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Biomicrofluidics

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https://www.readbyqxmd.com/read/28611871/efficient-extraction-of-oil-from-droplet-microfluidic-emulsions
#1
J R Haliburton, S C Kim, I C Clark, R A Sperling, D A Weitz, A R Abate
Droplet microfluidic techniques can perform large numbers of single molecule and cell reactions but often require controlled, periodic flow to merge, split, and sort droplets. Here, we describe a simple method to convert aperiodic flows into periodic ones. Using an oil extraction module, we efficiently remove oil from emulsions to readjust the droplet volume fraction, velocity, and packing, producing periodic flows. The extractor acts as a universal adaptor to connect microfluidic modules that do not operate under identical flow conditions, such as droplet generators, incubators, and merger devices...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28611870/acetylated-bovine-serum-albumin-differentially-inhibits-polymerase-chain-reaction-in-microdevices
#2
Naveen Ramalingam, Majid Ebrahimi Warkiani, Thomas Hai-Qing Gong
Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28611869/automatic-optimization-of-drug-cocktails-on-an-integrated-microfluidic-system
#3
Wen-Yen Huang, Ching-An Liu, Rong-Syuan Fan, Zhi-Da Lin, Kuan Wang, Gwo-Bin Lee
Drug cocktails have been popular for a variety of therapies of complicated diseases. Nevertheless, it is a tediously challenging task to optimize formulations, especially using traditional methods. Hence, an automatic system capable of precise dispensing multiple drugs is of great need. Herein, a new integrated microfluidic system combined with a two-axis traverse module was developed to dispense and mix a small amount of drug combination precisely and automatically. This on-chip dispensing process could be performed with a precise and accurate manner when compared to the manual operations...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28580046/characterization-of-microfluidic-clamps-for-immobilizing-and-imaging-of-drosophila-melanogaster-larva-s-central-nervous-system
#4
Reza Ghaemi, Pouya Rezai, Fatemeh Rafiei Nejad, Ponnambalam Ravi Selvaganapathy
Drosophila melanogaster is a well-established model organism to understand biological processes and study human diseases at the molecular-genetic level. The central nervous system (CNS) of Drosophila larvae is widely used as a model to study neuron development and network formation. This has been achieved by using various genetic manipulation tools such as microinjection to knock down certain genes or over-express proteins for visualizing the cellular activities. However, visualization of an intact-live neuronal response in larva's Central Nervous System (CNS) is challenging due to robust digging/burrowing behaviour that impedes neuroimaging...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28580045/evaluation-and-comparison-of-two-microfluidic-size-separation-strategies-for-vesicle-suspensions
#5
Kari J Storslett, Susan J Muller
Two size-based separation strategies are evaluated for suspensions consisting of giant unilamellar vesicles with a broad, continuous distribution of diameters. Microfluidic devices were designed to separate an initial suspension into larger and smaller particles via either filtration or inertial focusing. These separation mechanisms were tested with suspensions of vesicles and suspensions of rigid spheres separately to illustrate the effect of deformability on separation ability. We define several separation metrics to assess the separation ability and to enable comparison between separation strategies...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28580044/an-intra-droplet-particle-switch-for-droplet-microfluidics-using-bulk-acoustic-waves
#6
Anna Fornell, Mathias Ohlin, Fabio Garofalo, Johan Nilsson, Maria Tenje
To transfer cell- and bead-assays into droplet-based platforms typically requires the use of complex microfluidic circuits, which calls for methods to switch the direction of the encapsulated particles. We present a microfluidic chip where the combination of acoustic manipulation at two different harmonics and a trident-shaped droplet-splitter enables direction-switching of microbeads and yeast cells in droplet microfluidic circuits. At the first harmonic, the encapsulated particles exit the splitter in the center daughter droplets, while at the second harmonic, the particles exit in the side daughter droplets...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529674/erratum-publisher-s-note-maximizing-particle-concentration-in-deterministic-lateral-displacement-arrays-biomicrofluidics-11-024121-2017
#7
Shilun Feng, Alison M Skelley, Ayad G Anwer, Guozhen Liu, David W Inglis
[This corrects the article DOI: 10.1063/1.4981014.].
