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Pakistan Journal of Biological Sciences: PJBS

Mehrnoosh Maalhagh, Abdolreza Sotoodeh Jahromi, Alireza Yusefi, Ali Razeghi, Hassan Zabetiyan, Mohammad Yasin Karami, Abdol Hossein Madani
Stress has inhibitory effect on HPG axis through increasing cortisol serum level. In this study, the effect of acute prepubertal stress on kisspeptin, which plays essential role in puberty achievement is assessed. To do this experimental study thirty immature healthy male wistar rats of 4 weeks old and without any symptoms of puberty were selected randomly. These rats were divided into three groups, randomly. Two groups were chosen as control and pretest and one as stress (test) group. Immobilization stress was applied for 10 days and serum level of cortisol, testosterone and kisspeptin were measured...
January 2016: Pakistan Journal of Biological Sciences: PJBS
Bita Alain Mercier, Attibayéba, Kampé Jean Pierre, Ngantsoué Léon, Mialoundama Fidèle
Congolese forests contain important spontaneous food plants. Among these plants, there is the Grewia coriacea Mast., called in the national language "Tsui-téké", which is a tree of 4-25 m high and of 12-40 cm in diameter. Its fruits are used in several drinks making (juice, sparkling wine, syrup) and lollipops. Grewia's barks are used in pharmacopoeia to cure of stomach aches, syphilis. However, the fruits harvesting method based on branches or trees cutting as well as swidden agriculture by local people dangerously threatens the Grewia in the natural ecosystems of Congo...
January 2016: Pakistan Journal of Biological Sciences: PJBS
Badr E El-Bialy, Eman E Abdeen, Nermeen B El-Borai, Eman M El-Diasty
Aflatoxins (AFs), widely distributed food-borne mycotoxins, affect quality and safety of food and cause economic losses in livestock. In this study, the protective effect of Bee Pollen (BP) against some immunotoxic hazards elucidated from eating of AFs-containing diet was investigated in Wistar rats. Rats were randomly classified intofour groups and treated for 30 days, Group 1; control negative, Group 2; Total AFs (3 mg kg(-1) basal diet), Group 3; BP (20 g kg(-1) basal diet) and Group 4; AFs+BP in basal diet...
January 2016: Pakistan Journal of Biological Sciences: PJBS
Mutlag M Al-Otaibi, Jamal S Haddadin, Malik S Y Haddadin
Date fruit based products are gaining popularity among the consumers in almost all date growing countries due to its added nutritional value. Therefore, novel products were developed by combining two types of foods i.e., soft ripened cheeses and date fruit syrups or date powder. This study is the first to report the surface mold-ripened cheese production with date syrup and date powder. Model cheeses were prepared from pasteurized milk inoculated with Streptococcus thermophilus, Penicillium camemberti and Geotrichum candidum...
January 2016: Pakistan Journal of Biological Sciences: PJBS
Waleed S Alwaneen
Dairy industry is flourishing in Saudi Arabia for the last two decades producing milk and milk products to meet the population needs. Simultaneously, it is also producing large amount of dairy waste (animal manure) posing a serious environmental issues. Vermicomposting (conversion of animal manure into compost by bacterial treatments) is considered as one of the safest means for efficient management and to mitigate environmental pollution issues resulting from land disposal of raw dairy wastes. The main objective of this studywas to summarize different processes of vermicomposting and identified the most important bacteria species suitable for vermicomposting using animal manure especially the cowdung...
January 2016: Pakistan Journal of Biological Sciences: PJBS
R M Hifzan, Ismail Idris, H Yaakub
The objective of this research was to examine the growth pattern for body weight, body length and height at withers of Kalahari Red goats using non-linear models. The body size measurement data were collected from 227 Kalahari Red female goats and fit into Gompertz and Brody growth model. The results revealed that Gompertz growth model had the best goodness of fit to describe the growth of Kalahari Red goats for body weight, body length and height at withers as shown by higher coefficient of determination (97...
April 2015: Pakistan Journal of Biological Sciences: PJBS
Athar Rasekh, Hojjatollah Karimi Jashni, Karamatollah Rahmanian, Abdolreza Sotoodeh Jahromi
There is a rapidly growing trend in the consumption of herbal remedies in the developing countries. The aim of this study was to determine the effects of orally administered Date Palm Pollen (DPP) on the results of semen analysis in adult infertile men. Forty infertile men participated in our study. They were treated by Pollen powder 120 mg kg(-1) in gelatinous capsules every other day, for two months. Before and at the end of therapy, the semen was collected after masturbation and sperm numbers, motility and morphology were determined...
