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Journal of Zhejiang University. Science. B

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https://www.readbyqxmd.com/read/28071001/purified-isolation-of-vacuoles-from-sedum-alfredii-leaf-derived-protoplasts
#1
Xiao-Yu Gao, Xing-Cheng Liao, Ruo-Lai Wu, Ting Liu, Hai-Xing Wang, Ling-Li Lu
This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macerozyme at 50 °C for 5 min significantly accelerated the cell wall degradation. For the most optimal conditions for mesophyll protoplast isolation, the mixture of fresh leaves and cell lysates was followed by a 2-h-long vibration. The protoplast lysate for vacuole isolation was diluted, and 0.675 mmol/L was identified as the most appropriate 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonic acid (CHAPS) level, in which S...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28071000/chemical-composition-and-antioxidant-activities-of-essential-oils-from-different-parts-of-the-oregano
#2
Fei Han, Guang-Qiang Ma, Ming Yang, Li Yan, Wei Xiong, Ji-Cheng Shu, Zhi-Dong Zhao, Han-Lin Xu
This research was undertaken in order to characterize the chemical compositions and evaluate the antioxidant activities of essential oils obtained from different parts of the Origanum vulgare L. It is a medicinal plant used in traditional Chinese medicine for the treatment of heat stroke, fever, vomiting, acute gastroenteritis, and respiratory disorders. The chemical compositions of the three essential oils from different parts of the oregano (leaves-flowers, stems, and roots) were identified by gas chromatography-mass spectrometry (GC-MS)...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070999/inhibitory-effect-of-shenqi-fuzheng-injection-combined-with-docetaxel-on-lung-cancer-cells
#3
Bo-Yu Dong, Cheng Wang, Li Tan, Ning Tan, Meng-di Zhao, Yang Lu, Shou-Ying Du
This study investigated the anticancer effect of Shenqi Fuzheng (SQFZ) injection combined with docetaxel on lung cancer cell lines of A549 and Lewis lung cancer (LLC). SQFZ injection alone cannot inhibit the vitality of lung cancer cells, but the antitumor activity of SQFZ combined with docetaxel was significantly higher than that using docetaxel alone.
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070998/successful-treatment-of-accessory-breast-cancer-with-endocrine-therapy
#4
Chun-Xi Wang, Shu-Li Guo, Li-Na Han
Accessory breast cancers in males are extremely rare, and only a few cases have been reported in the literature. In this paper, an 87-year-old male patient was diagnosed with an accessory breast cancer by means of computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography-computed tomography (PET-CT), and immunohistochemistry based on needle biopsy, and has undergone successful resection and postoperative adjuvant endocrine therapy. He was the oldest male patient with an accessory breast cancer reported in the Chinese Hospital Knowledge Database and PubMed literature from 1975 to 2015...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070997/involvement-of-endothelial-progenitor-cells-in-the-formation-of-plexiform-lesions-in-broiler-chickens-possible-role-of-local-immune-inflammatory-response
#5
Xun Tan, Fan-Guo Juan, Ali Q Shah
Plexiform lesions (PLs), which are often accompanied by perivascular infiltrates of mononuclear cells, represent the hallmark lesions of pulmonary arteries in humans suffering from severe pulmonary arterial hypertension (PAH). Endothelial progenitor cells (EPCs) have been recently implicated in the formation of PLs in human patients. PLs rarely develop in rodent animal models of PAH but can develop spontaneously in broiler chickens. The aim of the present study was to confirm the presence of EPCs in the PLs in broilers...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070996/distribution-and-differentiation-of-myeloid-derived-suppressor-cells-after-fluid-resuscitation-in-mice-with-hemorrhagic-shock
#6
Jiu-Kun Jiang, Wen Fang, Liang-Jie Hong, Yuan-Qiang Lu
OBJECTIVE: To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES). METHODS: BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs)...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070995/haplotype-of-platelet-receptor-p2ry12-gene-is-associated-with-residual-clopidogrel-on-treatment-platelet-reactivity
#7
Xiao-Yan Nie, Jun-Lei Li, Yong Zhang, Yang Xu, Xue-Li Yang, Yu Fu, Guang-Kai Liang, Yun Lu, Jian Liu, Lu-Wen Shi
