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International Journal of Laboratory Hematology

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https://www.readbyqxmd.com/read/28133956/development-of-a-novel-automated-screening-method-for-detection-of-fviii-inhibitors
#1
M S Evans, K J Donaldson, M E Eyster
INTRODUCTION: Factor VIII activity is routinely determined by measuring the activated partial thromboplastin time (aPTT) of a patient plasma sample and determining percent activity from a standard curve. To maximize the detection of a clotting factor inhibitor, a subjective assessment of parallelism of a patient curve compared with a standard curve is performed. We developed and validated an automated objective method to assess parallelism as a rapid screening tool for detection of an inhibitor to factor VIII during routine FVIII assays...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28133951/cost-effective-approach-to-the-diagnostic-workup-of-b-cell-lymphoproliferative-disorders-via-optimal-integration-of-flow-cytometric-data
#2
E F Mason, E A Morgan, G S Pinkus, O Pozdnyakova
INTRODUCTION: The workup of lymphoproliferative disorders (LPDs) involves the combined use of flow cytometry (FC) and immunohistochemistry (IHC). This often results in duplicate immunophenotypic testing and adds costs that may not be eligible for reimbursement based on the Medicare National Correct Coding Initiative. We aimed to establish a cost-effective diagnostic algorithm based on initial FC categorization to reduce repetitive immunophenotyping. METHODS: We retrospectively reviewed 242 cases of suspected LPDs with concurrent FC and IHC testing over a 12-month period...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28133918/the-clinicopathologic-significance-of-lymphocyte-subsets-in-acute-myeloid-leukemia
#3
M Alcasid, L Ma, J R Gotlib, D A Arber, R S Ohgami
INTRODUCTION: While the role of the immune system in altering and modulating the progression of solid tumors is well studied, the impact of the immune system on the outcome and progression of hematolymphoid neoplasms is still poorly understood. METHODS: Here, we report a retrospective study detailing our analysis of 130 patients with acute myeloid leukemia (AML), with flow cytometry immunophenotypic evaluation of major lymphocyte subsets including B cells, T cells, and NK cells...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28133914/molecular-basis-of-pyruvate-kinase-deficiency-among-tunisians-description-of-new-mutations-affecting-coding-and-noncoding-regions-in-the-pklr-gene
#4
M Jaouani, L Manco, M Kalai, L Chaouch, K Douzi, A Silva, S Macedo, I Darragi, I Boudriga, D Chaouachi, Z Fitouri, R Van Wijk, M L Ribeiro, S Abbes
INTRODUCTION: Pyruvate kinase (PK) deficiency is one of the most common hereditary nonspherocytic hemolytic anemias worldwide with clinical manifestations ranging from mild to severe hemolysis. However, investigation of this enzymopathy is lacking in Tunisia. We report here a pioneer investigation of PK deficiency among Tunisian cases referred to our laboratory for biological analysis of unknown cause of hemolytic anemia. METHODS: Two hundred and fifty-three patients with unknown cause of hemolytic anemia have been addressed to our laboratory in order to investigate for red blood cells genetic disorders...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28133905/simultaneous-detection-of-45-fusion-genes-in-leukemia-by-dual-color-fluorescence-real-time-pcr
#5
Z Zheng, P Zhang, G He, K Liao, Z Wang, J Pan, K Du, J Du, B-A Li
INTRODUCTION: Detection of recurrent genetic abnormalities is of great significance for a refined diagnosis and assessment of prognosis in leukemia. Conventional nested reverse transcription PCR is labor intensive and time-consuming. METHODS: We have developed a novel dual-color TaqMan probe-based real-time PCR method for the simultaneous screening of 45 fusion transcripts in 12 parallel reactions. The method was tested and validated with cell lines carrying known fusion transcripts and patient samples...
