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International Journal of Laboratory Hematology

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https://www.readbyqxmd.com/read/29314650/validation-of-reflex-testing-rules-and-establishment-of-a-new-workflow-for-body-fluid-cell-analysis-using-a-sysmex-xn-550-automatic-hematology-analyzer
#1
Y-U Cho, E You, S Jang, C-J Park
INTRODUCTION: We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS: We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515 samples and then validated the rules using another 507 samples (validation arm). RESULTS: All analytical performances were acceptable, and the carryover was negligible...
January 4, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29314632/comparison-of-five-specific-assays-for-determination-of-dabigatran-plasma-concentrations-in-patients-enrolled-in-the-start-laboratory-register
#2
M Cini, C Legnani, B Cosmi, S Testa, C Dellanoce, O Paoletti, R Marcucci, D Poli, R Paniccia, V Pengo, A Tripodi, G Palareti
INTRODUCTION: Several specific assays are commercially available to determine dabigatran anticoagulant activity. Aims of this multicenter and multiplatform study were to compare five methods for dabigatran measurement and investigate their performances in the low concentration range. METHODS: Dabigatran levels were analyzed in 295 plasma samples from patients enrolled in the START-Laboratory Register by the following methods using dedicated calibrators and controls: STA-ECA II (Diagnostica Stago), standard and low range Hemoclot Thrombin Inhibitors (Hyphen BioMed), Direct Thrombin Inhibitor Assay (Instrumentation Laboratory), Direct Thrombin Inhibitor Assay (Siemens), Technoclot DTI (Technoclone)...
January 3, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29274203/establishing-a-comprehensive-genetic-diagnosis-strategy-for-hemophilia-b-and-its-application-in-chinese-population
#3
X Y Lin, J Wang, X Xiao, Y W Xu, Q J Yan, W Y Jiang
INTRODUCTION: To reduce the incidence of hemophilia B (HB) which with no complete cure currently, prenatal diagnosis and preimplantation genetic diagnosis (PGD) are effective and feasible means. However, previous studies about genetic diagnosis in HB mostly just focused on the detection of patients and carriers. Here, we established a comprehensive genetic diagnosis strategy for HB and worked it out in Chinese population. The strategy includes the detection of patients and carriers, prenatal diagnosis, and PGD...
December 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29239121/effect-of-rivaroxaban-on-thrombin-generation-in-vivo-a-study-in-obese-patients
#4
LETTER
D Bertaggia-Calderara, D Kröll, C Gerschheimer, N Nicolas, P Nett, G Stirnimann, L Alberio
No abstract text is available yet for this article.
December 14, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29222848/digital-image-analysis-agrees-with-visual-estimates-of-adult-bone-marrow-trephine-biopsy-cellularity
#5
A S Hagiya, A Etman, I N Siddiqi, S Cen, G R Matcuk, R K Brynes, M E Salama
INTRODUCTION: Evaluation of cellularity is an essential component of bone marrow trephine biopsy examination. The standard practice is to report the results as visual estimates (VE). Digital image analysis (DIA) offers the promise of more objective measurements of cellularity. METHODS: Adult bone marrow trephine biopsy sections were assessed for cellularity by VE. Sections were scanned using an Aperio AT2 Scanscope and analyzed using a Cytonuclear (version 1.4) algorithm on halo software...
December 9, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29214750/comparison-of-prothrombin-time-inr-and-clot-waveform-analysis-performed-with-129-mmol-l-and-105-mmol-l-citrate-tubes
#6
LETTER
M F Ruberto, F Marongiu, D Barcellona
No abstract text is available yet for this article.
