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Biomarker Insights

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https://www.readbyqxmd.com/read/29887728/cytokine-gene-polymorphisms-in-saudi-patients-with-atopic-dermatitis-a-case-control-study
#1
Ghaleb Bin Huraib, Fahad Al Harthi, Misbahul Arfin, Mohammed Al-Sugheyr, Sadaf Rizvi, Abdulrahaman Al-Asmari
The cause of atopic dermatitis (AD) is multifactorial and a number of genes including cytokines have been involved. We genotyped 315 subjects for polymorphisms in TNF-α and TNF-β and IL-10 genes. Patients had significantly higher frequency of GA genotype of TNF-α (-308 G/A) than healthy controls. Patients with AD and controls had similar distribution of A and G alleles. Genotype AA was found in 7.11% of controls while completely absent in cases. The frequencies of genotypes GG and AA of TNF-β (+252 A/G) polymorphism were higher whereas the frequency of genotype GA was significantly lower in patients than the controls...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29795976/impaired-cerebral-perfusion-in-multiple-sclerosis-relevance-of-endothelial-factors
#2
REVIEW
Lucia Monti, Lucia Morbidelli, Alessandro Rossi
Magnetic resonance imaging techniques measuring in vivo brain perfusion and integrity of the blood-brain barrier have developed rapidly in the past decade, resulting in a wide range of available methods. This review first discusses their principles, possible pitfalls, and potential for quantification and outlines clinical application in neurological disorders. Then, we focus on the endothelial cells of the blood-brain barrier, pointing out their contribution in regulating vascular tone by production of vasoactive substances...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29769800/the-therapeutic-potential-of-blocking-galectin-3-expression-in-acute-myocardial-infarction-and-mitigating-inflammation-of-infarct-region-a-clinical-outcome-based-translational-study
#3
Wassim Mosleh, Milind R Chaudhari, Swati Sonkawade, Supriya Mahajan, Charl Khalil, Kevin Frodey, Tanvi Shah, Suraj Dahal, Roshan Karki, Rujuta Katkar, W Matthijs Blankesteijn, Brian Page, Saraswati Pokharel, Minhyung Kim, Umesh C Sharma
Introduction: Increased galectin-3 is associated with ischemic cardiomyopathy, although its role in early remodeling post-myocardial infarction (MI) has not been fully elucidated. There are no data demonstrating that blocking galectin-3 expression would have an impact on the heart and that its relationship to remodeling is not simply an epiphenomenon. The direct association between galectin-3 and myocardial inflammation, dysfunction, and adverse cardiovascular outcomes post-MI was examined using clinical and translational studies...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29623000/lipidomic-profiling-of-plasma-and-erythrocytes-from-septic-patients-reveals-potential-biomarker-candidates
#4
Giovana Colozza Mecatti, Marcia Cristina Fernandes Messias, Rafaela Maria Sant'Anna Paiola, Célio Fernando Figueiredo Angolini, Ildenize Barbosa da Silva Cunha, Marcos Nogueira Eberlin, Patricia de Oliveira Carvalho
Background: Sepsis remains the primary cause of death from infection, despite advances in modern medicine. The identification of reliable diagnostic biomarkers for the early detection of this disease is critical and may reduce the mortality rate as it could allow early treatment. The purpose of this study was to describe the changes in the plasma and red cells blood lipidome profiling of patients diagnosed with sepsis and septic shock with the aim to identify potentially useful metabolic markers...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29568219/technical-validation-of-a-reverse-transcription-quantitative-polymerase-chain-reaction-in-vitro-diagnostic-test-for-the-determination-of-mir-31-3p-expression-levels-in-formalin-fixed-paraffin-embedded-metastatic-colorectal-cancer-tumor-specimens
#5
Lucas Ramon, Catherine David, Karine Fontaine, Elodie Lallet, Charles Marcaillou, Séverine Martin-Lannerée, Virginie Decaulne, Céline Vazart, Anne-Héloise Gélibert, Raouf Ben Abdelali, Jean-Marc Costa, Francis Rousseau, Raphaële Thiébaut, Larry Yost, Yann Gaston-Mathé
MiR-31-3p expression has been shown to be a predictive biomarker for response to anti-epithelial growth factor receptor therapy in patients with RAS wild-type metastatic colorectal cancer (mCRC). To aid in the quantification of miR-31-3p expression in formalin-fixed paraffin-embedded (FFPE) primary tumor samples from patients with mCRC, a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay was developed and validated. Assay development included the identification of a microRNA reference standard and the determination of an appropriate relative quantification cutoff for differentiating low versus high miR-31-3p expression...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29449777/a-homogeneous-time-resolved-fluorescence-immunoassay-method-for-the-measurement-of-compound-w
