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Chenyu Wang, Wenwen Liu, Qingquan Wei, Lufeng Ren, Manqing Tan, Yude Yu
Conventional cell-sized well arrays have advantages of high occupancy, simple operation, and low cost for capturing single-cells. However, they have insufficient space for including reagents required for cell treatment or analysis, which restricts the wide application of cell-sized well arrays as a single-cell research tool alone. Here, we present a novel dual-well array chip, which integrates capture-wells (20  μ m in diameter) with reaction-wells (100  μ m in diameter) and describe a flow method for convenient single-cell analysis requiring neither complicated infra-structure nor high expenditure, while enabling highly efficient single cell trapping (75...
May 2018: Biomicrofluidics
Matthew Rogers, Tammy Sobolik, David K Schaffer, Philip C Samson, Andrew C Johnson, Philip Owens, Simona G Codreanu, Stacy D Sherrod, John A McLean, John P Wikswo, Ann Richmond
The interaction of cancer cells with the stromal cells and matrix in the tumor microenvironment plays a key role in progression to metastasis. A better understanding of the mechanisms underlying these interactions would aid in developing new therapeutic approaches to inhibit this progression. Here, we describe the fabrication of a simple microfluidic bioreactor capable of recapitulating the three-dimensional breast tumor microenvironment. Cancer cell spheroids, fibroblasts, and endothelial cells co-cultured in this device create a robust microenvironment suitable for studying in real time the migration of cancer cells along matrix structures laid down by fibroblasts within the 3D tumor microenvironment...
May 2018: Biomicrofluidics
Daewon Lee, Amos Chungwon Lee, Sangkwon Han, Hyung Jong Bae, Seo Woo Song, Yunjin Jeong, Dong Yoon Oh, Seongkyu Cho, Junhoi Kim, Wook Park, Sunghoon Kwon
Understanding tissue engineering using a bottom-up approach has been hindered by technical limitations because no platform can demonstrate the controlled formation of a heterogeneous population of cells in microscale. Here, we demonstrate hierarchical shape-by-shape assembly of virus-laden particles into larger ones to transfect two different genes on the seeded cells. We show that smaller daughter particles with different sizes and shapes can be assembled into the matching indentations of larger parent particles with different sizes and shapes...
May 2018: Biomicrofluidics
Han Zhang, Lifu Xiao, Qifei Li, Xiaojun Qi, Anhong Zhou
Raman spectroscopy has been playing an increasingly significant role for cell classification. Here, we introduce a novel microfluidic chip for non-invasive Raman cell natural fingerprint collection. Traditional Raman spectroscopy measurement of the cells grown in a Polydimethylsiloxane (PDMS) based microfluidic device suffers from the background noise from the substrate materials of PDMS when intended to apply as an in vitro cell assay. To overcome this disadvantage, the current device is designed with a middle layer of PDMS layer sandwiched by two MgF2 slides which minimize the PDMS background signal in Raman measurement...
March 2018: Biomicrofluidics
Dongce Ma, Ran Wang, Shuxun Chen, Tao Luo, Yu-Ting Chow, Dong Sun
Cancer cell migration and invasion, which are involved in tumour metastasis, are hard to predict and control. Numerous studies have demonstrated that physical cues influence cancer cell migration and affect tumour metastasis. In this study, we proposed the use of a microchannel chip equipped with a number of vertical constrictions to produce periodic compression forces on cells passing through narrow channels. The chip with repeated vertical confinement was applied on adherent MHCC-97L liver cancer cells and suspended OCI-AML leukaemia cells to determine the migration ability of these cancer cells...
March 2018: Biomicrofluidics
Qin Zhang, Jibin Fan, Han Wang, Hisayuki Aoyama
[This corrects the article DOI: 10.1063/1.5020600.].
March 2018: Biomicrofluidics
Hajar Moghadas, Mohammad Said Saidi, Navid Kashaninejad, Nam-Trung Nguyen
Thin porous membranes are important components in a microfluidic device, serving as separators, filters, and scaffolds for cell culture. However, the fabrication and the integration of these membranes possess many challenges, which restrict their widespread applications. This paper reports a facile technique to fabricate robust membrane-embedded microfluidic devices. We integrated an electrospun membrane into a polydimethylsiloxane (PDMS) device using the simple plasma-activated bonding technique. To increase the flexibility of the membrane and to address the leakage problem, the electrospun membrane was fabricated with the highest weight ratio of PDMS to polymethylmethacrylate (i...
