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Biomedical Materials

Garima Tripathi, Yuki Sugiura, Kanji Tsuru, Kunio Ishikawa
β-tricalcium phosphate (β-TCP) is widely used in clinical applications as a bone substitute owing to its excellent tissue response and its ability to be replaced by new bone through a bone-remodeling process. However, it has disadvantages such as the rapid resorption in vivo and extremely high crystallinity. When Mg2+ is incorporated into β-TCP, not only the solubility of β-TCP decreases but also Mg2+ released from β-TCP attenuates osteoclast activity in vitro. Furthermore, a decrease in the crystallinity increases the biocompatibility of this bone substitute...
July 16, 2018: Biomedical Materials
Esra Cansever Mutlu, Anton Ficai, Denisa Ficai, Arzu Birinci Yildirim, Muhammet Yildirim, Faik Nuzhet Oktar, Arzu Demir
Electrospray is the promising technique to scale-up production of microparticles and nanoparticles. In this study, electrospraying was used in order to produce candidate biopatches (CPH) by using Chitosan, Poly(ethylene glycol) (PEG) and Hyaluronic acid (HA). Four different ratio of polymer blend compositions (CPH1, CPH2, CPH3 and CPH4) were tested by dissolving in 2% acetic acid solution (Ac.A.). HA amount in each blend was kept the same to designate the optimum surface with different chitosan/PEG ratios for electrospray process...
July 13, 2018: Biomedical Materials
Swati Midha, Sumit Kumar, Aarushi Sharma, Kulwinder Kaur, Xiaomeng Shi, Parichart Naruphontjirakul, Julian R Jones, Sourabh Ghosh
A major challenge in Bone tissue engineering is to develop patient-specific, defect-site specific grafts capable of triggering specific cell signaling pathways. We could programmably fabricate the 3D printed bone constructs via direct ink writing of silk-gelatin-bioactive glass (SF-G-BG) hybrids using two different compositions of melt-derived bioactive glasses (with and without strontium) and compared against commercial 45S5 Bioglass®. Physico-chemical characterization revealed that released ions from bioactive glasses inhibited the conformational change of Bombyx mori silk fibroin protein (from random coil to β-sheet conformation), affecting printability of the SF-G-BG ink...
July 11, 2018: Biomedical Materials
Ayse Selcen Alagoz, Jose Carlos Rodriguez-Cabello, Vasif Hasirci
A bone tissue replacement with relevant anatomical size requires production of 3D scaffolds, which in turn limit the mass transport of nutrients and oxygen to sustain cell survival. A viable vascular network is required to overcome this problem. However, this cannot be established immediately after the implantation of a scaffold. The aim of this study was to develop a 3D wet spun bone tissue engineering scaffold, coated with an elastin like recombinamers (ELR) peptide with endothelial cell attracting REDV sequence to promote early vascularization...
July 5, 2018: Biomedical Materials
Michael J McClure, Nicholas M Clark, Zvi Schwartz, Barbara D Boyan
Volumetric muscle loss is debilitating and involves extensive rehabilitation. One approach to accelerate healing, rehabilitation, and muscle function is to repair damaged skeletal muscle using regenerative medicine strategies. In sports medicine and orthopedics, a common clinical approach is to treat minor to severe musculoskeletal injuries with platelet-rich plasma (PRP) injections. While these types of treatments have become commonplace, there are limited data demonstrating their effectiveness. The goal of this study was to determine the effect of PRP on myoblast gene expression and protein production when incorporated into a polymer fiber...
July 3, 2018: Biomedical Materials
Irina Zurina, Anastasia Shpichka, Irina Saburina, Nastasia V Kosheleva, Anastasiya Gorkun, Ekaterina A Grebenik, Daria Kuznetsova, Deying Zhang, Yury Rochev, Denis Butnaru, Tatyana Zharikova, Elena Victorovna Istranova, Yuanyuan Zhang, Leonid Prokofievich Istranov, Peter S Timashev
Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling...
June 21, 2018: Biomedical Materials
Christiane Claaßen, Alexander Southan, Jana Grübel, Günter E M Tovar, Kirsten Borchers
Gelatin hydrogels are used as tissue engineering scaffolds and systems for controlled release due to their inherent biodegradability and biocompatibility. In this study gelatin methacryloyl (-acetyl) (GM/A) with various degrees of methacryloylation (DM) and methacryl-modified heparin (HepM) were cross-linked radically via thermal-redox initiation. Investigation of gel yields (79.4%-85.8%) and equilibrium degrees of swelling (EDS; 564.8%-750.3%) by an experimental design approach suggested interaction effects between applied HepM mass-fraction and DM of gelatin...
