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Proteomics. Clinical Applications

Xingyun Wang, Siliang Xu, Ling Chen, Dan Shen, Yan Cao, Ranran Tang, Xing Wang, Chenbo Ji, Yun Li, Xianwei Cui, Xirong Guo
PURPOSE: Peptide drugs provide promising regimes in anti-obesity treatment. We aimed to identify potential bioactive peptides involved in adipogenesis. EXPERIMENTAL DESIGN: The intracellular peptides between preadipocytes and adipocytes were compared by liquid chromatography/mass spectrometry. We attempted to evaluate the underlying biological function of the identified peptides by performing a gene ontology (GO) and pathway analysis of their precursors. To find more potential bioactive peptides, we identified 50 peptide sequences located in the functional domains with the use of the SMART and UniProt databases...
July 16, 2018: Proteomics. Clinical Applications
Minghui Zheng, Kunhua Hu, Yunwen Bao, Hongmei Lu, Jing Li, Shaowen Wu, Longqiaozi Sun, Chaohui Duan
PURPOSE: In order to study the candidate biomarkers in autoimmune cirrhosis. EXPERIMENTAL DESIGN: we first implemented Isobaric tags for relative and absolute quantitation (iTRAQ) technology on proteins prepared from serum obtained from autoimmune cirrhosis and normal controls. Proteins found to be differentially expressed were identified with liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI MS/MS) by using an Q Exactive classic ion trap mass spectrometer...
July 12, 2018: Proteomics. Clinical Applications
Mayank Saraswat, Antti Mäkitie, Tiialotta Tohmola, Amy Dickinson, Shruti Saraswat, Sakari Joenväärä, Suvi Renkonen
PURPOSE: There are no blood biomarkers to detect early-stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs. Most OSCC incidence is associated with significant morbidity and poor survival. We aimed to use mass-spectrometry (MS) technology to find specific N-glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening. EXPERIMENTAL DESIGN: Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age and sex-matched controls were collected...
July 11, 2018: Proteomics. Clinical Applications
Marcello Manfredi, Carmela Chiariello, Eleonora Conte, Annalisa Castagna, Elisa Robotti, Fabio Gosetti, Mauro Patrone, Nicola Martinelli, Antonella Bassi, Daniela Cecconi, Emilio Marengo, Oliviero Olivieri
PURPOSE: The aim of the present research was to study the plasma proteome profile of stable coronary artery disease (CAD) patients characterized by different levels of total Apo-CIII, a prognostic marker for cardiovascular risk. EXPERIMENTAL DESIGN: wo subgroups of CAD patients (n = 52) with divergent concentrations of total circulating Apo C-III (≤ and ≥ 10 mg/dL) were examined using a shotgun proteomic approach. Validation experiments were also performed with immunochemistry methods including both the patients affected by CAD (n = 119) and the subjects without CAD (CAD-free; n = 58)...
July 10, 2018: Proteomics. Clinical Applications
Tadashi Kondo
Ewing's sarcoma is an extremely rare mesenchymal malignancy of the bone, which predominantly occurs in children and young adolescents. Ewing's sarcoma is characterized by chromosomal translocations resulting in the formation of chimeric fusions between the EWS gene and transcription factors of the ETS family, such as EWS-FLI-1. The clinical outcome of Ewing's sarcoma remains poor, and novel therapeutic approaches are required. Proteomic analyses have been applied to identify the functions of the fusion gene product, and a novel mechanism of EWS-FLI-1 turnover has been proposed...
July 10, 2018: Proteomics. Clinical Applications
Manja Wölter, Charles A Okai, Derek Smith, Manuela Ruß, Werner Rath, Ulrich Pecks, Christoph H Borchers, Michael O Glocker
PURPOSE: Intrauterine growth restriction (IUGR), a major cause of fetal morbidity and mortality, is defined as a condition in which the fetus does not reach its genetically given growth potential. Screening for IUGR biomarkers in the mothers´ blood during pregnancy would be of great help for optimal pregnancy management and timing of delivery as well as for identifying fetuses requiring further surveillance during their infancies. EXPERIMENTAL DESIGN: A multiplexing serological assay based on liquid chromatography - multiple-reaction-monitoring mass spectrometry (LC-MRM/MS) was applied in a retrospective fashion for distinguishing maternal peripheral blood serum samples of pregnant women that either belonged to the IUGR group (n = 15) or to the control group (n = 15)...
June 29, 2018: Proteomics. Clinical Applications
Domenica Scumaci, Antonio Oliva, Antonio Concolino, Antonio Curcio, Claudia Vincenza Fiumara, Laura Tammè, Oscar Campuzano, Vincenzo L Pascali, Monica Coll, Anna Iglesias, Paola Berne, Gavino Casu, Erika Olivo, Pietrantonio Ricci, Ciro Indolfic, Josep Brugada, Ramon Brugada, Giovanni Cuda
PURPOSE: The Brugada syndrome (BrS) is a severe inherited cardiac disorder. Given the high genetic and phenotypic heterogeneity of this disease, we integrated in a synergic way three different "omics" approaches to elucidate the molecular mechanisms underlying the pathophysiology of BrS, as well as for identifying reliable diagnostic/prognostic markers. EXPERIMENTAL DESIGN: We performed the profiling of plasma Proteome and MiRNome in a cohort of Brugada patients that were preliminary subjected to genomic analysis to assess a peculiar gene mutation profile...