#6
Biao Huang, Huixin Yu, Jiandong Bao, Manda Zhang, William L Green, Sing-Yung Wu
Objective: Using compound W (a 3,3'-diiodothyronine sulfate [T2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29449776/nkx2-3-a-slippery-slope-from-development-through-inflammation-toward-hematopoietic-malignancies
#7
REVIEW
Dóra Vojkovics, Zoltán Kellermayer, Béla Kajtár, Giovanna Roncador, Áron Vincze, Péter Balogh
The development of peripheral lymphoid tissues from the mesoderm is the result of a complex convergence combining lymphohematopoietic differentiation with the local specification of nonhematopoietic mesenchymal components. Although the various transcriptional regulators with fate-determining effects in diversifying the mobile leukocyte subsets have been thoroughly studied and identified, the tissue-specific determinants promoting the regional differentiation of resident mesenchyme are less understood. Of these factors, various members of the NK-class Nkx paralogues have emerged as key regulators for the organogenesis of spleen and mucosal lymphoid tissues, and recent data have also indicated their involvement in various pathological events, including gut inflammation and hematopoietic malignancies...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29449775/prion-proteins-without-the-glycophosphatidylinositol-anchor-potential-biomarkers-in-neurodegenerative-diseases
#8
REVIEW
Valerija Kovač, Vladka Čurin Šerbec
Prion protein (PrP) is a biomolecule that is involved in neuronal signaling, myelinization, and the development of neurodegenerative diseases. In the cell, PrP is shed by the ADAM10 protease. This process generates PrP molecules that lack glycophosphatidylinositol anchor, and these molecules incorporate into toxic aggregates and neutralize toxic oligomers. Due to this dual role, these molecules are important biomarkers for neurodegenerative diseases. In this review, we present shed PrP as a potential biomarker, with a focus on PrP226*, which may be the main biomarker for predicting neurodegenerative diseases in humans...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29449774/reactive-oxygen-species-a-key-constituent-in-cancer-survival
#9
REVIEW
Seema Kumari, Anil Kumar Badana, Murali Mohan G, Shailender G, RamaRao Malla
Background: Cancer is one of the major heterogeneous disease with high morbidity and mortality with poor prognosis. Elevated levels of reactive oxygen species (ROS), alteration in redox balance, and deregulated redox signaling are common hallmarks of cancer progression and resistance to treatment. Mitochondria contribute mainly in the generation of ROS during oxidative phosphorylation. Elevated levels of ROS have been detected in cancers cells due to high metabolic activity, cellular signaling, peroxisomal activity, mitochondrial dysfunction, activation of oncogene, and increased enzymatic activity of oxidases, cyclooxygenases, lipoxygenases, and thymidine phosphorylases...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29371783/evaluation-of-a-serum-lung-cancer-biomarker-panel
#10
Peter J Mazzone, Xiao-Feng Wang, Xiaozhen Han, Humberto Choi, Meredith Seeley, Richard Scherer, Victoria Doseeva
Background: A panel of 3 serum proteins and 1 autoantibody has been developed to assist with the detection of lung cancer. We aimed to validate the accuracy of the biomarker panel in an independent test set and explore the impact of adding a fourth serum protein to the panel, as well as the impact of combining molecular and clinical variables. Methods: The training set of serum samples was purchased from commercially available biorepositories. The testing set was from a biorepository at the Cleveland Clinic...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29343939/kinetics-of-procalcitonin-in-pediatric-patients-on-extracorporeal-membrane-oxygenation
#11
REVIEW
Sara Bobillo, Javier Rodríguez-Fanjul, Anna Solé, Julio Moreno, Mònica Balaguer, Elisabeth Esteban, Francisco José Cambra, Iolanda Jordan
Objectives: To assess the kinetics of procalcitonin (PCT) and C-reactive protein (CRP) in pediatric patients who required extracorporeal membrane oxygenation (ECMO) and to analyze its relationship with morbidity and mortality. Patients and methods: Prospective observational study including pediatric patients who required ECMO. Both PCT and CRP were sequentially drawn before ECMO (P0) and until 72 hours after ECMO. Results: A total of 40 patients were recruited...
