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Biotechnology Journal

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https://www.readbyqxmd.com/read/30221837/advances-and-future-perspectives-in-4d-bioprinting
#1
REVIEW
Nureddin Ashammakhi, Samad Ahadian, Fan Zengjie, Kasinan Suthiwanich, Farnaz Lorestani, Gorka Orive, Serge Ostrovidov, Ali Khademhosseini
Three-dimensionally printed constructs are static and do not recapitulate the dynamic nature of tissues. Four-dimensional (4D) bioprinting has emerged to include conformational changes in printed structures in a predetermined fashion using stimuli-responsive biomaterials and/or cells. The ability to make such dynamic constructs would enable us to fabricate tissue structures that can undergo morphological changes. Furthermore, other fields (bioactuation, biorobotics, and biosensing) will benefit from developments in 4D bioprinting...
September 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30221835/move-to-nano-arthrology-targeted-stimuli-responsive-nanomedicines-combat-adaptive-treatment-tolerance-att-of-rheumatoid-arthritis
#2
REVIEW
Lu Liu, Weisheng Guo, Xing-Jie Liang
Rheumatoid arthritis (RA) is one of the most popular chronic autoimmune diseases characterized with persistent synovial inflammation and bone destruction. Although considerable developments have been gained in clinical treatment of RA, the major drawback to RA therapy stems from the adaptive treatment tolerance (ATT) following the long-term drug use, which causes compromised efficacy, sustained drug dose increase, and severe adverse events. To address these challenges, it is of great significance to put forward innovative therapeutic approaches for RA treatment...
September 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30221832/enzymatic-adaptation-of-podospora-anserina-to-different-plant-biomass-provides-leads-to-optimized-commercial-enzyme-cocktails
#3
Tiziano Benocci, Paul Daly, Maria Victoria Aguilar Pontes, Kathleen Lail, Mei Wang, Anna Lipzen, Vivian Ng, Igor V Grigoriev, Ronald P de Vries
As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications. We investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS). Initially, CS and SBH resulted in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set was induced, especially for SBH...
September 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30221828/combining-butyrated-mannac-with-glycoengineered-cho-cells-improves-epo-glycan-quality-and-production
#4
Qiong Wang, Cheng-Yu Chung, Weiming Yang, Ganglong Yang, Sandra Chough, Yiqun Chen, Bojiao Yin, Rahul Bhattacharya, Yingwei Hu, Christopher T Saeui, Kevin J Yarema, Michael J Betenbaugh, Hui Zhang
Sodium butyrate (NaBu) is well-known for enhancing protein production, but also degrades glycan quality. In this study, butyrate supplied by the precursor molecule 1,3,4-O-Bu3 ManNAc is applied to overcome the negative effects of NaBu on glycan quality while simultaneously increasing the productivity of the model recombinant erythropoietin (EPO). The beneficial impact of 1,3,4-O-Bu3 ManNAc on EPO glycan quality, while evident in wild-type CHO cells, was particularly pronounced in glycoengineered CHO cells with stable H Supplementation of 1,3,4-O-Bu3 ManNAc achieved approximately 30% sialylation enhancement on EPO protein in wild-type CHO cells...
September 17, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30207077/commentary-continuous-virus-inactivation-how-to-generate-a-plug-flow
#5
Alois Jungbauer
To realize continuous integrated manufacturing of biopharmaceuticals, all steps of production have to be operated in a continuous mode. Virus inactivation is a crucial step in this process, and due to standard procedures and a fixed residence time, it is particularly challenging to realize. Two articles in this issue present possible solutions to this problem.
