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Medical Molecular Morphology

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https://www.readbyqxmd.com/read/29079935/periodontal-regeneration-induced-by-porous-alpha-tricalcium-phosphate-with-immobilized-basic-fibroblast-growth-factor-in-a-canine-model-of-2-wall-periodontal-defects
#1
Kazuya Matsuse, Yoshiya Hashimoto, Sachiro Kakinoki, Tetsuji Yamaoka, Shosuke Morita
We evaluated the effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on periodontal regeneration in a canine model of 2-wall periodontal defects. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), and the remaining defects were filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed at 2, 4, and 8 weeks post-implantation...
October 27, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28948378/a-comparative-study-between-platelet-rich-plasma-and-platelet-poor-plasma-effects-on-angiogenesis
#2
Minoo Shahidi, Mousa Vatanmakanian, Masoumeh Kourosh Arami, Fatemeh Sadeghi Shirazi, Najibeh Esmaeili, Samaneh Hydarporian, Soudeh Jafari
Platelet-rich plasma (PRP) has been established as an autologous source for therapeutic angiogenesis. The purpose of this study was to evaluate PRP angiogenic effects compared to platelet-poor plasma (PPP) in vitro and in vivo. The effects of PRP on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin...
September 25, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28887602/correlation-between-immunohistochemical-staining-of-ceacam1-and-clinicopathological-findings-in-oral-pre-neoplastic-lesions-and-squamous-cell-carcinoma
#3
Oriana Simonetti, Guendalina Lucarini, Corrado Rubini, Antonio Zizzi, Simone Domenico Aspriello, Roberto Di Primio, Anna Maria Offidani
Squamous cell carcinoma of the oral cavity represents the sixth most common cancer worldwide and it is often preceded by pre-neoplastic lesions. Sometimes it is still difficult for pathologists to make objective differential diagnoses only on histological characteristics. Tumorigenesis is accompanied by altered expression of cell adhesion molecules, like carcinoembryonic antigen cell adhesion molecule (CEACAM)1. We wanted to investigative CEACAM1 in oral dysplastic lesions, carcinoma in situ (CIS) and oral squamous cell carcinoma (OSCC)...
September 8, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28849340/insulinoma-associated-protein-1-insm1-is-a-useful-marker-for-pancreatic-neuroendocrine-tumor
#4
Masahiko Tanigawa, Masamichi Nakayama, Tomoki Taira, Satoshi Hattori, Yutaro Mihara, Reiichiro Kondo, Hironori Kusano, Ken Nakamura, Yushi Abe, Yusuke Ishida, Yoshinobu Okabe, Toru Hisaka, Koji Okuda, Kosuke Fujino, Takaaki Ito, Akihiko Kawahara, Yoshiki Naito, Rin Yamaguchi, Jun Akiba, Yoshito Akagi, Hirohisa Yano
Insulinoma-associated protein 1 (INSM1) is an important biomarker of Achaete-scute homolog-like 1-driven pathways. For diagnosis of pancreatic neuroendocrine tumors (PanNET), chromogranin A (CGA), synaptophysin (SYP), and neural cell adhesion molecule (NCAM) were also considered as potential biomarkers. However, it is often difficult to diagnose it immunohistochemically. Hence, we examined the expression pattern of INSM1 in pancreatic solid tumors. We detected INSM1, CGA, SYP, and NCAM immunohistochemically, in 27 cases of NET [pure type: 25 cases, mixed adenoneuroendocrine carcinoma (MANEC): 2 cases]...
