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Pakistan Journal of Biological Sciences: PJBS

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https://www.readbyqxmd.com/read/29023078/polymorphism-of-insulin-like-growth-factor-1-gene-igf1-tasi-igf1-snabi-igf1-rsai-and-the-association-with-daily-gain-of-pesisir-cattle-local-breed-from-west-sumatera-indonesia
#1
Yurnalis, Arnim, Dino Eka Putra
BACKGROUND AND OBJECTIVE: The insulin-like growth factor 1 gene (IGF1) is a candidate gene for marker-assisted selection strategies. Two single nucleotide polymorphism of IGF1 gene namely IGF1/SnaBI and IGF1/TasI has been reported to be associated with production traits in several cattle breeds. One single nucleotide polymorphism in exon 4 namely IGF1/Rsa1 has a significant influence (p<0.01) on birth weight, weaning weight and average daily gain of Bali cattle. The objective of this study was to determine the IGF1/SnaBI, IGF1/TasI and IGF1/RsaI polymorphisms and their associations with average daily gain in Pesisir cattle local breed from west Sumatera Indonesia...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023077/spermatogenic-and-phylo-molecular-characterizations-of-isolated-fasciola-spp-from-cattle-north-west-iran
#2
Soheila Rouhani, Saber Raeghi, Adel Spotin
BACKGROUND AND OBJECTIVE: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. MATERIALS AND METHODS: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023076/flavonoid-production-growth-and-differentiation-of-stelechocarpus-burahol-bl-hook-f-and-th-cell-suspension-culture
#3
Noor Aini Habibah, Sukarti Moeljopawiro, Kumala Dewi, Ari Indrianto
BACKGROUND AND OBJECTIVE: Stelechocarpus burahol is a plant containing flavonoid compounds that have the potential for use as an antihyperuricemic for gout medication. This study was performed to assess flavonoid production, growth and cell differentiation of S. burahol in cell suspension culture. METHODOLOGY: Mesocarp was planted in Murashige and Skoog (MS) medium supplemented with 7.5 mg L-1 picloram for the induction of callus. Non-embryonic callus obtained was used in the formation of cell suspension cultures...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023075/evaluation-of-mir-21-and-mir-10b-expression-of-human-breast-cancer-in-west-sumatera
#4
Yanwirasti, Wirsma A Harahap, Dessy Arisanty
BACKGROUND: Abnormal expression of several microRNAs (miRNAs) has been demonstrated in many types of cancer tumor tissue. The miR-10b and miR-21 are an oncogenic miRNAs which play role in proliferation and invasion of Breast Cancer (BC) tumorigenesis. The aim of this study was to evaluate the miR-21 and miR-10b expression in BC in West Sumatran women, Indonesia. MATERIALS AND METHODS: A total of 40 samples, consisting of 30 samples of breast cancer tissues (BC) and 10 samples of fibroadenoma tissues (FATs) as control and non-cancerous were analyzed...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023074/bacterial-quality-of-urinary-tract-infections-in-diabetic-and-non-diabetics-of-the-population-of-ma-an-province-jordan
#5
COMPARATIVE STUDY
Ali Al-Asoufi, Ali Khlaifat, Amjad Al Tarawneh, Khalid Alsharafa, Muhamad Al-Limoun, Khaled Khleifat
BACKGROUND AND OBJECTIVE: The patients with Diabetes Mellitus (DM) have malfunction in bladder which prompt urine accumulation in its pool which serves a decent situation to the microbes to be develop and cause Urinary Tract Infection (UTI). The UTI is the most infectious disease that affects both males and females. This study was designed to detect the bacterial species responsible for UTI in both diabetic and non-diabetic patients in Ma'an province, Jordan. MATERIALS AND METHODS: One hundred sixteen urine samples were investigated to determine UTI-causing bacteria...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023073/reproductive-biology-of-sardinella-sp-sardinella-aurita-and-sardinella-maderensis-in-the-south-of-morocco
