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Regenerative Medicine

Anuradha Vaidya, Vaijayanti Kale, Mariyah Poonawala, Suprita Ghode
AIM: To examine the stromal cell-mediated effects of trans-resveratrol (TRV) on the fate of hematopoietic stem cells (HSCs). Materials & methods: Proliferation assay, cell cycle analysis, apoptosis assay, flow cytometry, western blot. RESULTS: Using KG1a, we show that TRV has a dose-dependent effect on the proliferation of hematopoietic cells. Its stimulatory effect was significantly enhanced when the cells were cocultured with stromal cells. Addition of TRV in the coculture of murine bone marrow-derived HSCs and stromal cells led to a significant increase in the pool of long-term HSCs...
May 10, 2018: Regenerative Medicine
D Adam Young, Nicolette Jackson, Catherine A Ronaghan, Collin Em Brathwaite, Thomas W Gilbert
AIM: Not all biologically derived materials elicit the same host response when used for reinforcement of ventral hernia repairs. This study aimed to evaluate the remodeling characteristics of the abdominal wall following reinforcement with urinary bladder matrix (UBM) in a large animal preclinical model of ventral hernia repair. MATERIALS & METHODS: Midline defects in 36 Yucatan minipigs were reinforced with UBM-derived surgical devices using a classic Rives-Stoppa-Wantz approach, and compared with primary repair controls...
May 4, 2018: Regenerative Medicine
Josephine K McEwan, Howard C Tribe, Neal Jacobs, Nicholas Hancock, Amir A Qureshi, Douglas G Dunlop, Richard Oc Oreffo
Bone is a highly specialized connective tissue and has a rare quality as one of the few tissues that can repair without a scar to regain pre-injury structure and function. Despite the excellent healing capacity of bone, tumor, infection, trauma and surgery can lead to significant bone loss requiring skeletal augmentation. Bone loss in the lower limb poses a complex clinical problem, requiring reconstructive techniques to restore form and function. In the past, amputation may have been the only option; however, there is now an array of reconstructive possibilities and cellular therapies available to salvage a limb...
June 2018: Regenerative Medicine
Elizabeth A Lucich, Juan L Rendon, Ian L Valerio
full-thickness skin defects remain a reconstructive challenge. Novel regenerative modalities can aid in addressing these defects. A literature review of currently available dermal and epidermal regenerates was performed. The mechanism and application for each skin substitute was analyzed to provide a guide for these modalities. Available epidermal substitutes include autografts and allografts and may be cultured or noncultured. Dermal regenerate templates exist in biologic and synthetic varieties that differ in the source animal and processing...
June 2018: Regenerative Medicine
Jeremy Snyder, Leigh Turner
AIM: To better understand how prospective customers interpret claims of businesses marketing unproven stem cell products that they are engaging in research activities. MATERIALS & METHODS: The authors examined 408 crowdfunding campaigns for unproven stem cell interventions for references to research activities. RESULTS: The authors identified three overarching themes: research as a signifier of scientific credibility; the experimental nature of stem cells as a rationale for noncoverage by insurers; and contributing to the advancement of science by engaging in research...
June 2018: Regenerative Medicine
Gisselle Escobar, Alejandro Escobar, Gabriel Ascui, Fabián I Tempio, María C Ortiz, Claudio A Pérez, Mercedes N López
AIM: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. MATERIALS & METHODS: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24 h stimulation with P-PRP or S-PRP. RESULTS: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker...
June 2018: Regenerative Medicine
Gloria Pelizzo, Maria Antonietta Avanzini, Antonia Icaro Cornaglia, Annalisa De Silvestri, Melissa Mantelli, Paola Travaglino, Stefania Croce, Piero Romano, Luigi Avolio, Giulio Iacob, Massimo Dominici, Valeria Calcaterra
AIM: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model. METHODS: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections...
June 2018: Regenerative Medicine
Khawaja H Haider
Nearly two decades of experimental and clinical research with bone marrow cells have paved the way for Phase III pivotal trials in larger groups of heart patients. Despite immense advancements, a multitude of factors are hampering the acceptance of bone marrow cell-based therapy for routine clinical use. These include uncertainties regarding purification and characterization of the cell preparation, delivery protocols, mechanistic understanding and study end points and their methods of assessment. Clinical data show mediocre outcomes in terms of sustained cardiac pump function...
June 2018: Regenerative Medicine
Richard Jp Smith, Adam J Reid
No abstract text is available yet for this article.
June 2018: Regenerative Medicine
M Terry Loghmani, Joseph A Roche
The 6th International Symposium on Regenerative Rehabilitation, hosted by the Alliance for Regenerative Rehabilitation Research and Training (AR3 T), included a preconference meeting of institutional representatives of the International Consortium of Regenerative Rehabilitation, keynote talks from distinguished scientists, platform and poster presentations from experts and trainees, panel discussions and postconference workshops. The following priorities were identified: increasing rigor in basic, preclinical and clinical studies, especially the use of better controls; developing better outcome measures for preclinical and clinical trials; focusing on developing more tissue-based interventions versus cell-based interventions; including regenerative rehabilitation in curricula of professional programs like occupational and physical therapy; and developing better instruments to quantify rehabilitative interventions...
