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BMC Veterinary Research

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https://www.readbyqxmd.com/read/30241533/combined-plasma-rich-in-growth-factors-and-adipose-derived-mesenchymal-stem-cells-promotes-the-cutaneous-wound-healing-in-rabbits
#1
Deborah Chicharro, Jose M Carrillo, Mónica Rubio, Ramón Cugat, Belén Cuervo, Silvia Guil, Jerónimo Forteza, Victoria Moreno, Jose M Vilar, Joaquín Sopena
BACKGROUND: The use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. In recent years, human and veterinary medicine prefer to avoid using traumatic techniques and choose low or non-invasive procedures. The objective of this study was to evaluate the efficacy of PRGF, ASCs and the combination of both in wound healing of full-thickness skin defects in rabbits. With this purpose, a total of 144 rabbits were used for this study...
September 21, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30241529/safety-and-immunogenicity-of-an-attenuated-chinese-pseudorabies-variant-by-dual-deletion-of-tk-ge-genes
#2
Jichun Wang, Zengcai Song, Aimin Ge, Rongli Guo, Yongfeng Qiao, Mengwei Xu, Zhisheng Wang, Yamei Liu, Yating Zheng, Hongjie Fan, Jibo Hou
BACKGROUND: Since the outbreak of a new emerging virulent pseudorabies virus mutant in Chinese pig herds, intensive research has been focused on the construction of novel gene deletion vaccine based on the variant virulent viruses. An ideal vaccine candidate is expected to have a balanced safety and immunogenicity. RESULTS: From the infectious clone of PRV AH02LA strain, a TK deletion mutant was generated through two-step Red mutagenesis. After homologous recombination with a transfer vector, a TK&gE dual deficient mutant PRV (PRVΔTK&gE-AH02 ) was generated, and its structure verified by PCR, RFLP and sequencing...
September 21, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30241522/tuberculosis-caused-by-mycobacterium-bovis-infection-in-a-captive-bred-american-bullfrog-lithobates-catesbeiana
#3
Cassia Yumi Ikuta, Laura Reisfeld, Bruna Silvatti, Fernanda Auciello Salvagni, Catia Dejuste de Paula, Allan Patrick Pessier, José Luiz Catão-Dias, José Soares Ferreira Neto
BACKGROUND: Tuberculosis is widely known as a progressive disease that affects endothermic animals, leading to death and/or economical losses, while mycobacterial infections in amphibians are commonly due to nontuberculous mycobacteria. To the authors' knowledge, this report describes the first case of bovine tuberculosis in a poikilothermic animal. CASE PRESENTATION: An adult female captive American bullfrog (Lithobates catesbeianus Shaw, 1802) died in a Brazilian aquarium...
September 21, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30231901/serological-and-entomological-survey-of-canine-leishmaniasis-in-lampedusa-island-italy
#4
Valentina Foglia Manzillo, Manuela Gizzarelli, Fabrizio Vitale, Serena Montagnaro, Alessandra Torina, Salvo Sotera, Gaetano Oliva
BACKGROUND: During last decade Lampedusa island (Italy) has been interested by a deep social change caused by the massive arrival of migrants from north Africa. The goal of this study was to evaluate current CanL burden and risk factors for Visceral Leishmaniosis (VL) on Lampedusa, actually based on very few data obtained in a previous study performed fifteen years ago. Two hundred and forty-two dogs were enrolled for the detection of Leishmania infantum infection by serology. In addition, an entomological investigation was performed to confirm the presence of Leishmania-vectors...
September 19, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30223839/assessing-changing-weather-and-the-el-ni%C3%A3-o-southern-oscillation-impacts-on-cattle-rabies-outbreaks-and-mortality-in-costa-rica-1985-2016
#5
Sabine E Hutter, Annemarie Käsbohrer, Silvia Lucia Fallas González, Bernal León, Katharina Brugger, Mario Baldi, L Mario Romero, Yan Gao, Luis Fernando Chaves
BACKGROUND: Rabies is a major zoonotic disease affecting humans, domestic and wildlife mammals. Cattle are the most important domestic animals impacted by rabies virus in the New World, leading to thousands of cattle deaths per year and eliciting large economic losses. In the New World, virus transmission in cattle is primarily associated with Desmodus rotundus, the common vampire bat. This study analyses the association of weather fluctuations and the El Niño Southern Oscillation (ENSO), with the occurrence and magnitude, in terms of associated mortality, of cattle rabies outbreaks...
