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Biointerphases

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https://www.readbyqxmd.com/read/28768420/sputter-coated-zno-thin-films-on-glass-and-polycarbonate-evaluation-of-stability-and-interaction-with-flavin-adenine-dinucleotide-dependent-oxidases
#1
Fidal Vallam Thodi, Abhilash Thendiyammal, Balasubrahmaniyam Mukundakumar, Kasiviswanathan Subbiah, Chandra T Sainathan
Aqueous stability of sputter coated ZnO thin films were studied on two base materials, viz., polycarbonate (PC) and glass. The films showed higher stability on PC compared to glass, when exposed to aqueous buffered solution at pH-7.4, as studied by x-ray diffraction, surface reflectometry, and inductively coupled plasma-optical emission spectroscopy. Glucose oxidase (GOx) and cholesterol oxidase (Chl.Ox.) were used as model enzymes to study their electrochemical interaction with ZnO/PC. GOx showed a higher immobilization on ZnO/PC with an activity of 9...
August 2, 2017: Biointerphases
https://www.readbyqxmd.com/read/28768419/lipid-droplets-exhaustion-with-caspases-activation-in-hela-cells-cultured-in-plasma-activated-medium-observed-by-multiplex-coherent-anti-stokes-raman-scattering-microscopy
#2
Ryo Furuta, Naoyuki Kurake, Keigo Takeda, Kenji Ishikawa, Takayuki Ohta, Masafumi Ito, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, Masaru Hori
The multiplex coherent anti-Stokes Raman scattering microscopy allowed label-free visualization of cytoplasmic lipid droplets (LDs). The LDs, which act to conserve energy storage, are usually accumulated during the normal apoptosis of HeLa cells with activation of caspase-3/7 leading to downregulation of the fatty acid catabolism pathways. During cultivating in nonthermal plasma-activated medium (PAM), while the activation of caspase-3/7 was induced, the authors found that a dynamic exhaustion of the intracellular LDs, underlying the metabolic mechanism of the PAM-induced apoptotic cell death of HeLa cells...
August 2, 2017: Biointerphases
https://www.readbyqxmd.com/read/28754039/nanodiamond-chymotrypsin-and-nanodiamond-papain-conjugates-their-synthesis-and-activity-and-visualization-of-their-interaction-with-cells-using-optical-and-electron-microscopy
#3
Sergey A Golyshev, Anna K Berkovich, Ruslan Yu Yakovlev, Dmitry M Bystrov, Nikita M Ivanov, Galina N Balandina, Galina N Rudenskaya
Two novel conjugates of detonation nanodiamonds (dNDs) with the proteolytic enzymes chymotrypsin and papain were synthesized. The synthesis was performed via functionalization of the dNDs' surface with acidic/alkali treatment followed by carbodiimide-mediated protein binding. Covalent binding of the enzymes was confirmed by Fourier transform infrared spectrographic analysis and high-performance liquid chromatography (HPLC) amino acid analysis. HPLC also proved the preservation of the enzymes' composition during synthesis...
July 28, 2017: Biointerphases
https://www.readbyqxmd.com/read/28750541/influence-of-different-cleaning-processes-on-the-surface-chemistry-of-gold-nanoparticles
#4
Rita La Spina, Valentina Spampinato, Douglas Gilliland, Isaac Ojea-Jimenez, Giacomo Ceccone
In this paper, the authors have investigated the effects of different cleaning methods (centrifugation and dialysis) on the surface chemistry and composition of 15 nm sodium citrate stabilized gold nanoparticles. The nuclear magnetic resonance (NMR) results indicate that three centrifugation cycles are sufficient to remove most of the citrate molecules, while centrifuged liquid sedimentation and dynamic light scattering data reveal some degree of nanoparticle aggregation when three centrifugation cycles are exceeded...
