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Biomicrofluidics

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https://www.readbyqxmd.com/read/28405259/a549-and-mrc-5-cell-aggregation-in-a-microfluidic-lab-on-a-chip-system
#1
A Zuchowska, E Jastrzebska, K Zukowski, M Chudy, A Dybko, Z Brzozka
In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28405258/rapid-isolation-of-blood-plasma-using-a-cascaded-inertial-microfluidic-device
#2
M Robinson, H Marks, T Hinsdale, K Maitland, G Coté
Blood, saliva, mucus, sweat, sputum, and other biological fluids are often hindered in their ability to be used in point-of-care (POC) diagnostics because their assays require some form of off-site sample pre-preparation to effectively separate biomarkers from larger components such as cells. The rapid isolation, identification, and quantification of proteins and other small molecules circulating in the blood plasma from larger interfering molecules are therefore particularly important factors for optical blood diagnostic tests, in particular, when using optical approaches that incur spectroscopic interference from hemoglobin-rich red blood cells (RBCs)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396712/interplay-of-chemical-and-thermal-gradient-on-bacterial-migration-in-a-diffusive-microfluidic-device
#3
Nithya Murugesan, Purbarun Dhar, Tapobrata Panda, Sarit K Das
Living systems are constantly under different combinations of competing gradients of chemical, thermal, pH, and mechanical stresses allied. The present work is about competing chemical and thermal gradients imposed on E. coli in a diffusive stagnant microfluidic environment. The bacterial cells were exposed to opposing and aligned gradients of an attractant (1 mM sorbitol) or a repellant (1 mM NiSO4) and temperature. The effects of the repellant/attractant and temperature on migration behavior, migration rate, and initiation time for migration have been reported...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396711/engineering-plant-membranes-using-droplet-interface-bilayers
#4
N E Barlow, E Smpokou, M S Friddin, R Macey, I R Gould, C Turnbull, A J Flemming, N J Brooks, O Ces, L M C Barter
Droplet interface bilayers (DIBs) have become widely recognised as a robust platform for constructing model membranes and are emerging as a key technology for the bottom-up assembly of synthetic cell-like and tissue-like structures. DIBs are formed when lipid-monolayer coated water droplets are brought together inside a well of oil, which is excluded from the interface as the DIB forms. The unique features of the system, compared to traditional approaches (e.g., supported lipid bilayers, black lipid membranes, and liposomes), is the ability to engineer multi-layered bilayer networks by connecting multiple droplets together in 3D, and the capability to impart bilayer asymmetry freely within these droplet architectures by supplying droplets with different lipids...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396710/elucidating-the-dep-phenomena-using-a-volumetric-polarization-approach-with-consideration-of-the-electric-double-layer
#5
Yu Zhao, Jozef Brcka, Jacques Faguet, Guigen Zhang
Dielectrophoretic (DEP) phenomena have been explored to great success for various applications like particle sorting and separation. To elucidate the underlying mechanism and quantify the DEP force experienced by particles, the point-dipole and Maxwell Stress Tensor (MST) methods are commonly used. However, both methods exhibit their own limitations. For example, the point-dipole method is unable to fully capture the essence of particle-particle interactions and the MST method is not suitable for particles of non-homogeneous property...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396709/human-ipsc-derived-myocardium-on-chip-with-capillary-like-flow-for-personalized-medicine
#6
Bradley W Ellis, Aylin Acun, U Isik Can, Pinar Zorlutuna
The heart wall tissue, or the myocardium, is one of the main targets in cardiovascular disease prevention and treatment. Animal models have not been sufficient in mimicking the human myocardium as evident by the very low clinical translation rates of cardiovascular drugs. Additionally, current in vitro models of the human myocardium possess several shortcomings such as lack of physiologically relevant co-culture of myocardial cells, lack of a 3D biomimetic environment, and the use of non-human cells. In this study, we address these shortcomings through the design and manufacture of a myocardium-on-chip (MOC) using 3D cell-laden hydrogel constructs and human induced pluripotent stem cell (hiPSC) derived myocardial cells...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396708/design-and-characterization-of-hydrogel-based-microfluidic-devices-with-biomimetic-solute-transport-networks
