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Reproductive Sciences

Mariko Miyashita, Kaori Koga, Arisa Takeuchi, Tomoko Makabe, Ayumi Taguchi, Yoko Urata, Gentaro Izumi, Masashi Takamura, Miyuki Harada, Tetsuya Hirata, Yasushi Hirota, Osamu Wada-Hiraike, Osamu Yoshino, Tomoyuki Fujii, Yutaka Osuga
OBJECTIVE: The aim of this study was to clarify the expression of Ninj1 in endometriosis and adenomyosis lesions, and its inductive factor in human endometriotic stromal cells (ESCs). BACKGROUND: Nerve injury-induced protein 1 (Ninj1) is a molecule originally identified in dorsal root ganglion neurons and Schwann cells after nerve injury and promotes neurite outgrowth. The aim of this study was to clarify the expression of Ninj1 in endometriosis and adenomyosis lesions, and its inductive factor in human endometriotic stromal cells (ESCs)...
October 16, 2018: Reproductive Sciences
Chunyan Li, Biliang Chen, Jiao Zheng, Lu Cheng, Tingting Song, Fenfen Guo, Hui Xu, Feng Yan, Ying Xu, Yu Li, Jianfang Zhang
OBJECTIVE: To evaluate the diagnostic accuracy of the BACs-on-Beads (BoBs) assay for the rapid diagnosis of common aneuploidies and microdeletion syndromes. METHODS: BACs-on-Beads and chromosomal karyotyping were used for detecting 3647 cases of amniotic fluid samples with indications for prenatal diagnosis, which were collected from January 2015 to June 2017 in Xijing Hospital. Fluorescence in situ hybridization (FISH) or chromosomal microarray analysis (CMA) provided further validation...
October 16, 2018: Reproductive Sciences
Marina Nikolaeva, Alina Babayan, Elena Stepanova, Alla Arefieva, Tatiana Dontsova, Veronika Smolnikova, Elena Kalinina, Lubov Krechetova, Stanislav Pavlovich, Gennady Sukhikh
Seminal plasma (SP) is thought to be a crucial factor which affects the expansion of regulatory T cells (Tregs) in female reproductive tract during embryo implantation. We propose that seminal transforming growth factor (TGF) β1 is responsible for local accumulation of circulating Tregs, which manifests as changes in Treg frequency in peripheral blood, whereas seminal interleukin (IL) 18 interferes with TGF-β1-dependent cellular reactions. The purpose of the present study is to determine whether the frequency of circulating Tregs is associated with the levels of seminal cytokines and pregnancy establishment in women exposed to partner's SP during in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycle...
October 16, 2018: Reproductive Sciences
Steffen Kunzmann, Barbara Ottensmeier, Christian P Speer, Markus Fehrholz
Pregnant women at risk of preterm labor routinely receive glucocorticoids (GCs) and frequently also progesterone. Administration of GCs accelerates intrauterine surfactant synthesis and lung maturation, thereby reducing the incidence of neonatal respiratory distress syndrome; progesterone has the potential to prevent preterm birth. Little is known about possible interactions of GCs and progesterone. Our aim was to clarify whether progesterone can affect dexamethasone (DXM)-regulated expression of surfactant protein A (SP-A), SP-B, and SP-D in lung epithelial cells...
October 15, 2018: Reproductive Sciences
Ivo Brosens, Joanne Muter, Lauren Ewington, Patrick Puttemans, Felice Petraglia, Jan J Brosens, Giuseppe Benagiano
Preeclampsia is an important cause of maternal and perinatal morbidity, especially in first-time pregnant adolescent women. Although prevention of preeclampsia has been attempted for many decades, effective intervention can only be achieved upon the full elucidation of the risk factors and mechanisms of disease. As the pathogenesis of preeclampsia during adolescence may differ from that in older women, preventive interventions should be tailored accordingly. During adolescence, 4 putative drivers of preeclampsia can be identified...
October 15, 2018: Reproductive Sciences
Kalle T Rytkönen, Eric M Erkenbrack, Matti Poutanen, Laura L Elo, Mihaela Pavlicev, Günter P Wagner
Decidual stromal cells differentiate from endometrial stromal fibroblasts (ESFs) under the influence of progesterone and cyclic adenosine monophosphate (cAMP) and are essential for implantation and the maintenance of pregnancy. They evolved in the stem lineage of placental (eutherian) mammals coincidental with the evolution of implantation. Here we use the well-established in vitro decidualization protocol to compare early (3 days) and late (8 days) gene transcription patterns in immortalized human ESF. We document extensive, dynamic changes in the early and late decidual cell transcriptomes...