July 27, 2017: Biointerphases
https://www.readbyqxmd.com/read/28704999/multilayer-poly-3-4-ethylenedioxythiophene-dexamethasone-and-poly-3-4-ethylenedioxythiophene-polystyrene-sulfonate-carbon-nanotubes-coatings-on-glassy-carbon-microelectrode-arrays-for-controlled-drug-release
#5
Elisa Castagnola, Stefano Carli, Maria Vomero, Alice Scarpellini, Mirko Prato, Noah Goshi, Luciano Fadiga, Sam Kassegne, Davide Ricci
The authors present an electrochemically controlled, drug releasing neural interface composed of a glassy carbon (GC) microelectrode array combined with a multilayer poly(3,4-ethylenedioxythiophene) (PEDOT) coating. The system integrates the high stability of the GC electrode substrate, ideal for electrical stimulation and electrochemical detection of neurotransmitters, with the on-demand drug-releasing capabilities of PEDOT-dexamethasone compound, through a mechanically stable interlayer of PEDOT-polystyrene sulfonate (PSS)-carbon nanotubes (CNT)...
July 13, 2017: Biointerphases
https://www.readbyqxmd.com/read/28693327/in-situ-examination-of-osteoblast-biomineralization-on-sulfonated-polystyrene-modified-substrates-using-fourier-transform-infrared-microspectroscopy
#6
Yizhi Meng, Meghan E Faillace, Kathryn Dorst, Samantha J Palmaccio, Lisa M Miller, Yi-Xian Qin
Osteoporosis is a skeletal disorder that is characterized by the loss of bone mineral density (BMD) resulting in increased risk of fracture. However, it has been shown that BMD is not the only indicator of fracture risk, as the strength of bone depends on a number of factors, including bone mass, architecture and material properties. Physiological mineral deposition requires the formation of a properly developed extracellular matrix (ECM), which recruits calcium and phosphate ions into the synthesis of apatite crystals...
July 10, 2017: Biointerphases
https://www.readbyqxmd.com/read/28683556/water-contact-angle-is-not-a-good-predictor-of-biological-responses-to-materials
#7
Morgan R Alexander, Paul Williams
Often the view is expressed that water contact angle (WCA) or other wettability/surface energy measurements made on a material surface can be used to predict cellular attachment to materials, e.g., bacteria attach to hydrophobic surfaces. In this article, the authors present a perspective emerging from their work that has failed to find relationships between WCA and microbial and stem cell attachment within large diversity material libraries and compare with the literature concluding that such simple rules are (unfortunately) wholly inadequate to explain cell-material interactions...
July 6, 2017: Biointerphases
https://www.readbyqxmd.com/read/28658958/collagen-affinity-coating-for-surface-binding-of-decorin-and-other-biomolecules-surface-characterization
#8
Marisa L Sylvester, Buddy D Ratner
The development of biomaterials that promote tissue reconstruction and regeneration can reduce the low level, chronic inflammation and encapsulation that impact the performance of today's medical devices. Specifically, in the case of implantable sensors, the host response often leads to poor device performance that discourages permanent implantation. Our goal is to present on medical implants bioactive molecules that can promote healing rather than scarring. Localized delivery of these molecules would also minimize the possibility of adverse tissue reactions elsewhere in the body...
June 28, 2017: Biointerphases
https://www.readbyqxmd.com/read/28658957/preface-in-focus-issue-on-protein-structures-at-biointerfaces
#9
Tobias Weidner
No abstract text is available yet for this article.
June 28, 2017: Biointerphases
https://www.readbyqxmd.com/read/28655279/solid-state-deuterium-nmr-study-of-lk%C3%AE-14-peptide-aggregation-in-biosilica
#10
Helen E Ferreira, Gary P Drobny
In nature, organisms including diatoms, radiolaria, and marine sponges use proteins, long chain polyamines, and other organic molecules to regulate the assembly of complex silica-based structures. Here, the authors investigate structural features of small peptides, designed to mimic the silicifying activities of larger proteins found in natural systems. LKα14 (Ac-LKKLLKLLKKLLKL-C), an amphiphilic lysine/leucine repeat peptide with an α-helical secondary structure at polar/apolar interfaces, coprecipitates with silica to form nanospheres...
