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BMC Veterinary Research

Nienke Endenburg, Sirikul Soontararak, Chalermpol Charoensuk, Hein A van Lith
BACKGROUND: This study investigated whether the body condition score (BCS) and/or culture influences the quality of life (QoL) of dogs, as evaluated by the owner, and whether the BCS is influenced by feeding and exercise and its owner's culture. To this end, a questionnaire was administered to 355 selected dog owners (Thai and Dutch). Their dogs had a BCS of 3 (normal weight), 4 (overweight) or 5 (obese) but no other physical problems. Instead of using Likert scales, continuous scales were used...
July 9, 2018: BMC Veterinary Research
N Wagmann, C Spadavecchia, U Morath-Huss, G Schüpbach-Regula, P Zanolari
BACKGROUND: Certified Swiss farmers are allowed to disbud their goat kids using a standard intramuscularly administered anaesthetic mixture. This mixture, containing xylazine and ketamine, is officially distributed with the goal to provide a painless disbudding. This study aimed to evaluate the quality of analgesia and anaesthesia achieved during disbudding, when performed by Swiss farmers. To assess this, 174 goat kids at 31 different farms were observed and filmed during cautery disbudding...
July 9, 2018: BMC Veterinary Research
Jorge Orós, María López-Yánez, Francisco Rodríguez, Pascual Calabuig, Pedro L Castro
BACKGROUND: Cytokeratins with epitopes in common with those of alpha (acidic and basic) mammalian keratins have been immunohistochemically demonstrated in the epidermis of reptiles. However, there are no reports of immunohistochemical staining patterns of alpha-keratins in other tissues from reptiles. Because the epithelial tumours usually retain the keratin patterns of their normal epithelial origin, it is necessary to know in advance these patterns in the major normal epithelia and organs...
July 6, 2018: BMC Veterinary Research
Nika Brkljača Bottegaro, Jelena Gotić, Jelena Šuran, Diana Brozić, Karla Klobučar, Krunoslav Bojanić, Zoran Vrbanac
BACKGROUND: Oxidative stress (OS) associated with an intense exercise may have a negative influence on equine health. The aim of this study was to determine the effects of endurance races on oxidative and antioxidative status of horses by evaluating changes in reactive oxygen metabolites (d-ROMs), malondialdehyde (MDA), biological antioxidant potential (BAP) and oxidative stress index (OSI) values. The study was carried out on 53 race starts (28 individual horses) competing at different endurance races according to distance (40 and 80 km) and difficulty (easy and demanding)...
July 6, 2018: BMC Veterinary Research
Tadesse Eguale
BACKGROUND: Poultry is one of the common sources of non-typhoidal Salmonella and poultry products are the major sources of human infection with non-typhoidal Salmonella. In spite of flourishing poultry industry in the country, data on prevalence and antimicrobial susceptibility of non-typhoidal Salmonella serovars at farm level is not available in Ethiopia. This study investigated prevalence, serotype distribution and antimicrobial resistance of non-typhoidal Salmonella in poultry farms in Addis Ababa and its surrounding districts...
July 6, 2018: BMC Veterinary Research
Cun Liu, Yanhan Liu, Ye Tian, Xuehua Wei, Yue Zhang, Fulin Tian
BACKGROUND: Aujeszky's disease virus (ADV) can cause neurologic disease in young pigs, respiratory disease in older pigs and abortion or birth of mummified fetuses or stillborn neonates. The re-emergence of Aujeszky's disease (AD) in pig farms vaccinated with live vaccine (Bartha-K61) caused substantial economic losses to Chinese pig industry since late 2011. A field ADV, named Qihe547, was isolated from pigs that exhibited suspected AD clinical symptoms. To better understand the genetic characteristics and mutations of Qihe547 ADV, the whole genome was sequenced and analyzed...
July 6, 2018: BMC Veterinary Research
Shenglan Hu, Yu Wang, Xiaolu Wen, Li Wang, Zongyong Jiang, Chuntian Zheng
BACKGROUND: Chitosan was used as an alternative to promote the growth of weaned piglets. And low-molecular-weight chitosan (LC) is one of chitosan derivatives and maintain beneficial biological properties of chitoson. The present experiment was carried out to examine the effects of LC on the growth performance, intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets. RESULTS: A total of 40 piglets weaned at 21 d of age, with average body weight 6...
