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Cytometry. Part A: the Journal of the International Society for Analytical Cytology

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https://www.readbyqxmd.com/read/28081295/connected-component-masking-accurately-identifies-the-ratio-of-phagocytosed-and-cell-bound-particles-in-individual-cells-by-imaging-flow-cytometry
#1
Chenjie Fei, Dustin M E Lillico, Brian Hall, Aja M Rieger, James L Stafford
Innate immune cell-mediated recognition, capture, and engulfment of large particulate targets such as bacteria is known as phagocytosis. This highly dynamic cellular process involves a series of steps including receptor-mediated target binding, phagocytic cup formation, pseudopod extension, and phagosome closure, which depend on distinct actin polymerization events. Using flow cytometry, precise determination of target locations relative to cell membranes (i.e., surface-bound vs. fully engulfed/internalized) during the phagocytic process is difficult to quantify...
January 12, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28075531/three-dimensional-imaging-flow-cytometry-through-light-sheet-fluorescence-microscopy
#2
REVIEW
Emilio J Gualda, Hugo Pereira, Gabriel G Martins, Rui Gardner, Nuno Moreno
Flow cytometry is the tool of choice for high-speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two-dimensional spatial information were developed, some of the limitations remained though, namely constrains related to sample size and lack of depth or dynamic information. The combination of fluidics and light-sheet illumination has the potential to address these limitations...
January 11, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28009470/proportion-of-circulating-tumor-cell-clusters-increases-during-cancer-metastasis
#3
Yuanzhen Suo, Chengying Xie, Xi Zhu, Zhichao Fan, Zhangru Yang, Hao He, Xunbin Wei
Circulating tumor cell (CTC) clusters are found among CTCs and show significantly greater potential for an important role in cancer metastasis than single CTCs, which have been traditionally believed as the majority of CTCs. The accurate proportion and dynamics of CTC clusters remain unclear due to the fact that CTCs in blood flow are very difficult to detect in vivo and in vitro. CTC clusters are even more difficult to be distinguished from CTCs without perturbation by state-of-the-art detection methods. Here, we demonstrate that by using in vivo flow cytometry (IVFC), we can reliably measure the proportion and dynamics of CTC clusters in two murine tumor models...
December 23, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28009468/simultaneous-automatic-scoring-and-co-registration-of-hormone-receptors-in-tumor-areas-in-whole-slide-images-of-breast-cancer-tissue-slides
#4
Nicholas Trahearn, Yee Wah Tsang, Ian A Cree, David Snead, David Epstein, Nasir Rajpoot
Automation of downstream analysis may offer many potential benefits to routine histopathology. One area of interest for automation is in the scoring of multiple immunohistochemical markers to predict the patient's response to targeted therapies. Automated serial slide analysis of this kind requires robust registration to identify common tissue regions across sections. We present an automated method for co-localized scoring of Estrogen Receptor and Progesterone Receptor (ER/PR) in breast cancer core biopsies using whole slide images...
December 23, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27984679/making-the-cut-innovative-methods-for-optimizing-perfusion-based-migration-assays
#5
Andrew W Holt, William E Howard, Elizabeth T Ables, Stephanie M George, Cindy A Kukoly, Jake E Rabidou, Jake T Francisco, Angel N Chukwu, David A Tulis
Application of fluid shear stress to adherent cells dramatically influences their cytoskeletal makeup and differentially regulates their migratory phenotype. Because cytoskeletal rearrangements are necessary for cell motility and migration, preserving these adaptations under in vitro conditions and in the presence of fluid flow are physiologically essential. With this in mind, parallel plate flow chambers and microchannels are often used to conduct in vitro perfusion experiments. However, both of these systems currently lack capacity to accurately study cell migration in the same location where cells were perfused...
