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Diagnostic Pathology

Bin Chang, Jiao Meng, Huimin Zhu, Xiang Du, Lili Sun, Lei Wang, Shugang Li, Gong Yang
BACKGROUND: Regulated in development and DNA damage response (REDD1), a gene responding to hypoxia or multiple DNA damage events, was recently implicated in cancer development and progression. Previously, in vivo and in vitro experiments indicated that REDD1 functions as an oncogene in ovarian cancer cells. However, the role of REDD1 in cancer cell migration and invasion and in clinical significance of prognostic values is not examined in detail. METHODS: We detected the REDD1 protein expression by immunohistochemistry in 18 normal ovarian surface epithelium or fallopian tube epithelium specimens, 24 ovarian borderline tumors, and 229 ovarian cancers...
November 14, 2018: Diagnostic Pathology
Changsong Wang, Tian Yun, Zhicheng Wang, Nianlong Meng, Naijun Fan, Xuexia Lv, Fulin Li
OBJECTIVE: To study the clinicopathological characteristics and genetic features of melanin-producing medullary thyroid carcinoma (MP-MTC). METHODS: The immunophenotype of MP-MTC was studied using the immunohistochemical method, and its genetic features were assayed using an amplification refractory mutation system or PCR method. RESULTS: A 71-year-old man presented with a slowly growing 5-cm mass on the left side of the neck for approximately two months...
November 13, 2018: Diagnostic Pathology
Zhenyu Zhang, Jinfeng Zhu, Yansong Huang, Weibing Li, Hongqiu Cheng
BACKGROUND: MYO18B has been identified as a novel tumor suppressor gene in several cancers. However, its specific roles in the progression of hepatocellular carcinoma (HCC) has not been well defined. METHODS: We firstly identified the expression and prognostic values of MYO18B in HCC using TCGA cohort and our clinical data. Then, MYO18B knockdown by RNA inference was implemented to investigate the effects of MYO18B on HCC cells. Quantitative RT-PCR and Western blot were used to determine gene and protein expression levels...
November 3, 2018: Diagnostic Pathology
Nozomu Kurose, Michiho Takenaka, Manabu Yamashita, Chie Shimaguchi, Mariko Nakano, Bryant Britni, Xin Guo, Chizuru Futatsuya, Akihiro Shioya, Sohsuke Yamada
BACKGROUND: Nephroblastoma (NB) is a malignant embryonal neoplasm derived from nephrogenic blastemal cells. NB usually forms a solid mass, but in extremely rare cases, it may show cystic changes. CASE PRESENTATION: A six-month-old girl with persistent high fevers was found to have pyuria and bacteriuria. Ultrasonography revealed multilocular cysts in the right kidney. Right nephrectomy was performed with cyst wall rupture during surgery. An intraoperative rapid diagnosis, based on peritoneal fluid cytology, confirmed three components of blastemal, stromal, and epithelial cells...
October 27, 2018: Diagnostic Pathology
Kerstin Hartmann, Kornelia Schlombs, Mark Laible, Claudia Gürtler, Marcus Schmidt, Ugur Sahin, Hans-Anton Lehr
BACKGROUND: Tissue heterogeneity in formalin-fixed paraffin-embedded (FFPE) breast cancer specimens may affect the accuracy of reverse transcription quantitative real-time PCR (RT-qPCR). Herein, we tested the impact of tissue heterogeneity of breast cancer specimen on the RT-qPCR-based gene expression assay MammaTyper®. METHODS: MammaTyper® quantifies the mRNA expression of the four biomarkers ERBB2, ESR1, PGR, and MKI67. Based on pre-defined cut-off values, this molecular in vitro diagnostic assay permits binary marker classification and determination of breast cancer subtypes as defined by St Gallen 2013...
October 20, 2018: Diagnostic Pathology
Xingen Wang, Chi-Sing Ng, Cuimin Chen, Guangyin Yu, Weihua Yin
BACKGROUND: Indolent T-cell proliferative disorder of the GIT is a rare and provisional entity in the revised WHO 2016 classification. The patients usually have prolonged survival with persistent disease even without any treatment. CASE PRESENTATION: The 46 years old male patient has been followed up for more than 6 years without chemotherapy. Repeated gastrointestinal biopsies showed expansion of the lamina propria extending to the submucosa by small to medium sized lymphocytes with minimal cytologic atypia...
