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International Journal of Laboratory Hematology

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https://www.readbyqxmd.com/read/29150911/jak2-v617f-mutation-can-be-reliably-detected-in-serum-using-droplet-digital-pcr
#1
C F Nystrand, W Ghanima, A Waage, C M Jonassen
INTRODUCTION: Detection of the JAK2 V617F mutation is a key step in the diagnosis of myeloproliferative neoplasms (MPN). Sensitive real-time quantitative PCR (qPCR) detection on peripheral blood (PB) is the most widely used method. The main objective of this study was to determine whether serum, the most common material available in archival biobanks, is a good liquid biopsy for detecting and quantifying the JAK2 V617F mutation using droplet digital PCR (ddPCR). METHODS: Paired PB and serum samples from 66 patients with MPN were used...
November 17, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29143464/mean-platelet-diameter-measurements-to-classify-inherited-thrombocytopenias
#2
K Fixter, D J Rabbolini, B Valecha, M-C Morel-Kopp, S Gabrielli, Q Chen, W S Stevenson, C M Ward
INTRODUCTION: Mean platelet volume (MPV) assists the differential diagnosis of inherited thrombocytopenia (IT) but lacks standardisation and varies between automated analysers. Classification of IT based on mean platelet diameter (MPD) has been proposed by an international collaborative study but has not been validated. METHODS: To assess the applicability of MPD to classify forms of IT, digital images of blood films from patients with established genetic causes for IT were generated, and the MPD measured (ZEISS Axio-scanner and Image J software) by a blinded reviewer...
November 16, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29134760/jak2-haplotype-46-1-and-jak2-v617f-allele-burden-in-mpn-new-evidence-against-the-hypermutability-hypothesis
#3
LETTER
M A Stolyar, O A Klimova, A S Gorbenko, E V Brenner, S E Titov, M K Ivanov, I A Olkhovskiy
No abstract text is available yet for this article.
November 14, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29115727/bleeding-score-in-type-1-von-willebrand-disease-patients-using-the-isth-bat-questionnaire
#4
A Pathare, S Al Omrani, F Al Hajri, N Al Obaidani, B Al Balushi, K Al Falahi
BACKGROUND: Bleeding assessment tools have evolved in the last decade to standardize the assessment of the severity of bleeding symptom in a consistent way. In 2010, the International Society on Thrombosis and Hemostasis-Bleeding Assessment Tool (ISTH-BAT) was developed and validated. AIMS: Our aim was to administer ISTH-BAT questionnaire to the Omani patients with type 1 VWD and obtain the bleeding score (BS). We also studied the severity of their bleeding symptoms and correlated it with the BS as well as with the laboratory parameters...
November 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29115725/comparison-of-conventional-cytomorphology-flow-cytometry-immunophenotyping-and-automated-cell-counting-of-csf-for-detection-of-cns-involvement-in-acute-lymphoblastic-leukemia
#5
J C Jaime-Pérez, M F Borrego-López, R A Jiménez-Castillo, N Méndez-Ramírez, R Salazar-Riojas, L T Fernández, D Gómez-Almaguer
BACKGROUND AND OBJECTIVE: Cytospin conventional cytomorphology (CCC) is the standard method for detecting lymphoblasts in cerebrospinal fluid (CSF) of patients with acute lymphoblastic leukemia (ALL) and for guiding treatment decisions. We evaluated flow cytometry immunophenotyping (FCI) performance for improving detection of central nervous system (CNS) involvement in ALL. METHODS: This prospective study included analysis of consecutive CSF samples of patients of all ages with ALL at 3 clinical stages: new diagnosis, relapse suspicion, and after relapse treatment...
November 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29106071/multiple-centre-evaluation-study-of-adamts13-activity-and-inhibitor-assays
#6
K Langley, R Fretwell, S Kitchen, S MacDonald, T Dutt, P Baker, D Singh, V McDonald, C Hughes, P Murphy, M Scully
INTRODUCTION: Accurate evaluation of ADAMTS13 activity is required for the diagnosis and clinical management of thrombotic microangiopathies, and commercial kits are available for routine laboratory use. METHODS: Our study compares the results from Technoclone (Technoclone GmbH, Austria) activity and Inhibitor kits with specialist laboratory reference methods (FRETS and ELISA IgG) and the impact of transporting frozen samples and comparison of results. RESULTS: This multicentre study identified differences in Technoclone activity results compared to specialist testing, which could potentially impact diagnosis...