July 4, 2018: BMC Veterinary Research
Paolo Motta, Thibaud Porphyre, Saidou M Hamman, Kenton L Morgan, Victor Ngu Ngwa, Vincent N Tanya, Eran Raizman, Ian G Handel, Barend Mark Bronsvoort
BACKGROUND: In sub-Saharan Africa, livestock transhumance represents a key adaptation strategy to environmental variability. In this context, seasonal livestock transhumance also plays an important role in driving the dynamics of multiple livestock infectious diseases. In Cameroon, cattle transhumance is a common practice during the dry season across all the main livestock production zones. Currently, the little recorded information of the migratory routes, grazing locations and nomadic herding practices adopted by pastoralists, limits our understanding of pastoral cattle movements in the country...
July 3, 2018: BMC Veterinary Research
Susanna Pilichi, Stefano Rocca, Maria Dattena, Roy Ransom Pool, Laura Mara, Daniela Sanna, Gerolamo Masala, Maria Lucia Manunta, Simone Dore, Andrea Manunta, Eraldo Sanna Passino
BACKGROUND: Articular cartilage lacks a regenerative response. Embryonic stem cells (ESCs) are a source of pluripotent cells for cartilage regeneration. Their use, however, is associated with a risk of teratoma development, which depends on multiple factors including the number of engrafted cells and their degree of histocompatibility with recipients, the immunosuppression of the host and the site of transplantation. Colonies of sheep embryonic stem-like (ES-like) cells from in vitro-produced embryos, positive for stage-specific embryonic antigens (SSEAs), alkaline phosphatase (ALP), Oct 4, Nanog, Sox 2 and Stat 3 gene expression, and forming embryoid bodies, were pooled in groups of two-three, embedded in fibrin glue and engrafted into osteochondral defects in the left medial femoral condyles of 3 allogeneic ewes (ES)...
June 28, 2018: BMC Veterinary Research
Doris Hoeltig, Florian Nietfeld, Katrin Strutzberg-Minder, Judith Rohde
BACKGROUND: Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia. For control of the disease the detection of sub-clinically infected pigs is of major importance to avoid transmitting of subclinical infections. One method recommended is the testing of tonsillar samples for the presence of A. pleuropneumoniae. This is routinely done by PCR techniques. However, based upon PCR susceptibility testing and monitoring of resistance development is impossible...
June 28, 2018: BMC Veterinary Research
Gian Luca Rovesti, Veronica Devesa, Laura Bertorelli, Jesus Rodriguez-Quiros
BACKGROUND: Stifle arthroscopy has been described to have high sensitivity and specificity in the evaluation of menisci in dogs, particularly for the medial meniscus. However, the visualization of menisci can be difficult. The use of femoral distractors in human medicine has been described to simplify demanding surgical procedures, such as meniscus transplantation. In veterinary medicine, stifle distraction has been reported to facilitate access to the joint and visualization of intra-articular structures, but there are no studies reporting the use of a stifle distraction technique while performing challenging surgical procedures, such as meniscal suture, in clinical patients...
June 28, 2018: BMC Veterinary Research
Ingrid Bortolin Affonso Lux Hoppe, Andréa Souza Ramos de Medeiros, Clarice Weis Arns, Samir Issa Samara
BACKGROUND: The cattle industry is one of the most important Brazilian agribusiness sectors and is a strong contributor to the national economy. Annually about 44.6 million calves are bred, which makes the optimal management of these animals extremely important. Several diseases can affect the initial stages of the bovine production chain, being the bovine respiratory syncytial virus (BRSV) one of the most relevant pathogens. This study aimed to characterize the epidemiology of BRSV infection in dairy cattle herds of São Paulo State, Brazil, using serological and risk factors analyses...
June 27, 2018: BMC Veterinary Research
Han Zhou, Xuechun Li, Zongying Wang, Jiyuan Yin, Hongchao Tan, Li Wang, Xinyuan Qiao, Yanping Jiang, Wen Cui, Min Liu, Yijing Li, Yigang Xu, Lijie Tang
BACKGROUND: Lactobacillus casei (L. casei) is well known for its probiotic property in human and animals. Lactoferricin (Lfcin) polypeptide can effectively modulate host immune responses and have antimicrobial activity in vivo and in vitro. In order to develop a food-grade L. casei system constitutively expressing bovine Lfcin, this study constructed a thymidine auxotrophy (ΔthyA) recombinant L. casei. RESULTS: Based on the thymidylate synthase gene (thyA) insert site, LFEC(Lfcin expression cassette)was inserted into L...
June 27, 2018: BMC Veterinary Research
Karina Retter, Karl-Heinz Esser, Matthias Lüpke, John Hellmann, Dieter Steinhagen, Verena Jung-Schroers
BACKGROUND: Common carp Cyprinus carpio is an important food fish in Central Europe, which in some regions is consumed as part of local tradition. The majority of carp are sold by small retailers and not processed in commercial processing plants. The overall objective of this study was to monitor how animal welfare is safeguarded during the stunning and slaughtering of carp for retail sale. For this, the stunning and slaughtering process was monitored on 12 carp farms. Four welfare-related parameters were assessed: (i) stunning success, (ii) injuries related to the applied stunning method, (iii) time between stunning and slaughter, and (iv) visible responses of carp during slaughtering...