April 2015: Pakistan Journal of Biological Sciences: PJBS
I Achouri, Y Aboussaleh, R Sbaibi, A Ahami, M El Hioui
Iron deficiency anaemia is an important health problem in Morocco. This study was conducted to estimate the prevalence of anaemia among school children in Kenitra. The sample represents school children of all educational levels and age ranged between 6-15 years. The level of hemoglobin, haematocrit, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration was measured in a group of 271 school children. The seric iron was assessed and anaemia was defined when hemoglobin < 11...
April 2015: Pakistan Journal of Biological Sciences: PJBS
Chiranan Rattanabunta, Yupa Hanboonsong
The work consisted of two experiments, i.e. Experiment 1 was conducted under controlled environments where sugarcane plants were used as hosts. This investigation aimed to monitor the occurrence of the Sugarcane White Leaf disease and the abundance of Leafhopper insect vectors and also the work aimed to provide useful information in understanding some aspects on epidemiology of the Sugarcane White Leaf disease. A Completely Randomized Design with three replications was used to justify growth and development of Leafhopper insects as affected by different temperatures: 20 (T1), 25 (T2), 30 (T3) and 35 degrees C (T4)...
April 2015: Pakistan Journal of Biological Sciences: PJBS
H M Heikal
The present study was conducted at Elkom Elakhdar village, Shebin El-Kom, Menoufia Governorate along 2012 year seasons, to calculate and identify the species composition and the occurrence frequency of the extracted dust mites collected from three building ages at rural houses, as well as to determine the toxicity limits of different concentrations of three plant essential oils against two species of the family Pyroglyphidae the main causal of allergy to humans. The obtained results revealed that there were eleven mite species belong to five families (Pyroglyphidae, Chortoglyphidae, Glycyphagidae, Acaridae and Cheyletidae)...
April 2015: Pakistan Journal of Biological Sciences: PJBS
Suriya Jongyotha, Sompong Doolgindachbaporn, Prawit Suraniranat
Two experiments were carried out at the Department of Fisheries, Khon Kaen University, Khon Kaen, Thailand during September 2013 to February 2014 aiming to pursue both the better growth performance and survival rate of the freshwater prawns (Macrobrachium lanchesteri) where a Completely Randomized Design (CRD) with three replications was used for each experiment. For experiment 1, different percentages of grass shelter were used, i.e. 0% (Control, T1), 25% (T2), 50% (T3) and 75% (T4). For experiment 2, different percentages of commercial chitosan were used, i...
April 2015: Pakistan Journal of Biological Sciences: PJBS
O O Ajani, O B Familoni, D V Aderohunmu, K O Ogunniran, J A Adekoya, I O Olanrewaju
Reaction of p-toluenesulfonyl chloride with amino acids gave sulfonamides p-T1a-k which upon amidation afforded p-T2a-k. Similarly, treatment involving α-toluenesulfonyl chloride and amino acids afforded the sulfonamides α-T1a-k. These two classes of sulfonamides were synthetically modified at their COOH end position to achieve N,N-diethylamido substituted p-toluenesulfonamides p-T2a-k and α-toluenesulfonamides α-T2a-k, respectively. The chemical structures of the compounds were validated with IR, Mass spectra, NMR as well as elemental analytical data...
April 2015: Pakistan Journal of Biological Sciences: PJBS
Wuyi Liu, Fengmei Li
Xenopus laevis is a long established model organism for developmental, behavioral and neurological studies. Herein, an updated genome-wide survey was conducted using the ongoing genome project of Xenopus laevis and 106 non-redundant Basic Helix-Loop-Helix (bHLH) genes were identified in the Xenopus laevis genome databases. Gene Ontology (GO) enrichment statistics showed 51 significant GO annotations of biological processes and molecular functions and 5 significant KEGG pathways and a number of Xenopus laevis bHLH genes play significant role in specific development or special physiology processes like the development processes of muscle and eye and other organs...
April 2015: Pakistan Journal of Biological Sciences: PJBS
Y Irnidayanti
Heavy metals contamination on the coast of Jakarta Bay has led to the level of pollution and can cause toxicity to organisms living in the sea, i.e., green mussels. Green mussels have the ability to detoxify metals entering their bodies. Their ability to accumulate metals is higher than other aquatic animals. This is due to their sedentary life which prevents them from avoiding the effects of pollution and their high tolerance to certain metals. The high concentration of metal content would be toxic to the cell because metal ions can act as oxidants and bind to organic and protein molecules...
