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Shusuke Nakano, Takayuki Yamagishi, Satoka Aoyagi, André Portz, Michael Dürr, Hideo Iwai, Tomoko Kawashima
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is one of the most powerful methods to analyze biomolecules in biological tissues and cells because it provides detailed chemical structure information and chemical images with a high spatial resolution. However, in terms of quantitative analysis, there are issues such as matrix effects that often cause secondary ion intensity changes regardless of the actual concentration in a sample. For instance, the intensity of secondary ions related to peptides is generally suppressed when lipids coexist...
January 19, 2018: Biointerphases
Yaokun Pan, Chuanzhong Chen, Rui Feng, Hongwei Cui, Benkui Gong, Tingting Zheng, Yarou Ji
Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepared on Mg-xCa alloys to study the effect of Ca on the microstructure and corrosion resistance of Mg-xCa alloys and their surface MAO coatings. The electrochemical corrosion behavior was investigated using an electrochemical workstation, and the degradability and bioactivity were evaluated by soaking tests in simulated body fluid (SBF) solutions...
January 16, 2018: Biointerphases
Marwa Munem, Oscar Zaar, Kelly Dimovska Nilsson, Noora Neittaanmäki, John Paoli, John S Fletcher
A set of basal cell carcinoma samples, removed by Mohs micrographic surgery and pathologically identified as having an aggressive subtype, have been analyzed using time-of-flight secondary ion mass spectrometry (SIMS). The SIMS analysis employed a gas cluster ion beam (GCIB) to increase the sensitivity of the technique for the detection of intact lipid species. The GCIB also allowed these intact molecular signals to be maintained while surface contamination and delocalized chemicals were removed from the upper tissue surface...
January 12, 2018: Biointerphases
Lin Du, Xiaohui Yang, Wenqiang Li, Haoying Li, Shanbao Feng, Rong Zeng, Bin Yu, Liangxing Xiao, Yu Liu, Mei Tu, Heng-Yong Nie
As an antibiotic that prevents and treats infections caused by Gram-positive bacteria such as Staphylococcus aureus, vancomycin incorporated in a biodegradable polymer poly(lactide-co-glycolide) provides opportunities to construct controlled-release drug delivery systems. Developments associated with this promising system have been largely concentrated on areas of drug delivery kinetics and biodegradability. In order to provide surface analytical approaches to this important system, the authors demonstrate applicability of time-of-flight secondary ion mass spectrometry (TOF-SIMS) in three-dimensional molecular imaging for a model system consisting of alternating layers of ploy(lactide-co-glycolide) and vancomycin...
January 10, 2018: Biointerphases
Patricia Losada-Pérez, Ozge Polat, Atul N Parikh, Erkin Seker, Frank Uwe Renner
Nanoporous gold (np-Au) is a nanostructured metal with many desirable attributes. Despite the growing number of applications of nanoporous materials, there are still open questions regarding their fabrication and subsequent surface functionalization. For example, the hydrophobic nature of gold surfaces makes the formation of planar supported lipid layers challenging. Here, the authors engineer the interface between np-Au and 1,2-dioleoyl-sn-glycero-3-phosphocholine lipid layers using well-differentiated approaches based on vesicle adsorption and solvent exchange methods...
January 5, 2018: Biointerphases
Katharina Maniura-Weber, Stefan Zauscher, Sally McArthur
No abstract text is available yet for this article.
January 4, 2018: Biointerphases
Caitlyn M Gatley-Montross, John A Finlay, Nick Aldred, Harrison Cassady, Joel F Destino, Beatriz Orihuela, Michael A Hickner, Anthony S Clare, Daniel Rittschof, Eric R Holm, Michael R Detty
Multivariate analyses were used to investigate the influence of selected surface properties (Owens-Wendt surface energy and its dispersive and polar components, static water contact angle, conceptual sign of the surface charge, zeta potentials) on the attachment patterns of five biofouling organisms (Amphibalanus amphitrite, Amphibalanus improvisus, Bugula neritina, Ulva linza, and Navicula incerta) to better understand what surface properties drive attachment across multiple fouling organisms. A library of ten xerogel coatings and a glass standard provided a range of values for the selected surface properties to compare to biofouling attachment patterns...
