journal
MENU ▼
Read by QxMD icon Read
search

Proteomics. Clinical Applications

journal
https://www.readbyqxmd.com/read/27921394/protein-biochip-based-semi-quantitative-detection-for-plasma-leptin
#1
Lei Ye, Yuan Hu, Fei-Hong Xu, Cheng-Yun Cai, Da-Wei Song, Zhen-Shan Xu, Wei-Dong Du
PURPOSE: Plasma leptin is secreted from adipose tissues and plays pivotal roles in human physiological and pathological processes. Here, we aimed at conducting a protein biochip-based sandwich-like approach for detection of plasma leptin among healthy individuals, obesity and diabetes patients. EXPERIMENTAL DESIGN: Totally, ninety-six plasma samples, including 45 healthy individuals with standard body mass index (BMI), 28 obesity and 23 diabetes patients, were recruited in the study...
December 6, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27921389/superiority-of-sds-lysis-over-saponin-lysis-for-direct-bacterial-identification-from-positive-blood-culture-bottle-by-maldi-tof-ms
#2
Yvan Caspar, Cécile Garnaud, Mariya Raykova, Sébastien Bailly, Marie Bidart, Danièle Maubon
PURPOSE: Fast species diagnosis has an important health care impact, as rapid and specific antibacterial therapy is of clear benefit for patient's outcome. Here we propose a new protocol for species identification directly from positive blood cultures. EXPERIMENTAL DESIGN: We compared four in-house protocols for bacterial identification by mass spectrometry directly from clinical positive blood cultures evaluating 2 lytic agents, SDS and saponin, and 2 protein extraction schemes, fast (FP) and long (LP)...
December 6, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27883284/novel-biomarkers-of-nasopharyngeal-carcinoma-metastasis-risk-identified-by-reverse-phase-protein-array-based-tumor-profiling-with-consideration-of-plasma-epstein-barr-virus-dna-load
#3
Tao Xu, Bojin Su, Peiyu Huang, Weihong Wei, Yanming Deng, Vasudha Sehgal, Donghui Wang, Jun Jiang, Guoyi Zhang, Anfei Li, Huiling Yang, François X Claret
PURPOSE: In patients with Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC), intertumor heterogeneity causes interpatient heterogeneity in the risk of distant metastasis. We aimed to identify novel biomarkers of metastasis risk using reverse-phase protein array (RPPA) profiling of NPC patients at risk for metastasis and considering plasma EBV DNA load. EXPERIMENTAL DESIGN: A total of 98 patients with NPC with and without metastasis after treatment, matched with respect to clinical parameters, were enrolled...
November 24, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27863071/the-putative-involvement-of-actin-binding-proteins-and-cytoskeleton-proteins-in-pathological-mechanisms-of-ketamine-cystitis-revealed-by-a-prospective-pilot-study-using-proteomic-approaches
#4
Hsueh-Hui Yang, Wei-Jun Zhai, Hann-Chorng Kuo
PURPOSE: Ketamine Cystitis (KC) among chronic ketamine young abusers has increased dramatically and it has brought attention for Urologists. The underlying pathophysiological mechanism(s) of KC is still unclear. Therefore, the purpose of this study was to elucidate the possible pathophysiological mechanism(s) of KC through proteomic techniques. EXPERIMENTAL DESIGN: Bladder tissues were obtained from 7 patients with KC, 7 patients with interstitial cystitis/bladder pain syndrome, and 5 control subjects who underwent videourodynamic study followed by augmentation enterocystoplasty to increase bladder capacity...
November 10, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27860399/toxin-a-of-the-nosocomial-pathogen-clostridium-difficile-induces-primary-effects-in-the-proteome-of-hep-2-cells
#5
Johannes Junemann, Gurbet Birgin, Jelena Erdmann, Anke Schröder, Ingo Just, Ralf Gerhard, Andreas Pich
PURPOSE: This study was carried out to investigate the impact of high concentrations of Clostridium difficile toxin A (TcdA) on the proteome of human cells. It should also be examined whether a catalytically deficient mutant (TcdANXN ) has an effect on target cells. EXPERIMENTAL DESIGN: Proteome changes were investigated after treatment of HEp-2 cells with 20 nM TcdA for 8 h using a triplex SILAC labeling method and shotgun proteomics. Proteins from differently labeled and treated cells were combined for analysis using an HPLC coupled to an Orbitrap mass spectrometer...
