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Purinergic Signalling

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https://www.readbyqxmd.com/read/29236227/loss-of-vascular-expression-of-nucleoside-triphosphate-diphosphohydrolase-1-cd39-in-hypertension
#1
Charlotte Roy, Julie Tabiasco, Antoine Caillon, Yves Delneste, Jean Merot, Julie Favre, Anne Laure Guihot, Ludovic Martin, Daniele C Nascimento, Bernhard Ryffel, Simon C Robson, Jean Sévigny, Daniel Henrion, Gilles Kauffenstein
Ectonucleoside triphosphate diphosphohydrolase-1, the major vascular/immune ectonucleotidase, exerts anti-thrombotic and immunomodulatory actions by hydrolyzing extracellular nucleotides (danger signals). Hypertension is characterized by vascular wall remodeling, endothelial dysfunction, and immune infiltration. Here our aim was to investigate the impact of arterial hypertension on CD39 expression and activity in mice. Arterial expression of CD39 was determined by reverse transcription quantitative real-time PCR in experimental models of hypertension, including angiotensin II (AngII)-treated mice (1 mg/kg/day, 21 days), deoxycorticosterone acetate-salt mice (1% salt and uninephrectomy, 21 days), and spontaneously hypertensive rats...
December 13, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29188550/udp-induced-relaxation-is-enhanced-in-aorta-from-female-obese-otsuka-long-evans-tokushima-fatty-rats
#2
Shota Kobayashi, Takayuki Matsumoto, Makoto Ando, Maika Iguchi, Shun Watanabe, Kumiko Taguchi, Tsuneo Kobayashi
Uridine 5'-diphosphate (UDP) plays an important role in controlling vascular tone; however, UDP-mediated response in metabolic syndromes, including obesity and type 2 diabetes in females, remains unclear. In this study, we investigated UDP-mediated response in the aorta of female obese Otsuka Long-Evans Tokushima Fatty (OLETF) rats and control Long-Evans Tokushima Otsuka (LETO) rats. In OLETF rat aortas precontracted by phenylephrine (PE) (vs. LETO), (1) UDP-induced relaxation was increased, whereas acetylcholine (ACh)-induced relaxation was decreased; (2) no UDP- or ACh-induced relaxations were observed in endothelial denudation, whereas UDP-induced small contraction was observed; and (3) NG-nitro-L-arginine [L-NNA, a nitric oxide (NO) synthase inhibitor] eliminated UDP-induced relaxation and small contraction, whereas caused contrasting responses by ACh, including slight relaxations (LETO) and contractions (OLETF)...
November 29, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29170977/species-differences-and-mechanism-of-action-of-a3-adenosine-receptor-allosteric-modulators
#3
Lili Du, Zhan-Guo Gao, Silvia Paoletta, Tina C Wan, Elizabeth T Gizewski, Samantha Barbour, Jacobus P D van Veldhoven, Adriaan P IJzerman, Kenneth A Jacobson, John A Auchampach
Activity of the A3 adenosine receptor (AR) allosteric modulators LUF6000 (2-cyclohexyl-N-(3,4-dichlorophenyl)-1H-imidazo [4,5-c]quinolin-4-amine) and LUF6096 (N-{2-[(3,4-dichlorophenyl)amino]quinolin-4-yl}cyclohexanecarbox-amide) was compared at four A3AR species homologs used in preclinical drug development. In guanosine 5'-[γ-[(35)S]thio]triphosphate ([(35)S]GTPγS) binding assays with cell membranes isolated from human embryonic kidney cells stably expressing recombinant A3ARs, both modulators substantially enhanced agonist efficacy at human, dog, and rabbit A3ARs but provided only weak activity at mouse A3ARs...
November 23, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29164451/the-potential-of-p2x7-receptors-as-a-therapeutic-target-including-inflammation-and-tumour-progression
#4
REVIEW
Geoffrey Burnstock, Gillian E Knight
Seven P2X ion channel nucleotide receptor subtypes have been cloned and characterised. P2X7 receptors (P2X7R) are unusual in that there are extra amino acids in the intracellular C terminus. Low concentrations of ATP open cation channels sometimes leading to cell proliferation, whereas high concentrations of ATP open large pores that release inflammatory cytokines and can lead to apoptotic cell death. Since many diseases involve inflammation and immune responses, and the P2X7R regulates inflammation, there has been recent interest in the pathophysiological roles of P2X7R and the potential of P2X7R antagonists to treat a variety of diseases...