March 2018: Biomicrofluidics
Li-Jing Cheng
Electrokinetic transport of ions between electrolyte solutions and ion permselective solid media governs a variety of applications, such as molecular separation, biological detection, and bioelectronics. These applications rely on a unique class of materials and devices to interface the ionic and electronic systems. The devices built on ion permselective materials or micro-/nanofluidic channels are arranged to work with aqueous environments capable of either manipulating charged species through applied electric fields or transducing biological responses into electronic signals...
March 2018: Biomicrofluidics
Yang Jun Kang
To evaluate variations of blood circulating in closed loops, hemorheological properties including blood viscosity and red blood cells (RBCs) are quantitatively measured with independent in-vitro instruments after collecting blood from a closed loop. But, most previous methods require periodic blood collections which induce several problems such as geometric differences between the fluidic channel and the in-vitro method, hemodilution, storage time, and unspecific blood flow rates. To resolve these issues, in this study, blood viscosity and RBC aggregation of blood circulating within a closed loop are measured with a microfluidic platform periodically and simultaneously...
March 2018: Biomicrofluidics
Fan Liu, Liwei Ni, Jiang Zhe
Signal multiplexing is vital to develop lab-on-a-chip devices that can detect and quantify multiple cellular and molecular biomarkers with high throughput, short analysis time, and low cost. Electrical detection of biomarkers has been widely used in lab-on-a-chip devices because it requires less external equipment and simple signal processing and provides higher scalability. Various electrical multiplexing for lab-on-a-chip devices have been developed for comprehensive, high throughput, and rapid analysis of biomarkers...
March 2018: Biomicrofluidics
Miguel Tovar, Thomas Weber, Sundar Hengoju, Andrea Lovera, Anne-Sophie Munser, Oksana Shvydkiv, Martin Roth
In order to leverage the immense potential of droplet microfluidics, it is necessary to simplify the process of chip design and fabrication. While polydimethylsiloxane (PDMS) replica molding has greatly revolutionized the chip-production process, its dependence on 2D-limited photolithography has restricted the design possibilities, as well as further dissemination of microfluidics to non-specialized labs. To break free from these restrictions while keeping fabrication straighforward, we introduce an approach to produce complex multi-height (3D) droplet microfluidic glass molds and subsequent chip production by PDMS replica molding...
March 2018: Biomicrofluidics
Bastien Venzac, Randa Madoun, Taous Benarab, Sylvain Monnier, Fanny Cayrac, Sarah Myram, Ludovic Leconte, François Amblard, Jean-Louis Viovy, Stéphanie Descroix, Sylvie Coscoy
Multicellular tubes are structures ubiquitously found during development and in adult organisms. Their topologies (diameter, direction or branching), together with their mechanical characteristics, play fundamental roles in organ function and in the emergence of pathologies. In tubes of micrometric range diameters, typically found in the vascular system, renal tubules or excretory ducts, cells are submitted to a strong curvature and confinement effects in addition to flow. Then, small tubes with change in diameter are submitted to a local gradient of shear stress and curvature, which may lead to complex mechanotransduction responses along tubes, and may be involved in the onset or propagation of cystic or obstructive pathologies...
March 2018: Biomicrofluidics
Shweta Bhagwat, Shraddha Sontakke, Deekshith K, Priyanka Parte, Sameer Jadhav
Chemotaxis, as a mechanism for sperm guidance in vivo , is an enigma which has been difficult to demonstrate. To address this issue, various devices have been designed to study sperm chemotaxis in vitro . Limitations of traditional chemotaxis devices were related to the inability to maintain a stable concentration gradient as well as track single sperm over long times. Microfluidics technology, which provides superior control over fluid flow, has been recently used to generate stable concentration gradients for investigating the chemotactic behavior of several cell types including spermatozoa...
