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International Journal of Laboratory Hematology

Vaughan K Williams
INTRODUCTION: Point-of-care (POC) international normalized ratio (INR) values above an institutional cutoff are confirmed in the laboratory using a gold standard venous specimen. This can be problematic in a pediatric setting. METHOD: In this study, 449 consecutive POC INR results were compared to an INR performed in the laboratory on a capillary citrate specimen collected from the same finger-stick. The results were statistically analyzed. RESULTS: The mean INR values from the CoaguChek XS and laboratory were 2...
September 14, 2018: International Journal of Laboratory Hematology
Shira Ronen, Steven H Kroft, Horatiu Olteanu, Paul R Hosking, Alexandra M Harrington
INTRODUCTION: Carcinocythemia is a rare phenomenon defined as morphologically identifiable, circulating tumor cells in the peripheral blood. No modern case series of carcinocythemia exists in the literature. METHODS: Blood smears from carcinocythemia patients were reviewed and associated clinicopathologic findings described and compared to the literature. When available, bone marrows were examined. RESULTS: We identified 7 carcinocythemia cases over 3 years at our institution in 5 females and 2 males with a median age of 57 and compare them to 26 case reports in the literature (19 females, 10 males; median age 57)...
September 14, 2018: International Journal of Laboratory Hematology
Nasir Al-Allawi, Shatha M A Qadir, Helene Puehringer, David H K Chui, John J Farrell, Christian Oberkanins
INTRODUCTION: Fetal hemoglobin (HbF) is the major modifier for sickle cell disease (SCD) severity. HbF is modulated mainly by three major quantitative trait loci (QTL) on chromosomes 2, 6, and 11. METHODS: Five SNPs in the three QTLs (HBG2, rs7482144; BCL11A, rs1427407 and rs10189857; and HBS1L-MYB intergenic region, rs28384513 and rs9399137) were investigated by multiplex PCR and reverse hybridization, and their roles in HbF and clinical phenotype variability in Iraqi Kurds with SCD were assessed...
September 14, 2018: International Journal of Laboratory Hematology
Wiralpat Padungmaneesub, Sanit Reungrongrat, Suphara Manowong, Kanda Fanhchaksai, Noppamas Panyasit, Rungrote Natesirinilkul
INTRODUCTION: Disseminated intravascular coagulation (DIC) is a systemic activation of hemostatic system caused by several causes. Biomarkers including antithrombin (AT), protein C (PC), and thrombomodulin (TM) were reported as the additional markers for DIC in adults. This study aimed to determine the association between biomarkers among patients with overt DIC (ODIC) and nonovert DIC (NDIC) in children in PICU. METHODS: We enrolled 103 subjects, aged 1 month-18 years, who were admitted to PICU at Chiang Mai University (CMU) Hospital >24 hours with underlying conditions predisposing to DIC were enrolled...
September 12, 2018: International Journal of Laboratory Hematology
Yu Fu, Rui Zhang, Qisheng Wu, Jiawei Zhang, Lihua Bao, Jinming Li
INTRODUCTION: External quality assessment (EQA) is an essential tool for quality assurance of analytical testing processes of p210 BCR-ABL1 transcripts by RT-qPCR. As an EQA provider, the National Center for Clinical Laboratories organized an EQA scheme of p210 BCR-ABL1 testing in China for the first time to identify existing problems and ensure the reliability of p210 BCR-ABL1 testing. METHODS: Using armored RNA technology, we first constructed pACYC-MS2-p210 and CG recombinant plasmids and expressed p210 and CG armored RNAs, with packaging segments of p210 BCR-ABL1 fusion gene (FG) and four common control gene (CG) transcripts...
September 11, 2018: International Journal of Laboratory Hematology
Cyril Jacquot, Geoffrey D Wool, Scott C Kogan
INTRODUCTION: The dilute Russell viper venom time (dRVVT) detects lupus anticoagulant (LA). International Society for Thrombosis and Haemostasis (ISTH) guidelines specify positivity criteria, which differ from the assay manufacturer's criteria. METHODS: Two years of dRVVT testing at our institution were reviewed. For patients with prolonged dRVVT screening times, we evaluated dRVVT results by ISTH and manufacturer's criteria and correlated with the results of other antiphospholipid syndrome (APS) testing (LA-sensitive activated partial thromboplastin time and antiphospholipid antibodies) and with history of thromboembolism and other APS manifestations...