June 20, 2018: Biomedical Materials
Wenda Zhou, Jessica M Stukel, Abrar AlNiemi, Rebecca Kuntz Willits
For improved cell integration, tissue engineering scaffolds must be designed to degrade over time. Typically, the chemistry of scaffolds is modified to alter the degradation profile by using different hydrolytic or enzymatic sites within a material. It is more challenging, however, to fabricate self-assembling, injectable scaffolds that provide tunable degradation. Our laboratory has developed microgel-based scaffolds, where individual micron-sized hydrogels are crosslinked to make larger bulk scaffolds. The size of the individual microgels permits injection, and the microgels then self-assemble into a bulk structure and crosslink...
June 5, 2018: Biomedical Materials
Ana I Gonçalves, Margarida S Miranda, Márcia T Rodrigues, Rui Luis Reis, Manuela Gomes
The potential of magnetically assisted strategies within the remit of cell-based therapies is increasing and creates new opportunities in biomedical platforms and in the field of tissue engineering and regenerative medicine (TERM).
 Among the magnetic elements approached to build magnetically responsive strategies, superparamagnetic iron oxide nanoparticles (SPIONs) represent tunable and precise tools whose properties can be modelled for detection, diagnosis, targeting and therapy purposes. The most investigated clinical role of SPIONs is as contrast imaging agents for tracking and monitoring cells and tissues...
May 24, 2018: Biomedical Materials
Federico Vozzi, Federica Logrand, Manuela Cabiati, Claudia Cicione, Monica Boffito, Irene Carmagnola, Nicoletta Vitale, Manuele Gori, Mara Brancaccio, Silvia Del Ry, Dario Gastaldi, Emanuele Cattarinuzzi, Pasquale Vena, Alberto Rainer, Claudio Domenici, Gianluca Ciardelli, Susanna Sartori
Three-dimensional (3D) tissue models offer new tools in the study of diseases. In the case of the engineering of cardiac muscle, a realistic goal would be the design of a scaffold able to replicate the tissue-specific architecture, mechanical properties, and chemical composition, so that it recapitulates the main functions of the tissue. This work is focused on the design and preliminary biological validation of an innovative polyester urethane (PUR) scaffold mimicking cardiac tissue properties. The porous scaffold was fabricated by thermally induced phase separation (TIPS) from poly(ε-caprolactone) diol, 1,4-butanediisocyanate, and l-lysine ethyl ester...
July 3, 2018: Biomedical Materials
N Munir, A Callanan
Osteoarthritis is the leading cause of pain and disability worldwide. Despite treatment availability, fully functional and suitable long term treatments for large cartilage defects are yet to be sought. Cartilage tissue engineering provides an alternative treatment option with the potential of cartilage regeneration. Previously, scaffolds have been enhanced through coating with collagen type I, however, equal distribution of collagen and collagen penetration throughout the scaffold have been poor. Herein, this study aims to employ thermally induced phase separation to fabricate porous hybrid polycaprolactone (PCL)/collagen type I scaffolds, with equally distributed collagen type I throughout the scaffold...
June 27, 2018: Biomedical Materials
Krishna Madhavan, Winston Elliot, Yan Tan, Eric Monnet, Wei Tan
Failure of small-caliber grafts, used as bypass or reconstructive grafts in cardiovascular treatments, is often caused by thrombosis and stenosis. We have developed a multilayered, compliant graft with an electrospun heparin-encapsulated core and collagen-chitosan shell. Herein, the performances of acellular and cell-seeded grafts were evaluated in adult sheep for preclinical assessment. Allogeneic ovine marrow stroma cells (MSCs) were uniformly attached to the lumen of cell-seeded grafts. Interposition grafts were used for carotid arteries...
June 14, 2018: Biomedical Materials
Soheila Ali Akbari Ghavimi, Brittany N Allen, Jessica L Stromsdorfer, Jacob S Kramer, Xiaolei Li, Bret D Ulery
Due to the continually increasing clinical need to heal large bone defects, synthetic bone graft substitutes have become ever more necessary with calcium phosphates (CaP) widely used due to their similarity to the mineral component of bone. In this research, different concentrations of calcium ions (Ca2+ ), phosphate ions (Pi ), or their combination were provided to mesenchymal stem cells (MSCs) to evaluate their influence on proliferation and differentiation. The results suggest that 1-16 mM Ca2+ and 1-8 mM Pi is osteoinductive, but not cytotoxic...
