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Future Microbiology

Vitalij Novickij, Jurgita Švedienė, Algimantas Paškevičius, Svetlana Markovskaja, Irutė Girkontaitė, Auksė Zinkevičienė, Eglė Lastauskienė, Jurij Novickij
AIM: Determine the influence of pH on the inactivation efficiency of C. albicans in pulsed electric fields (PEF) and evaluate the possibilities for sensitization of a drug-resistant strain to antifungal drugs. MATERIALS & METHODS: The effects of PEF (2.5-25 kVcm-1) with fluconazole, terbinafine and naftifine were analyzed at a pH range of 3.0-9.0. Membrane permeabilization was determined by flow cytometry and propidium iodide. RESULTS: PEF induced higher inactivation of C...
December 11, 2017: Future Microbiology
Ying Hua, Jingwei Ju, Xiangyu Wang, Bao Zhang, Wei Zhao, Qiwei Zhang, Yingzhu Feng, Wenbin Ma, Chengsong Wan
AIM: To screen host proteins that interact with enterohemorrhagic Escherichia coli O157:H7 EspF. MATERIALS & METHODS: Flow cytometry and high-throughput sequencing were used to screen interacting proteins. Molecular function, biological processes and Kyoto Encyclopedia of Genes and Genomes pathways were studied using the DAVID online tool. Glutathione S-transferase pull down and dot blotting were used to verify the interactions. RESULTS: 293 host proteins were identified to associate with EspF...
December 11, 2017: Future Microbiology
Virginia Carvalhais, Begoña Pérez-Cabezas, Cátia Oliveira, Rui Vitorino, Manuel Vilanova, Nuno Cerca
AIM: The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates. MATERIALS & METHODS: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumeration of viable and cultivable cells, and confocal microscopy...
December 11, 2017: Future Microbiology
J Andrés Valderrama, Victor Nizet
Group A Streptococcus (GAS) is a leading human bacterial pathogen with diverse clinical manifestations. Macrophages constitute a critical first line of host defense against GAS infection, using numerous surface and intracellular receptors such as Toll-like receptors and inflammasomes for pathogen recognition and activation of inflammatory signaling pathways. Depending on the intensity of the GAS infection, activation of these signaling cascades may provide a beneficial early alarm for effective immune clearance, or conversely, may cause hyperinflammation and tissue injury during severe invasive infection...
December 11, 2017: Future Microbiology
Sima Tokajian, Rima Moghnieh, Tamara Salloum, Harout Arabaghian, Sahar Alousi, Jennifer Moussa, Edmond Abboud, Souad Youssef, Rola Husni
AIM: To evaluate the effects of population influx of refugees on the prevalence of extended-spectrum β-lactamase-producing Escherichia coli in wastewater networks in Lebanon. MATERIALS & METHODS: Pulsed-field gel electrophoresis, multilocus sequence typing and antibiotic resistance genes typing were performed. RESULTS: 53.1% of isolates recovered from Al-Qaa refugee camp were positive for the tested resistant determinants compared with 49...
December 11, 2017: Future Microbiology
Muhammad Usman Qamar, Sidrah Saleem, Mark Alexander Toleman, Muhammad Saqalein, Muhammad Waseem, Muhammad Atif Nisar, Mohsin Khurshid, Zeeshan Taj, Shah Jahan
AIM: To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo. MATERIALS & METHODS: Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system...
December 11, 2017: Future Microbiology
Edward Pk Parker, Sasirekha Ramani, Benjamin A Lopman, James A Church, Miren Iturriza-Gómara, Andrew J Prendergast, Nicholas C Grassly
Oral vaccines are less immunogenic when given to infants in low-income compared with high-income countries, limiting their potential public health impact. Here, we review factors that might contribute to this phenomenon, including transplacental antibodies, breastfeeding, histo blood group antigens, enteric pathogens, malnutrition, microbiota dysbiosis and environmental enteropathy. We highlight several clear risk factors for vaccine failure, such as the inhibitory effect of enteroviruses on oral poliovirus vaccine...
December 8, 2017: Future Microbiology
Elena Perrin, Valentina Maggini, Isabel Maida, Eugenia Gallo, Katia Lombardo, Maria Pia Madarena, Silvia Buroni, Viola Camilla Scoffone, Fabio Firenzuoli, Alessio Mengoni, Renato Fani
AIM: To investigate the activity and mechanisms of action of six essential oils (EOs) against Burkholderia cepacia complex, opportunistic human pathogens highly resistant to antibiotics. MATERIALS & METHODS: Minimal inhibitory concentration of EOs alone, plus antibiotics or efflux pump inhibitors was determined. RESULTS: Origanum vulgare, Thymus vulgaris and Eugenia caryophyllata EOs resulted to be more active than the other EOs. EOs did not enhance antibiotic activity against the model strain B...
December 4, 2017: Future Microbiology
Jobje Haayman, Ellen E Stobberingh
No abstract text is available yet for this article.
December 4, 2017: Future Microbiology
Garry Laverty
No abstract text is available yet for this article.
December 4, 2017: Future Microbiology
Anna M Grudniak, Barbara Klecha, Krystyna I Wolska
AIM: Pseudomonas aeruginosa is one of the most clinically important opportunistic pathogen in humans. The aim of the project was to study effects of HtpG on the selected virulence factors responsible for pathogenesis and biofilm formation of P. aeruginosa. METHODOLOGY: By characterizing a htpG null mutant of P. aeruginosa, we have identified the role of HtpG in the production of selected factors. RESULTS: We showed that ΔhtpG mutant affects many physiological processes containing: decreased activity of the LasA protease, reduction of biofilm formation, decreased motility, and diminished amount of rhamnolipids and pyoverdine/pyocyanin...