September 8, 2018: International Journal of Laboratory Hematology
Ian Jennings, Dianne Kitchen, Steve Kitchen, Timothy Woods, Isobel Walker
External Quality Assessment (EQA) is an important part of laboratory quality assurance. Spiking of normal plasma is sometimes employed to mimic clinical samples. It is important that spiked material gives similar results to clinical samples (ie, is commutable) to ensure appropriate conclusions can be drawn from EQA exercises. We describe here data from UK National External Quality Assessment Scheme for Blood Coagulation (NEQAS BC) exercises where spiked samples were tested alongside samples from patients to explore commutability of artificial material...
September 5, 2018: International Journal of Laboratory Hematology
Victor Bobée, Sylvie Daliphard, Aurélien Schrapp, Agnès Lahary
INTRODUCTION: Development of additional parameters for complete blood count has emerged in recent hematology analyzers, leading to many publications. However, few studies have been conducted on advanced RBC parameters and hemolytic anemias. We investigated the interest of Sysmex unique parameters, MicroR and HypoHe, as well as the immature fraction of reticulocytes (IRF) in combination with complete blood and reticulocyte count, for screening hereditary spherocytosis (HS) and pyruvate kinase deficiency...
September 5, 2018: International Journal of Laboratory Hematology
Adriaan J van Gammeren, M van Rijckevorsel, Anne E Bras, Claudia Brandt-Hagens, Jeroen G Te Marvelde, Carin de Visser, Annemiek M C P Joosen, Anton A M Ermens, Vincent H J van der Velden
BACKGROUND: A flow cytometry score (FCS) based on four parameters has been proposed for analysis of myelodysplastic syndromes patients with <5% blasts. We evaluated whether bone marrow aspirate samples isolated from femoral heads could be used for verification of cutoff values of the individual parameters score (IPS), contributing to the FCS and compared the applicability of FCS parameters in two centers. STUDY DESIGN AND METHODS: Bone marrow cells were obtained from femoral heads of patients who underwent hip replacement surgery...
September 2, 2018: International Journal of Laboratory Hematology
Julie K Weisman, Daveena Meeks, Laurel Mendelsohn, Alan T Remaley, Maureen Sampson, Darlene T Allen, Jim Nichols, Arun S Shet, Swee Lay Thein
INTRODUCTION: Sickle cell disease (SCD) is a multisystemic disorder, the pathology being driven by recurrent inflammation particularly during a vaso-occlusive crisis. GlycA, a composite measure of protein glycation, is a sensitive biomarker for disorders associated with vascular inflammation. We determined the utility of GlycA as a biomarker of inflammation in SCD. METHODS: Stored plasma samples from patients with SCD recruited to two clinical studies were analyzed...
August 27, 2018: International Journal of Laboratory Hematology
Anna Johnston, Gini Bourner, Tracy Martin, Andrew McFarlane, David Good, Ruth Padmore, Anne Raby, Berna Aslan
INTRODUCTION: Effective medical laboratory quality management systems ensure confidence in analyzing and reporting accurate and reliable patient results. To guarantee quality assurance, each laboratory needs appropriate internal quality control (IQC) procedures to monitor their test systems. The Institute for Quality Management in Healthcare (IQMH) Centre for Proficiency Testing conducted a survey on quality control (QC) practices in routine hematology. METHODS: An online survey was sent to 184 Ontario laboratories performing complete blood counts (CBC) and leukocyte differentials...
August 23, 2018: International Journal of Laboratory Hematology
Mesude Falay, Mehmet Senes, Selcuk Korkmaz, Turan Turhan, Murat Okay, Berna Afacan Öztürk, Doğan Yücel, Gulsum Ozet
BACKGROUND: Although tests of global hemostasis prothrombin time (PT) and activated partial thromboplastin time (aPTT) should not be used for prediction of bleeding risk, these tests are often used by many clinicians in daily practice particularly as a preoperative screening test. Robust biological variation (BV) data are needed for safe clinical applications of these tests. In this study, a stringent protocol was followed to estimate the BV's for PT, aPTT, and fibrinogen levels. METHODS: Weekly blood samples were obtained from 28 healthy individuals (18 females, 10 males) during 10 weeks study period...
August 20, 2018: International Journal of Laboratory Hematology
Carolyn Bunkall, Nikhil Ghallyan, Catherine Elliott, Neil Van de Water, George Chan
INTRODUCTION: Hb H inclusion test (HbH-i) commonly used for α-thalassaemia screening is not standardised and is labour-intensive. This study evaluated a strip test based on immunochromatographic detection of Hb Bart's (ICT) for use as a routine screening test for α-thalassaemia screening in the clinical laboratory setting. METHODS: The performance characteristics of the ICT was determined by comparing the results of ICT and HbH-i on 67 patients, and the α-globin genotype on 47 of these patients who also had the molecular analysis...
