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Cytometry. Part A: the Journal of the International Society for Analytical Cytology

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https://www.readbyqxmd.com/read/28323396/toll-like-receptor-2-bright-cells-identify-circulating-monocytes-in-human-and-non-human-primates
#1
Erin N Shirk, Brian G Kral, Lucio Gama
Polychromatic flow cytometry is a useful tool for monitoring circulating whole blood monocytes, although gating strategies often vary depending on the study. Increased analyses of the myeloid system have revealed monocytes to be more plastic than previously understood and uncovered changes among surface markers previously considered to be stable. The myeloid system has also been found to have disparate surface markers between mouse, human, and non-human primate studies, which further complicates examination between species...
March 21, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28323382/need-for-winning-combinations-of-modalities-in-cytometry-of-stem-cells
#2
Zsolt Bacsó
No abstract text is available yet for this article.
March 21, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28314083/characterization-of-dormant-and-active-human-cancer-cells-by-quantitative-phase-imaging
#3
Peng Guo, Jing Huang, Marsha A Moses
The switch of tumor cells from a dormant, non-angiogenic phenotype to an active, angiogenic phenotype is a critical step in early cancer progression. To date, relatively little is known about the cellular behaviors of angiogenic and non-angiogenic tumor cell phenotypes. In this study, holographic imaging cytometry, a quantitative phase imaging (QPI) technique was used to continuously and non-invasively analyze, quantify, and compare a panel of fundamental cellular behaviors of angiogenic and non-angiogenic human osteosarcoma cells (KHOS) in a simple and economical way...
March 17, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28296044/biolens-behavior-of-rbcs-under-optically-induced-mechanical-stress
#4
Francesco Merola, Álvaro Barroso, Lisa Miccio, Pasquale Memmolo, Martina Mugnano, Pietro Ferraro, Cornelia Denz
In this work, the optical behavior of Red Blood Cells (RBCs) under an optically-induced mechanical stress was studied. Exploiting the new findings concerning the optical lens-like behavior of RBCs, the variations of the wavefront refracted by optically-deformed RBCs were further investigated. Experimental analysis have been performed through the combination of digital holography and numerical analysis based on Zernike polynomials, while the biological lens is deformed under the action of multiple dynamic optical tweezers...
March 13, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28295966/three-dimensional-intracellular-transport-in-neuron-bodies-and-neurites-investigated-by-label-free-dispersion-relation-phase-spectroscopy
#5
Mikhail E Kandel, Daniel Fernandes, Alison M Taylor, Haadi Shakir, Catherine Best-Popescu, Gabriel Popescu
Due to the limitations of fluorescence imaging techniques, the study of intracellular cargo is typically restricted to two-dimensional analyses. To overcome low light levels and the risk of phototoxicity, we employ quantitative phase imaging, a family of full-field imaging techniques that measure the optical path length shift introduced by the specimen. Specifically, we use spatial light interference microscopy (SLIM) to study the transport of mass in whole tomographic volumes and show that a time-correlation technique, dispersion-relation phase spectroscopy (DPS), can be used to simultaneously assay the horizontal and vertical traffic of mass through a cell...
March 13, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28264143/a-flowcytometric-analysis-to-efficiently-quantify-multiple-innate-immune-cells-and-t-cell-subsets-in-human-blood
#6
D F Draxler, M T Madondo, G Hanafi, M Plebanski, R L Medcalf
The balance of inflammation and immunosuppression driven by changed ratios in diverse myeloid and T cell subsets, as well as their state of activation and ability to migrate to lymphoid compartments or inflammatory sites, has emerged as a highly active area of study across clinical trials of vaccines and therapies against cancer, trauma, as well as autoimmune and infectious diseases. There is a need for effective protocols which maximally use the possibilities offered by modern flow cytometers to characterize such immune cell changes in peripheral blood using small volumes of human blood...
March 6, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28264140/quantitative-phase-imaging-for-cell-culture-quality-control
#7
Lena Kastl, Michael Isbach, Dieter Dirksen, Jürgen Schnekenburger, Björn Kemper
The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium...