February 2015: Pakistan Journal of Biological Sciences: PJBS
Diah Ayu Satyari Utami, Widanarni, M Agus Suprayudi
The main things that need to be considered in the preparation of probiotics are viability during preparation and storage which are the disadvantages of the use of fresh culture probiotics. Dried probiotic can be applied through the feed, easy to be applied and has a long shelf life but application of dried probiotic in aquaculture is still not widely studied. This study aimed to evaluate the quality of dried Bacillus NP5 as the probiotic through in vitro assays and determine the best dose for the growth performance of tilapia...
February 2015: Pakistan Journal of Biological Sciences: PJBS
Jafar A Qasem, Salwa A Al-Mouqati, Ebtesam M Al-Ali, Ahmad Ben-Haji
Mycoplasma infection is a major problem in veterinary medicine and in poultry production. The pathogen has many strains, so that diagnosis of the disease using culture method is not effective. The objective of this work was to evaluate the prevalence of Mycoplasma gallisepticum (MG) in Kuwait poultry farms using serology and molecular methods in comparison to the culture under specific conditions. A total of 50 swab samples from choanal cleft and tracheal samples and blood samples were obtained from three different local farms, the blood samples were processed for an Enzyme Linked Immunosorbent Assay (ELISA) detection and the swab samples for Polymerase Chain Reaction (PCR) and culture methods detection...
February 2015: Pakistan Journal of Biological Sciences: PJBS
A A Ajayi, C F Peter-Albert, M Akeredolu, A A Shokunbi
Two varieties of tomato fruits commonly available in Nigerian markets are the Roma VF and Ibadan local varieties of tomato fruits. The Roma VF fruits are oval in shape. It is a common type of cultivar in the Northern region of Nigeria and it is not susceptible to cracking. The Ibadan local variety of tomato fruits is a local variety commonly found on farmers fields in South-western region of Nigeria. They are highly susceptible to cracking. The Ibadan local variety was employed for this research. There are lots of benefits derived from the consumption of tomato fruits...
February 2015: Pakistan Journal of Biological Sciences: PJBS
T D Adesina, O C Nwinyi, J A O Olugbuyiro
In this study, we investigated the feasibility of using Psidium guajava, Mangifera indica and Ocimum gratissimum leaf extracts in preventing Escherichia coli biofilm formation. The plants extractions were done with methanol under cold extraction. The various concentrations 5.0, 10.0 and 20.0 mg mL(-1) were used to coat 63 catheters under mild heat from water bath. Biofilm formation on the catheter was induced using cultures of E. coli. Biofilm formation was evaluated using aerobic plate count and turbidity at 600 nm...
February 2015: Pakistan Journal of Biological Sciences: PJBS
Widanarni, Tanbiyaskur
One of the fish diseases that is becoming the main problem in tilapia culture is streptococcosis caused by Streptococcus agalactiae. Application of probiotic, prebiotic and synbiotic are expected to be an alternative for controlling the disease. The purpose of this study was to examine the effectiveness of the administration of probiotic, prebiotic and synbiotic through artificial feed to control streptococcosis in tilapia. This study consisted of five treatments with three replications, namely positive control, negative control; 1% probiotic treatment; 2% prebiotic treatment and synbiotic treatment (1% probiotic and 2% prebiotic)...
February 2015: Pakistan Journal of Biological Sciences: PJBS
Abu Affan, Hisham S Khomavis, Salim Marzoog Al-Harbi, Mahfuzul Haque, Saleha Khan
Cyanobacterial blooms commonly appear during the summer months in ponds, lakes and reservoirs in Bangladesh. In these areas, fish mortality, odorous water and fish and human skin irritation and eye inflammation have been reported. The influence of physicochemical factors on the occurrence of cyanobacteria and its toxin levels were evaluated in natural and drinking water in Bangladesh. A highly sensitive immunosorbent assay was used to detect microcystins (MCs). Cyanobacteria were found in 22 of 23 samples and the dominant species were Microcystis aeruginosa, followed by Microcystisflosaquae, Anabeana crassa and Aphanizomenon flosaquae...
February 2015: Pakistan Journal of Biological Sciences: PJBS
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