December 29, 2017: Biointerphases
Jun Ogi, Yuri Kato, Yoshihisa Matoba, Chigusa Yamane, Kazunori Nagahata, Yusaku Nakashima, Takuya Kishimoto, Shigeki Hashimoto, Koichi Maari, Yusuke Oike, Takayuki Ezaki
A 24-μm-pitch microelectrode array (MEA) with 6912 readout channels at 12 kHz and 23.2-μVrms random noise is presented. The aim is to reduce noise in a "highly scalable" MEA with a complementary metal-oxide-semiconductor integration circuit (CMOS-MEA), in which a large number of readout channels and a high electrode density can be expected. Despite the small dimension and the simplicity of the in-pixel circuit for the high electrode-density and the relatively large number of readout channels of the prototype CMOS-MEA chip developed in this work, the noise within the chip is successfully reduced to less than half that reported in a previous work, for a device with similar in-pixel circuit simplicity and a large number of readout channels...
December 19, 2017: Biointerphases
Richard P Harrison, Veeren M Chauhan
Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to wide-scale adoption of CGTs remains challenging, such that the emergence of a "blockbuster" therapy has so far proved elusive. Manufacturing solutions for these therapies require the application of scalable and replicable cell manufacturing techniques, which differ markedly from the existing pharmaceutical incumbent...
December 15, 2017: Biointerphases
Sally L McArthur, Katharina Maniura
No abstract text is available yet for this article.
December 15, 2017: Biointerphases
Abdulhalim Kılıç, Fatma Nese Kok
Quartz crystal microbalance with dissipation (QCM-D) is one of the powerful techniques, which allow real time, quantitative and noninvasive analysis of the interaction of different cell types with various modified surfaces. In this study, the dynamic adhesion behavior of human fetal osteoblastic bone (hfOB) cell lines was first monitored on untreated and hydrophilically treated gold sensor surfaces as reference substrates. Adhesion was also observed under light microscopy to facilitate the evaluation. Cells increased their surface contact area and spread more on hydrophilic surfaces, and showed distinct profile with an increased rigidity at the interfacial layer, which is assigned to extracellular matrix remodeling...
December 12, 2017: Biointerphases
Oswald Prucker, Thomas Brandstetter, Jürgen Rühe
A novel method for the generation of surface-attached hydrogel coatings and their use in biomedical applications is discussed. This short review concentrates on surface architectures that are prepared from prepolymers carrying reactive groups suitable for crosslinking via C,H insertion reactions [C,H insertion crosslinking (CHic)]. Upon photochemical or thermal activation these groups do not only induce the crosslinking of the system, but also connect the forming gel to the surface as long as the surface itself consists of an organic material...
December 8, 2017: Biointerphases
Khulan Sergelen, Christian Petri, Ulrich Jonas, Jakub Dostalek
The preparation and investigation of a free-standing membrane made from a composite of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) and polystyrene nanoparticles (PS NP) with temperature-controlled permeability is reported. The method exploits the light-induced crosslinking of the photo-reactive pNIPAAm-based polymer and mechanical reinforcement of the membrane structure by the polystyrene nanoparticles. About micrometer thick layers were either directly attached to a gold surface or prepared as free-standing layers spanning over arrays of microfluidic channels with a width of about hundred microns by using template stripping...
December 6, 2017: Biointerphases
Alexander Heiss, Birger Freisinger, Evi Held-Föhn
The oxidation based antimicrobial activity of silver is long known. Microparticles with a particular silver-ruthenium coating and specific physical properties were developed. The coating showed a considerably increased silver ion release rate in comparison to a plain silver coating. Accordingly, an exposure of Escherichia coli and Staphylococcus aureus to these silver-ruthenium coated microparticles resulted in a time and concentration dependent cell killing. Even though contact killing may contribute to this efficacy, rather a release associated diffusion gradient dependent killing was observed...