#7
Hyung-Jun Koo, Orlin D Velev
Hydrogel could serve as a matrix material of new classes of solar cells and photoreactors with embedded microfluidic networks. These devices mimic the structure and function of plant leaves, which are a natural soft matter based microfluidic system. These unusual microfluidic-hydrogel devices with fluid-penetrable medium operate on the basis of convective-diffusive mechanism, where the liquid is transported between the non-connected channels via molecular permeation through the hydrogel. We define three key designs of such hydrogel devices, having linear, T-shaped, and branched channels and report results of numerical simulation of the process of their infusion with solute carried by the incoming fluid...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396707/microfluidics-for-genome-wide-studies-involving-next-generation-sequencing
#8
REVIEW
Sai Ma, Travis W Murphy, Chang Lu
Next-generation sequencing (NGS) has revolutionized how molecular biology studies are conducted. Its decreasing cost and increasing throughput permit profiling of genomic, transcriptomic, and epigenomic features for a wide range of applications. Microfluidics has been proven to be highly complementary to NGS technology with its unique capabilities for handling small volumes of samples and providing platforms for automation, integration, and multiplexing. In this article, we review recent progress on applying microfluidics to facilitate genome-wide studies...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344727/characterization-of-vascular-permeability-using-a-biomimetic-microfluidic-blood-vessel-model
#9
Antony Thomas, Shunqiang Wang, Salman Sohrabi, Colin Orr, Ran He, Wentao Shi, Yaling Liu
The inflammatory response in endothelial cells (ECs) leads to an increase in vascular permeability through the formation of gaps. However, the dynamic nature of vascular permeability and external factors involved is still elusive. In this work, we use a biomimetic blood vessel (BBV) microfluidic model to measure in real-time the change in permeability of the EC layer under culture in physiologically relevant flow conditions. This platform studies the dynamics and characterizes vascular permeability when the EC layer is triggered with an inflammatory agent using tracer molecules of three different sizes, and the results are compared to a transwell insert study...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28090238/red-blood-cell-dynamics-in-biomimetic-microfluidic-networks-of-pulmonary-alveolar-capillaries
#10
Hagit Stauber, Dan Waisman, Netanel Korin, Josué Sznitman
The pulmonary capillary networks (PCNs) embody organ-specific microvasculatures, where blood vessels form dense meshes that maximize the surface area available for gas exchange in the lungs. With characteristic capillary lengths and diameters similar to the size of red blood cells (RBCs), seminal descriptions coined the term "sheet flow" nearly half a century ago to differentiate PCNs from the usual notion of Poiseuille flow in long straight tubes. Here, we revisit in true-scale experiments the original "sheet flow" model and devise for the first time biomimetic microfluidic platforms of organ-specific PCN structures perfused with RBC suspensions at near-physiological hematocrit levels...
January 10, 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344726/evaluation-of-disposable-microfluidic-chip-design-for-automated-and-fast-immunoassays
#11
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen, Sai Kumar
We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344725/sensitive-and-predictable-separation-of-microfluidic-droplets-by-size-using-in-line-passive-filter
#12
Ruihua Ding, W Lloyd Ung, John A Heyman, David A Weitz
Active manipulation of droplets is crucial in droplet microfluidics. However, droplet polydispersity decreases the accuracy of active manipulation. We develop a microfluidic "droplet filter" that accurately separates droplets by size. The droplet filter has a sharp size cutoff and is capable of distinguishing droplets differing in volume by 20%. A simple model explains the behavior of the droplets as they pass through the filter. We show application of the filter in improving dielectric sorting efficiency.