November 10, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27863112/label-free-quantitative-comparison-of-cerebrospinal-fluid-glycoproteins-and-endogenous-peptides-in-subjects-with-alzheimer-s-disease-mild-cognitive-impairment-and-healthy-individuals
#6
Jingxin Wang, Robert Cunningham, Henrik Zetterberg, Sanjay Asthana, Cynthia Carlsson, Ozioma Okonkwo, Lingjun Li
PURPOSE: The goal of this study is to investigate putative molecular dynamic changes in cerebrospinal fluids (CSFs) collected from individuals with mild cognitive impairment (MCI) and Alzheimer's disease (AD) as compared to healthy controls. EXPERIMENTAL DESIGN: The CSF samples from 12 subjects comprised of four cognitively normal individuals and eight patients with MCI and AD, respectively. Two aliquots of each CSF samples (total 1 mL) of each participant were used for this study...
November 9, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27863080/screening-for-potential-serum-based-proteomic-biomarkers-for-human-type-2-diabetes-mellitus-using-maldi-tof-ms
#7
Qiutao Meng, Siqi Ge, Wenhua Yan, Ruisheng Li, Jingtao Dou, Haibing Wang, Baoan Wang, Qingwei Ma, Yong Zhou, Manshu Song, Xinwei Yu, Hao Wang, Xinghua Yang, Fen Liu, Mohamed Ali Alzain, Yuxiang Yan, Ling Zhang, Lijuan Wu, Feifei Zhao, Yan He, Xiuhua Guo, Feng Chen, Weizhuo Xu, Monique Garcia, Desmond Menon, Youxin Wang, Yiming Mu, Wei Wang
BACKGROUND: Type 2 diabetes mellitus (T2DM) is a complex, pandemic disease contributing towards the global burden of health issues. To date, there are no simple clinical tests for the early detection of T2DM. METHOD: To identify potential peptide biomarkers for such applications, 406 sera of T2DM patients (n = 206) and healthy controls (n = 200) are analyzed by using MALDI-TOF MS with a cross-sectional case-control design. RESULT: Six peptides (peaks m/z 1452...
November 8, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27801555/aberrant-cytokine-secretion-and-zinc-uptake-in-chronic-cadmium-exposed-lung-epithelial-cells
#8
Yan-Ming Xu, Yang-Min Gao, Dan- Dan Wu, Fei-Yuan Yu, Zhong-Sheng Zang, Lei Yang, Yue Yao, Na-Li Cai, Yuan Zhou, Jen-Fu Chiu, Yick-Pang Ching, Andy T Y Lau
PURPOSE: Our previous results showed that cadmium (Cd)-adapted lung epithelial cells (LECs) developed resistance to apoptosis due to non-responsiveness of the c-Jun N-terminal kinase pathway and augmented expression of cytokeratin 8. Since cellular Cd entry is a prerequisite in order for Cd to elicit its cytotoxicity, therefore, we wonder if there are differential metal ion transport ability and also other phenotypic changes that occurred in these Cd-resistant LECs. EXPERIMENTAL DESIGN AND RESULTS: Here, we explored further and found that the zinc (Zn) importer Zip8 was stably abolished in these cells along with a marked decrease of Cd and Zn accumulation...
November 1, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27801551/sample-preparation-method-considerations-for-integrated-transcriptomic-and-proteomic-analysis-of-tumors
#9
Anupama Rajan Bhat, Manoj Kumar Gupta, Priya Krithivasan, Kunal Dhas, Jayalakshmi Nair, Ram Bhupal Reddy, H V Sudheendra, Sandip Chavan, Harsha Vardhan, Sujatha Darsi, Lavanya Balakrishnan, Shanmukh Katragadda, Vikram Kekatpure, Amritha Suresh, Pramila Tata, Binay Panda, Moni A Kuriakose, Ravi Sirdeshmukh
Sample processing protocols that enable compatible recovery of differentially expressed (DE) transcripts and proteins are necessary for integration of the multiomics data applied in the analysis of tumors. In this pilot study, we compared two different isolation methods for extracting RNA and protein from laryngo-pharyngeal tumor tissues and the corresponding adjacent normal sections. In Method 1, RNA and protein were isolated from a single tissue section sequentially and in Method 2, the extraction was carried out using two different sections and two independent and parallel protocols for RNA and protein...