December 16, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27911980/cell-based-dna-demethylation-detection-system-for-screening-of-epigenetic-drugs-in-2d-3d-and-xenograft-models
#6
Khushboo Agrawal, Viswanath Das, Miroslav Otmar, Marcela Krečmerová, Petr Džubák, Marián Hajdúch
Aberrant DNA methylation that results in silencing of genes has remained a significant interest in cancer research. Despite major advances, the success of epigenetic therapy is elusive due to narrow therapeutic window. A wide variety of naturally occurring epigenetic agents and synthetic molecules that can alter methylation patterns exist, however, their usefulness in epigenetic therapy remains unknown. This underlines the need for effective tumor models for large-scale screening of drug candidates with potent hypomethylation activity...
December 2, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27911977/selective-inactivation-of-enzymes-conjugated-to-nanoparticles-using-tuned-laser-illumination
#7
Asaf Ilovitsh, Pazit Polak, Zeev Zalevsky, Orit Shefi
We report a novel method for specific deactivation of conjugated enzymes using laser-heated gold nanoparticles. Current methods involve treatment of the entire solution, thereby inactivating all bioactive components. Our method enables inactivation of only a single or subset of targeted enzymes. The selected enzyme is pre-conjugated to gold nanoparticles, which are specifically heated by a laser tuned to their surface plasmon resonance. We demonstrate inactivation of a selected enzyme, glucose oxidase, within a mixture of biomolecules...
December 2, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27798817/optimization-of-mass-cytometry-sample-cryopreservation-after-staining
#8
Hermi R Sumatoh, Karen Wei Weng Teng, Yang Cheng, Evan W Newell
The advent of mass cytometry has facilitated highly multi-parametric single-cell analysis allowing for the deep assessment of cellular diversity. While the data and analytical power of this approach are well described, associated technical and experimental hurdles remain. Issues like equipment breakdown and sampling of large-scale batches, which may require multiple days of data acquisition, are minor but critical obstacles that prompt a technical solution, especially when dealing with precious samples. An ability to cryopreserve mass cytometry samples that have already been stained would alleviate numerous technical limitations we face with currently used sample-handling approaches...
October 31, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27768827/cell-size-assays-for-mass-cytometry
#9
Alan D Stern, Adeeb H Rahman, Marc R Birtwistle
Mass cytometry offers the advantage of allowing the simultaneous measurement of a greater number parameters than conventional flow cytometry. However, to date, mass cytometry has lacked a reliable alternative to the light scatter properties that are commonly used as a cell size metric in flow cytometry (forward scatter intensity-FSC). Here, we report the development of two plasma membrane staining assays to evaluate mammalian cell size in mass cytometry experiments. One is based on wheat germ agglutinin (WGA) staining and the other on Osmium tetroxide (OsO4 ) staining, both of which have preferential affinity for cell membranes...
October 21, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28002657/sometimes-simpler-is-better-vlog-a-general-but-easy-to-implement-log-like-transform-for-cytometry
#10
C Bruce Bagwell, Beth L Hill, Donald J Herbert, Chris M Bray, Benjamin C Hunsberger
The fundamental purpose of log and log-like transforms for cytometry is to make measured population variabilities as uniform as possible. The long-standing success of the log transform was its ability to stabilize linearly increasing gain-dependent uncertainties and the success of the log-like transforms is that they extend this notion to include zero and negative measurement values. This study derives and examines a transform called VLog that stabilizes the three general sources of variability: (1) gain-dependent variability, (2) photo-electron counting error, and (3) signal-independent sources of error...
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28002656/cytometry-advancement-a-perspective-from-china
#11
EDITORIAL
Xuantao Su, Attila Tárnok
No abstract text is available yet for this article.
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27992111/comparison-of-clustering-methods-for-high-dimensional-single-cell-flow-and-mass-cytometry-data
#12
Lukas M Weber, Mark D Robinson
Recent technological developments in high-dimensional flow cytometry and mass cytometry (CyTOF) have made it possible to detect expression levels of dozens of protein markers in thousands of cells per second, allowing cell populations to be characterized in unprecedented detail. Traditional data analysis by "manual gating" can be inefficient and unreliable in these high-dimensional settings, which has led to the development of a large number of automated analysis methods. Methods designed for unsupervised analysis use specialized clustering algorithms to detect and define cell populations for further downstream analysis...
