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Biomicrofluidics

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https://www.readbyqxmd.com/read/28529674/erratum-publisher-s-note-maximizing-particle-concentration-in-deterministic-lateral-displacement-arrays-biomicrofluidics-11-024121-2017
#1
Shilun Feng, Alison M Skelley, Ayad G Anwer, Guozhen Liu, David W Inglis
[This corrects the article DOI: 10.1063/1.4981014.].
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529673/on-chip-two-photon-metabolic-imaging-for-drug-toxicity-testing
#2
Fang Yu, Shuangmu Zhuo, Yinghua Qu, Deepak Choudhury, Zhiping Wang, Ciprian Iliescu, Hanry Yu
We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529672/on-chip-density-based-purification-of-liposomes
#3
Siddharth Deshpande, Anthony Birnie, Cees Dekker
Due to their cell membrane-mimicking properties, liposomes have served as a versatile research tool in science, from membrane biophysics and drug delivery systems to bottom-up synthetic cells. We recently reported a novel microfluidic method, Octanol-assisted Liposome Assembly (OLA), to form cell-sized, monodisperse, unilamellar liposomes with excellent encapsulation efficiency. Although OLA provides crucial advantages over alternative methods, it suffers from the presence of 1-octanol droplets, an inevitable by-product of the production process...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529671/the-advection-of-microparticles-mcf-7-and-mda-mb-231-breast-cancer-cells-in-response-to-very-low-reynolds-numbers
#4
Sinéad T Morley, Michael T Walsh, David T Newport
The lymphatic system is an extensive vascular network that serves as the primary route for the metastatic spread of breast cancer cells (BCCs). The dynamics by which BCCs travel in the lymphatics to distant sites, and eventually establish metastatic tumors, remain poorly understood. Particle tracking techniques were employed to analyze the behavior of MCF-7 and MDA-MB-231 BCCs which were exposed to lymphatic flow conditions in a 100 μm square microchannel. The behavior of the BCCs was compared to rigid particles of various diameters (η = dp/H= 0...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529670/perspective-fabrication-of-integrated-organ-on-a-chip-via-bioprinting
#5
Qingzhen Yang, Qin Lian, Feng Xu
Organ-on-a-chip has emerged as a powerful platform with widespread applications in biomedical engineering, such as pathology studies and drug screening. However, the fabrication of organ-on-a-chip is still a challenging task due to its complexity. For an integrated organ-on-a-chip, it may contain four key elements, i.e., a microfluidic chip, live cells/microtissues that are cultured in this chip, components for stimulus loading to mature the microtissues, and sensors for results readout. Recently, bioprinting has been used for fabricating organ-on-a-chip as it enables the printing of multiple materials, including biocompatible materials and even live cells in a programmable manner with a high spatial resolution...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503248/stable-microfluidic-flow-focusing-using-hydrostatics
#6
Vaskar Gnyawali, Mohammadali Saremi, Michael C Kolios, Scott S H Tsai
We present a simple technique to generate stable hydrodynamically focused flows by driving the flow with hydrostatic pressure from liquid columns connected to the inlets of a microfluidic device. Importantly, we compare the focused flows generated by hydrostatic pressure and classical syringe pump driven flows and find that the stability of the hydrostatic pressure driven technique is significantly better than the stability achieved via syringe pumps, providing fluctuation-free focused flows that are suitable for sensitive microfluidic flow cytometry applications...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503247/reconfigurable-microfluidic-device-with-discretized-sidewall
#7
Masahiro Oono, Keisuke Yamaguchi, Amirul Rasyid, Atsushi Takano, Masato Tanaka, Nobuyuki Futai
Various microfluidic features, such as traps, have been used to manipulate flows, cells, and other particles within microfluidic systems. However, these features often become undesirable in subsequent steps requiring different fluidic configurations. To meet the changing needs of various microfluidic configurations, we developed a reconfigurable microfluidic channel with movable sidewalls using mechanically discretized sidewalls of laterally aligned rectangular pins. The user can deform the channel sidewall at any time after fabrication by sliding the pins...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503246/particle-concentrating-and-sorting-under-a-rotating-electric-field-by-direct-optical-liquid-heating-in-a-microfluidics-chip
#8
Yu-Liang Chen, Hong-Ren Jiang
