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Journal of Integrative Plant Biology

Zhongfeng Li, Lingxue Jiang, Yansong Ma, Zhongyan Wei, Huilong Hong, Zhangxiong Liu, Jinhui Lei, Ying Liu, Rongxia Guan, Yong Guo, Longguo Jin, Lijuan Zhang, Yinghui Li, Yulong Ren, Wei He, Ming Liu, Nang Myint Phyu Sin Htwe, Lin Liu, Bingfu Guo, Jian Song, Bing Tan, Guifeng Liu, Maiquan Li, Xianli Zhang, Bo Liu, Xuehui Shi, Sining Han, Sunan Hua, Fulai Zhou, Lili Yu, Yanfei Li, Shuang Wang, Jun Wang, Ruzhen Chang, Lijuan Qiu
Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS) -induced soybean (Glycine max) population, consisting of 21,600 independent M2 lines, was developed. Over one thousand M4 (5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology, and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development...
October 24, 2016: Journal of Integrative Plant Biology
Shuangshuang Zhao, Lei Zhao, Fengxia Liu, Yongzhen Wu, Zuofeng Zhu, Chuanqing Sun, Lubin Tan
Grain yield in rice (Oryza sativa L.) is closely related to leaf and flower development. Coordinative regulation of leaf, pollen, and seed development in rice as a critical biological and agricultural question should to be addressed. Here we identified two allelic rice mutants with narrow and semi-rolled leaves, named narrow and rolled leaf 2-1 (nrl2-1) and nrl2-2. Map-based molecular cloning revealed that NRL2 encodes a novel protein with unknown biochemical function. The mutation of NRL2 caused pleiotropic effects, including a reduction in the number of longitudinal veins, defective abaxial sclerenchymatous cell differentiation, abnormal tapetum degeneration and microspore development, and the formation of more slender seeds compared with the wild type (WT)...
October 20, 2016: Journal of Integrative Plant Biology
Mei Chen, Jin Zhang, Lei Zhao, Jiale Xing, Lianwei Peng, Tingyun Kuang, Jean-David Rochaix, Fang Huang
We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2 ) for 25 days with more than 30-fold yield increase compared to wild type. Thus hpm91 displays a higher capacity of H2 production than a previously characterised pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2 production is due to enhanced stability of PSII which correlates with increased ROS scavenging capacity during sulfur deprivation...
October 20, 2016: Journal of Integrative Plant Biology
Bin Cai, Xiangxiong Kong, Chao Zhong, Suli Sun, Xiao Feng Zhou, Yin Hua Jin, Youning Wang, Xia Li, Zhendong Zhu, Jing Bo Jin
SIZ1 is a small ubiquitin-related modifier (SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However, the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max (soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid (SA), heat, and dehydration treatment, but not in response to cold, ABA, and NaCl treatment...
October 20, 2016: Journal of Integrative Plant Biology
Lan Shen, Chun Wang, Yaping Fu, Junjie Wang, Qing Liu, Xiaoming Zhang, Changjie Yan, Qian Qian, Kejian Wang
Grain yield is one of the most important and complex trait for genetic improvement in crops; it is known to be controlled by a number of genes known as quantitative trait loci (QTLs). In the past decade, many yield-contributing QTLs have been identified in crops. However, it remains unclear whether those QTLs confer the same yield performance in different genetic backgrounds. Here, we performed CRISPR/Cas9-mediated QTL editing in five widely-cultivated rice varieties and revealed that the same QTL can have diverse, even opposing, effects on grain yield in different genetic backgrounds...
September 15, 2016: Journal of Integrative Plant Biology
Xiaoba Wu, Jinxin Liu, Dongqi Li, Chun-Ming Liu
The rice endosperm plays crucial roles in nourishing the embryo during embryogenesis and seed germination. Although previous studies have provided the general information about rice endosperm, a systematic investigation throughout the entire endosperm developmental process is still lacking. In this study, we examined in detail rice endosperm development on a daily basis throughout the 30-day period of post-fertilization development. We observed that coenocytic nuclear division occurred in the first 2 days after pollination (DAP), cellularization occurred between 3 and 5 DAP, differentiation of the aleurone and starchy endosperm occurred between 6 and 9 DAP, and accumulation of storage products occurred concurrently with the aleurone/starchy endosperm differentiation from 6 DAP onwards and was accomplished by 21 DAP...
September 2016: Journal of Integrative Plant Biology
Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, YaoYao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang, Sanwen Huang
We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polymorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homology to Arabidopsis SEPALLATA2 (SEP2) thus being designated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity...