January 30, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28120508/validation-of-automated-latex-von-willebrand-factor-activity-assay-in-a-new-prospective-study-including-post-treatment-patient-samples
#6
M Ettel, M A Nardi, L McVoy
INTRODUCTION: Von Willebrand disease (VWD) is the most prevalent inherited bleeding disorder. Diagnosis requires measurement of VWF-platelet binding function, for which VWF ristocetin cofactor activity (VWF:RCo) is the reference method. Recently, an automated latex particle-enhanced immunoturbidimetric von Willebrand factor activity assay (VWF:Ab) has been validated showing superior characteristics. We further validate VWF:Ab in a prospective study including post-treatment patient samples...
January 25, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28111935/tumor-necrosis-factor-gene-polymorphisms-in-adult-patients-with-autoimmune-hemolytic-anemia
#7
LETTER
M Pavkovic, A Petlichkovski, R Angelovic, S Genadieva-Stavric, L Cevreska, A Stojanovic
No abstract text is available yet for this article.
January 23, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28042696/identification-of-high-oxygen-affinity-hemoglobin-hb-andrew-minneapolis-in-an-indian-family
#8
LETTER
P Mehta, D Upadhye, P Hariharan, K Italia, P Sawant, A Nadkarni, G Subramanian, M B Mukherjee
No abstract text is available yet for this article.
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28042694/evidence-for-a-gene-conversion-in-a-hb-arya-carrier-%C3%AE-codon-47-asp-asn-hb-a1-or-hb-a2-c-142-g-a
#9
LETTER
A Pécriaux, C Paillard, A Galoisy, J Riou, N Le Metayer, H Wajcman, S Pissard
No abstract text is available yet for this article.
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28042684/detection-of-the-myd88-mutation-by-the-combination-of-the-allele-specific-pcr-and-quenching-probe-methods
#10
S Nogami, N Kawaguchi-Ihara, E Shiratori, M Ohtaka, M Itoh, S Tohda
INTRODUCTION: The MYD88 missense mutation c.794T>C, p.Leu265Pro, is found in patients with Waldenstörm's macroglobulinemia and lymphoma. Direct sequencing, allele-specific PCR (AS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), and high-resolution melting analysis (HRM) are currently used to detect the mutation; however, they are either time-consuming or have low detection sensitivity. Here, we developed a novel highly sensitive and rapid detection method based on the quenching probe (QP) technique and AS-PCR...
January 1, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28035733/frequency-of-rare-bcr-abl1-fusion-transcripts-in-chronic-myeloid-leukemia-patients
#11
A K Arun, A Senthamizhselvi, S Mani, K Vinodhini, N B Janet, K M Lakshmi, A Abraham, B George, A Srivastava, V M Srivastava, V Mathews, P Balasubramanian
INTRODUCTION: The hallmark of chronic myeloid leukemia (CML) is the presence of Philadelphia chromosome, its resultant fusion transcript (BCR-ABL1), and fusion protein (p210). Alternate breakpoints in BCR (m-bcr, μ-bcr, and others) or ABL1 result in the expression of few rare fusion transcripts (e19a2, e1a2, e13a3, e14a3) and fusion proteins (p190, p200, p225) whose exact clinical significance remains to be determined. METHODS: Our study was designed to determine the type and frequency of BCR-ABL1 fusion transcripts in 1260 CML patients and to analyze the prognosis and treatment response in patients harboring rare BCR-ABL1 fusion transcripts...
December 29, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013530/molecular-genetic-analysis-of-ten-unrelated-iranian-patients-with-congenital-factor-xiii-deficiency
#12
LETTER
A Dorgalaleh, S Tabibian, T Bamedi, G H Tamaddon, M Naderi, B Varmaghani, H Boustani, G Dadashizadeh
No abstract text is available yet for this article.
