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Biomedical Materials

Yutaka Ikeda, Yukio Nagasaki
Oxidative stress caused by reactive oxygen species (ROS) occurs as events in which living tissues contact certain materials. These events include cell cultures and implantation of materials. Because of the high reactivity of ROS, they damage cells by oxidizing DNA, lipids, and proteins. Conversely, ROS also act as signaling molecules regulating cellular morphology. In particular, mitochondrial ROS are involved in the regulation of cellular physiology, including differentiation, autophagy, metabolic adaptation, apoptosis, and immunity...
March 16, 2018: Biomedical Materials
Chaoyu Liu, Chong Wang, Qilong Zhao, Xiaohua Li, Feiyue Xu, Xumei Yao, Min Wang
Electrospun fibrous scaffolds have been extensively used as cell-supporting matrices or delivery vehicles for various biomolecules in tissue engineering. Biodegradable scaffolds with tunable degradation behaviors are favorable for various resorbable tissue replacements. In nerve tissue engineering, delivery of growth factors (GFs) such as nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) from scaffolds can be used to promote peripheral nerve repair. In this study, using the established dual-source dual-power (DS-DP) electrospinning technique, bicomponent scaffolds incorporated with NGF and GDNF were designed and demonstrated as a strategy to develop scaffolds providing dual GF delivery...
March 14, 2018: Biomedical Materials
Ramkumar T Annamalai, Tapan Girish Naik, Haley Prout, Andrew J Putnam, Jan P Stegemann
Microtissues created from the protein fibrin and containing embedded cells can be used in modular tissue engineering approaches to create larger, hierarchical and complex tissue structures. In this paper we demonstrate an emulsification-based method for the production of such fibrin microtissues containing fibroblasts and endothelial cells and designed to promote tissue vascularization. Surfactants can be beneficial in the microtissue fabrication process to reduce aggregation and to facilitate recovery of microtissues from the emulsion, thereby increasing yield...
March 14, 2018: Biomedical Materials
Ander Izeta
No abstract text is available yet for this article.
March 13, 2018: Biomedical Materials
Zacnicte May, Tobias Fuehrman, Molly S Shoichet, Wolfram Tetzlaff, Jeff Biernaskie, Karim Fouad
letter of response.
March 13, 2018: Biomedical Materials
Anne Leferink, Diogo Santos, Clemens A van Blitterswijk, Lorenzo Moroni
When tissue engineering strategies rely on the combination of three-dimensional (3D) polymeric or ceramic scaffolds with cells to culture implantable tissue construct in vitro, it is desirable to monitor tissue growth and cell fate to be able to more rationally predict the quality and success of the construct upon implantation. Such a 3D construct is often referred to as a 'black-box' since the properties of the scaffolds material limit the applicability of most imaging modalities to assess important construct parameters...
March 8, 2018: Biomedical Materials
Xiudi Shi, Liwei Chen, Siwen Li, Xiaodan Sun, Fu-Zhai Cui, Hongmei Ma
Understanding the interaction between biomaterials and immune system has become more and more important. Mineralized collagen (MC) has the same chemical components and microstructures to the nature bone tissue, and which is considered as a better biomaterial for bone prostheses compared to hydroxyapatite (HA). However, there is little information about how MC affects inflammatory responses. In this study, we investigate the inflammatory responses to MC and HA by culturing RAW264.7 cells on their surfaces. We observed that MC increases CD206+ staining and IL-10 (M2 macrophages), whereas HA shows cells expressing more CD86 and secreting more TNF-α...
March 8, 2018: Biomedical Materials
Catherine Lambert, Devang Nijsure, Vincent Huynh, Ryan Wylie
Methods to reversibly control the chemical environment of hydrogels have application in three-dimensional (3D) cell culture to study cell proliferation, migration and differentiation in environments more representative of in vivo environments. Herein, we developed a method to temporally control the chemical environment of agarose hydrogels through non-covalent attachment of peptide motifs. Streptavidin-CGRGDS conjugates were immobilized in desthiobiotin-modified agarose hydrogels through the desthiobiotin-streptavidin interaction (KD 10-11 M)...