September 17, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30223836/novel-protein-chip-for-the-detection-of-antibodies-against-infectious-bronchitis-virus
#6
Liping Yan, Jianhua Hu, Jing Lei, Zhiyu Shi, Qian Xiao, Zhenwei Bi, Lu Yao, Yuan Li, Yuqing Chen, An Fang, Hui Li, Suquan Song, Min Liao, Jiyong Zhou
BACKGROUND: Infectious bronchitis (IB) caused by the IB virus (IBV) can cause acute damage to chickens around the world. Therefore, rapid diagnosis and immune status determination are critical for controlling IBV outbreaks. Enzyme-linked immunosorbent assays (ELISAs) have been widely used in the detection of IBV antibodies in the early infection and continuous infection of IB because they are more sensitive and quicker than other diagnostic methods. RESULTS: We have developed two indirect microarray methods to detect antibodies against IBV: a chemiluminescent immunoassay test (CIT) and a rapid diagnostic test (RDT)...
September 17, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30208893/haemodynamic-changes-during-propofol-induction-in-dogs-new-findings-and-approach-of-monitoring
#7
Andrea Cattai, Roberto Rabozzi, Heidi Ferasin, Maurizio Isola, Paolo Franci
BACKGROUND: Propofol is one of the most widely used injectable anaesthetic agents in veterinary practice. Cardiovascular effects related to propofol use in dogs remain less well defined. The main objective of this study was to evaluate the haemodynamic changes during induction of general anaesthesia with propofol in healthy dogs, by a beat-to-beat continuous monitoring. All dogs were premedicated with intramuscular acepromazine (0.015 mg/kg) and methadone (0.15 mg/kg). Transthoracic echocardiography was used to measure the velocity time integral (VTI) of the left ventricular outflow tract...
September 12, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30208891/expression-of-selected-genes-isolated-from-whole-blood-liver-and-obex-in-lambs-with-experimental-classical-scrapie-and-healthy-controls-showing-a-systemic-innate-immune-response-at-the-clinical-end-stage
#8
Siv Meling, Kerstin Skovgaard, Kjetil Bårdsen, Peter Mikael Helweg Heegaard, Martha J Ulvund
BACKGROUND: Incubation period, disease progression, pathology and clinical presentation of classical scrapie in sheep are highly dependent on PRNP genotype, time and route of inoculation and prion strain. Our experimental model with pre-colostrum inoculation of homozygous VRQ lambs has shown to be an effective model with extensive PrPSc dissemination in lymphatic tissue and a short incubation period with severe clinical disease. Serum protein analysis has shown an elevation of acute phase proteins in the clinical stages of this experimental model, and here, we investigate changes in gene expression in whole blood, liver and brain...
September 12, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30208883/novel-insights-into-the-host-immune-response-of-chicken-harderian-gland-tissue-during-newcastle-disease-virus-infection-and-heat-treatment
#9
Perot Saelao, Ying Wang, Rodrigo A Gallardo, Susan J Lamont, Jack M Dekkers, Terra Kelly, Huaijun Zhou
BACKGROUND: Newcastle disease virus, in its most pathogenic form, threatens the livelihood of rural poultry farmers where there is a limited infrastructure and service for vaccinations to prevent outbreaks of the virus. Previously reported studies on the host response to Newcastle disease in chickens have not examined the disease under abiotic stressors, such as heat, which commonly experienced by chickens in regions such as Africa. The objective of this study was to elucidate the underlying biological mechanisms that contribute to disease resistance in chickens to the Newcastle disease virus while under the effects of heat stress...
September 12, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30208880/cross-contamination-in-canine-and-feline-dietetic-limited-antigen-wet-diets
#10
Elena Pagani, Maria de Los Dolores Soto Del Rio, Alessandra Dalmasso, Maria Teresa Bottero, Achille Schiavone, Liviana Prola
BACKGROUND: Adverse food reactions (AFRs) are defined as abnormal responses to an ingested food or food additive. Diagnosis and treatment of AFRs consist of the complete elimination of these ingredients in the dietary trial. Previous studies have demonstrated the presence of undeclared ingredients in commercial limited-antigen dry food diets that can compromise the results and efficacy of dietary elimination trails. The aim of this study was to assess a selection of commercial canine and feline dietetic limited-antigen wet foods for the potential cross-contamination of animal proteins from origins not mentioned on the label...
