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Contrast Media & Molecular Imaging

Ulysse Gimenez, Hélène Lajous, Michèle El Atifi, Marie Bidart, Vincent Auboiroux, Pascal Henry Fries, François Berger, Hana Lahrech
Cellular MRI, which visualizes magnetically labelled cells (cells*), is an active research field for in vivo cell therapy and tracking. The simultaneous relaxation rate measurements (R2 *, R2 , R1 ) are the basis of a quantitative cellular MRI method proposed here. U937 cells were labelled with Molday ION Rhodamine B, a bi-functional superparamagnetic and fluorescent nanoparticle (U937*). U937* viability and proliferation were not affected in vitro. In vitro relaxometry was performed in a cell concentration range of [2...
October 21, 2016: Contrast Media & Molecular Imaging
Myoung Hyoun Kim, Chang Guhn Kim, Seul-Gi Kim, Dae-Weung Kim
The serine-aspartic acid-valine (SDV) peptide binds specifically to integrin αV β3 . In the present study, we successfully developed a TAMRA-GHEG-ECG-SDV peptide labeled with both Tc-99 m and TAMRA to target the integrin αV β3 of tumor cells; furthermore, we evaluated the diagnostic performance of Tc-99 m TAMRA-GHEG-ECG-SDV as a dual-modality imaging agent for tumor of the murine model. TAMRA-GHEG-ECG-SDV was synthesized using Fmoc solid-phase peptide synthesis. Radiolabeling of TAMRA-GHEG-ECG-SDV with Tc-99 m was done using ligand exchange methods...
October 14, 2016: Contrast Media & Molecular Imaging
S Manjura Hoque, Yuegao Huang, Emiliano Cocco, Samuel Maritim, Alessandro D Santin, Erik M Shapiro, Daniel Coman, Fahmeed Hyder
Ferrite-based ferri/superparamagnetic nanoparticles can be rapidly heated by an external alternating magnetic field (AMF) to induce tissue necrosis of the adjacent microenvironment, but in addition provide magnetic resonance imaging (MRI) contrast utilizing enhanced water relaxivity. Here we characterized nanoensembles of Fe-Co mixed spinel ferrites (i.e. Fex Co1-x Fe2 O4 , where x ranges from 0.2 to 0.8) synthesized by chemical co-precipitation. With nanoensembles of increasing Co content the saturation magnetization improved, while lattice parameter remained relatively constant...
September 22, 2016: Contrast Media & Molecular Imaging
Sayuan Liang, Karim Louchami, Hauke Kolster, Anna Jacobsen, Ying Zhang, Julian Thimm, Abdullah Sener, Joachim Thiem, Willy Malaisse, Tom Dresselaers, Uwe Himmelreich
The assessment of the β-cell mass in experimental models of diabetes and ultimately in patients is a hallmark to understand the relationship between reduced β-cell mass/function and the onset of diabetes. It has been shown before that the GLUT-2 transporter is highly expressed in both β-cells and hepatocytes and that D-mannoheptulose (DMH) has high uptake specificity for the GLUT-2 transporter. As 19-fluorine MRI has emerged as a new alternative method for MRI cell tracking because it provides potential non-invasive localization and quantification of labeled cells, the purpose of this project is to validate β-cell and pancreatic islet imaging by using fluorinated, GLUT-2 targeting mannoheptulose derivatives ((19) FMH) both in vivo and ex vivo...
September 14, 2016: Contrast Media & Molecular Imaging
Sashiprabha M Vithanarachchi, Casey D Foley, Sarah Trimpin, James R Ewing, Meser M Ali, Matthew J Allen
Reliable methods of imaging myelin are essential to investigate the causes of demyelination and to study drugs that promote remyelination. Myelin-specific compounds can be developed into imaging probes to detect myelin with various imaging techniques. The development of multimodal myelin-specific imaging probes enables the use of orthogonal imaging techniques to accurately visualize myelin content and validate experimental results. Here, we describe the synthesis and application of multimodal myelin-specific imaging agents for light microscopy and magnetic resonance imaging...
September 6, 2016: Contrast Media & Molecular Imaging
Tiantian Ye, Hefeng Zhang, Ge Chen, Lei Shang, Shujun Wang
Tumor metastatic lymph node mapping has been widely used to predict the metastatic spread of primary tumor and guide the lymph node dissection in clinical practice. In this research, a new near-infrared (NIR)-emitting low molecular weight heparin (LMWHEP)-modified Cy7-loaded nanoliposome (LMWHEP-NLips/Cy7) was developed and had the particle size of about 80 nm and the fluorescence intensity of about 2300, which is optimal for metastatic lymph node uptake and imaging. The NIR-emitting nanoliposomes were designed by LMWHEP coating on the surface of Cy7-loaded nanoliposome (NLips/Cy7) according to electrostatic attraction...