March 6, 2018: Biomedical Materials
Peizhen Duan, Juan Shen, Guohong Zou, Xu Xia, Bo Jin, Jiaxin Yu
Spherical porous hydroxyapatite (SHA)/graphene oxide (GO) composites with different GO (w/w) content of 16%, 40%, and 71% have been fabricated through a facile and controllable ultrasonic-assisted method at room temperature. The products were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), mechanical testing and biomimetic mineralization. Results showed SHA were covered by GO, and SHA/GO composites had an irregular surface with different degrees of wrinkles...
March 5, 2018: Biomedical Materials
Banani Kundu, Mohamed R Eltohamy, Vamsi K Yadavalli, Rui Luis Reis, Hae-Won Kim
The assembly of natural proteinaceous biopolymers into macro-scale architectures is of great importance in synthetic biology, soft-material science and regenerative therapy. The self-assembly of protein tends to be limited due to anisotropic interactions among protein molecules, poor solubility and stability. Here, we introduce a unique platform to self-immobilize diverse proteins (fibrous and globular, positively and negatively charged, low and high molecular weight) using silicon surfaces with pendant -NH2 groups via a facile one step diffusion limited aggregation (DLA) method...
February 28, 2018: Biomedical Materials
Jörn Hülsmann, Hug Aubin, Fabian Oberle, Nicolas Schuetterle, Shahbaz T Bandesha, Makoto Iijima, Artur Lichtenberg, Payam Akhyari
Whole-organ engineering - based on the functional repopulation of acellular whole-organ scaffolds derived from perfusion-based in toto decellularization of the specific organ system - is one of the most promising fields in tissue engineering. However, to date, we still have hardly any insights into the process of perfusion-based scaffold generation itself, with human-scale scaffolds usually obtained by adoption of small animal decellularization models, although those organs are of decreased biomass and potentially different biological characteristics...
February 21, 2018: Biomedical Materials
Marissa Gionet-Gonzales, Kent Leach
There is a critical need for strategies that effectively enhance cell viability and post-implantation performance in order to advance cell-based therapies. Spheroids, which are dense cellular aggregates, overcome many current limitations with transplanting individual cells. Compared to individual cells, the aggregation of cells into spheroids results in increased cell viability, together with enhanced proangiogenic, anti-inflammatory, and tissue-forming potential. Furthermore, the transplantation of cells using engineered materials enables localized delivery to the target site while providing an opportunity to guide cell fate in situ, resulting in improved therapeutic outcomes compared to systemic or localized injection...
February 20, 2018: Biomedical Materials
Raquel Maia, David Musson, Dorit Naot, Lucília Pereira da Silva, Ana R Bastos, João B Costa, Joaquim Miguel Oliveira, Vitor Manuel Correlo, Rui Luis Reis, Jillian Cornish
Bone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge about the interaction between osteoclasts and biomaterials. In this study, the ability of gellan gum and hydroxyapatite reinforced gellan gum spongy-like hydrogels to support osteoclastogenesis was investigated in vitro...
February 14, 2018: Biomedical Materials
Anh Tuan Nguyen, Sabrina Mattiassi, Manuela Loeblein, Eunice Chin, DongLiang Ma, Philippe Coquet, Virgile Viasnoff, E H T Teo, Eyleen L Goh, Evelyn Yim
Studies of electrical stimulation therapies for the treatment of neurological disorders, such as deep brain stimulation, have almost exclusively been performed using animal-models. However, because animal-models can only approximate human brain disorders, these studies should be supplemented with an in vitro human cell-culture based model to substantiate the results of animal-based studies and further investigate therapeutic benefit in humans. This study presents a novel approach to analyse the effect of electrical stimulation on the neurogenesis of patient-induced pluripotent stem cell (iPSC) derived neural progenitor cell (NPC) lines, in vitro using a 3D graphene scaffold system...