September 12, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30200962/characteristics-of-owned-dogs-in-rabies-endemic-kwazulu-natal-province-south-africa
#11
Melinda Hergert, Kevin Le Roux, Louis H Nel
BACKGROUND: Canine rabies has been enzootic in the dog population of the KwaZulu-Natal province of South Africa since the mid-1970s and has been associated with high rates of human exposures and frequent transmissions to other domestic animal species. Several decades of control efforts, consisting primarily of mass vaccination programs, have previously failed to sufficiently curb rabies in the province. Despite this history of canine rabies, the target canine population has never been extensively studied or quantified...
September 10, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30200958/neutralizing-antibodies-against-simbu-serogroup-viruses-in-cattle-and-sheep-nigeria-2012-2014
#12
Daniel Oluwayelu, Kerstin Wernike, Adebowale Adebiyi, Simeon Cadmus, Martin Beer
BACKGROUND: Simbu serogroup viruses of the Orthobunyavirus genus (Family Peribunyaviridae) include teratogenic pathogens that cause severe economic losses, abortions, stillbirths and congenital abnormalities in ruminants worldwide. Although they were initially isolated from ruminants and Culicoides biting midges about five decades ago in Nigeria, there is no current information on their prevalence and geographical distribution despite reports of abortions and congenital malformations in the country's ruminant population...
September 10, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30200953/sodium-butyrate-improves-antioxidant-stability-in-sub-acute-ruminal-acidosis-in-dairy-goats
#13
Nana Ma, Juma Ahamed Abaker, Muhammad Shahid Bilal, Hongyu Dai, Xiangzhen Shen
BACKGROUND: Currently, little is known about the effect of sodium butyrate (NaB) on oxidative stress following grain-induced sub-acute ruminal acidosis in dairy goats. In the present study, 18 lactating dairy goats implanted with a ruminal cannula and permanent indwelling catheters in the portal and hepatic veins were randomly allocated into 3 treatment groups over 20 weeks: low grain (LG, 40% grain; n = 6), high grain (HG, 60% grain; n = 6) and high grain with sodium butyrate (HG + NaB, 60% grain + NaB; n = 6)...
September 10, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30200947/coxiella-and-bartonella-spp-in-bats-chiroptera-captured-in-the-brazilian-atlantic-forest-biome
#14
Michelle Santos Ferreira, Alexandro Guterres, Tatiana Rozental, Roberto Leonan Morim Novaes, Emmanuel Messias Vilar, Renata Carvalho de Oliveira, Jorlan Fernandes, Danielle Forneas, Adonai Alvino Junior, Martha Lima Brandão, José Luis Passos Cordeiro, Martín Roberto Del Valle Alvarez, Sergio Luiz Althoff, Ricardo Moratelli, Pedro Cordeiro-Estrela, Rui Cerqueira da Silva, Elba Regina Sampaio de Lemos
BACKGROUND: The role of bats as reservoirs of zoonotic agents, especially pathogenic bacteria such as Bartonella and Coxiella, has been discussed around the world. Recent studies have identified bats as potential hosts of species from the proteobacteria phylum. In Brazil, however, the role of bats in the natural cycle of these agents is poorly investigated and generally neglected. In order to analyze the participation of bats in the epidemiology of diseases caused by Bartonella, Coxiella, Rickettsia, Anaplasma and Ehrlichia, we conducted a descriptive epidemiological study in three biogeographic regions of the Brazilian Atlantic Forest...
September 10, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30200937/esophageal-groove-dysfunction-a-cause-of-ruminal-bloat-in-newborn-calves
#15
Tamirat Kaba, Berhanu Abera, Temesgen Kassa
BACKGROUND: Esophageal groove dysfunction is one of the major causes of ruminal bloat. This condition is fatal in new born calves if it is not treated early. In healthy, suckling calves, milk should bypass the forestomach (rumen and reticulum) and enter into the abomasum where enzymatic digestion of milk proteins takes place. However, failure of the esophageal groove allows milk to enter into the forestomach, which results in the production of excess gases by microbial fermentation. Consequently, this increase in abdominal distention particularly on the left side in ruminants is an imminent manifestation of excess gases in the foresomach...