September 11, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30198629/development-of-capacitance-tools-at-line-method-for-assessing-biomass-of-mammalian-cell-culture-and-fixed-cell-calibration-standard
#6
Juhi Fernandes, Jayme Currie, Kevin Ramer, An Zhang
Capacitance has been used to monitor "biomass" in cell culture and fermentation processes for several decades. The present work explored various ways to support GMP use and create robust methods that would assure proper performance of capacitance equipment. The first goal was to develop a method for measuring at-line capacitance that could serve to correct or confirm online measurements from bioreactors. With appropriate control of culture temperature, vessel geometry, mixing, and timing; an at-line system was developed that consistently predicted online capacitance outputs...
September 10, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30192065/chemical-probes-for-human-udp-glucuronosyltransferases-a-comprehensive-review
#7
REVIEW
Xia Lv, Jian-Bin Zhang, Jie Hou, Tong-Yi Dou, Guang-Bo Ge, Wen-Zhong Hu, Ling Yang
UGTs play crucial roles in the metabolism and detoxification of both endogenous and xenobiotic compounds. The key roles of UGTs in human health have garnered great interest in the design and development of specific probes for human UGTs. However, in contrast to other human enzymes, the probe substrates for human UGTs are rarely reported, owing to the highly overlapping substrate specificities of UGTs and the lack of the integrated crystal structures of UGTs. Over the past decades, many efforts have been made to develop specific probe substrates for UGTs and use them in both basic research and drug discovery...
September 7, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30192055/bioinspired-multi-activities-4d-printing-objects-a-new-approach-toward-complex-tissue-engineering
#8
Chloé D Devillard, Céline A Mandon, Simon A Lambert, Loïc J Blum, Christophe A Marquette
4D printing is an innovative approach which might in a near future lead to the achievement of highly complex smart materials. We are describing here a new strategy for the achievement of 4D printed objects with multiple biological activities. These activities are generate through the entrapment, during 3D printing, of two distinct enzymes (alkaline phosphatase and thrombin). These two enzymes give then the ability to the 4D printed object to generate bioactivities useful for in vitro tissue engineering. Indeed, it was shown that the entrapped alkaline phosphatase enabled the localized and pre-programmed calcification of some 3D object parts while the diffusion of thrombin from the object permitted the formation of fibrin biofilm (including living cells) directly at the surface of 3D object...
September 7, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30179307/autotransporter-mediated-display-of-a-na%C3%A3-ve-affibody-library-on-the-outer-membrane-of-e-coli
#9
Ken G Andersson, Jonas Persson, Stefan Ståhl, John Löfblom
Development of new affinity proteins using combinatorial protein engineering is today established for generation of monoclonal antibodies and also essential for discovery of binders that are based on non-immunoglobulin proteins. Phage display is most frequently used, but yeast display is becoming increasingly popular, partly due to the option of utilizing fluorescence-activated cell sorting (FACS) for isolation of new candidates. Escherichia coli has several valuable properties for library applications and in particular the high transformation efficiency...
September 4, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30179303/pyruvate-kinase-muscle-1-expression-appears-to-drive-lactogenic-behavior-in-cho-cell-lines-triggering-lower-viability-and-productivity-a-case-study
#10
Danming Tang, Jayashree Subramanian, Benjamin Haley, Jordan Baker, Lucas Luo, Wendy Hsu, Peter Liu, Wendy Sandoval, Michael W Laird, Brad Snedecor, Masaru Shiratori, Shahram Misaghi
CHO cell lines are used to express a variety of therapeutic proteins. However, lactogenic behavior displayed by some CHO cell lines during manufacturing processes may result in a decline in viability, productivity, and possible alterations in product quality. In cultured cells, lactate is produced during glycolysis through irreversible conversion of phosphoenolpyruvate to pyruvate and then lactate via sequential function of pyruvate kinase and lactate dehydrogenase (LDH) enzymes. In the process of cell line development (CLD), we identified two lactogenic cell lines expressing different antibody molecules...
