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Medical Molecular Morphology

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https://www.readbyqxmd.com/read/29318388/h-atpase-blockade-reduced-renal-gluconeogenesis-and-plasma-glucose-in-a-diabetic-rat-model
#1
Akihiro Tojo, Saaya Hatakeyama, Masaomi Nangaku, Toshihiko Ishimitsu
Vacuolar H+-adenosine triphosphatase (ATPase) plays important roles in urinary acid excretion, vesicular acidification to activate enzymes, and the membrane recycling of transporters in the kidney. As acidosis stimulates renal gluconeogenesis, we investigated the effect of blockade of H+-ATPase on renal gluconeogenesis in diabetic rats. Diabetes mellitus was induced by a single injection of streptozotocin, and a group of DM rats was treated with bafilomycin B1 intraperitoneally for 8 days. In diabetic rats, the renal expression and activity of H+-ATPase were increased with elevated urinary ammonium excretion...
January 9, 2018: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29282541/creatine-phosphate-disodium-salt-protects-against-dox-induced-cardiotoxicity-by-increasing-calumenin
#2
Yu Wang, Ying Sun, Xin Guo, Yao Fu, Jie Long, Cheng-Xi Wei, Ming Zhao
Inhibiting endoplasmic reticulum stress (ERS)-induced apoptosis may be a new therapeutic target in cardiovascular diseases. Creatine phosphate disodium salt (CP) has been reported to have cardiovascular protective effect, but its effects on ERS are unknown. The aim of this study was to identify the mechanism by which CP exerts its cardioprotection in doxorubicin (Dox)-induced cardiomyocytes injury. In our study, neonatal rats cardiomyocytes (NRC) was randomly divided into control group, model group, and treatment group...
December 27, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29282540/expression-analyses-of-dusp22-dual-specificity-phosphatase-22-in-mouse-tissues
#3
Nanako Hamada, Makoto Mizuno, Hiroyuki Tomita, Ikuko Iwamoto, Akira Hara, Koh-Ichi Nagata
Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton...
December 27, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29280012/suox-is-negatively-associated-with-multistep-carcinogenesis-and-proliferation-in-oral-squamous-cell-carcinoma
#4
Ken Nakamura, Jun Akiba, Sachiko Ogasawara, Yoshiki Naito, Masamichi Nakayama, Yushi Abe, Jingo Kusukawa, Hirohisa Yano
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor in the head and neck region. The aim of this study was to identify the key molecules and to elucidate the molecular mechanisms of OSCC carcinogenesis through a microarray analysis of RNA extracted from normal epithelium, dysplasia, and squamous cell carcinoma components. Out of molecules that showed changes in gene expression in the microarray analysis, we focused on Sulfite oxidase (SUOX), which correlated significantly with carcinogenic process and exhibited a stepwise decrease in expression...
December 26, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29218457/phenotypical-change-of-tumor-associated-macrophages-in-metastatic-lesions-of-clear-cell-renal-cell-carcinoma
#5
Takanobu Motoshima, Yuji Miura, Nanako Wakigami, Natsuki Kusada, Toshimi Takano, Naoko Inoshita, Toshikazu Okaneya, Yutaka Sugiyama, Tomomi Kamba, Motohiro Takeya, Yoshihiro Komohara
Macrophages are the main immune cells of the tumor microenvironment in clear cell renal cell carcinoma (ccRCC). A high density of CD163+ or CD204+ tumor-associated macrophages (TAMs), rather than the density of total TAMs, is known to be linked to poor clinical outcome. In the present study, we investigated the phenotypical differences between the paired primary and metastatic lesions in ccRCC cases. Using immunostaining, the densities of CD163+ and CD204+ TAMs in metastatic lesions were found to be significantly lower compared to primary lesions, although the total number of TAMs was increased in metastatic lesions...
