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Journal of Integrative Plant Biology

Jiawei Pan, Dahui Huang, Zhonglong Guo, Zheng Kuang, He Zhang, Xinyu Xie, Zengfeng Ma, Shaopei Gao, Manuel T Lerdau, Chengcai Chu, Lei Li
The ability of a plant to produce grain, fruit, or forage depends ultimately on photosynthesis. There have been few attempts, however, to study microRNAs, which are a class of endogenous small RNAs post-transcriptionally programming gene expression, in relation to photosynthetic traits. We focused on miR408, one of the most conserved plant miRNAs, and overexpressed it in parallel in Arabidopsis, tobacco, and rice. The transgenic plants all exhibited increased copper content in the chloroplast, elevated abundance of plastocyanin, and an induction of photosynthetic genes...
January 13, 2018: Journal of Integrative Plant Biology
Chengkai Lu, Jinfeng Qi, Christian Hettenhausen, Yunting Lei, Jingxiong Zhang, Mou Zhang, Cuiping Zhang, Juan Song, Jing Li, Guoyan Cao, Saif Ul Malook, Jianqiang Wu
Atmospheric CO2 levels are rapidly increasing due to human activities. However, the effects of elevated CO2 (ECO2 ) on plant defense against insects and the underlying mechanisms remain poorly understood. Here we show that ECO2 increased the photosynthetic rates and the biomass of tobacco and rice plants, and the chewing lepidopteran insects Spodoptera litura and Mythimna separata gained less and more mass on tobacco and rice plants, respectively. Consistently, under ECO2 , the levels of jasmonic acid (JA), the main phytohormone controlling plant defense against these lepidopteran insects, as well as the main defense-related metabolites, were increased and decreased in insect-damaged tobacco and rice plants...
January 10, 2018: Journal of Integrative Plant Biology
Lei He, Sen Zhang, Zhennan Qiu, Juan Zhao, Wendan Nie, Haiyan Lin, Zhengge Zhu, Dali Zeng, Qian Qian, Li Zhu
Chloroplast genes are transcribed by the PEP or NEP. FRUCTOKINASE-LIKE PROTEINS (FLNs) are phosphofructokinase-B (PfkB)-type carbohydrate kinases that act as part of the PEP complex; however, the molecular mechanisms underlying FLNs activity in rice remain elusive. Previously, we identified and characterized a heat-stress sensitive albino (hsa1) mutant in rice. Map-based cloning revealed that HSA1 encodes a putative OsFLN2. Here, we further demonstrated that knockdown or knockout of the OsFLN1, a close homolog of HSA1/OsFLN2, considerably inhibits chloroplast biogenesis and the fln1 knockout mutants created by CRISPR/Cas9 exhibit severe albino phenotype and seedling lethality...
January 10, 2018: Journal of Integrative Plant Biology
Minliang Jin, Xiangguo Liu, Wei Jia, Haijun Liu, Wenqiang Li, Yong Peng, Yanfang Du, Yuebin Wang, Yuejia Yin, Xuehai Zhang, Qing Liu, Min Deng, Nan Li, Xiyan Cui, Dongyun Hao, Jianbing Yan
Flowering time is a trait vital to the adaptation of flowering plants to different environments. Here, we report that CCT domain genes play an important role in flowering in maize (Zea mays L.). Among the 53 CCT family genes we identified in maize, 28 were located in flowering time quantitative trait locus regions and 15 were significantly associated with flowering time based on candidate-gene association mapping analysis. Furthermore, a CCT gene named ZmCOL3 was shown to be a repressor of flowering. Overexpressing ZmCOL3 delayed flowering time by approximately 4 days, in either long-day or short-day conditions...
January 10, 2018: Journal of Integrative Plant Biology
Jin-Xing Zhou, Zhang-Wei Liu, Yong-Qiang Li, Lin Li, Bangjun Wang, She Chen, Xin-Jian He
LHP1 mediates recruitment of the PRC2 histone methyltransferase complex to chromatin and thereby facilitates maintenance of H3K27me3 on FLC, a key flowering repressor gene. Here, we found that the PWWP domain proteins (PDPs) interact with FVE and MSI5 to suppress FLC expression and thereby promote flowering. We demonstrated that FVE, MSI5, and PDP3 were co-purified with LHP1. The H3K27me3 level on FLC was decreased in the pdp mutants as well as in the fve/msi5 double mutant. This study suggests that PDPs function together with FVE and MSI5 to regulate the function of the PRC2 complex on FLC...
