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Future Microbiology

Haili Wang, Wenliang Li, Lixiao Gu, Xiaofang Gao, Bin Ni, Haiteng Deng, Ruifu Yang, Yanping Han
AIM: To isolate and characterize the two phenotypically distinct subpopulations from Klebsiella pneumoniae clonal cultures grown in the simulate microgravity environment. MATERIALS & METHODS: Here clonal culture of K. pneumoniae strain ATCC BAA-1705 was grown within a vertically rotating wall vessel bioreactor. Microscopic, colony staining, biofilm assays and quantitative proteomics were used to define the features of subpopulations. RESULTS: Two subpopulations were isolated based on colony appearance and bacterial morphology and indicated the different capability of biofilm formation and antibiotics resistance...
August 17, 2017: Future Microbiology
Ming-Yi Wang, Xiao-Fei Liu, Jun Xia, Yu Li, Jian-Li Geng, Cheng-Jin Hu
AIM: To characterize the roles of VvhA in host's acquired immune response to Vibrio vulnificus infection. MATERIALS & METHODS: The recombinant VvhA fusion protein was used to immunize mice and the anti-VvhA polyclonal antibody was produced in vivo for prophylactic and therapeutic efficacy assay. The roles of VvhA in T helper (Th) cells differentiation were analyzed by vvhA-deleted mutant during the early phase of infection, while the ratio of Th2 and T follicular helper (Tfh) cells were examined in VvhA immunization...
August 17, 2017: Future Microbiology
Mark H Wilcox, Natalia Dmitrieva, Ana Cristina Gales, Irina Petukhova, Suleiman Al-Obeid, Flavia Rossi, Joseph M Blondeau
Inappropriate use and overuse of antibiotics are among the most important factors in resistance development, and effective antibiotic stewardship measures are needed to optimize outcomes. Selection of appropriate antimicrobials relies on accurate and timely antimicrobial susceptibility testing. However, the availability of clinical breakpoints and in vitro susceptibility testing often lags behind regulatory approval by several years for new antimicrobials. A Working Group of clinical/medical microbiologists from Brazil, Canada, Mexico, Saudi Arabia, Russia and the UK recently examined issues surrounding antimicrobial susceptibility testing for novel antibiotics...
August 16, 2017: Future Microbiology
Shih-Chun Yang, Feng-Lin Yen, Pei-Wen Wang, Ibrahim A Aljuffali, Yi-Han Weng, Chih-Hua Tseng, Jia-You Fang
AIM: Naphtho[1,2-b]furan-4,5-dione (N12D) and naphtho[2,3-b]furan-4,9-dione (N23D) are furanonaphthoquinone derivatives from natural resources. We examined the antimicrobial activity of N12D and N23D against drug-resistant Staphylococcus aureus. MATERIALS & METHODS: Minimum inhibitory concentration, minimum bactericidal concentration, bacterial viability and agar diffusion assay were conducted against methicillin-resistant S. aureus (MRSA) and clinical isolates of vancomycin-resistant S...
August 11, 2017: Future Microbiology
Defu Zhang, Zhe Yin, Yuzong Zhao, Jiao Feng, Xiaoyuan Jiang, Zhe Zhan, Weili Wu, Weijun Chen, Jinglin Wang, Jianrong Li, Dongsheng Zhou
AIM: This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae 1220 carrying bla CTX-M-14 and qnrB4. MATERIALS & METHODS: Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia coli through conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK. RESULTS: p1220-CTXM was an IncFIIK plasmid genetically closely related to pKP048 and carried resistance markers including bla CTX-M-14, bla DHA-1, qnrB4, sul1 and qacEΔ1, all of which were harbored in a 35...
August 11, 2017: Future Microbiology
Jie Ren, Yu Sang, Ran Qin, Zhongli Cui, Yu-Feng Yao
AIM: Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood. METHODS: We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium. RESULTS: It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3...
August 10, 2017: Future Microbiology
Wuyu Mao, Hexin Xie
No abstract text is available yet for this article.
August 10, 2017: Future Microbiology
Dijana Varda Brkic, Irina Pristas, Ivan Cipris, Marko Jelic, Iva Butic, Arjana Tambic Andrasevic
AIM: We report the first outbreak caused by Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae sequence type 258 (ST 258) clone that occurred in Croatia from May to December 2012. MATERIALS & METHODS: 23 carbapenem-resistant K. pneumoniae isolates were recovered from clinical and screening specimens of 12 patients hospitalized in a regional hospital. The first isolates from the 12 patients were typed using pulsed-field gel electrophoresis and multilocus sequence typing...