November 21, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29159762/p2y12-receptor-upregulation-in-satellite-glial-cells-is-involved-in-neuropathic-pain-induced-by-hiv-glycoprotein-120-and-2-3-dideoxycytidine
#5
Zhihua Yi, Lihui Xie, Congfa Zhou, Huilong Yuan, Shuai Ouyang, Zhi Fang, Shanhong Zhao, Tianyu Jia, Lifang Zou, Shouyu Wang, Yun Xue, Bing Wu, Yun Gao, Guilin Li, Shuangmei Liu, Hong Xu, Changshui Xu, Chunping Zhang, Shangdong Liang
The direct neurotoxicity of HIV and neurotoxicity of combination antiretroviral therapy medications both contribute to the development of neuropathic pain. Activation of satellite glial cells (SGCs) in the dorsal root ganglia (DRG) plays a crucial role in mechanical and thermal hyperalgesia. The P2Y12 receptor expressed in SGCs of the DRG is involved in pain transmission. In this study, we explored the role of the P2Y12 receptor in neuropathic pain induced by HIV envelope glycoprotein 120 (gp120) combined with ddC (2',3'-dideoxycytidine)...
November 20, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29134411/distinct-roles-of-ecto-nucleoside-triphosphate-diphosphohydrolase-2-ntpdase2-in-liver-regeneration-and-fibrosis
#6
Linda Feldbrügge, Z Gordon Jiang, Eva Csizmadia, Shuji Mitsuhashi, Stephanie Tran, Eric U Yee, Sonja Rothweiler, Kahini A Vaid, Jean Sévigny, Moritz Schmelzle, Yury V Popov, Simon C Robson
Ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases) are cell surface-located transmembrane ecto-enzymes of the CD39 superfamily which regulate inflammation and tissue repair by catalyzing the phosphohydrolysis of extracellular nucleotides and modulating purinergic signaling. In the liver, NTPDase2 is reportedly expressed on portal fibroblasts, but its functional role in regulating tissue regeneration and fibrosis is incompletely understood. Here, we studied the role of NTPDase2 in several models of liver injury using global knockout mice...
November 13, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29116551/anti-inflammatory-effect-of-a-novel-locally-acting-a2a-receptor-agonist-in-a-rat-model-of-oxazolone-induced-colitis
#7
L Antonioli, A El-Tayeb, C Pellegrini, M Fornai, O Awwad, G Giustarini, G Natale, L Ryskalin, Z H Németh, C E Müller, C Blandizzi, R Colucci
Adenosine represents a powerful modulating factor, which has been shown to orchestrate the scope, duration, and remission of the inflammatory response through the activation of four specific receptors, classified as A1, A2A, A2B, and A3, all being widely expressed in a variety of immune cells. Several selective A2A receptor agonists have displayed anti-inflammatory effects, through the suppression of IL-12, TNF, and IFN-γ production by monocytes and lymphocytes, in the setting of chronic intestinal inflammation...
November 8, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29086245/myeloid-p2y2-receptor-promotes-acute-inflammation-but-is-dispensable-for-chronic-high-fat-diet-induced-metabolic-dysfunction
#8
Samantha E Adamson, Garren Montgomery, Scott A Seaman, Shayn M Peirce-Cottler, Norbert Leitinger
The purinergic receptor P2Y2 binds ATP to control chemotaxis of myeloid cells, and global P2Y2 receptor knockout mice are protected in models of acute inflammation. Chronic inflammation mediated by macrophages and other immune cells in adipose tissue contributes to the development of insulin resistance. Here, we investigate whether mice lacking P2Y2 receptors on myeloid cells are protected against acute and chronic inflammation. Wild-type mice were transplanted with either wild-type or P2Y2 receptor null bone marrow and treated with a sublethal dose of endotoxin as a model of acute inflammation, or fed a high-fat diet to induce obesity and insulin resistance as a model of chronic inflammation...
October 30, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/29022161/pom-1-inhibits-p2-receptors-and-exhibits-anti-inflammatory-effects-in-macrophages
#9
Gabriela Pimenta-Dos-Reis, Eduardo José Lopes Torres, Paula Gabriela Quintana, Lincon Onorio Vidal, Bárbara Andréa Fortes Dos Santos, Chuan-Sheng Lin, Norton Heise, Pedro Muanis Persechini, Julieta Schachter
Extracellular nucleotides can modulate the immunological response by activating purinergic receptors (P2Rs) on the cell surface of macrophages, dendritic, and other immune cells. In particular, the activation of P2X7R can induce release of cytokines and cell death as well as the uptake of large molecules through the cell membrane by a mechanism still poorly understood. Polyoxotungstate-1 (POM-1) has been proposed as a potent inhibitor of ecto-nucleotidases, enzymes that hydrolyze extracellular nucleotides, regulating the activity of P2Rs...