December 7, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29205868/ficoll-hypaque-separation-vs-whole-blood-lysis-comparison-of-efficiency-and-impact-on-minimal-residual-disease-analysis
#7
E Genuardi, D Barbero, I Dogliotti, B Mantoan, D Drandi, M Gambella, G M Zaccaria, L Monitillo, I Della Starza, M Cavalli, L A De Novi, E Ciabatti, S Grassi, A Gazzola, C Mannu, I Del Giudice, S Galimberti, C Agostinelli, P P Piccaluga, M Ladetto, S Ferrero
INTRODUCTION: The high-throughput era remarkably changed molecular laboratory practice. Actually, the increasing number of processed samples requires to reduce the risk of operator biases, by automating or simplifying as much as possible both the analytical and the pre-analytical phases. Minimal residual disease (MRD) studies in hematology often require a simultaneous processing of many bone marrow and peripheral blood samples from patients enrolled in prospective, multicenter, clinical trials, monitored at several planned time points...
December 4, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29160616/evaluation-of-microtitre-plate-based-haemoglobin-estimation
#8
A P Shah, P T Patel, B P Patel, K K Mishra, K Ghosh
INTRODUCTION: Haemoglobin estimation is one of the most important clinical investigations. Many techniques are available to measure haemoglobin; still there is a need for a haemoglobin assay technique which is cheap, robust and simple and can be used in field conditions very quickly using figure prick sample. We evaluated a cyanmethaemoglobin-based haemoglobin estimation using a microtitre plates for the purpose. METHODS: Microtitre plate-based haemoglobin estimation was developed using cyanmethaemoglobin-based assay and was compared with standard haematology analyser-based haemoglobin estimation in a large number of samples from a population of voluntary blood donors...
November 21, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29150911/jak2-v617f-mutation-can-be-reliably-detected-in-serum-using-droplet-digital-pcr
#9
C F Nystrand, W Ghanima, A Waage, C M Jonassen
INTRODUCTION: Detection of the JAK2 V617F mutation is a key step in the diagnosis of myeloproliferative neoplasms (MPN). Sensitive real-time quantitative PCR (qPCR) detection on peripheral blood (PB) is the most widely used method. The main objective of this study was to determine whether serum, the most common material available in archival biobanks, is a good liquid biopsy for detecting and quantifying the JAK2 V617F mutation using droplet digital PCR (ddPCR). METHODS: Paired PB and serum samples from 66 patients with MPN were used...
November 17, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29143464/mean-platelet-diameter-measurements-to-classify-inherited-thrombocytopenias
#10
K Fixter, D J Rabbolini, B Valecha, M-C Morel-Kopp, S Gabrielli, Q Chen, W S Stevenson, C M Ward
INTRODUCTION: Mean platelet volume (MPV) assists the differential diagnosis of inherited thrombocytopenia (IT) but lacks standardisation and varies between automated analysers. Classification of IT based on mean platelet diameter (MPD) has been proposed by an international collaborative study but has not been validated. METHODS: To assess the applicability of MPD to classify forms of IT, digital images of blood films from patients with established genetic causes for IT were generated, and the MPD measured (ZEISS Axio-scanner and Image J software) by a blinded reviewer...
November 16, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29134760/jak2-haplotype-46-1-and-jak2-v617f-allele-burden-in-mpn-new-evidence-against-the-hypermutability-hypothesis
#11
LETTER
M A Stolyar, O A Klimova, A S Gorbenko, E V Brenner, S E Titov, M K Ivanov, I A Olkhovskiy
No abstract text is available yet for this article.
November 14, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29115727/bleeding-score-in-type-1-von-willebrand-disease-patients-using-the-isth-bat-questionnaire
#12
A Pathare, S Al Omrani, F Al Hajri, N Al Obaidani, B Al Balushi, K Al Falahi
BACKGROUND: Bleeding assessment tools have evolved in the last decade to standardize the assessment of the severity of bleeding symptom in a consistent way. In 2010, the International Society on Thrombosis and Hemostasis-Bleeding Assessment Tool (ISTH-BAT) was developed and validated. AIMS: Our aim was to administer ISTH-BAT questionnaire to the Omani patients with type 1 VWD and obtain the bleeding score (BS). We also studied the severity of their bleeding symptoms and correlated it with the BS as well as with the laboratory parameters...