December 7, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29170827/the-role-of-nanomaterials-in-cell-delivery-systems
#6
REVIEW
Ali Golchin, Simzar Hosseinzadeh, Leila Roshangar
In more than one decade, cell transplantation has created an important strategy to treat a wide variety of diseases characterized by tissue and cell dysfunctions. In this course of action, cell delivery to target site has been always one of the most important constraints and complications, as only a small proportion of the cells are housed in the target sites. Nanotechnology and nanoscale biomaterials have been helpful for cell transplantation in various fields of regenerative medicine including diagnosis, delivery systems for the cell, drug or gene, and cells protection system...
November 23, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29079935/periodontal-regeneration-induced-by-porous-alpha-tricalcium-phosphate-with-immobilized-basic-fibroblast-growth-factor-in-a-canine-model-of-2-wall-periodontal-defects
#7
Kazuya Matsuse, Yoshiya Hashimoto, Sachiro Kakinoki, Tetsuji Yamaoka, Shosuke Morita
We evaluated the effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on periodontal regeneration in a canine model of 2-wall periodontal defects. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), and the remaining defects were filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed at 2, 4, and 8 weeks post-implantation...
October 27, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29067564/clinically-relevant-radioresistant-cell-line-a-simple-model-to-understand-cancer-radioresistance
#8
REVIEW
Yoshikazu Kuwahara, Mehryar Habibi Roudkenar, Yusuke Urushihara, Yohei Saito, Kazuo Tomita, Amaneh Mohammadi Roushandeh, Tomoaki Sato, Akihiro Kurimasa, Manabu Fukumoto
Radiotherapy (RT) is one of the major modalities for the treatment of human cancers and has been established as an excellent local treatment for malignant tumors. Conventional fractionated RT consists of 2-Gy X-rays, fractionated once a day, 5 days a week for 5-7 weeks in total 60 Gy. The efficacy of RT depends on the existence of radioresistant cells, which remains one of the most critical obstacles in RT and radio-chemotherapy. To improve the efficacy of RT, understanding the characteristics of radioresistant cells is one of the important subjects in radiation biology...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28936553/significance-of-evaluating-tumor-infiltrating-lymphocytes-tils-and-programmed-cell-death-ligand-1-pd-l1-expression-in-breast-cancer
#9
REVIEW
Sasagu Kurozumi, Takaaki Fujii, Hiroshi Matsumoto, Kenichi Inoue, Masafumi Kurosumi, Jun Horiguchi, Hiroyuki Kuwano
The immune system affects all phases of tumor growth from initiation to progression and dissemination. Tumor-infiltrating lymphocytes (TILs) are mononuclear immune cells that infiltrate tumor tissue. Several retrospective studies have suggested the potential of TILs as a prognostic as well as predictive factor of chemotherapy in some breast cancers. On the other hand, programmed cell death protein-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) eliminate T cell activation in various types of cancers. Prospective trials to evaluate the efficacy of antibody agents to PD-1 and PD-L1 are ongoing in patients with breast cancer...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28660384/increased-hepatic-abca1-transporter-is-associated-with-hypercholesterolemia-in-a-cholestatic-rat-model-and-primary-biliary-cholangitis-patients
#10
Yasuaki Takeyama, Yuko Uehara, Akira Anan, Daisuke Morihara, Keiji Yokoyama, Kazuhide Takata, Takashi Tanaka, Makoto Irie, Kaoru Iwata, Satoshi Shakado, Tetsuro Sohda, Shotaro Sakisaka
