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Cytometry. Part A: the Journal of the International Society for Analytical Cytology

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https://www.readbyqxmd.com/read/29451717/modeling-of-cytometry-data-in-logarithmic-space-when-is-a-bimodal-distribution-not-bimodal
#1
Amir Erez, Robert Vogel, Andrew Mugler, Andrew Belmonte, Grégoire Altan-Bonnet
Recent efforts in systems immunology lead researchers to build quantitative models of cell activation and differentiation. One goal is to account for the distributions of proteins from single-cell measurements by flow cytometry or mass cytometry as readout of biological regulation. In that context, large cell-to-cell variability is often observed in biological quantities. We show here that these readouts, viewed in logarithmic scale may result in two easily-distinguishable modes, while the underlying distribution (in linear scale) is unimodal...
February 16, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29409121/novel-aspects-of-live-intestinal-epithelial-cell-function-revealed-using-a-custom-time-lapse-video-microscopy-apparatus
#2
Michael Papetti, Piotr Kozlowski
Many aspects of cell physiology, including migration, membrane function, and cell division, are best understood by observing live cell dynamics over time using video microscopy. To probe these phenomena in colon epithelial cells using simple components with a limited budget, we have constructed an inexpensive (<$410) self-contained apparatus, consisting of a closed-loop, feedback-controlled system regulated by a PID (proportional-integrative-derivative) controller contained within a 0.077 m3 insulated acrylic box...
February 6, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29389074/turning-noise-into-order-on-the-cell-surface-resonant-activation-of-molecular-highlighters
#3
György Vámosi, László Damjanovich, László Bene
No abstract text is available yet for this article.
February 1, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29381264/passage-culture-of-human-monocyte-macrophage-lineage-cells-using-a-temperature-responsive-culture-dish
#4
LETTER
Hirotaka Suga, Erina Kurita, Isao Kurachi, Akihiko Takushima
No abstract text is available yet for this article.
January 30, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29364561/analysis-of-ntpdase2-in-the-cell-membrane-using-fluorescence-recovery-after-photobleaching-frap
#5
Franciele Cristina Kipper, Alessandra Sayuri Kikuchi Tamajusuku, Darlan Conterno Minussi, José Eduardo Vargas, Ana Maria Oliveira Battastini, Elzbieta Kaczmarek, Simon Christopher Robson, Guido Lenz, Márcia Rosângela Wink
NTPDase2, a member of the CD39/NTPDase family, is an ecto-nucleotidase anchored to the plasma membrane by two transmembrane domains, with a catalytic site facing the extracellular space and preferentially hydrolyzing nucleoside triphosphates. While NTPDase2 is expressed in many cell types, its unique functionality, mobility and dynamics at the cell membrane remain unexplored. We therefore constructed a recombinant NTPDase2 linked to the yellow fluorescent protein (EYFP) to investigate its dynamics by confocal microscopy...
January 24, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29356334/omip-044-28-color-immunophenotyping-of-the-human-dendritic-cell-compartment
#6
Florian Mair, Martin Prlic
No abstract text is available yet for this article.
January 22, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29356320/deconvolution-models-for-a-better-understanding-of-natural-microbial-communities-enumerated-by-flow-cytometry
#7
Gianluca Corno, Cristiana Callieri
No abstract text is available yet for this article.
January 22, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29346713/spark-generated-microbubble-cell-sorter-for-microfluidic-flow-cytometry
#8
Jingjing Zhao, Zheng You
High-speed and accurate cell sorting is of great significance for cell analysis regarding both bioresearch and clinical application. Different from the jet-in-air sorting of commercial flow cytometers, sorting in fully enclosed and disposal microfluidic chips can avoid aerosols and crosscontamination, thus contributing to the improvement of biosafety and test accuracy. However, current microfluidic sorters usually require complicated structures, or otherwise cannot attain high throughput. In this article, a sorting mechanism for microfluidics is proposed for the first time based on the jet flow induced by the spark-generated cavitation microbubble that can be easily realized by a pair of electrodes...
