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International Journal of Laboratory Hematology

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https://www.readbyqxmd.com/read/27885817/serum-ferritin-level-is-a-prognostic-marker-in-patients-with-peripheral-t-cell-lymphoma
#1
S Koyama, S Fujisawa, R Watanabe, M Itabashi, D Ishibashi, Y Ishii, Y Hattori, Y Nakajima, K Motohashi, H Takasaki, R Kawasaki, C Hashimoto, E Yamazaki, H Koharazawa, S Takemura, N Tomita, R Sakai, S Motomura, H Nakajima
INTRODUCTION: The prognostic value of serum ferritin level in patients with peripheral T-cell lymphoma (PTCL) remains unknown. METHODS: We retrospectively analyzed clinical data from 78 consecutive patients with newly diagnosed PTCL that were treated with anthracycline-containing regimens between 1998 and 2011. RESULTS: The patients consisted of 50 males and 28 females with a median age of 64 years (range, 16-83 years). The subtypes of PTCL were 39 PTCL, not otherwise specified and 39 angioimmunoblastic T-cell lymphoma (AITL)...
November 24, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27868395/genetic-diagnosis-in-hemophilia-a-from-southern-china-five-novel-mutations-and-one-preimplantation-genetic-analysis
#2
J Chen, J Wang, X Y Lin, Y W Xu, Z H He, H Y Li, S Q Chen, W Y Jiang
INTRODUCTION: As there is currently no complete cure for hemophilia A (HA), the identification of pathogenic mutations in factor VIII (FVIII) gene from HA patients and carriers, which can contribute to genetic counseling prenatal diagnosis, and preimplantation genetic diagnosis (PGD), is an important step to prevent HA. METHODS: A total of 14 unrelated Chinese HA subjects (FVIII activity <40%), 20 carrier subjects, three fetuses, and one PGD were included in this study...
November 21, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27863067/a-rare-e9a1-bcr-abl1-fusion-transcript-in-chronic-myeloid-leukemia
#3
LETTER
Y Miao, Y Huang, C Feng, L Jiang, H Xu, Z Chen
No abstract text is available yet for this article.
November 13, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27863007/t-lymphoid-or-t-myeloid-blast-phase-of-chronic-myeloid-leukemia-in-the-era-of-tyrosine-kinase-inhibitor-therapy-a-report-of-14-cases
#4
LETTER
Z Gong, W Xie, W Wang, Z Chen, J Xu, J Yuan, Y Zhou, D Wang, L J Medeiros, S Hu
No abstract text is available yet for this article.
November 13, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27808476/no-genetic-abnormalities-identified-in-%C3%AE-2iib-and-%C3%AE-3-phenotype-overcomes-genotype-in-glanzmann-thrombasthenia
#5
LETTER
M Kannan, R Saxena
No abstract text is available yet for this article.
November 3, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27808471/neutrophil-volume-distribution-width-as-a-new-marker-in-detecting-inflammatory-bowel-disease-activation
#6
Y Aydemir, A Pınar, G Hızal, H Demir, I N Saltık Temizel, H Özen, F Akbıyık, A Yüce
INTRODUCTION: We sought to investigate the value of neutrophil volume distribution width in detecting inflammatory bowel disease activation. METHODS: Patients with infection and accompanying inflammatory disease were excluded. All the patients were diagnosed and classified according to Porto criteria and Paris classification, respectively. Physician global assessment, pediatric Crohn's disease and pediatric ulcerative colitis activity indexes and fecal calprotectin were used to define disease activation...
November 3, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27870471/analytical-evaluation-of-the-adams-%C3%A2-a1c-ha-8180-thalassemia-mode-high-pressure-liquid-chromatography-analyser-for-the-measurement-of-hba2-and-hbf
#7
E Urrechaga
BACKGROUND: ADAMS(™) A1cHA-8180T is a HPLC system; within 3.5 min, it quantifies HbF, HbA2 , and HbA0 and flags abnormal peaks. We evaluate its analytical performance for routine estimation of HbA2 and HbF, and critical tests were performed for identifying β-thalassemia carriers. METHODS: Trueness imprecision, carry over, linearity, and effect of anemia were evaluated according to ICLH, ICLS, or manufacture's guidelines. Comparison (ADAMS(™) A1c HA-8160T) was performed by running 400 samples from healthy subjects, 30 alpha and 80 beta carriers (range: 1...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27870470/list-of-reviewers
#8
(no author information available yet)
No abstract text is available yet for this article.