October 11, 2018: Reproductive Sciences
Elisa Borghi, Valentina Massa, Marco Severgnini, Grazia Fazio, Laura Avagliano, Elena Menegola, Gaetano Pietro Bulfamante, Giulia Morace, Francesca Borgo
The widely accepted dogma of intrauterine sterility and initial colonization of the newborn during birth has been blurred by recent observations of microbial presence in meconium, placenta, and amniotic fluid. Given the importance of a maternal-derived in utero infant seeding, it is crucial to exclude potential environmental or procedural contaminations and to assess fetal colonization before parturition. To this end, we analyzed sterilely collected intestinal tissues, placenta, and amniotic fluid from rodent fetuses and tissues from autoptic human fetuses...
October 11, 2018: Reproductive Sciences
Zilin Lin, Dehao Li, Wenjia Cheng, Jiajia Wu, Kun Wang, Yi Hu
MicroRNA (miR)-181 has been reported to participate in carcinogenesis and tumor progression in several malignant cancers, but its expression and biological functions in ovarian cancer have remained largely unclarified. Here, we first measured miR-181 expression in clinical ovarian cancers and found the expression levels of miR-181 were significantly lower in ovarian cancer tissues than that in adjacent tissues. Next, we screened and identified a direct miR-181 target, Rhotekin2 (RTKN2). A correlation between miR-181 and RTKN2 expression was also confirmed in clinical samples of ovarian cancers...
October 11, 2018: Reproductive Sciences
Fanfan Li, Yin Xie, Yuanyuan Wu, Mengzhou He, Meitao Yang, Yao Fan, Xuanxuan Li, Fuyuan Qiao, Dongrui Deng
Preeclampsia (PE) remains the leading cause of maternal and fetal morbidity and mortality. Excessive apoptosis of the placenta and poor remodeling of spiral arteries caused by insufficient invasion of trophoblast cells into uterus have been implicated in the pathogenesis of PE. Accumulating evidence showed that heat shock protein 20 (HSP20) is closely associated with the proliferation, apoptosis, and metastasis of tumor cells. However, little is known about whether HSP20 plays a role in the development of PE...
October 10, 2018: Reproductive Sciences
Megan M Thomas, Maricela Haghiac, Catalin Grozav, Judi Minium, Virtu Calabuig-Navarro, Perrie O'Tierney-Ginn
Placental fatty acid oxidation (FAO) is impaired and lipid storage is increased in pregnancy states associated with chronic oxidative stress. The effect of acute oxidative stress, as seen in pregnancies complicated with asthma, on placental lipid metabolism is unknown. We hypothesized that induction of acute oxidative stress would decrease FAO and increase esterification. We assessed [3 H]-palmitate oxidation and esterification in term placental explants from lean women after exposure to hydrogen peroxide (H2 O2 ) for 4 hours...
October 10, 2018: Reproductive Sciences
Christopher Nold, Julie Stone, Kathleen O'Hara, Patricia Davis, Vladislav Kiveliyk, Vanessa Blanchard, Steven M Yellon, Anthony T Vella
OBJECTIVE: A multitude of factors promotes inflammation in the reproductive tract leading to preterm birth. Macrophages peak in the cervix prior to birth and their numbers are increased by the cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). We hypothesize GM-CSF is produced from multiple sites in the genital tract and is a key mediator in preterm birth. STUDY DESIGN: Ectocervical, endocervical, and amniotic fluid mesenchymal stem cells were treated with lipopolysaccharide (LPS), and the concentration and expression of GM-CSF was measured...
October 8, 2018: Reproductive Sciences
Luis Alejandro Arias-Sosa
In modern society, fertility problems and demand of treatment seem to be on the rise, which led to an increased interest in research regarding human reproduction. Among these efforts, the study of the molecular senescence process has gain notorious popularity as aging is one of the most important variables involved in reproductive capacity and since the comprehension of telomere dynamics has become an important and influential theme. This new knowledge regarding the reproductive aging process is expected to offer new tools to understand the acquisition, maintenance, and loss of fertility potential...
October 8, 2018: Reproductive Sciences
Andrea Busnelli, Debora Lattuada, Stefania Ferrari, Marco Reschini, Barbara Colciaghi, Edgardo Somigliana, Luigi Fedele, Enrico Ferrazzi
Inflammation and oxidative stress are intrinsically linked to early poor placentation, typical of pregnancies complicated by preeclampsia associated with intrauterine growth restriction (PE-IUGR). Low mitochondrial DNA copy number (mtDNAcn) in peripheral blood constitutes a good peripheral surrogate marker of inflammation and oxidative stress. On these basis, we explored a possible correlation between mtDNAcn in peripheral blood in the first trimester of pregnancy and the PE-IUGR onset. To shed light on this issue, we setup a nested case-control study from a prospective cohort of pregnant women undergoing first-trimester aneuploidies screening...
