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Journal of Zhejiang University. Science. B

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https://www.readbyqxmd.com/read/28471119/recurrence-of-non-cardiogenic-pulmonary-edema-and-sustained-hypotension-shock-in-cystic-pheochromocytoma
#1
Jin Dai, Shen-Jie Chen, Bing-Sheng Yang, Shu-Min Lü, Min Zhu, Yi-Fei Xu, Jie Chen, Hong-Wen Cai, Wei Mao
Pheochromocytoma is a rare neuroendocrine tumor which derives from chromaffin cells of the adrenal gland or relevant to sympathetic nerves and ganglia. The clinical features of pheochromocytoma are various. Paroxysmal episodes of serious hypertension, headache, palpitation, and diaphoresis are the typical manifestations (Bravo, 2004). Hypotension shock, pulmonary edema, and acute coronary syndrome induced by pheochromocytoma are uncommon (Malindretos et al., 2008; Batisse-Lignier et al., 2015). In this study, we present a rare case of cystic pheochromocytoma causing recurrent hypotension shock, non-cardiogenic pulmonary edema, and acute coronary syndrome, and the possible mechanisms are discussed...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471118/ginsenoside-rg1-promotes-neural-differentiation-of-mouse-adipose-derived-stem-cells-via-the-mirna-124-signaling-pathway
#2
Juan Dong, Guo Zhu, Tian-Cheng Wang, Fu-Shan Shi
We have explored the role of ginsenoside Rg1 in promoting the differentiation of mouse adipose-derived stem cells (mADSC) towards the neuronal lineage. The central nervous system has long been regarded as incapable of self-repair; therefore neuronal differentiation from stem cells is of great interest. However, the use of embryonic stem cells is limited due to their inaccessibility and for ethical reasons, so the search is on for alternative pluripotent cells capable of differentiating into neuronal cells. Adipose-derived stem cells (ADSC) can differentiate into different cell types, including neuronal cells: their accessibility, low risk, and capacity for long-term growth and self-renewal have made them the preferred stem cell type for clinical applications...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471117/antepartum-hemorrhage-from-previous-cesarean-sectioned-uterus-as-a-potential-sign-of-uterine-artery-pseudoaneurysm
#3
Ning Zhang, Wei-Hua Lou, Xue-Bin Zhang, Jian-Hua Lin, Wen Di
Postpartum hemorrhage (PPH), a leading cause of maternal mortality, can occur within 24 h of delivery (primary PPH), or during the period from 24 h after delivery to Week 6 of puerperium (secondary PPH). It requires health professionals to be alert to the symptoms to ensure prompt diagnosis and treatment, especially in the case of rupture of a uterine artery pseudoaneurysm (UAP) due to its life-threatening consequence (Baba et al., 2014). Most of the published case reports or case serials describe UAP as a possible cause of delayed PPH after traumatic procedures during delivery or pregnancy termination, including cesarean section (CS), manual removal of the placenta, or dilation and curettage (D&C) (Wald, 2003)...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471116/no-evidence-of-seed-transmissibility-of-tomato-yellow-leaf-curl-virus-in-nicotiana-benthamiana
#4
Tábata Rosas-Díaz, Dan Zhang, Rosa Lozano-Durán
Recent work reported that Tomato yellow leaf curl virus (TYLCV) is seed-transmissible in tomato, contrary to previous belief. In this work, we explore whether TYLCV is also a seed-borne virus in another member of the Solanaceae family, the experimental host Nicotiana benthamiana.
