Read by QxMD icon Read

Metabolomics: Official Journal of the Metabolomic Society

Hailong Zhang, Longzhen Cui, Wen Liu, Zhenfeng Wang, Yang Ye, Xue Li, Huijuan Wang
Introduction: Gastric cancer (GC) is a malignant tumor worldwide. As primary pathway for metastasis, the lymphatic system is an important prognostic factor for GC patients. Although the metabolic changes of gastric cancer have been investigated in extensive studies, little effort focused on the metabolic profiling of lymph node metastasis (LNM)-positive or negative GC patients. Objectives: We performed1 H NMR spectrum of GC tissue samples with and without LNM to identify novel potential metabolic biomarkers in the process of LNM of GC...
2018: Metabolomics: Official Journal of the Metabolomic Society
Parastoo Fazelzadeh, Roland W J Hangelbroek, Peter J Joris, Casper G Schalkwijk, Diederik Esser, Lydia Afman, Thomas Hankemeier, Doris M Jacobs, Velitchka V Mihaleva, Sander Kersten, John van Duynhoven, Mark V Boekschoten
Introduction: The response to dietary challenges has been proposed as a more accurate measure of metabolic health than static measurements performed in the fasted state. This has prompted many groups to explore the potential of dietary challenge tests for assessment of diet and lifestyle induced shifts in metabolic phenotype. Objectives: We examined whether the response to a mixed-meal challenge could provide a readout for a weight loss (WL)-induced phenotype shift in abdominally obese male subjects...
2018: Metabolomics: Official Journal of the Metabolomic Society
Marek J Noga, Ronald Zielman, Robin M van Dongen, Sabine Bos, Amy Harms, Gisela M Terwindt, Arn M J M van den Maagdenberg, Thomas Hankemeier, Michel D Ferrari
Introduction: Metabolic profiling of cerebrospinal fluid (CSF) is a promising technique for studying brain diseases. Measurements should reflect the in vivo situation, so ex vivo metabolism should be avoided. Objective: To investigate the effects of temperature (room temperature vs. 4 °C), centrifugation and ethanol, as anti-enzymatic additive during CSF sampling on concentrations of glutamic acid, glutamine and other endogenous amines. Methods: CSF samples from 21 individuals were processed using five different protocols...
2018: Metabolomics: Official Journal of the Metabolomic Society
Antonio Rosato, Leonardo Tenori, Marta Cascante, Pedro Ramon De Atauri Carulla, Vitor A P Martins Dos Santos, Edoardo Saccenti
Introduction: Metabolomics is a well-established tool in systems biology, especially in the top-down approach. Metabolomics experiments often results in discovery studies that provide intriguing biological hypotheses but rarely offer mechanistic explanation of such findings. In this light, the interpretation of metabolomics data can be boosted by deploying systems biology approaches. Objectives: This review aims to provide an overview of systems biology approaches that are relevant to metabolomics and to discuss some successful applications of these methods...
2018: Metabolomics: Official Journal of the Metabolomic Society
Abdul-Hamid Emwas, Edoardo Saccenti, Xin Gao, Ryan T McKay, Vitor A P Martins Dos Santos, Raja Roy, David S Wishart
1 H NMR spectra from urine can yield information-rich data sets that offer important insights into many biological and biochemical phenomena. However, the quality and utility of these insights can be profoundly affected by how the NMR spectra are processed and interpreted. For instance, if the NMR spectra are incorrectly referenced or inconsistently aligned, the identification of many compounds will be incorrect. If the NMR spectra are mis-phased or if the baseline correction is flawed, the estimated concentrations of many compounds will be systematically biased...
2018: Metabolomics: Official Journal of the Metabolomic Society
Eugenio Alcalde, Paul D Fraser
Introduction: Xanthophyllomyces dendrorhous is a non-conventional industrial yeast. It has the unique ability among yeasts to produce geranylgeranyl pyrophosphate derived terpenoids such as carotenoids and in particular the high value pigment astaxanthin. Objective: In order to fully exploit the industrial potential of Xanthophyllomyces using modern industrial biotechnology approaches the further development of "omic" resources in this organism are required to build on the now sequenced and annotated genome...
2018: Metabolomics: Official Journal of the Metabolomic Society
Rachel A Spicer, Christoph Steinbeck
Introduction: Data sharing is being increasingly required by journals and has been heralded as a solution to the 'replication crisis'. Objectives: (i) Review data sharing policies of journals publishing the most metabolomics papers associated with open data and (ii) compare these journals' policies to those that publish the most metabolomics papers. Methods: A PubMed search was used to identify metabolomics papers. Metabolomics data repositories were manually searched for linked publications...
