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Regenerative Medicine

Mukta S Sane, Neha Misra, Omid Mohammad Mousa, Steve Czop, Huiyuan Tang, Larry T Khoo, Christopher D Jones, Soumyajit Banerjee Mustafi
AIM: Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp). MATERIALS & METHODS: Luminex multiplex assay and standard cell biology methods were employed. RESULTS: Study with allografts from 33 donors identified 44 quantifiable cytokines in the UCBp derived conditioned media (CM). The UCBp-CM elevated proliferation and migration rates of mesenchymal stem cells (MSCs) and bone marrow stromal cells...
October 22, 2018: Regenerative Medicine
Yu-Jing Liao, Pin-Chi Tang, Chih-Hsun Lin, Lih-Ren Chen, Jenn-Rong Yang
AIM: We created rat models of osteoporosis and verified a novel idea to recover bone mass via local cell transplantation. MATERIALS & METHODS: The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells were transplanted into the medullary cavity of the left femurs. RESULTS: The piPSC-derived osteoblast-like cells exerted therapeutic potential on prednisolone treatment group, which confirmed by improvements in trabecular bone volume (15...
September 26, 2018: Regenerative Medicine
Lin Gong, Xue-Gang Xu, Yuan-Hong Li
Wound-induced hair follicle neogenesis (WIHN) is a regenerative phenomenon that occurs widely in the skin of adult mammalians. A fully functional follicle can regenerate in the center of a full-thickness wound with a large enough size. The cellular origin of this process is similar to embryonic process. Many growth and development-related pathways are involved in WIHN. Studying WIHN can deeply explore the mechanism of biological growth, development and regeneration, and can identify new treatments for hair-related disorders...
September 26, 2018: Regenerative Medicine
Isabel Andia, Nicola Maffulli
The positive extensive clinical experience with platelet-rich plasma (PRP) in different medical areas has prompted researchers to explore clinical opportunities for optimized PRP therapies. PRP is safe but we have to make it more effective. The growing diversity of formulations and presentations enrich the field of PRP research and offer hope to refine clinical indications. Moving toward targeting the right disease phenotypes with the right PRP formulation or combination product (PRP + cell products) can offer opportunities to change treatment options in osteoarthritis and nonhealing wounds...
September 24, 2018: Regenerative Medicine
Nadia Naseer, Saliha Bashir, Noreen Latief, Farzana Latif, Shaheen N Khan, Sheikh Riazuddin
AIM: The aim of the present study is to use human amniotic membrane (HAM) for in vitro chondrogenesis of placenta-derived mesenchymal stem cells (MSCs) and umbilical cord-derived MSCs. MATERIALS & METHODS: MSCs from the placenta and umbilical cord were isolated, characterized by immunophenotyping and after analyzing their rate of proliferation, cytotoxicity and viability, chondrogenesis was performed on plastic adherent surface and on HAM. RESULTS: Successfully isolated and characterized placenta-derived MSCs and umbilical cord-derived MSCs revealed positive expression of MSCs markers CD90, CD73, CD105 and CD49d, while they were negative for CD45...
October 2018: Regenerative Medicine
Timothy R Olsen, Jon A Rowley
RoosterBio, Inc. (MD, USA) is a privately held stem cell tools and technology company focused on accelerating the development of a sustainable regenerative medicine industry, one customer at a time. RoosterBio's products are high-volume and well-characterized adult human mesenchymal stem/stromal cells (hMSCs) paired with highly engineered media systems. RoosterBio has aimed to simplify and standardize how stem cells are purchased, expanded and used in the development of regenerative medicine products. To this end, RoosterBio supplies off-the-shelf cGMP hMSC working cell banks with bioprocess media that mimic the format and formulation of the research grade counterparts, radically simplifying and shortening product development and clinical translation...