November 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29106047/difference-in-cd5-and-cd10-expression-according-to-anatomic-site
#7
LETTER
M Sorigue, A Triguero, E Feliu, J Junca
No abstract text is available yet for this article.
November 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29090523/digital-microscopy-as-a-screening-tool-for-the-diagnosis-of-hereditary-hemolytic-anemia
#8
R Huisjes, W W van Solinge, M D Levin, R van Wijk, J A Riedl
INTRODUCTION: Evaluation of red blood cell (RBC) morphology is an important first step in the differential diagnosis of hereditary hemolytic anemia. It is, however, labor intensive, expensive, and prone to subjectivity. To improve and standardize the analysis of RBC morphology as a screening tool in the diagnosis of hereditary hemolytic anemia, we studied its automated analysis by digital microscopy (DM). METHODS: Blood from 90 patients with hereditary hemolytic anemia and 32 normal control subjects was analyzed by the CellaVision DM96 Digital Microscope...
November 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058381/global-assays-of-fibrinolysis
#9
LETTER
A Ilich, N S Key
No abstract text is available yet for this article.
October 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058373/impact-on-major-cbc-parameters-caused-by-different-types-of-lipids-in-patient-plasma
#10
LETTER
J Yue, X Lian, P Yue, D Xiang, C Wang
No abstract text is available yet for this article.
October 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29058372/minimal-residual-disease-in-multiple-myeloma-benefits-of-flow-cytometry
#11
REVIEW
I V Galtseva, Y O Davydova, N M Kapranov, H L Julhakyan, L P Mendeleeva
Over the last 20 years, the approaches to the treatment of multiple myeloma (MM) have changed considerably, which led to an increase in remission rate. Using new diagnostic methods has made it possible to assess the response to treatment more reliably and forecast disease recurrence: allele-specific polymerase chain reaction, new-generation sequencing and multicolor flow cytometry enable minimal residual disease (MRD) detection of with sensitivity of 10(-5) to 10(-6) . MRD assessment with flow cytometry using is a rapidly developing area of research...
October 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29034581/a-novel-single-nucleotide-deletion-mutation-in-exon-2-hbb-c240delc-of-beta-globin-gene-a-case-report
#12
LETTER
P Tripathi, S Muthuswamy, S Agarwal
No abstract text is available yet for this article.
October 15, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29027765/factor-xiii-deficiency-diagnosis-challenges-and-tools
#13
REVIEW
M Karimi, F Peyvandi, M Naderi, A Shapiro
Factor XIII deficiency (FXIIID) is a rare hereditary bleeding disorder arising from heterogeneous mutations, which can lead to life-threatening hemorrhage. The diagnosis of FXIIID is challenging due to normal standard coagulation assays requiring specific FXIII assays for diagnosis, which is especially difficult in developing countries. This report presents an overview of FXIIID diagnosis and laboratory methods and suggests an algorithm to improve diagnostic efficiency and prevent missed or delayed FXIIID diagnosis...
October 13, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29024480/osmotic-gradient-ektacytometry-a-valuable-screening-test-for-hereditary-spherocytosis-and-other-red-blood-cell-membrane-disorders
#14
E Llaudet-Planas, J L Vives-Corrons, V Rizzuto, P Gómez-Ramírez, J Sevilla Navarro, M T Coll Sibina, M García-Bernal, A Ruiz Llobet, I Badell, P Velasco-Puyó, J L Dapena, M M Mañú-Pereira
INTRODUCTION: New generation osmotic gradient ektacytometry has become a powerful procedure for measuring red blood cell deformability and therefore for the diagnosis of red blood cell membrane disorders. In this study, we aim to provide further support to the usefulness of osmotic gradient ektacytometry for the differential diagnosis of hereditary spherocytosis by measuring the optimal cutoff values of the parameters provided by this technique. METHODS: A total of 65 cases of hereditary spherocytosis, 7 hereditary elliptocytosis, 3 hereditary xerocytosis, and 171 normal controls were analyzed with osmotic gradient ektacytometry in addition to the routine red blood cell laboratory techniques...
