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Journal of Zhejiang University. Science. B

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https://www.readbyqxmd.com/read/28681589/toxic-epidermal-necrolysis-after-dactinomycin-and-vincristine-combination-chemotherapy-for-nephroblastoma
#1
Yuan Liang, Zhou Yang, Zi-Gang Xu, Lin Ma
In this study, we describe a 2-year-old boy patient with nephroblastoma who has developed toxic epidermal necrolysis (TEN) associated with the combination chemotherapy administration of dactinomycin and vincristine. A skin biopsy confirmed the diagnosis of TEN, and with methylprednisolone pulse therapy, intravenous immunoglobulin (IVIG), and supportive care, the patient improved significantly.
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681588/response-of-linear-and-cyclic-electron-flux-to-moderate-high-temperature-and-high-light-stress-in-tomato
#2
Tao Lu, Jie-Wei Shi, Zhou-Ping Sun, Ming-Fang Qi, Yu-Feng Liu, Tian-Lai Li
OBJECTIVE: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. METHODS: Six-leaf-stage tomato seedlings ("Liaoyuanduoli", n=160) were divided into four parts: Part 1, served as control under 25 °C, 500 µmol/(m(2)·s); Part 2, spayed with distilled water (H2O) under 35 °C, 1000 µmol/(m(2)·s) (HH); Part 3, spayed with 100 µmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 µmol/L methyl viologen (MV, LEF inhibitor) under HH...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681587/functional-characterization-of-the-promoter-of-carbonyl-reductase-1-gene-in-porcine-endometrial-cells
#3
Ai-Ling Zhang, Xian-Yue Sun, Qi Yin, Jian-Hua Zeng, Zhe Zhang, Jia-Qi Li, Hao Zhang
Prostaglandins (PGs) play a critical role in porcine reproduction, of which prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 (CBR1) catalyzes the conversion of PGE2 to PGF2α. A high ratio of PGE2:PGF2α is beneficial to the establishment and maintenance of porcine pregnancy. PG is essential for the establishment of pregnancy which resembles the proinflammatory response and nuclear factor κB (NF-κB) is involved in the process...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681586/effects-on-cytotoxicity-and-antibacterial-properties-of-the-incorporations-of-silver-nanoparticles-into-the-surface-coating-of-dental-alloys
#4
Xiao-Ting Shen, Yan-Zhen Zhang, Fang Xiao, Jing Zhu, Xiao-Dong Zheng
The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO3+NaBH4→ Ag+1/2H2+1/2B2H6+NaNO3. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681585/a-simple-method-for-removing-low-density-granulocytes-to-purify-t-lymphocytes-from-peripheral-blood-mononuclear-cells
#5
Si-Gong Zhang, Yu-Xin Song, Xiao-Ming Shu, Hai-Li Shen, Han-Bo Yang, Rui-Xue Duo, Guo-Chun Wang
OBJECTIVE: Low-density granulocytes (LDGs) can form neutrophil extracellular traps (NETs) spontaneously and excessively. When peripheral blood mononuclear cells (PBMCs) are used for studying T lymphocytes, LDGs contained in the PBMCs may decrease the threshold of activating T lymphocytes by forming NETs. This study focused on the profiles of LDGs in common autoimmune diseases and methods for removing LDGs from PBMCs. METHODS: The percentages of LDGs in PBMCs from 55 patients with dermatomyositis (DM), 15 with polymyositis (PM), 42 with rheumatoid arthritis (RA), 25 with systemic lupus erythematosus (SLE), and 19 healthy controls were determined by flow cytometry...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681584/cytotoxicity-of-anti-tumor-herbal-marsdeniae-tenacissimae-extract-on-erythrocytes
#6
Ke Hao, Bing-Yu Chen, Kai-Qiang Li, Yu Zhang, Cai-Xia Li, Ying Wang, Lu-Xi Jiang, Jiang Shen, Xiang-Chai Guo, Wei Zhang, Meng-Hua Zhu, Zhen Wang
Marsdeniae tenacissimae extract (MTE) has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species (ROS) level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681583/protective-mechanisms-of-hypaconitine-and-glycyrrhetinic-acid-compatibility-in-oxygen-and-glucose-deprivation-injury
#7
Li-Qin Wang, Yu He, Hao-Fang Wan, Hui-Fen Zhou, Jie-Hong Yang, Hai-Tong Wan
This study investigated the protective effect of the compatibility of hypaconitine (HA) and glycyrrhetinic acid (GA) on H9c2 cells under oxygen and glucose deprivation (OGD)-induced injury, and the possible mechanisms. We found that HA+GA significantly improved pathology and morphology of the nucleus and ultrastructure of H9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy (TEM) tests. It also reduced the releases of lactate dehydrogenase (LDH), creatine kinase-myocardial band isoenzyme (CK-MB), and aspartate transaminase (AST) from the cultured supernatant of H9c2 cells, which were tested by enzyme-linked immune sorbent assay (ELISA) kits...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681582/factors-affecting-cerebrospinal-fluid-opening-pressure-in-patients-with-spontaneous-intracranial-hypotension