October 2018: Regenerative Medicine
Karina P Reis, Laura E Sperling, Cristian Teixeira, Ágata Paim, Bruno Alcântara, Gema Vizcay-Barrena, Roland A Fleck, Patricia Pranke
AIM: Scaffolds are a promising approach for spinal cord injury (SCI) treatment. FGF-2 is involved in tissue repair but is easily degradable and presents collateral effects in systemic administration. In order to address the stability issue and avoid the systemic effects, FGF-2 was encapsulated into core-shell microfibers by coaxial electrospinning and its in vitro and in vivo potential were studied. Materials & methods: The fibers were characterized by physicochemical and biological parameters...
October 2018: Regenerative Medicine
Paul S Knoepfler
The stem cell and regenerative medicine arena has become increasingly complicated in recent years with thousands of people involved. There are as many as a dozen or more main groups of stakeholders, who together may be viewed as one ecosystem that is now rapidly evolving. The nature of the ecosystem and its evolution have major implications for not just those within it, but also for medicine and society at large. Here, I describe this ecosystem and its evolution, as well as the negative impacts within the ecosystem of a constellation of hundreds of unproven for-profit clinics and related businesses...
October 2018: Regenerative Medicine
Letícia Queiroz da Silva, Silmara Aparecida de Lima Montalvão, Amauri da Silva Justo-Junior, José Luiz Rosenberis Cunha Júnior, Stephany Cares Huber, Carolina Caliári Oliveira, Joyce Maria Annichino-Bizzacchi
AIMS: To compare levels and activity of the growth factors between fresh and lyophilized platelet-rich plasma (PRP). METHODS: Analysis of platelet concentration using fibroblast and human umbilical vein endothelial cell cultures were compared between fresh and lyophilized PRP obtained from peripheral blood. RESULTS: After lyophilization, 54% of platelets were intact whereas the fresh showed no aggregation with agonists (levels under 20%). The concentration of growth factors (VEGF, EGF, TGF-β and PDGF) in both products were similar...
October 2018: Regenerative Medicine
Leila Zare, Hossein Baharvand, Mohammad Javan
AIM: It would be clinically ideal to target astrocytes in vivo for conversion into oligodendrocyte lineage cells to reduce astrogliosis and generate new myelinating cells. MATERIALS & METHODS: Here, we prepared a GFP-labeled human astrocyte cell line, treated with epigenetic modifiers trichostatin A or 5-azacytidine and transplanted them into cuprizone-induced demyelinated mice brains. The fate of the transplanted astrocytes was studied at days 7, 14 and 28 post-transplantation...
October 2018: Regenerative Medicine
Jacob Spinnen, Jochen Ringe, Michael Sittinger
Due to its chemoattraction potential on mesenchymal stromal cells of the CCL25/CCR9 axis, local application of CCL25 to severely damaged tissues may be a promising approach for regenerative therapies. Analysis of the given data revealed that CCL25/CCR9 signaling has a crucial role in regulation of an adult immune homeostasis. CCR9 expression variations resulted in dysfunctional immune response in colitis, rheumatoid arthritis and endometriosis. Regarding oncology, different neoplastic tissues exploit CCL25-dependent CCR9 signaling for either local proliferation or migration processes...
October 2018: Regenerative Medicine
Stephen Sullivan, Glyn N Stacey, Chihiro Akazawa, Naoki Aoyama, Ricardo Baptista, Patrick Bedford, Annelise Bennaceur Griscelli, Amit Chandra, Ngaire Elwood, Mathilde Girard, Shin Kawamata, Tadaaki Hanatani, Theodoros Latsis, Stephen Lin, Tenneille E Ludwig, Tamara Malygina, Amanda Mack, Joanne C Mountford, Scott Noggle, Lygia V Pereira, Jack Price, Michael Sheldon, Alok Srivastava, Harald Stachelscheid, Shaji R Velayudhan, Natalie J Ward, Marc L Turner, Jacqueline Barry, Jihwan Song
Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation of cellular therapeutics requires demonstration of comparability of lines derived from different individuals and in different facilities. This requires agreement on the critical quality attributes of such lines and the assays that should be used. Working from established recommendations and guidance from the International Stem Cell Banking Initiative for human embryonic stem cell banking, and concentrating on those issues more relevant to iPSCs, a series of consensus workshops has made initial recommendations on the minimum dataset required to consider an iPSC line of clinical grade, which are outlined in this report...