February 14, 2018: Biomedical Materials
Heyong Yin, Zexing Yan, Richard Bauer, Jiang Peng, Matthias Schieker, Michael Nerlich, Denitsa Docheva
Thermosensitive hydrogels have been studied for potential application as promising alternative cell carriers in cell-based regenerative therapies. In this study, a thermosensitive butane diisocyanate (BDI)-collagen hydrogel (BC hydrogel) was designed as injectable cell delivery carrier of tendon stem/progenitor cells (TSPCs) for tendon tissue engineering. We functionalized the BDI hydrogel with the addition of 20% (v/v) collagen I gel to obtain the thermosensitive BC hydrogel, which was then seeded with TSPCs derived from human Achilles tendons...
February 8, 2018: Biomedical Materials
Jakob M Townsend, Taylor A Zabel, Yi Feng, Jinxi Wang, Brian T Andrews, Randy Nudo, Cory Berkland, Michael S Detamore
In the treatment of severe traumatic brain injury (TBI), decompressive craniectomy is commonly used to remove a large portion of calvarial bone to allow unimpeded brain swelling. Hydrogels have the potential to revolutionize TBI treatment by permitting a single-surgical intervention, remaining pliable during brain swelling, and tuned to regenerate bone after swelling has subsided. With this motivation, our goal is to present a pliable material capable of regenerating calvarial bone across a critical size defect...
February 7, 2018: Biomedical Materials
Kassondra Hickey, Sarah E Stabenfeldt
Chemotaxis enables cellular communication and movement within the body. This review focuses on exploiting chemotaxis as a tool for repair of the central nervous system (CNS) damaged from injury and/or degenerative diseases. Chemokines and factors alone may initiate repair following CNS injury/disease, but exogenous administration may enhance repair and promote regeneration. This review will discuss critical chemotactic molecules and factors that may promote neural regeneration. Additionally, this review highlights how biomaterials can impact the presentation and delivery of chemokines and growth factors to alter the regenerative response...
February 7, 2018: Biomedical Materials
Kendell Pawelec, Jacob Koffler, Dena Shahriari, Angelica Rose Galvan, Mark Tuszynski, Jeff Sakamoto
In a previous study, we demonstrated a novel manufacturing approach to fabricate multi-channel scaffolds (MCS) for use in spinal cord injuries (SCI). In the present study, we extended similar materials processing technology to fabricate significantly longer (5X) porous poly caprolactone (PCL) MCS and evaluated their efficacy in 1 cm sciatic peripheral nerve injury (PNI) model. Due to the increase in MCS dimensions and the challenges that may arise in a longer nerve gap model, microstructural characterization involved MCS wall permeability to assess nutrient flow, topography to understand cell integration, and microstructural uniformity to evaluate the potential for homogeneous linear axon guidance...
February 7, 2018: Biomedical Materials
Dogeon Yoon, Dajeong Yoon, Hyuk-Jin Cha, Ji-Seon Lee, Wook Chun
The use of artificial dermis as a skin substitute represents a field of active study, as acellular dermal matrices from cadavers are susceptible to infection owing to their human origin. One such alternative dermal replacement scaffold, INSUREGRAF®, is derived primarily from extracellular matrix proteins such as collagen and elastin and has been clinically used to treat severe skin wounds such as burns. This scaffold has proven to be useful to minimize wound contraction and scar formation owing to its biocompatibility, interconnected pore structure, sufficient biodegradability, and suitable mechanical properties...
February 1, 2018: Biomedical Materials
Robert Chase Cornelison, Elisa Gonzalez-Rothi, Stacy L Porvasnik, Steven M Wellman, James H Park, David D Fuller, Christine E Schmidt
Spinal cord injury (SCI) affects a quarter million individuals in the United States, and there is currently no clinical treatment. Both fresh and acellular peripheral nerve grafts can induce spinal axon regeneration and support functional recovery in experimental injury models. Nonetheless, a scaffold that can be injected into a spinal contusion would be far less invasive to apply. We aimed to develop the first injectable acellular nerve graft for promoting repair after contusion SCI. 
 Approach: We report a method to enzymatically solubilize optimized acellular (OA) nerve - a decellularized peripheral nerve graft developed in our laboratory and currently used clinically - to obtain an injectable solution that undergoes thermal gelation under physiological conditions...
January 30, 2018: Biomedical Materials
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