August 28, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29067564/clinically-relevant-radioresistant-cell-line-a-simple-model-to-understand-cancer-radioresistance
#5
REVIEW
Yoshikazu Kuwahara, Mehryar Habibi Roudkenar, Yusuke Urushihara, Yohei Saito, Kazuo Tomita, Amaneh Mohammadi Roushandeh, Tomoaki Sato, Akihiro Kurimasa, Manabu Fukumoto
Radiotherapy (RT) is one of the major modalities for the treatment of human cancers and has been established as an excellent local treatment for malignant tumors. Conventional fractionated RT consists of 2-Gy X-rays, fractionated once a day, 5 days a week for 5-7 weeks in total 60 Gy. The efficacy of RT depends on the existence of radioresistant cells, which remains one of the most critical obstacles in RT and radio-chemotherapy. To improve the efficacy of RT, understanding the characteristics of radioresistant cells is one of the important subjects in radiation biology...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28936553/significance-of-evaluating-tumor-infiltrating-lymphocytes-tils-and-programmed-cell-death-ligand-1-pd-l1-expression-in-breast-cancer
#6
REVIEW
Sasagu Kurozumi, Takaaki Fujii, Hiroshi Matsumoto, Kenichi Inoue, Masafumi Kurosumi, Jun Horiguchi, Hiroyuki Kuwano
The immune system affects all phases of tumor growth from initiation to progression and dissemination. Tumor-infiltrating lymphocytes (TILs) are mononuclear immune cells that infiltrate tumor tissue. Several retrospective studies have suggested the potential of TILs as a prognostic as well as predictive factor of chemotherapy in some breast cancers. On the other hand, programmed cell death protein-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) eliminate T cell activation in various types of cancers. Prospective trials to evaluate the efficacy of antibody agents to PD-1 and PD-L1 are ongoing in patients with breast cancer...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28660384/increased-hepatic-abca1-transporter-is-associated-with-hypercholesterolemia-in-a-cholestatic-rat-model-and-primary-biliary-cholangitis-patients
#7
Yasuaki Takeyama, Yuko Uehara, Akira Anan, Daisuke Morihara, Keiji Yokoyama, Kazuhide Takata, Takashi Tanaka, Makoto Irie, Kaoru Iwata, Satoshi Shakado, Tetsuro Sohda, Shotaro Sakisaka
Hepatic ATP-binding cassette A1 (ABCA1) transporter is the modulator of intrahepatic cholesterol levels via the efflux of cholesterol into plasma. This study aimed to determine the expression of hepatic ABCA1 levels in a cholestatic rat model and patients with primary biliary cholangitis (PBC). A cholesterol efflux study was conducted with Abca1 knock down using siRNA in WIF9 cells. Cholesterol levels in the ABCA1 siRNA cells in the medium were significantly decreased compared with those in controls (P < 0...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28631038/the-significance-of-timd4-expression-in-clear-cell-renal-cell-carcinoma
#8
Hiromu Yano, Takanobu Motoshima, Chaoya Ma, Cheng Pan, Sohsuke Yamada, Toshiyuki Nakayama, Shohei Kitada, Naohiro Fujimoto, Tomomi Kamba, Motohiro Takeya, Yoshihiro Komohara
T-cell immunoglobulin and mucin domain (TIMD) family genes are related to innate immune responses. TIMD4 is a receptor for phosphatidylserine and is involved in the phagocytosis of apoptotic cells by macrophages. In the present study, we found that TIMD4 is expressed on the cancer cells of patients with clear cell renal cell carcinoma (ccRCC). TIMD4 was immunostained in the resected samples of 89 patients diagnosed as ccRCC. High expression of TIMD4 in cancer cells was closely related to short progression free survival time; however, it was not correlated with other clinicopathological factors...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28631037/inclusion-bodies-of-aggregated-hemosiderins-in-liver-macrophages
#9
Hisao Hayashi, Yasuaki Tatsumi, Shinya Wakusawa, Ryota Shigemasa, Ryoji Koide, Ken-Ichi Tsuchida, Natsuko Morotomi, Tetsuji Yamashita, Kotaro Kumagai, Yukiya Ono, Kazuhiko Hayashi, Masatoshi Ishigami, Hidemi Goto, Ayako Kato, Koichi Kato
Hemosiderin formation is a structural indication of iron overload. We investigated further adaptations of the liver to excess iron. Five patients with livers showing iron-rich inclusions larger than 2 µm were selected from our database. The clinical features of patients and structures of the inclusions were compared with those of 2 controls with mild iron overload. All patients had severe iron overload with more than 5000 ng/mL of serum ferritin. Etiologies were variable, from hemochromatosis to iatrogenic iron overload...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28577062/hepatic-progenitor-cell-expansion-in-early-stage-nonalcoholic-steatohepatitis-evidence-from-immunohistochemistry-and-immunoelectron-microscopy-of-matrix-metalloproteinase-1
#10
LETTER
Hiroaki Yokomori, Masaya Oda, Wataru Ando, Yutaka Inagaki, Isao Okazaki
No abstract text is available yet for this article.