January 4, 2018: Journal of Integrative Plant Biology
Qian Qian
Rice is one of the most important staple cereals, feeds over 60% of China's population and contributes nearly 40% of the nation's total calorie intake (Chen et al. 2007). A continuous increase in rice production is required to meet the demands of increasing population and food consumption (Tilman et al. 2011).
January 4, 2018: Journal of Integrative Plant Biology
Muhammad Abid, Zhongwei Tian, Jinling Hu, Attiq Ullah, Yakun Cui, Shafaqat Ali, Suyu Jiang, Rizwan Zahoor, Yonghui Fan, Jiang Dong, Tingbo Dai
Drought stress during grain filling of wheat alter enzyme activities, which may cause a reduction in carbohydrate translocation from the source and poor accumulation in the developing grains. This study investigated whether pre-anthesis drought priming improves grain filling response to post-anthesis drought stress in wheat by regulating the activities of carbohydrate-metabolizing enzymes in source and sink organs of wheat. The plants of a drought-sensitive and -tolerant wheat cultivar were exposed to moderate drought stress during tillering for priming, and a subsequent severe drought stress was applied at seven days after anthesis...
December 20, 2017: Journal of Integrative Plant Biology
Bing Wang, Jinping Wei, Na Song, Ning Wang, Zhensheng Kang
NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30, from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one-hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C-terminus is necessary for the activation of transcription...
December 18, 2017: Journal of Integrative Plant Biology
Joao Benhur Mokochinski, Paulo Mazzafera, Alexandra Christine Helena Frankland Sawaya, Roland Mumm, Ric Cornelis Hendricus de Vos, Robert David Hall
Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may affect the metabolism of three species of Eucalyptus, E. dunnii, E. grandis and E. pellita. Young plants were grown for 53 days in the greenhouse and then transferred to growth chambers at 10, 20 or 30°C for another 7 days...
December 16, 2017: Journal of Integrative Plant Biology
Wai Li Lim, Helen M Collins, Rohan R Singh, Natalie Aj Kibble, Kuok Yap, Jillian Taylor, Geoffrey B Fincher, Rachel A Burton
Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however recalcitrance of hull-less cultivars to transformation has limited use of these varieties. To overcome this, we have sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over-express genes encoding synthases for the important dietary fibre component, (1,3;1,4)-β-glucan (mixed-linkage glucan), primarily found in starchy endosperm cell walls...
December 16, 2017: Journal of Integrative Plant Biology
Di Wu, Yanan Liu, Fan Xu, Yuelin Zhang
BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) plays critical roles in plant developmental and immune signaling pathways. BAK1 and a large number of LRR-RLKs harbor a mysterious carboxyl terminal tail (CT) beyond their kinase domain. In this study we analyzed the biological significance of this CT region using a unique bak1 mutant allele which causes deletion of the CT region. We showed that BAK1 CT promotes its kinase activity and is required for PAMP-triggered immunity, but it is dispensable for brassinosteroid responses and BAK1/BKK1-inhibited cell death signaling...
December 11, 2017: Journal of Integrative Plant Biology
Zhiming Yu, Qiyuan Chen, Weiwei Chen, Xian Zhang, Fengling Mei, Pengcheng Zhang, Mei Zhao, Xiaohong Wang, Nongnong Shi, Stephen Jackson, Yiguo Hong
We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms...
December 11, 2017: Journal of Integrative Plant Biology
Xiaoliang Shi, Sunlu Chen, Yu Peng, Yufeng Wang, Jiugeng Chen, Zhanghua Hu, Baohe Wang, Aihong Li, Daiyin Chao, Yuhong Li, Sheng Teng
Since its domestication from wild rice thousands of years ago, rice has been cultivated largely through transplantation. During transplantation from the nursery to the paddy field, rice seedlings experience transplantation shock which affects their physiology and production. However, the mechanisms underlying transplantation shock and rice adaptation to this shock are largely unknown. Here, we isolated a transplant-sensitive chloroplast-deficient (tsc1) rice mutant that produces albino leaves after transplantation...