January 18, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29345745/comparison-of-flow-cytometric-methods-for-the-enumeration-of-residual-leucocytes-in-leucoreduced-blood-products-a-multicenter-study
#9
Yang Zeng, Michelle Dabay, Virginia George, Shalini Seetharaman, Monika de Arruda Indig, Sharon Graminske, Nicole Kimpel, Anna Schmidt, Amanda Boerner, Sarai Paradiso, Tatyana Delman, Yunyao Li, Viktoriya Litvak, Farzad Oreizy, Angela Chen, Maryam Saleminik, Fred Mosqueda, Anna Lin, Kevin Judge
The BD FACSVia™ System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount™ kit with the BD FACSCalibur™ flow cytometer. Leucoreduced platelet (PLT, n = 252) and red blood cell (RBC, n = 278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter-site reproducibility on the BD FACSVia System at three sites over 20 days...
January 18, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29288606/omip-042-21-color-flow-cytometry-to-comprehensively-immunophenotype-major-lymphocyte-and-myeloid-subsets-in-human-peripheral-blood
#10
Karl W Staser, William Eades, Jaebok Choi, Darja Karpova, John F DiPersio
No abstract text is available yet for this article.
December 30, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29286577/omip-043-identification-of-human-antibody-secreting-cell-subsets
#11
Jeffrey Carrell, Christopher J Groves
No abstract text is available yet for this article.
December 29, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29286574/internal-rulers-to-assess-fluorescent-protein-photoactivation-efficiency
#12
Malte Renz, Christian Wunder
Photoactivatable fluorescent proteins (PA-FPs) have been widely used to assess the dynamics of cell biological processes. In addition, PA-FPs enabled single-molecule based super-resolution imaging (photoactivated localization microscopy) and thereby provided unprecedented structural insight. For the lack of tools, however, the fraction of PA-FPs that is, actually being switched on to fluoresce, that is, the photoactivation efficiency, has been difficult to assess. Uncertainty about photoactivation efficiency has hampered an understanding of the absolute amount of PA-FPs, that is, being examined...
December 29, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29283496/quantitative-assessment-of-cancer-cell-morphology-and-motility-using-telecentric-digital-holographic-microscopy-and-machine-learning
#13
Van K Lam, Thanh C Nguyen, Byung M Chung, George Nehmetallah, Christopher B Raub
The noninvasive, fast acquisition of quantitative phase maps using digital holographic microscopy (DHM) allows tracking of rapid cellular motility on transparent substrates. On two-dimensional surfaces in vitro, MDA-MB-231 cancer cells assume several morphologies related to the mode of migration and substrate stiffness, relevant to mechanisms of cancer invasiveness in vivo. The quantitative phase information from DHM may accurately classify adhesive cancer cell subpopulations with clinical relevance. To test this, cells from the invasive breast cancer MDA-MB-231 cell line were cultured on glass, tissue-culture treated polystyrene, and collagen hydrogels, and imaged with DHM followed by epifluorescence microscopy after staining F-actin and nuclei...
December 28, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29266796/flow-cytometric-fingerprinting-for-microbial-strain-discrimination-and-physiological-characterization
#14
Benjamin Buysschaert, Frederiek-Maarten Kerckhof, Peter Vandamme, Bernard De Baets, Nico Boon
The analysis of microbial populations is fundamental, not only for developing a deeper understanding of microbial communities but also for their engineering in biotechnological applications. Many methods have been developed to study their characteristics and over the last few decades, molecular analysis tools, such as DNA sequencing, have been used with considerable success to identify the composition of microbial populations. Recently, flow cytometric fingerprinting is emerging as a promising and powerful method to analyze bacterial populations...