We demonstrate a functional rotating electrothermal technique for rapidly concentrating and sorting a large number of particles on a microchip by the combination of particle dielectrophoresis (DEP) and inward rotating electrothermal (RET) flows. Different kinds of particles can be attracted (positive DEP) to or repelled (negative DEP) from electrode edges, and then the n-DEP responsive particles are further concentrated in the heated region by RET flows. The RET flows arise from the spatial inhomogeneous electric properties of fluid caused by direct infrared laser (1470 nm) heating of solution in a rotating electric field...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28503245/maximizing-particle-concentration-in-deterministic-lateral-displacement-arrays
#9
Shilun L Feng, Alison M Skelley, Ayad G Anwer, Guozhen Liu, David W Inglis
We present an improvement to deterministic lateral displacement arrays, which allows higher particle concentration enhancement. We correct and extend previous equations to a mirror-symmetric boundary. This approach allows particles to be concentrated into a central channel, no wider than the surrounding gaps, thereby maximizing the particle enrichment. The resulting flow patterns were, for the first time, experimentally measured. The performance of the device with hard micro-spheres and cells was investigated...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28469763/flow-lithography-in-ultraviolet-curable-polydimethylsiloxane-microfluidic-chips
#10
Junbeom Kim, Heseong An, Yoojin Seo, Youngmee Jung, Jong Suk Lee, Nakwon Choi, Ki Wan Bong
Flow Lithography (FL) is the technique used for the synthesis of hydrogel microparticles with various complex shapes and distinct chemical compositions by combining microfluidics with photolithography. Although polydimethylsiloxane (PDMS) has been used most widely as almost the sole material for FL, PDMS microfluidic chips have limitations: (1) undesired shrinkage due to the thermal expansion of masters used for replica molding and (2) interfacial delamination between two thermally cured PDMS layers. Here, we propose the utilization of ultraviolet (UV)-curable PDMS (X-34-4184) for FL as an excellent alternative material of the conventional PDMS...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28469762/x-ray-transparent-microfluidic-chips-for-high-throughput-screening-and-optimization-of-in-meso-membrane-protein-crystallization
#11
Jeremy M Schieferstein, Ashtamurthy S Pawate, Chang Sun, Frank Wan, Paige N Sheraden, Jana Broecker, Oliver P Ernst, Robert B Gennis, Paul J A Kenis
Elucidating and clarifying the function of membrane proteins ultimately requires atomic resolution structures as determined most commonly by X-ray crystallography. Many high impact membrane protein structures have resulted from advanced techniques such as in meso crystallization that present technical difficulties for the set-up and scale-out of high-throughput crystallization experiments. In prior work, we designed a novel, low-throughput X-ray transparent microfluidic device that automated the mixing of protein and lipid by diffusion for in meso crystallization trials...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28405259/a549-and-mrc-5-cell-aggregation-in-a-microfluidic-lab-on-a-chip-system
#12
A Zuchowska, E Jastrzebska, K Zukowski, M Chudy, A Dybko, Z Brzozka
In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28405258/rapid-isolation-of-blood-plasma-using-a-cascaded-inertial-microfluidic-device
#13
M Robinson, H Marks, T Hinsdale, K Maitland, G Coté
Blood, saliva, mucus, sweat, sputum, and other biological fluids are often hindered in their ability to be used in point-of-care (POC) diagnostics because their assays require some form of off-site sample pre-preparation to effectively separate biomarkers from larger components such as cells. The rapid isolation, identification, and quantification of proteins and other small molecules circulating in the blood plasma from larger interfering molecules are therefore particularly important factors for optical blood diagnostic tests, in particular, when using optical approaches that incur spectroscopic interference from hemoglobin-rich red blood cells (RBCs)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396712/interplay-of-chemical-and-thermal-gradient-on-bacterial-migration-in-a-diffusive-microfluidic-device
#14
Nithya Murugesan, Purbarun Dhar, Tapobrata Panda, Sarit K Das
Living systems are constantly under different combinations of competing gradients of chemical, thermal, pH, and mechanical stresses allied. The present work is about competing chemical and thermal gradients imposed on E. coli in a diffusive stagnant microfluidic environment. The bacterial cells were exposed to opposing and aligned gradients of an attractant (1 mM sorbitol) or a repellant (1 mM NiSO4) and temperature. The effects of the repellant/attractant and temperature on migration behavior, migration rate, and initiation time for migration have been reported...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396711/engineering-plant-membranes-using-droplet-interface-bilayers
#15
N E Barlow, E Smpokou, M S Friddin, R Macey, I R Gould, C Turnbull, A J Flemming, N J Brooks, O Ces, L M C Barter