November 1, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27774748/pretreatment-14-3-3-epsilon-level-is-predictive-for-advanced-extranodal-nk-t-cell-lymphoma-therapeutic-response-to-asparaginase-based-chemotherapy
#10
Yajuan Qiu, Zhiyuan Zhou, Zhaoming Li, Lisha Lu, Ling Li, Xin Li, Xinhua Wang, Mingzhi Zhang
PURPOSE: The aim of the present study was to identify the potential relevant biomarkers to predict the therapeutic response of advanced extranodal NK/T cell lymphoma(ENKTL) treated with asparaginase-based treatment. EXPERIMENTAL DESIGN: We used proteomic technology to identify differentially expressed proteins between chemotherapy-resistant and chemotherapy-sensitive patients. Then we used enzyme-linked immunosorbent assay to validate the predictive value of selective biomarkers...
October 24, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27763721/redox-proteomics-screening-cellular-factors-associated-with-oxidative-stress-in-hepatocarcinogenesis
#11
REVIEW
Li Zhou, Ji Wen, Zhao Huang, Edouard C Nice, Canhua Huang, Haiyuan Zhang, Qifu Li
Liver cancer is a major global health problem being the sixth most common cancer and the third cause of cancer-related death, with hepatocellular carcinoma (HCC) representing more than 90% of primary liver cancers. Mounting evidence suggests that, compared with their normal counterparts, many types of cancer cell have increased levels of ROS. Therefore, cancer cells need to combat high levels of ROS, especially at early stages of tumor development. Recent studies have revealed that ROS-mediated regulation of redox-sensitive proteins (redox sensors) is involved in the pathogenesis and/or progression of many human diseases, including cancer...
October 20, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27763719/stable-isotope-metabolic-labeling-suggests-differential-turnover-of-the-dpysl-protein-family
#12
Christoph W Turck, Christian Webhofer, Markus Nussbaumer, Larysa Teplytska, Alon Chen, Giuseppina Maccarrone, Michaela D Filiou
PURPOSE: In this work, we discuss how in vivo (15) N metabolic labeling in combination with MS simultaneously provides information on protein expression and protein turnover. EXPERIMENTAL DESIGN: We metabolically labeled mice with the stable nitrogen isotope (15) N using a (15) N-enriched diet and analyzed unlabeled ((14) N) versus (15) N-labeled brain tissue with LC-MS/MS. We then compared the (14) N versus (15) N peptide isotopologue clusters of (14) N and (15) N-labeled dihydropyrimidinase-related (DPYSL) proteins...
October 20, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27739660/the-88-kda-eales-protein-in-serum-is-a-complex-of-haptoglobin-complement-c3-and-galectin-1-as-identified-by-liquid-chromatography-coupled-mass-spectrometry
#13
Saravanan Ramanujam, Bharathselvi Muthuvel, Babu Aravind C, Jyothirmay Biswas, N Sulochana Konerirajapuram
PURPOSE: Eales' disease (ED), an enigmatic inflammatory disease, affects peripheral retinal veins and thereby vision in males. This study was aimed at identifying and deciphering the role of a novel 88-kDa protein reported in the serum and vitreous of patients with ED. EXPERIMENTAL DESIGN: The purified 88-kDa protein was identified by UPLC coupled ESI-QTOF-MS. The identified proteins were quantified in the serum from 20 ED patients and controls (age and sex matched), respectively by ELISA...
October 14, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27739254/the-caveolin-3-g56s-sequence-variant-of-unknown-significance-muscle-biopsy-findings-and-functional-cell-biological-analysis
#14
Eva Brauers, Andreas Roos, Laxmikanth Kollipara, René P Zahedi, Alf Beckmann, Nilane Mohanadas, Hartmut Bauer, Martin Häusler, Stéphanie Thoma, Wolfram Kress, Jan Senderek, Joachim Weis
In the era of next generation sequencing, we are increasingly confronted with sequence variants of unknown significance. Here, we focus on three patients carrying the sequence variant G56S in Caveolin-3 who presented with mild to moderate necrotizing myopathy and on the result of a controversial recent large scale analyses revealing that G56S is present at a frequency of around 10 % in the African population. Therefore, aim of our combined investigations was to address this discrepancy by morphological and biochemical studies...
October 14, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27739215/neuroproteomic-study-of-nitrated-proteins-in-moderate-traumatic-brain-injured-rats-treated-with-gamma-glutamyl-cysteine-ethyl-ester-administration-post-injury-insight-into-the-role-of-glutathione-elevation-in-nitrosative-stress
#15
Moses Henderson, Brittany Rice, Andrea Sebastian, Patrick G Sullivan, Christina King, Renã A S Robinson, Tanea T Reed
PURPOSE: The aims of this study are to establish a time point to determine the most beneficial time to administer GCEE post incident to reduce oxidative damage and second, by using redox proteomics, to determine if GCEE can readily suppress 3-NT modification in TBI animals. EXPERIMENTAL DESIGN: By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that the role of 3-nitrotyrosine (3-NT) formation as an intermediate will predict the involvement of protein nitration/nitrosation and oxidative damage in the brain...