December 5, 2017: Biointerphases
Troels Røn, Navin J Patil, Fatemeh Ajalloueian, Sankaranarayanan Rishikesan, Bruno Zappone, Ioannis S Chronakis, Seunghwan Lee
Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the rheological properties. This study investigates both rheological and tribological shear properties of thin films of gastric mucus from a porcine source and its mimics at compliant soft interfaces...
December 5, 2017: Biointerphases
Haishan Shi, Jing Zhang, Xiaoling Ye, Tingting Wu, Jiandong Ye
Biological apatite is ion-doped and provides an active pool for the exchange with foreign impurity ions. In this work, an in vitro model of hydrated metastable octacalcium phosphate (OCP) crystals was established to mimetically investigate the distinct exchange of trivalent and divalent cations (Fe(3+) and Sr(2+)) with biological apatite. Fe(3+) significantly promoted the collapses of OCP crystals and the formation of amorphous sol-like ferric phosphates, while Sr(2+) facilitated the epitaxial growth and stability of OCP crystals...
November 21, 2017: Biointerphases
Laura J Domigan, Helen Ashmead, Simone Dimartino, Jenny Malmstrom, F Grant Pearce, Matthew Blunt, David E Williams, Juliet A Gerrard
Recent research has highlighted the exciting possibilities enabled by the use of protein structures as nanocomponents to form functional nanodevices. To this end, control over protein-protein and protein-surface interactions is essential. In this study, the authors probe the interaction of human peroxiredoxin 3 with gold surfaces, a protein that has been previously identified as having potential use in nanotechnology. Analytical ultracentrifugation and transmission electron microscopy revealed the pH mediated assembly of protein toroids into tubular structures across a small pH range...
November 15, 2017: Biointerphases
Dajana Tanasic, Adriana Rathner, Jan Philipp Kollender, Petr Rathner, Norbert Müller, Karl Christian Zelenka, Achim Walter Hassel, Cezarina Cela Mardare
Developing novel compounds with antimicrobial properties can be an effective approach to decreasing the number of healthcare-associated infections, particularly in the context of medical devices and touch surfaces. A variety of molybdate powders (Ag2MoO4, CaMoO4, CuMoO4 and Cu3Mo2O9) were synthesized and characterized, and Escherichia coli was used as a model gram-negative bacterium to demonstrate their antimicrobial properties. Optical density measurements, bacterial colony growth, and stained gel images for protein expression clearly showed that silver- and copper molybdates inhibit bacterial growth, whereas CaMoO4 exhibited no bactericidal effect...
November 7, 2017: Biointerphases
Neda Davoudi, Katharina Huttenlochner, Jonas Chodorski, Christin Schlegel, Martin Bohley, Christine Müller-Renno, Jan C Aurich, Roland Ulber, Christiane Ziegler
The bacterial attachment to surfaces is the first step of biofilm formation. This attachment is governed by adhesion forces which act between the bacterium and the substrate. Such forces can be measured by single cell force spectroscopy, where a single bacterium is attached to a cantilever of a scanning force microscope, and force-distance curves are measured. For the productive sea-water bacterium Paracoccus seriniphilus, pH dependent measurements reveal the highest adhesion forces at pH 4. Adhesion forces measured at salinities between 0% and 4...
November 6, 2017: Biointerphases
Per Malmberg, Narmin Bigdeli, Jens Jensen, Håkan Nygren
The bone material interface has been an area of intense study over many decades, where studies of the healing process ranging from simple mineral deposition in vitro to actual healing in vivo have given important clues to the importance of calcium minerals in the bone/implant interface. Here, the authors use a combination of in vitro cell culture methods and in vivo implantation to study how the role of the spontaneously formed hydroxyapatite layer on Ti-implants for the in vivo-healing into the bone tissue of rat tibia...
October 27, 2017: Biointerphases
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