OBJECTIVE: To investigate a possible association between common variations of the P2RY12 and the residual clopidogrel on-treatment platelet reactivity after adjusting for the influence of CYP2C19 tested by thromboelastography (TEG). METHODS: One hundred and eighty patients with acute coronary syndrome (ACS) treated with clopidogrel and aspirin were included and platelet function was assessed by TEG. Five selected P2RY12 single nucleotide polymorphisms (SNPs; rs6798347, rs6787801, rs6801273, rs6785930, and rs2046934), which cover the common variations in the P2RY12 gene and its regulatory regions, and three CYP2C19 SNPs ((*)2,(*)3,(*)17) were genotyped and possible haplotypes were analyzed...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070994/involvement-of-microrna-718-a-new-regulator-of-egr3-in-regulation-of-malignant-phenotype-of-hcc-cells
#8
Zhong-Dong Wang, Fan-Yong Qu, Yuan-Yuan Chen, Zhang-Shen Ran, Hai-Yan Liu, Hai-Dong Zhang
OBJECTIVE: Hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. MicroRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070993/use-of-liposomal-doxorubicin-for-adjuvant-chemotherapy-of-breast-cancer-in-clinical-practice
#9
Ming Zhao, Xian-Feng Ding, Jian-Yu Shen, Xi-Ping Zhang, Xiao-Wen Ding, Bin Xu
Breast cancer is one of the malignant tumors with the highest morbidity and mortality. It is helpful to reduce the rate of tumor recurrence and metastasis by treating breast cancer with adjuvant chemotherapy, so as to increase the cure rate or survival of patients. In recent years, liposomes have been regarded as a kind of new carrier for targeted drugs. Being effective for enhancing drug efficacy and reducing side effects, they have been widely used for developing anticancer drugs. As a kind of anthracycline with high anticancer activity, doxorubicin can treat or alleviate a variety of malignant tumors effectively when it is used on its own or in combination with other anticancer drugs...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070992/molecular-signal-networks-and-regulating-mechanisms-of-the-unfolded-protein-response
#10
Jing Gong, Xing-Zhi Wang, Tao Wang, Jiao-Jiao Chen, Xiao-Yuan Xie, Hui Hu, Fang Yu, Hui-Lin Liu, Xing-Yan Jiang, Han-Dong Fan
Within the cell, several mechanisms exist to maintain homeostasis of the endoplasmic reticulum (ER). One of the primary mechanisms is the unfolded protein response (UPR). In this review, we primarily focus on the latest signal webs and regulation mechanisms of the UPR. The relationships among ER stress, apoptosis, and cancer are also discussed. Under the normal state, binding immunoglobulin protein (BiP) interacts with the three sensors (protein kinase RNA-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1α (IRE1α))...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604865/maternal-high-fat-diet-inversely-affects-insulin-sensitivity-in-dams-and-young-adult-male-rat-offspring
#11
Roxana Karbaschi, Forouzan Sadeghimahalli, Homeira Zardooz
This study attempts to further clarify the potential effects of maternal high-fat (HF) diet on glucose homeostasis in dams and young adult male rat offspring. Female rats were divided into control (CON dams) and HF (HF dams) diet groups, which received the diet 4 weeks prior to and through pregnancy and lactation periods. Blood samples were taken to determine metabolic parameters, then an intraperitoneal glucose tolerance test (IPGTT) was performed. Maternal HF diet increased intra-abdominal fat mass and plasma corticosterone level, but decreased leptin concentration in dams...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604864/high-pressure-balloon-dilation-for-male-anterior-urethral-stricture-single-center-experience
#12
Shi-Cheng Yu, Hai-Yang Wu, Wei Wang, Li-Wei Xu, Guo-Qing Ding, Zhi-Gen Zhang, Gong-Hui Li
OBJECTIVES: We retrospectively reviewed the urethral stricture cases treated in our tertiary center, and assessed the safety and feasibility of the high-pressure balloon dilation (HPBD) technique for anterior urethral stricture. METHODS: From January 2009 to December 2012, a total of 31 patients with anterior urethral strictures underwent HPBD at our center, while another 25 cases were treated by direct vision internal urethrotomy (DVIU). Patient demographics, stricture characteristics, surgical techniques, and operative outcomes were assessed and compared between the two groups...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604863/can-retrohepatic-tunnel-be-quickly-and-easily-established-for-laparoscopic-liver-hanging-maneuver-by-goldfinger-dissector-in-laparoscopic-right-hepatectomy
#13
Liu-Xin Cai, Fang-Qiang Wei, Yi-Chen Yu, Xiu-Jun Cai
OBJECTIVE: The liver hanging maneuver (LHM) is rarely applied in laparoscopic right hepatectomy (LRH) because of the difficulty encountered in retrohepatic tunnel (RT) dissection and tape positioning. Thus far no report has detailed how to quickly and easily establish RT for laparoscopic LHM in LRH, nor has employment of the Goldfinger dissector to create a total RT been reported. This study's aim was to evaluate the safety and feasibility of establishing RT for laparoscopic LHM using the Goldfinger dissector in LRH...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604862/effects-of-light-curing-modes-and-ethanol-wet-bonding-on-dentin-bonding-properties