#6
Ayoub Baali, Hajar Bourassi, Samira Falah, Wahbi Abderrazik, Khalid Manchih, Khadija Amenzoui, Ahmed Yahyaoui
BACKGROUND AND OBJECTIVE: Sardinella sp. has gained much attention lately because of its biomass increase, which might be the result of climatic changes occurring across the Atlantic sea. Little information is known about reproduction of these species particularly in the Moroccan Atlantic area. The objective of the this study was to explore some aspects of the reproductive biology of Sardinella in the South of Atlantic Moroccan coast. MATERIALS AND METHODS: Monthly samples were collected during the period between February, 2015 and January, 2016 in the area between Cape Boujdor and Cape Blanc...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023069/histological-characteristics-on-the-testes-of-mud-spiny-lobster-panulirus-polyphagus-herbst-1793
#7
Siti Nor Fatihah, Harman Muhd-Farouk, Adnan Amin-Safwan, Hairul Hafiz Mahsol, Mhd Ikhwanuddin
BACKGROUND AND OBJECTIVE: Mud spiny lobsters, Panulirus polyphagus (P. polyphagus) are one of the most important fisheries resources now-a-days due to quality that it's possessed. However, there is still lack of in-depth study about this species mainly on males testicular characteristics and sexual maturity size. Therefore, the present study was carried out to investigate the histological characteristics on the testes and sexual maturity sizes of mud spiny lobster, Panulirus polyphagus...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023068/zeatin-and-thidiazuron-induced-embryogenic-calli-from-in-vitro-leaf-and-stem-of-jojoba-simmondsia-chinensis
#8
Amal Abd El-Latif El-Ashry, Ahmed Mohamed Magdy Gabr, Shawky Abd El-Hamid Bekheet
BACKGROUND AND OBJECTIVE: Jojoba is a promising industrial plant, which recommended with pharmaceutical benefits. The present study was conducted to stimulate embryogenic calli formation from jojoba using zeatin and thidiazuron (TDZ), as well as determination of the antioxidant activity of proliferated calli. MATERIALS AND METHODS: For callus induction, leaf and stem explants derived from in vitro grown shootlets, were cultured on Murashige and Skoog (MS) medium with different combinations of 0...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023067/studies-on-staphylococcus-aureus-isolated-from-pimples
#9
Ajayi Adesola Adetutu, Bibi Oritsewehinmi, Oniha Margaret Ikhiwili, Atolagbe Oluwabunmi Moradeke, Anosike Selina Odochi, Onibokun Elizabeth Adeola
BACKGROUND AND OBJECTIVE: Pimples (acne) are small skin lesions or inflammations of the skin. The most common factor causing acne is the hormonal changes that occur during adolescent and teenage years. Antibiotics are becoming less effective in the treatment of pimples due to increasing concerns of antibiotic resistance. This study was therefore carried out to characterize the isolates from the pimples of Covenant University Students and to determine their antibiotics sensitivity pattern...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023066/%C3%AE-globin-alteration-in-%C3%AE-thalassemia-disorder-prediction-and-interaction-defect
#10
Niken Satuti Nur Handayani, Nailil Husna, Immanuel Sanka
BACKGROUND AND OBJECTIVE: The α-thalassemia is an inherited blood disorder affecting quality and quantity of hemoglobin. It caused mostly by deletion of one or two α-globin genes and characterized by deficient production of α-globin chain in hemoglobin leading from mild anemia to lethal. The α-globin gene with partial deletion could reduce chain production or produce abnormal chain. Its effect depends on mechanism of chain production affected. This study aimed to analyze the effect of partial deletion in α-globin gene influencing the mechanisms to produce functional α-globin chain in α-thalassemia cases...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023065/enzyme-digestive-activities-during-ontogeny-in-marosatherina-ladigesi-larvae