September 4, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30171750/pentose-metabolism-in-saccharomyces-cerevisiae-the-need-to-engineer-global-regulatory-systems
#11
REVIEW
Venkatesh Endalur Gopinarayanan, Nikhil U Nair
Extending the host substrate range of industrially relevant microbes, such as Saccharomyces cerevisiae, has been a highly-active area of research since the conception of metabolic engineering. Yet, rational strategies that enable non-native substrate utilization in this yeast without the need for combinatorial and/or evolutionary techniques are underdeveloped. In this review, we focus on pentose metabolism in S. cerevisiae as a case study to highlight the challenges in this field. In the last three decades, work has focused on expressing exogenous pentose metabolizing enzymes as well as other endogenous enzymes for effective pentose assimilation, growth, and biofuel production...
September 1, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30171736/a-suicide-switch-directly-eliminates-intracellular-scfv-oligomers-in-the-cytoplasm-of-mammalian-cells
#12
Thuy Duong Nguyen, Teruyuki Nagamune, Masahiro Kawahara
As intracellular antibodies (intrabodies) are highly promising tools for drug discovery, we previously developed an innovative antibody screening platform in mammalian cells by a single-chain Fv (scFv)-c-kit growth sensor, which successfully selected rabies nucleoprotein and phosphoprotein-specific intrabodies from a synthetic scFv library. Since the scFv-c-kit growth sensor releases a growth signal after forming oligomers due to binding to an oligomeric antigen, it is critical to use a library which does not contain self-oligomeric scFvs to avoid the off-target signal of the growth sensor...
September 1, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30171735/identifying-stable-fragments-of-arabidopsis-thaliana-cellulose-synthase-subunit-3-by-yeast-display
#13
Maryam Raeeszadeh-Sarmazdeh, Nikhil Patel, Sarah Cruise, Leila Owen, Hugh O'Neill, Eric T Boder
Determining structures of large, complex proteins remains challenging, especially for transmembrane proteins, as the protein size increases. Arabidopsis thaliana cellulose synthesis complex is a large, multimeric complex located in the plant cell membrane that synthesizes cellulose microfibrils in the plant cell wall. Despite the biological and economic importance of cellulose and therefore cellulose synthesis, many aspects of the CSC structure and function are still unknown. Here, we used yeast surface display (YSD) to determine the full-length expression of A...
September 1, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30168658/a-self-assembling-two-dimensional-protein-array-is-a-versatile-platform-for-the-assembly-of-multicomponent-nanostructures
#14
Alexander Thomas, James F Matthaei, François Baneyx
Rationally designed two-dimensional (2D) arrays that support the assembly of nanoscale components are of interest for catalysis, sensing, and biomedical applications. Previously, we reported on the computational redesign of a protein called TTM that undergoes calcium-induced self-assembly into nanostructured lattices capable of growing to dozens of micrometers. We demonstrate here that the N- and C-termini of the constituent monomers are solvent-accessible and that they can be modified with a hexahistidine extension, a gold-binding peptide, or a biotinylation tag to decorate nickel-nitriloacetic acid beads with self-assembled protein islands, conjugate gold nanoparticles to planar arrays, or control the immobilization density of avidin molecules onto 2D lattices through co-polymerization of biotinylated and wild type TTM monomers...
August 31, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30156038/genome-editing-with-crispr-cas9-in-lactobacillus-plantarum-revealed-that-editing-outcomes-can-vary-across-strains-and-between-methods
#15
Ryan T Leenay, Justin M Vento, Malay Shah, Maria Elena Martino, François Leulier, Chase L Beisel
Lactic-acid bacteria such as Lactobacillus plantarum are commonly used for fermenting foods and as probiotics, where increasingly sophisticated genome-editing tools are employed to elucidate and enhance these microbes' beneficial properties. The most advanced tools to-date utilize an oligonucleotide or double-stranded DNA donor for recombineering and Cas9 for target DNA cleavage. As the associated methods are often developed in isolation for one strain, it remains unclear how different Cas9-based editing methods compare across strains...
