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Cancer Biomarkers: Section A of Disease Markers

Pavel Majek, Klara Pecankova, Jaroslav Cermak, Zdenka Gasova, Bohumir Prochazka, Jan E Dyr
BACKGOUND: It has been indicated in plasma proteomic studies on different myelodysplastic syndrome (MDS) cohorts that alpha-2-HS-glycoprotein could be a promising MDS biomarker candidate. OBJECTIVE: The goal of this work was to estimate alpha-2-HS-glycoprotein (AHSG) plasma levels and its biomarker value in the low- and high-risk subgroups of MDS patients. METHODS: The level of AHSG was estimated for 115 plasma samples using ELISA. RESULTS: The AHSG plasma level was found to be decreased significantly (p= 2...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
Li-Wei Liu, Ming-Ya Yang, Min Zhou, Jia-Jia Li, Bo Liu, Yue-Yin Pan
OBJECTIVE: To investigate the improvement of cytotoxicity of autologous CIKs from patients with breast cancer to MCF-7 cells by suppressed PD-1 expression. METHODS: The Lentiviral Vector/PD-1 carrying the gene that can suppressed PD-1 was transferred to CIK cells from patients with breast cancer to inhibit PD-1 expression. The PD-1 protein were detected by RT-PCR and Western blot. The positive PD-1 of CIKs and PD-L1 of MCF-7 cells were detected by FCM, and cytotoxicity of CIKs to MCF-7 was assayed by CCK-8...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
Lei Jiang, Wen-Jun Wang, Zhan-Wu Li, Xiao-Zhou Wang
BACKGROUND: Gastric cancer is one of the most common malignancies worldwide. Recent studies reported that Piwil3 was overexpressed in various cancers, including gastric cancer (GC). This study was intended to investigate its function and mechanism in GC progress. METHODS: Quantitative real time PCR(RT-PCR) and western blotting assays were utilized to measure mRNA and protein expression levels, respectively. SiRNA transfection was performed to suppress the expression of Piwil3...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
Jing Chen, Shun-Wang Cao, Zhen Cai, Lei Zheng, Qian Wang
BACKGROUND: Epithelial-mesenchymal transition (EMT) plays a crucial role in circulating tumor cells (CTCs) dissemination and cancer metastasis. OBJECTIVE: To investigate the EMT phenotypes of CTCs in hepatocellular carcinoma (HCC) patients and the clinical utility in the early diagnosis of HCC metastasis and progression. METHODS: We retrospectively analyzed the count and EMT classification of CTCs detected by the CanPatrol® platform in 195 HCC patients...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
Ping Zhao, Meng Meng, Bin Xu, Aiping Dong, Guangzhen Ni, Lianfang Lu
MUC1, a membrane tethered mucin glycoprotein, is overexpressed in > 60% of human pancreatic cancers (PCs), and is associated with poor prognosis and enhanced metastasis. Here, we report the effect of silencing MUC1 expression on the growth, migration and invasive ability of pancreatic cancer cells, and explored its mechanisms. We observed that siRNA mediated suppression of the MUC1 expression significantly reduced invasive and migrative capability and induced apoptosis of the pancreatic cancer PANC-1 cells...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
G Q Yuan, N L Wei, X Q Wang, Y N Zhang, L Y Mu, W N Zhou, Y W Pan
BACKGROUND: Although O(6)-methylguanine DNA methyltransferase (MGMT) promoter methylation status is an important marker for glioblastoma multiforme (GBM), there is considerable variability in the clinical outcome of patients with similar methylation profles. OBJECTIVE: We examined whether a MicroRNA (miRNA) signature can be identified for predicting clinical outcomes and helping in treatment decisions. METHODS: The differentially expressed miRNAs were evaluated in 6 pairs of short- (⩽ 450 days) and long-term survivors (> 450 days) by using microarray...
August 25, 2017: Cancer Biomarkers: Section A of Disease Markers
Hiroomi Ogawa, Kyoichi Kaira, Kengo Takahashi, Akira Shimizu, Bolag Altan, Daisuke Yoshinari, Takayuki Asao, Tetsunari Oyama
PURPOSE: The glucose-regulated protein 78 (GRP78), also referred to as immunoglobulin heavy chain binding protein (BiP) (BiP/GRP78), is a major molecular chaperone in the endoplasmic reticulum (ER) and is extensively expressed in human neoplasms. Although the enhanced expression of BiP/GRP78 has been described to be associated with poor prognosis in gastric cancer (GC), details regarding its prognostic significance remain unclear. The aim of this study was to elucidate the prognostic role of BiP/GRP78 in patients with GC...
