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Journal of Zhejiang University. Science. B

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https://www.readbyqxmd.com/read/28585432/full-genome-analysis-of-swine-genotype-3-hepatitis-e-virus-isolated-from-eastern-china
#1
Jiang-Bing Shuai, Lu-Huan Li, Ai-Yun Li, Yong-Qiang He, Xiao-Feng Zhang
Hepatitis E is believed to occur in both endemic and sporadic forms in developing countries, which causes a major public health problem in Asia and Africa (Meng, 2010; Wang et al., 2016a). Recent studies have documented that the disease is also endemic in many industrialized countries (Wenzel et al., 2011). The causative agent, hepatitis E virus (HEV), belonging to the genus Orthohepevirus, is a non-enveloped RNA virus with a single-stranded, positive-sense genome of approximately 7.2 kb (Smith et al., 2014)...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585431/sensory-quality-evaluation-for-appearance-of-needle-shaped-green-tea-based-on-computer-vision-and-nonlinear-tools
#2
Chun-Wang Dong, Hong-Kai Zhu, Jie-Wen Zhao, Yong-Wen Jiang, Hai-Bo Yuan, Quan-Sheng Chen
Tea is one of the three greatest beverages in the world. In China, green tea has the largest consumption, and needle-shaped green tea, such as Maofeng tea and Sparrow Tongue tea, accounts for more than 40% of green tea (Zhu et al., 2017). The appearance of green tea is one of the important indexes during the evaluation of green tea quality. Especially in market transactions, the price of tea is usually determined by its appearance (Zhou et al., 2012). Human sensory evaluation is usually conducted by experts, and is also easily affected by various factors such as light, experience, psychological and visual factors...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585430/satisfactory-clinical-outcome-following-delayed-repositioning-of-a-traumatic-post-lasik-flap-with-dislocation-and-shrinkage-managed-by-irrigation-stretching-and-debridement
#3
Ye-Sheng Xu, Wen-Jia Xie, Yu-Feng Yao
OBJECTIVE: To report surgical management and favorable outcome in a case with delayed repair of traumatic laser in situ keratomileusis (LASIK) flap dislocation with shrinkage and folds. METHODS: A 30-year-old man with a five-year history of bilateral LASIK experienced blunt trauma to his right eye followed by decreased vision for 5 weeks. The surgical management included initially softening the flap by irrigation with balanced salt solution (BSS). The shrinkage folds were carefully and gently stretched by scraping with a 26-gauge cannula accompanied by BSS irrigation...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585429/laparoscopic-versus-open-distal-pancreatectomy-for-pancreatic-ductal-adenocarcinoma-a-single-center-experience
#4
Ai-Bin Zhang, Ye Wang, Chen Hu, Yan Shen, Shu-Sen Zheng
OBJECTIVE: The aim of this study was to compare complications and oncologic outcomes of patients undergoing laparoscopic distal pancreatectomy (LDP) and open distal pancreatectomy (ODP) at a single center. METHODS: Distal pancreatectomies performed for pancreatic ductal adenocarcinoma during a 4-year period were included in this study. A retrospective analysis of a database of this cohort was conducted. RESULTS: Twenty-two patients underwent LDP for pancreatic ductal adenocarcinoma, in comparison to seventy-six patients with comparable tumor characteristics treated by ODP...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585428/anti-hepatocarcinoma-activity-of-tt-1-an-analog-of-melittin-combined-with-interferon-%C3%AE-via-promoting-the-interaction-of-nkg2d-and-mica
#5
Lan-Lan Wan, Da-Qi Zhang, Jin-Nan Zhang, Li-Qun Ren
Hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality. Immunotherapy has emerged in clinical treatment, owing to the limitation and severe side effects of chemotherapy. In the immune system, natural killer (NK) cells are important effectors required to eliminate malignant tumor cells without the limitation of major histocompatibility complex (MHC) molecule issues. Hence, treatment which could stimulate NK cells is of great interest. Here, we investigated the efficacy of the combined therapy of TT-1 (a mutant of melittin) and interferon-α (IFN-α) on NK cells and human liver cancer HepG-2/Huh7 cells in vitro and in vivo, as well as the mechanism involved...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585427/uptake-transport-and-distribution-of-molybdenum-in-two-oilseed-rape-brassica-napus-l-cultivars-under-different-nitrate-ammonium-ratios
#6
Shi-Yu Qin, Xue-Cheng Sun, Cheng-Xiao Hu, Qi-Ling Tan, Xiao-Hu Zhao