November 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29115725/comparison-of-conventional-cytomorphology-flow-cytometry-immunophenotyping-and-automated-cell-counting-of-csf-for-detection-of-cns-involvement-in-acute-lymphoblastic-leukemia
#13
J C Jaime-Pérez, M F Borrego-López, R A Jiménez-Castillo, N Méndez-Ramírez, R Salazar-Riojas, L T Fernández, D Gómez-Almaguer
BACKGROUND AND OBJECTIVE: Cytospin conventional cytomorphology (CCC) is the standard method for detecting lymphoblasts in cerebrospinal fluid (CSF) of patients with acute lymphoblastic leukemia (ALL) and for guiding treatment decisions. We evaluated flow cytometry immunophenotyping (FCI) performance for improving detection of central nervous system (CNS) involvement in ALL. METHODS: This prospective study included analysis of consecutive CSF samples of patients of all ages with ALL at 3 clinical stages: new diagnosis, relapse suspicion, and after relapse treatment...
November 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29106071/multiple-centre-evaluation-study-of-adamts13-activity-and-inhibitor-assays
#14
K Langley, R Fretwell, S Kitchen, S MacDonald, T Dutt, P Baker, D Singh, V McDonald, C Hughes, P Murphy, M Scully
INTRODUCTION: Accurate evaluation of ADAMTS13 activity is required for the diagnosis and clinical management of thrombotic microangiopathies, and commercial kits are available for routine laboratory use. METHODS: Our study compares the results from Technoclone (Technoclone GmbH, Austria) activity and Inhibitor kits with specialist laboratory reference methods (FRETS and ELISA IgG) and the impact of transporting frozen samples and comparison of results. RESULTS: This multicentre study identified differences in Technoclone activity results compared to specialist testing, which could potentially impact diagnosis...
November 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29106047/difference-in-cd5-and-cd10-expression-according-to-anatomic-site
#15
LETTER
M Sorigue, A Triguero, E Feliu, J Junca
No abstract text is available yet for this article.
November 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29090523/digital-microscopy-as-a-screening-tool-for-the-diagnosis-of-hereditary-hemolytic-anemia
#16
R Huisjes, W W van Solinge, M D Levin, R van Wijk, J A Riedl
INTRODUCTION: Evaluation of red blood cell (RBC) morphology is an important first step in the differential diagnosis of hereditary hemolytic anemia. It is, however, labor intensive, expensive, and prone to subjectivity. To improve and standardize the analysis of RBC morphology as a screening tool in the diagnosis of hereditary hemolytic anemia, we studied its automated analysis by digital microscopy (DM). METHODS: Blood from 90 patients with hereditary hemolytic anemia and 32 normal control subjects was analyzed by the CellaVision DM96 Digital Microscope...
November 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058373/impact-on-major-cbc-parameters-caused-by-different-types-of-lipids-in-patient-plasma
#17
LETTER
J Yue, X Lian, P Yue, D Xiang, C Wang
No abstract text is available yet for this article.
October 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058372/minimal-residual-disease-in-multiple-myeloma-benefits-of-flow-cytometry
#18
REVIEW
I V Galtseva, Y O Davydova, N M Kapranov, H L Julhakyan, L P Mendeleeva
Over the last 20 years, the approaches to the treatment of multiple myeloma (MM) have changed considerably, which led to an increase in remission rate. Using new diagnostic methods has made it possible to assess the response to treatment more reliably and forecast disease recurrence: allele-specific polymerase chain reaction, new-generation sequencing and multicolor flow cytometry enable minimal residual disease (MRD) detection of with sensitivity of 10-5 to 10-6 . MRD assessment with flow cytometry using is a rapidly developing area of research...
October 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29330939/corrigendum
#19
(no author information available yet)
No abstract text is available yet for this article.
February 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058381/global-assays-of-fibrinolysis
#20
LETTER
A Ilich, N S Key
No abstract text is available yet for this article.
December 2017: International Journal of Laboratory Hematology
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