#11
Jayadi, Amrah Husma, Ilmia
BACKGROUND AND OBJECTIVE: Marosatherina ladigesi, including native fish in South Sulawesi Indonesia, are vulnerable and must be preserved. The objective of the study was to examine known food consumption, survival, growth and the activities of digestive enzymes of M. ladigesi larvae. MATERIAL AND METHODS: The larvae were obtained from natural spawning and the mixed live feed: Infusoria sp., Daphnia sp. and Artemia salina were given twice a day. Feed intake, growth, survival, activities of protease, lipase and amylase enzymes were observed in ages 3, 5, 10, 15, 20, 25, 30, 35 Days after Hatching (DAH)...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023064/impact-of-zeatin-and-thidiazuron-on-phenols-and-flavonoids-accumulation-in-callus-cultures-of-gardenia-gardenia-jasminoides
#12
Ahmed Mohamed Magdy Gabr, Nermeen Mohamed Arafa, Amal Abd El-Latif El-Ashry, Mohamed Kamal El-Bahr
BACKGROUND AND OBJECTIVE: Gardenia (Gardenia jasminoides) has many pharmacological actions such as anti-inflammatory, antioxidant and fibrolytic activities and cytotoxic effects, etc. This study was conducted to recognize the effect of zeatin and thidiazuron (TDZ) on callus proliferation, total phenolic content, total flavonoids and DPPH scavenging activity of gardenia callus cultures. MATERIALS AND METHODS: Calli were cultured on Murashige and Skoog (MS) medium supplement with different concentrations (2, 4 or 6 mg L-1) of zeatin or TDZ individually as well as combination of 2 mg L-1 zeatin+4 mg L-1 TDZ...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023063/effectiveness-of-metarhizium-anisopliae-and-entomopathogenic-nematodes-to-control-oryctes-rhinoceros-larvae-in-the-rainy-season
#13
Dyah Rini Indriyanti, Priyantini Widiyaningrum, Haryuni, Muji Slamet, Yoris Adi Maretta
BACKGROUND AND OBJECTIVE: Metarhizium anisopliae (MET) and entomopathogenic nematodes (EPN) are microorganisms that attack the larvae of Oryctes rhinoceros. The effects of MET, EPN and the combination of both on the O. rhinoceros larvae were studied during the rainy season in Jepara Indonesia. This study aimed to determine the effectiveness of Metarhizium anisopliae and entomopathogenic nematodes to control Oryctes rhinoceros larvae in the rainy season. MATERIALS AND METHODS: There were four level doses of MET, four level doses of EPN and four mixture of MET and EPN...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023062/biodegradation-of-quinalphos-by-a-soil-bacterium-bacillus-subtilis
#14
Venkata Subba Reddy Gangireddygari, DileepKumar Kanderi, Ramanjaneyulu Golla, Manjunatha Bangeppagari, Vijay Anand Kumar Babu Gundi, Khayalethu Ntushelo, Rajasekhar Reddy Bontha
BACKGROUND AND OBJECTIVE: A widely used pesticide quinalphos (O, O-diethyl O-quinoxalin-2-yl phosphorothioate) may be an undesirable and persistent pollutant to non-target environments like rivers and other ecosystems. The objective of this study was to isolate a potential degradant bacterium of quinalphos from polluted soils and test its fitness under various culture conditions. MATERIALS AND METHODS: A soil bacterium strain, capable of utilizing quinalphos as its sole source of carbon and energy was isolated from soil by enrichment method on a minimal salts medium (MSM)...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023061/protective-role-of-achillea-biebersteinii-pretreatment-on-dimethoate-induced-oxidative-stress-in-guinea-pigs-liver
#15
Yahya Saleh Al-Awthan, Mohamed Ali Al-Duais, Amany Abdulfatah Hazeb, Waleed Ahmed Alril
OBJECTIVE: The present study investigated the influence of Achillea biebersteinii (Ab), a medicinal herb used widely in Yemeni's folk medicine as analgesic, antipyretic, against diarrhea and flatulence and for liver diseases, on the liver antioxidant potential of guinea pigs acutely intoxicated with dimethoate pesticide (DM). MATERIALS AND METHODS: Animals were administered with Ab aqueous extract (50 and 100 mg kg-1 b.wt., orally) or silymarin (100 mg kg-1 b.wt...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023060/polymorphism-of-glutamate-cysteine-ligase-subunit-catalytic-gclc-gene-in-pulmonary-tuberculosis-patients