August 11, 2017: Cancer Biomarkers: Section A of Disease Markers
Wen-Jing Chen, Jing-Ying Ye, Xin Li, Jia Xu, Hai-Jin Yi
OBJECTIVE: This study aims to discuss clinical characteristics, image manifestation and treatment methods of temporal bone lesions with facial paralysis as the main manifestation for deepening the understanding of such type of lesions and reducing erroneous and missed diagnosis. METHODS: The clinical data of 16 patients with temporal bone lesions and facial paralysis as main manifestation, who were diagnosed and treated from 2009 to 2016, were retrospectively analyzed...
August 11, 2017: Cancer Biomarkers: Section A of Disease Markers
Xiaohui Hou, Rui Liu, Canhua Huang, Lu Jiang, Yu Zhou, Qianming Chen
Oral submucous fibrosis (OSF) is a chronic insidious disease which predisposes to oral cancer. Understanding the molecular markers for OSF is critical for diagnosis and treatment of oral cancer. In this study, the proteins expression profile of OSF tissues was compared to normal mucous tissues by 2 dimensional electrophoresis (2-DE). The 2-DE images were analyzed through cut, spot detection and match analysis using mass spectrometry (MS). Differentially expressed genes were identified as candidates. RT-PCR, Western Blot and immunohistochemistry were performed to validate the difference in expression of the candidates between OSF and normal mucous tissues...
August 11, 2017: Cancer Biomarkers: Section A of Disease Markers
De-Zhi Liu, Hui Zhao, Qin-Guang Zou, Qing-Jie Ma
BACKGROUND: Previous studies indicated that microRNA-338-5p (miR-338-5p) functions as tumor suppressor in some cancer types including glioma. However, the clinical significance and biological function of miR-338-5p in glioma still need to be explored. METHODS: We used quantitative real time PCR (qRT-PCR) to detect the miR-338-5p expression in the 44 cases of glioma tissues and adjacent normal tissues. In vitro, CCK8 cell proliferation, cell colony formation, transwell invasion assay and flow cytometry analysis were performed to explore the effects of miR-338-5p on cell proliferation, cell invasion and cell cycle distribution...
August 11, 2017: Cancer Biomarkers: Section A of Disease Markers
Jing Wang, Xin Xie, Feng Cheng, Xin Zhou, Jun Xia, Xifeng Qian, Lingling Wang, Hongfeng Guo
BACKGROUND: Red blood cell distribution width (RDW) has been reported as an inflammatory biomarker and a predictor of prognosis in different types of cancer. However, the role of RDW at diagnosis in patients with multiple myeloma (MM) has been less explored. OBJECTIVE: We aimed to investigate the association between RDW and the response to treatment and overall survival (OS) in patients with MM. METHODS: We retrospectively analyzed the data for 196 MM patients between January 1, 2007 and December 31, 2015...
August 11, 2017: Cancer Biomarkers: Section A of Disease Markers
Rui Xie, Shang-Nong Wu, Cheng-Cheng Gao, Xiao-Zhong Yang, Hong-Gang Wang, Jia-Ling Zhang, Wei Yan, Tian-Heng Ma
BACKGROUND: microRNA (miR)-1290 was previously indicated to promote esophageal squamous cell carcinoma (ESCC) progression via regulating its target gene nuclear factor I/X (NFIX). OBJECTIVE: To investigate clinical significance of miR-1290 and NFIX in ESCC. METHODS: Quantitative real-time PCR was performed to detect miR-1290 and NFIX mRNA expression in ESCC tissues. Associations of miR-1290 and/or NFIX mRNA expression with various clinicopathological features and prognosis in ESCC patients were statistically evaluated...
August 4, 2017: Cancer Biomarkers: Section A of Disease Markers
Mitra Modarres-Gilani, Maryam Vaezi, Mamak Shariat, Narges Zamani, Roghaiyeh Nourizadeh
BACKGROUND: Lifetime risk of developing endometrial cancer is 2.17%. There is controversy regarding the predictive value of Cancer Antigen 125 (CA125) in endometrial cancer as well as the significance of its relationship with prognostic factors and appropriate cut-off value. OBJECTIVE: The aim of the present study was to investigate the prognostic role of CA125 in advanced endometrial carcinoma and determination of the appropriate cut-off value. METHODS: A sample of 91 patients was retrospectively selected from a population of 501 patients suffering from endometrial cancer during 1995 to 2015 in accordance with the inclusion criteria...