2018: Biomarker Insights
https://www.readbyqxmd.com/read/29308009/discovery-and-longitudinal-evaluation-of-candidate-biomarkers-for-ischaemic-stroke-by-mass-spectrometry-based-proteomics
#12
Marie Dagonnier, Ira Robin Cooke, Pierre Faou, Tara Kate Sidon, Helen Margaret Dewey, Geoffrey Alan Donnan, David William Howells
Application of acute therapies such as thrombolysis for ischaemic stroke (IS) is constrained because of diagnostic uncertainty and the dynamic nature of stroke biology. To investigate changes in blood proteins after stroke and as a result of thrombolysis treatment we performed label-free quantitative proteomics on serum samples using high-resolution mass spectrometry and long high-performance liquid chromatography gradient (5 hours) combined with a 50-cm column to optimise the peptide separation. We identified (false discovery rate [FDR]: 1%) and quantified a total of 574 protein groups from a total of 92 samples from 30 patients...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/29276374/plasma-cytochrome-c-detection-using-a-highly-sensitive-electrochemiluminescence-enzyme-linked-immunosorbent-assay
#13
Jeejabai Radhakrishnan, Rovi Origenes, Gina Littlejohn, Sanja Nikolich, Eunjung Choi, Sharon Smite, Lorissa Lamoureux, Alvin Baetiong, Manoj Shah, Raúl J Gazmuri
Background: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury. Methods and results: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28959121/inhaled-steroids-and-active-smoking-drive-chronic-obstructive-pulmonary-disease-symptoms-and-biomarkers-to-a-greater-degree-than-airflow-limitation
#14
Philip E Silkoff, Dave Singh, J Mark FitzGerald, Andreas Eich, Andrea Ludwig-Sengpiel, Geoffrey C Chupp, Vibeke Backer, Celeste Porsbjerg, Pierre-Olivier Girodet, Mark T Dransfield, Frederic Baribaud, Vedrana S Susulic, Matthew J Loza
RATIONALE: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease, and development of novel therapeutics requires an understanding of pathophysiologic phenotypes. OBJECTIVES: The purpose of the Airways Disease Endotyping for Personalized Therapeutics (ADEPT) study was to correlate clinical features and biomarkers with molecular characteristics in a well-profiled COPD cohort. METHODS: A total of 67 COPD subjects (forced expiratory volume in the first second of expiration [FEV1]: 45%-80% predicted) and 63 healthy smoking and nonsmoking controls underwent multiple assessments including patient questionnaires, lung function, and clinical biomarkers including fractional exhaled nitric oxide (FENO), induced sputum, and blood...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28932105/changes-in-alternative-splicing-as-pharmacodynamic-markers-for-sudemycin-d6
#15
Morgan Thurman, Jacob van Doorn, Barbara Danzer, Thomas R Webb, Stefan Stamm
OBJECTIVE: The aim of the study was to define pharmacodynamic markers for sudemycin D6, an experimental cancer drug that changes alternative splicing in human blood. METHODS: Blood samples from 12 donors were incubated with sudemycin D6 for up to 24 hours, and at several time points total RNA from lymphocytes was prepared and the pre-messenger RNA (mRNA) splicing patterns were analyzed with reverse transcription-polymerase chain reaction. RESULTS: Similar to immortalized cells, blood lymphocytes change alternative splicing due to sudemycin D6 treatment...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28890654/chondroitin-sulfate-inhibits-monocyte-chemoattractant-protein-1-release-from-3t3-l1-adipocytes-a-new-treatment-opportunity-for-obesity-related-inflammation
#16
Thomas V Stabler, Eulàlia Montell, Josep Vergés, Janet L Huebner, Virginia Byers Kraus
Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts were cultured and differentiated into adipocytes using an established protocol. Adipocytes were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release. At the same time, varying concentrations of chondroitin sulfate (CS) were added in a physiologically relevant range (10-200 µg/mL) to determine its impact on MCP-1 release...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28690396/prognostic-impact-of-urokinase-plasminogen-activator-receptor-expression-in-pancreatic-cancer-malignant-versus-stromal-cells
#17
REVIEW
Susanna Wl de Geus, Victor M Baart, Martin C Boonstra, Peter Jk Kuppen, Hendrica Ajm Prevoo, Andrew P Mazar, Bert A Bonsing, Hans Morreau, Cornelis Jh van de Velde, Alexander L Vahrmeijer, Cornelis Fm Sier
The urokinase plasminogen activator receptor (uPAR) has been proposed as a potential prognostic factor for various malignancies. The aim of this study is to assess the prognostic value of uPAR expression in neoplastic and stromal cells of patients with pancreatic adenocarcinoma. Urokinase plasminogen activator receptor expression was determined by immunohistochemistry in 122 pancreatic ductal adenocarcinomas. Kaplan-Meier and Cox regression analyses were used to determine the association with survival. Respectively 66%, 82% and 62% of patients with pancreatic cancer expressed uPAR in neoplastic cells, stromal, and in both combined...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28659713/making-meaningful-clinical-use-of-biomarkers
#18
REVIEW
Matthew J Selleck, Maheswari Senthil, Nathan R Wall
This review discusses the current state of biomarker discovery for the purposes of diagnostics and therapeutic monitoring. We underscore relevant challenges that have defined the gap between biomarker discovery and meaningful clinical use. We highlight recent advancements in and propose a way to think about future biomarker development.
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28615922/influence-of-circulating-endothelin-1-and-asymmetric-dimethylarginine-on-whole-brain-circulation-time-in-multiple-sclerosis
#19
Lucia Monti, Lucia Morbidelli, Lorenzo Bazzani, Alessandro Rossi
Blood-brain barrier (BBB) breakdown, inflammatory and immune cell activation, and chronic cerebral hypoperfusion are features of multiple sclerosis (MS). The aim is to determine the influence of endothelin-1 (ET1) and asymmetric dimethylarginine (ADMA) on cerebral circulation time (CCT) in patients with MS. In all, 64 patients with MS (39 relapsing-remitting [RR]-MS; 25 secondary progressive [SP]-MS subtype) and 37 controls (C) were studied. Cerebral circulation time was obtained by angiography. Plasmatic ET1 and ADMA were measured by enzyme-linked immunosorbent assay...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28615921/data-independent-acquisition-and-parallel-reaction-monitoring-mass-spectrometry-identification-of-serum-biomarkers-for-ovarian-cancer
#20
Navin Rauniyar, Gang Peng, TuKiet T Lam, Hongyu Zhao, Gil Mor, Kenneth R Williams
A data-independent acquisition (DIA)/parallel reaction monitoring (PRM) workflow was implemented to identify improved ovarian cancer biomarkers. Data-independent acquisition on ovarian cancer versus control sera and literature searches identified 50 biomarkers and indicated that apolipoprotein A-IV (ApoA-IV) is the most significantly differentially regulated protein. Parallel reaction monitoring with Targeted Ovarian Cancer Proteome Assay validated differential ApoA-IV expression and quantified 9 other biomarkers...
2017: Biomarker Insights
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