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International Journal of Laboratory Hematology

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https://www.readbyqxmd.com/read/28042696/identification-of-high-oxygen-affinity-hemoglobin-hb-andrew-minneapolis-in-an-indian-family
#1
LETTER
P Mehta, D Upadhye, P Hariharan, K Italia, P Sawant, A Nadkarni, G Subramanian, M B Mukherjee
No abstract text is available yet for this article.
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28042694/evidence-for-a-gene-conversion-in-a-hb-arya-carrier-%C3%AE-codon-47-asp-asn-hb-a1-or-hb-a2-c-142-g-a
#2
LETTER
A Pécriaux, C Paillard, A Galoisy, J Riou, N Le Metayer, H Wajcman, S Pissard
No abstract text is available yet for this article.
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28042684/detection-of-the-myd88-mutation-by-the-combination-of-the-allele-specific-pcr-and-quenching-probe-methods
#3
S Nogami, N Kawaguchi-Ihara, E Shiratori, M Ohtaka, M Itoh, S Tohda
INTRODUCTION: The MYD88 missense mutation c.794T>C, p.Leu265Pro, is found in patients with Waldenstörm's macroglobulinemia and lymphoma. Direct sequencing, allele-specific PCR (AS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), and high-resolution melting analysis (HRM) are currently used to detect the mutation; however, they are either time-consuming or have low detection sensitivity. Here, we developed a novel highly sensitive and rapid detection method based on the quenching probe (QP) technique and AS-PCR...
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28035733/frequency-of-rare-bcr-abl1-fusion-transcripts-in-chronic-myeloid-leukemia-patients
#4
A K Arun, A Senthamizhselvi, S Mani, K Vinodhini, N B Janet, K M Lakshmi, A Abraham, B George, A Srivastava, V M Srivastava, V Mathews, P Balasubramanian
INTRODUCTION: The hallmark of chronic myeloid leukemia (CML) is the presence of Philadelphia chromosome, its resultant fusion transcript (BCR-ABL1), and fusion protein (p210). Alternate breakpoints in BCR (m-bcr, μ-bcr, and others) or ABL1 result in the expression of few rare fusion transcripts (e19a2, e1a2, e13a3, e14a3) and fusion proteins (p190, p200, p225) whose exact clinical significance remains to be determined. METHODS: Our study was designed to determine the type and frequency of BCR-ABL1 fusion transcripts in 1260 CML patients and to analyze the prognosis and treatment response in patients harboring rare BCR-ABL1 fusion transcripts...
December 29, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28025866/proliferative-characteristics-of-philadelphia-negative-myeloproliferative-neoplasms-clinical-implications
#5
D Šefer, S Bižić-Radulović, N Kraguljac-Kurtović, A Bogdanović, V Čokić, P Miljić, B Beleslin-Čokić, V Knežević, O Mitrović-Ajtić, D Leković, M Gotić
INTRODUCTION: Philadelphia-negative myeloproliferative neoplasms (Ph(-) MPN) are characterized by overproduction of one or more blood cell lines. METHODS: We studied the proliferative characteristics of 91 patients with de novo Ph(-) MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34(+) cells was determined by flow cytometry...
December 26, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013530/molecular-genetic-analysis-of-ten-unrelated-iranian-patients-with-congenital-factor-xiii-deficiency
#6
LETTER
A Dorgalaleh, S Tabibian, T Bamedi, G H Tamaddon, M Naderi, B Varmaghani, H Boustani, G Dadashizadeh
No abstract text is available yet for this article.
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013529/synchronous-t-lymphoblastic-lymphoma-and-myeloid-neoplasm-with-pdgfra-rearrangement
#7
LETTER
M Pourabdollah, M Gupta, A Schimmer, H Chang
No abstract text is available yet for this article.