June 28, 2018: Proteomics. Clinical Applications
Christine Eymann, Gerhild Wachlin, Dirk Albrecht, Stephan Tiede, Ulrike Krummrei, Michael Jünger, Michael Hecker, Georg Daeschlein
PURPOSE: Increasing incidence of Onychomycosis and Tinea pedis in humans of industrialized countries together with deep tissue infections are a therapeutic challenge in clinical mycology. For a better understanding of the pathology and immunology of infection we analyzed the exoproteomes of three reference strains of the most common clinical dermatophyte species (Trichophyton rubrum, T. interdigitale, A. benhamiae) and of Trichophyton strains isolated from affected patients. EXPERIMENTAL DESIGN: Extracellular proteins of those in-vitro grown strains were separated via 2D High Performance Electrophoresis and identified by mass spectrometry to find proteins with provoked host immune reactivity...
June 28, 2018: Proteomics. Clinical Applications
Kwang Hoe Kim, Soo-Youn Lee, Heeyoun Hwang, Ju Yeon Lee, Eun Sun Ji, Hyun Joo An, Jin Young Kim, Jong Shin Yoo
PURPOSE: Alpha-fetoprotein (AFP) is a widely used serological marker that is associated with hepatocellular carcinoma (HCC). Although the level of AFP is increased in HCC, its sensitivity for diagnosis is poor because AFP levels are also increased in liver diseases. Changes in glycoform, especially fucosylation, have been reported to be associated with the development of HCC. EXPERIMENTAL DESIGN: The authors introduce the monitoring of fucosylated glycopeptides by liquid chromatography (LC)-mass spectrometry (MS) combined with immunoprecipitation, where glycan-cleaved fragments with an amino acid sequence are used as transitions...
June 11, 2018: Proteomics. Clinical Applications
Kiyonaga Fujii, Yuka Miyata, Ikuya Takahashi, Hirotaka Koizumi, Hisashi Saji, Masahiro Hoshikawa, Masayuki Takagi, Toshihide Nishimura, Haruhiko Nakamura
PURPOSE: The molecular underpinnings that may prognosticate survival and increase our understanding of tumor development and progression are still poorly understood. This study aimed to define the molecular signatures for malignancy in small cell lung carcinoma (SCLC), which is known for its highly aggressive clinical features and poor prognosis. EXPERIMENTAL DESIGN: Using clinical specimens, the authors perform a comparative proteomic analysis of high-grade SCLCs and low-grade pulmonary carcinoid tumors (PCTs), both of which are types of neuroendocrine tumors...
June 11, 2018: Proteomics. Clinical Applications
Benjamin A Katchman, Meilin Zhu, Jennifer Blain Christen, Karen S Anderson
PURPOSE: Sweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling of immune-related biomarkers from healthy individuals are presented. EXPERIMENTAL DESIGN: Eccrine sweat samples are collected from 50 healthy individuals. LC-MS/MS is performed on two pools of sweat samples from five male and female participants. Individual sweat samples are analyzed by antibody isotyping microarrays (n = 49), human cytokine arrays (n = 30), and quantitative ELISAs for interleukin-1α (n = 16), epidermal growth factor (n = 6), and angiogenin (n = 7)...
June 8, 2018: Proteomics. Clinical Applications
Maurizio Ronci, Lidia Leporini, Paolo Felaco, Vittorio Sirolli, Luisa Pieroni, Viviana Greco, Antonio Aceto, Andrea Urbani, Mario Bonomini
PURPOSE: The artificial membrane inside the haemodialyzer is the main determinant of the quality and success of haemodialysis therapy. The performances of haemodialysis membranes are highly influenced by the interactions with plasma proteins, which in turn are related to the physical and chemical characteristics of the membrane material. The present cross-over study is aimed to analyse the haemodialysis performance of a newly developed asymmetric cellulose triacetate membrane (ATA) in comparison to the conventional parent symmetric polymer (CTA)...
May 28, 2018: Proteomics. Clinical Applications
Lin Yang, Zhou Yang, Lei Cheng, Juan Cheng, Li Cheng, Yangyang Sun, Wenting Li, Kai Song, Weichun Huang, Yong Yin, Shengce Tao, Qinghua Zhang
PURPOSE: Etiological diagnosis of pediatric patients with community-acquired pneumonia is difficult. For therapy, one of the major problems is the difficulty in separating bacterial pneumonia which would benefit from antibiotics from nonbacterial pneumonia. Therefore, to identify potential biomarkers for distinguishing nonbacterial pneumonia from bacterial pneumonia are sought . EXPERIMENTAL DESIGN: Lectin microarray containing 91 lectins is used to screen serums from pediatric patients with pneumonia...
