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Future Microbiology

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https://www.readbyqxmd.com/read/28608712/nf-gh-a-glycosidase-secreted-by-naegleria-fowleri-causes-mucin-degradation-an-in-vitro-and-in-vivo-study
#1
Moisés Martínez-Castillo, Rosa Elena Cárdenas-Guerra, Rossana Arroyo, Anjan Debnath, Mario Alberto Rodríguez, Myrna Sabanero, Fernando Flores-Sánchez, Fernando Navarro-Garcia, Jesús Serrano-Luna, Mineko Shibayama
AIM: The aim of this work was to identify, characterize and evaluate the pathogenic role of mucinolytic activity released by Naegleria fowleri. MATERIALS & METHODS: Zymograms, protease inhibitors, anion exchange chromatography, MALDI-TOF-MS, enzymatic assays, Western blot, and confocal microscopy were used to identify and characterize a secreted mucinase; inhibition assays using antibodies, dot-blots and mouse survival tests were used to evaluate the mucinase as a virulence factor...
June 13, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604108/how-the-study-of-listeria-monocytogenes-has-led-to-new-concepts-in-biology
#2
Nathalie Rolhion, Pascale Cossart
The opportunistic intracellular bacterial pathogen Listeria monocytogenes has in 30 years emerged as an exceptional bacterial model system in infection biology. Research on this bacterium has provided considerable insight into how pathogenic bacteria adapt to mammalian hosts, invade eukaryotic cells, move intracellularly, interfere with host cell functions and disseminate within tissues. It also contributed to unveil features of normal host cell pathways and unsuspected functions of previously known cellular proteins...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604103/virus-interactions-cooperation-or-competition
#3
Jimmy Tri Trinh, Lanying Zeng
No abstract text is available yet for this article.
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604102/antimicrobial-resistance-man-s-best-friend-what-they-give-to-us-we-might-be-giving-right-back
#4
Joseph M Blondeau
No abstract text is available yet for this article.
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604101/post-dieting-weight-gain-the-role-of-persistent-microbiome-changes
#5
Christoph A Thaiss, Hagit Shapiro, Eran Elinav
No abstract text is available yet for this article.
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604100/molecular-epidemiology-of-carbapenem-resistant-acinetobacter-baumannii-in-greece-an-extended-review-2000-2015
#6
Theodoros Karampatakis, Charalampos Antachopoulos, Athanassios Tsakris, Emmanuel Roilides
Carbapenem-resistant Acinetobacter baumannii (CRAB) is endemic in Greece. CRAB initially emerged in 2000 and since then, carbapenemases still have a crucial role in CRAB appearance, except for a few cases resulting from efflux pump or outer-membrane protein mechanisms. OXA-type carbapenemases present the highest prevalence worldwide and bla OXA-23-like and bla OXA-58-like are the most important genes found; VIM-yielding CRAB have also been detected, while a single CRAB isolate producing NDM has quite recently emerged in Greece...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604069/novel-human-metapneumovirus-with-a-180-nucleotide-duplication-in-the-g-gene
#7
María Piñana, Jorgina Vila, Laura Gimferrer, María Valls, Cristina Andrés, María Gema Codina, Javier Ramón, María Carmen Martín, Francisco Fuentes, Rosario Saiz, Pilar Alcubilla, Carlos Rodrigo, Tomàs Pumarola, Andrés Antón
AIM: To describe the circulation, genetic diversity and clinical features of human metapneumovirus (HMPV) in pediatric patients that attended the Hospital Universitari Vall d'Hebron, Spain from 2014 to 2016. MATERIALS & METHODS: Partial G gene was sequenced from laboratory-confirmed HMPV respiratory specimens for subsequent phylogenetic analysis. RESULTS: A total of 121 different samples were HMPV laboratory-confirmed out of 6658 specimens received...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604066/simultaneous-identification-of-haemophilus-influenzae-and-haemophilus-haemolyticus-using-real-time-pcr
#8
Erin P Price, Tegan M Harris, Jessie Spargo, Elizabeth Nosworthy, Jemima Beissbarth, Anne B Chang, Heidi C Smith-Vaughan, Derek S Sarovich
AIM: To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. MATERIALS & METHODS: A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed. RESULTS & DISCUSSION: PCR screening across 143 isolates demonstrated 100% specificity for hypD and siaT...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28604063/vaginal-self-sampling-as-a-diagnosis-tool-in-low-income-countries-and-potential-applications-for-exploring-the-infectious-causes-of-miscarriage
#9
Jean-Christophe Lagier, Nafissatou Diagne, Florence Fenollar, Catherine Tamalet, Cheikh Sokhna, Didier Raoult
Gynecological health is a challenge in low-income countries. Personal opposition to perineal examination has been overcome by the use of vaginal self-sampling. Here, we review the use of this procedure notably in low-income countries and the main infectious causes of miscarriage. Vaginal self-sampling was mainly used for human papillomavirus detection but also to detect microorganisms causing sexually transmitted infections or bacterial vaginosis. 58 studies have been performed in low-resource countries, mainly studies performed to detect human papillomavirus in urban and peri-urban areas and demonstrating excellent acceptability...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28594235/live-attenuated-auxotrophic-mutant-of-salmonella-typhimurium-expressing-immunogenic-ha1-protein-enhances-immunity-and-protective-efficacy-against-h1n1-influenza-virus-infection
#10
Nitin Machindra Kamble, Kim Je Hyoung, John Hwa Lee
AIM: To evaluate the efficacy of attenuated Salmonella typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264...