October 10, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28990313/in-vitro-variations-of-mean-platelet-volume-over-time-in-blood-samples-collected-in-different-anticoagulants-from-thrombocytopenic-patients-and-healthy-subjects
#15
LETTER
G M Podda, G Casazza, P Soru, M Scavone, G Vismara, M Cattaneo
No abstract text is available yet for this article.
October 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28990291/performance-evaluation-of-the-automated-nucleated-red-blood-cell-count-of-five-commercial-hematological-analyzers
#16
G Da Rin, M Vidali, F Balboni, A Benegiamo, M Borin, M L Ciardelli, F Dima, A Di Fabio, A Fanelli, F Fiorini, S Francione, L Germagnoli, M Gioia, T Lari, M Lorubbio, A Marini, A Papa, M Seghezzi, L Solarino, S Pipitone, E Tilocca, S Buoro
INTRODUCTION: Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study was to investigate the analytical performances of NRBC enumeration on five top of the range HAs. METHODS: We evaluated the within-run and between-day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE-2100 and XN-module (Sysmex), ADVIA 2120i (Siemens), BC-6800 (Mindray), and UniCel DxH 800 (Beckman Coulter)...
October 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28984423/the-triple-negative-cd34-hla-dr-cd11b-profile-rapidly-and-specifically-identifies-an-acute-promyelocytic-leukemia
#17
K Rahman, R Gupta, M K Singh, M K Sarkar, A Gupta, S Nityanand
INTRODUCTION: The genetic testing to confirm or rule out an acute promyelocytic leukemia (APL) typically takes a minimum of 24-72 hours. Flow cytometric immunophenotyping (FCI) on the other hand provides rapid and objective information to differentiate APL from non-APL. METHODS: FCI features, with single-tube 8-color combination using CD45, CD34, HAL-DR, CD11b, CD13, CD33, and CD117 and CD64, were compared for the 30 consecutive APL and 30 non-APL acute myeloid leukemia (AML) cases which morphologically mimicked an APL...
October 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28984084/relevance-of-sample-preparation-for-flow-cytometry
#18
V E Muccio, E Saraci, M Gilestro, D Oddolo, M Ruggeri, S Caltagirone, B Bruno, M Boccadoro, P Omedè
INTRODUCTION: Flow cytometry is a useful tool for diagnosis and minimal residual disease (MRD) study of hematological diseases. Standard sample preparation protocols are characterized by stain-lyse-wash (SLW). To prevent nonspecific bindings and achieve high sensitivity in MRD studies, lyse-wash-stain-wash (LWSW) is required. To our knowledge, no comparison between the two methods has been performed. METHODS: We compared mean fluorescence intensity (MFI), stain index, signal-to-noise ratio, and percentage of positive cells of 104 antibodies and of 13 selected antibodies tested in 10 samples simultaneously prepared with the two methods...
October 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28984082/performance-evaluation-of-the-microinr-%C3%A2-point-of-care-inr-testing-system
#19
J Joubert, M C van Zyl, J Raubenheimer
INTRODUCTION: Point-of-care International Normalised Ratio (INR) testing is used frequently. We evaluated the microINR(®) POC system for accuracy, precision and measurement repeatability, and investigated instrument and test chip variability and error rates. METHODS: Venous blood INRs of 210 patients on warfarin were obtained with Thromborel(®) S on the Sysmex CS-2100i(®) analyser and compared with capillary blood microINR(®) values. Precision was assessed using control materials...
October 6, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28984037/clinical-and-pathological-feature-of-bone-marrow-granulomas-a-modern-australian-series
#20
P Crispin, A Holmes
INTRODUCTION: Marrow granulomas have been commonly associated with mycobacterial infections, prompting extensive investigations in some cases where they are found, but in the setting of changing infectious epidemiology, there is a lack of recent data to guide the search for probable causes. METHODS: A retrospective case series examining the clinical and pathological findings in all cases where marrow granulomas were reported over a 10-year period at The Canberra Hospital, Australia...
October 6, 2017: International Journal of Laboratory Hematology
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