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013527/japanese-society-for-laboratory-hematology-flow-cytometric-reference-method-of-determining-the-differential-leukocyte-count-external-quality-assurance-using-fresh-blood-samples
#8
Y Kawai, Y Nagai, E Ogawa, H Kondo
INTRODUCTION: To provide target values for the manufacturers' survey of the Japanese Society for Laboratory Hematology (JSLH), accurate standard data from healthy volunteers were needed for the five-part differential leukocyte count. To obtain such data, JSLH required an antibody panel that achieved high specificity (particularly for mononuclear cells) using simple gating procedures. We developed a flow cytometric method for determining the differential leukocyte count (JSLH-Diff) and validated it by comparison with the flow cytometric differential leukocyte count of the International Council for Standardization in Haematology (ICSH-Diff) and the manual differential count obtained by microscopy (Manual-Diff)...
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013526/myeloperoxidase-in-blood-neutrophils-during-normal-and-abnormal-menstrual-cycles-in-women-of-reproductive-age
#9
T Shibata, J Sakamoto, Y Osaka, N Neyatani, S Fujita, Y Oka, H Takagi, H Mori, H Fujita, Y Tanaka, T Sasagawa
INTRODUCTION: We previously reported that granulocyte colony-stimulating factor (G-CSF) plays a critical role in ovulation, suggesting that neutrophils may maintain ovulation. We assessed myeloperoxidase (MPO), a major and specific enzyme of neutrophils, in women with abnormal and normal menstrual cycles to clarify the relationship between MPO and ovulation. METHODS: We analyzed MPO activity in blood neutrophils of women with abnormal menstrual cycles (indicative of anovulation, n = 12) and age- and body mass index-matched normal menstrual cycles (indicative of ovulation, n = 24) using two parameters as a marker of MPO, Neut X and mean peroxidase index (MPXI)...
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013524/evaluation-of-the-interrliner-automated-erythrocyte-sedimentation-rate-analyzer-for-a-large-academic-laboratory
#10
LETTER
E Schapkaitz, S RabuRabu
No abstract text is available yet for this article.
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004534/differential-expression-levels-and-methylation-status-of-robo1-in-mantle-cell-lymphoma-and-chronic-lymphocytic-leukaemia
#11
LETTER
A J Appe, A Aggerholm, M C Hansen, L H Ebbesen, P Hokland, H H N Bentzen, C G Nyvold
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004528/minimal-residual-disease-monitoring-in-childhood-b-lymphoblastic-leukemia-with-t-12-21-p13-q22-etv6-runx1-concordant-results-using-quantitation-of-fusion-transcript-and-flow-cytometry
#12
S J Alm, C Engvall, J Asp, L Palmqvist, J Abrahamsson, L Fogelstrand
INTRODUCTION: The translocation t(12;21)(p13;q22) resulting in the fusion gene ETV6-RUNX1, is the most frequent gene fusion in childhood B lymphoblastic leukemia. In the Nordic Society of Paediatric Haematology and Oncology ALL-2008 treatment protocol, treatment stratification in B-lineage ALL is based on results of minimal residual disease (MRD) analysis with fluorescence-activated cell sorting (FACS). In this study, we determined whether RT-qPCR of the ETV6-RUNX1 fusion transcript can be a reliable alternative for MRD analysis...
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004521/spur-cell-anemia-in-wilson-s-disease-a-rare-presentation
#13
LETTER
K Soni, M Kotru, P Dewan, P Meena
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004514/the-taipan-snake-venom-time-can-be-used-to-detect-lupus-anticoagulant-in-patients-treated-by-rivaroxaban
#14
LETTER
C Pouplard, C Vayne, C Berthomet, E A Guery, B Delahousse, Y Gruel
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27997764/a-method-comparison-study-between-two-hemoglobinometer-models-hemocue-hb-301-and-hb-201-to-measure-hemoglobin-concentrations-and-estimate-anemia-prevalence-among-women-in-preah-vihear-cambodia
#15
A I Rappaport, C D Karakochuk, K C Whitfield, K M Kheang, T J Green
INTRODUCTION: Hemoglobin (Hb) concentration is often measured in global health and nutrition surveys to determine anemia prevalence using a portable hemoglobinometer such as the Hemocue® Hb 201+. More recently, a newer model was released (Hemocue Hb 301) utilizing slightly different methods to measure Hb as compared to the older model. The objective was to measure bias and concordance between Hb concentrations using the Hemocue Hb 301 and Hb 201+ models in a rural field setting. METHODS: Hemoglobin (Hb) concentration was measured using one finger prick of blood (approximately 10 μL) from 175 Cambodian women (18-49 years) using three Hemocue Hb 201+ and three Hb 301 machines...