October 20, 2018: Diagnostic Pathology
Dóra Mihály, Noémi Nagy, Gergő Papp, Zsuzsanna Pápai, Zoltán Sápi
BACKGROUND: Synovial sarcoma is a rare soft tissue tumor which contains the unique SS18-SSX1, SS18-SSX2 - or, rarely, SS18-SSX4 - fusion transcripts. It is well known that some soft tissue tumors, like Ewing sarcomas and myxoid liposarcomas, can spread via the blood with free circulating tumor cells (CTC); this can be detected by several sensitive molecular biology methods. Here we report a study of fifteen synovial sarcoma patients with varied clinical backgrounds. METHOD: After blood withdrawal and nucleic acid isolation, we attempted to detect the SS18-SSX fusion genes from circulating tumor cells or cell-free nucleic acids with nested PCR and droplet digital PCR...
October 17, 2018: Diagnostic Pathology
Weigang Dai, Jinning Ye, Zhimei Zhang, Liang Yang, Hui Ren, Hui Wu, Jianhui Chen, Jieyi Ma, Ertao Zhai, Shirong Cai, Yulong He
BACKGROUND: Heat shock factor 1 (HSF1) was initially identified as a transcription factor encoding heat shock proteins, which assist in refolding or degrading damaged proteins. Recent studies have reported that HSF1 can act as an oncogene that regulates tumour progression. The present study aimed to elucidate the clinicopathological significance and prognostic value of HSF1 expression in gastric cancer (GC). METHODS: The data from The Cancer Genome Atlas (TCGA) were used to analyse HSF1 expression in GC and normal tissues, while 8 pairs of freshly frozen tissue samples were used to investigate HSF1 expression at the mRNA and protein levels in GC tissues and adjacent normal tissues using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting assays...
October 17, 2018: Diagnostic Pathology
Saška Brajkovic, Benjamin Pelz, Maria-Giuseppina Procopio, Anne-Laure Leblond, Grégoire Repond, Ariane Schaub-Clerigué, Diego G Dupouy, Alex Soltermann
BACKGROUND: Anaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing for rapid immunohistochemistry/immunofluorescence (IHC/IF) stainings of formalin-fixed paraffin-embedded (FFPE) or frozen tissue samples. METHODS: A novel ALK IF protocol was developed for the MTP device using the primary mouse anti-human ALK antibody clone 5A4...
October 16, 2018: Diagnostic Pathology
Sarah M Choi, Aleodor A Andea, Min Wang, Amir Behdad, Lina Shao, Yanming Zhang, Xinyan Lu, David Dittmann, Juan Castro, Yi-Hua Chen, Juehua Gao
BACKGROUND: Transformation of follicular lymphoma most typically occurs as diffuse large B-cell lymphoma, however other forms of transformation such as classic Hodgkin lymphoma and lymphoblastic transformation can occur. Secondary malignant histiocytosis also represents a rare form of transformation, which is thought to occur due to a process of transdifferentiation whereby the lymphoma cells exhibit lineage plasticity and lose all evidence of B-cell phenotype and instead acquire the phenotype of a histiocytic neoplasm...
October 15, 2018: Diagnostic Pathology
J Kevin Hicks, Evita Henderson-Jackson, Julia Duggan, David M Joyce, Andrew S Brohl
BACKGROUND: RAF family activating fusions have been described as a potentially targetable molecular finding in a subset of soft tissue sarcomas. To further expand upon the landscape of this genetic feature, we describe a novel MTAP-RAF1 activating fusion identified in a S100 positive soft tissue sarcoma. CASE PRESENTATION: A 51 year old man underwent excision of a soft tissue mass in his foot. Pathology revealed a spindle cell neoplasm with S100 positivity, ultimately classified as a soft tissue sarcoma, not otherwise specified...
October 12, 2018: Diagnostic Pathology
André Homeyer, Seddik Hammad, Lars Ole Schwen, Uta Dahmen, Henning Höfener, Yan Gao, Steven Dooley, Andrea Schenk
BACKGROUND: Automated image analysis enables quantitative measurement of steatosis in histological images. However, spatial heterogeneity of steatosis can make quantitative steatosis scores unreliable. To improve the reliability, we have developed novel scores that are "focused" on steatotic tissue areas. METHODS: Focused scores use concepts of tile-based hotspot analysis in order to compute statistics about steatotic tissue areas in an objective way. We evaluated focused scores on three data sets of images of rodent liver sections exhibiting different amounts of dietary-induced steatosis...