October 11, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28956227/various-n-glycoforms-differentially-upregulate-e-ntpdase-activity-of-the-ntpdase3-cd39l3-ecto-enzymatic-domain
#10
Alexander H Zhong, Z Gordon Jiang, Richard D Cummings, Simon C Robson
The GDA1/CD39 ecto-nucleoside triphosphate diphosphosphohydrolase (E-NTPDase) superfamily is a group of eight heavily glycosylated ecto-enzymes that hydrolyze extracellular nucleosides di- and tri-phosphates in the presence of divalent cations, to generate the monophosphate derivatives. This catalytic process differentially regulates a complex array of purinergic signaling responses. NTPDase3/CD39L3is dominantly expressed in pancreatic islet cells, where it may regulate insulin secretion, and has seven N-linked glycosylation sites with four close to five highly conserved domains called "apyrase conserved regions" (ACRs)...
September 27, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28929376/divergent-coronary-flow-responses-to-uridine-adenosine-tetraphosphate-in-atherosclerotic-apoe-knockout-mice
#11
Bunyen Teng, Hicham Labazi, Changyan Sun, Yan Yang, Xiaorong Zeng, S Jamal Mustafa, Zhichao Zhou
Uridine adenosine tetraphosphate (Up4A) exerts potent relaxation in porcine coronary arteries that is reduced following myocardial infarction, suggesting a crucial role for Up4A in the regulation of coronary flow (CF) in cardiovascular disorders. We evaluated the vasoactive effects of Up4A on CF in atherosclerosis using ApoE knockout (KO) mice ex vivo and in vivo. Functional studies were conducted in isolated mouse hearts using the Langendorff technique. Immunofluorescence was performed to assess purinergic P2X1 receptor (P2X1R) expression in isolated mouse coronary arteries...
September 20, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28905300/p2x7r-antagonism-after-subfailure-overstretch-injury-of-blood-vessels-reverses-vasomotor-dysfunction-and-prevents-apoptosis
#12
Weifeng Luo, Daniel Feldman, Reid McCallister, Colleen Brophy, Joyce Cheung-Flynn
Human saphenous vein (HSV) is harvested and prepared prior to implantation as an arterial bypass graft. Injury and the response to injury from surgical harvest and preparation trigger cascades of molecular events and contribute to graft remodeling and intimal hyperplasia. Apoptosis is an early response after implantation that contributes the development of neointimal lesions. Here, we showed that surgical harvest and preparation of HSV leads to vasomotor dysfunction, increased apoptosis and downregulation of the phosphorylation of the anti-apoptotic protein, Niban...
September 13, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28895091/abstracts-from-the-2017-italian-german-purine-club-meeting
#13
(no author information available yet)
No abstract text is available yet for this article.
September 11, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28879644/adenosine-reduces-reactive-oxygen-species-and-interleukin-8-production-by-trichomonas-vaginalis-stimulated-neutrophils
#14
Amanda Piccoli Frasson, Camila Braz Menezes, Gustavo Krumel Goelzer, Simone Cristina Baggio Gnoatto, Solange Cristina Garcia, Tiana Tasca
Trichomonas vaginalis is a flagellated protozoan that affects the human urogenital tract causing 276.4 million new infections a year. The parasite elicits a vaginal mucosal infiltration of immune cells, especially neutrophils which are considered to be primarily responsible for cytological change observed at the infection site as well as the major contributor in the inflammatory response against the parasite. Extracellular nucleotides and their nucleosides are signaling compounds involved in several biological processes, including inflammation and immune responses...
September 6, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28875249/from-the-7th-joint-italian-german-purine-club-meeting-to-european-purine-club-meetings
#15
EDITORIAL
Felicita Pedata
No abstract text is available yet for this article.
