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Future Microbiology

Dan Zhang, Yijie Dong, Qilin Yu, Zhang Kai, Meng Zhang, Chang Jia, Chenpeng Xiao, Bing Zhang, Biao Zhang, Mingchun Li
AIM: Glutaredoxin is a conserved oxidoreductase in eukaryotes and prokaryotes. This study aimed to determine the role of Grx3 in cell survival, iron homeostasis and the oxidative stress response in Candida albicans. MATERIALS & METHODS: A grx3Δ/Δ mutant was obtained using PCR-mediated homologs recombination. The function of Grx3 was investigated by a series of biochemical methods. RESULTS: Deletion of GRX3 impaired growth and cell cycle, disturbance of iron homeostasis and activated the oxidative stress response...
October 17, 2017: Future Microbiology
Alok Kumar Singh, Pragya Yadav, Pratiksha Karaulia, Vinay Kumar Singh, Pushpa Gupta, Santosh Kumar Puttrevu, Sanjay Chauhan, Rabi S Bhatta, Narender Tadigoppula, Umesh D Gupta, Sidharth Chopra, Arunava Dasgupta
AIM: Our objective was to identify a more potent curcumin derivative with specific activity against Mycobacterium tuberculosis. MATERIALS & METHODS:  A total of 21 curcumin derivatives were synthesized and detailed bio-evaluation was carried out including determination of static/cidality, synergy with front-line antituberculosis drugs and determination of efficacy in the murine model of M. tuberculosis infection. RESULTS: We identified CPMD-6d dihydrochloride exhibiting concentration-dependent bactericidal activity against M...
October 16, 2017: Future Microbiology
Joanna Szaleniec, Andrzej Górski, Maciej Szaleniec, Ryszard Międzybrodzki, Beata Weber-Dąbrowska, Paweł Stręk, Jacek Składzień
Chronic rhinosinusitis (CRS) affects 5-15% of the global population. In some patients, the infectious exacerbations of the disease are recalcitrant to medical treatment and surgery. These cases are probably associated with the presence of bacterial biofilms. Bacteriophage (phage) therapy seems to be a promising antibiofilm strategy. The efficacy of phage therapy in sinonasal infections has been demonstrated both in vitro and in animal models. In the past, phage preparations were also administered to humans with CRS with favorable outcomes and no significant side effects...
October 13, 2017: Future Microbiology
Marta Esgalhado, Julie A Kemp, Nagila Rt Damasceno, Denis Fouque, Denise Mafra
Under physiologic conditions, the human gut microbiota performs several activities essential to the body health. In contrast, their imbalances exacerbate some actions which can promote a cascade of metabolic abnormalities, and vice versa. Numerous diseases, including chronic kidney disease, are associated with gut microbiota imbalance, and among several strategies to re-establish gut symbiosis, prebiotics seem to represent an effective nonpharmacological approach. Prebiotics fermentation by gut microbiota produce short-chain fatty acids, which improve the gut barrier integrity and function, and modulate the glucose and lipid metabolism as well as the inflammatory response and immune system...
October 13, 2017: Future Microbiology
Yachao Zhao, Lijun Wang, Zhiyi Zhang, Jiao Feng, Huaixing Kang, Liqun Fang, Xiaoyuan Jiang, Defu Zhang, Zhe Zhan, Dongsheng Zhou, Yigang Tong
AIM: To characterize a conjugative bla NDM-1-carrying plasmid pNDM-BTR from a clinical Escherichia coli isolate. MATERIALS & METHODS: The complete nucleotide sequence of pNDM-BTR was determined using next-generation sequencing technology. Comparative genomic analysis of bla NDM-carrying IncN1 plasmids, including pNDM-BTR, was performed, and the antimicrobial resistance phenotypes were determined. RESULTS: pNDM-BTR contained three accessory modules, namely IS26, a novel Tn3-family transposon Tn6360 and the dfrA14 region composed of In191, ecoRII-ecoRIImet and ΔIS1X2...
October 13, 2017: Future Microbiology
Vinod Shanmughapriya, Stephen Richard, Tamilmaran Nagarajan, M Hussain Munavar
AIM: The ssrA mutants were found to be more sensitive to mitomycin C (MMC) and our aim was to study this phenomenon in detail. MATERIALS & METHODS: Strains were constructed by P1 transduction. pssrA(+) plasmid was constructed by PCR-based cloning and transformation was done by CaCl2 method. Relative viability analyses were done to assess the extent of viability of strains in relevant conditions. Gram staining was used for microscopic analysis. RESULTS: ssrA mutants become sensitive specifically to MMC, that too in a strain-specific manner...
October 13, 2017: Future Microbiology
Ranjani Somayaji, Christopher Naugler, Maggie Guo, Deirdre L Church
AIM: Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection in developed nations and is an important public health concern. We aimed to assess the factors associated with testing and positivity of C. trachomatis in a large population. METHODS: A retrospective study of a large Canadian health region was undertaken using 2011 census and laboratory data. Demographic and socioeconomic data from the national household survey were linked to microbiologic data for C...
