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Reproductive Sciences

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https://www.readbyqxmd.com/read/28205459/effects-of-an-antagonistic-analog-of-growth-hormone-releasing-hormone-on-endometriosis-in-a-mouse-model-and-in-vitro
#1
Frank Köster, Li Jin, Yuanming Shen, Andrew V Schally, Ren-Zhi Cai, Norman L Block, Daniela Hornung, Gabriele Marschner, Achim Rody, Jörg B Engel, Dominique Finas
Endometriosis is a benign gynecologic disorder causing dysmenorrhea, pelvic pain, and subfertility. Receptors for the growth hormone-releasing hormone (GHRH) were found in endometriotic tissues. Antagonists of GHRH have been used to inhibit the growth of endometriotic endometrial stromal cells. In this study, the GHRH receptor splice variant (SV) 1 was detected in human endometrial tissue samples by Western blots and quantitative reverse transcription polymerase chain reaction (qRT-PCR). The highest messenger RNA (mRNA) and protein levels of SV1 were found in eutopic endometrium from patients with endometriosis compared to ectopic endometriotic tissues and endometrium from normal patients...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28183228/btg3-overexpression-suppresses-the-proliferation-and-invasion-in-epithelial-ovarian-cancer-cell-by-regulating-akt-gsk3%C3%AE-%C3%AE-catenin-signaling
#2
Qi An, Yan Zhou, Chao Han, Yubing Zhou, Feng Li, Duolu Li
Epithelial ovarian cancer (EOC) is the leading cause of cancer-related death among all the gynecological malignancies of the female genital system, and its incidence and mortality rates continue to rise. B-cell translocation gene 3 (BTG3) plays an important role in the occurrence and development of numerous cancers. However, the role of BTG3 in EOC remains poorly understood. In this study, we aimed to investigate the biological role and potential molecular mechanism of BTG3 in EOC. We found that BTG3 protein expression was significantly lower in human EOC cell lines...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28183227/molecular-characteristics-of-the-endometrium-in-uterine-adenomyosis-and-its-biochemical-microenvironment
#3
Chih-Feng Yen, S Joseph Huang, Chyi-Long Lee, Hsin-Shih Wang, Shuen-Kuei Liao
Adenomyosis, which manifests with focally or diffusely scattered endometrial tissue within the uterine myometrium, is an endometriosis-like disease with controversial pathogenesis and compromised reproductive outcomes. This review, including the in vitro and in vivo studies performed on human or mouse models, is aimed to summarize the specific molecular characteristics of endometrium in the biochemical microenvironments of uterine adenomyosis. Many studies attributed the endometrium as the main cause of pathogenesis, with evidence of differential genetic expression and/or epigenetic modulation as well as estrogen-induced epithelial-mesenchymal transition...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28173742/ultrastructural-evaluation-of-eutopic-endometrium-of-infertile-women-with-and-without-endometriosis-during-the-window-of-implantation
#4
Michele G Da Broi, Carlos V Rocha, Filomena M Carvalho, Wellington P Martins, Rui A Ferriani, Paula A Navarro
Endometriosis is frequently associated with infertility and it is believed that the impairment of endometrial receptivity may be one of the mechanisms involved in this condition. Pinopodes are endometrial cycle-dependent structures that seem to participate in embryo implantation, and alterations in their presence and/or morphology during the window of implantation could affect the endometrial receptivity and be involved in the disease-related infertility. However, the data on pinopodes in these women are scarce and inconclusive...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28136130/integrin-%C3%AE-8-is-abundant-in-human-rat-and-mouse-trophoblasts
#5
Sebastian Herdl, Hanna Huebner, Gudrun Volkert, Ines Marek, Carlos Menendez-Castro, Stephanie C Noegel, Matthias Ruebner, Wolfgang Rascher, Andrea Hartner, Fabian B Fahlbusch