September 5, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28840511/nanoparticle-encapsulated-emodin-decreases-diabetic-neuropathic-pain-probably-via-a-mechanism-involving-p2x3-receptor-in-the-dorsal-root-ganglia
#16
Lin Li, Xuan Sheng, Shanhong Zhao, Lifang Zou, Xinyao Han, Yingxin Gong, Huilong Yuan, Liran Shi, Lili Guo, Tianyu Jia, Shuangmei Liu, Bing Wu, Zhihua Yi, Hui Liu, Yun Gao, Guilin Li, Guodong Li, Chunping Zhang, Hong Xu, Shangdong Liang
Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes mellitus (DM). More than 90% of all cases of DM belong to type 2 diabetes mellitus (T2DM). Emodin is the main active component of Radix et rhizoma rhei and has anti-bacterial, anti-viral, anti-ulcerogenic, anti-inflammatory, and anti-cancer effects. Nanoparticle encapsulation of drugs is beneficial for drug targeting and bioavailability as well as for lowering drug toxicity side effects. The aim of this study was to investigate the effects of nanoparticle-encapsulated emodin (nano emodin) on diabetic neuropathic pain (DNP) mediated by the Purin 2X3 (P2X3) receptor in the dorsal root ganglia (DRG)...
August 24, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28828576/bone-turnover-is-altered-in-transgenic-rats-overexpressing-the-p2y2-purinergic-receptor
#17
Maria Ellegaard, Cansu Agca, Solveig Petersen, Ankita Agrawal, Lars Schack Kruse, Ning Wang, Alison Gartland, Jens-Erik Beck Jensen, Niklas Rye Jørgensen, Yuksel Agca
It is now widely recognized that purinergic signaling plays an important role in the regulation of bone remodeling. One receptor subtype, which has been suggested to be involved in this regulation, is the P2Y2 receptor (P2Y2R). In the present study, we investigated the effect of P2Y2R overexpression on bone status and bone cell function using a transgenic rat. Three-month-old female transgenic Sprague Dawley rats overexpressing P2Y2R (P2Y2R-Tg) showed higher bone strength of the femoral neck. Histomorphometry showed increase in resorptive surfaces and reduction in mineralizing surfaces...
August 21, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28823092/inhibition-of-p2x7-receptors-improves-outcomes-after-traumatic-brain-injury-in-rats
#18
Xiaofeng Liu, Zhengqing Zhao, Ruihua Ji, Jiao Zhu, Qian-Qian Sui, Gillian E Knight, Geoffrey Burnstock, Cheng He, Hongbin Yuan, Zhenghua Xiang
Traumatic brain injury (TBI) is the leading cause of death and disability for people under the age of 45 years worldwide. Neuropathology after TBI is the result of both the immediate impact injury and secondary injury mechanisms. Secondary injury is the result of cascade events, including glutamate excitotoxicity, calcium overloading, free radical generation, and neuroinflammation, ultimately leading to brain cell death. In this study, the P2X7 receptor (P2X7R) was detected predominately in microglia of the cerebral cortex and was up-regulated on microglial cells after TBI...
August 19, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28822016/in-situ-imaging-reveals-properties-of-purinergic-signalling-in-trigeminal-sensory-ganglia-in-vitro
#19
Arletta Nowodworska, Arn M J M van den Maagdenberg, Andrea Nistri, Elsa Fabbretti
Chronic pain is supported by sterile inflammation that induces sensitisation of sensory neurons to ambient stimuli including extracellular ATP acting on purinergic P2X receptors. The development of in vitro methods for drug screening would be useful to investigate cell crosstalk and plasticity mechanisms occurring during neuronal sensitisation and sterile neuroinflammation. Thus, we studied, at single-cell level, membrane pore dilation based on the uptake of a fluorescent probe following sustained ATP-gated P2X receptor function in neurons and non-neuronal cells of trigeminal ganglion cultures from wild-type (WT) and R192Q CaV2...
August 18, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28815408/diphenyl-diselenide-modulates-nucleotidases-reducing-inflammatory-responses-in-the-liver-of-toxoplasma-gondii-infected-mice
#20
Pedro H Doleski, Daniela B R Leal, Vanessa S Machado, Nathieli B Bottari, Alessandra G Manzoni, Emerson A Casali, Cesar E J Moritz, Ana C A Rocha, Giovana Camillo, Fernanda F Vogel, Lenita M Stefani, Ricardo E Mendes, Aleksandro Schafer da Silva
The aim of this study was to verify the effect of diphenyl diselenide (PhSe)2 on hepatic nucleotidases and on the concentration of purines in mice infected by Toxoplasma gondii. The animals were divided into four groups: Group A (uninfected), Group B (uninfected and treated with (PhSe)2), Group C (infected), and Group D (infected and treated with (PhSe)2). The inoculation (groups C and D) was performed with 50 cysts of T. gondii (ME-49 strain). Mice from groups B and D were treated with 5 μmol kg(-1) of (PhSe)2...
August 16, 2017: Purinergic Signalling
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