June 27, 2017: Biointerphases
https://www.readbyqxmd.com/read/28637352/absence-of-conformational-change-in-complement-factor-3-and-factor-xii-adsorbed-to-acrylate-polymers-is-related-to-a-high-degree-of-polymer-backbone-flexibility
#11
Karin Fromell, Yi Yang, Kristina Nilsson Ekdahl, Bo Nilsson, Mattias Berglin, Hans Elwing
In previous investigations, the authors have examined the adsorption of albumin, immunoglobulin, and fibrinogen to a series of acrylate polymers with different backbone and side-group flexibility. The authors showed that protein adsorption to acrylates with high flexibility, such as poly(lauryl methacrylate) (PLMA), tends to preserve native conformation. In the present study, the authors have continued this work by examining the conformational changes that occur during the binding of complement factor 3 (C3) and coagulation factor XII (FXII)...
June 21, 2017: Biointerphases
https://www.readbyqxmd.com/read/28614950/grafting-of-architecture-controlled-poly-styrene-sodium-sulfonate-onto-titanium-surfaces-using-bio-adhesive-molecules-surface-characterization-and-biological-properties
#12
Hamza Chouirfa, Margaret D M Evans, David G Castner, Penny Bean, Dimitri Mercier, Anouk Galtayries, Céline Falentin-Daudré, Véronique Migonney
This contribution reports on grafting of bioactive polymers such as poly(sodium styrene sulfonate) (polyNaSS) onto titanium (Ti) surfaces. This grafting process uses a modified dopamine as an anchor molecule to link polyNaSS to the Ti surface. The grafting process combines reversible addition-fragmentation chain transfer polymerization, postpolymerization modification, and thiol-ene chemistry. The first step in the process is to synthetize architecture controlled polyNaSS with a thiol end group. The second step is the adhesion of the dopamine acrylamide (DA) anchor onto the Ti surfaces...
June 14, 2017: Biointerphases
https://www.readbyqxmd.com/read/28614949/comparison-of-metrological-techniques-for-evaluation-of-the-impact-of-a-cosmetic-product-containing-hyaluronic-acid-on-the-properties-of-skin-surface
#13
Rahula Janiš, Vladimír Pata, Pavlína Egner, Jana Pavlačková, Andrea Zapletalová, Kristina Kejlová
The aim of this research was to evaluate mutual interchangeability of four principally different biometric instrumental techniques designed for objective measurement of changes in the physical, mechanical, and topographical properties of the skin surface treated with commercial antiaging cosmetic products with hyaluronic acid. The following instrumental devices were used: Visioscope PC 35, Corneometer Multiprobe Adapter MPA 6, Reviscometer RVM 600, and 3D scanner Talysurf CLI 500. The comparison of the individual methods was performed using cluster analysis...
June 14, 2017: Biointerphases
https://www.readbyqxmd.com/read/28610429/optimization-of-electrospun-poly-n-isopropyl-acrylamide-mats-for-the-rapid-reversible-adhesion-of-mammalian-cells
#14
Kirsten N Cicotte, Jamie A Reed, Phuong Anh H Nguyen, Jacqueline A De Lora, Elizabeth L Hedberg-Dirk, Heather E Canavan
Poly(N-isopropyl acrylamide) (pNIPAM) is a "smart" polymer that responds to changes in altering temperature near physiologically relevant temperatures, changing its relative hydrophobicity. Mammalian cells attach to pNIPAM at 37 °C and detach spontaneously as a confluent sheet when the temperature is shifted below the lower critical solution temperature (∼32 °C). A variety of methods have been used to create pNIPAM films, including plasma polymerization, self-assembled monolayers, and electron beam ionization...
June 13, 2017: Biointerphases
https://www.readbyqxmd.com/read/28605919/happy-70th-birthday-to-buddy-ratner
#15
Allan S Hoffman
No abstract text is available yet for this article.