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471115/clinical-characteristics-and-treatment-of-thyroid-cancer-in-children-and-adolescents-a-retrospective-analysis-of-83-patients
#5
Xiao-Chun Mao, Wen-Qiao Yu, Jin-Biao Shang, Ke-Jing Wang
OBJECTIVE: To study the clinical characteristics, treatment, and prognosis of thyroid cancer in children and adolescents. METHODS: We performed a retrospective analysis of clinical data from 83 cases of thyroid cancer in children and adolescents from January 1990 to December 2010. We compared extra-thyroid extension, lymph node metastasis, distant metastasis, and prognosis between pediatric patients ≤12 years of age (27 cases) and those >12 years of age (56 cases)...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471114/phenotype-genotype-correlation-with-sanger-sequencing-identified-retinol-dehydrogenase-12-rdh12-compound-heterozygous-variants-in-a-chinese-family-with-leber-congenital-amaurosis
#6
Yun Li, Qing Pan, Yang-Shun Gu
BACKGROUND: Leber congenital amaurosis (LCA) is a group of clinically and genetically heterogeneous retinal dystrophy. To date, 22 genes are known to be responsible for LCA, and some specific phenotypic features could provide significant prognostic information for a potential genetic etiology. This study is to identify gene variants responsible for LCA in a Chinese family using direct Sanger sequencing, with the help of phenotype-genotype correlations. METHODS: A Chinese family with six members including two individuals affected with LCA was studied...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471113/antibacterial-mechanism-of-high-mobility-group-nucleosomal-binding-domain-2-on-the-gram-negative-bacteria-escherichia-coli
#7
Heng Li, Xiao-Fei Shen, Xin-E Zhou, Yan-E Shi, Lu-Xia Deng, Yi Ma, Xiao-Ying Wang, Jing-Yu Li, Ning Huang
OBJECTIVE: To investigate the antibacterial mechanism of high-mobility group nucleosomal-binding domain 2 (HMGN2) on Escherichia coli K12, focusing on the antibacterial and antibiofilm formation effects. Its chemotactic activity on human neutrophils was also investigated. METHODS: Human tissue-derived HMGN2 (tHMGN2) was extracted from fresh uterus fiber cystadenoma and purified by HP1100 reversed-phase high-performance liquid chromatography (RP-HPLC). Recombinant human HMGN2 (rHMGN2) was generated in E...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471112/immune-recovery-after-fluid-resuscitation-in-rats-with-severe-hemorrhagic-shock
#8
Feng Yao, Yuan-Qiang Lu, Jiu-Kun Jiang, Lin-Hui Gu, Han-Zhou Mou
OBJECTIVE: To investigate the effects of resuscitation with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES) on regulatory T cells (Tregs), helper T 1 (Th1)/Th2 and cytotoxic T 1 (Tc1)/Tc2 profiles in the treatment of hemorrhagic shock. METHODS: Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS (n=8), HTS (n=8), or HES (n=8); sham (n=8) and naive control (n=8) groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471111/intelligent-diagnosis-of-jaundice-with-dynamic-uncertain-causality-graph-model
#9
Shao-Rui Hao, Shi-Chao Geng, Lin-Xiao Fan, Jia-Jia Chen, Qin Zhang, Lan-Juan Li
Jaundice is a common and complex clinical symptom potentially occurring in hepatology, general surgery, pediatrics, infectious diseases, gynecology, and obstetrics, and it is fairly difficult to distinguish the cause of jaundice in clinical practice, especially for general practitioners in less developed regions. With collaboration between physicians and artificial intelligence engineers, a comprehensive knowledge base relevant to jaundice was created based on demographic information, symptoms, physical signs, laboratory tests, imaging diagnosis, medical histories, and risk factors...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471110/rapid-quantification-of-multi-components-in-alcohol-precipitation-liquid-of-codonopsis-radix-using-near-infrared-spectroscopy-nirs
#10
Yu Luo, Wen-Long Li, Wen-Hua Huang, Xue-Hua Liu, Yan-Gang Song, Hai-Bin Qu
A near infrared spectroscopy (NIRS) approach was established for quality control of the alcohol precipitation liquid in the manufacture of Codonopsis Radix. By applying NIRS with multivariate analysis, it was possible to build variation into the calibration sample set, and the Plackett-Burman design, Box-Behnken design, and a concentrating-diluting method were used to obtain the sample set covered with sufficient fluctuation of process parameters and extended concentration information. NIR data were calibrated to predict the four quality indicators using partial least squares regression (PLSR)...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471109/ethaselen-a-novel-organoselenium-anticancer-agent-targeting-thioredoxin-reductase-1-reverses-cisplatin-resistance-in-drug-resistant-k562-cells-by-inducing-apoptosis