October 14, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27730748/a-new-standard-of-visual-data-representation-for-imaging-mass-spectrometry-ims
#16
Matthew B O'Rourke, Matthew P Padula
PURPOSE: MALDI IMS is principally used for cancer diagnostics. In our own experience with publishing IMS data, we have been requested to modify our protocols with respect to the areas of the tissue that are imaged in order to comply with the wider literature. In light of this, we have determined that current methodologies lack effective controls and can potentially introduce bias by only imaging specific areas of the targeted tissue EXPERIMENTAL DESIGN: A previously imaged sample was selected and then cropped in different ways to show the potential effect of only imaging targeted areas...
October 11, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27672009/high-performance-mass-spectrometry-based-proteomics-reveals-enzyme-and-signaling-pathway-regulation-in-neutrophils-during-the-early-stage-of-surgical-trauma
#17
Samina Arshid, Muhammad Tahir, Belchor Fontes, Edna Frasson de Souza Montero, Mariana S Castro, Simone Sidoli, Peter Roepstorff, Wagner Fontes
PURPOSE: In clinical conditions trauma is associated with high mortality and morbidity. Neutrophils play a key role in the development of multiple organ failure after trauma EXPERIMENTAL DESIGN: To have a detailed understanding of the neutrophil activation at primary stages after trauma, neutrophils are isolated from control and surgical trauma rats in this study. Extracted proteins are analyzed using nano liquid chromatography coupled with tandem mass spectrometry. RESULTS: A total of 2924 rat neutrophil proteins are identified in our analysis, of which 393 are found differentially regulated between control and trauma groups...
September 27, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27666938/proteomic-approaches-to-quantify-cysteine-reversible-modifications-in-aging-and-neurodegenerative-diseases
#18
REVIEW
Liqing Gu, Renã A S Robinson
Cysteine is a highly reactive amino acid and is subject to a variety of reversible post-translational modifications (PTMs), including nitrosylation, glutathionylation, palmitoylation, as well as formation of sulfenic acid and disulfides. These modifications are not only involved in normal biological activities, such as enzymatic catalysis, redox signaling, and cellular homeostasis, but can also be the result of oxidative damage. Especially in aging and neurodegenerative diseases, oxidative stress leads to aberrant cysteine oxidations that affect protein structure and function leading to neurodegeneration as well as other detrimental effects...
September 26, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27666736/antibiotics-and-iron-limiting-conditions-and-their-effect-on-the-production-and-composition-of-outer-membrane-vesicles-secreted-from-clinical-isolates-of-extraintestinal-pathogenic-e-coli
#19
Kin W Chan, Clifford Shone, J Richard Hesp
PURPOSE: The focus of this study was to characterize the effect of clinically relevant stress-inducing conditions on the production and composition of proinflammatory outer membrane vesicles (OMVs) produced from ST131 extraintestinal pathogenic Escherichia coli (ExPEC) clinical isolates. EXPERIMENTAL DESIGN: A label-free method (relative normalized spectral index quantification, SINQ) was used to identify changes in the respective OMV proteomes following exposure of the ExPEC strains to antibiotics and low iron...
September 26, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27634590/proteomic-analysis-of-acetaminophen-induced-hepatotoxicity-and-identification-of-heme-oxygenase-1-as-a-potential-plasma-biomarker-of-liver-injury
#20
Yuan Gao, Zhijun Cao, Xi Yang, Mohamed A Abdelmegeed, Jinchun Sun, Si Chen, Richard D Beger, Kelly Davis, William F Salminen, Byoung-Joon Song, Donna L Mendrick, Li-Rong Yu
Overdose of acetaminophen (APAP) is a major cause of acute liver failure. To identify pathways related to hepatotoxicity and potential biomarkers of liver injury, a proteomic approach of (16) O/(18) O labeling and 2D-LC-MS/MS was used to analyze liver tissues from rats at 6 and 24 h post-treatment with low (100 mg/kg) and high (1250 mg/kg) doses of APAP. The analysis revealed that molecular pathways evolved progressively from scattered and less significant perturbations to more focused and significant alterations in a dose- and time-dependent manner...
September 16, 2016: Proteomics. Clinical Applications
journal
journal
41554
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"