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IET Nanobiotechnology

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June 2018: IET Nanobiotechnology
Shivangi Goyal, Nidhi Gupta, Ajeet Kumar, Sreemoyee Chatterjee, Surendra Nimesh
In this study, the authors report a simple and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using Trigonella foenum-graecum (TFG) seed extract. They explored several parameters dictating the biosynthesis of TFG-AgNPs such as reaction time, temperature, concentration of AgNO3 , and TFG extract amount. Physicochemical characterisation of TFG-AgNPs was done on dynamic light scattering (DLS), field emission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy...
June 2018: IET Nanobiotechnology
Neda Alamdar, Behnam Rasekh, Fatemeh Yazdian
The aim of this study was to evaluate the effects of iron (Fe)/SDS and gold (Au) nanoparticles on growth and biosurfactant production of Pseudomonas aeruginosa PBCC5 . The concentrations of the nanoparticles used were 1, 500 and 1000 mg/l. In this research, the surface tension of biosurfactant, dry weight of biosurfactant and biomass, emulsification indexes (E24 ) were measured and transmission electron microscopy analysis was used to monitor the nanoparticles. The test results showed that the effect of nanoparticles on the bacterial growth and biosurfactant production varied corresponding to the type and concentration of nanoparticles...
June 2018: IET Nanobiotechnology
Mubashir Hussain, Naveed Iqbal Raja, Zia-Ur-Rehman Mashwani, Farah Naz, Muhammad Iqbal, Sumaira Aslam
The synthesis of nanoparticles by utilising plant extract has revolutionised the field of nanotechnology. In the present study, AgNPs were synthesised by utilising the leaves of Moringa oleifera as reducing and stabilising agent. UV-visible spectroscopy showed characteristic surface plasmon band in the range of 413-420 nm. Scanning electron microscopy (SEM) elucidated rectangular segments fused together. X-ray diffraction (XRD) analysis confirmed the crystalline nature of AgNPs and presence of metallic silver ions was confirmed by energy dispersive X-ray (EDX)...
June 2018: IET Nanobiotechnology
Ravi Mani Tripathi, Braj Raj Shrivastav, Archana Shrivastav
This study reveals the antibacterial and catalytic activity of biogenic gold nanoparicles (AuNPs) synthesised by biomass of Trichoderma harzianum . The antibacterial activity of AuNPs was analysed by the means of growth curve, well diffusion and colony forming unit (CFU) count methods. The minimum inhibitory concentration of AuNPs was 20 µg/ml. AuNPs at 60 µg/ml show effective antibacterial activity as optical absorption was insignificant. The well diffusion and CFU methods were also applied to analyse the effect of various concentration of AuNPs...
June 2018: IET Nanobiotechnology
Seetharaman Prabukumar, Chandrasekaran Rajkuberan, Gnanasekar Sathishkumar, Mani Illaiyaraja, Sivaperumal Sivaramakrishnan
In this study, the leaf extract of an important medicinal plant Crescentia cujete L. (CC) was employed as a green reducing agent to synthesise highly-stable C. cujete silver nanoparticles (CCAgNPs). The reduction of Ag+ to Ag0 nanoparticles was initially observed by a colour change which generates an intense surface plasmon resonance peak at 417 nm using a UV-Vis spectrophotometer. Various optimisation factors such as temperature, pH, time and the stoichiometric proportion of the reaction mixture were performed, which influence the size, dispersity and synthesis rate of CCAgNPs...
June 2018: IET Nanobiotechnology
Zhaoyang Xu, Xiangdong Jiang, Sicong Tan, Weibing Wu, Jiangtao Shi, Huan Zhou, Peng Chen
In recent years, carbon aerogels have attracted much attention in basic research and as potential applications in many fields. Herein, the authors report a novel approach using bamboo powder as raw material to fabricate cellulose nanofibers (CNFs)/multi-walled carbon nanotubes (MWCNTs) carbon aerogels by a simple dipping and carbonisation process. The developed material exhibits many exciting properties including low density (0.056 g cm-3 ), high porosity (95%), efficient capability for separation of oily droplets from water, and high adsorption capacity for a variety of oils and organic solvents by up to 110 times its own weight...