2018: Metabolomics: Official Journal of the Metabolomic Society
Margaux Luck, Caroline Schmitt, Neila Talbi, Laurent Gouya, Cédric Caradeuc, Hervé Puy, Gildas Bertho, Nicolas Pallet
Introduction: Metabolomic profiling combines Nuclear Magnetic Resonance spectroscopy with supervised statistical analysis that might allow to better understanding the mechanisms of a disease. Objectives: In this study, the urinary metabolic profiling of individuals with porphyrias was performed to predict different types of disease, and to propose new pathophysiological hypotheses. Methods: Urine 1H-NMR spectra of 73 patients with asymptomatic acute intermittent porphyria (aAIP) and familial or sporadic porphyria cutanea tarda (f/sPCT) were compared using a supervised rule-mining algorithm...
2018: Metabolomics: Official Journal of the Metabolomic Society
Rachael Hough, Debra Archer, Christopher Probert
Introduction: Disturbance to the hindgut microbiota can be detrimental to equine health. Metabolomics provides a robust approach to studying the functional aspect of hindgut microorganisms. Sample preparation is an important step towards achieving optimal results in the later stages of analysis. The preparation of samples is unique depending on the technique employed and the sample matrix to be analysed. Gas chromatography mass spectrometry (GCMS) is one of the most widely used platforms for the study of metabolomics and until now an optimised method has not been developed for equine faeces...
2018: Metabolomics: Official Journal of the Metabolomic Society
Sven Zukunft, Cornelia Prehn, Cornelia Röhring, Gabriele Möller, Martin Hrabě de Angelis, Jerzy Adamski, Janina Tokarz
Introduction: Global metabolomics analyses using body fluids provide valuable results for the understanding and prediction of diseases. However, the mechanism of a disease is often tissue-based and it is advantageous to analyze metabolomic changes directly in the tissue. Metabolomics from tissue samples faces many challenges like tissue collection, homogenization, and metabolite extraction. Objectives: We aimed to establish a metabolite extraction protocol optimized for tissue metabolite quantification by the targeted metabolomics AbsoluteIDQ™ p180 Kit (Biocrates)...
2018: Metabolomics: Official Journal of the Metabolomic Society
Ruifang Li-Gao, Renée de Mutsert, Patrick C N Rensen, Jan Bert van Klinken, Cornelia Prehn, Jerzy Adamski, Astrid van Hylckama Vlieg, Martin den Heijer, Saskia le Cessie, Frits R Rosendaal, Ko Willems van Dijk, Dennis O Mook-Kanamori
Introduction: Fasting metabolite profiles have been shown to distinguish type 2 diabetes (T2D) patients from normal glucose tolerance (NGT) individuals. Objectives: We investigated whether, besides fasting metabolite profiles, postprandial metabolite profiles associated with T2D can stratify individuals with impaired fasting glucose (IFG) by their similarities to T2D. Methods: Three groups of individuals (age 45-65 years) without any history of IFG or T2D were selected from the Netherlands Epidemiology of Obesity study and stratified by baseline fasting glucose concentrations (NGT (n = 176), IFG (n = 186), T2D (n = 171))...
2018: Metabolomics: Official Journal of the Metabolomic Society
Josef Marksteiner, Imrich Blasko, Georg Kemmler, Therese Koal, Christian Humpel
Introduction: There is still a clear need for a widely available, inexpensive and reliable method to diagnose Alzheimer's disease (AD) and monitor disease progression. Liquid chromatography-mass spectrometry (LC-MS) is a powerful analytic technique with a very high sensitivity and specificity. Objectives: The aim of the present study is to measure concentrations of 20 bile acids using the novel Kit from Biocrates Life Sciences based on LC-MS technique. Methods: Twenty bile acid metabolites were quantitatively measured in plasma of 30 cognitively healthy subjects, 20 patients with mild cognitive impairment (MCI) and 30 patients suffering from AD...
2018: Metabolomics: Official Journal of the Metabolomic Society
Swathi Alagesan, Nigel P Minton, Naglis Malys
Introduction: Cupriavidus necator H16 is a gram-negative bacterium, capable of lithoautotrophic growth by utilizing hydrogen as an energy source and fixing carbon dioxide (CO2 ) through Calvin-Benson-Bassham (CBB) cycle. The potential to utilize synthesis gas (Syngas) and the prospects of rerouting carbon from polyhydroxybutyrate synthesis to value-added compounds makes C. necator an excellent chassis for industrial application. Objectives: In the context of lack of sufficient quantitative information of the metabolic pathways and to advance in rational metabolic engineering for optimized product synthesis in C...
