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Q L Pham, N A N Tong, A Mathew, S Basuray, R S Voronov
A custom-built mask aligner (CBMA), which fundamentally covers all the key features of a commercial mask aligner, while being low cost and light weight and having low power consumption and high accuracy, is constructed. The CBMA is composed of a custom high fidelity light emitting diode light source, a vacuum chuck, a mask holder, high-precision translation and rotation stages, and high resolution digital microscopes. The total cost of the system is under $7500, which is over ten times cheaper than a comparable commercial system...
July 2018: Biomicrofluidics
Maciej Urbaniak, Dennis Holzinger, Arno Ehresmann, Feliks Stobiecki
Colloidal magnetophoretic lensing of water suspended micrometer-sized superparamagnetic beads (SPBs) above a topographically patterned magnetic thin film system with perpendicular magnetic anisotropy is demonstrated. The magnetic pattern consisting of concentric annuli of micron-sized widths has been superimposed with a rotating external magnetic field, and it is shown that the trajectories of the SPBs above this structure are similar to light rays in an optical focusing lens. SPB trajectories converge towards the central region and have divergent trajectories while passing the center...
July 2018: Biomicrofluidics
Ishita Biswas, Mohtada Sadrzadeh, Aloke Kumar
We investigate the effect of biofouling in a microfluidic filtration system. The microfluidic platform consists of cylindrical microposts with a pore-spacing of 2  μ m, which act as the filtration section of the device. One of our key findings is that there exists a critical pressure difference above which pronounced streamer formation is observed, which eventually leads to rapid clogging of the device with an accompanying exponential decrease in permeate flow. Moreover, when streamers do form, de-clogging of pores also occurs intermittently, which leads to small time scale fluctuations [O(101  s)] superimposed upon the large time scale [O(102  min)] clogging of the system...
July 2018: Biomicrofluidics
Ngan Nguyen, Peter Thurgood, Jiu Yang Zhu, Elena Pirogova, Sara Baratchi, Khashayar Khoshmanesh
Here, we describe a novel method for fabrication of microfluidic structures in classroom environments. This method is based on replica moulding of pasta structures in polydimethylsiloxane. Placing pasta structures on a petroleum jelly base layer enables templating round-shaped structures with controllable cross-sectional profiles. The pasta structures can be easily deformed and combined to create more complex 3D microfluidic structures. Proof-of-concept experiments indicate the capability of this method for studying the mixing of neighbouring flows, generation of droplets, lateral migration of particles, as well as culturing, shear stress stimulation, and imaging of cells...
July 2018: Biomicrofluidics
P Madadkar, P R Selvaganapathy, R Ghosh
PEGylation is increasingly being utilized to enhance the therapeutic efficacy of biopharmaceuticals. Various chemistries and reaction conditions have been established to synthesize PEGylated proteins and more are being developed. Both the extent of conversion and selectivity of protein PEGylation are highly sensitive to process variables and parameters. Therefore, microfluidic-based high-throughput screening platforms would be highly suitable for optimization of protein PEGylation. As part of this study, a poly-dimethylsiloxane-based continuous flow microreactor system was designed and its performance was compared head-to-head with a batch reactor...
July 2018: Biomicrofluidics
Zehao Pan, Yongfan Men, Satyajyoti Senapati, Hsueh-Chia Chang
We report a new immersed alternating current (AC) electrospray droplet generation method that can generate monodispersed water-in-oil droplets, with diameters ranging from 5  μ m to 150  μ m, in a stationary oil phase. This method offers high through-put, easy size tuning, and droplets with a viscous aqueous phase at high ionic strengths (raw physiological samples). Yet, it does not require coordinated flows of the dispersed/continuous phases or even a microfluidic chip. The design relies on a small constant back pressure (less than 0...
July 2018: Biomicrofluidics
Ya Liu, Jiyu Li, Dinglong Hu, Josh H M Lam, Dong Sun, Stella W Pang, Raymond H W Lam
Functional microbeads have been widely applied in molecular identification and other biochemical applications in the past decade, owing to the compatibility with flow cytometry and the commercially available microbeads for a wide range of molecular identification. Nevertheless, there is still a technical hurdle caused by the significant sample volume required (∼50  μ l), limited molecular detection limit (∼20 pg/ml), complicated liquid/microbead handling procedures, and the long reaction time (>2 h)...
July 2018: Biomicrofluidics
Netanel Korin, Josué Sznitman
No abstract text is available yet for this article.
July 2018: Biomicrofluidics
Krzysztof Langer, Nicolas Bremond, Laurent Boitard, Jean Baudry, Jérôme Bibette
[This corrects the article DOI: 10.1063/1.5037795.].
July 2018: Biomicrofluidics
Rhys Cartlidge, Donald Wlodkowic
Quantification of neuro-behavioural responses of intact small model organisms has been proposed as a sensitive, sub-lethal alternative to conventional toxicity testing. Such bioassays are characterized by a high physiological and ecological relevance, short response times, increased sensitivity, and non-invasive nature. Despite a significant potential for predictive aquatic toxicology analysis of behavioural traits of micro-invertebrates in microfluidic environment has received little attention. In this work, we demonstrate a new Lab-on-a-Chip technology capable of effectively caging freshwater rotifers Brachionus calyciflorus for real-time video-microscopy analysis...
July 2018: Biomicrofluidics
Chang Hyun Cho, Seyong Kwon, Segi Kim, Yoonmi Hong, Pilnam Kim, Eun Sook Lee, Je-Kyun Park
We present here a novel microfluidic platform that can perform microfluidic on-chip immunohistochemistry (IHC) processes on a formalin-fixed paraffin-embedded section slide. Unlike previous microfluidic IHC studies, our microfluidic chip made of organic solvent-resistant polyurethane acrylate (PUA) is capable of conducting on-chip IHC processes consecutively. A narrow channel wall structure of the PUA chip shows effective sealing by pressure-based reversible assembly with a section slide. We performed both on-chip IHC and conventional IHC processes and compared the IHC results based on the immunostaining intensity...