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27870469/a-panel-of-circulating-mirnas-as-diagnostic-biomarkers-for-screening-multiple-myeloma-a-systematic-review-and-meta-analysis
#9
REVIEW
L Zhang, D Cao, L Tang, C Sun, Y Hu
Circulating microRNAs (miRNAs) have been proved to be effective diagnostic markers for multiple myeloma (MM). The meta-analysis was aimed to evaluate the diagnostic value of related miRNAs. Multiple databases (PubMed, Web of Science, EMBASE, Cochrane Library, CBM, and CNKI) were systematically searched for available studies up to March 2016. All data were analyzed with the help of software revman 5.3 and metadisc 1.4. The eligible articles' quality was estimated by QUADAS-2, and pooled parameters were acquired with the bivariate random-effects meta-analysis model...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27574115/-complete-blood-counts-and-automated-leucocyte-differential-results-obtained-by-siemens-advia-2120i-in-145-cases-of-acute-leukaemia-in-adults-insights-into-cellular-pathophysiology-and-differential-diagnosis
#10
LETTER
https://www.readbyqxmd.com/read/27572295/spectrum-of-bone-marrow-morphologic-findings-in-hepatitis-c-patients-with-and-without-prior-liver-transplantation
#11
J M Boone, W Cui
INTRODUCTION: Cytopenia is a common hematologic finding in patients with HCV infection. Only a few studies have addressed bone marrow (BM) morphologic findings in these patients. No systemic study has been performed in these patients with liver transplantation (LT). METHODS: We retrospectively examined BMs in 49 hepatitis C patients with and without prior LT (n = 19 and n = 30). RESULTS: Among the patients with an available complete blood count (n = 46), the majority of them presented with cytopenia involving one or multiple cell lineages including unicytopenia (13%, n = 6), bicytopenia (31%, n = 14), and pancytopenia (43%, n = 20)...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27572141/validation-of-a-blood-stability-score-as-an-easy-to-use-blood-sample-quality-index
#12
N Robinson, A Kirchbichler, O Banuls, M Mader, R Aikin, P-E Sottas, G D'Onofrio
INTRODUCTION: The athlete biological passport (ABP) was implemented based on conservative requirements on sample storage and transport to ensure blood integrity. Blood remains stable over periods longer than the currently employed time limits. We investigated whether time and temperature requirements for sample storage can be used in a flexible model rather than based on fixed limits. METHODS: A literature review was performed analyzing the stability blood variables...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27571881/variability-in-platelet-dense-granule-adenosine-triphosphate-release-findings-amongst-patients-tested-multiple-times-as-part-of-an-assessment-for-a-bleeding-disorder
#13
M S Badin, L Graf, J K Iyer, K A Moffat, J L Seecharan, C P M Hayward
INTRODUCTION: Lumi-aggregometry quantification of platelet dense granule adenosine triphosphate (ATP) release is commonly used for diagnosing platelet function disorders. As the test findings show considerable variability for healthy controls, we postulated that patient findings might also be variable and investigated patients who were assessed for dense granule ATP release defects more than once. METHODS: Analyses were performed on prospectively collected data for first and second tests for subjects tested for dense granule ATP release defects more than once by the Hamilton Regional Laboratory Program (HRLMP) between January 2007 and June 2013 (cohort I)...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27565762/evaluation-of-the-performance-of-a-point-of-care-method-for-total-and-differential-white-blood-cell-count-in-clozapine-users
#14
H N Bui, J P A M Bogers, D Cohen, T Njo, M H Herruer
INTRODUCTION: We evaluated the performance of the HemoCue WBC DIFF, a point-of-care device for total and differential white cell count, primarily to test its suitability for the mandatory white blood cell monitoring in clozapine use. METHOD: Leukocyte count and 5-part differentiation was performed by the point-of-care device and by routine laboratory method in venous EDTA-blood samples from 20 clozapine users, 20 neutropenic patients, and 20 healthy volunteers. From the volunteers, also a capillary sample was drawn...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27565612/use-of-the-automated-fragmented-red-cell-count-for-monitoring-patients-with-thrombotic-thrombocytopenic-purpura
#15
LETTER
E Schapkaitz, M H Mezgebe
No abstract text is available yet for this article.