August 10, 2017: Future Microbiology
Faizan A Sadiq, Steve Flint, YanJun Li, Kai Ou, Lei Yuan, Guo Qing He
Phenotypic changes or phase variation within biofilms is an important feature of bacterial dormant life. Enhanced resistance to antimicrobials is one of the distinct features displayed by a fraction of cells within biofilms. It is believed that persisters are mainly responsible for this phenotypic heterogeneity. However, there is still an unresolved debate on the formation of persisters. In this short review, we highlight all known genomic and proteomic changes encountered by bacterial cells within biofilms...
August 7, 2017: Future Microbiology
Rebecca A Hall
No abstract text is available yet for this article.
August 2, 2017: Future Microbiology
Charles Darkoh, Magdalena Deaton, Herbert L DuPont
Clostridium difficile infection (CDI) is a major public health problem worldwide. Treatment has become complicated due to the emergence of strains with increased toxigenicity and sporulation rate, together with rampant antibiotics use that disrupts colonization resistance of the colonic microbiota. As a result, there is a critical need for nonantibiotic treatments. Therapies based on inhibiting the toxins, bacterial structures responsible for colonization, virulence and restoration of the gut microbiota are the most important nonantibiotic targets to combat CDI...
July 31, 2017: Future Microbiology
Nazario D Rivera-Chaparro, Michael Cohen-Wolkowiez, Rachel G Greenberg
Antibiotics are often used in neonates despite the absence of relevant dosing information in drug labels. For neonatal dosing, clinicians must extrapolate data from studies for adults and older children, who have strikingly different physiologies. As a result, dosing extrapolation can lead to increased toxicity or efficacy failures in neonates. Driven by these differences and recent legislation mandating the study of drugs in children and neonates, an increasing number of pharmacokinetic studies of antibiotics are being performed in neonates...
July 31, 2017: Future Microbiology
Luis Eduardo López-Cortés, Marina de Cueto, Jesús Rodríguez-Baño
No abstract text is available yet for this article.
July 31, 2017: Future Microbiology
Isabelle Bekeredjian-Ding
No abstract text is available yet for this article.
July 27, 2017: Future Microbiology
Michael Otto
No abstract text is available yet for this article.
July 27, 2017: Future Microbiology
Angela M Costa, Filipe J Mergulhão, Romain Briandet, Nuno F Azevedo
Multispecies biofilms represent the dominant mode of life for the vast majority of microorganisms. Bacterial spatial localization in such biostructures governs ecological interactions between different populations and triggers the overall community functions. Here, we discuss the pros and cons of fluorescence-based techniques used to decipher bacterial species patterns in biofilms at single cell level, including fluorescence in situ hybridization and the use of genetically modified bacteria that express fluorescent proteins, reporting the significant improvements of those techniques...
July 26, 2017: Future Microbiology
Massimo Fantoni, Rita Murri, Roberto Cauda
No abstract text is available yet for this article.
July 26, 2017: Future Microbiology
Yang Xu, Yong Niu, Fengjun Sun, Ying Yang, Wenbo Luo, Zhe Wang
AIM: This study aims to characterize a multidrug-resistant (MDR) plasmid p12969-2 coexistent with the previously reported one p12969-DIM in clinical Pseudomonas putida. MATERIALS & METHODS: The complete sequence of p12969-2 was determined using next-generation sequencing technology. RESULTS: p12969-2 contains a 29.2 kb MDR region, which carries In127 harboring three resistance genes aadA2, qacED1 and sul1. The MDR region is derived from the connection of Tn5041D and Tn5045, which is facilitated by two copies of miniature inverted-repeat transposable element...
June 29, 2017: Future Microbiology
Nathalie Rolhion, Pascale Cossart
The opportunistic intracellular bacterial pathogen Listeria monocytogenes has in 30 years emerged as an exceptional bacterial model system in infection biology. Research on this bacterium has provided considerable insight into how pathogenic bacteria adapt to mammalian hosts, invade eukaryotic cells, move intracellularly, interfere with host cell functions and disseminate within tissues. It also contributed to unveil features of normal host cell pathways and unsuspected functions of previously known cellular proteins...
June 12, 2017: Future Microbiology
Jimmy Tri Trinh, Lanying Zeng
No abstract text is available yet for this article.
June 12, 2017: Future Microbiology
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