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28289484/microfluidic-devices-fabricated-using-fast-wafer-scale-led-lithography-patterning
#13
Pavan K Challa, Tadas Kartanas, Jérôme Charmet, Tuomas P J Knowles
Current lithography approaches underpinning the fabrication of microfluidic devices rely on UV exposure of photoresists to define microstructures in these materials. Conventionally, this objective is achieved with gas discharge mercury lamps, which are capable of producing high intensity UV radiation. However, these sources are costly, have a comparatively short lifetime, necessitate regular calibration, and require significant time to warm up prior to exposure taking place. To address these limitations we exploit advances in solid state sources in the UV range and describe a fast and robust wafer-scale laboratory exposure system relying entirely on UV-Light emitting diode (UV-LED) illumination...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28289483/dielectrophoresis-study-of-temporal-change-in-internal-conductivity-of-single-cho-cells-after-electroporation-by-pulsed-electric-fields
#14
E Salimi, K Braasch, M Butler, D J Thomson, G E Bridges
Applying sufficiently strong pulsed electric fields to a cell can permeabilize the membrane and subsequently affect its dielectric properties. In this study, we employ a microfluidic dielectrophoresis cytometry technique to simultaneously electroporate and measure the time-dependent dielectric response of single Chinese hamster ovary cells. Using experimental measurements along with numerical simulations, we present quantitative results for the changes in the cytoplasm conductivity of single cells within seconds after exposure to 100 μs duration pulsed electric fields with various intensities...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28217240/microfluidics-cell-sample-preparation-for-analysis-advances-in-efficient-cell-enrichment-and-precise-single-cell-capture
#15
REVIEW
Liang Huang, Shengtai Bian, Yinuo Cheng, Guanya Shi, Peng Liu, Xiongying Ye, Wenhui Wang
Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-high-efficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28191268/automated-electrotransformation-of-escherichia-coli-on-a-digital-microfluidic-platform-using-bioactivated-magnetic-beads
#16
J A Moore, M Nemat-Gorgani, A C Madison, M A Sandahl, S Punnamaraju, A E Eckhardt, M G Pollack, F Vigneault, G M Church, R B Fair, M A Horowitz, P B Griffin
This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompetent cells in the electrowetting-on-dieletric (EWoD) platform. On-cartridge electroporation was used to deliver oligonucleotides into the cells. In addition to the optimization of a magnetic bead-based benchtop protocol for generating and transforming electrocompetent E...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28191267/single-cell-digital-polymerase-chain-reaction-on-self-priming-compartmentalization-chip
#17
Qiangyuan Zhu, Lin Qiu, Yanan Xu, Guang Li, Ying Mu
Single cell analysis provides a new framework for understanding biology and disease, however, an absolute quantification of single cell gene expression still faces many challenges. Microfluidic digital polymerase chain reaction (PCR) provides a unique method to absolutely quantify the single cell gene expression, but only limited devices are developed to analyze a single cell with detection variation. This paper describes a self-priming compartmentalization (SPC) microfluidic digital polymerase chain reaction chip being capable of performing single molecule amplification from single cell...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28096941/transport-of-biomolecules-to-binding-partners-displayed-on-the-surface-of-microbeads-arrayed-in-traps-in-a-microfluidic-cell
#18
Xiaoxiao Chen, Thomas F Leary, Charles Maldarelli
Arrays of probe molecules integrated into a microfluidic cell are utilized as analytical tools to screen the binding interactions of the displayed probes against a target molecule. These assay platforms are useful in enzyme or antibody discovery, clinical diagnostics, and biosensing, as their ultraminiaturized design allows for high sensitivity and reduced consumption of reagents and target. We study here a platform in which the probes are first grafted to microbeads which are then arrayed in the microfluidic cell by capture in a trapping course...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28083079/inkjet-printed-silver-electrodes-on-macroporous-paper-for-a-paper-based-isoelectric-focusing-device
#19
Cristina Gaspar, Tiina Sikanen, Sami Franssila, Ville Jokinen
We demonstrate a combined printing process utilizing inkjet printing of silver electrodes and solid-ink technology for printing hydrophobic wax barriers for fabricating paper microfluidic devices with integrated electrodes. Optimized printing parameters are given for achieving conducting silver lines on the top of macroporous chromatography paper down to 250 μm-300 μm resolution. Electrical characterization and wicking experiments demonstrate that the printed silver patterns are simultaneously conductive and porous enough to allow reliable capillary wicking across the electrodes...
November 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/28058085/microfluidic-cytometers-with-integrated-on-chip-optical-systems-for-red-blood-cell-and-platelet-counting
#20
Yingying Zhao, Qin Li, Xiaoming Hu, Yuhwa Lo
A microfluidic cytometer with integrated on-chip optical systems was designed for red blood cell (RBC) and platelet (PLT) counting. The design, fabrication, and characterization of the microfluidic cytometer with on-chip optical signal detection were described. With process using only a single mask, the device that integrates optical fibers and on-chip microlens with microfluidic channels on a polydimethylsiloxane layer by standard soft photolithography. This compact structure increased the sensitivity of the device and eliminated time-consuming free-space optical alignments...
November 2016: Biomicrofluidics
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