#11
Suo-Fu Ye, Yong Yang, Lin Wu, Wei-Wei Ma, Hui-Hui Zeng
It has been reported that Ethaselen shows inhibitory effects on thioredoxin reductase (TrxR) activity and human tumor cell growth. In order to find an efficient way to reverse cisplatin resistance, we investigated the reversal effects of Ethaselen on cisplatin resistance in K562/cisplatin (CDDP) cells that were established by pulse-inducing human erythrocyte leukemic cell line K562, which are fivefold more resistant to cisplatin compared to K562 cells. The morphology and growth showed that the adhesion of K562/CDDP further decreased while the cell volume increased...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471108/dna-methylation-in-the-tumor-microenvironment
#12
Meng-Wen Zhang, Kenji Fujiwara, Xu Che, Shu Zheng, Lei Zheng
The tumor microenvironment (TME) plays an important role in supporting cancer progression. The TME is composed of tumor cells, the surrounding tumor-associated stromal cells, and the extracellular matrix (ECM). Crosstalk between the TME components contributes to tumorigenesis. Recently, one of our studies showed that pancreatic ductal adenocarcinoma (PDAC) cells can induce DNA methylation in cancer-associated fibroblasts (CAFs), thereby modifying tumor-stromal interactions in the TME, and subsequently creating a TME that supports tumor growth...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378574/efficacy-of-vaccination-and-nisin-z-treatments-to-eliminate-intramammary-staphylococcus-aureus-infection-in-lactating-cows
#13
Ran Guan, Jun-Qiang Wu, Wei Xu, Xiao-Yan Su, Song-Hua Hu
This study evaluated the effect of a Staphylococcus aureus bacterin and nisin on bovine subclinical mastitis. A total of 75 Holstein subclinically mastitic cows were randomly allocated to three groups with 25 cows per group. In group I, an intramammary infusion of nisin Z at a dose of 2.50×10(6) IU was carried out once daily for three days, and an autogenous S. aureus bacterin was inoculated into the supramammary lymph node one week before and one week after nisin treatment. In group II, nisin was administered in the same way as in group I, but no bacterin was inoculated...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378573/bispectral-index-guided-sedation-in-transfemoral-transcatheter-aortic-valve-implantation-a-retrospective-control-study
#14
Wei He, Rong-Rong Huang, Qing-Yu Shi, Xian-Bao Liu, Jian-An Wang, Min Yan
OBJECTIVE: Transcatheter aortic valve implantation (TAVI) is a minimally invasive therapy for elderly patients with severe aortic valve stenosis who were refused surgical aortic valve replacement because of the high perioperative risk. Traditionally, this procedure has been done under general anesthesia, but more recently local anesthesia and sedation have become popular. This research assessed the effectiveness of transfemoral TAVI under bispectral index (BIS)-guided sedation. METHODS: In this single-center retrospective control analysis, clinical data, including demographic characteristics, echocardiography, periprocedural data, and main complications, were collected and assessed in 113 patients undergoing TAVI through the femoral artery under general anesthesia (GA group, n=36) and under BIS-guided sedation (SED group, n=77)...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378572/effect-of-compatible-herbs-on-the-pharmacokinetics-of-effective-components-of-panax-notoginseng-in-fufang-xueshuantong-capsule
#15
Huan-Huan Pang, Meng-Yi Li, Yuan Wang, Min-Ke Tang, Chang-Hua Ma, Jian-Mei Huang
Fufang Xueshuantong (FXT) is a well-known Chinese herbal formula which has been used to treat cardiovascular and ophthalmic diseases, especially diabetic retinopathy. Panax notoginseng (Burkill) F.H. Chen (PN) is the main herb of FXT, whose major bioactive constituents are ginsenosides. However, the scientific basis of the compatibility of FXT is still ambiguous. The present study investigated the scientific basis of the compatibility of FXT by comparing the pharmacokinetics of marker compounds after oral administrations of PN and FXT...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378571/protein-tyrosine-phosphatase-1b-expression-contributes-to-the-development-of-breast-cancer