October 2018: Regenerative Medicine
D Adam Young, Kirk C McGilvray, Nicole Ehrhart, Thomas W Gilbert
AIM: Biologically derived surgical graft materials come from a variety of sources with varying mechanical properties. This study aimed to evaluate the host response and mechanical performance of two extracellular matrix devices in a large animal preclinical model. MATERIALS & METHODS: Bilateral defects were created in the fascia lata of sheep and repaired with either an acellular dermal matrix (ADM) or urinary bladder matrix (UBM). After 1 or 3 months, the repair site was explanted for histological and mechanical analysis...
October 2018: Regenerative Medicine
Leigh Turner
AIM: This study examines marketing claims of Canadian businesses engaged in direct-to-consumer advertising of putative stem cell treatments. METHODS: Internet searches were used to locate Canadian businesses selling stem cell interventions. Company websites were subjected to detailed analysis. RESULTS: In total, 30 Canadian businesses sell stem cell interventions provided at 43 clinics. Autologous stem cells are the most common types of products promoted by such businesses...
September 2018: Regenerative Medicine
Yunqing Kang, Jia Chang
Vascularization is essential for tissue regeneration. Despite extensive efforts in the past decades, sufficient and rapid vascularization remains a major challenge in tissue engineering. Many studies have shown that the addition of channels in a porous scaffold can provide the ability to promote cell growth and rapid vascularization, thus leading to better outcomes in new tissue formation. Large size scaffolds lack perfusable channel networks and negatively impair the survival of transplanted cells and tissue function development, leading to necrotic core formation and the failure of functional tissue formation...
September 2018: Regenerative Medicine
Luc Douay
No abstract text is available yet for this article.
September 2018: Regenerative Medicine
Mukta S Sane, Neha Misra, Nathan M Quintanar, Christopher D Jones, Soumyajit Banerjee Mustafi
AIM: Placental allografts used for tissue regeneration differ in membrane compositions and processing techniques. A uniquely folded dehydrated binate amniotic membrane (DBAM) was biochemically characterized to evaluate its potential role in wound healing. METHODS: Histology, Luminex-based immunoassay and standard in vitro cell biology techniques were employed. RESULTS: Histological staining confirmed that the DBAM was chorion free with epithelial cell layer of the respective amnion membranes facing outward...
September 2018: Regenerative Medicine
Seydina B Touré, Erika Kleiderman, Bartha M Knoppers
Stem cells may not systematically obey traditional Phase I-IV clinical translation models. In response, various actors have suggested that stem cell-based medical innovation models could catalyze translation instead. Accordingly, calls were made to adopt more permissive approaches to stem cell translation. Yet, the Phase I-IV paradigm remains the standard within the scientific community. This article seeks to advance the stalemated discussions by proposing an alternative model for consideration. In it, we argue that a stem cell-based learning health system may be able to reconcile these two models...
September 2018: Regenerative Medicine
Hiromi Yagi Mendoza, Tomomi Yokoyama, Tomoko Tanaka, Hisataka Ii, Ken Yaegaki
AIM: In this study, we aimed to establish the differentiation protocol of dental pulp stem cells (DPSCs) into pancreatic islets using a 3D structure. MATERIALS & METHODS: DPSCs were differentiated in a 3D culture system using a stepwise protocol. Expression of β-cell markers, glucose-stimulated insulin secretion, and PI3K/AKT and WNT pathways were compared between monolayer-cultured pancreatic cells and islets. RESULTS: Islet formation increased insulin and C-peptide production, and enhanced the expression of pancreatic markers...
July 20, 2018: Regenerative Medicine
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