2018: Metabolomics: Official Journal of the Metabolomic Society
Emily R Sekera, Heather L Rudolph, Stephen D Carro, Michael J Morales, Glenna C L Bett, Randall L Rasmusson, Troy D Wood
Introduction: Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders lacking a clinical biomarker for diagnosis. Emerging evidence shows that intestinal microflora from ASD subjects can be distinguished from controls, suggesting metabolite differences due to the action of intestinal microbes may provide a means for identifying potential biomarkers for ASD. Objectives: The aim of this study was to determine if quantitative differences in levels of stercobilin and stercobilinogen, metabolites produced by biological action of intestinal microflora, exist in the fecal matter between an ASD mouse model population and controls...
November 2017: Metabolomics: Official Journal of the Metabolomic Society
Santosh K Bharti, Flonné Wildes, Chien-Fu Hung, T C Wu, Zaver M Bhujwalla, Marie-France Penet
Introduction: Malignant ascites (MA) is a major cause of morbidity that occurs in 37% of ovarian cancer patients. The accumulation of MA in the peritoneal cavity due to cancer results in debilitating symptoms and extremely poor quality of life. There is an urgent unmet need to expand the understanding of MA to design effective treatment strategies, and to improve MA diagnosis. Objective: Our purpose here is to contribute to a better characterization of MA metabolic composition in ovarian cancer...
October 2017: Metabolomics: Official Journal of the Metabolomic Society
Anita H Lewin, Peter Silinski, James Hayes, Amanda Gilbert, S Wayne Mascarella, Herbert H Seltzman
Introduction: Metabolomics analysis depends on the identification and validation of specific metabolites. This task is significantly hampered by the absence of well-characterized reference standards. The one-carbon carrier 10-formyltetrahydrofolate acts as a donor of formyl groups in anabolism where it is a substrate in formyltransferase reactions in purine biosynthesis. It has been reported as an unstable substance and is currently unavailable as a reference standard for metabolomics analysis...
October 2017: Metabolomics: Official Journal of the Metabolomic Society
Keedrian I Olmstead, Michael R La Frano, Johannes Fahrmann, Dmitry Grapov, Jose A Viscarra, John W Newman, Oliver Fiehn, Daniel E Crocker, Fabian V Filipp, Rudy M Ortiz
INTRODUCTION: Prolonged fasting in northern elephant seals (NES) is characterized by a reliance on lipid metabolism, conservation of protein, and reduced plasma insulin. During early fasting, glucose infusion previously reduced plasma free fatty acids (FFA); however, during late-fasting, it induced an atypical elevation in FFA despite comparable increases in insulin during both periods suggestive of a dynamic shift in tissue responsiveness to glucose-stimulated insulin secretion. OBJECTIVE: To better assess the contribution of insulin to this fasting-associated shift in substrate metabolism...
May 2017: Metabolomics: Official Journal of the Metabolomic Society
Susanne B Breitkopf, Stéphane J H Ricoult, Min Yuan, Ying Xu, David A Peake, Brendan D Manning, John M Asara
INTRODUCTION: Advances in high-resolution mass spectrometry have created renewed interest for studying global lipid biochemistry in disease and biological systems. OBJECTIVES: Here, we present an untargeted 30 min. LC-MS/MS platform that utilizes positive/negative polarity switching to perform unbiased data dependent acquisitions (DDA) via higher energy collisional dissociation (HCD) fragmentation to profile more than 1000-1500 lipid ions mainly from methyl-tert-butyl ether (MTBE) or chloroform:methanol extractions...
March 2017: Metabolomics: Official Journal of the Metabolomic Society
Matthew F Buas, Haiwei Gu, Danijel Djukovic, Jiangjiang Zhu, Lynn Onstad, Brian J Reid, Daniel Raftery, Thomas L Vaughan
INTRODUCTION/OBJECTIVES: Incidence of esophageal adenocarcinoma (EA), an often fatal cancer, has increased sharply over recent decades. Several important risk factors (reflux, obesity, smoking) have been identified for EA and its precursor, Barrett's esophagus (BE), but a key challenge remains identifying individuals at highest risk, since most with reflux do not develop BE, and most with BE do not progress to cancer. Metabolomics represents an emerging approach for identifying novel biomarkers associated with cancer development...
March 2017: Metabolomics: Official Journal of the Metabolomic Society
K Sattler, M Behnes, C Barth, A Wenke, B Sartorius, I El-Battrawy, K Mashayekhi, J Kuschyk, U Hoffmann, T Papavasiliu, C Fastner, S Baumann, S Lang, X Zhou, G Yücel, M Borggrefe, I Akin
Background: Left atrial appendage (LAA) closure (LAAC) by implantation of an occlusion device is an established cardiac intervention to reduce risk of stroke while avoiding intake of oral anticoagulation medication during atrial fibrillation. Cardiac interventions can alter local or systemic gene and protein expression. Effects of LAAC on systemic metabolism have not been studied yet. Objectives: We aimed to study the effects of interventional LAAC on systemic metabolism...
2017: Metabolomics: Official Journal of the Metabolomic Society
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"