September 1, 2016: Contrast Media & Molecular Imaging
Mario Dentamaro, François Lux, Luce Vander Elst, Nicolas Dauguet, Sylvie Montante, Albert Moussaron, Carmen Burtea, Robert N Muller, Olivier Tillement, Sophie Laurent
Different studies on AGuIX nanoparticles have been achieved in the biomedical domain, showing that they allow us to combine multimodal and theranostic properties in oncology. The targeting of apoptotic cells presents a wide range of biomedical applications, including the monitoring of antitumoral therapy and the diagnosis of diseases related to this process, such as atherosclerosis, ischemia, chronic inflammation or autoimmune disorders. AGuIX nanoparticles functionalized with a peptide that recognizes apoptotic cells and with organic fluorophores were characterized by several physicochemical and biological methods such as HPLC, relaxometry and photon correlation spectroscopy, which attested to their potential as bimodal tracers detected by optical imaging and MRI...
September 2016: Contrast Media & Molecular Imaging
Weiping Jiang, Iris Yuwen Zhou, Lingyi Wen, Xin Zhou, Phillip Zhe Sun
Chemical exchange saturation transfer (CEST) MRI is sensitive to dilute labile protons and microenvironmental properties, augmenting routine relaxation-based MRI. Recent developments of quantitative CEST (qCEST) analysis such as omega plots and RF-power based ratiometric calculation have extended our ability to elucidate the underlying CEST system beyond the simplistic apparent CEST measurement. CEST MRI strongly varies with experimental factors, including the RF irradiation level and duration as well as repetition time and flip angle...
September 2016: Contrast Media & Molecular Imaging
Christel Vangestel, David Thomae, Jeroen Van Soom, Johan Ides, Leonie Wyffels, Patrick Pauwels, Sigrid Stroobants, Pieter Van der Veken, Viktor Magdolen, Jurgen Joossens, Koen Augustyns, Steven Staelens
Urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 are key players in cancer invasion and metastasis. Both uPA and PAI-1 have been described as prognostic biomarkers; however, non-invasive methods measuring uPA activity are lacking. We developed an indium-111 ((111) In)-labelled activity-based probe to image uPA activity in vivo by single photon emission computed tomography (SPECT). A DOTA-conjugated uPA inhibitor was synthesized and radiolabelled with (111) In ([(111) In]MICA-401), together with its inactive, hydrolysed form ([(111) In]MICA-402)...
August 24, 2016: Contrast Media & Molecular Imaging
Ping Dong, Huawei Cai, Lihong Chen, Yalun Li, Cen Yuan, Xiaoai Wu, Guohua Shen, Huijun Zhou, Wenjie Zhang, Lin Li
Neuropilin-1 (NRP-1) is overexpressed in several kinds of cancer cell and contributes to tumor aggressiveness. Recently, the arginine/lysine-rich peptide with C-terminal motifs (R/K)XX(R/K) indicated promising penetrating and transporting capability into NRP-1 positive cancer cells. In the present study, we describe a (131) I-labeled C-end rule motif peptide conjugate, Tyr-tLyp-1, for NRP-1 positive tumor targeting and imaging properties. Briefly, a truncated Lyp-1 peptide was designed to expose its C-end motif and conjugated to tyrosine for radiolabeling after structural modification...
August 15, 2016: Contrast Media & Molecular Imaging
Qiufang Liu, Min Zhou, Panli Li, Geng Ku, Gang Huang, Chun Li, Shaoli Song
Determining sentinel lymph node (SLN) status is critical to cancer staging and treatment decisions. Currently, in clinical practice, (99m) Tc-radiocolloid-mediated planar scintigraphy and single-photon emission computed tomography (SPECT) are used to guide the biopsy and resection of SLNs. Recently, an emerging technique that combines positron emission tomography (PET) and photoacoustic tomography (PAT; PET-PAT) may offer accurate information in detecting SLNs. Herein, we report a kind of (64) CuS-labeled nanoparticle ((64) CuS-NP) for the detection of SLNs with PET-PAT...
August 15, 2016: Contrast Media & Molecular Imaging
Anna A Krasilnikova, Anastasiya O Solovieva, Kristina E Trifonova, Konstantin A Brylev, Anton A Ivanov, Sung-Jin Kim, Michael A Shestopalov, Maria S Fufaeva, Alexander M Shestopalov, Yuri V Mironov, Alexander F Poveshchenko, Lidia V Shestopalova
The octahedral cluster compound Na2 H8 [{Re6 Se8 }(P(C2 H4 CONH2 )(C2 H4 COO)2 )6 ] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses...
August 4, 2016: Contrast Media & Molecular Imaging
Toshiko Harada, Yuko Nakamura, Kazuhide Sato, Tadanobu Nagaya, Peter L Choyke, Yasuyuki Seto, Hisataka Kobayashi
Optical fluorescence imaging has been developed as an aid to intraoperative diagnosis to improve surgical and endoscopic procedures. Compared with other intraoperative imaging methods, it is lower in cost, has a high safety margin, is portable and easy to use. γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) is a recently developed activatable fluorescence probe that emits strong fluorescence in the presence of the enzyme γ-glutamyl transpeptidase (GGT), which is overexpressed in many cancers, including ovarian cancer...