OBJECTIVE: Integrins exert regulatory functions in placentogenesis. Null mutation of certain integrin α subunits leads to placental defects with subsequent fetal growth restriction or embryonic lethality in mice. So far, the placental role of α8 integrin remains to be determined. METHODS: Localization of α8 integrin and its ligands, fibronectin (FN) and osteopontin (OPN), was studied by immunohistochemistry in human, rat, and mouse placenta. The vascularization of the placental labyrinth layer of α8 integrin-deficient mice was determined by CD31 staining...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28122483/progesterone-induced-mir-152-inhibits-the-proliferation-of-endometrial-epithelial-cells-by-downregulating-wnt-1
#6
Li Nie, You-Bo Zhao, Jun-Li Pan, Yi Lei, Min Liu, Yun Long, Jin-Hu Zhang, Ying Hu, Ming-Qian Xu, Dong-Zhi Yuan, Li-Min Yue
Progesterone (P4) is an important ovarian hormone that inhibits estrogen-dependent proliferation of endometrial epithelial cells (EECs). miR-152 has been reported to be a cell cycle regulator. In this study, we first demonstrated that P4 induced the expression of miR-152 in ovariectomized mice and Ishikawa cell. miR-152 was detected in the human endometrial cell lines that were stably transfected with P4 receptor. Results showed that P4 induced its expression through its receptor B subtype. Then, using the specific miRNA mimic and inhibitor, we proved that miR-152 impeded G1/S transition in the cell cycle of EECs and inhibited cellular proliferation via downregulating WNT-1 in mice and human endometrial cancer cell lines (Ishikawa, HEC-1-b, and KLE)...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28122482/identification-of-differentially-expressed-k-ras-transcript-variants-in-patients-with-leiomyoma
#7
Nooshin Zolfaghari, Shirin Shahbazi, Mahnaz Torfeh, Maryam Khorasani, Mehrdad Hashemi, Reza Mahdian
PURPOSE: Molecular studies have demonstrated a wide range of gene expression variations in uterine leiomyoma. The rat sarcoma virus/rapidly accelerated fibrosarcoma/mitogen-activated protein kinase (RAS/RAF/MAPK) is the crucial cellular pathway in transmitting external signals into nucleus. Deregulation of this pathway contributes to excessive cell proliferation and tumorigenesis. The present study aims to investigate the expression profile of the K-Ras transcripts in tissue samples from patients with leiomyoma...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28122481/preperitoneal-fat-thicknesses-lipid-profile-and-oxidative-status-in-women-with-uterine-fibroids
#8
Arianna Vignini, Jacopo Sabbatinelli, Nicolò Clemente, Giovanni Delli Carpini, Marta Tassetti, Giulia Zagaglia, Andrea Ciavattini
PURPOSE: There is growing evidence supporting a possible role for metabolic syndrome and its determinants, such as dyslipidemia, in uterine fibroid (UF) pathogenesis. The present study aims to investigate the association between UFs and visceral and subcutaneous fat thickness (SFT), lipid profile, and oxidative and antioxidative status. METHODS: In this cross-sectional study, 35 patients diagnosed with UFs and 15 women without UFs were enrolled. Clinical history and anthropometric parameters were collected for every woman...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28122480/a-role-for-the-inflammasome-in-spontaneous-preterm-labor-with-acute-histologic-chorioamnionitis
#9
Nardhy Gomez-Lopez, Roberto Romero, Yi Xu, Olesya Plazyo, Ronald Unkel, Yaozhu Leng, Nandor Gabor Than, Tinnakorn Chaiworapongsa, Bogdan Panaitescu, Zhong Dong, Adi L Tarca, Vikki M Abrahams, Lami Yeo, Sonia S Hassan
Inflammasomes are cytosolic multiprotein complexes that orchestrate inflammation in response to pathogens and endogenous danger signals. Herein, we determined whether the chorioamniotic membranes from women in spontaneous preterm labor with acute histologic chorioamnionitis (1) express major inflammasome components; (2) express caspase (CASP)-1 and CASP-4 as well as their active forms; (3) exhibit apoptosis-associated speck-like protein containing a CARD (ASC)/CASP-1 complex formation; and (4) release the mature forms of interleukin (IL)-1β and IL-18...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28110633/h-k-atpase-inhibition-causes-left-right-aortic-arch-inversion-in-mouse-development
#10
Yukihisa Miyachi