Hepatic ATP-binding cassette A1 (ABCA1) transporter is the modulator of intrahepatic cholesterol levels via the efflux of cholesterol into plasma. This study aimed to determine the expression of hepatic ABCA1 levels in a cholestatic rat model and patients with primary biliary cholangitis (PBC). A cholesterol efflux study was conducted with Abca1 knock down using siRNA in WIF9 cells. Cholesterol levels in the ABCA1 siRNA cells in the medium were significantly decreased compared with those in controls (P < 0...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28631038/the-significance-of-timd4-expression-in-clear-cell-renal-cell-carcinoma
#11
Hiromu Yano, Takanobu Motoshima, Chaoya Ma, Cheng Pan, Sohsuke Yamada, Toshiyuki Nakayama, Shohei Kitada, Naohiro Fujimoto, Tomomi Kamba, Motohiro Takeya, Yoshihiro Komohara
T-cell immunoglobulin and mucin domain (TIMD) family genes are related to innate immune responses. TIMD4 is a receptor for phosphatidylserine and is involved in the phagocytosis of apoptotic cells by macrophages. In the present study, we found that TIMD4 is expressed on the cancer cells of patients with clear cell renal cell carcinoma (ccRCC). TIMD4 was immunostained in the resected samples of 89 patients diagnosed as ccRCC. High expression of TIMD4 in cancer cells was closely related to short progression free survival time; however, it was not correlated with other clinicopathological factors...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28631037/inclusion-bodies-of-aggregated-hemosiderins-in-liver-macrophages
#12
Hisao Hayashi, Yasuaki Tatsumi, Shinya Wakusawa, Ryota Shigemasa, Ryoji Koide, Ken-Ichi Tsuchida, Natsuko Morotomi, Tetsuji Yamashita, Kotaro Kumagai, Yukiya Ono, Kazuhiko Hayashi, Masatoshi Ishigami, Hidemi Goto, Ayako Kato, Koichi Kato
Hemosiderin formation is a structural indication of iron overload. We investigated further adaptations of the liver to excess iron. Five patients with livers showing iron-rich inclusions larger than 2 µm were selected from our database. The clinical features of patients and structures of the inclusions were compared with those of 2 controls with mild iron overload. All patients had severe iron overload with more than 5000 ng/mL of serum ferritin. Etiologies were variable, from hemochromatosis to iatrogenic iron overload...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28577062/hepatic-progenitor-cell-expansion-in-early-stage-nonalcoholic-steatohepatitis-evidence-from-immunohistochemistry-and-immunoelectron-microscopy-of-matrix-metalloproteinase-1
#13
LETTER
Hiroaki Yokomori, Masaya Oda, Wataru Ando, Yutaka Inagaki, Isao Okazaki
No abstract text is available yet for this article.
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28516286/establishment-of-a-tumor-sphere-cell-line-from-a-metastatic-brain-neuroendocrine-tumor
#14
Ryoichi Iwata, Masato Maruyama, Tomoki Ito, Yosuke Nakano, Yonehiro Kanemura, Taro Koike, Souichi Oe, Kunikazu Yoshimura, Masahiro Nonaka, Shosaku Nomura, Tetsuo Sugimoto, Hisao Yamada, Akio Asai
Neuroendocrine tumors are rare, and little is known about the existence of cancer stem cells in this disease. Identification of the tumorigenic population will contribute to the development of effective therapies targeting neuroendocrine tumors. Surgically resected brain metastases from a primary neuroendocrine tumor of unknown origin were dissociated and cultured in serum-free neurosphere medium. Stem cell properties, including self-renewal, differentiation potential, and stem cell marker expression, were examined...
December 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28948378/a-comparative-study-between-platelet-rich-plasma-and-platelet-poor-plasma-effects-on-angiogenesis
#15
Minoo Shahidi, Mousa Vatanmakanian, Masoumeh Kourosh Arami, Fatemeh Sadeghi Shirazi, Najibeh Esmaeili, Samaneh Hydarporian, Soudeh Jafari
Platelet-rich plasma (PRP) has been established as an autologous source for therapeutic angiogenesis. The purpose of this study was to evaluate PRP angiogenic effects compared to platelet-poor plasma (PPP) in vitro and in vivo. The effects of PRP on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin...
