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Journal of Zhejiang University. Science. B

Roxana Karbaschi, Forouzan Sadeghimahalli, Homeira Zardooz
This study attempts to further clarify the potential effects of maternal high-fat (HF) diet on glucose homeostasis in dams and young adult male rat offspring. Female rats were divided into control (CON dams) and HF (HF dams) diet groups, which received the diet 4 weeks prior to and through pregnancy and lactation periods. Blood samples were taken to determine metabolic parameters, then an intraperitoneal glucose tolerance test (IPGTT) was performed. Maternal HF diet increased intra-abdominal fat mass and plasma corticosterone level, but decreased leptin concentration in dams...
September 2016: Journal of Zhejiang University. Science. B
Shi-Cheng Yu, Hai-Yang Wu, Wei Wang, Li-Wei Xu, Guo-Qing Ding, Zhi-Gen Zhang, Gong-Hui Li
OBJECTIVES: We retrospectively reviewed the urethral stricture cases treated in our tertiary center, and assessed the safety and feasibility of the high-pressure balloon dilation (HPBD) technique for anterior urethral stricture. METHODS: From January 2009 to December 2012, a total of 31 patients with anterior urethral strictures underwent HPBD at our center, while another 25 cases were treated by direct vision internal urethrotomy (DVIU). Patient demographics, stricture characteristics, surgical techniques, and operative outcomes were assessed and compared between the two groups...
September 2016: Journal of Zhejiang University. Science. B
Liu-Xin Cai, Fang-Qiang Wei, Yi-Chen Yu, Xiu-Jun Cai
OBJECTIVE: The liver hanging maneuver (LHM) is rarely applied in laparoscopic right hepatectomy (LRH) because of the difficulty encountered in retrohepatic tunnel (RT) dissection and tape positioning. Thus far no report has detailed how to quickly and easily establish RT for laparoscopic LHM in LRH, nor has employment of the Goldfinger dissector to create a total RT been reported. This study's aim was to evaluate the safety and feasibility of establishing RT for laparoscopic LHM using the Goldfinger dissector in LRH...
September 2016: Journal of Zhejiang University. Science. B
Mu-Zi Li, Jin-Rui Wang, Hong Liu, Xia Wang, Kang Gan, Xiu-Ju Liu, De-Li Niu, Xiao-Qing Song
OBJECTIVE: This study explored the effects of different light curing modes and ethanol-wet bonding on dentin bonding strength and durability. METHODS: A total of 54 molars were randomly divided into three groups: Single Bond 2, Gluma Comfort Bond, and N-Bond. Based on the three light-curing modes and presence or absence of ethanol pretreatment, the samples were assigned to six subgroups: high-light mode, ethanol pretreatment+high-light mode, soft-start mode, ethanol pretreatment+soft-start mode, standard mode, and ethanol pretreatment+standard mode...
September 2016: Journal of Zhejiang University. Science. B
Yun-Zhi Ling, Xiao-Hong Li, Li Yu, Ye Zhang, Qi-Sheng Liang, Xiao-di Yang, Hong-Tao Wang
OBJECTIVE: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro. METHODS: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified...
September 2016: Journal of Zhejiang University. Science. B
Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables...
September 2016: Journal of Zhejiang University. Science. B
Jian-Zhen Shan, Yan-Yan Xuan, Qi Zhang, Jian-Jin Huang
OBJECTIVE: To explore the efficacy of ursolic acid in sensitizing colon cancer cells to chemotherapy under hypoxia and its underlying mechanisms. METHODS: Three colon cancer cell lines (RKO, LoVo, and SW480) were used as in vitro models. 5-Fluorouracil (5-FU) and oxaliplatin were used as chemotherapeutic drugs. Cell viability and apoptosis were tested to evaluate the sensitivity of colon cancer cells to chemotherapy. The transcription and expression levels of hypoxia-inducible factor-1α (HIF-1α), multidrug resistance gene 1 (MDR1), and vascular endothelial growth factors (VEGF) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblotting...