December 4, 2017: Future Microbiology
Glauber R de S Araújo, Wanderley de Souza, Susana Frases
Invasive fungal infections are a growing threat to immunocompromised patients, highlighting the importance of monitoring fungal pathogens. Global warming (including climatic oscillations) may select for environmental species that have acquired thermotolerance, a key step toward pathogenesis to humans. Also, important virulence factors have developed in environmental fungi, because they are essential for yeast survival in the environment. Thus, fungi traditionally regarded as nonpathogenic to humans have virulence factors similar to those of their pathogenic relatives...
November 23, 2017: Future Microbiology
Vânia Gaio, Cláudia Afonso Lima, Fernando Oliveira, Ângela França, Nuno Cerca
AIM: To evaluate the antimicrobial activity of carvacrol against stationary-phase planktonic and biofilm cells of coagulase-negative staphylococci and comparison to traditional antistaphylococcal antibiotics. MATERIALS & METHODS: The antimicrobial effect of carvacrol and antibiotics against planktonic and biofilm cells were assessed through quantification of the number of culturable and/or viable cells. Confocal laser scanning microscopy was used to evaluate the effect of carvacrol on the biofilm structure...
November 23, 2017: Future Microbiology
Lizhi Ma, Zhe Yin, Defu Zhang, Zhe Zhan, Qian Wang, Xiongbo Duan, Huixia Gao, Quanhui Liang, Yuzong Zhao, Jiao Feng, Yachao Zhao, Yigang Tong, Erhei Dai, Dongsheng Zhou
AIM: This study dealt with genomic characterization of type 1 IncC resistance plasmids, capable of spreading across taxonomic borders, from China. MATERIALS & METHODS: p112298-tetA was sequenced and compared with type 1 IncC reference plasmid pR148 and two available sequenced type 1 IncC plasmids pHS36-NDM and pVAS3-1 from China. RESULTS: These plasmids contained one or more exogenous resistance islands, which included the ARI-A islands, the ARI-B islands, the ISEcp1-blaCMY units and the bla KPC-2 region and were inserted at various sites in the IncC backbone and thus represented three distinct lineages...
November 15, 2017: Future Microbiology
Rafael Melo Santos de Serpa Brandão, Angélica Rosa Faria, Hélida Monteiro de Andrade, Liline Maria Soares Martins, Adalberto Socorro da Silva, Semiramis Jamil Hadad do Monte
AIM: To produce and test recombinant multiepitope proteins as an alternative assay for the serological diagnosis of cryptococcosis. MATERIALS & METHODS: Previously, synthetic peptides were used to detect anti-Cryptococcus antibodies, and in silico analyses showed that the union of peptides would improve the results. Here, the coding sequences of these peptides were assembled into synthetic genes. Four genes have been cloned and expressed in Escherichia coli, producing recombinant multiepitope proteins: proteins A, B, C and D...
November 10, 2017: Future Microbiology
De-Dong Li, Beth Burgwyn Fuchs, Yan Wang, Xiao-Wen Huang, Dan-Dan Hu, Yan Sun, Dong Chai, Yuan-Ying Jiang, Eleftherios Mylonakis
AIM: To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. albicans. MATERIALS & METHODS: C. albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism. RESULTS: The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models...
November 7, 2017: Future Microbiology
Eliane M da Silva, Brenda Kischkel, Cristiane S Shinobu-Mesquita, Patrícia S Bonfim-Mendonça, Elaine Sb Mansano, Marcos A da Silva, Jéssica F Barbosa, Adriana Fiorini, Luzmarina Hernandes, Marcia C Furlaneto, Terezinha Ie Svidzinski
AIM: To evaluate if radiation used in radiotherapy can cause changes in the virulence potential of Candida tropicalis ATCC 750. MATERIALS & METHODS: C. tropicalis was exposed in vitro to identical dose and scheme of irradiation would be used in patients with head and neck cancer. Some virulence parameters were analyzed before and after irradiation. RESULTS: Colony morphologies were irreversibly affected by irradiation. Increase in growth rate, filamentation, adhesion on cell lines and phagocytosis process were also observed...
November 7, 2017: Future Microbiology
Yao-Ting Huang, Jan-Fang Cheng, Yi-Ting Liu, Yan-Chiao Mao, Meng-San Wu, Po-Yu Liu
AIM: Serratia marcescens wound infection after snakebite is often associated with aggressive presentations. However, the virulence determinants remain understudied. MATERIALS & METHODS: Whole-genome sequencing was performed on S. marcescens VGH107, an isolate from wound infection secondary to Trimeresurus mucrosquamatus bite. Comparative genomics approach coupled with multivirulent-locus sequencing typing was applied to systematically predict potential virulence factors...
November 6, 2017: Future Microbiology
Saikat Paul, Pankaj Singh, Shivaprakash M Rudramurthy, Arunaloke Chakrabarti, Anup K Ghosh
AIM: To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion of existing Bruker Biotyper database for mold identification. MATERIALS & METHODS: Four different sample preparation methods (protocol A, B, C and D) were evaluated. RESULTS: On analyzing each protein extraction method, reliable identification and best log scores were achieved through protocol D. The same protocol was used to identify 153 clinical isolates...
October 27, 2017: Future Microbiology
Carla López-Causapé, Antonio Oliver
No abstract text is available yet for this article.
October 25, 2017: Future Microbiology
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