Droplet interface bilayers (DIBs) have become widely recognised as a robust platform for constructing model membranes and are emerging as a key technology for the bottom-up assembly of synthetic cell-like and tissue-like structures. DIBs are formed when lipid-monolayer coated water droplets are brought together inside a well of oil, which is excluded from the interface as the DIB forms. The unique features of the system, compared to traditional approaches (e.g., supported lipid bilayers, black lipid membranes, and liposomes), is the ability to engineer multi-layered bilayer networks by connecting multiple droplets together in 3D, and the capability to impart bilayer asymmetry freely within these droplet architectures by supplying droplets with different lipids...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396710/elucidating-the-dep-phenomena-using-a-volumetric-polarization-approach-with-consideration-of-the-electric-double-layer
#16
Yu Zhao, Jozef Brcka, Jacques Faguet, Guigen Zhang
Dielectrophoretic (DEP) phenomena have been explored to great success for various applications like particle sorting and separation. To elucidate the underlying mechanism and quantify the DEP force experienced by particles, the point-dipole and Maxwell Stress Tensor (MST) methods are commonly used. However, both methods exhibit their own limitations. For example, the point-dipole method is unable to fully capture the essence of particle-particle interactions and the MST method is not suitable for particles of non-homogeneous property...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396709/human-ipsc-derived-myocardium-on-chip-with-capillary-like-flow-for-personalized-medicine
#17
Bradley W Ellis, Aylin Acun, U Isik Can, Pinar Zorlutuna
The heart wall tissue, or the myocardium, is one of the main targets in cardiovascular disease prevention and treatment. Animal models have not been sufficient in mimicking the human myocardium as evident by the very low clinical translation rates of cardiovascular drugs. Additionally, current in vitro models of the human myocardium possess several shortcomings such as lack of physiologically relevant co-culture of myocardial cells, lack of a 3D biomimetic environment, and the use of non-human cells. In this study, we address these shortcomings through the design and manufacture of a myocardium-on-chip (MOC) using 3D cell-laden hydrogel constructs and human induced pluripotent stem cell (hiPSC) derived myocardial cells...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396708/design-and-characterization-of-hydrogel-based-microfluidic-devices-with-biomimetic-solute-transport-networks
#18
Hyung-Jun Koo, Orlin D Velev
Hydrogel could serve as a matrix material of new classes of solar cells and photoreactors with embedded microfluidic networks. These devices mimic the structure and function of plant leaves, which are a natural soft matter based microfluidic system. These unusual microfluidic-hydrogel devices with fluid-penetrable medium operate on the basis of convective-diffusive mechanism, where the liquid is transported between the non-connected channels via molecular permeation through the hydrogel. We define three key designs of such hydrogel devices, having linear, T-shaped, and branched channels and report results of numerical simulation of the process of their infusion with solute carried by the incoming fluid...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28396707/microfluidics-for-genome-wide-studies-involving-next-generation-sequencing
#19
REVIEW
Sai Ma, Travis W Murphy, Chang Lu
Next-generation sequencing (NGS) has revolutionized how molecular biology studies are conducted. Its decreasing cost and increasing throughput permit profiling of genomic, transcriptomic, and epigenomic features for a wide range of applications. Microfluidics has been proven to be highly complementary to NGS technology with its unique capabilities for handling small volumes of samples and providing platforms for automation, integration, and multiplexing. In this article, we review recent progress on applying microfluidics to facilitate genome-wide studies...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344727/characterization-of-vascular-permeability-using-a-biomimetic-microfluidic-blood-vessel-model
#20
Antony Thomas, Shunqiang Wang, Salman Sohrabi, Colin Orr, Ran He, Wentao Shi, Yaling Liu
The inflammatory response in endothelial cells (ECs) leads to an increase in vascular permeability through the formation of gaps. However, the dynamic nature of vascular permeability and external factors involved is still elusive. In this work, we use a biomimetic blood vessel (BBV) microfluidic model to measure in real-time the change in permeability of the EC layer under culture in physiologically relevant flow conditions. This platform studies the dynamics and characterizes vascular permeability when the EC layer is triggered with an inflammatory agent using tracer molecules of three different sizes, and the results are compared to a transwell insert study...
March 2017: Biomicrofluidics
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