December 6, 2017: Journal of Integrative Plant Biology
Jinshan Zhang, Hui Zhang, José Ramón Botella, Jian-Kang Zhu
In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used CRISPR/Cas9 to introduce a loss-of-function mutation into the Waxy gene in two widely-cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties...
December 6, 2017: Journal of Integrative Plant Biology
Wei Xiong, Tiantian Ye, Xuan Yao, Xiong Liu, Sheng Ma, Xi Chen, Ming-Luan Chen, Yu-Qi Feng, Yan Wu
The phytohormones gibberellic acid (GA) and abscisic acid (ABA) antagonistically control seed germination. High levels of GA favor seed germination, whereas high levels of ABA hinder this process. The direct relationship between GA biosynthesis and seed germination ability need further investigation. Here, we identified the ABA-insensitive gain-of-function mutant germination insensitive to ABA mutant 2 (gim2) by screening a population of XVE T-DNA-tagged mutant lines. Based on two loss-of-function gim2-ko mutant lines, the disruption of GIM2 function caused a delay in seed germination...
December 4, 2017: Journal of Integrative Plant Biology
Erbao Zhan, Huapeng Zhou, Sha Li, Lei Liu, Tinghong Tan, Honghui Lin
The conjugation of SUMO (small ubiquitin-like modifier) to protein substrates is a reversible process (SUMOylation/deSUMOylation) that regulates plant development and stress responses. The essential metal copper (Cu) is required for normal plant growth, but excess amounts are toxic. The SUMO E3 ligase SIZ1 and SIZ1-mediated SUMOylation function in plant tolerance to excess Cu. It is unknown whether deSUMOylation also contributes to Cu tolerance in plants. Here we report that OTS1, a protease that cleaves SUMO from its substrate proteins, participates in Cu tolerance in Arabidopsis thaliana (Arabidopsis)...
December 4, 2017: Journal of Integrative Plant Biology
Sangseok Lee, Lidiya I Sergeeva, Dick Vreugdenhil
Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies were done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a UPLC triple quadrupole mass spectrometer. Root system architecture (RSA) of the set of accessions was quantified, using a new parameter (mature root unit) for complex root systems, and correlated with the phytohormone data...
December 2, 2017: Journal of Integrative Plant Biology
Madhvi Soni, Kalyan K Mondal
Xanthomonas axonopodis pv. punicae (Xap) causing bacterial blight is an important pathogen that incurs significant losses to the exportability of pomegranate. Xap employs Xop TTSS-effector via type three secretion system to suppress pomegranate immunity. Here, we investigate the role of XopL during blight pathogenesis. We found that XopL is essential for its in planta growth and full virulence. Leaves inoculated with Xap ΔxopL produced restricted water-soaked lesions compared to that inoculated with Xap wild...
November 29, 2017: Journal of Integrative Plant Biology
Fei Xie, Hailong Yan, Yang Sun, Yameng Wang, Hong Chen, Wanying Mao, Liyao Zhang, Mengxiang Sun, Xiongbo Peng
During embryogenesis, plants are thought to utilize a mechanism that allows the suspensor to maintain its identity. Here, we reported that RPL18aB was involved in this mechanism in Arabidopsis thaliana. The suspensor cells proliferated in rpl18aB and formed a multicellular structure rather than underwent programmed cell death as in wild type. Suspensors of rpl18aB expressed the embryo proper marker DRN::GFP but not the suspensor marker WOX8::GFP. In addition, auxin accumulated throughout the suspensors of rpl18aB proembryos...
November 29, 2017: Journal of Integrative Plant Biology
Xiao-Bo Zhang, Bao-Hua Feng, Hui-Mei Wang, Xia Xu, Yong-Feng Shi, Yan He, Zheng Chen, Atul Prakash Sathe, Lei Shi, Jian-Li Wu
We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed splHM47 ). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating Phe to Ile at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype...
November 29, 2017: Journal of Integrative Plant Biology
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