An organ known as a "node" forms during embryogenesis and plays a vital role in determining laterality in vertebrates. However, according to some reports in vertebrates, left-right patterning may be determined long before the node has developed. In this study, we analyzed left-right asymmetry formation in mammals based on ion-signaling factors, which has never been attempted before. First, a proton pump inhibitor was injected into pregnant mice to investigate whether H(+)/K(+)-ATPase is involved in the differentiation of pharyngeal arch arteries during embryonic development...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28110632/peritoneal-fluid-from-infertile-women-with-minimal-mild-endometriosis-compromises-the-meiotic-spindle-of-metaphase-ii-bovine-oocytes
#11
Bruna Talita Gazeto Melo Jianini, Vanessa Silvestre Innocenti Giorgi, Michele Gomes Da Broi, Cláudia Cristina Paro de Paz, Júlio César Rosa E Silva, Rui Alberto Ferriani, Paula Andrea Navarro
Some studies have demonstrated alterations in the composition of peritoneal fluid (PF) from women with endometriosis. Controversial studies have suggested that impaired oocyte quality may be involved in the pathogenesis of endometriosis-related infertility. The aim of this study was to evaluate the spindle and chromosome distribution of in vitro-matured oocytes in the presence of 2 concentrations of PF from infertile women with minimal/mild endometriosis (EI/II) compared to fertile controls. We performed an experimental study using a bovine model...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28100115/paternal-age-is-not-associated-with-pregnancy-outcomes-after-single-thawed-euploid-blastocyst-transfer
#12
Ashley W Tiegs, Nidhee M Sachdev, Jamie A Grifo, David H McCulloh, Frederick Licciardi
Although controversial, increasing paternal age has been shown to negatively affect assisted reproductive technology (ART) outcomes and success rates. Most studies investigating the effect of paternal age on ART outcomes use a donor oocyte model to minimize maternal aneuploidy contribution. This study sought to determine whether increasing paternal age is associated with adverse in vitro fertilization (IVF) outcomes when aneuploidy is minimized using preimplantation genetic screening. There were 573 single thawed euploid embryo transfers from 473 patients undergoing oocyte donor and autologous IVF cycles...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28100109/stem-cell-trafficking-during-endometriosis
#13
Antonio Simone Laganà, Francesca Maria Salmeri, Salvatore Giovanni Vitale, Onofrio Triolo, Martin Götte
No abstract text is available yet for this article.
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28093054/impact-of-psychological-stress-on-pain-perception-in-an-animal-model-of-endometriosis
#14
Siomara Hernandez, Myrella L Cruz, Inevy I Seguinot, Annelyn Torres-Reveron, Caroline B Appleyard
PURPOSE: Pain in patients with endometriosis is considered a significant source of stress but does not always correlate with severity of the condition. We have demonstrated that stress can worsen endometriosis in an animal model. Here, we tested the impact of a psychological stress protocol on pain thresholds and pain receptors. METHODS: Endometriosis was induced in female rats by suturing uterine horn tissue next to the intestinal mesentery. Sham rats had sutures only...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28093048/myofibroblasts-are-evidence-of-chronic-tissue-microtrauma-at-the-endometrial-myometrial-junctional-zone-in-uteri-with-adenomyosis
#15
Mohamed Gamal Ibrahim, Martin Sillem, Johanna Plendl, Vito Chiantera, Jalid Sehouli, Sylvia Mechsner
BACKGROUND: Adenomyosis (AM) uteri exhibit hyperperistalsis. The latter causes a chronic tissue trauma at the endometrial-myometrial junctional zone (EMJZ). Upon tissue trauma, microdehiscences in the myometrium facilitate the translocation of basal endometrial fragments into the myometrium. There, a metaplasia (mediated by transforming growth factor β1 [TGFβ1] and connective tissue growth factor [CTGF]) occurs and AM lesions develop. The abundance of myofibroblasts in a tissue hallmarks metaplasia and points to a tissue microtrauma...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28093041/blastocyst-development-in-a-single-medium-compared-to-sequential-media