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27545696/a-variation-of-the-nijmegen-bethesda-assay-using-heat-or-a-novel-heat-cold-pretreatment-for-the-detection-of-fix-inhibitors-in-the-presence-of-residual-fix-activity
#16
A H Millner, S Tiefenbacher, M Robinson, H T Boesen
INTRODUCTION: The inclusion of a heat treatment step has improved the classic Nijmegen-Bethesda assay for detection of factor VIII (FVIII) inhibitors in the presence of residual FVIII activity (FVIII:C). However, information regarding heat-modified Nijmegen-Bethesda assays for the detection of FIX inhibitors is still limited. METHODS: Three methods to measure FIX inhibitors in the presence or absence of residual FIX activity (FIX:C) using three different activated partial thromboplastin time (aPTT) reagents were investigated...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27545617/implementing-a-routine-flow-cytometry-assay-for-nucleated-red-blood-cell-counts-in-cord-blood-units
#17
C Simard, M Cloutier, C Jobin, J Dion, D Fournier, S Néron
INTRODUCTION: As required by standards organizations, Héma-Québec Cord Blood Bank performs enumeration of nucleated red blood cells (NRBCs) in cord blood units (CBUs). This study presents the validation and implementation approaches developed to transfer the routine NRBC enumeration from the manual blood film method to a flow cytometric assay. METHODS: The flow cytometry method was adapted from Tsuji (Cytometry, 37, 1999, 291). This assay was validated to assess the specificity, detection limit, repeatability, and reproducibility of the method, including interoperator and interlaboratory testing...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27461962/delta-globin-gene-variations-leading-to-reduction-in-hba2-levels
#18
P Hariharan, S Colaco, R Colah, K Ghosh, A Nadkarni
INTRODUCTION: Mutations in the δ-globin gene are not pathogenically relevant, but co-inheritance of δ-globin variants along with β-globin gene defects can mask the diagnosis of β-thalassaemia trait. METHODS: Routine haematological parameters were carried out. Molecular analysis of β-globin gene mutations was carried out by CRDB, ARMS and DNA sequencing. δ- globin gene analysis was carried out by DNA sequencing. RESULTS: In this case study, we report a β-thalassaemia trait (IVS 1-5G→C) (HBB:c...
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27461906/development-of-a-cost-effective-duplexed-real-time-pcr-assay-for-minimal-residual-disease-monitoring-of-chronic-myeloid-leukemia-using-locked-nucleic-acid-probes
#19
LETTER
N Patkar, S Joshi, S Chaudhary, R Mascerhenas, H Doshi, P Tembhare, S Gujral, P G Subramanian
No abstract text is available yet for this article.
December 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27460189/a-novel-approach-for-bcr-abl1-standardization-to-improve-international-scale-estimation
#20
B Maes, M Bakkus, N Boeckx, E Boone, B Cauwelier, B Denys, P De Schouwer, T Devos, H El Housni, F Hillen, K Jacobs, F Lambert, H Louagie, M-B Maes, P Meeus, E Moreau, F Nollet, K Peeters, P Saussoy, P Van Lint, J-L Vaerman, F Vaeyens, K Vandepoele, P Vannuffel, K Ver Elst, K Vermeulen, R Bruyndonckx
INTRODUCTION: Standardization of BCR-ABL1 messenger RNA quantification by real-time PCR on the International Scale (IS) is critical for monitoring therapy response in chronic myelogenous leukaemia. Since 2006, BCR-ABL1 IS standardization is propagated along reference laboratories by calculating a laboratory-specific conversion factor (CF), co-ordinated in Europe through the European Treatment and Outcome Study project. Although this process has proven successful to some extent, it has not been achievable for all laboratories due to the complexity of the process and the stringent requirements in terms of numbers of samples to be exchanged...
December 2016: International Journal of Laboratory Hematology
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