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28516286/establishment-of-a-tumor-sphere-cell-line-from-a-metastatic-brain-neuroendocrine-tumor
#11
Ryoichi Iwata, Masato Maruyama, Tomoki Ito, Yosuke Nakano, Yonehiro Kanemura, Taro Koike, Souichi Oe, Kunikazu Yoshimura, Masahiro Nonaka, Shosaku Nomura, Tetsuo Sugimoto, Hisao Yamada, Akio Asai
Neuroendocrine tumors are rare, and little is known about the existence of cancer stem cells in this disease. Identification of the tumorigenic population will contribute to the development of effective therapies targeting neuroendocrine tumors. Surgically resected brain metastases from a primary neuroendocrine tumor of unknown origin were dissociated and cultured in serum-free neurosphere medium. Stem cell properties, including self-renewal, differentiation potential, and stem cell marker expression, were examined...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28534217/autism-spectrum-disorder-associated-genes-and-the-development-of-dentate-granule-cells
#12
REVIEW
Hidenori Ito, Rika Morishita, Koh-Ichi Nagata
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by severe clinical symptoms such as the deficiency of the social communication, repetitive and stereotyped behaviors, and restricted interests. Although complex genetic and environmental factors are thought to contribute to the development of ASD, the precise etiologies are largely unknown. Neuroanatomical observations have been made of developmental abnormalities in different brain regions, including dentate gyrus of hippocampus, which is widely accepted as the center for learning and memory...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28516285/intracellular-morphological-changes-in-staphylococcus-aureus-induced-by-treatment-with-sodium-hypochlorite
#13
Shiori Ujimine, Shigenobu Tone, Mineki Saito, Sakuo Yamada
Sodium hypochlorite (NaOCl) is commonly used as a disinfectant; however, its bactericidal mechanism has not yet been clarified. In the present study, the bactericidal mechanism of NaOCl was examined using microscopy and gel electrophoresis techniques with Staphylococcus aureus strain 209P. S. aureus cells treated with 500 and 1000 ppm NaOCl for 5 and 15 min were observed by SEM and TEM. SEM images of the bacterial cells treated with NaOCl showed an irregular surface, with cells being partially invaginated...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28439674/adipose-derived-stem-cell-asc-enriched-fat-grafting-experiments-using-white-rabbits-and-an-automated-cell-processing-apparatus
#14
Natsuko Kakudo, Naoki Morimoto, Takeshi Ogawa, Masakatsu Hihara, Fangyuan Lai, Kenji Kusumoto
The grafting of fat mixed with adipose-derived stem cells (ASCs) is being increasingly applied to compensate for the disadvantages of previous fat grafting methods. Devices that automatically isolate fat stem cells also have recently been developed. ASCs were isolated from the inguinal region of White rabbits using Icellator(®), and the number of cells and their viability were measured. The cell count per fat graft (mL) was adjusted to the following concentrations and subcutaneously transplanted into the back: Control group, Fat + PBS; Fat + ASCs (×0...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28382533/quantified-kidney-echogenicity-in-mice-with-renal-ischemia-reperfusion-injury-evaluation-as-a-noninvasive-biomarker-of-acute-kidney-injury
#15
Shinya Murata, Noriyuki Sugiyama, Kentaro Maemura, Yoshinori Otsuki
The purpose is to evaluate quantified kidney echogenicity as a biomarker for the early diagnosis of acute kidney injury (AKI) and predicting progression to chronic kidney disease (CKD) in a mouse model of ischemia-reperfusion injury (IRI). Two separate protocols of murine models of IRI were used: (1) 10, 30, and 40 min of bilateral ischemia duration and (2) 45 and 60 min of unilateral ischemia duration. Renal echogenicity was measured with ultrasound and compared with serum creatinine or urine neutrophil gelatinase-associated lipocalin (NGAL) at various timepoints after IRI...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28353090/tissue-inhibitors-of-metalloproteinase-expressing-cells-in-human-anterior-pituitary-and-pituitary-adenoma
#16
Alimuddin Tofrizal, Ken Fujiwara, Morio Azuma, Motoshi Kikuchi, Depicha Jindatip, Takashi Yashiro, Shozo Yamada