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27922735/virtual-cell-imaging-a-review-on-simulation-methods-employed-in-image-cytometry
#13
REVIEW
Vladimír Ulman, David Svoboda, Matti Nykter, Michal Kozubek, Pekka Ruusuvuori
The simulations of cells and microscope images thereof have been used to facilitate the development, selection, and validation of image analysis algorithms employed in cytometry as well as for modeling and understanding cell structure and dynamics beyond what is visible in the eyepiece. The simulation approaches vary from simple parametric models of specific cell components-especially shapes of cells and cell nuclei-to learning-based synthesis and multi-stage simulation models for complex scenes that simultaneously visualize multiple object types and incorporate various properties of the imaged objects and laws of image formation...
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27875652/sample-preparation-for-flow-cytometry-benefits-from-some-lateral-thinking
#14
Andrew Filby
No abstract text is available yet for this article.
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27875619/automated-leukocyte-processing-by-microfluidic-deterministic-lateral-displacement
#15
Curt I Civin, Tony Ward, Alison M Skelley, Khushroo Gandhi, Zendra Peilun Lee, Christopher R Dosier, Joseph L D'Silva, Yu Chen, MinJung Kim, James Moynihan, Xiaochun Chen, Lee Aurich, Sergei Gulnik, George C Brittain, Diether J Recktenwald, Robert H Austin, James C Sturm
We previously developed a Deterministic Lateral Displacement (DLD) microfluidic method in silicon to separate cells of various sizes from blood (Davis et al., Proc Natl Acad Sci 2006;103:14779-14784; Huang et al., Science 2004;304:987-990). Here, we present the reduction-to-practice of this technology with a commercially produced, high precision plastic microfluidic chip-based device designed for automated preparation of human leukocytes (white blood cells; WBCs) for flow cytometry, without centrifugation or manual handling of samples...
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27632791/when-polychromatic-flow-cytometry-meets-mitochondrial-reactive-oxygen-species
#16
Katarzyna Piwocka
No abstract text is available yet for this article.
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27575554/quantification-of-mitochondrial-reactive-oxygen-species-in-living-cells-by-using-multi-laser-polychromatic-flow-cytometry
#17
Sara De Biasi, Lara Gibellini, Elena Bianchini, Milena Nasi, Marcello Pinti, Stefano Salvioli, Andrea Cossarizza
Reactive oxygen species (ROS) are constantly produced in cells, mainly by mitochondria, as a consequence of aerobic respiration. Most ROS derive from superoxide, which is rapidly converted to hydrogen peroxide. ROS are involved in the regulation of several physiological and pathological processes, and the possibility to measure them simultaneously is needed, when the redox status of the cells is modified by experimental/biological conditions. Flow cytometry is the main technology that generates multiple information at the single cell level in a high-throughput manner, and gives rapid and quantitative measurements of different ROS with high sensitivity and reproducibility...
December 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27870536/teaching-advanced-flow-cytometry-in-africa-10-years-of-lessons-learned
#18
Elisa Nemes, Wendy A Burgers, Catherine Riou, Erica Andersen-Nissen, Guido Ferrari, Clive M Gray, Thomas Scriba
No abstract text is available yet for this article.
November 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27870535/re-visiting-fc-receptor-blocking-maneuvers-in-man
#19
Paul J Smith, Pratip K Chattopadhyay
No abstract text is available yet for this article.
November 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/27870534/in-memoriam-bernard-bernie-a-shoor
#20
Donna Arndt-Jovin, Tom Jovin, L Scott Cram, Joe Gray, James Jett, Brian Mayall, Ben Verwer, Noel Warner, Ted Young, Laurence Marton
No abstract text is available yet for this article.
November 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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