March 6, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28245335/a-method-for-characterizing-phenotypic-changes-in-highly-variable-cell-populations-and-its-application-to-high-content-screening-of-arabidopsis-thaliana-protoplasts
#8
Gregory R Johnson, Joshua D Kangas, Alexander Dovzhenko, Rüdiger Trojok, Karsten Voigt, Timothy D Majarian, Klaus Palme, Robert F Murphy
Quantitative image analysis procedures are necessary for the automated discovery of effects of drug treatment in large collections of fluorescent micrographs. When compared to their mammalian counterparts, the effects of drug conditions on protein localization in plant species are poorly understood and underexplored. To investigate this relationship, we generated a large collection of images of single plant cells after various drug treatments. For this, protoplasts were isolated from six transgenic lines of A...
February 28, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28240818/imaging-of-dense-cell-cultures-by-multiwavelength-lens-free-video-microscopy
#9
C Allier, S Morel, R Vincent, L Ghenim, F Navarro, M Menneteau, T Bordy, L Hervé, O Cioni, X Gidrol, Y Usson, J-M Dinten
They present results for lens-free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm(2) ) over a large field of view of 29.4 mm(2) . Combined with the compactness of the system which fits altogether inside an incubator, lens-free microscopy becomes a unique tool to monitor cell cultures over several days...
February 27, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28240810/ultraviolet-320-nm-laser-excitation-for-flow-cytometry
#10
William Telford, Lynn Stickland, Marco Koschorreck
Although multiple lasers and high-dimensional analysis capability are now standard on advanced flow cytometers, ultraviolet (UV) lasers (usually 325-365 nm) remain an uncommon excitation source for cytometry. This is primarily due to their cost, and the small number of applications that require this wavelength. The development of the Brilliant Ultraviolet (BUV fluorochromes, however, has increased the importance of this formerly niche excitation wavelength. Historically, UV excitation was usually provided by water-cooled argon- and krypton-ion lasers...
February 27, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28192639/a-deep-learning-based-strategy-for-identifying-and-associating-mitotic-activity-with-gene-expression-derived-risk-categories-in-estrogen-receptor-positive-breast-cancers
#11
David Romo-Bucheli, Andrew Janowczyk, Hannah Gilmore, Eduardo Romero, Anant Madabhushi
The treatment and management of early stage estrogen receptor positive (ER+) breast cancer is hindered by the difficulty in identifying patients who require adjuvant chemotherapy in contrast to those that will respond to hormonal therapy. To distinguish between the more and less aggressive breast tumors, which is a fundamental criterion for the selection of an appropriate treatment plan, Oncotype DX (ODX) and other gene expression tests are typically employed. While informative, these gene expression tests are expensive, tissue destructive, and require specialized facilities...
February 13, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28141908/quantitative-tumor-heterogeneity-assessment-on-a-nuclear-population-basis
#12
Anne-Sofie Wessel Lindberg, Knut Conradsen, Rasmus Larsen, Michael Friis Lippert, Rasmus Røge, Mogens Vyberg
Immunohistochemistry Ki-67 stain is widely used for visualizing cell proliferation. The common method for scoring the proliferation is to manually select and score a hot spot. This method is time-consuming and will often not give reproducible results due to subjective selection of the hotspots and subjective scoring. An automatic hotspot detection and proliferative index scoring would be time-saving, make the determination of the Ki-67 score easier and minimize the uncertainty of the score by introducing a more objective and standardized score...
January 31, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28110507/computer-assisted-quantification-of-cd3-t-cells-in-follicular-lymphoma
#13
Fazly S Abas, Arwa Shana'ah, Beth Christian, Robert Hasserjian, Abner Louissaint, Michael Pennell, Berkman Sahiner, Weijie Chen, Muhammad Khalid Khan Niazi, Gerard Lozanski, Metin Gurcan
The advance of high resolution digital scans of pathology slides allowed development of computer based image analysis algorithms that may help pathologists in IHC stains quantification. While very promising, these methods require further refinement before they are implemented in routine clinical setting. Particularly critical is to evaluate algorithm performance in a setting similar to current clinical practice. In this article, we present a pilot study that evaluates the use of a computerized cell quantification method in the clinical estimation of CD3 positive (CD3+) T cells in follicular lymphoma (FL)...