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28593804/the-search-for-new-sporicidal-agents-for-medical-use-where-are-we
#11
Ewelina Piktel, Katarzyna Pogoda, Paul B Savage, Robert Bucki
No abstract text is available yet for this article.
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28593799/what-can-evolutionary-rescue-tell-us-about-the-emergence-of-new-resistant-bacteria
#12
Matti Jalasvuori, Reetta Penttinen
No abstract text is available yet for this article.
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28593794/cthtra-the-lynchpin-of-the-chlamydial-surface-and-a-promising-therapeutic-target
#13
James W Marsh, Vanissa A Ong, William B Lott, Peter Timms, Joel DA Tyndall, Wilhelmina M Huston
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection worldwide and the leading cause of preventable blindness. Reports have emerged of treatment failure, suggesting a need to develop new antibiotics to battle Chlamydia infection. One possible candidate for a new treatment is the protease inhibitor JO146, which is an effective anti-Chlamydia agent that targets the CtHtrA protein. CtHtrA is a lynchpin on the chlamydial cell surface due to its essential and multifunctional roles in the bacteria's stress response, replicative phase of development, virulence and outer-membrane protein assembly...
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28555497/ldtmav2-a-nonclassical-transpeptidase-and-susceptibility-of-mycobacterium-avium-to-carbapenems
#14
Rohini Mattoo, Evan P Lloyd, Amit Kaushik, Pankaj Kumar, Julie L Brunelle, Craig A Townsend, Gyanu Lamichhane
AIM: Mycobacterium avium infections, especially in immune-compromised individuals, present a significant challenge as therapeutic options are limited. In this study, we investigated if M. avium genome encodes nonclassical transpeptidases and if newer carbapenems are effective against this mycobacteria. MATERIALS & METHODS: Biochemical and microbiological approaches were used to identify and characterize a nonclassical transpeptidase, namely L,D-transpeptidase, in M...
May 30, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28541792/why-is-scientific-research-on-data-poor-microorganisms-being-ignored
#15
John P Hays
No abstract text is available yet for this article.
May 25, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28541127/screening-of-plants-with-antimicrobial-activity-against-enterobacteria-pseudomonas-spp-and-staphylococcus-spp
#16
Camila Miorelli Girondi, Analu Barros de Oliveira, Jackeline Alves Prado, Cristiane Yumi Koga-Ito, Aline Chiodi Borges, Alberto Carlos Botazzo Delbem, Daniel Freitas Alves Pereira, Marcos José Salvador, Fernanda Lourenção Brighenti
AIM: This study screened plants for antibacterial properties against bacteria of medical importance. MATERIALS & METHODS: 60 extracts were obtained from the leaves of ten plants (Jatropha weddelliana, Attalea phalerata, Buchenavia tomentosa, Croton doctoris, Mouriri elliptica, Mascagnia benthamiana, Senna aculeata, Unonopis guatterioides, Allagoptera leucocalyx and Bactris glaucescens) using different extraction methods: A) Ethanol 70°C/72 h; B) Water/5 min/100°C; C) Water/1 h/55°C; D) Water/72 h; E) Hexane/72 h and F) Ethanol 99°C/72 h...
May 25, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28541094/mobile-phones-create-new-opportunities-for-microbiology-research-and-clinical-applications
#17
Hatice Ceylan Koydemir, Aydogan Ozcan
No abstract text is available yet for this article.
May 25, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28541093/vascular-graft-infection-a-new-model-for-treatment-management
#18
Stefanie Deinhardt-Emmer, Verena Hoerr, Bettina Löffler
No abstract text is available yet for this article.
May 25, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28540747/in-vitro-susceptibility-and-resistance-phenotypes-in-contemporary-enterobacter-isolates-in-a-university-hospital-in-crete-greece
#19
Sofia Maraki, Konstantinos Z Vardakas, George Samonis, Dimitrios Perdikis, Viktoria Eirini Mavromanolaki, Diamantis P Kofteridis, Matthew E Falagas
AIM: To study the evolution in the susceptibility of Enterobacter spp. in Crete, Greece from 2010 to 2015. METHODS: Non-duplicate isolates were studied using automated systems. Phenotypic confirmatory tests were applied. RESULTS: A total of 939 Enterobacter isolates were included. Colistin was the most active antibiotic (97.9%) followed by imipenem (96.1%), gentamicin (95.7%), tigecycline (91.8%), cefepime (89.4%), chloramphenicol (85.8%), fosfomycin (85...
May 25, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28540732/mouse-model-of-colonization-of-the-digestive-tract-with-acinetobacter-baumannii-and-subsequent-pneumonia
#20
Noémie Coron, Silvie Pavlickova, Angélique Godefroy, Hélène Pailhoriès, Marie Kempf, Viviane Cassisa, Laurent Marsollier, Estelle Marion, Marie-Laure Joly-Guillou, Matthieu Eveillard
AIM: Implementing a mouse model of Acinetobacter baumannii (AB) digestive colonization and studying the propensity of an intestinal reservoir of AB to be at the origin of pneumonia. MATERIALS & METHODS: After a disruption of the digestive flora by piperacillin-tazobactam, two multidrug-resistant AB strains were intranasally inoculated to two cohorts of ten mice daily. For each strain, five mice were rendered transiently neutropenic. RESULTS & CONCLUSION: One strain persisted several weeks in the digestive tract, even after stopping piperacillin-tazobactam injections, leading to the hypothesis that some AB strains can authentically colonize the gut...
May 25, 2017: Future Microbiology
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