OBJECTIVES: To investigate the effects of different nitrate sources on the uptake, transport, and distribution of molybdenum (Mo) between two oilseed rape (Brassica napus L.) cultivars, L0917 and ZS11. METHODS: A hydroponic culture experiment was conducted with four nitrate/ammonium (NO3(-):NH4(+)) ratios (14:1, 9:6, 7.5:7.5, and 1:14) at a constant nitrogen concentration of 15 mmol/L. We examined Mo concentrations in roots, shoots, xylem and phloem sap, and subcellular fractions of leaves to contrast Mo uptake, transport, and subcellular distribution between ZS11 and L0917...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585426/effect-of-helichrysum-plicatum-dc-subsp-plicatum-ethanol-extract-on-gentamicin-induced-nephrotoxicity-in-rats
#7
Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun
The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585425/cide-gene-expression-in-adipose-tissue-liver-and-skeletal-muscle-from-obese-and-lean-pigs
#8
Yue-Qin Qiu, Xue-Fen Yang, Xian-Yong Ma, Yun-Xia Xiong, Zhi-Mei Tian, Qiu-Li Fan, Li Wang, Zong-Yong Jiang
The expression of the cell death-inducing DNA fragmentation factor α-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes' expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585424/punicalagin-protects-bovine-endometrial-epithelial-cells-against-lipopolysaccharide-induced-inflammatory-injury
#9
An Lyu, Jia-Jia Chen, Hui-Chuan Wang, Xiao-Hong Yu, Zhi-Cong Zhang, Ping Gong, Lin-Shu Jiang, Feng-Hua Liu
OBJECTIVE: Bovine endometritis is one of the most common reproductive disorders in cattle. The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and to uncover the underlying mechanisms. METHODS: bEECs were stimulated with different concentrations (1, 10, 30, 50, and 100 μg/ml) of LPS for 3, 6, 9, 12, and 18 h. MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585423/copper-modified-palygorskite-is-effective-in-preventing-and-treating-diarrhea-caused-by-salmonella-typhimurium
#10
Da-Wei Yao, Ze-Zhong Yu, Na Li, Yu-Nong Hou, Jia-Rong Xu, De-Ji Yang
The aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. In this study, the efficacy of copper-modified palygorskite (CM-Pal) in preventing diarrhea caused by Salmonella was specifically examined both in vitro and in vivo. The CM-Pal was prepared with palygorskite (Pal) and copper nitrate. The antibacterial activity of the CM-Pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the CM-Pal to Salmonella typhimurium cultures...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585422/menaquinone-7-production-from-maize-meal-hydrolysate-by-bacillus-isolates-with-diphenylamine-and-analogue-resistance
#11
Jian-Zhong Xu, Wei-Guo Zhang
A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585421/evaluation-of-potential-anti-toxoplasmosis-efficiency-of-combined-traditional-herbs-in-a-mouse-model
#12
Xun-Hui Zhuo, Hong-Chao Sun, Bin Huang, Hai-Jie Yu, Ying Shan, Ai-Fang Du
Toxoplasma gondii is a worldwide spread protozoan and is able to infect almost all warm-blood animals. No effective drugs are available clinically on toxoplasmosis. Chinese traditional herbal medicines have provided remedies for many health problems. There exists a possibility that Chinese herbs may provide protection against T. gondii. This work aims to assess the protective efficacy of combined Chinese herbs against T. gondii. We screened five herbal medicines that have different pharmacological effects and combined them into a prescription according to the traditional Chinese medicine compatibility principle...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471119/recurrence-of-non-cardiogenic-pulmonary-edema-and-sustained-hypotension-shock-in-cystic-pheochromocytoma
#13
Jin Dai, Shen-Jie Chen, Bing-Sheng Yang, Shu-Min Lü, Min Zhu, Yi-Fei Xu, Jie Chen, Hong-Wen Cai, Wei Mao
Pheochromocytoma is a rare neuroendocrine tumor which derives from chromaffin cells of the adrenal gland or relevant to sympathetic nerves and ganglia. The clinical features of pheochromocytoma are various. Paroxysmal episodes of serious hypertension, headache, palpitation, and diaphoresis are the typical manifestations (Bravo, 2004). Hypotension shock, pulmonary edema, and acute coronary syndrome induced by pheochromocytoma are uncommon (Malindretos et al., 2008; Batisse-Lignier et al., 2015). In this study, we present a rare case of cystic pheochromocytoma causing recurrent hypotension shock, non-cardiogenic pulmonary edema, and acute coronary syndrome, and the possible mechanisms are discussed...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471118/ginsenoside-rg1-promotes-neural-differentiation-of-mouse-adipose-derived-stem-cells-via-the-mirna-124-signaling-pathway
#14
Juan Dong, Guo Zhu, Tian-Cheng Wang, Fu-Shan Shi