June 12, 2017: Biointerphases
https://www.readbyqxmd.com/read/28592113/ph-dependent-lipid-vesicle-interactions-with-plasma-polymerized-thin-films
#16
Hannah J Askew, Mirren Charnley, Karyn L Jarvis, Sally L McArthur
Model lipid vesicle and supported lipid bilayer (SLB) systems are used in a variety of applications including biosensing, cell membrane mimics, and drug delivery. Exposure of a surface to a vesicle solution provides a straightforward method for creating such systems via vesicle adsorption and collapse. However, this process is complex and the relationship between the surface physicochemical properties and vesicle collapse is poorly understood. Plasma polymers are thin conformal films that can be applied to a variety of materials to modify surface properties...
June 7, 2017: Biointerphases
https://www.readbyqxmd.com/read/28592112/characterization-and-cellular-studies-of-molecular-nanoparticle-of-iron-iii-tannic-complexes-toward-a-low-cost-magnetic-resonance-imaging-agent
#17
Saowalak Krungchanuchat, Titipun Thongtem, Somchai Thongtem, Chalermchai Pilapong
Herein, a new magnetic resonance imaging (MRI) agent based on molecular nanoparticles of iron(III)-tannic complexes (Fe-TA NPs) is reported. The paramagnetic and molecularlike Fe-TA NPs were successfully synthesized at room temperature within a few minutes without the use of any toxic agents or expensive equipment. The coordination states of the Fe-TA NPs were pH-dependent. The r1 relaxivity values of the bis-dominated and tris-dominated structures of the Fe-TA NPs were determined to be 6.31 and 5.24 mM(-1 )s(-1), respectively, by using a Philips Achieva 1...
June 7, 2017: Biointerphases
https://www.readbyqxmd.com/read/28587470/surface-engineering-of-poly-methylmethacrylate-effects-on-fluorescence-immunoassay
#18
Peter W Akers, Nam Cao Hoai Le, Andrew R J Nelson, Milena McKenna, Christy O'Mahony, Duncan J McGillivray, Vladimir Gubala, David E Williams
The authors present surface engineering modifications through chemistry of poly(methylmethacrylate) (PMMA) that have dramatic effects on the result of surface-bound fluorescence immunoassays, both for specific and nonspecific signals. The authors deduce the most important effect to be clustering of antibodies on the surface leading to significant self-quenching. Secondary effects are attributable to the formation of sparse multilayers of antibody. The authors compare PMMA as an antibody support surface with ultraviolet-ozone oxidized PMMA and also to substrates that were, after the oxidation, surface modified by a four-unit poly(ethyleneglycol) carboxylic acid (PEG4), a branched tricarboxylic acid, and a series of carboxylic acid-terminated dendrimers, from generation 1...
June 6, 2017: Biointerphases
https://www.readbyqxmd.com/read/28576080/lysozyme-orientation-and-conformation-on-mos2-surface-insights-from-molecular-simulations
#19
Hongjie Fan, Daohui Zhao, Yingtu Li, Jian Zhou
Two-dimensional molybdenum disulfide (MoS2) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS2 and biological molecules such as proteins, but little knowledge exists on the orientation and conformation of proteins on the MoS2 surface at the molecular level. In this work, the lysozyme adsorption on the MoS2 surface was studied by molecular dynamics simulations, wherein six different orientations were selected based on the different faces of lysozyme...
June 2, 2017: Biointerphases
https://www.readbyqxmd.com/read/28571325/poly-ethylene-glycol-hydrogel-based-on-oxa-michael-reaction-precursor-synthesis-and-hydrogel-formation
#20
Hanqi Wang, Fang Cheng, Wei He, Jiaohui Zhu, Gang Cheng, Jingping Qu
This paper reported a facile strategy for the one-pot synthesis of vinyl sulfone (VS) group terminated hydrogel precursors [poly(ethylene) glycol (PEG)-VS] and PEG hydrogels via catalytic oxa-Michael reaction. Nine potential catalysts were investigated for the reaction between PEG and divinyl sulfone, among which 4-dimethylaminopyridine (DMAP) prevailed for its high catalytic activity. DMAP produced PEG-VS with a conversion of more than 90% in 2 h under a solvent-free condition at room temperature, which significantly simplifies the synthesis of PEG-VS...
June 1, 2017: Biointerphases
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