January 22, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28110496/heterogenic-response-of-prokaryotes-toward-silver-nanoparticles-and-ions-is-facilitated-by-phenotypes-and-attachment-of-silver-aggregates-to-cell-surfaces
#14
Yuting Guo, Hans J Stärk, Gerd Hause, Matthias Schmidt, Hauke Harms, Lukas Y Wick, Susann Müller
Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC4 (3) as markers for cell membrane disintegration and depolarization, respectively...
January 22, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28081295/connected-component-masking-accurately-identifies-the-ratio-of-phagocytosed-and-cell-bound-particles-in-individual-cells-by-imaging-flow-cytometry
#15
Chenjie Fei, Dustin M E Lillico, Brian Hall, Aja M Rieger, James L Stafford
Innate immune cell-mediated recognition, capture, and engulfment of large particulate targets such as bacteria is known as phagocytosis. This highly dynamic cellular process involves a series of steps including receptor-mediated target binding, phagocytic cup formation, pseudopod extension, and phagosome closure, which depend on distinct actin polymerization events. Using flow cytometry, precise determination of target locations relative to cell membranes (i.e., surface-bound vs. fully engulfed/internalized) during the phagocytic process is difficult to quantify...
January 12, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28324631/measuring-the-vicious-ones
#16
EDITORIAL
Attila Tárnok
No abstract text is available yet for this article.
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28281330/basophil-activation-test-implementation-and-standardization-between-systems-and-between-instruments
#17
Anne-Emmanuelle Depince-Berger, Khaled Sidi-Yahya, Mohammed Jeraiby, Claude Lambert
The basophil activation test (BAT) is a good ex vivo alternative for measuring hypersensitivity to an allergen in sensitized patients but still lacks standardization. In this present study, we have implemented one of the systems and proposed inter-systems, inter-instrument standardization. Our method for basophil activation and labeling on whole blood: EDTA in one step using BasoflowEx(®) and FlowCast(®) . Setup on Navios and fluorescence targets converted to set up FACSCanto™ instrument. Our results: 1) A CD203c/CD63 (BasoflowEx) method was adapted for EDTA samples and simplified...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28248454/combination-of-imaging-flow-cytometry-and-time-lapse-microscopy-for-the-study-of-label-free-morphology-dynamics-of-hematopoietic-cells
#18
Jérémie Cosette, Alice Moussy, Andras Paldi, Daniel Stockholm
Cell differentiation is a longitudinal and dynamic process. Studying and quantifying such a process require tools combining precise time resolution and statistical power. Imaging flow cytometry (IFC) provides statistically significant number of microscopy images of individual cells in a sample at a given time point. Time-lapse microscopy (TLM) is the method of choice for studying the dynamics of cell processes at a high temporal, but low statistical resolution. In this work, we show that the dynamic changes of cord-blood derived CD34+ cells in response to cytokine stimulation can be successfully studied, in a label-free way, by the combination of the IFCs statistical power and the TLM's high time resolution...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28234411/toward-deterministic-and-semiautomated-spade-analysis
#19
Peng Qiu
SPADE stands for spanning-tree progression analysis for density-normalized events. It combines downsampling, clustering and a minimum-spanning tree to provide an intuitive visualization of high-dimensional single-cell data, which assists with the interpretation of the cellular heterogeneity underlying the data. SPADE has been widely used for analysis of high-content flow cytometry data and CyTOF data. The downsampling and clustering components of SPADE are both stochastic, which lead to stochasticity in the tree visualization it generates...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28207983/a-novel-flow-cytometric-application-discriminates-among-the-effects-of-chemical-inhibitors-on-various-phases-of-babesia-divergens-intraerythrocytic-cycle
#20
Jeny R Cursino-Santos, Manpreet Singh, Petra Pham, Cheryl A Lobo
Human babesiosis is a global emerging infectious disease caused by intraerythrocytic parasites of the genus Babesia. Its biology has remained largely unexplored due to a lack of critical tools and techniques required to define the various stages and phases of the parasite's cycle in its host RBC and the interplay between host and parasite. This article presents a powerful set of tools combining stage synchronization of the parasite with a platform that encompasses both a flow cytometric evaluation of the subpopulation structure of the parasite population together with a morphological assessment of the population parasites using light microscopy of conventional Giemsa stained smears...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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