#16
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, George K Petsas, Basil C Tarlatzis, Trifon G Lainas
The aim of the present study was to compare blastocyst formation rates after embryo culture in a single medium (Global) as compared to sequential media (ISM1/BlastAssist). In this prospective trial with sibling oocytes, 542 metaphase II (MII) oocytes from 31 women were randomly and equally divided to be fertilized and cultured to the blastocyst stage in either sequential media (ISM1/BlastAssist; n = 271 MII oocytes) or a single medium (Global; n = 271 MII oocytes). In both groups, embryos were cultured in an interrupted fashion with media changes on day 3...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28071357/effects-of-the-kir7-1-blocker-vu590-on-spontaneous-and-agonist-induced-contractions-of-human-pregnant-myometrium
#17
Denis J Crankshaw, David A Crosby, John J Morrison
KIR7.1, an inwardly rectifying K(+) channel, plays a critical role in regulating uterine excitability during pregnancy and has been suggested as a potential new target for the treatment of conditions arising from dysfunctional uterine contractility, for example, atonic postpartum hemorrhage. The aim of this study was to investigate the effects of the selective KIR7.1 blocker, VU590, on both spontaneous and agonist-stimulated contractions of human pregnant myometrium in vitro. At a concentration of 20 µmol/L, VU590 significantly increased the mean contractile force and the frequency of spontaneous contractions ( P < 0...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28056703/does-gnrh-agonist-triggering-control-painful-symptom-scores-during-assisted-reproductive-technology-a-retrospective-study
#18
Mathilde Bourdon, Pietro Santulli, Dominique de Ziegler, Vanessa Gayet, Chloé Maignien, Louis Marcellin, Charles Chapron
OBJECTIVE: The aim of this study was to assess the progression of pain symptoms during assisted reproductive technology (ART) cycles following administration of GnRH agonist (GnRHa) versus human chorionic gonadotrophin (hCG) triggering. DESIGN: Observational cohort study. SETTING: A tertiary care university hospital in France. POPULATION: Patients who underwent ART programs. METHODS: Between January 01, 2014, and June 31, 2014, 122 cycles were allocated to 2 groups: GnRHa triggering with a scheduled differed embryo transfer (n = 57) or hCG triggering with a fresh embryo transfer (n = 70)...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/27932593/dexamethasone-induced-intrauterine-growth-restriction-is-associated-with-altered-expressions-of-metastasis-tumor-antigens-and-cell-cycle-control-proteins-in-rat-placentas
#19
Mariam Alqaryyan, Narayana Kilarkaje, Abdeslam Mouihate, Maie D Al-Bader
Molecular mechanisms affecting placental formation in intrauterine growth-restricted (IUGR) pregnancies are not clearly understood. Since metastasis tumor antigens (MTAs) MTA1 and MTA2 promote cell proliferation and MTA3 suppresses it, we hypothesized that IUGR alters cell survival/cell death programs driven by placental MTAs. To induce IUGR, pregnant Sprague Dawley rats were given daily intraperitoneal injections of either saline or dexamethasone (0.4 mg/kg) starting from 14 days of gestation (dg) to either 19 dg or 21 dg...
December 8, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27920344/smad3-4-binding-to-promoter-ii-of-p450arom-so-as-to-regulate-aromatase-expression-in-endometriosis
#20
Juan Qu, Yuanyuan Zhu, Xiadi Wu, Juan Zheng, Zhen Hou, Yugui Cui, Yundong Mao, Jiayin Liu
Activin A can stimulate aromatase P450 (P450arom) expression in eutopic endometrial stromal cells (ESCs) of endometriosis by activin type I receptor-Smad pathway. In order to identify Smad3/4 binding to P450arom promoter II that mediates activin A response in ESCs, polymerase chain reaction (PCR) products of a serial truncated deletion of the P450arom promoter II region between -904 and +87 bp were inserted into the pGL3-basic vector to generate the promoter reporter plasmids. Luciferase reporter plasmids were cotransfected into cells with or without activin A (25 ng/mL)...
December 5, 2016: Reproductive Sciences
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