#16
Ari Yuniastuti, R Susanti, Dewi Mustikaningtyas
BACKGROUND AND OBJECTIVE: The biomarker of oxidative stress in pulmonary tuberculosis patients has not been found. Oxidative stress occurs due to the low level of antioxidants. Single nucleotide polymorphism of glutamate-cysteine ligase subunit catalytic (GCLC) gene namely -129C/T GCLC has been reported to have an association with a risk factor of oxidative stress' susceptibility. The Objective of this study was to determine the GCLC polymorphism in pulmonary tuberculosis (TB) patient...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023059/hematological-and-mineral-profiles-of-reproductive-failure-of-exotic-breed-cattle-in-payakumbuh-west-sumatra-indonesia
#17
Yuherman, Reswati, Yulianti Fitri Kurnia, Indahwati, Khalil
BACKGROUND AND OBJECTIVE: Female exotic breed cattle raised by traditional small farms are susceptible to reproductive disturbances that result in failure or delay to produce calves. The present research investigated the hematologic profiles of exotic breed cattle having reproductive troubles versus heifers and pregnant cows raised by small farms. MATERIALS AND METHODS: A survey was conducted to identify the reproduction performance of 160 female Simmental cows raised at 15 smallholders in Payakumbuh, West Sumatra, Indonesia...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023058/morphometric-characteristics-of-asian-catfish-hemibagrus-wyckii-bleeker-1858-bagridae-from-the-riau-province-of-indonesia
#18
Netti Aryani, Saberina Hasibuan, Ainul Mardiah, Hafrijal Syandri
BACKGROUND AND OBJECTIVE: Asian Catfish (Hemibagrus wyckii) is an important fish having both food and a high price in the market. These species are categorized as a carnivorous freshwater finfish native in Indonesia and is vulnerable to endangered status. This study was carried out to identify the morphometric characteristics of Hemibagrus wyckii (H. wyckii) from the Koto Panjang Reservoir, Kampar Kanan and Kampar Kiri rivers. MATERIALS AND METHODS: Twenty-five fish were collected from each sampling site...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023057/dynamic-of-bacterial-diversity-in-ileum-digesta-under-water-supplements-of-antibiotics-and-probiotics
#19
Raed MahmoudAl-Atiyat
BACKGROUND AND OBJECTIVE: Intestinal bacteria plays an important role in the physiological and immunological status of birds. The present study investigated the diversity of intestinal bacterial of broilers that were fed corn-soy diet and supplements of antibiotics and probiotics. MATERIALS AND METHODS: The supplemented treatments were water, water with antibiotics, water with probiotic and water with antibiotics and probiotic. Eight randomly sampled broilers were slaughtered and their ileum digesta taken...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023056/ameliorative-effect-of-arctium-lappa-against-cadmium-genotoxicity-and-histopathology-in-kidney-of-wistar-rat
#20
Asma Suliman Al-Gebaly
BACKGROUND AND OBJECTIVE: Cadmium (Cd) is a non-essential metal whose dispersion in the environment has increased recently, Cd may enhance cell oxidative stress that leads to DNA damage and apoptotic cell death. The study aimed to evaluate the antioxidative capability of Burdock root 'Arctium lappa' on cadmium-induced oxidative stress and histopathology of the kidney of Wistar rats. METHODOLOGY: Cadmium was applied in a form of cadmium chloride to three groups (15 mg Cd kg-1) for five weeks with two groups pre-treated with 'Arctium lappa' administration, 100 and 200 mg kg-1 b...
2017: Pakistan Journal of Biological Sciences: PJBS
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