August 29, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30155990/long-term-retinal-differentiation-of-human-induced-pluripotent-stem-cells-in-a-continuously-perfused-microfluidic-culture-device
#16
Nima Abdolvand, Rui Tostoes, William Raimes, Vijay Kumar, Nicolas Szita, Farlan Veraitch
BACKGROUND: Understanding how microenvironmental cues influence cellular behaviour will enable development of efficient and robust pluripotent stem cell differentiation protocols. Unlike traditional cell culture dishes, microfluidic bioreactors can provide stable microenvironmental conditions by continuous medium perfusion at a controlled rate. The aim of this study was to investigate whether a microfluidic culture device could be used as a perfused platform for long-term cell culture processes such as the retinal differentiation of human induced pluripotent stem cells...
August 29, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30152598/liquid-marbles-as-an-easy-to-handle-compartment-for-cell-free-synthesis-and-in-situ-immobilization-of-recombinant-proteins
#17
Noriho Kamiya, Yuki Ohama, Kosuke Minamihata, Rie Wakabayashi, Masahiro Goto
Liquid marble (LM), a self-standing micro-scale aqueous droplet, has emerged as a micro-bioreactor in biological applications. Herein, we explored the potential of LM as media for cell-free synthesis and simultaneous immobilization of recombinant proteins. Initially, formation of hydrogel marble (HM) by using an enzymatic disulfide-based hydrogelation technique was confirmed by incorporating three components, horseradish peroxidase (HRP), a tetra-thiolated poly(ethylene glycol) derivative and glycyl-L-tyrosine, in LM...
August 28, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30144348/comprehensive-improvement-of-sample-preparation-methodologies-facilitates-dynamic-metabolomics-of-aspergillus-niger
#18
Xiaomei Zheng, Jiandong Yu, Timothy C Cairns, Lihui Zhang, Zhidan Zhang, Qiongqiong Zhang, Ping Zheng, Jibin Sun, Yanhe Ma
Metabolomics is an essential discipline in industrial biotechnology. Sample preparation approaches dramatically influence data quality and, ultimately, interpretation and conclusions from metabolomic experiments. However, standardized protocols for highly reproducible metabolic datasets are limited, especially for the fungal cell factory Aspergillus niger. Here, we developed an improved liquid chromatography-tandem mass spectrometry-based pipeline for A. niger metabolomics. It was found that fast filtration with liquid nitrogen was more suitable for cell quenching, causing minimal disruption to cell integrity, and improved intracellular metabolite recovery when compared to cold methanol quenching approaches...
August 25, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30144330/tailoring-collagen-to-engineer-the-cellular-microenvironment
#19
Richard A Que, Dale R Crakes, Faten Abdulhadi, Chun-Hao Niu, Nancy A Da Silva, Szu-Wen Wang
Collagen is the most abundant protein in the extracellular matrix, and it directs the behavior of the neighboring cells. By customizing properties of collagen, it is possible to control the cells that interact with it. Utilizing a bottom-up strategy, modular gene fragments were assembled and recombinantly processed to create collagen-mimetic variants that modulate proteolytic degradation, cell adhesion, and mechanical characteristics. The removal of the native MMP cleavage site resulted in MMP-1 resistant collagen...
August 25, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/30117715/high-performance-biosensing-systems-based-on-various-nanomaterials-as-signal-transducers
#20
REVIEW
Jaewook Lee, Oluwasesan Adegoke, Enoch Y Park
Recently, highly sensitive and selective biosensors have become necessary for improving public health and well-being. To fulfill this need, high-performance biosensing systems based on various nanomaterials, such as nanoparticles, carbon nanomaterials, and hybrid nanomaterials, are developed. Numerous nanomaterials show excellent physical properties, including plasmonic, magnetic, catalytic, mechanical and fluorescence properties and high electrical conductivities, and these unique and beneficial properties have contributed to the fabrication of high-performance biosensors with various applications, including in optical, electrical, and electrochemical detection platforms...
August 17, 2018: Biotechnology Journal
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