#14
Mu-Zi Li, Jin-Rui Wang, Hong Liu, Xia Wang, Kang Gan, Xiu-Ju Liu, De-Li Niu, Xiao-Qing Song
OBJECTIVE: This study explored the effects of different light curing modes and ethanol-wet bonding on dentin bonding strength and durability. METHODS: A total of 54 molars were randomly divided into three groups: Single Bond 2, Gluma Comfort Bond, and N-Bond. Based on the three light-curing modes and presence or absence of ethanol pretreatment, the samples were assigned to six subgroups: high-light mode, ethanol pretreatment+high-light mode, soft-start mode, ethanol pretreatment+soft-start mode, standard mode, and ethanol pretreatment+standard mode...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604861/protective-effects-of-parecoxib-on-rat-primary-astrocytes-from-oxidative-stress-induced-by-hydrogen-peroxide
#15
Yun-Zhi Ling, Xiao-Hong Li, Li Yu, Ye Zhang, Qi-Sheng Liang, Xiao-di Yang, Hong-Tao Wang
OBJECTIVE: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro. METHODS: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604860/a-comparative-study-of-conventional-and-supercritical-fluid-extraction-methods-for-the-recovery-of-secondary-metabolites-from-syzygium-campanulatum-korth
#16
Abdul Hakeem Memon, Mohammad Shahrul Ridzuan Hamil, Madeeha Laghari, Fahim Rithwan, Salman Zhari, Mohammed Ali Ahmed Saeed, Zhari Ismail, Amin Malik Shah Abdul Majid
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604859/ursolic-acid-sensitized-colon-cancer-cells-to-chemotherapy-under-hypoxia-by-inhibiting-mdr1-through-hif-1%C3%AE
#17
Jian-Zhen Shan, Yan-Yan Xuan, Qi Zhang, Jian-Jin Huang
OBJECTIVE: To explore the efficacy of ursolic acid in sensitizing colon cancer cells to chemotherapy under hypoxia and its underlying mechanisms. METHODS: Three colon cancer cell lines (RKO, LoVo, and SW480) were used as in vitro models. 5-Fluorouracil (5-FU) and oxaliplatin were used as chemotherapeutic drugs. Cell viability and apoptosis were tested to evaluate the sensitivity of colon cancer cells to chemotherapy. The transcription and expression levels of hypoxia-inducible factor-1α (HIF-1α), multidrug resistance gene 1 (MDR1), and vascular endothelial growth factors (VEGF) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblotting...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604858/a-disputed-evidence-on-obesity-comparison-of-the-effects-of-rcan2-and-rps6kb1-mutations-on-growth-and-body-weight-in-c57bl-6j-mice
#18
Jing Zhao, Shi-Wei Li, Qian-Qian Gong, Ling-Cui Ding, Ye-Cheng Jin, Jian Zhang, Jian-Gang Gao, Xiao-Yang Sun
It is widely accepted that body weight and adipose mass are tightly regulated by homeostatic mechanisms, in which leptin plays a critical role through hypothalamic pathways, and obesity is a result of homeostatic disorder. However, in C57BL/6J mice, we found that Rcan2 increases food intake and plays an important role in the development of age- and diet-induced obesity through a leptin-independent mechanism. RCAN2 was initially identified as a thyroid hormone (T3)-responsive gene in human fibroblasts. Expression of RCAN2 is regulated by T3 through the PI3K-Akt/PKB-mTOR-Rps6kb1 signaling pathway...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27604857/dietary-guidelines-for-chinese-residents-2016-comments-and-comparisons
#19
Shan-Shan Wang, Sovichea Lay, Hai-Ning Yu, Sheng-Rong Shen
A high quality diet is believed to play a functional role in promoting the healthy growth of mankind and preventing many kinds of chronic degenerative diseases, including cancer, cardiovascular disease, diabetes, and obesity. Adherence to a high quality diet has been strongly associated with a lower risk of mortality. To help promote healthy lifestyles and physical strength, the Chinese government has produced a new revised version of the Dietary Guidelines for Chinese Residents (2016) and the Chinese Food Pagoda, as guidance for dietary intake among its population...
September 2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27487809/effect-of-tai-chi-exercise-on-blood-lipid-profiles-a-meta-analysis-of-randomized-controlled-trials
#20
Xiao-Hong Pan, Amina Mahemuti, Xue-Hua Zhang, Ya-Ping Wang, Po Hu, Ju-Bo Jiang, Mei-Xiang Xiang, Gang Liu, Jian-An Wang
OBJECTIVE: Studies have demonstrated that Tai Chi exercise improves blood lipid level with inconsistent results. A meta-analysis was conducted to quantify the effects of Tai Chi on blood lipid profiles in humans. METHODS: We screened the databases of PubMed, EMBASE, Cochrane Library (Central), Web of Science, China National Knowledge Infrastructure (CNKI), Wanfang data, and Clinicaltrials.gov for randomized controlled trials with Physiotherapy Evidence Database (PEDro) score more than 3 points up to June 2015...
August 2016: Journal of Zhejiang University. Science. B
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