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Future Microbiology

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https://www.readbyqxmd.com/read/29469615/enhanced-photodynamic-therapy-using-light-fractionation-against-s-mutans-biofilm-type-i-and-type-ii-mechanism
#1
Lama Misba, Asad U Khan
AIM: The objective of the study was to look the efficacy of fractionated light against Streptococcus mutans biofilm. MATERIALS & METHODS: Antibiofilm assays (crystal violet, congo red), electron microscopic, confocal and spectroscopic studies were performed to check the effect of fractionated light. RESULTS: 6-6.5 log10 reduction of planktonic and 3.6-4.2 log10 reduction in biofilm were observed after irradiation with fractionated as compared with continuous light...
February 22, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29469610/comparative-analysis-between-biofilm-formation-and-gene-expression-in-staphylococcus-epidermidis-isolates
#2
Ana I Freitas, Nathalie Lopes, Fernando Oliveira, Susana Brás, Ângela França, Carlos Vasconcelos, Manuel Vilanova, Nuno Cerca
AIM: To understand the relationship between ica, aap and bhp gene expression and the implications in biofilm formation in selected clinical and commensal Staphylococcus epidermidis isolates. MATERIAL & METHODS: Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, biofilm spatial organization and expression of biofilm-related genes. RESULTS: On polysaccharide intercellular adhesin-dependent biofilms, aap and bhp contributions for the biofilm growth were negligible, despite very high levels of expression...
February 22, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29469596/genome-scale-metabolic-models-as-platforms-for-identification-of-novel-genes-as-antimicrobial-drug-targets
#3
Bashir Sajo Mienda, Rabiu Salihu, Aliyu Adamu, Shehu Idris
The growing number of multidrug-resistant pathogenic bacteria is becoming a world leading challenge for the scientific community and for public health. However, advances in high-throughput technologies and whole-genome sequencing of bacterial pathogens make the construction of bacterial genome-scale metabolic models (GEMs) increasingly realistic. The use of GEMs as an alternative platforms will expedite identification of novel unconditionally essential genes and enzymes of target organisms with existing and forthcoming GEMs...
February 22, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29464977/how-combined-multicomparative-proteomic-approaches-can-improve-the-understanding-of-quinolone-resistance-in-salmonella-typhimurium
#4
Susana Correia, Michel Hébraud, Patrícia Poeta, José L Capelo, Gilberto Igrejas
No abstract text is available yet for this article.
February 21, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29464970/comparative-evaluation-of-genexpert-mtb-rif-and-multiplex-pcr-targeting-mpb64-and-is6110-for-the-diagnosis-of-pleural-tb
#5
Suman Sharma, Bhawna Dahiya, Vishnubhatla Sreenivas, Netrapal Singh, Ankush Raj, Abhishek Sheoran, Aparna Yadav, Krishna B Gupta, Promod K Mehta
AIM: Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test. METHODS: We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (n = 78) of pleural TB patients and non-TB controls. RESULTS: The sensitivities of 89.6 and 33.3%, and specificities of 96...
February 21, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29464969/clostridioides-clostridium-difficile-infection-current-and-alternative-therapeutic-strategies
#6
Fernando Gil, Iván L Calderón, Juan A Fuentes, Daniel Paredes-Sabja
Clostridioides difficile (C. difficile) has become a pathogen of worldwide importance considering that epidemic strains are disseminated in hospitals of several countries, where community-acquired infections act as a constant source of new C. difficile strains into hospitals. Despite the advances in the treatment of infections, more effective therapies against C. difficile are needed but, at the same time, these therapies should be less harmful to the resident gastrointestinal microbiota. The purpose of this review is to present a description of issues associated to C...
February 21, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29464968/forecasting-the-size-and-peak-of-cholera-epidemic-in-yemen-2017
#7
Hiroshi Nishiura, Shinya Tsuzuki, Yusuke Asai
No abstract text is available yet for this article.