December 20, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27997753/characterization-of-a-novel-%C3%AE-globin-gene-cluster-deletion-causing-a-%C3%AE-%C3%AE-%C3%AE-0-thalassemia-by-next-generation-sequencing
#16
LETTER
M-Y Wu, J Li, J-M Yan, Y Zhang, D-Z Li
No abstract text is available yet for this article.
December 20, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27997752/review-of-the-nice-guidelines-for-multiple-myeloma
#17
REVIEW
G Pratt, T C Morris
In February 2016, the National Institute for Health and Care Excellence (NICE) published guidelines on multiple myeloma. NICE have published numerous guidelines relating to haematology, but this was the first guideline focusing on a single haematological malignancy. The purpose of this review was to highlight the recommendations made in the guideline and the implications for the management of patients in the UK and also internationally. In addition, we review the NICE process and highlight issues around current guideline development...
December 20, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27997738/full-blood-count-internal-qc-protocol-a-review-by-the-royal-college-of-pathologists-of-australasia-quality-assurance-programs-rcpaqap-pty-ltd-haematology
#18
J Sioufi, T Badrick, L Sinclair, K Marsden
INTRODUCTION: The RCPAQAP (Royal College of Pathologists of Australasia Quality Assurance Program) Haematology has undertaken an exercise to review the internal quality control protocol for full blood count (FBC) instrumentation as well as review the action taken by laboratories when nonconforming results are evident in the RCPAQAP proficiency testing reports. METHOD: A questionnaire was sent to laboratories enrolled in the RCPAQAP FBC module. Laboratories were asked to provide information with regard to the type of control measures used within their testing environment that would trigger alerts for nonconforming events...
December 20, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27981798/frequencies-and-phenotypic-consequences-of-association-of-%C3%AE-and-%C3%AE-thalassemia-alleles-with-sickle-cell-disease-in-bahrain
#19
S Abuamer, D K Shome, A Jaradat, A Radhi, J P Bapat, K A Sharif, J Al-Touq, A Al-Asheeri, A Al-Ajami
INTRODUCTION: Bahrain has high prevalence rates of sickle cell and thalassemia in the population. This study reports the frequencies and phenotypic characteristics of α- and/or β-thalassemia associated with sickle-cell disease (SCD) in a tertiary care hospital. METHODS: Adult SCD patients (n = 200) were screened for the common α- and β-thalassemia alleles prevalent in the region using molecular techniques. Results of CBC, hemoglobin analysis, and average annual frequencies of severe pain episodes and numbers of transfused red cell units were documented...
December 16, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27981789/comparison-of-abnormal-cell-flagging-of-the-hematology-analyzers-sysmex-xn-and-sysmex-xe-5000-in-oncohematologic-patients
#20
J R Furundarena, M Sainz, A Uranga, L Cuevas, I Lopez, J Zubicaray, A Bizjak, N Robado, M Araiz
INTRODUCTION: Hematology analyzers should optimize flagging while minimizing false-negative results and unnecessary microscopic reviews. METHODS: We compared flagging performance of Sysmex XE-5000 and XN analyzers in oncohematologic patients. Differential counts were performed by Cellavision digital system (100 cells) and a hematologist (another 100 cells). RESULTS: First, we included 292 samples (86 with blasts): 28 acute lymphoblastic leukemia, 88 acute myeloid leukemia, 91 myelodysplastic syndromes, 45 chronic myeloproliferative neoplasms, and 40 chronic myelomonocytic leukemia...
December 16, 2016: International Journal of Laboratory Hematology
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