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529673/on-chip-two-photon-metabolic-imaging-for-drug-toxicity-testing
#8
Fang Yu, Shuangmu Zhuo, Yinghua Qu, Deepak Choudhury, Zhiping Wang, Ciprian Iliescu, Hanry Yu
We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529672/on-chip-density-based-purification-of-liposomes
#9
Siddharth Deshpande, Anthony Birnie, Cees Dekker
Due to their cell membrane-mimicking properties, liposomes have served as a versatile research tool in science, from membrane biophysics and drug delivery systems to bottom-up synthetic cells. We recently reported a novel microfluidic method, Octanol-assisted Liposome Assembly (OLA), to form cell-sized, monodisperse, unilamellar liposomes with excellent encapsulation efficiency. Although OLA provides crucial advantages over alternative methods, it suffers from the presence of 1-octanol droplets, an inevitable by-product of the production process...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529671/the-advection-of-microparticles-mcf-7-and-mda-mb-231-breast-cancer-cells-in-response-to-very-low-reynolds-numbers
#10
Sinéad T Morley, Michael T Walsh, David T Newport
The lymphatic system is an extensive vascular network that serves as the primary route for the metastatic spread of breast cancer cells (BCCs). The dynamics by which BCCs travel in the lymphatics to distant sites, and eventually establish metastatic tumors, remain poorly understood. Particle tracking techniques were employed to analyze the behavior of MCF-7 and MDA-MB-231 BCCs which were exposed to lymphatic flow conditions in a 100 μm square microchannel. The behavior of the BCCs was compared to rigid particles of various diameters (η = dp/H= 0...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529670/perspective-fabrication-of-integrated-organ-on-a-chip-via-bioprinting
#11
Qingzhen Yang, Qin Lian, Feng Xu
Organ-on-a-chip has emerged as a powerful platform with widespread applications in biomedical engineering, such as pathology studies and drug screening. However, the fabrication of organ-on-a-chip is still a challenging task due to its complexity. For an integrated organ-on-a-chip, it may contain four key elements, i.e., a microfluidic chip, live cells/microtissues that are cultured in this chip, components for stimulus loading to mature the microtissues, and sensors for results readout. Recently, bioprinting has been used for fabricating organ-on-a-chip as it enables the printing of multiple materials, including biocompatible materials and even live cells in a programmable manner with a high spatial resolution...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503248/stable-microfluidic-flow-focusing-using-hydrostatics
#12
Vaskar Gnyawali, Mohammadali Saremi, Michael C Kolios, Scott S H Tsai
We present a simple technique to generate stable hydrodynamically focused flows by driving the flow with hydrostatic pressure from liquid columns connected to the inlets of a microfluidic device. Importantly, we compare the focused flows generated by hydrostatic pressure and classical syringe pump driven flows and find that the stability of the hydrostatic pressure driven technique is significantly better than the stability achieved via syringe pumps, providing fluctuation-free focused flows that are suitable for sensitive microfluidic flow cytometry applications...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503247/reconfigurable-microfluidic-device-with-discretized-sidewall
#13
Masahiro Oono, Keisuke Yamaguchi, Amirul Rasyid, Atsushi Takano, Masato Tanaka, Nobuyuki Futai
Various microfluidic features, such as traps, have been used to manipulate flows, cells, and other particles within microfluidic systems. However, these features often become undesirable in subsequent steps requiring different fluidic configurations. To meet the changing needs of various microfluidic configurations, we developed a reconfigurable microfluidic channel with movable sidewalls using mechanically discretized sidewalls of laterally aligned rectangular pins. The user can deform the channel sidewall at any time after fabrication by sliding the pins...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503246/particle-concentrating-and-sorting-under-a-rotating-electric-field-by-direct-optical-liquid-heating-in-a-microfluidics-chip
#14
Yu-Liang Chen, Hong-Ren Jiang