February 21, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29464962/antifungal-activity-of-fluconazole-loaded-natural-rubber-latex-against-candida-albicans
#8
Mônica Yonashiro Marcelino, Felipe Azevedo Borges, Ana Flávia Martins Costa, Junya de Lacorte Singulani, Nathan Vinícius Ribeiro, Caroline Barcelos Costa-Orlandi, Bruna Cambraia Garms, Maria José Soares Mendes-Giannini, Rondinelli Donizetti Herculano, Ana Marisa Fusco-Almeida
AIM: This work aimed to produce a membrane based on fluconazole-loaded natural rubber latex (NRL), and study their interaction, drug release and antifungal susceptibility against Candida albicans. MATERIALS & METHODS: Fluconazole-loaded NRL membrane was obtained by casting method. RESULTS: The Fourier Transform Infrared Spectroscopy showed no modifications either in NRL or fluconazole after the incorporation. Mechanical test presented low Young's modulus and high strain, indicating the membranes have sufficient elasticity for biomedical application...
February 21, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29446650/methods-for-culturing-anaerobes-from-human-specimen
#9
Elodie Guilhot, Saber Khelaifia, Bernard La Scola, Didier Raoult, Grégory Dubourg
Anaerobes represent the dominating population in the human gut microbiota and play a key role in gut homeostasis. In addition, several anaerobes are now considered as probiotics and they remain essential to several processes in the field of biotechnology. With the implementation of MALDI-TOF MS in routine laboratories, anaerobes are no longer neglected in clinical microbiology, as their identification is made easy. However, the isolation and identification of anaerobic bacteria, remains time consuming, fastidious and costly...
February 15, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29446642/revealing-the-dual-role-of-gallic-acid-in-modulating-ampicillin-sensitivity-of-pseudomonas-aeruginosa-biofilms
#10
Rekha Yamini Kosuru, Md Aashique, Aisha Fathima, Amrita Roy, Soumen Bera
AIM: To understand the effects of gallic acid (GA) on ampicillin (Amp) sensitive or resistant strain of Pseudomonas sp. and also in modulating the corresponding biofilms. METHODOLOGY: The cell viability was determined by broth dilution, dry weight and CFU assays. Biofilm formation was measured by crystal violet assay while oxygen consumption rate was measured to verify the metabolic status of the cells. The membrane damage and drug efflux/accumulation were studied by fluorimetric assays...
February 15, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29441824/green-synthesis-of-silver-nanoparticles-combined-to-calcium-glycerophosphate-antimicrobial-and-antibiofilm-activities
#11
José As Souza, Debora B Barbosa, Andresa A Berretta, Jackeline G do Amaral, Luiz F Gorup, Francisco N de Souza Neto, Renan A Fernandes, Gabriela L Fernandes, Emerson R Camargo, Alessandra M Agostinho, Alberto Cb Delbem
AIM: To synthesize, characterize and evaluate the antimicrobial and antibiofilm activities of novel nanocomposites containing silver nanoparticles (AgNPs) associated or not to β-calcium glycerophosphate. MATERIALS & METHODS: These nanocomposites were produced through a 'green' route using extracts of different parts of pomegranate. Antimicrobial and antibiofilm properties against Candida albicans and Streptococcus mutans were determined by the minimum bactericidal/fungicidal concentration and biofilm density after treatments...
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29441822/role-and-regulation-of-the-orphan-apha-protein-of-quorum-sensing-in-pathogenic-vibrios
#12
Renfei Lu, George Osei-Adjei, Xinxiang Huang, Yiquan Zhang
Quorum sensing (QS), a cell-to-cell communication process, is widely distributed in the bacterial kingdom. Bacteria use QS to control gene expression in response to cell density by detecting the signal molecules called autoinducers. AphA protein is the master QS regulator of vibrios operating at low cell density. It regulates the expression of a variety of genes, especially those encoding virulence factors, flagella/motility and biofilm formation. The role and regulation of AphA in vibrios, especially in human pathogenic vibrios, are summarized in this review...