August 17, 2018: International Journal of Laboratory Hematology
Hugo Cruz, Ana Maria Ferreira, Emília Costa, José Barbot, Maria Inês Freitas
No abstract text is available yet for this article.
August 16, 2018: International Journal of Laboratory Hematology
Carole Nagant, Daniele Casula, Anne Janssens, Vo Thanh Phuong Nguyen, Brigitte Cantinieaux
INTRODUCTION: The discrimination of leukemia lymphoblasts (LB) in diagnosis and follow-up of B-cell progenitor acute lymphoblastic leukemia (BCP-ALL) by multiparameter flow cytometry (MFC) may be difficult due to the presence of hematogones (HG). The aim of this study was to compare lymphoblasts of BCP-ALL and HG for the expression of the most discriminating antigens. METHODS: A total of 82 bone marrow samples (39 BCP-ALL and 43 patients with HG) were analyzed using MFC...
August 16, 2018: International Journal of Laboratory Hematology
Diana M Haninger, Mehdi Nassiri, Elizabeth D Settembre, Shanxiang Zhang, Jiehao Zhou
INTRODUCTION: Peripheral blood smear (PBS) review is a routine laboratory test which requires pathologist's interpretation when abnormal indices, atypical cells, or critical findings are identified. Real-time remote digital microscopy (DM) can potentially facilitate rapid review when an on-site pathologist is not available. Herein, we assess intraobserver concordance of PBS evaluation with light microscopy (LM) and DM using VisionTek M6 robotic DM and TeamViewer imaging software. METHODS: Thirty-seven de-identified PBS slides were evaluated by five reviewers...
August 14, 2018: International Journal of Laboratory Hematology
David Barth, David Good
INTRODUCTION: No common or widely accepted criteria exist for physician review of blood films or for reported standards of physician rate of blood film review. Individual institutions generally have internal criteria for physician review of blood films. To better understand how and why blood film reviews are performed at different institutions across Canada, with a specific interest in physician blood film review, we undertook a survey to assess the current practise patterns of physician review of blood films across Canada...
July 31, 2018: International Journal of Laboratory Hematology
Hubert Lau, Alexandra Nagy, Susan K Atwater, Michael J Cascio, Robert S Ohgami
INTRODUCTION: CD13 is a myeloid associated antigen, which may be expressed by a subset of B cell lymphomas; however, the significance of its expression along with other B cell associated antigens is not well characterized. METHODS: Two hundred and eighty-six mature B cell neoplasms with flow cytometric analysis performed at the time of diagnosis were identified. Expression of CD13, CD45, CD19, CD20, CD5, CD10, CD38, CD22, CD23, FMC7, and kappa and lambda light chains was assessed for each case and correlated with clinicopathologic features...
July 31, 2018: International Journal of Laboratory Hematology
Shani Levy, Elise Schapkaitz
INTRODUCTION: Iron deficiency (ID) is a major cause of morbidity in pregnancy. Antenatal clinics use a hemoglobin to screen for ID, which delays the diagnosis of subclinical ID. The aim of this study was to investigate the clinical utility of the percentage of microcytic red cells (%Micro-R), percentage of hypochromic red cells (%Hypo-He), and reticulocyte hemoglobin content (Ret-He) on the Sysmex hematology analyzer in pregnant patients. METHODS: For this study, 102 nonanemic patients in the first trimester of pregnancy presenting for the first time to antenatal clinic were screened for ID...
July 26, 2018: International Journal of Laboratory Hematology
Steve Kitchen, Ulrich Geisen, Janos Kappelmayer, Peter Quehenberger, Anna Lowe, Robert Jones, Gabrielle Miles, Jana G Boehm, Gergely Rozsnyai
INTRODUCTION: New laboratory methods to measure haemostatic function require careful assessment before routine use. We evaluated the analytical performance of four new coagulation assays for the measurement of fibrinogen by Clauss assay, prothrombin time-derived fibrinogen, thrombin time and D-dimer levels. METHODS: The four assays were evaluated on the cobas t 711 and cobas t 511 analysers at four centres in Europe. Analytical performance and method comparisons with other commercially available assays were performed according to Clinical and Laboratory Standards Institute guidelines (EP09-A3, EP05-A3) using residual anonymized human sodium citrate (3...
July 22, 2018: International Journal of Laboratory Hematology
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