July 22, 2016: Contrast Media & Molecular Imaging
Nawal Tassali, Andrea Bianchi, François Lux, Gérard Raffard, Stéphane Sanchez, Olivier Tillement, Yannick Crémillieux
Idiopathic pulmonary fibrosis is a devastating disease. Animal models are critical to develop new diagnostic approaches. We investigate here whether the application of an ultra-short echo time MRI sequence combined with the intra-tracheal administration of Gd-based nanoparticles can help to visualize and characterize pulmonary fibrosis in mice. 21 mice were imaged. Treated mice were administered bleomycin. MRI was used for longitudinal detection of bleomycin-induced lung injury from Day 1 up to Day 60. On Day 30, all mice received nanoparticles and MR images were acquired...
July 11, 2016: Contrast Media & Molecular Imaging
Christina L Brunnquell, Reinier Hernandez, Stephen A Graves, Ivy Smit-Oistad, Robert J Nickles, Weibo Cai, M Elizabeth Meyerand, Masatoshi Suzuki
Manganese-enhanced magnetic resonance imaging (MRI) is an established neuroimaging method for signal enhancement, tract tracing, and functional studies in rodents. Along with the increasing availability of combined positron emission tomography (PET) and MRI scanners, the recent development of the positron-emitting isotope (52) Mn has prompted interest in the use of Mn(2+) as a dual-modality contrast agent. In this work, we characterized and compared the uptake of systemically delivered Mn(2+) and radioactive (52) Mn(2+) in the rat brain for MRI and PET, respectively...
July 11, 2016: Contrast Media & Molecular Imaging
Alessandro Silvestri, Vanessa Zambelli, Anna M Ferretti, Domenico Salerno, Giacomo Bellani, Laura Polito
The development of new molecules able to efficiently act as long-circulating computed tomography (CT) contrast agents is one of the most crucial topics in the biomedical field. In the last years, the chance to manipulate materials at the nano-size level gave new boost to this research, with the specific aim to design innovative nanoprobes. Gold nanoparticles (AuNPs) have showed unique X-rays attenuation properties which, combined with their easy surface functionalization, makes them ideal candidates for the next generation of contrast agents...
July 4, 2016: Contrast Media & Molecular Imaging
Claudia Sciallero, Emanuele Daglio, Andrea Trucco
Contrast-enhanced ultrasound with targeted microbubble contrast agents is an emerging technique for imaging biological processes at the molecular level. The accumulation of targeted microbubbles at tissue sites overexpressing specific molecular markers increases the backscattered signal for noninvasive evaluations of diseases. The aim of this preliminary study was to combine molecular imaging with an in vivo contrast agent quantification to support the early diagnosis of the pathology and to enhance the assessment of neoplastic tissues...
July 2016: Contrast Media & Molecular Imaging
Mitchell J Kingston, Diana M Perriman, Teresa Neeman, Paul N Smith, Alexandra L Webb
Barium sulfate and lead oxide contrast media are frequently used for cadaver-based angiography studies. These contrast media have not previously been compared to determine which is optimal for the visualisation and measurement of blood vessels. In this study, the lower limb vessels of 16 embalmed Wistar rats, and four sets of cannulae of known diameter, were injected with one of three different contrast agents (barium sulfate and resin, barium sulfate and gelatin, and lead oxide combined with milk powder). All were then scanned using micro-computed tomography (CT) angiography and 3-D reconstructions generated...
July 2016: Contrast Media & Molecular Imaging
Xing Yang, Xiaolei Song, Sangeeta Ray Banerjee, Yuguo Li, Youngjoo Byun, Guanshu Liu, Zaver M Bhujwalla, Martin G Pomper, Michael T McMahon
A series of intra-molecular hydrogen bonded imidazoles and related heterocyclic compounds were screened for their N-H chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) contrast properties. Of the compounds, imidazole-4,5-dicarboxamides (I45DCs) were found to provide the strongest contrast, with the contrast produced at a large chemical shift from water (7.8 ppm) and strongly dependent on pH. We have tested several probes based on this scaffold, and demonstrated that these probes could be applied for in vivo detection of kidney pH after intravenous administration...
July 2016: Contrast Media & Molecular Imaging
Francis T Hane, Peter S Smylie, Tao Li, Julia Ruberto, Krista Dowhos, Iain Ball, Boguslaw Tomanek, Brenton DeBoef, Mitchell S Albert
Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired (129) Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbit[6]uril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n = 5) at a concentration of 5 mM and 74% at 2...
July 2016: Contrast Media & Molecular Imaging
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