June 2018: IET Nanobiotechnology
Abhishek R Indurkar, Viraj D Sangoi, Prashant B Patil, Mansingraj S Nimbalkar
Here, the authors report a rapid, simple, and eco-friendly process for synthesis of Bi2 O3 nano-needles. Dioscorea alata tuber extract was used as both reducing and capping agent for the first time. These nanoparticles were characterised by X-ray diffraction, field emission scanning electron microscope, and Fourier transform infrared (FTIR) spectrometry, the nano-structured Bi2 O3 needles have an average diameter of 158 nm with the lengths in the range of 1-3 μm. CLSI M27-A2 standard was followed for evaluation of anti-fungal activity...
June 2018: IET Nanobiotechnology
Fakhte Zandpour, Ali Reza Allafchian, Mohammad Reza Vahabi, Seyed Amir Hossein Jalali
Silver nanoparticles (SNPs) were synthesised by using the Arial part extract of Dorema ammoniacum D . and characterised by employing UV-visible spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction techniques. Transmission electron microscopy and field emission scanning electron microscopy were applied to investigate the morphological structure of the bio-synthesised SNPs. The antimicrobial activity of SNPs was studied against Gram positive ( Bacillus cereus and Staphylococcus aureus ) and Gram-negative ( Escherichia coli and Salmonella typhimurium ) bacteria by employing the disk diffusion agar process...
June 2018: IET Nanobiotechnology
Maryam Taajobian, Ali Jahanian
Digital microfluidic is an emerging technology to reduce the cost and time of experiments and improve the flexibility, automate-ability and correctness of biochemical assays. In many of applications such as drug discovery and DNA profiling, a large number of bio-operations (e.g. the chemical operations used in biology applications) must be done. In these applications, parallelising the operations will be critical in accuracy and cost of the process and digital microfluidic biochips can be considered as a reasonable platform...
June 2018: IET Nanobiotechnology
Asmathunisha Nabikhan, Suji Rathinam, Kathiresan Kandasamy
The present study investigated the synthesis of gold nanoparticles (AuNPs) using mangrove plant extract from Avicennia marina as bioreductant for eco-friendly bioremediation of 4-nitrophenol (4-NP) . The AuNPs synthesised were confirmed by UV spectrum, transmission electron microscopy (TEM), X-ray diffraction, Fourier transmission infrared spectroscopy (FTIR), dynamic light scattering (DLS), and zeta potential. The AuNPs were found to be spherical in shape with size ranging from 4 to 13 nm, as evident by TEM and DLS...
June 2018: IET Nanobiotechnology
Sravani Kantipudi, Jhansi Rani Sunkara, Muralikrishna Rallabhandi, Chandi Vishala Thonangi, Raga Deepthi Cholla, Pratap Kollu, Madhu Kiran Parvathaneni, Sri Venkata Narayana Pammi
In the present study, silver (Ag) and Ag-zinc oxide (ZnO) composite nanoparticles (NPs) were synthesised and studied their wound-healing efficacy on rat model. Ultraviolet-visible spectroscopy of AgNPs displayed an intense surface plasmon (SP) resonance absorption at 450 nm. After the addition of aqueous Zn acetate solution, SP resonance band has shown at 413.2 nm indicating a distinct blue shift of about 37 nm. X-ray diffraction analysis Ag-ZnO composite NPs displayed existence of two mixed sets of diffraction peaks, i...
June 2018: IET Nanobiotechnology
Jaleh Varshosaz, Negin Anvari
Acute lymphoblastic leukemia (ALL) is the white blood cell cancer in children. L-asparaginase (L-ASNase) is one of the first drugs used in ALL treatment. Anti-tumor activity of L-ASNase is not specific and indicates limited stability in different biological environments, in addition to its quick clearance from blood. The purpose of the present study was to achieve a new L-ASNase polymer bioconjugate to improve pharmacokinetic, increase half-life and stability of the enzyme. The conjugations were achieved by the cross-linking agent of 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) which activates the carboxylic acid groups of polymeric nanoparticles to create amide bond...
June 2018: IET Nanobiotechnology
Karthik Subramani, Balu Kolathupalayam Shanmugam, Suriyaprabha Rangaraj, Manivasakan Palanisamy, Prabu Periasamy, Rajendran Venkatachalam
Nanomaterials play a vital role in textile industries due to their unique properties and applications. There is an increase in the use of nanoscale phyto products in textiles to control the bacterial infection in fabrics. Here, natural herbal nanoparticles of different sizes were prepared from shade-dried Aloe vera plant leaves using ball milling technique without any additives. The amorphous herbal A. vera nanoparticles possess an average particle size of 40 ± 2 nm and UV-absorption maximum at 269 nm...