September 2016: Journal of Zhejiang University. Science. B
Jing Zhao, Shi-Wei Li, Qian-Qian Gong, Ling-Cui Ding, Ye-Cheng Jin, Jian Zhang, Jian-Gang Gao, Xiao-Yang Sun
It is widely accepted that body weight and adipose mass are tightly regulated by homeostatic mechanisms, in which leptin plays a critical role through hypothalamic pathways, and obesity is a result of homeostatic disorder. However, in C57BL/6J mice, we found that Rcan2 increases food intake and plays an important role in the development of age- and diet-induced obesity through a leptin-independent mechanism. RCAN2 was initially identified as a thyroid hormone (T3)-responsive gene in human fibroblasts. Expression of RCAN2 is regulated by T3 through the PI3K-Akt/PKB-mTOR-Rps6kb1 signaling pathway...
September 2016: Journal of Zhejiang University. Science. B
Shan-Shan Wang, Sovichea Lay, Hai-Ning Yu, Sheng-Rong Shen
A high quality diet is believed to play a functional role in promoting the healthy growth of mankind and preventing many kinds of chronic degenerative diseases, including cancer, cardiovascular disease, diabetes, and obesity. Adherence to a high quality diet has been strongly associated with a lower risk of mortality. To help promote healthy lifestyles and physical strength, the Chinese government has produced a new revised version of the Dietary Guidelines for Chinese Residents (2016) and the Chinese Food Pagoda, as guidance for dietary intake among its population...
September 2016: Journal of Zhejiang University. Science. B
Xiao-Hong Pan, Amina Mahemuti, Xue-Hua Zhang, Ya-Ping Wang, Po Hu, Ju-Bo Jiang, Mei-Xiang Xiang, Gang Liu, Jian-An Wang
OBJECTIVE: Studies have demonstrated that Tai Chi exercise improves blood lipid level with inconsistent results. A meta-analysis was conducted to quantify the effects of Tai Chi on blood lipid profiles in humans. METHODS: We screened the databases of PubMed, EMBASE, Cochrane Library (Central), Web of Science, China National Knowledge Infrastructure (CNKI), Wanfang data, and for randomized controlled trials with Physiotherapy Evidence Database (PEDro) score more than 3 points up to June 2015...
August 2016: Journal of Zhejiang University. Science. B
Fa-Chun Guan, Zhi-Peng Sha, Yu-Yang Zhang, Jun-Feng Wang, Chao Wang
Home courtyard agriculture is an important model of agricultural production on the Tibetan plateau. Because of the sensitive and fragile plateau environment, it needs to have optimal performance characteristics, including high sustainability, low environmental pressure, and high economic benefit. Emergy analysis is a promising tool for evaluation of the environmental-economic performance of these production systems. In this study, emergy analysis was used to evaluate three courtyard agricultural production models: Raising Geese in Corn Fields (RGICF), Conventional Corn Planting (CCP), and Pea-Wheat Rotation (PWR)...
August 2016: Journal of Zhejiang University. Science. B
Ya-Qian Li, Kai Song, Ya-Chai Li, Jie Chen
Trichoderma-based formulations are applied as commercial biocontrol agents for soil-borne plant pathogens. Chlamydospores are active propagules in Trichoderma spp., but their production is currently limited due to a lack of optimal liquid fermentation technology. In this study, we explored response surface methodologies for optimizing fermentation technology in Trichoderma SH2303. Our initial studies, using the Plackett-Burman design, identified cornmeal, glycerol, and initial pH levels as the most significant factors (P<0...
August 2016: Journal of Zhejiang University. Science. B
Cai-Fang Wen, Li-Xin Xi, Shan Zhao, Zhong-Xiang Hao, Lu Luo, Hong Liao, Zhen-Rong Chen, Rong She, Guo-Quan Han, San-Jie Cao, Rui Wu, Qi-Gui Yan, Rong Hou
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1(T), was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1(T) grew optimally at pH 7.0-8.0, at 28-30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1(T) belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81(T) (96...