March 2018: Biomicrofluidics
Junsheng Wang, Xiaomei Yu, Yanjuan Wang, Xinxiang Pan, Dongqing Li
A rapid detection of micro-algae activity is critical for analysis of ship ballast water. A new method for detecting micro-algae activity based on lens-free optofluidic holographic imaging is presented in this paper. A compact lens-free optofluidic holographic imaging device was developed. This device is mainly composed of a light source, a small through-hole, a light propagation module, a microfluidic chip, and an image acquisition and processing module. The excited light from the light source passes through a small hole to reach the surface of the micro-algae cells in the microfluidic chip, and a holographic image is formed by the diffraction light of surface of micro-algae cells...
March 2018: Biomicrofluidics
Qin Zhang, Jinbin Fan, Han Wang, Hisayuki Aoyama
A particle manipulation method was presented in this paper based on opposed jets. In such a method, particles were trapped near the stagnation point of the flow field and moved by controlling the position of the stagnation point. The hold direction of the flow to the particle was changed by changing the orientation of the opposed-jet flow field where a particle is trapped. Subsequently, the directional and quantitative movement of the particle in any direction was achieved. Taking micron particles as examples, we analyzed the control mechanism of particles based on opposed jets and evaluated the influence of jet velocity, inner diameter, distance of end face, radial error, and position of capillaries on the particle control performance by simulations...
March 2018: Biomicrofluidics
Wenjia Liu, Antony Warden, Jiahui Sun, Guangxia Shen, Xianting Ding
Portable Polymerase Chain Reaction (PCR) devices combined with microfluidic chips or lateral flow stripes have shown great potential in the field of point-of-need testing (PoNT) as they only require a small volume of patient sample and are capable of presenting results in a short time. However, the detection for multiple targets in this field leaves much to be desired. Herein, we introduce a novel PCR platform by integrating a bottom-well microfluidic chip with an infra-red (IR) excited temperature control method and fluorescence co-detection of three PCR products...
March 2018: Biomicrofluidics
Xiaosong Su, Shiyin Zhang, Shengxiang Ge, Mengyuan Chen, Jianzhong Zhang, Jun Zhang, Ningshao Xia
To fulfill the requirement of sample preparation in a microfluidic analysis system designed for "sample in, answer out" testing which was urgently needed by resource limited clinical facilities, we proposed a critical low cost, membrane-based serum separator design in this article. With a specially designed microchip, this device can easily separate serum from the whole blood sample in 5 min. Different from techniques which have been reported earlier, this approach does not require either centrifugation or sample dilution which may cause hemolysis or decreased testing sensitivity...
March 2018: Biomicrofluidics
Yuanyuan Chen, Yuzhen Feng, Jiandi Wan, Haosheng Chen
Prolonged storage will alter the biophysical properties of red blood cells (RBCs), and it decreases the quality of stored blood for blood transfusion. It has been known that less deformable aged RBCs can be separated by margination, but the recognition of the storage time from the separation efficiency of the stiff RBCs is still a challenge. In this study, we realized enhanced separation of aged RBCs from normal RBCs by controlling the channel cross section and demonstrated that the storage time can be deduced from the percentage of the separated RBCs in the stored RBCs...
March 2018: Biomicrofluidics
E Werner, A Jain, A Muralidhar, K Frykholm, T St Clere Smithe, J Fritzsche, F Westerlund, K D Dorfman, B Mehlig
If a semiflexible polymer confined to a narrow channel bends around by 180°, the polymer is said to exhibit a hairpin. The equilibrium extension statistics of the confined polymer are well understood when hairpins are vanishingly rare or when they are plentiful. Here, we analyze the extension statistics in the intermediate situation via experiments with DNA coated by the protein RecA, which enhances the stiffness of the DNA molecule by approximately one order of magnitude. We find that the extension distribution is highly non-Gaussian, in good agreement with Monte-Carlo simulations of confined discrete wormlike chains...
March 2018: Biomicrofluidics
Richard O'Rorke, David Collins, Ye Ai
Acoustofluidics has a strong pedigree in microscale manipulation, with particle and cell separation and patterning arising from acoustic pressure gradients. Acoustic waveguides are a promising candidate for localizing force fields in microfluidic devices, for which computational modelling is an important design tool. Meshed finite element analysis is a popular approach for this, yet its computation time increases rapidly when complex geometries are used, limiting its usefulness. Here, we present an analytical model of the acoustic pressure field in a microchannel arising from a surface acoustic wave (SAW) boundary condition that computes in milliseconds and provide the simulation code in the supplementary material...
March 2018: Biomicrofluidics
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