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Eric Lepowsky, Fariba Ghaderinezhad, Stephanie Knowlton, Savas Tasoglu
A transformation of the healthcare industry is necessary and imminent: hospital-centered, reactive care will soon give way to proactive, person-centered care which focuses on individuals' well-being. However, this transition will only be made possible through scientific innovation. Next-generation technologies will be the key to developing affordable and accessible care, while also lowering the costs of healthcare. A promising solution to this challenge is low-cost continuous health monitoring; this approach allows for effective screening, analysis, and diagnosis and facilitates proactive medical intervention...
September 2017: Biomicrofluidics
Tuan Vu Quoc, Meng-Syuan Wu, Tung Thanh Bui, Trinh Chu Duc, Chun-Ping Jen
In this study, a low-cost, compact biochip is designed and fabricated for protein detection. Nanofractures formed by self-assembled gold nanoparticles at junction gaps are applied for ion enrichment and depletion to create a trapping zone when electroosmotic flow occurs in microchannels. An impedance measurement module is implemented based on the lock-in amplifier technique to measure the impedance change during antibody growth on the gold electrodes which is caused by trapped proteins in the detection region...
September 2017: Biomicrofluidics
Sitong Zhou, Yu-Shan Huang, Paul D Kingsley, Kathryn H Cyr, James Palis, Jiandi Wan
Primitive erythroblasts (precursors of red blood cells) enter vascular circulation during the embryonic period and mature while circulating. As a result, primitive erythroblasts constantly experience significant hemodynamic shear stress. Shear-induced deformation of primitive erythroblasts however, is poorly studied. In this work, we examined the deformability of primitive erythroblasts at physiologically relevant flow conditions in microfluidic channels and identified the regulatory roles of the maturation stage of primitive erythroblasts and cytoskeletal protein 4...
September 2017: Biomicrofluidics
Sijia Chen, Liguang Zhang, Yi Zhao, Ming Ke, Bo Li, Longcong Chen, Shaoxi Cai
Microfluidic technology is an important research tool for investigating angiogenesis in vitro. Here, we fabricated a polydimethylsiloxane (PDMS) microfluidic device with five cross-shaped chambers using a coverslip molding method. Then, the perforated PDMS microhole arrays prepared by soft lithography were assembled in the device as barriers; a single microhole had a diameter of 100 μm. After injecting type I collagen into the middle gel chamber, we added a culture medium containing a vascular endothelial growth factor (VEGF) into the middle chamber...
September 2017: Biomicrofluidics
Kangfu Chen, Teodor Z Georgiev, Weian Sheng, Xiangjun Zheng, Jose I Varillas, Jinling Zhang, Z Hugh Fan
Circulating tumor cells (CTCs) have shown potential for cancer diagnosis and prognosis. Affinity-based CTC isolation methods have been proved to be efficient for CTC detection in clinical blood samples. One of the popular choices for affinity-based CTC isolation is to immobilize capture agents onto an array of microposts in microchannels, providing high CTC capture efficiency due to enhanced interactions between tumor cells and capture agents on the microposts. However, how the cells interact with microposts under different flow conditions and what kind of capture pattern results from the interactions have not been fully investigated; a full understanding of these interactions will help to design devices and choose experimental conditions for higher CTC capture effeciency...
September 2017: Biomicrofluidics
Andrea L Kadilak, Jessica C Rehaag, Cameron A Harrington, Leslie M Shor
Additive manufacturing, or 3D-printing techniques have recently begun to enable simpler, faster, and cheaper production of millifluidic devices at resolutions approaching 100-200 μm. At this resolution, cell culture devices can be constructed that more accurately replicate natural environments compared with conventional culturing techniques. A number of microfluidics researchers have begun incorporating additive manufacturing into their work, using 3D-printed devices in a wide array of chemical, fluidic, and even some biological applications...