June 14, 2018: Biomedical Materials
Di Zhang, Qiu-Wen Tan, Jing-Cong Luo, Qing Lv
Our previous study fabricated decellularized porcine muscle tissues (DPMTs) and demonstrated that DPMTs with few cell residues possess highly preserved protein components and good biocompatibility. In the physical state, skeletal muscle equips an abundant vascular network due to the vast demand of energy from aerobic metabolism. Vascular bioactive factors which are rich in skeletal muscle tissues may contribute to the angiogenic effect of DPMTs. However, implanting DPMTs in vivo in a less invasive way is unfeasible...
June 11, 2018: Biomedical Materials
Donghai Li, Liu Huifang, Jinhai Zhao, Zhouyuan Yang, Xiaowei Xie, Zhun Wei, Dongzhe Li, Pengde Kang
Hydroxyapatite (HA) is a commonly used biomaterial in bone-tissue engineering, but pure HA is deficient in osteoinduction. In this study, we fabricated scaffolds of lithium-doped HA (Li-HA) and assess the bone generation enhancement of Li-HA scaffolds seeded with hypoxia-preconditioned bone-marrow mesenchymal stem cells (BMMSCs). We found that 1.5%Li-HA obtained optimal cell proliferation activity in vitro. In an in vivo study, Li-HA/BMSCs enhanced new bone formation, reducing the GSK-3β and increasing the β-catenin, but the angiogenic effect was not modified significantly...
June 6, 2018: Biomedical Materials
E A Grebenik, V D Grinchenko, S N Churbanov, N V Minaev, B S Shavkuta, P A Melnikov, D V Butnaru, Yu A Rochev, V N Bagratashvili, P S Timashev
This study was aimed to design and characterise hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8 × 8 × 2.5 mm and ladder-like pores (∼700 μm in width). Hyaluronic acid-coated polylactide microparticles (∼100 μm in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator...
June 6, 2018: Biomedical Materials
Tobias Führmann, Molly S Shoichet
No abstract text is available yet for this article.
June 4, 2018: Biomedical Materials
Christopher D Johnson, Anthony R D'Amato, Devan L Puhl, Douglas M Wich, Amanda Vesperman, Ryan J Gilbert
Aligned, electrospun fiber scaffolds provide topographical guidance for regenerating neurons and glia after central nervous system injury. To date, no study has explored how fiber surface nanotopography affects astrocyte response to fibrous scaffolds. Astrocytes play important roles in the glial scar, the blood brain barrier, and in maintaining homeostasis in the central nervous system. In this study, electrospun poly L-lactic acid fibers were engineered with smooth, pitted, or divoted surface nanotopography...
June 4, 2018: Biomedical Materials
Katarzyna Krukiewicz, Magdalena Chudy, Catalina Vallejo-Giraldo, Małgorzata Skorupa, Daria Więcławska, Roman Turczyn, Manus Biggs
Electrically conducting polymer formulations have emerged as promising approaches for the development of interfaces and scaffolds in neural engineering, facilitating the development of physicochemically modified constructs capable of cell stimulation through electrical and ionic charge transfer. In particular, topographically functionalized or neuromorphic materials are able to guide the growth of axons and promote enhanced interfacing with neuroelectrodes in vitro. In this study, we present a novel method for the formation of conducting polymer/gold assemblies via a combinational sputter and spin coating technique...
June 4, 2018: Biomedical Materials
Annemarie Schönfeld, Mihai Constantinescu, Kirsten Peters, Martin Frenz
BACKGROUND AND OBJECTIVE: Electrospun meshes have been extensively investigated for tissue engineering and drug delivery. The application of this technology is of interest for laser-assisted vascular anastomosis (LAVA) due to the possibility to bind and stabilize macromolecules in fibers. MATERIALS AND METHODS: We prepared bovine serum albumin (BSA) blend microfibers from the auxiliary proteins polyethylene oxide (PEO), polycaprolactone (PCL), polyvinyl alcohol (PVA) and gelatin...
May 29, 2018: Biomedical Materials
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