September 2016: Journal of Integrative Plant Biology
Linda Brochhausen, Jan Maisch, Peter Nick
Nuclear migration and positioning are crucial for the morphogenesis of plant cells. We addressed the potential role of nuclear positioning for polarity induction using an experimental system based on regenerating protoplasts, where the induction of a cell axis de novo can be followed by quantification of specific regeneration stages. Using overexpression of fluorescently tagged extranuclear (perinuclear actin basket, kinesins with a calponin homology domain (KCH)) as well as intranuclear (histone H2B) factors of nuclear positioning and time-lapse series of the early stages of regeneration, we found that nuclear position is no prerequisite for polarity formation...
September 2016: Journal of Integrative Plant Biology
Gaojie Ji, Jie Zhang, Haiying Zhang, Honghe Sun, Guoyi Gong, Jianting Shi, Shouwei Tian, Shaogui Guo, Yi Ren, Huolin Shen, Junping Gao, Yong Xu
Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4), expressed specifically in carpel primordia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism (SNPs) and one InDel identified in the coding region of CitACS4, the C364W mutation located in the conserved box 6 was co-segregated with andromonoecy...
September 2016: Journal of Integrative Plant Biology
Xiaoba Wu, Jinxin Liu, Dongqi Li, Chun-Ming Liu
Rice caryopsis as one of the most important food sources for humans has a complex structure that is composed of maternal tissues including the pericarp and testa and filial tissues including the endosperm and embryo. Although rice caryopsis studies have been conducted previously, a systematic characterization throughout the entire developmental process is still lacking. In this study, detailed morphological examinations of caryopses were made during the entire 30-day developmental process. We observed some rapid changes in cell differentiation events and cataloged how cellular degeneration processes occurred in maternal tissues...
September 2016: Journal of Integrative Plant Biology
Hyeran Kim, Sang-Tae Kim, Jahee Ryu, Min Kyung Choi, Jiyeon Kweon, Beum-Chang Kang, Hyo-Min Ahn, Suji Bae, Jungeun Kim, Jin-Soo Kim, Sang-Gyu Kim
CRISPR-Cas9 system is now widely used to edit a target genome in animals and plants. Cas9 protein derived from Streptococcus pyogenes (SpCas9) cleaves double-stranded DNA targeted by a chimeric single-guide RNA (sgRNA). For plant genome editing, Agrobacterium-mediated T-DNA transformation has been broadly used to express Cas9 proteins and sgRNAs under the control of CaMV 35S and U6/U3 promoter, respectively. We here developed a simple and high-throughput binary vector system to clone a 19-20 bp of sgRNA, which binds to the reverse complement of a target locus, in a large T-DNA binary vector containing an SpCas9 expressing cassette...
August 2016: Journal of Integrative Plant Biology
Dongdong Xu, Hailong Yang, Cheng Zou, Wen-Xue Li, Yunbi Xu, Chuanxiao Xie
Eukaryotic Argonaute proteins play primary roles in miRNA and siRNA pathways that are essential for numerous developmental and biological processes. However, the functional roles of the four ZmAGO1 genes have not yet been characterized in maize (Zea mays L.). In the present study, ZmAGO1a was identified from four putative ZmAGO1 genes for further characterization. Complementation of the Arabidopsis ago1-27 mutant with ZmAGO1a indicated that constitutive overexpression of ZmAGO1a could restore the smaller rosette, serrated leaves, later flowering and maturation, lower seed set, and darker green leaves at late stages of the mutant to the wild-type phenotype...
August 2016: Journal of Integrative Plant Biology
Yuan Li, Ying Chang, Chongchong Zhao, Hailian Yang, Dongtao Ren
Leaf senescence is the final leaf developmental process that is regulated by both intracellular factors and environmental conditions. The mitogen-activated protein kinase (MAPK) signaling cascades have been shown to play important roles in regulating leaf senescence; however, the component(s) downstream of the MAPK cascades in regulating leaf senescence are not fully understood. Here we showed that the transcriptions of ZmMEK1, ZmSIMK1, and ZmMPK3 were induced during dark-induced maize leaf senescence. Furthermore, in-gel kinase analysis revealed the 42 kDa MAPK was activated...
August 2016: Journal of Integrative Plant Biology
Yugo Lima-Melo, Fabricio E L Carvalho, Márcio O Martins, Gisele Passaia, Rachel H V Sousa, Milton C Lima Neto, Márcia Margis-Pinheiro, Joaquim A G Silveira
The physiological role of plant mitochondrial glutathione peroxidases is scarcely known. This study attempted to elucidate the role of a rice mitochondrial isoform (GPX1) in photosynthesis under normal growth and salinity conditions. GPX1 knockdown rice lines (GPX1s) were tested in absence and presence of 100 mM NaCl for 6 d. Growth reduction of GPX1s line under non-stressful conditions, compared with non-transformed (NT) plants occurred in parallel to increased H2 O2 and decreased GSH contents. These changes occurred concurrently with photosynthesis impairment, particularly in Calvin cycle's reactions, since photochemical efficiency did not change...