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29441819/hoisted-by-their-own-petard-do-microbial-enzymes-hold-the-solution-to-treating-and-preventing-biofilm-infections
#13
Brendan D Snarr, P Lynne Howell, Donald C Sheppard
No abstract text is available yet for this article.
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29441802/phage-derived-lysins-as-therapeutic-agents-against-multidrug-resistant-enterococcus-faecalis
#14
Jingmin Gu, Hengyu Xi, Mengjun Cheng, Wenyu Han
No abstract text is available yet for this article.
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29441801/characterizing-the-meningococcal-glycointeractome-what-is-new
#15
Kate L Seib, Michael P Jennings, Christopher J Day
No abstract text is available yet for this article.
February 14, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29436239/the-mrna-export-factor-sac3-maintains-nuclear-homeostasis-and-regulates-cytoskeleton-organization-in-candida-albicans
#16
Chenpeng Xiao, Qilin Yu, Bing Zhang, Jianrong Li, Dan Zhang, Mingchun Li
AIM: In eukaryotes, the nuclear export of mRNAs is essential for gene expression and regulations of numerous cellular processes. This study aimed to identify the mRNA export factor Sac3 in Candida albicans. MATERIALS & METHODS: A sac3Δ/Δ mutant was obtained using PCR-mediated homologous recombination. RESULTS: Disruption of SAC3 caused abnormal accumulation of mRNA in the nuclei. Further investigations revealed that sac3Δ/Δ mutant exhibited a severely growth defect, which was related to abnormal aggregation of microtubules...
February 13, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29424247/corrigendum
#17
(no author information available yet)
No abstract text is available yet for this article.
February 9, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29327947/secnidazole-next-generation-antimicrobial-agent-for-bacterial-vaginosis-treatment
#18
Paul Nyirjesy, Jane R Schwebke
Secnidazole is a next-generation 5-nitroimidazole approved for more than three decades in Europe, Asia, South America and Africa and recently in the USA as a single-dose (2 g) treatment of bacterial vaginosis (BV). Secnidazole is characterized by potent in vitro antimicrobial activity against BV-associated pathogens, as well as prolonged terminal elimination half-life and systemic exposure. These characteristics form the basis of effective and safe treatment of BV with a 2-g single-dose secnidazole regimen, which was recently confirmed in double-blind, placebo-controlled clinical trials conducted in the USA...
January 12, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29322818/evolution-of-ph-buffers-and-water-homeostasis-in-eukaryotes-homology-between-humans-and-acanthamoeba-proteins
#19
Abdul M Baig, R Zohaib, S Tariq, H R Ahmad
AIM: This study intended to trace the evolution of acid-base buffers and water homeostasis in eukaryotes. Acanthamoeba castellanii  was selected as a model unicellular eukaryote for this purpose. Homologies of proteins involved in pH and water regulatory mechanisms at cellular levels were compared between humans and A. castellanii. MATERIALS & METHODS: Amino acid sequence homology, structural homology, 3D modeling and docking prediction were done to show the extent of similarities between carbonic anhydrase 1 (CA1), aquaporin (AQP), band-3 protein and H+ pump...
January 11, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29320882/the-impact-of-influenza-virus-infection-in-pregnancy
#20
Lucy K Somerville, Kerri Basile, Dominic E Dwyer, Jen Kok
Data from previous seasonal epidemics and pandemics have confirmed that pregnant women are at increased risk for severe influenza virus infection. Complications including fetal loss, higher rates of hospitalization and maternal death are most notable during the late gestational period. Antiviral therapy and influenza vaccination are recommended in pregnant women as both are effective and safe. This review discusses the epidemiology, pathophysiology, treatment and prevention of influenza virus infection in pregnancy, with a focus on recent developments...
January 11, 2018: Future Microbiology
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