July 2018: Biomicrofluidics
Toshio Takayama, Hiroki Miyashiro, Chia-Hung Dylan Tsai, Hiroaki Ito, Makoto Kaneko
This paper proposes an on-chip density mixer that can achieve even density in a target chamber with a swirling flow enhanced by an air chamber. The system is composed of a main channel, a target chamber where two liquids with different densities are included, an isolated air chamber, and an external vibration pump driven by a piezo actuator at the entrance of the main channel. The air chamber is expected to amplify the vibration owing to structure softening. The amplification would be more pronounced at the resonance frequencies of the structure...
July 2018: Biomicrofluidics
Saeed Rismani Yazdi, Reza Nosrati, Corey A Stevens, David Vogel, Carlos Escobedo
[This corrects the article DOI: 10.1063/1.5024508.].
July 2018: Biomicrofluidics
Hiroshi Kimura, Masaki Nishikawa, Naomi Yanagawa, Hiroko Nakamura, Shunsuke Miyamoto, Morgan Hamon, Peter Hauser, Lifu Zhao, Oak D Jo, Mitsuru Komeya, Takehiko Ogawa, Norimoto Yanagawa
Most kidney cells are continuously exposed to fluid shear stress (FSS) from either blood flow or urine flow. Recent studies suggest that changes in FSS could contribute to the function and injury of these kidney cells. However, it is unclear whether FSS influences kidney development when urinary flow starts in the embryonic kidneys. In this study, we evaluated the influence of FSS on in vitro cultured ureteric bud (UB) cells by using a pumpless microfluidic device, which offers the convenience of conducting parallel cell culture experiments while also eliminating the need for cumbersome electronic driven equipment and intricate techniques...
July 2018: Biomicrofluidics
Krzysztof Langer, Nicolas Bremond, Laurent Boitard, Jean Baudry, Jérôme Bibette
Droplet-based microfluidics, using water-in-oil emulsion droplets as micro-reactors, is becoming a widespread method for performing assays and especially in the cell biology field. Making a simple and highly portable system for creating emulsion droplets would help to continue the popularization of such a technique. Also, the ability to emulsify all the samples would strengthen this compartimenlization technique to handle samples with limited volume. Here, we propose a strategy of droplet formation that combines a classical flow-focusing microfluidic chip, which could be commercially available, with a standard laboratory adjustable micropipette...
July 2018: Biomicrofluidics
Elzbieta Jastrzebska, Agnieszka Zuchowska, Sylwia Flis, Patrycja Sokolowska, Magdalena Bulka, Artur Dybko, Zbigniew Brzozka
Poly(dimethylsiloxane) (PDMS) is a material applicable for tissue and biomedical engineering, especially based on microfluidic devices. PDMS is a material used in studies aimed at understanding cell behavior and analyzing the cell adhesion mechanism. In this work, biological characterization of the modified PDMS surfaces based on cell attachment and toxicity assays was performed. We studied Balb 3T3/c, HMEC-1, and HT-29 cell adhesion on poly(dimethylsiloxane) surfaces modified by different proteins, with and without pre-activation with plasma oxygen and UV irradiation...
July 2018: Biomicrofluidics
Paul M Holloway, Jonathan Butement, Manjunath Hegde, Jonathan West
In this contribution, a channel aspect ratio of >2 was used to access high velocity regimes to provide confined sample cores by Dean focussing in advance of linear inertial focussing. This produces a singular separation origin with a mirrored transport path for efficient particle and blood cell sorting, while also increasing the spatial resolution for multiscale sorting.
July 2018: Biomicrofluidics
Shigeru Tada, Yui Omi, Masanori Eguchi
Various microfluidic devices utilizing the principle of dielectrophoresis (DEP) have been developed to separate, concentrate, and characterize biological cells; however, their performance is still limited by a lack of quantitative characterization. We addressed this limitation by employing a method capable of accurately quantifying a cell's response to an imposed field gradient. In this study, a simple method using a newly designed Creek-gap electrode was proposed, and the electrokinetic behavior of cells was characterized by DEP velocimetry under the exposure of an approximately constant gradient of electric field square established along the gap of the electrodes...
July 2018: Biomicrofluidics
Ming-Jie Lin, Yen-Fu Liu, Ching-Chou Wu
Immunoreaction of specific antibodies to antigens is widely used in numerous immunoanalysis applications. However, diffusion-dominated transport in stationary solutions limits the rate and binding density of immunoreaction. This research describes the construction of chip-type concentric multi-double ring electrodes and single central disk electrode. A +1 V-biased 6 Vpp voltage was applied to the multi-double ring electrodes to induce a long-range DC-biased AC electrokinetic flow (ACEKF). The immunoreaction was quantified by electrochemical impedance spectrum (EIS)...
July 2018: Biomicrofluidics
Yusuf B Arık, Marinke W van der Helm, Mathieu Odijk, Loes I Segerink, Robert Passier, Albert van den Berg, Andries D van der Meer
Disruption of tissue barriers formed by cells is an integral part of the pathophysiology of many diseases. Therefore, a thorough understanding of tissue barrier function is essential when studying the causes and mechanisms of disease as well as when developing novel treatments. In vitro methods play an integral role in understanding tissue barrier function, and several techniques have been developed in order to evaluate barrier integrity of cultured cell layers, from microscopy imaging of cell-cell adhesion proteins to measuring ionic currents, to flux of water or transport of molecules across cellular barriers...
July 2018: Biomicrofluidics
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