August 4, 2017: Cancer Biomarkers: Section A of Disease Markers
Yi-Sheng Wei, Ya-Guang Zhou, Guo-Ying Wang, Zhi-Hua Liang, Min-Rui Luo, Tian-Ai Yang, Jun Huang
BACKGROUND AND OBJECTIVE: The association of chemotherapy-associated hemoglobin and survival of colorectal cancer (CRC) receiving adjuvant chemotherapy is uncertain. We sought to explore the prognostic value of chemotherapy-associated hemoglobin in CRC receiving adjuvant chemotherapy and the best cut point affecting prognosis. METHODS: Three hundred and twenty stage II and III CRC patients receiving adjuvant FOLFOX chemotherapy from March 2003 to March 2012 were enrolled...
August 1, 2017: Cancer Biomarkers: Section A of Disease Markers
Guang-Wei Liu, Zhao-Min Qin, Qin-Hai Shen
OBJECTIVE: It is crucially important to discover the relationships between genes and microRNAs (miRNAs) in cancer. Thus, we proposed a combined bioinformatics method integrating Pearson's correlation coefficient (PCC), Lasso, and causal inference method (IDA) to identify the potential miRNA targets for stomach adenocarcinoma (STAD) using Borda count election. MATERIALS AND METHODS: Firstly, the ensemble method integrating PCC, IDA, and Lasso was used to predict miRNA targets...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
Li-Li Mei, Yun-Tan Qiu, Meng-Bing Huang, Wen-Jun Wang, Jie Bai, Zhi-Zhou Shi
miR-99a is down-regulated in esophageal squamous cell carcinoma (ESCC), however the role and underlying mechanism are still unknown. We aim to explore the role and mechanism of miR-99a down-regulation in ESCC. The expression of miR-99a in ESCC tissues and cell lines was detected by Human miRNA Microarrays and Real-time PCR. The effects of miR-99a on cell proliferation, migration and invasion were determined by Cell Counting Kit-8 (CCK-8) assay, transwell migration and invasion assay. Target gene of miR-99a were analyzed by target prediction software and validated by Real-time PCR and Western blotting assay...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
Xun Gou, Xiyan Zhao, Zhengrong Wang
BACKGROUND: Long noncoding RNAs (lncRNA) have been verified to be involved in hepatocellular carcinoma (HCC) progression. However, the potential biologic function of PVT1 in HCC is not still fully known. METHODS: PVT1 and miR-214 were detected by qRT-PCR assays in HCC tissues and adjacent normal tissues. CCK8, cell colony and transwell invasion assays were performed to evaluate cell proliferation and invasion abilities. Western-blot assay was performed to detect the protein of E-cadherin and Vimentin...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
Véronique Quillien, Audrey Lavenu, François Ducray, David Meyronet, Olivier Chinot, Frédéric Fina, Marc Sanson, Catherine Carpentier, Lucie Karayan-Tapon, Pierre Rivet, Natacha Entz-Werle, Michèle Legrain, Emmanuèle Lechapt Zalcman, Guenaelle Levallet, Fabienne Escande, Carole Ramirez, Dan Chiforeanu, Elodie Vauleon, Dominique Figarella-Branger
BACKGROUND: Pyrosequencing is recognized as a strong technique to analyze the MGMT status of glioblastoma patients. The most commonly used assay, quantifies the methylation levels of CpGs 74 to 78. A more recent CE-marked In Vitro Diagnostic Medical Device (CE-IVD) assay, Therascreen, analyzes CpGs 76-79. METHODS: We performed a comparison of these two assays to evaluate the potential impact of this shift in analyzed CpGs. Therascreen analysis was centrally performed for 102 glioblastoma patients, who were part of a prospective multicenter trial...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
Fadi Najjar, Moocheer Alammar, Ghassan Al-Massarani, Nissreen Almalla, Abdulmunim Aljapawe, Adnan Ikhtiar
BACKGROUND: Circulating endothelial cells (CECs) and microparticles (MPs) are proposed as useful biosensors for angiogenesis and membrane damage in cancer. OBJECTIVE: We investigated their predictive value for progression disease (PD) and clinical outcomes in advanced non-small cell lung cancer (NSCLC) patients treated with cytotoxic chemotherapy. METHODS: Peripheral blood samples were obtained from 60 patients. Immunomagnetic separation (IMS) and flow cytometry techniques were used to quantify CECs and MPs, respectively...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
Hongbin Shi, Jianping Yu, Jie Li
BACKGROUND: The nephroblastoma overexpressed gene (NOV) expressions in tissues and organs has become abnormal during tumorigenesis and progression. This study intended to investigate the correlation between clinical outcomes and NOV expression in renal cell carcinoma (RCC) patients. METHODS: Fifty RCC patients who attended the hospital from January 2013 to January 2015 were enrolled in this study. NOV expression in cancerous tissues and adjacent non-cancerous renal tissues of RCC patients were detected by immunohistochemistry (IHC)...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
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