. The range of traits varied widely for some economically important characteristics including nut weight (6...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150867/ginmicrosatdb-a-genome-wide-microsatellite-markers-database-for-sesame-sesamum-indicum-l
#6
Supriya Purru, Sarika Sahu, Saurabh Rai, A R Rao, K V Bhat
Molecular breeding in sesame is still at infancy due to limited number of microsatellite markers available and the low level of polymorphism exhibited by them. Therefore, whole genome sequencing was used for development of microsatellite markers so as to ensure availability of substantial number of polymorphic markers for use in marker assisted breeding programs. Whole genome sequencing of sesame variety 'Swetha' was done using Illumina paired-end sequencing and Roche 454 shotgun sequencing technologies (GCA_000975565...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150866/easy-strategy-used-to-detect-the-genetic-variability-in-chickpea-cicer-arietinum-l
#7
E Valadez-Moctezuma, A J Cabrera-Hidalgo
A priority in the management and use of elite plant materials for breeding has been based on molecular markers or DNA sequencing of entire genomes, in order to perform genetic differentiation which is still quite costly. Chickpea ( Cicer arietinum ) is one of the species with genomic monotony and very low polymorphism, and its detection even with DNA markers has not been easy. In germplasm banks, the genetic distinction is a priority in order to use properly selected lines. In this study, 57 chickpea accessions from a germplasm bank were analyzed by using nrRAMP (non-radioactive Random Amplified Microsatellite Polymorphism) markers, and their genetic variability was determined...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150865/qtl-mapping-for-some-grain-traits-in-bread-wheat-triticum-aestivum-l
#8
Supriya Kumari, Vandana Jaiswal, Vinod Kumar Mishra, Rajneesh Paliwal, Harindra Singh Balyan, Pushpendra Kumar Gupta
Grain traits are important agronomic attributes with the market value as well as milling yield of bread wheat. In the present study, quantitative trait loci (QTL) regulating grain traits in wheat were identified. Data for grain area size (GAS), grain width (GWid), factor form density (FFD), grain length-width ratio (GLWR), thousand grain weight (TGW), grain perimeter length (GPL) and grain length (GL) were recorded on a recombinant inbred line derived from the cross of NW1014 × HUW468 at Meerut and Varanasi locations...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150864/phytochemical-and-antioxidative-potential-of-orange-red-yellow-rainbow-and-black-coloured-tropical-carrots-daucus-carota-subsp-sativus-schubl-martens
#9
B K Singh, T K Koley, Arti Maurya, P M Singh, B Singh
This study was executed to determine phytochemical content i.e. total carotenoids, phenolics and flavonoids, and antioxidant ability expressed in the form of FRAP, CUPRAC and ABTS activity among different coloured tropical carrots (orange, red, yellow, rainbow and black carrot) developed at ICAR-IIVR, Varanasi, Uttar Pradesh, India. Overall, within different colour group, the extent of variation for various phytochemical content and antioxidant potentiality is narrow i.e. ranged from 1.04- to 3.21-fold; but at the same time, the genotypic variability across genotypes is too wide which varied 20...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150863/enhancement-of-total-phenolic-and-flavonoids-extraction-from-rosmarinus-officinalis-l-using-electromagnetic-induction-heating-emih-process
#10
Sarah Megateli, Mohamed Krea
Electromagnetic induction heating (EMIH) assisted extraction of phenolic compounds from Rosmarinus officinalis L, and the antioxidant and antimicrobial activities of the plant extract were examined in this study. The extraction yield acquired with this process was found to be 25.1 ± 2%, with maximum amounts of phenolic compounds: 127.87 ± 2.1 mg Gallic acid equivalents per g dry weight and total flavonoids contents 14.48 ± 1.5 mg quercetin equivalents per g dry weight, under optimum extraction conditions (extraction time 2 h, ratio of raw material to liquid 1:2 and 0% of NaCl)...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150862/impact-of-maturity-on-phenolic-composition-and-antioxidant-activity-of-medicinally-important-leaves-of-ficus-carica-l