April 3, 2018: Regenerative Medicine
Zhe Zhou, Hao Yan, Yidong Liu, Dongdong Xiao, Wei Li, Qiong Wang, Yang Zhao, Kang Sun, Ming Zhang, Mujun Lu
AIM: The study investigated the feasibility of seeding adipose-derived stem cells (ASCs) onto a poly(ϵ-caprolactone)/chitosan (PCL/CS) scaffold for bladder reconstruction using a rat model of bladder augmentation. MATERIALS & METHODS: In the experimental group, the autologous ASCs were seeded onto the PCL/CS scaffold for bladder augmentation. An unseeded scaffold was used for bladder augmentation as control group. The sham group was also set. RESULT: 8 weeks after implantation, more densely smooth muscles were detected in the experimental group with a larger bladder capacity and more intensive blood vessels...
April 2018: Regenerative Medicine
Triantafyllos Didangelos, George Koliakos, Kokkona Kouzi, George Arsos, Katerina Kotzampassi, Konstantinos Tziomalos, Dimitrios Karamanos, Apostolos I Hatzitolios
We describe the case of a Type I diabetic patient with a refractory foot ulcer that remained unhealed for 2 years despite conventional therapy. Autologous adipose-derived stromal vascular fraction suspended in autologous platelet-rich plasma was applied to the wound, which completely healed within 1 month. The wound remained closed with no complications for a 2-year follow-up. Reporting of this and similar cases may lead to larger clinical trials that will prove the efficacy of this therapy that may offer accelerated healing and lessen the financial burden of more expensive therapeutic modalities...
April 2018: Regenerative Medicine
Andreas West Vilsbøll, Jakob Munk Mouritsen, Line Park Jensen, Nikolaj Bødker, Annette Willemoes Holst, Cristian P Pennisi, Lars Ehlers
AIM: To perform an early cost-effectiveness analysis of in vitro expanded myoblasts (IVM) and minced myofibers versus midurethral slings (MUS) for surgical treatment of female stress urinary incontinence. METHODS: Cost-effectiveness and sensitivity analyses were performed using a decision tree comprising previously published data and expert opinions. RESULTS & CONCLUSION: In the base case scenario, MUS was the cost-effective strategy with a negative incremental cost-effectiveness ratio compared with IVM and a positive incremental cost-effectiveness ratio compared with minced myofibers...
April 2018: Regenerative Medicine
Adam Price-Evans
No abstract text is available yet for this article.
April 2018: Regenerative Medicine
Vitali Goriainov, Josephine K McEwan, Richard Oc Oreffo, Doug G Dunlop
Joint replacements have proved a medical success providing symptomatic relief and return to mobility in many patients with arthritis. However, multiple revision surgeries due to joint failure can result in complex revision scenarios with significant bone tissue loss, in an elderly population, which poses a significant clinical challenge. Computer-aided design-computer-assisted manufacturing (CAD-CAM) prototyped bespoke implants are currently being used as an alternative and innovative approach for joint restoration in salvage cases, while the incorporation of autologous skeletal stem cells to optimize regenerative capacity can enhance implant osseointegration...
April 2018: Regenerative Medicine
Kayvan Zainabadi
SIRT1 is an NAD+ -dependent deacetylase that acts as a nutrient sensitive regulator of longevity. SIRT1 also acts as a key regulator of mesenchymal stem cells (MSCs), adult stem cells that give rise to tissues such as bone, fat, muscle and cartilage. This review focuses on how SIRT1 regulates the self-renewal, multipotency and differentiation of MSCs. The variable role of SIRT1 in promoting the differentiation of MSCs towards certain lineages, while repressing others, will be examined within the broader context of aging, calorie restriction, and regenerative medicine...
April 2018: Regenerative Medicine
Sana H Surani, Aaron D Levine
AIM: To examine how the geographic distribution of pluripotent and adult stem cell research publications within the USA differs from other areas of biomedical research. MATERIALS & METHODS: Publication count data for pluripotent stem cell research, adult stem cell research and a comparison group representative of biomedical research more broadly were collected and analyzed for each US state from 2009 to 2016. RESULTS: The distribution of pluripotent stem cell research differed from the other fields with overperformance in pluripotent stem cell research observed in California, as well as Wisconsin, Massachusetts, Maryland and Connecticut...
April 2018: Regenerative Medicine
Yang Song, Hui Du, Chengxiang Dai, Li Zhang, Suke Li, David J Hunter, Liangjing Lu, Chunde Bao
AIM: This study aimed to evaluate the safety and therapeutic potential of autologous human adipose-derived mesenchymal stem cells (haMSCs) in patients with osteoarthritis. MATERIALS & METHODS: Safety and efficacy of haMSCs were preclinically assessed in vitro and in BALB/c-nu nude mice. 18 patients were enrolled and divided into three dose groups: the low-dose, mid-dose and high-dose group (1 × 107 , 2 × 107 and 5 × 107 cells, respectively), provided three injections and followed up for 96 weeks...
April 2018: Regenerative Medicine
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