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013527/japanese-society-for-laboratory-hematology-flow-cytometric-reference-method-of-determining-the-differential-leukocyte-count-external-quality-assurance-using-fresh-blood-samples
#13
Y Kawai, Y Nagai, E Ogawa, H Kondo
INTRODUCTION: To provide target values for the manufacturers' survey of the Japanese Society for Laboratory Hematology (JSLH), accurate standard data from healthy volunteers were needed for the five-part differential leukocyte count. To obtain such data, JSLH required an antibody panel that achieved high specificity (particularly for mononuclear cells) using simple gating procedures. We developed a flow cytometric method for determining the differential leukocyte count (JSLH-Diff) and validated it by comparison with the flow cytometric differential leukocyte count of the International Council for Standardization in Haematology (ICSH-Diff) and the manual differential count obtained by microscopy (Manual-Diff)...
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013526/myeloperoxidase-in-blood-neutrophils-during-normal-and-abnormal-menstrual-cycles-in-women-of-reproductive-age
#14
T Shibata, J Sakamoto, Y Osaka, N Neyatani, S Fujita, Y Oka, H Takagi, H Mori, H Fujita, Y Tanaka, T Sasagawa
INTRODUCTION: We previously reported that granulocyte colony-stimulating factor (G-CSF) plays a critical role in ovulation, suggesting that neutrophils may maintain ovulation. We assessed myeloperoxidase (MPO), a major and specific enzyme of neutrophils, in women with abnormal and normal menstrual cycles to clarify the relationship between MPO and ovulation. METHODS: We analyzed MPO activity in blood neutrophils of women with abnormal menstrual cycles (indicative of anovulation, n = 12) and age- and body mass index-matched normal menstrual cycles (indicative of ovulation, n = 24) using two parameters as a marker of MPO, Neut X and mean peroxidase index (MPXI)...
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28013524/evaluation-of-the-interrliner-automated-erythrocyte-sedimentation-rate-analyzer-for-a-large-academic-laboratory
#15
LETTER
E Schapkaitz, S RabuRabu
No abstract text is available yet for this article.
December 25, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004534/differential-expression-levels-and-methylation-status-of-robo1-in-mantle-cell-lymphoma-and-chronic-lymphocytic-leukaemia
#16
LETTER
A J Appe, A Aggerholm, M C Hansen, L H Ebbesen, P Hokland, H H N Bentzen, C G Nyvold
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004528/minimal-residual-disease-monitoring-in-childhood-b-lymphoblastic-leukemia-with-t-12-21-p13-q22-etv6-runx1-concordant-results-using-quantitation-of-fusion-transcript-and-flow-cytometry
#17
S J Alm, C Engvall, J Asp, L Palmqvist, J Abrahamsson, L Fogelstrand
INTRODUCTION: The translocation t(12;21)(p13;q22) resulting in the fusion gene ETV6-RUNX1, is the most frequent gene fusion in childhood B lymphoblastic leukemia. In the Nordic Society of Paediatric Haematology and Oncology ALL-2008 treatment protocol, treatment stratification in B-lineage ALL is based on results of minimal residual disease (MRD) analysis with fluorescence-activated cell sorting (FACS). In this study, we determined whether RT-qPCR of the ETV6-RUNX1 fusion transcript can be a reliable alternative for MRD analysis...
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004521/spur-cell-anemia-in-wilson-s-disease-a-rare-presentation
#18
LETTER
K Soni, M Kotru, P Dewan, P Meena
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28004514/the-taipan-snake-venom-time-can-be-used-to-detect-lupus-anticoagulant-in-patients-treated-by-rivaroxaban
#19
LETTER
C Pouplard, C Vayne, C Berthomet, E A Guery, B Delahousse, Y Gruel
No abstract text is available yet for this article.
December 22, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28111927/management-of-concomitant-factor-vii-deficiency-and-factor-v-leiden-mutation
#20
LETTER
D Agostini-Vulaj, C W Francis, M A Refaai
No abstract text is available yet for this article.
February 2017: International Journal of Laboratory Hematology
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