August 2016: Journal of Zhejiang University. Science. B
Ramila Azat, Yan Liu, Wei Li, Abdurihim Kayir, Ding-Bo Lin, Wen-Wen Zhou, Xiao-Dong Zheng
Six lactic acid bacterial (LAB) strains were isolated from traditionally fermented Xinjiang cheese and evaluated for functional and probiotic properties and potentials as starter cultures. The isolated six LAB strains comprised Lactobacillus rhamnosus (one strain), Lactobacillus helveticus (one strain), and Enterococcus hirae (four strains). All of the six strains were tolerant to acidic and bile salt conditions. Among which, the L. rhamnosus R4 strain showed more desirable antimicrobial, auto-aggregation, and hydrophobic activity...
August 2016: Journal of Zhejiang University. Science. B
Yue-Jin Pu, Yan Wu, Xiao-Juan Xu, Jin-Ping Du, Yan-Zhang Gong
The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
August 2016: Journal of Zhejiang University. Science. B
Sabuj Kanti Mazumder, Simon Kumar Das, Yosni Bakar, Mazlan Abd Ghaffar
In this study we aimed to analyze the effects of water temperature and diet on the length-weight relationship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures (22, 26, 30, and 34 °C) and two diets (commercial pellet and natural shrimp). Fish were fed twice daily. L. malabaricus exhibited negative allometric growth (b<3) at the beginning of the experiment (Day 0) at all temperatures and both diets except for 22 °C fed with shrimp, which showed isometric growth (b=3)...
August 2016: Journal of Zhejiang University. Science. B
Ping Li, Xuan Li, Qing Gu, Xiu-Yu Lou, Xiao-Mei Zhang, Da-Feng Song, Chen Zhang
OBJECTIVE: In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. METHODS: The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank...
August 2016: Journal of Zhejiang University. Science. B
Xi-Ming Li, Feng Li, Zhong-Kai Liu, Ming-Tao Shao
The original version of this article unfortunately contained a mistake. In "Abstract" and the 1st para-graph of Section 2.1, the full name of the abbreviation "ASA" was incorrect in "American Standards Association (ASA)". The correct version should be "American Society of Anesthesiologists (ASA)".
July 2016: Journal of Zhejiang University. Science. B
Wolfgang Huber, Veit Phillip, Josef Höllthaler, Caroline Schultheiss, Bernd Saugel, Roland M Schmid
OBJECTIVE: Comparison of global end-diastolic volume index (GEDVI) obtained by femoral and jugular transpulmonary thermodilution (TPTD) indicator injections using the EV1000/VolumnView(®) device (Edwards Lifesciences, Irvine, USA). METHODS: In an 87-year-old woman with hypovolemic shock and equipped with both jugular and femoral vein access and monitored with the EV1000/VolumeView(®) device, we recorded 10 datasets, each comprising duplicate TPTD via femoral access and duplicate TPTD (20 ml cold saline) via jugular access...
July 2016: Journal of Zhejiang University. Science. B
Xin-Bo Wu, Guo-Xin Fan, Xin Gu, Tu-Gang Shen, Xiao-Fei Guan, An-Nan Hu, Hai-Long Zhang, Shi-Sheng He
OBJECTIVES: This study aimed to compare the learning curves of percutaneous endoscopic lumbar discectomy (PELD) in a transforaminal approach at the L4/5 and L5/S1 levels. METHODS: We retrospectively reviewed the first 60 cases at the L4/5 level (Group I) and the first 60 cases at the L5/S1 level (Group II) of PELD performed by one spine surgeon. The patients were divided into subgroups A, B, and C (Group I: A cases 1-20, B cases 21-40, C cases 41-60; Group II: A cases 1-20, B cases 21-40, C cases 41-60)...
July 2016: Journal of Zhejiang University. Science. B
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