We have explored the role of ginsenoside Rg1 in promoting the differentiation of mouse adipose-derived stem cells (mADSC) towards the neuronal lineage. The central nervous system has long been regarded as incapable of self-repair; therefore neuronal differentiation from stem cells is of great interest. However, the use of embryonic stem cells is limited due to their inaccessibility and for ethical reasons, so the search is on for alternative pluripotent cells capable of differentiating into neuronal cells. Adipose-derived stem cells (ADSC) can differentiate into different cell types, including neuronal cells: their accessibility, low risk, and capacity for long-term growth and self-renewal have made them the preferred stem cell type for clinical applications...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471117/antepartum-hemorrhage-from-previous-cesarean-sectioned-uterus-as-a-potential-sign-of-uterine-artery-pseudoaneurysm
#15
Ning Zhang, Wei-Hua Lou, Xue-Bin Zhang, Jian-Hua Lin, Wen Di
Postpartum hemorrhage (PPH), a leading cause of maternal mortality, can occur within 24 h of delivery (primary PPH), or during the period from 24 h after delivery to Week 6 of puerperium (secondary PPH). It requires health professionals to be alert to the symptoms to ensure prompt diagnosis and treatment, especially in the case of rupture of a uterine artery pseudoaneurysm (UAP) due to its life-threatening consequence (Baba et al., 2014). Most of the published case reports or case serials describe UAP as a possible cause of delayed PPH after traumatic procedures during delivery or pregnancy termination, including cesarean section (CS), manual removal of the placenta, or dilation and curettage (D&C) (Wald, 2003)...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471116/no-evidence-of-seed-transmissibility-of-tomato-yellow-leaf-curl-virus-in-nicotiana-benthamiana
#16
Tábata Rosas-Díaz, Dan Zhang, Rosa Lozano-Durán
Recent work reported that Tomato yellow leaf curl virus (TYLCV) is seed-transmissible in tomato, contrary to previous belief. In this work, we explore whether TYLCV is also a seed-borne virus in another member of the Solanaceae family, the experimental host Nicotiana benthamiana.
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471115/clinical-characteristics-and-treatment-of-thyroid-cancer-in-children-and-adolescents-a-retrospective-analysis-of-83-patients
#17
Xiao-Chun Mao, Wen-Qiao Yu, Jin-Biao Shang, Ke-Jing Wang
OBJECTIVE: To study the clinical characteristics, treatment, and prognosis of thyroid cancer in children and adolescents. METHODS: We performed a retrospective analysis of clinical data from 83 cases of thyroid cancer in children and adolescents from January 1990 to December 2010. We compared extra-thyroid extension, lymph node metastasis, distant metastasis, and prognosis between pediatric patients ≤12 years of age (27 cases) and those >12 years of age (56 cases)...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471114/phenotype-genotype-correlation-with-sanger-sequencing-identified-retinol-dehydrogenase-12-rdh12-compound-heterozygous-variants-in-a-chinese-family-with-leber-congenital-amaurosis
#18
Yun Li, Qing Pan, Yang-Shun Gu
BACKGROUND: Leber congenital amaurosis (LCA) is a group of clinically and genetically heterogeneous retinal dystrophy. To date, 22 genes are known to be responsible for LCA, and some specific phenotypic features could provide significant prognostic information for a potential genetic etiology. This study is to identify gene variants responsible for LCA in a Chinese family using direct Sanger sequencing, with the help of phenotype-genotype correlations. METHODS: A Chinese family with six members including two individuals affected with LCA was studied...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471113/antibacterial-mechanism-of-high-mobility-group-nucleosomal-binding-domain-2-on-the-gram-negative-bacteria-escherichia-coli
#19
Heng Li, Xiao-Fei Shen, Xin-E Zhou, Yan-E Shi, Lu-Xia Deng, Yi Ma, Xiao-Ying Wang, Jing-Yu Li, Ning Huang
OBJECTIVE: To investigate the antibacterial mechanism of high-mobility group nucleosomal-binding domain 2 (HMGN2) on Escherichia coli K12, focusing on the antibacterial and antibiofilm formation effects. Its chemotactic activity on human neutrophils was also investigated. METHODS: Human tissue-derived HMGN2 (tHMGN2) was extracted from fresh uterus fiber cystadenoma and purified by HP1100 reversed-phase high-performance liquid chromatography (RP-HPLC). Recombinant human HMGN2 (rHMGN2) was generated in E...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28471112/immune-recovery-after-fluid-resuscitation-in-rats-with-severe-hemorrhagic-shock
#20
Feng Yao, Yuan-Qiang Lu, Jiu-Kun Jiang, Lin-Hui Gu, Han-Zhou Mou
OBJECTIVE: To investigate the effects of resuscitation with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES) on regulatory T cells (Tregs), helper T 1 (Th1)/Th2 and cytotoxic T 1 (Tc1)/Tc2 profiles in the treatment of hemorrhagic shock. METHODS: Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS (n=8), HTS (n=8), or HES (n=8); sham (n=8) and naive control (n=8) groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min...
May 2017: Journal of Zhejiang University. Science. B
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