#16
Shi-Chong Liao, Jin-Xin Li, Li Yu, Sheng-Rong Sun
The protein tyrosine phosphatase 1B (PTP1B) is an important regulator of metabolism. The relationship between PTP1B and tumors is quite complex. The purpose of this study is to explore the expression pattern and role of PTP1B in breast cancer. The expression of PTP1B was detected in 67 samples of breast cancer tissue by Western blot. Cell growth assay, Transwell migration assay, and Scratch motility assay were used to examine the proliferation and migration of MCF-7 with and without PTP1B. The total levels and phosphorylated levels of signal transduction and activator of transcription 3 (STAT3) and the expression of C-C motif chemokine ligand 5 (CCL5) were also examined by Western blot...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378570/evaluation-of-the-antioxidant-property-and-effects-in-caenorhabditis-elegans-of-xiangxi-flavor-vinegar-a-hunan-local-traditional-vinegar
#17
Run-Ting Huang, Qing Huang, Gen-Liang Wu, Chun-Guang Chen, Zong-Jun Li
Xiangxi flavor vinegar (XV) is one of Hunan Province's traditional fermented vinegars. It is produced from herb, rice, and spring water with spontaneous liquid-state fermentation techniques. In this study, we investigated the antioxidant property of XV by analyzing its antioxidant compounds, its free radical scavenging property in vitro and in vivo, and its effects on antioxidant enzyme activity and apoptosis in Caenorhabditis elegans. The results showed that XV is rich in antioxidants. In particular, ligustrazine reached 6...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378569/porcine-circovirus-type-2-capsid-protein-induces-unfolded-protein-response-with-subsequent-activation-of-apoptosis
#18
Ying-Shan Zhou, Yuan-Xing Gu, Bao-Zhu Qi, Yi-Kai Zhang, Xiao-Liang Li, Wei-Huan Fang
Porcine circovirus type 2 (PCV2) has recently been reported to elicit the unfolded protein response (UPR) via activation of the PERK/eIF2α (RNA-activated protein kinase-like endoplasmic reticulum (ER) kinase/eukaryotic initiation factor 2α) pathway. This study attempted to examine which viral protein might be involved in inducing UPR and whether this cellular event would lead to apoptosis of the cells expressing the viral protein. By transient expression, we found that both replicase (Rep) and capsid (Cap) proteins of PCV2 could induce ER stress as shown by increased phosphorylation of PERK with subsequent activation of the eIF2α-ATF4 (activating transcription factor 4)-CHOP (CCAAT/enhancer-binding protein homologous protein) axis...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378568/rapid-prototyping-technology-and-its-application-in-bone-tissue-engineering
#19
Bo Yuan, Sheng-Yuan Zhou, Xiong-Sheng Chen
Bone defects arising from a variety of reasons cannot be treated effectively without bone tissue reconstruction. Autografts and allografts have been used in clinical application for some time, but they have disadvantages. With the inherent drawback in the precision and reproducibility of conventional scaffold fabrication techniques, the results of bone surgery may not be ideal. This is despite the introduction of bone tissue engineering which provides a powerful approach for bone repair. Rapid prototyping technologies have emerged as an alternative and have been widely used in bone tissue engineering, enhancing bone tissue regeneration in terms of mechanical strength, pore geometry, and bioactive factors, and overcoming some of the disadvantages of conventional technologies...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28378567/insights-into-the-microbial-diversity-and-community-dynamics-of-chinese-traditional-fermented-foods-from-using-high-throughput-sequencing-approaches
#20
Guo-Qing He, Tong-Jie Liu, Faizan A Sadiq, Jing-Si Gu, Guo-Hua Zhang
Chinese traditional fermented foods have a very long history dating back thousands of years and have become an indispensable part of Chinese dietary culture. A plethora of research has been conducted to unravel the composition and dynamics of microbial consortia associated with Chinese traditional fermented foods using culture-dependent as well as culture-independent methods, like different high-throughput sequencing (HTS) techniques. These HTS techniques enable us to understand the relationship between a food product and its microbes to a greater extent than ever before...
2017: Journal of Zhejiang University. Science. B
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