We demonstrate a functional rotating electrothermal technique for rapidly concentrating and sorting a large number of particles on a microchip by the combination of particle dielectrophoresis (DEP) and inward rotating electrothermal (RET) flows. Different kinds of particles can be attracted (positive DEP) to or repelled (negative DEP) from electrode edges, and then the n-DEP responsive particles are further concentrated in the heated region by RET flows. The RET flows arise from the spatial inhomogeneous electric properties of fluid caused by direct infrared laser (1470 nm) heating of solution in a rotating electric field...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503245/maximizing-particle-concentration-in-deterministic-lateral-displacement-arrays
#15
Shilun L Feng, Alison M Skelley, Ayad G Anwer, Guozhen Liu, David W Inglis
We present an improvement to deterministic lateral displacement arrays, which allows higher particle concentration enhancement. We correct and extend previous equations to a mirror-symmetric boundary. This approach allows particles to be concentrated into a central channel, no wider than the surrounding gaps, thereby maximizing the particle enrichment. The resulting flow patterns were, for the first time, experimentally measured. The performance of the device with hard micro-spheres and cells was investigated...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28469763/flow-lithography-in-ultraviolet-curable-polydimethylsiloxane-microfluidic-chips
#16
Junbeom Kim, Heseong An, Yoojin Seo, Youngmee Jung, Jong Suk Lee, Nakwon Choi, Ki Wan Bong
Flow Lithography (FL) is the technique used for the synthesis of hydrogel microparticles with various complex shapes and distinct chemical compositions by combining microfluidics with photolithography. Although polydimethylsiloxane (PDMS) has been used most widely as almost the sole material for FL, PDMS microfluidic chips have limitations: (1) undesired shrinkage due to the thermal expansion of masters used for replica molding and (2) interfacial delamination between two thermally cured PDMS layers. Here, we propose the utilization of ultraviolet (UV)-curable PDMS (X-34-4184) for FL as an excellent alternative material of the conventional PDMS...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28469762/x-ray-transparent-microfluidic-chips-for-high-throughput-screening-and-optimization-of-in-meso-membrane-protein-crystallization
#17
Jeremy M Schieferstein, Ashtamurthy S Pawate, Chang Sun, Frank Wan, Paige N Sheraden, Jana Broecker, Oliver P Ernst, Robert B Gennis, Paul J A Kenis
Elucidating and clarifying the function of membrane proteins ultimately requires atomic resolution structures as determined most commonly by X-ray crystallography. Many high impact membrane protein structures have resulted from advanced techniques such as in meso crystallization that present technical difficulties for the set-up and scale-out of high-throughput crystallization experiments. In prior work, we designed a novel, low-throughput X-ray transparent microfluidic device that automated the mixing of protein and lipid by diffusion for in meso crystallization trials...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28405259/a549-and-mrc-5-cell-aggregation-in-a-microfluidic-lab-on-a-chip-system
#18
A Zuchowska, E Jastrzebska, K Zukowski, M Chudy, A Dybko, Z Brzozka
In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28405258/rapid-isolation-of-blood-plasma-using-a-cascaded-inertial-microfluidic-device
#19
M Robinson, H Marks, T Hinsdale, K Maitland, G Coté
Blood, saliva, mucus, sweat, sputum, and other biological fluids are often hindered in their ability to be used in point-of-care (POC) diagnostics because their assays require some form of off-site sample pre-preparation to effectively separate biomarkers from larger components such as cells. The rapid isolation, identification, and quantification of proteins and other small molecules circulating in the blood plasma from larger interfering molecules are therefore particularly important factors for optical blood diagnostic tests, in particular, when using optical approaches that incur spectroscopic interference from hemoglobin-rich red blood cells (RBCs)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396712/interplay-of-chemical-and-thermal-gradient-on-bacterial-migration-in-a-diffusive-microfluidic-device
#20
Nithya Murugesan, Purbarun Dhar, Tapobrata Panda, Sarit K Das
Living systems are constantly under different combinations of competing gradients of chemical, thermal, pH, and mechanical stresses allied. The present work is about competing chemical and thermal gradients imposed on E. coli in a diffusive stagnant microfluidic environment. The bacterial cells were exposed to opposing and aligned gradients of an attractant (1 mM sorbitol) or a repellant (1 mM NiSO4) and temperature. The effects of the repellant/attractant and temperature on migration behavior, migration rate, and initiation time for migration have been reported...
March 2017: Biomicrofluidics
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