September 10, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30189884/molecular-and-serological-survey-of-lyssaviruses-in-croatian-bat-populations
#16
Ivana Šimić, Ivana Lojkić, Nina Krešić, Florence Cliquet, Evelyne Picard-Meyer, Marine Wasniewski, Anđela Ćukušić, Vida Zrnčić, Tomislav Bedeković
BACKGROUND: Rabies is the only known zoonotic disease of bat origin in Europe. The disease is caused by species belonging to the genus Lyssavirus. Five Lyssavirus species, i.e., European bat lyssavirus (EBLV)-1, EBLV-2, Bokeloh bat lyssavirus, Lleida bat lyssavirus, and West Caucasian bat virus, have been identified in European bats. More recently, a proposed sixth species, Kotalahti bat lyssavirus, was detected. Thus, in this study, active surveillance was initiated in order to obtain insights into the prevalence of lyssaviruses in Croatian bat populations and to improve our understanding of the public health threat of infected bats...
September 6, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30176863/a-new-bovine-tuberculosis-model-for-england-and-wales-botmew-to-simulate-epidemiology-surveillance-and-control
#17
Colin P D Birch, Ashley Goddard, Oliver Tearne
BACKGROUND: Bovine tuberculosis (bTB) is a zoonotic disease of cattle caused by Mycobacterium bovis, widespread in England and Wales. It has high incidence towards the South West of England and Wales, with much lower incidence to the East and North. A stochastic simulation model was developed to simulate M. bovis transmission among cattle, transfer by cattle movements and transmission from environmental reservoirs (often wildlife and especially badgers). It distinguishes five surveillance streams, including herd tests, pre-movement testing and slaughter surveillance...
September 4, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30176906/ex-vivo-preliminary-investigation-of-radiographic-quantitative-assessment-of-cranial-tibial-displacement-at-varying-degrees-of-canine-stifle-flexion-with-or-without-an-intact-cranial-cruciate-ligament
#18
Katrina A Castaneda, Caleb C Hudson, Brian S Beale
BACKGROUND: The presence of cranial tibial subluxation can aid in the detection of joint instability as a result of CrCL injury. Detection of cranial tibial subluxation has been described using the tibial compression test (TCT) and cranial drawer test (CDT); however, diagnosis of CrCL insufficiency by assessing cranial subluxation motion of the tibia is subjective and difficult to quantify accurately. The aim of this study was to investigate a measurement technique to assess the degree of cranial tibial displacement relative to the femoral condyles on mediolateral projection stifle radiographs at varying degrees of stifle flexion (90°, 110°, and 135°) in CrCL intact, partially, and completely transected conditions...
September 3, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30176905/photography-based-method-for-assessing-fluorescein-clearance-test-in-dogs
#19
Arianne Pontes Oriá, Miriam Flores Rebouças, Emanoel Martins Filho, Francisco de Assis Dórea Neto, Ana Cláudia Raposo, Lionel Sebbag
BACKGROUND: The fluorescein clearance test (FCT) provides insight into the tear film dynamics. The purpose of this study was to describe an inexpensive and practical method for assessing FCT in dogs, using photography and software analysis, and to assess the retention time of 1 vs. 2 eye drops on the canine ocular surface. METHODS: (i) In vivo - Eight healthy German Shepherd dogs were recruited. Following topical anesthesia with 0.5% proxymetacaine, each eye sequentially received (1 week apart) either 1 drop (35 μL) or 2 drops (70 μL) of 0...
September 3, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/30176903/specific-detection-of-muscovy-duck-parvovirus-infection-by-taqman-based-real-time-pcr-assay
#20
Chunhe Wan, Cuiteng Chen, Longfei Cheng, Hongmei Chen, Qiuling Fu, Shaohua Shi, Guanghua Fu, Rongchang Liu, Yu Huang
BACKGROUND: Muscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV...
September 3, 2018: BMC Veterinary Research
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