May 22, 2018: Proteomics. Clinical Applications
Yang Liu, Lu Feng, Hua Wang, Yu-Jia Wang, Hsiao-Chang Chan, Xiao-Hua Jiang, Wei-Ming Fu, Gang Li, Jin-Fang Zhang
PURPOSE: Our previous study reported an elevated inflammation during tendon injury in mice with cystic fibrosis (CF), indicating the inadequate management of inflammation due to dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR). The objective of this study is to identify the targets of CFTR that contribute to the abnormal inflammation during tendon injury. EXPERIMENTAL DESIGN: A two-dimensional gel electrophoresis and mass spectrometry-based comparative proteomics was performed to find the molecular targets of CFTR...
May 21, 2018: Proteomics. Clinical Applications
Mindi Zhao, Yehong Yang, Zhengguang Guo, Chen Shao, Haidan Sun, Yang Zhang, Ying Sun, Yaoran Liu, Yijun Song, Liwei Zhang, Qian Li, Juntao Liu, Mingxi Li, Youhe Gao, Wei Sun
PURPOSE: Body fluid is considered a rich source of disease biomarkers. Proteins in many body fluids have potential clinical applications for disease diagnostic and prognostic predictions. EXPERIMENTAL DESIGN: To determine differences in the protein components and functional features of body fluids, a proteomic comparison of five body fluids (plasma, urine, cerebrospinal fluid, saliva, and amniotic fluid) was conducted by high-resolution mass spectrometry. RESULTS: A total of 4717 nonredundant proteins were identified, and the concentrations of 3433 proteins were estimated by an intensity-based algorithm quantitation method...
May 21, 2018: Proteomics. Clinical Applications
Juan Yang, Yanni Chen, Xiaofan Xiong, Xiaobo Zhou, Lin Han, Lei Ni, Wenjing Wang, Xiaofei Wang, Lingyu Zhao, Dongdong Shao, Chen Huang
PURPOSE: Autism spectrum disorder (ASD) is a neurological and developmental disorder that begins early in childhood and lasts throughout one's life. Early diagnosis is essential for ASD since early treatment can enable children with ASD to make significant gains in language and social skills, but remains challenging since there are currently no specific biomarkers of ASD. This study is aimed to identify serum biomarkers for ASD. EXPERIMENTAL DESIGN: Serum of Han Chinese children with ASD (n = 68) and age-matched healthy controls (n = 80) is analyzed using magnetic bead-based separation combined with mass spectrum...
May 13, 2018: Proteomics. Clinical Applications
Oliver Klein, Thorsten Hanke, Grit Nebrich, Junfeng Yan, Benedikt Schubert, Patrick Giavalisco, Frank Noack, Herbert Thiele, Salah A Mohamed
PURPOSE: Atrial fibrillation (AF) is a cardiac arrhythmia characterized by a rapid and irregular heart rhythm. AF types, paroxysmal (PX), persistent (PE), and long-lasting persistent (LSP), require differences in clinical management. Unfortunately, a significant proportion of AF patients are clinically misclassified. Therefore, the aim of this study is to prove that MALDI-Imaging (IMS) is valuable as a diagnostic aid in AF subtypes' assessment. EXPERIMENTAL DESIGN: Patients are clinically classified according to the guidelines of the European Society of Cardiology...
May 13, 2018: Proteomics. Clinical Applications
Ravikanth Danda, Kalaivani Ganapathy, Gajanan Sathe, Anil K Madugundu, Uma Maheswari Krishnan, Vikas Khetan, Pukhraj Rishi, Harsha Gowda, Akhilesh Pandey, Krishnakumar Subramanian, T S Keshava Prasad, Sailaja V Elchuri
PURPOSE: Retinoblastoma (RB) is a pediatric ocular cancer which is caused due to the aberrations in the RB1 gene. The changes in the membrane proteomics would help in understanding the development of the retinoblastoma and could identify candidates for biomarkers and therapy. EXPERIMENTAL DESIGN: Quantitative proteomics is performed on the enriched membrane fractions from pooled normal retina (n = 5) and pooled retinoblastoma tissues (n = 5). The proteins are tryptic-digested and tagged with iTRAQ labels...
May 9, 2018: Proteomics. Clinical Applications
Hong Qiu, Songshan Shi, Shunchun Wang, Hong Peng, Shi-Jian Ding, Lianchun Wang
PURPOSE: Vascular smooth muscle cells (VSMC) and endothelial cells (EC) communicate mutually to coordinate vascular development and homeostasis. Exosomes are emerging as one type of the mediators involved in this communication. Characterizing proteins in the exosomes is the critical first step in understanding how the VSMC-EC crosstalk is mediated by exosomes. EXPERIMENTAL DESIGN: The proteins in the human VSMC-derived exosomes are profiled using nanoLC-MS/MS based proteomics...
April 24, 2018: Proteomics. Clinical Applications
Sanjeeva Srivastava
No abstract text is available yet for this article.
July 2018: Proteomics. Clinical Applications
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