September 2017: Biomicrofluidics
Ji-Chul Hyun, Jongchan Choi, Yu-Gyung Jung, Sung Yang
In this study, a microfluidic cell concentrator with a reduced-deviation-flow herringbone structure is proposed. The reduced-deviation-flow herringbone structure reduces the magnitude of deviation flow by a factor of 3.3 compared to the original herringbone structure. This structure shows higher recovery efficiency compared to the original herringbone structure for various particle sizes at high flow rate conditions. Using the reduced-deviation-flow herringbone structure, the experimental results show a recovery efficiency of 98...
September 2017: Biomicrofluidics
Yu Chen, Robert H Austin, James C Sturm
Flow cytometry analysis requires a large amount of isolated, labelled, and purified cells for accurate results. To address the demand for a large quantity of cells prepared in a timely manner, we describe a novel microfluidic trap structure array for on-chip cell labelling, such as intracellular and extracellular labelling, and subsequent washing and release of cells. Each device contains [Formula: see text] trap structures, which made the preparation of large numbers of cells [Formula: see text] possible. The structure has a streamlined shape, which minimizes clogging of cells in capture and release steps...
September 2017: Biomicrofluidics
Jiyoung Son, Raheel Samuel, Bruce K Gale, Douglas T Carrell, James M Hotaling
Microfluidic technology has potential to separate sperm cells from unwanted debris while improving the effectiveness of assisted reproductive technologies (ART). Current clinical protocol limitations regarding the separation of sperm cells from other cells/cellular debris can lead to low sperm recovery when the sample contains a low concentration of mostly low motility sperm cells and a high concentration of unwanted cells/cellular debris, such as in semen samples from patients with pyospermia [high white blood cell (WBC) semen]...
September 2017: Biomicrofluidics
Diana Pinho, Laura Campo-Deaño, Rui Lima, Fernando T Pinho
Suspensions of healthy and pathological red blood cells (RBC) flowing in microfluidic devices are frequently used to perform in vitro blood experiments for a better understanding of human microcirculation hemodynamic phenomena. This work reports the development of particulate viscoelastic analogue fluids able to mimic the rheological and hemorheological behavior of pathological RBC suspensions flowing in microfluidic systems. The pathological RBCs were obtained by an incubation of healthy RBCs at a high concentration of glucose, representing the pathological stage of hyperglycaemia in diabetic complications, and analyses of their deformability and aggregation were carried out...
September 2017: Biomicrofluidics
Tao Geng, Chuck R Smallwood, Erin L Bredeweg, Kyle R Pomraning, Andrew E Plymale, Scott E Baker, James E Evans, Ryan T Kelly
Modern live-cell imaging approaches permit real-time visualization of biological processes, yet limitations exist for unicellular organism isolation, culturing, and long-term imaging that preclude fully understanding how cells sense and respond to environmental perturbations and the link between single-cell variability and whole-population dynamics. Here, we present a microfluidic platform that provides fine control over the local environment with the capacity to replace media components at any experimental time point, and provides both perfused and compartmentalized cultivation conditions depending on the valve configuration...
September 2017: Biomicrofluidics
Ankita Gangotra, Geoff R Willmott
We report on the use of scanning ion conductance microscopy (SICM) for in-situ topographical mapping of single tunable nanopores, which are used for tunable resistive pulse sensing. A customised SICM system was used to map the elastomeric pore membranes repeatedly, using pipettes with tip opening diameters of approximately 50 nm and 1000 nm. The effect of variations on current threshold, scanning step size, and stretching has been studied. Lowering the current threshold increased the sensitivity of the pipette while scanning, up to the point where the tip contacted the surface...
September 2017: Biomicrofluidics
M S Wiederoder, S Smith, P Madzivhandila, D Mager, K Moodley, D L DeVoe, K J Land
The presented work demonstrates novel functionalities of hybrid paper-polymer centrifugal devices for assay performance enhancement that leverage the advantages of both paper-based and centrifugal microfluidic platforms. The fluid flow is manipulated by balancing the capillary force of paper inserts with the centrifugal force generated by disc rotation to enhance the signal of a colorimetric lateral flow immunoassay for pathogenic E. coli. Low-cost centrifugation for pre-concentration of bacteria was demonstrated by sample sedimentation at high rotational speeds before supernatant removal by a paper insert via capillary force after deceleration...