June 27, 2018: BMC Veterinary Research
Rebecca Ricci, Daniele Conficoni, Giada Morelli, Carmen Losasso, Leonardo Alberghini, Valerio Giaccone, Antonia Ricci, Igino Andrighetto
BACKGROUND: Although the European Pet Food Industry Federation (FEDIAF) stated that labels must be accurate and provide detailed information on the ingredients, mislabeling of pet food has been documented by several authors. This phenomenon is of particular concern when related to products used as elimination diets for the diagnosis of adverse food reaction (AFR) in dogs and cats because the presence of undeclared ingredients may negatively interfere with the trial and prevent the veterinarian from making an appropriate diagnosis...
June 27, 2018: BMC Veterinary Research
Thierry Olivry, Jennifer Bexley
BACKGROUND: Corn appears to be an uncommon food source of allergens in dogs and cats. There is limited information on the nature of the corn allergens in dogs and cats and their presence in the various foodstuffs used in commercial pet foods. The aim of this study was to determine if serum IgE from corn-sensitized dogs and cats recognized proteins in corn flour and cornstarch, which are common sources of carbohydrates in pet foods. RESULTS: We selected archived sera from allergy-suspected dogs (40) and cats (40) with either undetectable, low, medium or high serum levels of corn-specific IgE...
June 27, 2018: BMC Veterinary Research
Ronald H L Li, Lynelle R Johnson, Casey Kohen, Fern Tablin
BACKGROUND: Canine neutrophils release neutrophil extracellular traps (NETs) in response to lipopolysaccharide but NETs from clinical septic dogs had not been identified. The primary aim is to describe the methodology of identifying and quantifying neutrophil extracellular traps (NETs) in cytology samples of septic foci in dogs with sepsis using immunofluorescence microscopy. Cytology samples including endotracheal tracheal wash (ETW), bronchoalveolar lavage (BAL), abdominal and pleural effusion collected from 5 dogs (3 septic, 2 non-septic) were fixed, permeabilized and stained for myeloperoxidase (MPO), citrullinated histone H3 (citH3) and cell-free DNA (cfDNA)...
June 27, 2018: BMC Veterinary Research
M Mazlina, S Khairani-Bejo, H Hazilawati, T Tiagarahan, N N Shaqinah, M Zamri-Saad
BACKGROUND: This study was conducted to investigate the pathological changes and distribution of B. melitensis in the urinary tract of pregnant goats following acute experimental infection. Six Jamnapari crossbred does in their third trimester of pregnancy were randomly assigned into two groups; Group 1 was uninfected control and Group 2 was inoculated conjunctival with 0.1 mL of the inoculums containing 109  cfu/mL of live B. melitensis. All does were sacrificed 30 days post-inoculation before the kidney, ureter, urinary bladder, urethra and vaginal swab were collected for isolation of B...
June 25, 2018: BMC Veterinary Research
Lijiao Zhang, Zhanhong Li, Huan Jin, Xueying Hu, Jingliang Su
BACKGROUND: Tembusu virus (TMUV) is a member of the genus Flavivirus. Outbreak of this virus infection in duck flocks was first observed in China in April 2010, causing severe egg drop and neurological signs in laying ducks. Recently reported duck infections in southeastern Asia highlighted the need for well-validated diagnostic methods of TMUV surveillance to understand its epidemiological characteristics and maintenance in nature. Several enzyme-linked immunosorbent assays (ELISAs) for the detection of TMUV infection have been reported, but none have been applied to high-throughput diagnostics...
June 25, 2018: BMC Veterinary Research
T Martinello, C Gomiero, A Perazzi, I Iacopetti, F Gemignani, G M DeBenedictis, S Ferro, M Zuin, E Martines, P Brun, L Maccatrozzo, K Chiers, J H Spaas, M Patruno
BACKGROUND: Skin wound healing includes a system of biological processes, collectively restoring the integrity of the skin after injury. Healing by second intention refers to repair of large and deep wounds where the tissue edges cannot be approximated and substantial scarring is often observed. The objective of this study was to evaluate the effects of mesenchymal stem cells (MSCs) in second intention healing using a surgical wound model in sheep. MSCs are known to contribute to the inflammatory, proliferative, and remodeling phases of the skin regeneration process in rodent models, but data are lacking for large animal models...
June 25, 2018: BMC Veterinary Research
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