October 12, 2017: Future Microbiology
Marc Xipell, Marta Bodro, Francesc Marco, José A Martínez, Alex Soriano
Ceftolozane/tazobactam is a novel fifth-generation cephalosporin β-lactamase combination with activity against extended-spectrum β-lactamases-producing enterobacteriaceae, and multidrug resistant Pseudomonas aeruginosa. However, clinical experience in real cases caused by these microorganisms is scarce. In this study, we describe three patients with severe infections caused by multidrug resistant and extensively drug-resistant (XDR) P. aeruginosa that were successfully treated with ceftolozane/tazobactam.
October 5, 2017: Future Microbiology
Mohsin Khurshid, Muhammad Hidayat Rasool, Usman Ali Ashfaq, Bilal Aslam, Muhammad Waseem
AIM: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates. MATERIALS & METHODS: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A...
October 5, 2017: Future Microbiology
Gianmarco Gasperini, Vanessa Arato, Mariagrazia Pizza, Beatrice Aricò, Rosanna Leuzzi
AIM: Bordetella pertussis has been shown to release outer membrane vesicles (OMV) both in vitro and in vivo but little is known about their biological role during the initial phases of B. pertussis infection of the airways. RESULTS: We have demonstrated that OMV are released by B. pertussis in a human ciliated-airway cell model and purified vesicles can interact with host cells. Binding and uptake are strictly Bvg-regulated and OMV-associated. Pertussis toxin contributes to host-cell intoxication...
October 5, 2017: Future Microbiology
Birgitte H Kallipolitis
No abstract text is available yet for this article.
October 4, 2017: Future Microbiology
Sabrina De Carolis, Alice Pellegrini, Donatella Santini, Claudio Ceccarelli, Antonio De Leo, Federica Alessandrini, Chiara Arienti, Sara Pignatta, Anna Tesei, Vilma Mantovani, Claudio Zamagni, Mario Taffurelli, Pasquale Sansone, Massimiliano Bonafé, Monica Cricca
AIM: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. MATERIALS & METHODS: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. RESULTS: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas...
October 4, 2017: Future Microbiology
Juliana Galletti, Flávia K Tobaldini-Valerio, Sónia Silva, Érika Seki Kioshima, Larissa Trierveiler-Pereira, Marcos Bruschi, Melyssa Negri, Terezinha Inez Estivalet Svidzinski
AIM: The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form biofilms and the antibiofilm effect of propolis extract on these biofilms. MATERIALS & METHODS: The biofilms and antibiofilm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy. RESULTS: Propolis extract demonstrated significant antibiofilm efficiency on Fusarium spp...
October 4, 2017: Future Microbiology
Tânia P Salci, Melyssa Negri, Ana Karina R Abadio, P Bonfim-Mendonça, Isis Capoci, Silvana M Caparroz-Assef, Lucélia Donati, Maria Sueli S Felipe, Erika S Kioshima, Terezinha Ie Svidzinski
AIM: To investigate the antifungal activity of MOL3, a small molecule that was selected by virtual screening, against Candida spp. MATERIALS & METHODS: The antifungal activity of MOL3 was evaluated using standard strains and clinical isolates. Activity was evaluated in both in vitro tests and animal models. RESULTS: The minimum fungicidal concentration of MOL3 against Candida spp. ranged from 16 to 128 mg/l. MOL3 at the sub-minimum fungicidal concentration inhibited hyphal elongation...
October 4, 2017: Future Microbiology
Feng-Jun Li, Cynthia Y He
No abstract text is available yet for this article.
October 3, 2017: Future Microbiology
Jean-Philippe Lavigne, Albert Sotto
No abstract text is available yet for this article.
October 3, 2017: Future Microbiology
Todd Z DeSantis, Manasi S Shah, Julia L Cope, Emily B Hollister
No abstract text is available yet for this article.
October 3, 2017: Future Microbiology
Dinesh Kumar Dahiya
No abstract text is available yet for this article.
September 27, 2017: Future Microbiology
Sudong Zhang, Yi He, Erlie Jiang, Jialin Wei, Donglin Yang, Rongli Zhang, Weihua Zhai, Guixin Zhang, Zhao Wang, Lining Zhang, Li Liu, Mingzhe Han, Sizhou Feng
AIM: Invasive fungal disease (IFD) is a significant cause of morbidity and mortality in hematopoietic stem cell transplantation (HSCT) patients. This study reports real-world efficacy and safety of posaconazole (POS) in HSCT recipients with IFD. PATIENTS & METHODS: We performed a retrospective study to investigate the efficacy and safety of POS oral suspension in 45 HSCT patients with IFD from December 2013 to December 2016. RESULTS: The success rate at 12 weeks after POS treatment was 57...
September 7, 2017: Future Microbiology
Kai Zhang, Chang Jia, Qilin Yu, Chenpeng Xiao, Yijie Dong, Meng Zhang, Dan Zhang, Qiang Zhao, Biao Zhang, Mingchun Li
AIM: V-ATPase is a conservative multi-subunit enzyme in eukaryotes and modulates several cellular responses. This study aimed to illustrate the roles of Vma5 in vacuolar function, oxidative stress response, calcium homeostasis, autophagy and virulence. MATERIALS & METHODS: The vma5Δ/Δ mutant was obtained using PCR-mediated homologous recombination. The functions of Vma5 were investigated by a series of biochemical and systemic infection methods. RESULTS: Disruption of VMA5 led to growth inhibition, vacuolar dysfunction, disturbance of calcium homeostasis and inhibition of calcium-related oxidative stress response...
September 7, 2017: Future Microbiology
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