October 8, 2018: Reproductive Sciences
Sema Avci, Mehmet Simsek, Hakan Soylu, Ismail Ustunel
The complex and multifactorial mechanisms that initiate and sustain the early labor process in the human uterus and cervix are still not well defined. Cervical maturation or ripening is likely to play a key role in preparing for birth. Prostaglandins have many different functions, including the regulation of uterine contractility and structure during pregnancy. The prostaglandin E1 analogue misoprostol is frequently used as a uterotonic and cervical ripening agent. Notch is a transmembrane receptor family responsible for basic functions such as cell survival, cell-cell communication, and differentiation and decidualization in pregnancy...
October 3, 2018: Reproductive Sciences
Nao Kato, Akira Iwase, Chiharu Ishida, Takashi Nagai, Masahiko Mori, Bayasula, Tomoko Nakamura, Satoko Osuka, Umida Ganiyeva, Ying Qin, Rika Miki, Fumitaka Kikkawa
Several features exist that distinguish endometriotic cells from eutopic endometrial cells. Progesterone resistance is one of the main distinguishing features, although how progesterone resistance affects the phenotype of endometriotic cells is not fully elucidated. Heart and neural crest derivatives expressed 2 (HAND2) is a transcriptional factor that plays an important role in maintaining endometrial function in a progesterone-dependent manner. Therefore, we explored whether progesterone-dependent HAND2 is implicated in the progression of endometriosis...
October 1, 2018: Reproductive Sciences
Hangying Lou, Fang Le, Minhao Hu, Xinyun Yang, Lejun Li, Liya Wang, Ning Wang, Huijuan Gao, Fan Jin
Given the higher risk of developing imprinting disorders in assisted reproductive technology (ART)-conceived children, we hypothesized that ART may affect DNA methylation of the insulin-like growth factor 2 (IGF2), H19, small nuclear ribonucleoprotein polypeptide N (SNRPN) differentially methylated regions (DMRs) at the fetal stage, which in turn may be associated with sperm abnormalities. A total of 4 patient groups were recruited, namely, multifetal reduction following in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI; n = 56), multifetal reduction following controlled ovarian hyperstimulation (COH; n = 42), male patients with normal semen parameters denoted as normozoospermia group (NZ) for IVF (n = 36), and male patients presenting with asthenozoospermia (OAZ) for ICSI (n = 38)...
October 1, 2018: Reproductive Sciences
Nigar Sofiyeva, Timo Siepmann, Kristian Barlinn, Emre Seli, Baris Ata
OBJECTIVE: A systematic review and meta-analysis was conducted to investigate whether gonadotropin-releasing hormone analogs (GnRHa) have a protective role in women treated with alkylating agents. DATA SOURCES: Major databases (PubMED, EMBASE, Cochrane Central Register of Controlled Trials), systematic snowballing, and trial registries were screened from the inception dates until September 2017. Methods and Study Selection Comparative studies involving reproductive-aged women undergoing chemotherapy with or without coadministration of GnRHa were included...
October 1, 2018: Reproductive Sciences
Puyu Yang, Zhangxin Wu, Caihong Ma, Ningning Pan, Yang Wang, Liying Yan
BACKGROUND: Differentially expressed microRNAs (miRNAs) and their target mRNAs may lead to alterations in normal physiological status of the tissues and initiate pathological processes. The aim of this study was to investigate the expression of the most relevant miRNAs in the eutopic endometrial tissue during the window of implantation in women with endometriosis-related infertility. METHODS: In the study, 76 infertile women with a regular menstrual cycle were recruited from the Center for Reproductive Medicine, Peking University Third Hospital between January 2014 and June 2016...
September 19, 2018: Reproductive Sciences
Mary Lourdes Montenegro, Camila M Bonocher, Juliana Meola, Rafael L Portella, Alfredo Ribeiro-Silva, Mariangela O Brunaldi, Rui Alberto Ferriani, Julio Cesar Rosa-E-Silva
OBJECTIVE: Endometriosis is characterized by the growth of endometrial tissue outside the uterine cavity. The prevalence of endometriosis among women experiencing pain, infertility, or both is as high as 35% to 50%. The most common symptoms of endometriosis are dysmenorrhea, dyspareunia, chronic pelvic pain, and infertility. Evidence has suggested that endometriosis symptoms result from a local inflammatory peritoneal reaction caused by ectopic endometrial implants that undergo cyclic bleeding...
September 19, 2018: Reproductive Sciences
Haruhisa Konishi, Satoshi Urabe, Hiroshi Miyoshi, Yuko Teraoka, Tomoko Maki, Hisako Furusho, Mutsumi Miyauchi, Takashi Takata, Yoshiki Kudo, Shunichi Kajioka
Inflammation is associated with preterm birth. We previously described a mouse model of chronic inflammation-induced preterm birth after dental Porphyromonas gingivalis infection. The aim of this study was to employ this model system to investigate the mechanisms through which enhanced uterine contractility induces preterm birth. Messenger RNA (mRNA) encoding contraction-associated proteins, such as oxytocin receptors, was measured at various gestational time points by real-time polymerase chain reaction (PCR)...
September 17, 2018: Reproductive Sciences
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