September 25, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28887602/correlation-between-immunohistochemical-staining-of-ceacam1-and-clinicopathological-findings-in-oral-pre-neoplastic-lesions-and-squamous-cell-carcinoma
#16
Oriana Simonetti, Guendalina Lucarini, Corrado Rubini, Antonio Zizzi, Simone Domenico Aspriello, Roberto Di Primio, Anna Maria Offidani
Squamous cell carcinoma of the oral cavity represents the sixth most common cancer worldwide and it is often preceded by pre-neoplastic lesions. Sometimes it is still difficult for pathologists to make objective differential diagnoses only on histological characteristics. Tumorigenesis is accompanied by altered expression of cell adhesion molecules, like carcinoembryonic antigen cell adhesion molecule (CEACAM)1. We wanted to investigative CEACAM1 in oral dysplastic lesions, carcinoma in situ (CIS) and oral squamous cell carcinoma (OSCC)...
September 8, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28534217/autism-spectrum-disorder-associated-genes-and-the-development-of-dentate-granule-cells
#17
REVIEW
Hidenori Ito, Rika Morishita, Koh-Ichi Nagata
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by severe clinical symptoms such as the deficiency of the social communication, repetitive and stereotyped behaviors, and restricted interests. Although complex genetic and environmental factors are thought to contribute to the development of ASD, the precise etiologies are largely unknown. Neuroanatomical observations have been made of developmental abnormalities in different brain regions, including dentate gyrus of hippocampus, which is widely accepted as the center for learning and memory...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28516285/intracellular-morphological-changes-in-staphylococcus-aureus-induced-by-treatment-with-sodium-hypochlorite
#18
Shiori Ujimine, Shigenobu Tone, Mineki Saito, Sakuo Yamada
Sodium hypochlorite (NaOCl) is commonly used as a disinfectant; however, its bactericidal mechanism has not yet been clarified. In the present study, the bactericidal mechanism of NaOCl was examined using microscopy and gel electrophoresis techniques with Staphylococcus aureus strain 209P. S. aureus cells treated with 500 and 1000 ppm NaOCl for 5 and 15 min were observed by SEM and TEM. SEM images of the bacterial cells treated with NaOCl showed an irregular surface, with cells being partially invaginated...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28439674/adipose-derived-stem-cell-asc-enriched-fat-grafting-experiments-using-white-rabbits-and-an-automated-cell-processing-apparatus
#19
Natsuko Kakudo, Naoki Morimoto, Takeshi Ogawa, Masakatsu Hihara, Fangyuan Lai, Kenji Kusumoto
The grafting of fat mixed with adipose-derived stem cells (ASCs) is being increasingly applied to compensate for the disadvantages of previous fat grafting methods. Devices that automatically isolate fat stem cells also have recently been developed. ASCs were isolated from the inguinal region of White rabbits using Icellator(®), and the number of cells and their viability were measured. The cell count per fat graft (mL) was adjusted to the following concentrations and subcutaneously transplanted into the back: Control group, Fat + PBS; Fat + ASCs (×0...
September 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28382533/quantified-kidney-echogenicity-in-mice-with-renal-ischemia-reperfusion-injury-evaluation-as-a-noninvasive-biomarker-of-acute-kidney-injury
#20
Shinya Murata, Noriyuki Sugiyama, Kentaro Maemura, Yoshinori Otsuki
The purpose is to evaluate quantified kidney echogenicity as a biomarker for the early diagnosis of acute kidney injury (AKI) and predicting progression to chronic kidney disease (CKD) in a mouse model of ischemia-reperfusion injury (IRI). Two separate protocols of murine models of IRI were used: (1) 10, 30, and 40 min of bilateral ischemia duration and (2) 45 and 60 min of unilateral ischemia duration. Renal echogenicity was measured with ultrasound and compared with serum creatinine or urine neutrophil gelatinase-associated lipocalin (NGAL) at various timepoints after IRI...
September 2017: Medical Molecular Morphology
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