December 20, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29265528/deconvolution-model-to-resolve-cytometric-microbial-community-patterns-in-flowing-waters
#15
Stefano Amalfitano, Stefano Fazi, Elisabet Ejarque, Anna Freixa, Anna M Romaní, Andrea Butturini
Flow cytometry is suitable to discriminate and quantify aquatic microbial cells within a spectrum of fluorescence and light scatter signals. Using fixed gating and operational settings, we developed a finite distribution mixture model, followed by the Voronoi tessellation, to resolve bivariate cytometric profiles into cohesive subgroups of events. This procedure was applied to outline recurrent patterns and quantitative changes of the aquatic microbial community along a river hydrologic continuum. We found five major subgroups within each of the commonly retrieved populations of cells with Low and High content of Nucleic Acids (namely, LNA, and HNA cells)...
December 19, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29251823/yellow-green-laser-based-flow-cytometry-for-cd34-progenitor-cell-counting
#16
LETTER
Laura G Rico, Jordi Juncà, Mike D Ward, Jolene Bradford, Jordi Petriz
No abstract text is available yet for this article.
December 18, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29236351/adipocytes-role-in-the-bone-marrow-niche
#17
LETTER
Daniel A P Guerra, Ana E Paiva, Isadora F G Sena, Patrick O Azevedo, Miguel Luiz Batista, Akiva Mintz, Alexander Birbrair
Adipocyte infiltration in the bone marrow follows chemotherapy or irradiation. Previous studies indicate that bone marrow fat cells inhibit hematopoietic stem cell function. Recently, Zhou et al. (2017) using state-of-the-art techniques, including sophisticated Cre/loxP technologies, confocal microscopy, in vivo lineage-tracing, flow cytometry, and bone marrow transplantation, reveal that adipocytes promote hematopoietic recovery after irradiation. This study challenges the current view of adipocytes as negative regulators of the hematopoietic stem cells niche, and reopens the discussion about adipocytes' roles in the bone marrow...
December 13, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29220555/multiparameter-cytometric-analysis-of-complex-cellular-response
#18
Šárka Šimečková, Radek Fedr, Ján Remšík, Zuzana Kahounová, Eva Slabáková, Karel Souček
Complex analysis of cellular responses after experimental treatment is important for screening, mechanistic understanding of treatment effects, and the identification of sensitive and resistant cell phenotypes. Modern multicolor flow cytometry has demonstrated its power for such analyses. Here, we introduce a multiparametric protocol for complex analysis of cytokinetics by the simultaneous detection of seven fluorescence parameters. This analysis includes the detection of two surface markers for immunophenotyping, analysis of proliferation based on the cell cycle and the measurement of incorporated nucleoside analogue 5-ethynyl-2'-deoxyuridine (EdU) in newly synthesized DNA, analysis of DNA damage using an anti-phospho-histone H2A...
December 8, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29205767/atomic-mass-tag-of-bismuth-209-for-increasing-the-immunoassay-multiplexing-capacity-of-mass-cytometry
#19
Guojun Han, Shih-Yu Chen, Veronica D Gonzalez, Eli R Zunder, Wendy J Fantl, Garry P Nolan
Mass cytometry (or CyTOF) is an atomic mass spectrometry-based single-cell immunoassay technology, which has provided an increasingly systematic and sophisticated view in basic biological and clinical studies. Using elemental reporters composed of stable heavy metal isotopes, more than 50 cellular parameters are measured simultaneously. However, this current multiplexing does not meet the theoretical capability of CyTOF instrumentation with 135 detectable channels, primarily due to the limitation of available chemistries for conjugating elemental mass tags to affinity reagents...
December 4, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29194963/simultaneous-detection-of-protein-and-mrna-in-jurkat-and-kg-1a-cells-by-mass-cytometry
#20
Anastasia Mavropoulos, Bedilu Allo, Mingxiao He, Emily Park, Daniel Majonis, Olga Ornatsky
Mass cytometry uniquely enables high-dimensional single-cell analysis of complex populations. This recently developed technology is based on inductively coupled time-of-flight mass spectrometry for multiplex proteomic analysis of more than 40 markers per cell. The ability to characterize the transcriptome is critical for the understanding of disease pathophysiology, medical diagnostics, and drug discovery. Current techniques allowing the in situ detection of transcripts in single cells are limited to a small number of simultaneous targets and are generally tedious and labor-intensive...
November 30, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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