#8
Ling-Ling Yao, Xing-Yue Hu
OBJECTIVE: Spontaneous intracranial hypotension (SIH) is recognized far more commonly than ever before. Though usually characterized by low cerebrospinal fluid (CSF) pressure, some patients with SIH are observed to have normal pressure values. In this study, we aimed to confirm the proportion of patients with normal CSF opening pressure (CSF OP) and explore the factors affecting CSF OP in SIH patients. METHODS: We retrospectively reviewed 206 consecutive SIH patients and analyzed their clinical and imaging variables (including demographic data, body mass index (BMI), duration of symptoms, and brain imaging findings)...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681581/risk-analysis-of-new-oral-anticoagulants-for-gastrointestinal-bleeding-and-intracranial-hemorrhage-in-atrial-fibrillation-patients-a-systematic-review-and-network-meta-analysis
#9
Wei-Wei Xu, Shen-Jiang Hu, Tao Wu
BACKGROUND: Antithrombotic therapy using new oral anticoagulants (NOACs) in patients with atrial fibrillation (AF) has been generally shown to have a favorable risk-benefit profile. Since there has been dispute about the risks of gastrointestinal bleeding (GIB) and intracranial hemorrhage (ICH), we sought to conduct a systematic review and network meta-analysis using Bayesian inference to analyze the risks of GIB and ICH in AF patients taking NOACs. METHODS: We analyzed data from 20 randomized controlled trials of 91 671 AF patients receiving anticoagulants, antiplatelet drugs, or placebo...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28681580/retrospective-cytological-evaluation-of-indeterminate-thyroid-nodules-according-to-the-british-thyroid-association-2014-classification-and-comparison-of-clinical-evaluation-and-outcomes
#10
Massimo Giusti, Barbara Massa, Margherita Balestra, Paola Calamaro, Stefano Gay, Simone Schiaffino, Giovanni Turtulici, Simonetta Zupo, Eleonora Monti, Gianluca Ansaldo
The cytology of 130 indeterminate nodules (Thy 3) was retrospectively reviewed according to the British Thyroid Association 2014 classification. Nodules were divided into Thy 3a (atypical features) and Thy 3f (follicular lesion) categories. Histology was available as a reference for 97 nodules. Pre-surgical evaluations comprised biochemical tests, color-Doppler ultrasonography (US), semi-quantitative elastography-US (USE), contrast-enhanced US (CEUS), and mutation analysis from cytological slides. Thyroid malignancy was the final diagnosis for 19% of surgically-treated nodules...
July 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585432/full-genome-analysis-of-swine-genotype-3-hepatitis-e-virus-isolated-from-eastern-china
#11
Jiang-Bing Shuai, Lu-Huan Li, Ai-Yun Li, Yong-Qiang He, Xiao-Feng Zhang
Hepatitis E is believed to occur in both endemic and sporadic forms in developing countries, which causes a major public health problem in Asia and Africa (Meng, 2010; Wang et al., 2016a). Recent studies have documented that the disease is also endemic in many industrialized countries (Wenzel et al., 2011). The causative agent, hepatitis E virus (HEV), belonging to the genus Orthohepevirus, is a non-enveloped RNA virus with a single-stranded, positive-sense genome of approximately 7.2 kb (Smith et al., 2014)...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585431/sensory-quality-evaluation-for-appearance-of-needle-shaped-green-tea-based-on-computer-vision-and-nonlinear-tools
#12
Chun-Wang Dong, Hong-Kai Zhu, Jie-Wen Zhao, Yong-Wen Jiang, Hai-Bo Yuan, Quan-Sheng Chen
Tea is one of the three greatest beverages in the world. In China, green tea has the largest consumption, and needle-shaped green tea, such as Maofeng tea and Sparrow Tongue tea, accounts for more than 40% of green tea (Zhu et al., 2017). The appearance of green tea is one of the important indexes during the evaluation of green tea quality. Especially in market transactions, the price of tea is usually determined by its appearance (Zhou et al., 2012). Human sensory evaluation is usually conducted by experts, and is also easily affected by various factors such as light, experience, psychological and visual factors...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585430/satisfactory-clinical-outcome-following-delayed-repositioning-of-a-traumatic-post-lasik-flap-with-dislocation-and-shrinkage-managed-by-irrigation-stretching-and-debridement
#13
Ye-Sheng Xu, Wen-Jia Xie, Yu-Feng Yao
OBJECTIVE: To report surgical management and favorable outcome in a case with delayed repair of traumatic laser in situ keratomileusis (LASIK) flap dislocation with shrinkage and folds. METHODS: A 30-year-old man with a five-year history of bilateral LASIK experienced blunt trauma to his right eye followed by decreased vision for 5 weeks. The surgical management included initially softening the flap by irrigation with balanced salt solution (BSS). The shrinkage folds were carefully and gently stretched by scraping with a 26-gauge cannula accompanied by BSS irrigation...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585429/laparoscopic-versus-open-distal-pancreatectomy-for-pancreatic-ductal-adenocarcinoma-a-single-center-experience