September 20, 2018: Diagnostic Pathology
Andréanne Gagné, Bernard Têtu, Michèle Orain, Stéphane Turcotte, Marie Plante, Jean Grégoire, Marie-Claude Renaud, Isabelle Bairati, Dominique Trudel
It has been highlighted that the original article [1] contained a typesetting mistake in the family name of Dominique Trudel.
September 17, 2018: Diagnostic Pathology
Keiko Segawa, Shintaro Sugita, Tomoyuki Aoyama, Terufumi Kubo, Hiroko Asanuma, Taro Sugawara, Yumika Ito, Mitsuhiro Tsujiwaki, Hiromi Fujita, Makoto Emori, Tadashi Hasegawa
BACKGROUND: Clear cell sarcoma of soft tissue (CCSST) and clear cell sarcoma-like gastrointestinal tumor (CCSLGT) are malignant mesenchymal tumors that share some pathological features, but they also have several different characteristics. They are well known to express chimeric fusions of Ewing sarcoma breakpoint region 1 (EWSR1) and cAMP response element-binding protein (CREB) family members; namely, EWSR1-activating transcription factor 1 (ATF1) and EWSR1-CREB1. In addition, recent studies have suggested the presence of other fusions...
September 15, 2018: Diagnostic Pathology
Katherine Wickenden, Nadia Nawaz, Sami Mamand, Deevia Kotecha, Amy L Wilson, Simon D Wagner, Matthew J Ahearne
BACKGROUND: Abnormally sustained immune reactions drive B-cell proliferation in some cases of marginal zone lymphoma but the CD4+ T-cell subsets, which are likely to contribute to the B-cell responses in the tumour microenvironment, are not well characterised and neither has the spatial distribution of the different subsets in involved lymph nodes been investigated. METHODS: Employing a workflow of multiplex semi-automated immunohistochemistry combined with image processing we investigated association between infiltrating T-cells and proliferating lymphoma B-cells...
September 15, 2018: Diagnostic Pathology
Lemeng Sun, Liangshu Feng, Jiuwei Cui
BACKGROUND: Hepatocellular carcinoma (HCC) is the second leading cause of cancer death in Asia; however, the molecular mechanism in its tumorigenesis remains unclear. Abnormal expression of claudins (CLDNs), a family of tight junction (TJ) proteins, plays an important role in the metastatic phenotype of epithelial-derived tumors by affecting tight junction structure, function and related cellular signaling pathways. In a previous study, we used a tissue chip assay to identify CLDN17 as an upregulated gene in HCC...
September 15, 2018: Diagnostic Pathology
Jian Tan, Jianpeng Hu, Yonghui He, Feilun Cui
This article [1] is retracted at request of the Editor. After publication of this article [1] concerns were raised regarding aspects of the methodology, including the choice of stains being inappropriate or inadequate to answer the research question. A discrepancy was also noted between the number of groups described in the methods and the number of groups presented in some of the figures. Furthermore the western blot images did not entirely correspond with the information reported in the results. For these reasons the conclusions of the study cannot be considered to be supported by the data...
September 12, 2018: Diagnostic Pathology
Xinyu Yang, Haichao Wang, Menmen Zhang, Jin Liu, Ben Lv, Fangping Chen
This article [1] is retracted at request of the Editor.
September 12, 2018: Diagnostic Pathology
Meifeng Zhou, Xiuzhen Tong
This article [1] is retracted at request of the Editor.
September 12, 2018: Diagnostic Pathology
Mark Kriegsmann, Katharina Kriegsmann, Alexander Harms, Rémi Longuespée, Christiane Zgorzelski, Jonas Leichsenring, Thomas Muley, Hauke Winter, Daniel Kazdal, Benjamin Goeppert, Arne Warth
BACKGROUND: Non-small cell lung cancer (NSCLC) and melanoma are frequent entities in routine diagnostics. Whereas the differential diagnosis is usually straight forward based on histomorphology, it can be challenging in poorly differentiated tumors as melanoma may mimic various histological patterns. Distinction of the two entities is of outmost importance as both are treated differently. HMB45 and MelanA are recommended immunohistological markers for melanoma in this scenario. SOX10 has been described as an additional marker for melanoma...
September 11, 2018: Diagnostic Pathology
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