Extracellular matrix (ECM) is essential in tissue physiology and pathologic conditions such as tumorigenesis. It affects tumor cell behavior, proliferation, and metastasis. Pituitary adenomas differ in their clinical characteristics, including ECM deposition, and we recently reported that the characteristics of collagen-producing cells differed between control human anterior pituitary gland and pituitary adenomas. ECM deposition is not defined solely by production; degradation and maintenance are also important...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28303350/immunohistochemical-evaluation-of-klotho-and-dna-methyltransferase-3a-in-oral-squamous-cell-carcinomas
#17
Bhoj Raj Adhikari, Osamu Uehara, Hirofumi Matsuoka, Rie Takai, Fumiya Harada, Masafumi Utsunomiya, Takatoshi Chujo, Tetsuro Morikawa, Mamata Shakya, Koki Yoshida, Jun Sato, Toshiya Arakawa, Michiko Nishimura, Hiroki Nagayasu, Itsuo Chiba, Yoshihiro Abiko
Carcinoma follows a course of multiple changes that are affected by several important factors, with epigenetic silencing of the promoter gene being one of them. A series of studies have suggested that epigenetic changes in the anti-aging gene Klotho may be one of the emerging areas of concern in the study of carcinogenesis. We hypothesized that epigenetic silencing of Klotho due to hypermethylation of DNMT3a may be one of the causes of carcinoma in the oral and maxillofacial region. In this study, we analyzed the immunohistochemical expressions of Klotho and DNMT3a in tissues obtained from oral dysplasia and oral squamous cell carcinoma...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28247010/pathological-and-molecular-analyses-of-atherosclerotic-lesions-in-apoe-knockout-mice
#18
Masa-Aki Shibata, Eiko Shibata, Kentaro Maemura, Yoichi Kondo, Mariko Harada-Shiba
The establishment of consistent and reliable methods for the analysis of atherosclerosis molecular pathways and for testing the efficiency of new therapeutics is of utmost importance. Here, we fed ApoE-knockout (KO) mice with high-fat diet to for 16 weeks to induce atherosclerosis. Atherosclerotic lesions in mice were methodically investigated using pathologic analyses and molecular biology tools. These lesions were histopathologically classified into three categories: early, progressive, and combined lesions...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28687956/cd163-positive-cancer-cells-are-potentially-associated-with-high-malignant-potential-in-clear-cell-renal-cell-carcinoma
#19
Chaoya Ma, Hasita Horlad, Koji Ohnishi, Takenobu Nakagawa, Sohsuke Yamada, Shohei Kitada, Takanobu Motoshima, Tomomi Kamba, Toshiyuki Nakayama, Naohiro Fujimoto, Motohiro Takeya, Yoshihiro Komohara
CD163 is preferentially expressed by monocyte/macrophages; however, recent studies using immunohistochemistry (IHC) have reported that some cancer cells also express CD163. In the present IHC study, we investigated CD163 staining of cancer cells and macrophages in clear cell renal cell carcinoma (ccRCC) tissues and determined the relationship between cancer cell CD163 expression and clinical prognosis in patients with ccRCC. IHC for CD163 was performed in ccRCC tissues from 103 patients. CD163-positive cancer cells were detected in 35% of the patients (36/103); however, the positive signals on cancer cells were significantly lower than those on macrophages...
July 7, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28314927/enhanced-podocyte-vesicle-transport-in-the-nephrotic-rat
#20
Akihiro Tojo, Saaya Hatakeyama, Satoshi Kinugasa, Satoru Fukuda, Toshio Sakai
Albumin endocytosis is enhanced in the podocytes of minimal change nephrotic syndrome. We investigated that the endocytic vesicle transport in the podocyte using three-dimensional observation in a rat model of puromycin aminonucleoside (PAN)-induced nephrotic syndrome. At day 7, Evans Blue-labeled albumin was intravenously injected in PAN rats, and one kidney was fixed for a morphological analysis; the other was used for the isolation of glomeruli through sieving and protein analyses. Evans Blue-labeled albumin was found to accumulate in an increased number of vesicles in the podocytes of PAN rat...
June 2017: Medical Molecular Morphology
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