#14
Ai-Bin Zhang, Ye Wang, Chen Hu, Yan Shen, Shu-Sen Zheng
OBJECTIVE: The aim of this study was to compare complications and oncologic outcomes of patients undergoing laparoscopic distal pancreatectomy (LDP) and open distal pancreatectomy (ODP) at a single center. METHODS: Distal pancreatectomies performed for pancreatic ductal adenocarcinoma during a 4-year period were included in this study. A retrospective analysis of a database of this cohort was conducted. RESULTS: Twenty-two patients underwent LDP for pancreatic ductal adenocarcinoma, in comparison to seventy-six patients with comparable tumor characteristics treated by ODP...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585428/anti-hepatocarcinoma-activity-of-tt-1-an-analog-of-melittin-combined-with-interferon-%C3%AE-via-promoting-the-interaction-of-nkg2d-and-mica
#15
Lan-Lan Wan, Da-Qi Zhang, Jin-Nan Zhang, Li-Qun Ren
Hepatocarcinoma is one of the malignant cancers with significant morbidity and mortality. Immunotherapy has emerged in clinical treatment, owing to the limitation and severe side effects of chemotherapy. In the immune system, natural killer (NK) cells are important effectors required to eliminate malignant tumor cells without the limitation of major histocompatibility complex (MHC) molecule issues. Hence, treatment which could stimulate NK cells is of great interest. Here, we investigated the efficacy of the combined therapy of TT-1 (a mutant of melittin) and interferon-α (IFN-α) on NK cells and human liver cancer HepG-2/Huh7 cells in vitro and in vivo, as well as the mechanism involved...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585427/uptake-transport-and-distribution-of-molybdenum-in-two-oilseed-rape-brassica-napus-l-cultivars-under-different-nitrate-ammonium-ratios
#16
Shi-Yu Qin, Xue-Cheng Sun, Cheng-Xiao Hu, Qi-Ling Tan, Xiao-Hu Zhao
OBJECTIVES: To investigate the effects of different nitrate sources on the uptake, transport, and distribution of molybdenum (Mo) between two oilseed rape (Brassica napus L.) cultivars, L0917 and ZS11. METHODS: A hydroponic culture experiment was conducted with four nitrate/ammonium (NO3(-):NH4(+)) ratios (14:1, 9:6, 7.5:7.5, and 1:14) at a constant nitrogen concentration of 15 mmol/L. We examined Mo concentrations in roots, shoots, xylem and phloem sap, and subcellular fractions of leaves to contrast Mo uptake, transport, and subcellular distribution between ZS11 and L0917...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585426/effect-of-helichrysum-plicatum-dc-subsp-plicatum-ethanol-extract-on-gentamicin-induced-nephrotoxicity-in-rats
#17
Betul Apaydin Yildirim, Saban Kordali, Kubra Asena Terim Kapakin, Fatih Yildirim, Esra Aktas Senocak, Serdar Altun
The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585425/cide-gene-expression-in-adipose-tissue-liver-and-skeletal-muscle-from-obese-and-lean-pigs
#18
Yue-Qin Qiu, Xue-Fen Yang, Xian-Yong Ma, Yun-Xia Xiong, Zhi-Mei Tian, Qiu-Li Fan, Li Wang, Zong-Yong Jiang
The expression of the cell death-inducing DNA fragmentation factor α-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes' expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585424/punicalagin-protects-bovine-endometrial-epithelial-cells-against-lipopolysaccharide-induced-inflammatory-injury
#19
An Lyu, Jia-Jia Chen, Hui-Chuan Wang, Xiao-Hong Yu, Zhi-Cong Zhang, Ping Gong, Lin-Shu Jiang, Feng-Hua Liu
OBJECTIVE: Bovine endometritis is one of the most common reproductive disorders in cattle. The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and to uncover the underlying mechanisms. METHODS: bEECs were stimulated with different concentrations (1, 10, 30, 50, and 100 μg/ml) of LPS for 3, 6, 9, 12, and 18 h. MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28585423/copper-modified-palygorskite-is-effective-in-preventing-and-treating-diarrhea-caused-by-salmonella-typhimurium
#20
Da-Wei Yao, Ze-Zhong Yu, Na Li, Yu-Nong Hou, Jia-Rong Xu, De-Ji Yang
The aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. In this study, the efficacy of copper-modified palygorskite (CM-Pal) in preventing diarrhea caused by Salmonella was specifically examined both in vitro and in vivo. The CM-Pal was prepared with palygorskite (Pal) and copper nitrate. The antibacterial activity of the CM-Pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the CM-Pal to Salmonella typhimurium cultures...
June 2017: Journal of Zhejiang University. Science. B
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