September 2017: Biomicrofluidics
Orin Hemminger, Pouyan E Boukany
Despite the relevance and importance of slip, a fundamental understanding of the underlying molecular mechanisms of wall slip in polymer flow is still missing. In this work, we investigate the slip behavior of an entangled DNA solution at a molecular scale using a confocal microscope coupled to a microfluidic device. From microscopic measurement, we obtain both the velocity profile and conformation of polymeric chains by visualizing DNA molecules during flow on various surfaces (ranging from weak to strong interactions with DNA molecules)...
July 2017: Biomicrofluidics
Klaus Bonazza, Bernhard Scheichl, Johannes Frank, Hanspeter Rottensteiner, Gerald Schrenk, Gernot Friedbacher, Peter L Turecek, Friedrich Scheiflinger, Günter Allmaier
Wall shear rates are critical for a broad variety of fluidic phenomena and are taken into account in nearly every experimental or simulation study. Generally, shear rates are not observable directly but rather derived from other parameters such as pressure and flow, often assuming somehow idealized systems. However, there is a biological system which is able to constantly measure the wall shear as a part of a regulatory circuit: The blood circulation system takes advantage of shear rate sensor (protein)molecules (multimeric forms of von Willebrand Factor, VWF), which are dissolved in the blood plasma and dramatically change their conformation under shear conditions...
July 2017: Biomicrofluidics
S Altundemir, A K Uguz, K Ulgen
Paper-based microfluidics has attracted attention for the last ten years due to its advantages such as low sample volume requirement, ease of use, portability, high sensitivity, and no necessity to well-equipped laboratory equipment and well-trained manpower. These characteristics have made paper platforms a promising alternative for a variety of applications such as clinical diagnosis and quantitative analysis of chemical and biological substances. Among the wide range of fabrication methods for microfluidic paper-based analytical devices (μPADs), the wax printing method is suitable for high throughput production and requires only a commercial printer and a heating source to fabricate complex two or three-dimensional structures for multipurpose systems...
July 2017: Biomicrofluidics
Nihal G Maremanda, Kislay Roy, Rupinder K Kanwar, Vidyarani Shyamsundar, Vijayalakshmi Ramshankar, Arvind Krishnamurthy, Subramanian Krishnakumar, Jagat R Kanwar
[This corrects the article DOI: 10.1063/1.4930983.].
July 2017: Biomicrofluidics
Richard Syms
Airflow-enhanced evaporation is investigated as a method for rapid chemical preconcentration on a thin porous substrate. The mechanism is described by combining 1D models of capillary rise, chromatography, and pervaporation concentration. It is shown that the effective length of the column can be shorter than its actual length, allowing concentrate to be held at a stagnation point and then released for separation, and that the Péclet number, which determines the concentration performance, is determined only by the substrate properties...
July 2017: Biomicrofluidics
Logeeshan Velmanickam, Michael Fondakowski, Ivan T Lima, Dharmakeerthi Nawarathna
We present an integrated dielectrophoretic (DEP) and surface plasmonic technique to quantify ∼1 pM of fluorescent molecules in low conductivity buffers. We have established a DEP force on target molecules to bring those molecules and place them on the nanometallic structures (hotspots) for quantification through surface plasmonic effects. Our results show that the DEP is capable of placing the fluorescent molecules on the hotspots, which are depicted as a significant reduction in the fluorescence lifetime of those molecules...
July 2017: Biomicrofluidics
Wu Shang, Chen-Yu Tsao, Xiaolong Luo, Mairan Teodoro, Ryan McKay, David N Quan, Hsuan-Chen Wu, Gregory F Payne, William E Bentley
We have developed a user-friendly microfluidic device for the study of gradient-mediated bacterial behaviors, including chemotaxis. This device rapidly establishes linear concentration gradients by exploiting solute diffusion through porous membranes in the absence of convective flows. As such, the gradients are created rapidly and can be sustained for long time periods (e.g., hours), sufficient to evaluate cell phenotype. The device exploits a unique simple bilayer configuration that enables rapid setup and quick reproducible introduction of cells...
July 2017: Biomicrofluidics
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