August 2016: Journal of Integrative Plant Biology
Lichao Zhang, Guoxiang Liu, Jizeng Jia, Guangyao Zhao, Chuan Xia, Lina Zhang, Fu Li, Qiang Zhang, Chunhao Dong, Shuangcheng Gao, Longzhi Han, Xiuping Guo, Xin Zhang, Jinxia Wu, Xu Liu, Xiuying Kong
Through large-scale transformation analyses, TaMYB72 was identified as a flowering time regulator in wheat. TaMYB72 is a MYB family transcription factor localized to the nucleus. Three TaMYB72 homologs, TaMYB72-A, TaMYB72-B and TaMYB72-D, cloned from hexaploid wheat were mapped to the short arm of the group 6 chromosomes. Under the long-day conditions, over-expression of the TaMYB72 in rice shortened the flowering time by approximately 12 d. Expression analyses suggest that TaMYB72 may function through up-regulation of florigen genes Hd3a and RFT1...
August 2016: Journal of Integrative Plant Biology
Shuang-Quan Huang, Xiang-Ping Wang, Shi-Guo Sun
The evolution of long corolla tubes has been hypothesized to be driven by long-tongued pollinators. Corolla tubes in Pedicularis species can be longer than 10 cm which may function as flower stalks to increase visual attractiveness to pollinators because these species provide no nectar and are pollinated by bumblebees. The corolla tube length was manipulated (shorter or longer) in two Pedicularis species in field to examine whether longer tubes are more attractive to pollinators and produce more seeds than short tubes...
August 2016: Journal of Integrative Plant Biology
Yingni Xiao, Shawn Thatcher, Min Wang, Tingting Wang, Mary Beatty, Gina Zastrow-Hayes, Lin Li, Jiansheng Li, Bailin Li, Xiaohong Yang
Starch is the major component in maize kernels, providing a stable carbohydrate source for humans and livestock as well as raw material for the biofuel industry. Increasing maize kernel starch content will help meet industry demands and has the potential to increase overall yields. We developed a pair of maize near-isogenic lines (NILs) with different alleles for a starch quantitative trait locus on chromosome 3 (qHS3), resulting in different kernel starch content. To investigate the candidate genes for qHS3 and elucidate their effects on starch metabolism, RNA-Seq was performed for the developing kernels of the NILs at 14 and 21 d after pollination (DAP)...
August 2016: Journal of Integrative Plant Biology
Huihui Liu, Chuanwei Yang, Lin Li
A better understanding of shade avoidance syndrome (SAS) is an urgent need because of its effect on energy reallocation. Leverage-related mechanism in crops is of potential economic interest for agricultural applications. Here we report the SAS phenotype at tissue level rice seedlings. Tissue-specific RNA-sequencing indicates auxin plays different roles between coleoptile and the first leaf. Phenotypes of wild type treated by gibberellin and brassinosteroid biosynthesis inhibitors and of related mutants suggest these two hormones positively regulate SAS...
July 2016: Journal of Integrative Plant Biology
Xiao-Ying Dou, Ke-Zhen Yang, Zhao-Xia Ma, Li-Qun Chen, Xue-Qin Zhang, Jin-Rong Bai, De Ye
In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the process, the molecular mechanisms of pollen tube growth remains poorly understood. In this study, we identified that the Arabidopsis Transmembrane Protein 18 (AtTMEM18) gene played important roles in pollen tube growth. The AtTMEM18 shares a high similarity with the Transmembrane 18 proteins (TMEM18s) that are conserved in most eukaryotes and may play important roles in obesity in humans...
July 2016: Journal of Integrative Plant Biology
Jasmin Herden, Stefan Meldau, Sang-Gyu Kim, Grit Kunert, Youngsung Joo, Ian T Baldwin, Meredith C Schuman
Arabidopsis thaliana plants are less resistant to attack by the generalist lepidopteran herbivore Trichoplusia ni when plants and herbivores are entrained to opposite, versus identical diurnal cycles and tested under constant conditions. This effect is associated with circadian fluctuations in levels of jasmonic acid, the transcription factor MYC2, and glucosinolate contents in leaves. We tested whether a similar effect could be observed in a different plant-herbivore system: the wild tobacco Nicotiana attenuata and its co-evolved specialist herbivore, Manduca sexta...
July 2016: Journal of Integrative Plant Biology
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