#11
Muhammad Nadeem, Alam Zeb
Ficus carica L. is one of the oldest trees belonging to the mulberry family. It has both nutritional and medicinal benefits, as it is a source of potential bioactive compounds. Fig leaves were collected at four maturation stages, to evaluate the variation in polyphenolic compounds, antioxidant activity, and different pigments level. Polyphenols were identified using HPLC-DAD. Different pigments level, total phenolic contents, and radical scavenging activity were quantified. The HPLC analysis revealed the presence of fourteen polyphenolic compounds in the fig leaves...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150861/biochemical-and-molecular-studies-on-the-resistance-mechanisms-in-tea-camellia-sinensis-l-o-kuntze-against-blister-blight-disease
#12
Sam Nirmala Nisha, Gajjeraman Prabu, Abul Kalam Azad Mandal
Tea ( Camellia sinensis ) plantations are exposed to biotic and abiotic stresses. Among the biotic factors, blister blight (BB), caused by Exobasidium vexans , affects the quality and quantity of the product and demands high fungicide application. A long term solution for disease resistance would require the knowledge of the basic molecular and biochemical changes occurring in plant as an attempt to resist the pathogen and limit the spread of the disease which can further help in developing resistant cultivars using biotechnological tools...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150860/effects-of-chilling-stress-on-the-accumulation-of-soluble-sugars-and-their-key-enzymes-in-jatropha-curcas-seedlings
#13
Haibo Wang, Ming Gong, Hu Xin, Lizhou Tang, Dongqin Dai, Yong Gao, Chao Liu
As osmolytes and signaling molecules, soluble sugars participate in the response and adaptation of plants to environmental stresses. In the present study, we measured the effect of chilling (12 °C) stress on the contents of eight soluble sugars in the leaves, cotyledons, stems, and roots of Jatropha curcas seedlings, as well as on the activities of eight rate-limiting enzymes that are critical to the metabolism of those soluble sugars. Chilling stress promoted both starch hydrolysis and soluble sugar accumulation...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150859/terminal-drought-and-seed-priming-improves-drought-tolerance-in-wheat
#14
Tahira Tabassum, Muhammad Farooq, Riaz Ahmad, Ali Zohaib, Abdul Wahid, Muhammad Shahid
Plants retain the preceding abiotic stress memory that may aid in attainment of tolerance to subsequent stresses. This study was conducted to evaluate the influence of terminal drought memory (drought priming) and seed priming in improving drought tolerance in wheat ( Triticum aestivum L.). During first growing season, wheat was planted in field under optimal (well-watered) and drought stress imposed at reproductive stage (BBCH growth stage 49) until maturity (BBCH growth stage 83). Seeds collected from both sources were subjected to hydropriming or osmopriming (with 1...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150858/change-in-antioxidant-enzymes-activity-and-some-morpho-physiological-characteristics-of-strawberry-under-long-term-salt-stress
#15
Nasser Ghaderi, Mohamad Reza Hatami, Aliakbar Mozafari, Adel Siosehmardeh
The effects of long term salinity on some morpho-physiological characteristics were studied in strawberry Kurdistan and Queen elisa cultivars. Vegetative and biochemical traits were measured in strawberry cultivars subjected to three levels of salinity including 0, 40 and 80 mM at 20, 40 and 60th days after NaCl addition. Results showed that in both cultivars the dry weight of plant organs decreased in response to NaCl, except of crown weight in cv. Kurdistan. Root to shoot ratio increased due to a greater reduction in above ground biomass under salinity...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150857/characterization-of-brassinazole-resistant-bzr-gene-family-and-stress-induced-expression-in-eucalyptus-grandis
#16
Chunjie Fan, Guangsheng Guo, Huifang Yan, Zhenfei Qiu, Qianyu Liu, Bingshan Zeng