June 2018: IET Nanobiotechnology
Aghil Esmaeili-Bandboni, Seyed Mohammad Amini, Reza Faridi-Majidi, Jamshid Bagheri, Javad Mohammadnejad, Esmaeil Sadroddiny
MiR-155 plays a critical role in the formation of cancers and other diseases. In this study, the authors aimed to design and fabricate a biosensor based on cross-linking gold nanoparticles (AuNPs) aggregation for the detection and quantification of miR-155. Also, they intended to compare this method with SYBR Green real-time polymerase chain reaction (PCR). Primers for real-time PCR, and two thiolated capture probes for biosensor, complementary with miR-155, were designed. Citrate capped AuNPs (18.7 ± 3...
June 2018: IET Nanobiotechnology
Qiang Liu, Jiao Wang, Linnan Yang, Xiaofei Xia, Mei Wang, Shengguang Chen, Rongrong Zhu, Qingxiu Wang, Xianzheng Wu, Shilong Wang
In this study, a promising drug nano-carrier system consisting of mono-dispersed and pH sensitive carboxylated chitosan-hollow mesoporous silica nanoparticles (Ccs-HMSNs) suitable for the treatment of malignant cells was synthesised and investigated. At neutral pH, the Ccs molecules are orderly aggregated state, which could effectively hinder the release of loaded drug molecules. However, in slightly acidic environment, Ccs chains are heavily and flexibly entangled in gel state, which would enhance the subsequent controlled release of the loaded drug...
June 2018: IET Nanobiotechnology
Xiaojie Song, Xianyang Shi, Mi Yang
Biosynthesised nanocomposites have attracted growing interests attributed to their 'green' synthesis nature in recent years. Shewanella oneidensis MR-1, a dissimilatory metal-reducing bacterium, was used to reduce palladium (II) nitrate to palladium (0) nanoparticles (Pd NPs) under anaerobic conditions, resulting in the in situ formation of Pd NPs immobilised on TiO2 nanotubes (TNTs) (Pd/TNTs nanocomposites). The Pd/TNTs nanocomposites were characterised by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, energy dispersive X-ray, and electron spin resonance, respectively...
June 2018: IET Nanobiotechnology
Shaghaegh Amirijavid, Maliheh Entezari
Cancer treatment with several kinds of drugs, especially targets the apoptotic pathways nowadays. TNF-related apoptosis-inducing ligand (TRAIL) as one of the important members of death receptors, significantly trigger induction of apoptosis in cancer cells. Three conserved domains of Death receptor (DR5) protein extracellular domain, which are fortified cysteine, were chosen and chemically synthesised. Hens were immunised with nano-liposomal peptides, and as a result the purified Immunoglobulin (IgYs) remarkably killed the cancerous MCF7 cells...
June 2018: IET Nanobiotechnology
Thivyah Munisparan, Evyan Chia Yan Yang, Ragul Paramasivam, Nuraina Anisa Dahlan, Janarthanan Pushpamalar
Ultrafine titanium dioxide (TiO2 ) nanowires were synthesised using a hydrothermal method with different volumes of ethylene glycol (EG) and annealing temperatures. It shows that sodium titanate nanowires synthesised using 5 and 10 ml EG, which annealed at 400°C produced TiO2 nanowires that correspond to a photochemically active phase, which is anatase. The influences of annealing temperatures (400-600°C) on the morphological arrangement of TiO2 nanowires were evident in the field emission scanning electron microscopy...
June 2018: IET Nanobiotechnology
Komkrich Sawasdee, Jeerus Sucharitakul, Taweesak Dhammaraj, Nuttawee Niamsiri, Pimchai Chaiyen, Kanlaya Prapainop
p -Hydroxyphenylacetate 3-hydroxylase component 1 ( C 1 ) is a useful enzyme for generating reduced flavin and NAD+ intermediates. In this study, poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were used to encapsulate the C 1 (PLGA- C 1 NPs). Enzymatic activity, stability, and reusability of PLGA- C 1 NPs prepared using three different methods [oil in water (o/w), water in oil in water (w/o/w), and solid in oil in water (s/o/w)] were compared. The s/o/w provided the optimal conditions for encapsulation of C 1 (PLGA- C 1,s NPs), giving the highest enzyme activity, stability, and reusability...
June 2018: IET Nanobiotechnology
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