Brassinosteroids (BRs) are a group of plant hormones which play a pivotal role in modulating cell elongation, stress responses, vascular differentiation and senescence. In response to BRs, BRASSINAZOLE-RESISTANT (BZR) transcription factors (TFs) accumulate in the nucleus, where they modulate thousands of target genes and coordinate many biological processes, especially in regulating defense against biotic and abiotic stresses. In this study, 6 BZR TFs of Eucalyptus grandis ( EgrBZR ) from a genome-wide survey were characterized by sequence analysis and expression profiling against several abiotic stresses...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150856/the-vacuolar-proton-pyrophosphatase-gene-sbvppase-from-the-sorghum-bicolor-confers-salt-tolerance-in-transgenic-brahmi-bacopa-monnieri-l-pennell
#17
M L Ahire, S Anil Kumar, D L Punita, P S Mundada, P B Kavi Kishor, T D Nikam
Plants overcome the effect of Na+ toxicity either by excluding Na+ at the plasma membrane or by sequestering them into the vacuoles. Influx of Na+ ions into the plant vacuoles is usually driven by H+ generated by vacuolar-type H+ -ATPase as well as vacuolar proton pyrophosphatse (VPPase). In the present study, we have developed Bacopa monnieri transgenics via Agrobacterium tumefaciens containing the recombinant vector pCAMBIA2300- SbVPPase gene. Transformants were produced using nodal explants. Transformants were confirmed by PCR and DNA blot analysis...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150855/identification-of-suitable-internal-control-genes-for-transcriptional-studies-in-eleusine-coracana-under-different-abiotic-stress-conditions
#18
Pradeep K Jatav, Ankita Sharma, Dinesh K Dahiya, Arif Khan, Atika Agarwal, S L Kothari, Sumita Kachhwaha
Finger millet [ Eleusine coracana (L.) Gaertn] is an excellent food and forage crop of arid and semiarid areas in Africa and Asia. It is well adapted to drought, heat, high salinity, poor soil fertility and low pH with an efficient C4 carbon fixation mechanism for high yield potential. To normalize the target gene expression data, the identification of suitable reference genes is essential. Ten candidate reference genes were selected and their expression stability was analyzed in various samples treated with different abiotic stress conditions...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150854/selection-of-reference-genes-for-reverse-transcription-qpcr-analysis-in-the-biomonitor-macrophyte-bidens-laevis-l
#19
Germán Lukaszewicz, María Valeria Amé, Mirta Luján Menone
The RT-qPCR has been the method used to analyze gene expression in plants but its benefits have not been completely exploited in the field of plants ecotoxicology when used as molecular biomarkers. The correct use of RT-qPCR demands to establish a certain number of reference genes (RG) which are expected to be invariable in their expression although it does not always happen. The main goals of this work were to: (1) analyze the stability of six potential RG, (2) establish the optimum number of RG, (3) select the most suitable RG to be applied in Bidens laevis under different test conditions and tissues and (4) confirm its convenience by normalizing the expression of one gene of interest under three different challenges...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30150853/validation-of-reference-genes-for-rt-qpcr-studies-in-stevia-rebaudiana-in-response-to-elicitor-agents
#20
Simone Ribeiro Lucho, Marcelo Nogueira do Amaral, Letícia Carvalho Benitez, Cristini Milech, Alitcia Moraes Kleinowski, Valmor João Bianchi, Eugenia Jacira Bolacel Braga
Stevia rebaudiana is an important source of natural steviol glycosides and is of increasing interest in various fields of study. Therefore, understanding the molecular processes regulating its metabolism is of great importance. In this study, the stability of seven reference genes ( 18S ribosomal RNA, Actin, Aquaporin, Calmodulin, Eukaryote elongation factor 1 - α, Malate dehydrogenase, and Ubiquitin ) under the effect of three stress-related elicitors (methyl jasmonate, salicylic acid, and spermidine) was evaluated in stevia plants...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
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